JOURNAL OF ANALYTICAL CHEMISTRY
·
2025
·Vol. 80
·期 7
·1274-1282
·
IF 1.1
Guan Zhimin,Xie Yaoyao,Li Ke,An Junling,Wang Ping
A ratiometric electrochemical aptasensor for the sensitive detection of mucin 1 ( MUC1 ) was designed based on a quadruped DNA walker driven by catalytic hairpin assembly ( CHA ). With the tetrahedral DNA nanostructure as the framework, the four legs that extend out acted as the walking chains. MUC1 bound specifically to the aptamer, activating the quadruped DNA walker and initiating the CHA reaction. The activated quadruped DNA walker hybridized with hairpin DNA1 labeled with methylene blue ( MB-HP1 ) immobilized on the electrode surface, and the toehold area of the MB-HP1 probe was exposed to bind with hairpin DNA2 labeled with ferrocene ( Fc-HP2 ), causing the quadruped DNA walker to be released. It continued to move on the electrode surface and bound with another MB-HP1. Eventually, a large number of electrochemical signal molecules were brought close to the electrode surface, while MB moved farther away. Under optimal conditions, the peak current ratio ( I Fc / I MB ) was linearly correlated with the logarithm of MUC1 concentration from 1 fg/mL to 1 ng/mL, with a detection limit as low as 0.56 fg/mL. In addition, the sensing platform demonstrated good performance in the analysis of serum samples.
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MEDICAL SCIENCE MONITOR
·
2018
·
IF 1.894
Zeng Li,Xiao-chang Liu,Rui Li,Jun Chang
Background Beta-secretase 1 (BACE1) is a rate-limiting enzyme in the generation of amyloid beta peptides, which are associated with Alzheimer’s disease (AD). It has been reported that Schisandrin B could improve cognitive functions in animal models of AD, but the underlying mechanisms are not completely understood. Material/Methods In this research, in order to investigate the effects of Schisandrin B on amyloid-β (Aβ) metabolism and its mechanisms, amyloid precursor protein (APP) and its proteolytic products were determined by enzyme-linked immunosorbent assay (ELISA), western blotting, and RT-PCR after incubation of N2a/Swe cells with Schisandrin B. Results The results indicated that Schisandrin B can significantly reduce the level of secretion of Aβ 40 and Aβ 42 secreted in N2a/Swe cells. Additionally, there was nonsignificant change in APP level after Schisandrin B treatment. Treatment of Schisandrin B dramatically reduced the mRNA and protein expression levels of BACE1. Moreover, Schisandrin B treatment resulted in a reduction of protein level of sAPPβ, an APP fragment cleavage by BACE1. Conclusions These results suggest that Schisandrin B inhibits the transcription and translation of BACE1, suppresses the activity of BACE1, and ultimately attenuates Aβ generation, which provides a novel mechanism for the regulation of Aβ metabolism by Schisandrin B.
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JOURNAL OF MATERIALS SCIENCE
·
2024
·Vol. 59
·期 7
·3177-3190
·
IF 3.5
Lu Xiaohui,Yu Shouwu,Gao Tian,Chen Yifu,Zhao Xiang,Xiao Shujuan
The wettability of the membrane surface is very important for the separation of oil-in-water emulsion. In this paper, the hydrophilic NH 2 -CAU-1 was added to the membrane surface by interfacial polymerization, which greatly improved the separation efficiency of oil-in-water emulsion. The effect of the amount of NH 2 -CAU-1 on the separation efficiency of TFN membrane was studied, and it was determined that TFN-1 membrane had ultra-high separation efficiency of oil-in-water emulsion (> 97.60%). TFN-1 membrane has a high underwater oil contact angle (higher than 139.53°), resulting in excellent oil adhesion resistance on the membrane surface. The universality and cycling performance of TFN-1 membrane were tested, and UWOCA had good stability under strong acid, strong base and different NaCl concentrations, and the separation efficiency did not change significantly after 8 cycles. The results show that TFN-1 membrane has excellent performance in separating oil-in-water emulsions and has broad application prospects in separating oil-in-water emulsions stabilized by surfactants. Graphical abstract
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Protection of Metals and Physical Chemistry of Surfaces
·
2020
·Vol. 56
·期 1
·180-188
·
IF 0.985
Li Min,Li Ying
