Frontiers of Medicine
·
2021
·Vol. 15
·期 6
·887-902
·
IF 4.592
Wu Xin,Wang Zongqi,Li Haiying,Xie Xueshun,Wu Jiang,Shen Haitao,Li Xiang,Wang Zhong,Chen Gang
Monocarboxylic acid transporter 1 (MCT1) maintains axonal function by transferring lactic acid from oligodendrocytes to axons. Subarachnoid hemorrhage (SAH) induces white matter injury, but the involvement of MCT1 is unclear. In this study, the SAH model of adult male Sprague-Dawley rats was used to explore the role of MCT1 in white matter injury after SAH. At 48 h after SAH, oligodendrocyte MCT1 was significantly reduced, and the exogenous overexpression of MCT1 significantly improved white matter integrity and long-term cognitive function. Motor training after SAH significantly increased the number of ITPR2 + SOX10 + oligodendrocytes and upregulated the level of MCT1, which was positively correlated with the behavioral ability of rats. In addition, miR-29b and miR-124 levels were significantly increased in SAH rats compared with non-SAH rats. Further intervention experiments showed that miR-29b and miR-124 could negatively regulate the level of MCT1. This study confirmed that the loss of MCT1 may be one of the mechanisms of white matter damage after SAH and may be caused by the negative regulation of miR-29b and miR-124. MCT1 may be involved in the neurological improvement of rehabilitation training after SAH.
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Scientific Reports
·
2025
·
IF 3.9
Xu Qiuling,Liu Zhuoxi,Wang Liyuan,Liu Guoyang,Zhao Long,Yue Hui,Liu Yong
Diabetic cardiomyopathy (DCM) requires a systematic approach for effective management. Focusing on key signaling pathways in myocardial fibrosis could offer novel therapeutic approaches for DCM. The objective of this research is to investigate new biomarker and the underlying mechanisms by which baicalin intervenes in the progression of DCM. Swiss Target Prediction databases and weighted correlation network analysis (WGCNA) were employed to collect and analyze targets of baicalin and DCM. Intersection targets were obtained through venn online website, network visualization was conducted through STRING database and Cytoscape 3.7.2 software. Molecular docking validation was completed via AutoDock Vina software. Western blotting, RT-qPCR and histology were carried out to validate the direct targets of baicalin and explore the molecular mechanism during DCM progression. In mice induced to DCM, we observed that disruptor of telomeric silencing 1-like (DOT1L) was upregulated. Baicalin (BC) reduced myocardial fibrosis in mice as indicated by the reduced expression levels of fibrosis markers COL-1 and TGF-β. The inhibitory effect of BC was reversed by overexpressed DOT1L and further enhanced by silenced DOT1L. Our results indicate that cardiomyocyte DOT1L exerts a pathogenic role in DCM and BC specifically targets DOT1L/COL-1 signaling to reduce myocardial fibrosis in DCM.
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JOURNAL OF LOSS PREVENTION IN THE PROCESS INDUSTRIES
·
2025
·
IF 4.2
Junjie Gu,Xinglin Wen,Yong Pan,Lei Ni
1-methyl-2,4-cyclohexanediamine (2,4-MCHD) is the core raw material for the synthesis of high-grade polyurethane. Aiming at the problems of long reaction time and safety risks in batch reactors, a fast and safe synthesis scheme of 2,4-MCHD was proposed. The one-pot catalytic hydrogenation of 2, 4-dinitrotoluene (2, 4-DNT) to 2, 4-MCHD was investigated in a microfilled bed reactor (μPBR) over the 5%LiOH-5%Ru/γ-Al 2 O 3 catalyst. The effects of temperature, pressure, gas and liquid volumetric were investigated. Under the optimized condition (180 °C, 7 MPa H 2 pressure, 0.6 mL/min liquid flow rate, 40 mL/min gas flow rate), within a residence time of 144 s, the conversion of 2,4-DNT and the selectivity of 2,4-MCHD exceeded 99% and 80%, respectively. Compared to the conventional batch mode, an increase of one to two orders of magnitude in space-time-yield (STY) was realized under continuous flow mode. Furthermore, the inherent risks of high-pressure hydrogenation in batch processes are significantly mitigated in the μPBR due to its minimal hydrogen inventory and superior heat and mass transfer characteristics.
