Biotechnology for Biofuels and Bioproducts
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2024
·Vol. 17
·期 1
·1-12
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IF 3.3
Zhang Bin,Gou Kexin,Xu Kexin,Li Zhimin,Guo Xiaoyan,Wu Xiaoyu
Background β-Arbutin, a hydroquinone glucoside found in pears, bearberry leaves, and various plants, exhibits antioxidant, anti-inflammatory, antimicrobial, and anticancer effects. β-Arbutin has wide applications in the pharmaceutical and cosmetic industries. However, the limited availability of high-performance strains limits the biobased production of β-arbutin. Results This study established the β-arbutin biosynthetic pathway in C. glutamicum ATCC13032 by introducing codon-optimized ubiC , MNX1 , and AS . Additionally, the production titer of β-arbutin was increased by further inactivation of csm and trpE to impede the competitive metabolic pathway. Further modification of the upstream metabolic pathway and supplementation of UDP-glucose resulted in the final engineered strain, C. glutamicum AR11, which achieved a β-arbutin production titer of 7.94 g/L in the optimized fermentation medium. Conclusions This study represents the first successful instance of de novo β-arbutin production in C. glutamicum , offering a chassis cell for β-arbutin biosynthesis.
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BIOORGANIC CHEMISTRY
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2017
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IF 3.231
Zhenhua Wu,Wenxia Liao,Kelin Chen,Jingfang Qin,Huang Tang
A series of 6- and 9-substituted cleistopholine derivatives has been designed, synthesized and investigated to inhibit the aggregation of acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and β-myloid (A β). Results showed that these synthetic compounds had excellent AChE inhibitory activity and a significant in vitro inhibitory potency toward the self-induced A β aggregation. When SH-SY5Y cells were treated with these substituted cleistopholine derivatives during they overexpressed the Swedish mutant form of human β -amyloid precursor protein (APPsw), A β 42 secretion levels were significantly reduced. According to a parallel artificial membrane permeation assay for BBB, seven out of these sixteen synthetic compounds probably could cross the blood-brain barrier (BBB) to reach their targets in the central nervous system (CNS).
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Journal of Energy Storage
·
2024
·
IF 8.9
Rong Han,Yusong Pan,Chao Du,Yanlei Xiang,Yuanqing Wang,Hongwu Zhu,Chengjie Yin
Aqueous zinc ion batteries (AZIB) have become a research hotspot for energy storage systems (ESS) in recent years due to some advantages such as their low risk and low cost. However, the structural collapse during charging and discharging affects the reversibility of the battery , which is a very serious blow to manganese-based materials and will affect their subsequent development in batteries. Therefore, β-MnO 2 cathode materials doped by the rare earth element europium (Eu) were prepared by a simple hydrothermal method . Compared with β-MnO 2 , Eu-doped MnO 2 possessed a high specific capacity of 409 mA h g −1 at 0.2 A g −1 . The initial specific capacity of Eu-doped MnO 2 at 1 A g −1 was 200.23 mA h g −1 and the maximum discharge specific capacity of 254 mA h g −1 was reached at 128 cycles. The energy storage mechanism of the electrodes was investigated by ex-situ XRD and ex-situ XPS tests. It was found that Eu element doping to β-MnO 2 not only maintains the stability of the crystal structure during redox reaction, but also promotes the embedding reaction of H + /Zn 2+ . The combination of rare earth elements Eu and MnO 2 is a new direction in the development path of aqueous zinc ion batteries.
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JOURNAL OF MEMBRANE SCIENCE
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2025
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IF 9
Xuan Wang,Jiaojiao Duan,Weiliang Li,Xiaoli Wu,Yong Wang,Mingjie Wei,Yifan Li,Runnan Zhang,Jingtao Wang
Covalent organic framework (COF) materials hold significant promise for membrane technology due to their precisely defined porous structures and tunable functionalities. However, the stability of COF membranes in organic solvents, critical for long-term operation, has seldom been studied and remains unclear. This study synthesizes three β-ketoenamine type COF membranes with different amounts of sulfonate groups via vacuum-assisted assembly and systematically evaluates their stability in four typical organic solvents (alcohol, ketone, ester, and alkane), respectively. We investigated the time-dependent membrane morphologies, thickness swelling, and long-term organic solvent nanofiltration (OSN) performance within a period of up to 180 days. Although the COF membranes suffer from a certain degree of structural change and permeance decline during the initial testing period, they can finally achieve quite stable separation performance. The effects of solvent type and the number of –SO 3 H groups on membrane stability are systematically discussed. Based on the evaluation methods adopted in this study, the prospects of COF membranes for long-time application in organic solvent can be highlighted, but some critical problems are also raised. We also investigate the swelling behavior under harsh conditions (e.g. elevated temperature, strong acids, and alkalis) to accelerate the swelling process, which is found to enable fast and effective evaluation of the long-term stability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Liang Li,Yajie Zhao,Anqiong Mou,Xin Shen,Zhendong Liu,Zhang Luo
Non-starch polysaccharides are added to grains as dietary fiber to enhance their processing characteristics. This study investigates the influence of highland barley β-glucan (HBBG) on the gelatinization, texture, rheological, structural, and retrogradation characteristics of corn starch (CS). The pasting analysis revealed that the trough, peak, and final viscosities of the CS gel were markedly decreased after HBBG addition. Rheological analysis indicated reduced viscosity, flow behavior, and elasticity of the gels following HBBG addition. Fourier Transform Infrared Spectroscopy (FTIR) displayed a red shift in wavenumber, suggesting increased hydrogen bonding interactions induced by HBBG. HBBG increased water holding in CS and reduced hardness by forming hydrogen bonds with the starch. Specifically, the hardness of CS containing 20 % HBBG decreased from 290.32 g to 66.1 g. X-ray diffraction indicated 7.98 and 8.58 % reductions in CS gels with 20 % HBBG after 24 h and 7 days of retrogradation, respectively. These findings offer valuable insights for the formulation of functional foods designed for elderly individuals.