— In this work, it found that zinc oxide (ZnO) and zinc chloride (ZnCl 2 ) can be dissolved well in 1-methylimidazolium trifluoromethylsulfonate (MIMTfO) ionic liquid with a high concentration (1.5 mol L −1 ). The electrochemical behavior of Zn(II) in MIMTfO ionic liquid containing ZnO or ZnCl 2 was investigated at 373 K. Cyclic voltammetry results indicated that the electrodeposition of zinc from both ZnO and ZnCl 2 is a one-step and two-electron reaction. Chronoamperometry studies revealed that the electrodeposition of zinc from both ZnO and ZnCl 2 is fit instantaneous nucleation, and the average values of the diffusion coefficient of Zn(II) were determined to be approximately 4.1 × 10 –8 cm 2 s –1 and 7.7 × 10 –9 cm 2 s –1 , respectively, at 373 K. The electrodeposition experiments were performed at 373 K and 383 K using potentiostatic electrolysis. The SEM images showed that dense and compact electrodeposits were obtained at 373 K in MIMTfO ionic liquid for both Zn(II) sources, and the EDS and XRD analysis confirmed that the obtained electrodeposits are metallic zinc.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2025
·
IF 3.9
Guojun Lv,Linfang Jiang,Yan Chen,Xuyang Zou,Xubin Zhang,Fumin Wang
The addition of an additional mesoporous/macroporous templating agent inevitably has an adverse effect on the economy and greenness of the seed-assisted synthesis process for hierarchical zeolites; hence, the development of an efficient seed-assisted method for the synthesis of hierarchical zeolite without the addition of an additional additive is appealing. Herein, we reported the preparation of hierarchical TS-1 zeolite with a large amount of mesopores using a liquid–solid mixture seed-assisted method and its application as a heterogeneous catalyst in the oxidative desulfurization reactions. The characterization results disclosed that the liquid–solid mixture seed was more conducive to the formation of hierarchical TS-1 zeolite than liquid seed alone and solid seed alone. The formation process of hierarchical TS-1 zeolite with different crystallization times was investigated in detail, and accordingly, a possible two-stage crystallization mechanism was proposed. In addition, the synthesized hierarchical TS-1 zeolite showed remarkably enhanced catalytic activity in the oxidative desulfurization of dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT) in comparison to the conventional microporous TS-1 zeolite. This work provides some guidance for the development of a more efficient and greener seed-assisted synthesis method for hierarchical zeolites without the addition of any additional mesoporous/macroporous templating agent.
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Gels
·
2025
·Vol. 11
·期 8
·568
·
IF 5.3
Yi Wang,Enkai Lin,Ze Wang,Tong Feng,An Xie
Electrochromic (EC) devices are gaining increasing attention for next-generation smart windows and low-power displays due to their reversible color modulation, low operating voltage, and flexible form factors. Recently, electrochromic energy storage devices (EESDs) have emerged as a promising class of multifunctional systems, enabling simultaneous energy storage and real-time visual monitoring. In this study, we report a flexible dual-functional EESD constructed using polyaniline (PANI) films doped with anthraquinone-1-sulfonic acid sodium salt (AQS), coupled with a redox-active PVA-based gel electrolyte also incorporating AQS. The incorporation of AQS into both the polymer matrix and the gel electrolyte introduces synergistic redox activity, facilitating bidirectional Faradaic reactions at the film–electrolyte interface and within the bulk gel phase. The resulting vertically aligned PANI-AQS nanoneedle films provide high surface area and efficient ion pathways, while the AQS-doped gel electrolyte contributes to enhanced ionic conductivity and electrochemical stability. The device exhibits rapid and reversible color switching from light green to deep black (within 2 s), along with a high areal capacitance of 194.2 mF·cm−2at 1 mA·cm−2and 72.1% capacitance retention over 5000 cycles—representing a 31.5% improvement over undoped systems. These results highlight the critical role of redox-functionalized gel electrolytes in enhancing both the energy storage and optical performance of EESDs, offering a scalable strategy for multifunctional, gel-based electrochemical systems in wearable and smart electronics.
