ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Huahui Gao,Xinmei Song,Qirong Chen,Ruo Yuan,Yun Xiang
The demethylase of fat mass and obesity related protein (FTO) is critical to regulate the dynamic N 6 -methyladenosine (m6A) modification of eukaryotic mRNAs, and its overexpression has found to be closely related to the initiation of several cancers. On the basis of a target-promoted specific activation of DNAzyme strategy coupled with self-primer exponential amplification reaction (SPEXPAR) cycles and DNA supersandwich assemblies, the highly sensitive and label-free electrochemical FTO assay approach is established. The modification of the catalytic core nucleobase of the DNAzyme probe by m6A can inhibit its cleavage activity. The presence of target FTO catalyzes the elimination of the methyl group to restore the DNAzyme activity, which cleaves the hairpin substrates to trigger the SPEXPAR for yielding many ssDNAs. The capture of these DNAs on the sensor electrode leads to the initiation of supersandwich assembly formation of long dsDNAs . Tremendous electrochemical signal probe of [Ru(NH 3 ) 6 ]Cl 3 are then absorbed on these dsDNAs to produce highly amplified catalytic currents with the assistance of K 3 [Fe(CN) 6 ] for detecting trace FTO with 63.1 fM detection limit. Furthermore, the sensor can be employed for selective assay of FTO in cell lysates, revealing the great potential of this sensing strategy for biomedical and biological study applications.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
·
IF 5.895
Ziyan Wang,Heping Cui,Mengyu Ma,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
The effect of ellagic acid on the formation of pyrazine, methylpyrazine, 2,3-methylpyrazine, 2,6-methylpyrazine, 2,5-methylpyrazine, and trimethylpyrazine in the xylose–glycine Maillard reaction model was researched. Ellagic acid could either inhibit or promote pyrazine formation, depending on its addition time point and the pH of the system. The addition of ellagic acid during the accumulation period of an Amadori compound inhibited pyrazine formation by capturing the Amadori compound in the xylose–glycine Maillard system and decreasing the pyrazine precursors. The inhibitory effect of ellagic acid on pyrazine formation got more obvious with an increase in the pH of the system. However, when ellagic acid was added at the beginning of the xylose and glycine Maillard system and when the oxidizing substances such as glyoxal and methylglyoxal were significantly formed in the Maillard system, its oxidation could promote the formation of pyrazines.
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BIOMACROMOLECULES
·
2022
·
IF 6.978
Yu Wang,Shuang Zeng,Hongyan Cui,Haidong Li,Zhen Li,Jingyun Wang,Qixian Chen
Proteins have been perceived as being an intriguing modality of therapeutics for the treatment of intractable diseases in view of their superlative precision and versatility. Nonetheless, proteins’ intrinsic characters, particularly their being hydrophilic macromolecules with unmethodical charges, have imposed the exceeding challenge of seeking transcellular trafficking into cells’ interiors. To circumvent this drawback, we have attempted to employ triple-functional amine-reactive 4-(2-((2-(((4-nitrophenoxy)carbonyl)oxy)ethyl)disulfaneyl)ethoxy)-4-oxobutanoic acid for the efficient incorporation of the anionic carboxyl moiety into amine-enriched enzymes, resulting in overall negatively charged pro-enzymes. The resulting pro-enzymes could be readily electrostatically assembled with cationic species [for instance: block copolymers of poly(ethylene glycol)-polylysine] into core–shell architectural delivery nanoparticles for their facilitated endocytosis into cells. Noteworthy is the aforementioned carboxylation chemistry designed to allow facile reversal of the pro-enzymes to the original amine groups due to the thiolysis of intermediate disulfide linkage for subsequent cascade reactions in response to the cytosol-enriched glutathione. Therefore, cytosol-selective structural disassembly for the liberation and activation of the pro-enzymes was accomplished. Our subsequent investigations utilizing ribonuclease A and catalase as the model enzymes demonstrated appreciable transcellular transportation of the active enzymes to the cell interiors, exerting overwhelming cytotoxic potencies and H2O2 scavenging capacities, respectively. Hence, we reported an unprecedented redox-stimulated charge reversal strategy in engineering cytosol-activatable pro-enzymes, manifesting a simple and efficient approach in the manufacture of transcellular proteinic therapeutics, which should be highlighted to promote their wide availability for use with diverse functional proteins as molecular biological tools and precision therapeutics.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Xue Xia,Yun Zhai,Heping Cui,Han Zhang,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
The Amadori compound of glucose and l -alanyl- l -glutamine (Ala-Gln-ARP) was prepared and characterized by UPLC-MS/MS and NMR. There were no pyrazines produced by heated Ala-Gln-ARP alone due to the asynchronicity of regenerated l -alanyl- l -glutamine and α -dicarbonyl compounds. High temperature (130 °C) and long reaction time could facilitate the 2,5-dimethylpyrazine formation at a small concentration (33.4 ± 3.47 μg/L). The exogenous amino substrates would lower the formation temperature of pyrazines and make it to be generated effectively. Extra supplied l -alanyl- l -glutamine could generate 2,5-dimethylpyrazine at 110 °C, while higher temperature of 140 °C could strengthen the formation of 2,5-dimethylpyrazine (793 ± 119 μg/L) and stimulate the generation of other pyrazines, including methylpyrazine and 2,6-dimethylpyrazine. The exogenous alanine, glutamic acid, and glutamine was also beneficial to enhance the pyrazines formation, especially the glutamic acid. Furthermore, alkaline pH of thermal reaction made pyrazines increase significantly than in neutral medium and further enriched their species such as unsubstituted pyrazine and trimethylpyrazine.