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JOURNAL OF HAZARDOUS MATERIALS
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2024
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IF 12.2
Xiaoli Gou,Yongchun Fu,Juan Li,Juan Xiang,Minghui Yang,Yi Zhang
Nanoplastics , widely existing in the environment and organisms, have been proven to cross the blood-brain barrier, increasing the incidence of neurodegenerative diseases like Alzheimer’s disease (AD). However, current studies mainly focus on the neurotoxicity of nanoplastics themselves, neglecting their synergistic effects with other biomolecules and the resulting neurotoxicity. Amyloid β peptide (Aβ), which triggers neurotoxicity through its self-aggregation, is the paramount pathogenic protein in AD. Here, employing polystyrene nanoparticles (PS) as a model for nanoplastics, we reveal that 100 pM PS nanoparticles significantly accelerate the nucleation rate of two Aβ subtypes (Aβ 40 and Aβ 42 ) at low concentrations, promoting the formation of more Aβ oligomers and leading to evident neurotoxicity. The hydrophobic surface of PS facilitates the interaction of hydrophobic fragments between Aβ monomers , responsible for the augmented neurotoxicity. This work provides consequential insights into the modulatory impact of low-dose PS on Aβ aggregation and the ensuing neurotoxicity, presenting a valuable foundation for future research on the intricate interplay between environmental toxins and brain diseases.
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NEW JOURNAL OF CHEMISTRY
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2024
·Vol. 48
·期 36
·15884-15893
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IF 2.7
Dong Ju Zhou,Wei Ming Liu,Su Ping Dai,Shuai Qiang Jiang,Yin Wang,Jia Jia Yang,Ya Wei Chen,Jun Liang Chen,Hyun Jin Park,Hui Yun Zhou
In our work, serine-modified β-cyclodextrin (Ser-β-CD) was synthesized and a berberine hydrochloride (BBH)/Ser-β-CD inclusion complex (IC) was prepared using the freeze-drying method. Phase solubility studies showed that BBH and Ser-β-CD formed stable inclusion complexes in a stoichiometric ratio of 1 : 1. The IC was characterized using SEM and FT-IR analyses. The results of molecular docking showed that BBH was completely contained in the Ser-β-CD cavity and the binding energy was −7.87 kcal mol−1. The results of in vitro release studies indicated that BBH could be released slowly from the IC. In addition, the prepared Ser-β-CD and IC had good biocompatibility. The IC demonstrated excellent antibacterial activity against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Therefore, the BBH/Ser-β-CD IC has potential application prospects in biomedicine and food industry.
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COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2025
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IF 5.4
Yu Yang,Xianbing Song,Manman Wang,Xiaolian Li,Ziqiang Wang,Peng Wang
β-Alanine is an important non-essential amino acid and used in the manufacture of a wide range of drugs, in particular it plays a key role in the biosynthesis of carnosine and pantothenic acid. L-aspartate-α-decarboxylase (PanD) is the key enzyme in the enzymatic preparation of β-alanine. In this study, BsPanD* was modified by protein structure prediction and directed evolution, and a mutant BsPanD* PQ4 with good catalytic properties was obtained, which showed a 40 % increase in activity and remained stable in the pH 6.0–9.0 range. The strain E.coli pRSF Duet-1 - smaiA - ecaspA (pMA) was constructed for efficient co-expression of maleate cis - trans isomerase and L-aspartase, with an apparent activity of 253.4 U/g. The multi-enzyme cascade catalytic process for the preparation of β-alanine with maleate as the substrate was developed. The results showed that when the concentration of ammonium maleate was 1 mol/L, the substrate conversion rate was greater than 99.9 %, and the β-alanine concentration was 87.5 g/L with a yield of 98.3 %. This study not only solved the problem of low activity and poor stability of PanD, but also realized the preparation of β-alanine from maleate, a cheap chemical raw material, and laid the foundation for its industrial application.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Keyu Lin,Zhongyu Shi,Zheyi Zhang,Yunfan Wei,Sibao Wan,Haiyan Gao,Zhen Qin
β-1,3-1,4-Glucanases have attracted significant interest because of their potential applications in various food industries. Glycoside hydrolase (GH) family 148 β-1,3-1,4-glucanases exhibit excellent thermal stability and a unique catalytic mechanism, making them particularly promising for certain food processing applications. This study investigated the module architecture enzymatic properties, catalytic mechanisms, and potential applications of a multi-domain β-1,3-1,4-glucanase (EngU) from GH family 148. The overall structure of EngU comprises three independently folding domains, namely the GH148 catalytic domain, CBM4, and the C-terminal domain. The insertion of CBM4 causes the catalytic domain of EngU to be non-contiguous in sequence. The optimum reaction conditions for EngU have been found to be pH 6.0 and 90 °C, demonstrating relatively high thermostability. EngU is an atypical β-1,3-1,4-glucanase, predominantly cleaves the β-1,3 glycosidic bonds in β-1,3-1,4-glucan. Malt saccharification experiments revealed that adding EngU (80 U/g malt) can decreased the viscosity of mash by 6.85 % and the filtration time by 28.83 %. Furthermore, EngU was found to effectively hydrolyze oat bran, producing β-glucooligosaccharides, with the main hydrolysis products being trisaccharide and disaccharide. These results demonstrate the unique module architecture and the application potential of GH family 148 β-1,3-1,4-glucanases, providing valuable insights and resources regarding this category of glycoside hydrolases.