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PLANT PHYSIOLOGY
·
2025
·Vol. 198
·期 4
·
IF 6.9
Zhang Xiaobo,Wang Ying,Fu Yao,Sun Ying,Zhang Xuefei,Tian Weijiang,Li Yangyang,Zhu Xiaoyan,Wang Nan,Song Hongyuan,He Guanghua,Sang Xianchun
Brassinosteroids (BRs) are polyhydroxylated steroid phytohormones that regulate important agronomic traits, including tiller number in rice (Oryza sativa L.), although the underlying molecular mechanisms remain unclear. Here, we isolated the rice mutant fewer tillers and dwarf 1 (ftd1). Map-based cloning revealed that FTD1 encodes a plant-specific GRAS family protein harboring a conserved GRAS domain at its C-terminus. Genetic and biochemical analyses showed that FTD1 physically interacts with MONOCULM 1 (MOC1) and inhibits its degradation. Genetic analysis also indicated that FTD1 functions as a positive regulator in the BR signaling pathway. Furthermore, OsGSK2 interacts with and phosphorylates FTD1 for degradation via the 26S proteasome. We determined that BRs promote FTD1 accumulation by suppressing OsGSK2 phosphorylation, thus enhancing MOC1 stability for increased tiller number. Our findings reveal an OsGSK2-FTD1-MOC1 regulatory cascade that mediates BR signaling in regulating rice tiller development.
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npj Biofilms and Microbiomes
·
2025
·Vol. 11
·期 1
·1-17
·
IF 9.2
Yang Ruili,Xu Yi,Xu Jinchun,Huang Chengqing,Zhu Feng,Wang Tian,Kong Rui,Xiao Jie,He Biao,Gu Xiaozhen,Wang Hui-Li
As a prevalent neurodevelopmental disease, whether ASD (autism spectrum disorder) can be ameliorated by the early use of a single microbe remains unknown. Here we used a lactobacillus strain, Lacticaseibacillus rhamnosus GR-1 (LGR-1), for prenatal intervention in autism-like mice with either environmental or idiopathic origins by exclusively administering to the pregnant dams at a dose of 10 9 /mouse/day, followed by offspring behavioral assessment with 3-chamber trial and marble burying test. The results revealed that LGR-1 prevented the occurrence of autism-like symptoms, as evidenced by the improved behaviors and restored E/I (excitatory-inhibitory) balance in the prefrontal cortex of male pups. In parallel, the offspring microbiome was reshaped by LGR-1 treatment, probably mediated by the vertical transmission of maternal microbiome, with its roles further unraveled by fecal microbiota transplant and cross-fostering experiments. In addition to gut commensals, the LGR-1-shaping vaginal microbiota also contributed to the establishment of “beneficial” microbiome. Regarding key taxa in offspring, Akkermansia muciniphila was influenced by LGR-1 and exerted impact on behaviors via pathways related to IL-17-producing lymphocytes. Our findings demonstrate that prenatal microbial administration protects offspring against autism-like behavioral phenotypes through microbiome transmission, highlighting a potential microbe-based therapeutic avenue to mitigate ASD risk.
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Materials
·
2025
·Vol. 18
·期 5
·1131
·
IF 3.2
Tian Zhao
HKUST-1 (HKUST = Hong Kong University of Science and Technology) is one of the most recognized metal-organic frameworks (MOFs) based on copper and trimesate, extensively studied for a variety of applications, such as gas storage, separation, adsorption, electrocatalysis, drug delivery, sensor and photodegradation, etc. In this work, we introduce a novel nanofused HKUST-1, referred to as N-CuBTC (BTC = trimesate), which has been synthesized with the hydrothermal method at room temperature (typical synthesis temperature is from 80~120 °C). The resulting N-CuBTC features an irregular particle morphology, with numerous crystals clustering together and edges that have fused, creating a hierarchical pore structure. In contrast to the traditional micro-sized octahedral HKUST-1 (named as M-CuBTC), N-CuBTC displays a unique clumped morphology, where the HKUST-1 crystals are seamlessly integrated into a cohesive structure. This innovative formation significantly enhances mass transfer capabilities and porosity accessibility. Consequently, N-CuBTC demonstrates markedly improved catalytic performance in the cyanosilylation of aldehydes.