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Materials Science and Engineering B-Advanced Functional Solid-State Materials
·
2025
·
IF 4.6
Huiping Wu,Hui Sun,Bin Yu,Afang Gao,Gaoyuan Chen,Xinyu Liu,Shijie Wang,Yutong Feng
Recently, the rapid growth of the textile, printing, and dyeing industries has led to severe pollution from dye wastewater. Bimetallic metal-organic frameworks (bimetallic MOFs) have emerged as promising adsorbents for dyes removal. Herein, Fe-HKUST-1 with an octahedral structure was anchored on the surface of viscose spunlaced nonwoven material (VSN) via an in situ solvothermal method to prepare Fe-HKUST-1@VSN. After carbonization, Fe-HKUST-1@VSNC was obtained for the adsorption of organic dyes in water. When the carbonization temperature is no more than 240 ο C, the morphologies and structures of Fe-HKUST-1@VSNC are not changed. And the adsorption efficiencies and capacities of Fe-HKUST-1@VSNC-240 for Congo red (CR) and crystal violet (CV) are the optimal. At an initial dye concentration of 10 mg L −1 , a temperature of 35 °C, and pH 7, Fe-HKUST-1@VSNC-240 shows its highest performance, reaching 98.17 % removal of CV in 90 min and 98.65 % removal of CR in 120 min. The adsorption processes of Fe-HKUST-1@VSNC-240 for both CV and CR are slightly affected by coexisting ions. The adsorption efficiency of Fe-HKUST-1@VSNC-240 for CV and CR declines to 50.10 % (after 6 cycles) and 56.76 % (after 22 cycles), respectively. Moreover, Fe-HKUST-1@VSNC-240 also demonstrates good adsorption performance in real water environment. The adsorption processes of Fe-HKUST-1@VSNC-240 for both CV and CR are endothermic, spontaneous, and monolayer adsorption, primarily through chemical adsorption and intra-particle diffusion. The synthesized Fe-HKUST-1@VSNC-240 can adsorb various organic dyes, along with excellent structural stability and recyclability, offering a promising solution for dye-contaminated wastewater remediation.
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Biomass Conversion and Biorefinery
·
2022
·
IF 4.05
Li Keying,Zhu Hengji,Zhao Yu,Yan Jingwei,Zhang Jiajian,Lin Xiuhua,Fan Liwei,Lu Zexiang
The solvent has an important impact on liquefaction conversion from biomass to efficiently obtain fuels and chemicals. In this paper, the catalytic liquefaction of woody biomass was studied using fir sawdust as raw material and concentrated sulfuric acid as catalyst in GVL/1-octanol mixed solvent with mechanical stirring and oil bath heating. The influence of GVL on liquefaction yield was discussed, and the liquefaction productions were characterized by some analysis methods to analyze the liquefaction mechanism. The results show that adding GVL benefits to improving liquefaction yield and generating high value-added monophenol because of the enhancement of the surface wettability of biomass and the inhibition of polymerization of mononuclear aromatics with other degradation products. The maximum liquefaction yield of 76.07% was obtained under the suitable conditions of fir sawdust 10 g, temperature 150 ℃, reaction time 60 min, mass ratio of sawdust/solvent 1:6, catalyst concentration 0.6 mol·L −1 , and mass ratio of GVL/1-octanol 4:6. The mechanism study reveals that aromatics originated by lignin degradation as well as small molecule ketones from cellulose and hemicellulose degradation. GVL/1-octanol as an eco-friendly solvent system exhibits a promising prospect of application in woody biomass conversion. Graphical abstract
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ACTA PHARMACOLOGICA SINICA
·
2024
·
IF 6.9
Wang Fei,Wang Yu,Zhang Qing-yang,Hu Ke-yu,Song Ying-jie,Yang Lin,Fei Fan,Xu Ceng-lin,Cui Sun-liang,Ruan Ye-ping,Wang Yi,Chen Zhong