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ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2018
·
IF 3.118
Yichuan Xiao,Jiantao Ye,Ying Zhou,Junjun Huang,Xiawen Liu,Biyun Huang,Liu Zhu,Bo Wu,Genshui Zhang,Yi Cai
AMP-activated protein kinase (AMPK) is a central regulator of multiple metabolic pathways. It has been shown that activation of AMPK could inhibit fibroblast proliferation and extracellular matrix (ECM) accumulation, thereby suppressing cardiac fibrosis. Baicalin , the major component found in skullcap, possesses multiple protective effects on the cardiovascular system. However, little is known about the effect of baicalin on cardiac fibrosis and the molecular mechanism by which baicalin exerts its anti-fibrotic effects has not been investigated. In this study, we revealed that baicalin could inhibit cell proliferation , collagen synthesis , fibronectin (FN) and Connective tissue growth factor (CTGF) protein expression in cardiac fibroblasts induced by angiotensin Ⅱ (Ang Ⅱ). It also ameliorated cardiac fibrosis in rats submitted to abdominal aortic constriction (AAC). Moreover, baicalin inhibited transforming growth factor-β (TGF-β)/Smads signaling pathway stimulated with Ang Ⅱ through activating AMPK. Subsequently, we also demonstrated that baicalin attenuated Ang Ⅱ-induced Smad3 nuclear translocation, and interaction with transcriptional coactivator p300, but promoted the interaction of p300 and AMPK. Taken together, these results provide the first evidence that the effect of baicalin against cardiac fibrosis may be attributed to its regulation on AMPK/TGF-β/Smads signaling, suggesting the therapeutic potential of baicalin on the prevention of cardiac fibrosis and heart failure.
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SENSORS
·
2018
·Vol. 18
·期 3
·681
·
IF 2.475
Xiaoyang Du,Xiaoxia Zhang,Chunlai Jiang,Weilu Zhang,Lizhu Yang
A novel fluorescence sensor of NR-β-CD@AuNPs was prepared for the trace detection of nitrite in quantities as low as 4.25 × 10−3μg∙mL−1in an aqueous medium. The fluorescence was due to the host-guest inclusion complexes between neutral red (NR) molecules and gold nanoparticles (AuNPs), which were modified by per-6-mercapto-beta-cyclodextrins (SH-β-CDs) as both a reducing agent and a stabilizer under microwave radiation. The color of the NR-β-CD@AuNPs changed in the presence of nitrite ions. A sensor was applied to the determination of trace nitrites in environmental water samples with satisfactory results.Keywords:gold nanoparticles;cyclodextrin;neutral red;nitrite ions;fluorescence sensor;trace detection
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CHEMOSPHERE
·
2018
·
IF 4.427
Xinxing Zhou,Yunzhi Xu,Xiaojie Mei,Ningjie Du,Rongmao Jv,Zhaoxia Hu,Shouwen Chen
An efficient and inexpensive catalyst for oxygen reduction reaction (ORR), polyaniline (PANI) and β-MnO 2 nanocomposites (PANI/β-MnO 2 ), was developed for air-cathode microbial fuel cells (MFCs). The PANI/β-MnO 2 , β-MnO 2 , PANI and β-MnO 2 mixture modified graphite felt electrodes were fabricated as air-cathodes in double-chambered MFCs and their cell performances were compared. At a dosage of 6 mg cm −2 , the maximum power densities of MFCs with PANI/β-MnO 2 , β-MnO 2 , PANI and β-MnO 2 mixture cathodes reached 248, 183 and 204 mW m −2 , respectively, while the cathode resistances were 38.4, 45.5 and 42.3 Ω, respectively, according to impedance analysis. Weak interaction existed between the rod-like β-MnO 2 and surficial growth granular PANI, this together with the larger specific surface area and PANI electric conducting nature enhanced the electrochemical activity for ORR and improved the power generation. The PANI/β-MnO 2 nanocomposites are a promising cathode catalyst for practical application of MFCs.
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