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Nano Today
·
2025
·
IF 10.9
Yating Qin,Yan Lin,Chao Tian,Yujie Qi,Shuling Wang,Xiaoyuan Chen,Wenxing Gu
Immune checkpoint blockade (ICB) therapy has emerged as a promising avenue for the treatment of malignant tumors. Nonetheless, the efficacy of this approach is constrained by low anti-tumor immunity and restricted intratumoral delivery of immune checkpoint inhibitors (ICIs) in solid tumors. In this study, a pH-responsive nanocomplex (BRM/PTX/aPD-1) was developed for the active transport of aPD-1 and PTX, thereby enhancing cancer chemoimmunotherapy. BRM/PTX/aPD-1 was prepared by using dynamic and reversible benzoic-imine bonds cross-linked bovine albumin (BSA), anti-PD-1 antibody (aPD-1) and PTX-loaded polylysine dendrimers. In the acidic tumor microenvironment, BRM/PTX/aPD-1 exhibited a charge reversal from negative to positive due to the hydrolysis of the β-carboxylic amide structure, which triggered cation-induced transcytosis. This process enabled active transport of adequate amounts of BRM/PTX/aPD-1 to the tumor parenchyma, where it was degraded by further hydrolysis of benzoic-imine bonds and effectively released aPD-1 and PTX. The nanocomplex demonstrated notable intratumoral permeability both in vitro and in vivo , particularly enhancing the delivery of aPD-1. Additionally, the nanocomplex showed robust anti-tumor activity in an orthotopic breast cancer model, significantly inhibiting tumor proliferation and preventing the progression of lung metastasis. This pH-responsive nanocomplex presents a promising platform for improved intratumoral delivery of ICIs and emerges as a practical solution for overcoming tumor immunosuppression.
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Antioxidants
·
2025
·Vol. 14
·期 4
·468
·
IF 6.6
Xin Zhang,Shuang Han,Zhu Zeng,Jie Dai,Yi Jia
Selenium-binding protein 1 (SELENBP1) has been implicated in cancer development, neurological disorders, tissue injury, metabolic regulation, and cell differentiation. Sepsis is characterized prominently by immunological dysregulation and severe organ damage. However, whether SELENBP1 improves sepsis by regulating immune cell activity remains unknown. Here, we detected an elevation of SELENBP1 levels in the blood of sepsis patients and in the livers of septic mice. Significantly, SELENBP1 knockout (KO) prolonged survival in septic mice. This phenomenon was accompanied by decreased liver damage, reduced inflammation levels, and an increased regulatory T cell/T helper 17 cell (Treg/Th17) ratio in the spleen. Additionally, SELENBP1 deficiency induced a redox imbalance and inhibited dendritic cell (DC) maturation, resulting in a tolerogenic DC (tolDC) phenotype and an increase in the Treg/Th17 ratio. Furthermore, SELENBP1-KO mature DCs (mDCs) alleviated liver injury by increasing the Treg/Th17 ratio in the spleen, thus improving the survival of septic mice. These findings indicate that SELENBP1 is involved in sepsis by regulating DC immune activity, which might provide a potential way for sepsis treatment.
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ACS Nano
·
2025
·
IF 16
Hairui Huang,Hanyu Wu,Yizhou Zhang,Xunda Feng
Molecular-scale adsorption, catalysis, and separation demand nanoporous materials with high permeability, extensive surface areas, and pronounced nanoconfined effects. Fabricating polymeric particles with 3-D interwoven pores of ∼1 nm potentially addresses these needs. However, significant challenges remain in controlling their pore interconnectivity, uniformity, and achieving faceted particle shapes. Herein we present facile fabrication of polyhedral particles possessing interpenetrating 1 nm pores by suspension polymerization of double-gyroid (DG) liquid crystalline droplets. Mechanical stirring of the disordered phase at elevated temperatures, followed by undercooling, leads to the emulsification of DG droplets, as confirmed by synchrotron small-angle X-ray scattering (SAXS). UV-induced cross-linking of the DG droplets preserves the ordered network of 1 nm pores, as characterized by SAXS and microscopy. Intriguingly, due to the elasticity induced by the Ia3̅d periodicities, these particles adopt polyhedral shapes to avoid the elastic energy penalty associated with conventional sphericity. We demonstrate that these faceted particles, featuring 1 nm pores and efficient packing, enable rapid, size-exclusive adsorption and nanoconfined degradation of organic pollutants, driven by their 3-D permeability, high surface area, and enhanced nanoconfinement effects.
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