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Foods
·
2022
·Vol. 11
·期 9
·1178
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IF 5.561
Xiaoqi Li,Shuyuan Du,Fangyuan Tian,Minglu Wang,Ying Wang,Hongyan Zhang,Liguo Zang
The residue of estrogenic-disrupting compounds (EDCs) that are secreted by cows, added as drugs, and present in the feed may exist in dairy products. A gold nanoparticles (AuNPs)-estrogen receptor (ER) cocktail colorimetric assay equipped with ER cocktail solid phase extraction (SPE) was established to screen EDCs. Nine EDCs with high, moderate, and low estrogenic activity were selected to be the representative targets. The recognition range of the colorimetric assay combined with the ER cocktail SPE was wider than that of a single ERα or ERβ. The lowest detection limit of the established assay was about 10−9mg·mL−1. The detection limits of estrone, bisphenol A, and bisphenol B were about one order of magnitude lower than the method based on a single ER. The recoveries of the spiked nine EDCs were between 80.0% and 110.0%, and daidzein was identified in the dairy product. The developed method has potential application prospects in food safety and environmental monitoring.Keywords:estrogenic-disrupting compounds;estrogen receptor cocktail;solid phase extraction;gold nanoparticles colorimetric assay;dairy products
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Catalysis Science & Technology
·
2022
·Vol. 12
·期 9
·2846-2855
·
IF 6.177
Amjad Hussain Memon,Bin Wei,Saira Shams,Yucui Jiang,Mengzhao Jiao,Mingming Su,Hao Liang
The growing proportion of the textile industry has led to an increase in the concentration of colored dyes in aquatic systems. Compared to traditional natural enzymes, enzyme-mimicking nanomaterials with higher stability and lower sensitivity have become a promising approach for dye removal. In this work, a simple and efficient method was described to synthesize hematin nanocomposites (hematin@Cu/GMP) for degradation of several dyes, such as orange G (OG), amido black 10B (AB), crystal violet (CV), patent blue VF (PB) and methyl orange (MO). The experimental data suggested that the hematin-based nanocomposite exhibited excellent binding affinity towards Cu2+ and GMP with no effect on the composite formation in the presence of chloride salts. Further, the bi-enzymatic activity of the discussed composite was evaluated against different substrates with performance relatively better than natural laccase and horseradish peroxidase. The catalytic activity of the hematin@Cu/GMP demonstrated excellent potential with 100% degradation efficiency for the said dyes. The composite furthermore demonstrated relatively better performance for the degradation of the OG dye with retained catalytic activity under harsh chemical conditions. Moreover, the composite demonstrated excellent reusability and stability against inorganic radicals including cations, anions, and other organic solvents. The bi-enzymatic activity and catalytic potential of the proposed nanocomposites are suggestive of their reliability for greener industrial applications.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Xuwen Gao,Huimin Zhao,Dongyang Wang,Yuqi Xu,Bin Zhang,Guizheng Zou
Singlet oxygen (1O2) is an important reactive oxygen species (ROS) that is intensively involved in natural photochemical and photobiological processes. Herein, selectively lighting up 1O2 is achieved in the aggregation-induced emission (AIE) of electrochemiluminescence (ECL) from the Zn2+-mediated AIE assembly of Au nanoclusters (Zn2+-AIE-AuNCs). Zn2+-AIE-AuNCs can exhibit efficient AIE ECL and photoluminescence (PL) along with 1O2 generation in energy and charge transfer routes, respectively. The AIE ECL of the Zn2+-AIE-AuNCs/tripropylamine (TEA) system in carbonate buffer is located around 703 nm with the dimeric aggregate of 1O2 as an emitter because electrochemically oxidizing coexisted Zn2+-AIE-AuNCs and TEA in carbonate buffer would promote the oxygen vacancy (Ov) of Zn2+-AIE-AuNCs, which could selectively enable the generation of emissive singlet oxygen in the energy transfer route by effectively transferring the energy from excited singlet Zn2+-AIE-AuNCs to the triplet ground state of dissolved oxygen (3O2). No emissive 1O2 is detected via electrochemically oxidizing the Zn2+-AIE-AuNCs in the case without either carbonate buffer or TEA, and the Zn2+-AIE-AuNCs/TEA system can only exhibit AIE ECL around 485 nm with Zn2+-AIE-AuNCs as the emitter in carbonate-free buffers. Photoexciting Zn2+-AIE-AuNCs merely brings out band-gap-engineered AIE PL around ∼485 nm with Zn2+-AIE-AuNCs as the emitter, which manifests that the 1O2 generated in the charge transfer route via photoexciting Zn2+-AIE-AuNCs is un-emissive. This work not only proposes an effective strategy for AIE with 1O2 as an emitter but also opens a promising way to selectively light up 1O2.