Status epilepticus (SE), a serious and often life-threatening medical emergency, is characterized by abnormally prolonged seizures. It is not effectively managed by present first-line anti-seizure medications and could readily develop into drug resistance without timely treatment. In this study, we highlight the therapeutic potential of CZL80, a small molecule that inhibits caspase-1, in SE termination and its related mechanisms. We found that delayed treatment of diazepam (0.5 h) easily induces resistance in kainic acid (KA)-induced SE. CZL80 dose-dependently terminated diazepam-resistant SE, extending the therapeutic time window to 3 h following SE, and also protected against neuronal damage. Interestingly, the effect of CZL80 on SE termination was model-dependent, as evidenced by ineffectiveness in the pilocarpine-induced SE. Further, we found that CZL80 did not terminate KA-induced SE in Caspase-1 −/− mice but partially terminated SE in IL1R1 −/− mice, suggesting the SE termination effect of CZL80 was dependent on the caspase-1, but not entirely through the downstream IL-1β pathway. Furthermore, in vivo calcium fiber photometry revealed that CZL80 completely reversed the neuroinflammation-augmented glutamatergic transmission in SE. Together, our results demonstrate that caspase-1 inhibitor CZL80 terminates diazepam-resistant SE by blocking glutamatergic transmission. This may be of great therapeutic significance for the clinical treatment of refractory SE.
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JOURNAL OF ELECTRONIC MATERIALS
·
2017
·Vol. 46
·期 7
·4047-4051
·
IF 1.579
Li Chunchun,Luo Hao,Xiang Huaicheng,Tang Ying,Fang Liang
A series of low-firing Li 4(1+ x ) WO 5 ( x = 0.02, 0.04, 0.06, 0.08) ceramics were prepared using the solid-state reaction method. Effects of excess lithium on the crystal structure, sintering behavior, and microwave dielectric properties were studied. For x < 0.06 samples, only a single Li 4 WO 5 phase was formed, while a secondary phase detected as Li 6 WO 6 appeared in the x = 0.08 sample. The relative density increased obviously with increasing amount of excess lithium, resulting in the remarkable increase in quality factor. The sample with x = 0.04 sintered at 950°C obtained a high relative density of ∼95.1% and good microwave dielectric properties with ε
r
∼ 9.5, Q × f ∼ 50200 GHz (at 12.2 GHz), τ
f
∼ −3.2 ppm/°C.
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PLANT PHYSIOLOGY
·
2025
·Vol. 198
·期 4
·
IF 6.9
Zhang Xiaobo,Wang Ying,Fu Yao,Sun Ying,Zhang Xuefei,Tian Weijiang,Li Yangyang,Zhu Xiaoyan,Wang Nan,Song Hongyuan,He Guanghua,Sang Xianchun
Brassinosteroids (BRs) are polyhydroxylated steroid phytohormones that regulate important agronomic traits, including tiller number in rice (Oryza sativa L.), although the underlying molecular mechanisms remain unclear. Here, we isolated the rice mutant fewer tillers and dwarf 1 (ftd1). Map-based cloning revealed that FTD1 encodes a plant-specific GRAS family protein harboring a conserved GRAS domain at its C-terminus. Genetic and biochemical analyses showed that FTD1 physically interacts with MONOCULM 1 (MOC1) and inhibits its degradation. Genetic analysis also indicated that FTD1 functions as a positive regulator in the BR signaling pathway. Furthermore, OsGSK2 interacts with and phosphorylates FTD1 for degradation via the 26S proteasome. We determined that BRs promote FTD1 accumulation by suppressing OsGSK2 phosphorylation, thus enhancing MOC1 stability for increased tiller number. Our findings reveal an OsGSK2-FTD1-MOC1 regulatory cascade that mediates BR signaling in regulating rice tiller development.
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MicrobiologyOpen
·
2018
·Vol. 8
·期 1
·e00619
·
IF 2.682
Adnan B. Al-Hawash,Xiaoyu Zhang,Fuying Ma
Graphical Aspergillus sp. RFC-1 was obtained and its degradation ability for petroleum hydrocarbons was evaluated through surface adsorption, cell uptake, hydrophobicity, surface tension, biosurfactant production and emulsification activity. In addition, the degradation mechanism was investigated. The results indicated the strain RFC-1 showed high removal activity for PHs, including biodegradation, adsorption and emulsifiability. Petroleum pollution inevitably occurs at any stage of oil production and exerts a negative impact on the environment. Some microorganisms can degrade petroleum hydrocarbons (PHs). Polluted sludge of Rumaila oil field was use to isolate the highly efficient hydrocarbon-degrading fungal strain. Aspergillus sp. RFC-1 was obtained and its degradation ability for petroleum hydrocarbons was evaluated through surface adsorption, cell uptake, hydrophobicity, surface tension, biosurfactant production, and emulsification activity. In addition, the degradation mechanism was investigated. The results indicated the strain RFC-1 showed high removal activity for PHs, including biodegradation, adsorption, and emulsifiability. On the day 7 of incubation, the removal efficiencies of crude oil, naphthalene (NAP), phenanthrene (PHE), and pyrene (PYR) reached 60.3%, 97.4%, 84.9%, and 90.7%, respectively. Biodegradation efficiencies of crude oil, NAP, PHE, and PYR were 51.8%, 84.6%, 50.3%, and 55.1%, respectively. Surface adsorption and cell absorption by live mycelial pellets followed a decreasing order: PYR ≥ PHE > NAP > crude oil. Adsorption by heat-killed mycelial pellets increased within 40 and 10 min for crude oil and PAHs, respectively, and remained constant thereafter. Effects of cell surface hydrophobicity, surface tension, and emulsification index were discussed. Intra- and extracellular enzymes of strain RFC-1 played important roles in PHs degradation. The strain RFC-1 is a prospective strain for removing PHs from aqueous environments.
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Journal of Dental Sciences
·
2026
·Vol. 21
·期 4
·2375
·
IF 3.6
Jie Li,Changqing Yan,Xinyi Sun,Chenying Zhang,Xiaozhe Wang,Yang Liu,Shuguo Zheng
Background/purpose Cleidocranial dysplasia (CCD), caused by Runt-related transcription factor 2 (RUNX2) haploinsufficiency, features delayed tooth eruption. Although impaired osteoclastogenesis contributes to eruption failure, the direct molecular mechanisms linking RUNX2 deficiency to osteoclast dysfunction remain elusive. Sirtuin 1 (SIRT1) is a critical regulator of osteoclastogenesis; however, whether it serves as a downstream effector of RUNX2 remains unexplored. This study aimed to elucidate the mechanistic link between RUNX2 and SIRT1 in regulating osteoclast differentiation and tooth eruption. Materials and methods Tooth eruption and osteoclast differentiation were assessed by microcomputed tomography and histological analysis in Runx2 +/− mice. Chromatin immunoprecipitation quantitative polymerase chain reaction and luciferase reporter assays confirmed RUNX2 binding to the Sirt1 promoter. Osteoclast marker expression was evaluated by reverse transcription quantitative polymerase chain reaction and western blotting. Bone marrow-derived macrophages from Runx2 +/− mice were transfected with small interfering RNA targeting SIRT1 for rescue experiments. Osteoclast differentiation and function were assessed by tartrate-resistant acid phosphatase (TRAP) staining and bone resorption assays. Results Runx2 +/− mice showed significantly reduced mandibular first molar eruption and decreased TRAP-positive osteoclasts, accompanied by SIRT1 upregulation. RUNX2 directly bound to the Sirt1 promoter and repressed its transcription. SIRT1 knockdown increased expression levels of osteoclast markers and elevated acetylation of NF-κB p65 at lysine 310. Importantly, SIRT1 knockdown rescued osteoclast formation and resorption to wild-type levels in Runx2 +/− cells. Conclusion RUNX2 haploinsufficiency impairs tooth eruption through transcriptional derepression of SIRT1, which inhibits osteoclastogenesis via NF-κB p65 deacetylation, highlighting the RUNX2-SIRT1 axis as a novel therapeutic target for CCD.
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VIROLOGICA SINICA
·
2012
·Vol. 27
·期 1
·19-25
·
IF 0
Liang Yuan-Yuan,Wang Bin,Qian Dong-Meng,Li Ling,Wang Zhi-Hao,Hu Ming,Song Xu-Xia
To investigate the inhibitory effects of Ginsenoside Rb1 (GRb1) on apoptosis caused by Herpes Simplex Virus-1(HSV-1) in Human Glioma Cells (U251), U251 cells were infected by HSV-1 at a multiplicity of infection of 5 and GRb1, GRb1+HSV-1, HSV-1 and control groups. MTT and cell apoptosis assays were used to detect the inhibitory effects of GRb1 on the apoptosis of U251 cells that caused by HSV-1 infection for various concentrations of drug and virus treatments by MTT assay. We found that in the 400 μg/mL GRb1 and 400 μg/mL GRb1+HSV-1 groups, MTT values were higher than control group at all times ( P <0. 05). Moreover, the apoptosis rate in the 400 μg/mL GRb1+HSV-1 group was lower than the HSV-1 group ( P <0. 05). These results confirmed that, at appropriate concentrations, GRb1 could inhibit nerve cell apoptosis in HSV-1 infections.
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Frontiers in Genetics
·
2022
·
IF 4.772
Shan Mengjie,Liu Hao,Hao Yan,Song Kexin,Meng Tian,Feng Cheng,Wang Youbin,Huang Yongsheng
Background: Keloid is a skin fibroproliferative disease with unknown pathogenesis. Metabolomics provides a new perspective for revealing biomarkers related to metabolites and their metabolic mechanisms.Method: Metabolomics and transcriptomics were used for data analysis. Quality control of the data was performed to standardize the data. Principal component analysis (PCA), PLS-DA, OPLS-DA, univariate analysis, CIBERSORT, neural network model, and machine learning correlation analysis were used to calculate differential metabolites. The molecular mechanisms of characteristic metabolites and differentially expressed genes were identified through enrichment analysis and topological analysis.Result: Compared with normal tissue, lipids have a tendency to decrease in keloids, while peptides have a tendency to increase in keloids. Significantly different metabolites between the two groups were identified by random forest analysis, including 1-methylnicotinamide, 4-hydroxyproline, 5-hydroxylysine, and l-prolinamide. The metabolic pathways which play important roles in the pathogenesis of keloids included arachidonic acid metabolism and d-arginine and d-ornithine metabolism. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide are metabolic indicators of keloid severity. The high-risk early warning index for 5-hydroxylysine is 4 × 108-6.3×108 (p = 0.0008), and the high-risk predictive index for 1-methylnicotinamide is 0.95 × 107-1.6×107 (p = 0.0022).Conclusion: This study was the first to reveal the metabolome profile and transcriptome of keloids. Differential metabolites and metabolic pathways were calculated by machine learning. Metabolomic profiling reveals that 5-hydroxylysine and 1-methylnicotinamide may be metabolic indicators of keloid severity.
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