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ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Qing Hu,Jiayi Yao,Xiaoqin Wang,Yanfang Wang,Xiaoling Fu,Ju Ma,Han Lin,Jiaqi Xu,Longhua Shen,Xiangbin Yu
Chemoimmunotherapy can synergistically enhance the therapeutic effects and decrease the side effects by a combined method. However, the effective targeted codelivery of various chemotherapeutic agents and siRNAs remains challenging. Although nanomedicine-based chemoimmunotherapy has shown great potential in cancer treatment in recent years, further effort is needed to simplify the nanocarrier designs and maintain their effective functions. Here, we report a simple but robust multifunctional liposomal nanocarrier that contains a pH-sensitive liposome (LP) shell and a dendritic core for tumor-targeted codelivery of programmed cell death ligand 1 (PD-L1) siRNA and doxorubicin (DOX) (siPD-L1@PM/DOX/LPs). siPD-L1@PM/DOX/LPs had a suitable particle size and zeta potential, excellent stability in serum, and pH-sensitive drug release in vitro. They exhibited significant cell proliferation inhibition compared to free DOX and DOX-loaded LPs and could escape endosomes, effectively release siRNA into the cytoplasm of MCF-7 cells, and significantly reduce the PD-L1 expression on tumor cells. In vivo imaging confirmed high accumulation of siPD-L1@PM/DOX/LPs at the tumor site. More importantly, compared with siPD-L1@PM/LPs or DOX alone, siPD-L1@PM/DOX/LPs were more effective in inhibiting tumor growth and activating cytotoxic T cells in vivo. In conclusion, this nanocarrier may hold promise as a codelivery nanoplatform to improve the treatment of various solid tumors.
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Bioactive Materials
·
2022
·
IF 16.874
Jianhua He,Wenli Zhang,Xiaoju Zhou,Fengfei Xu,Jiahui Zou,Qiqi Zhang,Yi Zhao,Hongliang He,Hu Yang,Jianping Liu
Nanoparticle-based therapeutics represent potential strategies for treating atherosclerosis; however, the complex plaque microenvironment poses a barrier for nanoparticles to target the dysfunctional cells. Here, we report reactive oxygen species (ROS)-responsive and size-reducible nanoassemblies, formed by multivalent host-guest interactions between β-cyclodextrins (β-CD)-anchored discoidal recombinant high-density lipoprotein (NP 3 ST ) and hyaluronic acid-ferrocene (HA-Fc) conjugates. The HA-Fc/NP 3 ST nanoassemblies have extended blood circulation time, specifically accumulate in atherosclerotic plaque mediated by the HA receptors CD44 highly expressed in injured endothelium, rapidly disassemble in response to excess ROS in the intimal and release smaller NP 3 ST , allowing for further plaque penetration, macrophage-targeted cholesterol efflux and drug delivery. In vivo pharmacodynamicses in atherosclerotic mice shows that HA-Fc/NP 3 ST reduces plaque size by 53%, plaque lipid deposition by 63%, plaque macrophage content by 62% and local inflammatory factor level by 64% compared to the saline group. Meanwhile, HA-Fc/NP 3 ST alleviates systemic inflammation characterized by reduced serum inflammatory factor levels. Collectively, HA-Fc/NP 3 ST nanoassemblies with ROS-responsive and size-reducible properties exhibit a deeper penetration in atherosclerotic plaque and enhanced macrophage targeting ability, thus exerting effective cholesterol efflux and drug delivery for atherosclerosis therapy.
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INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Liu Mengfan,Yao Jingjing,Zhang Chengliang,Hao Runqin,Zhang Zhongguo,Cao Wenbo
Wood vinegar is a complex mixture of organic compounds. QAMS is a relatively simple and cost-effective method which is used widely in recent years. In this study, quantitative analysis of multi-components by single-marker (QAMS) was applied to quantify the main volatile chemical components in wood vinegar for the first time. Using acetic acid as an internal reference compound, the relative correction factors of a further 19 chemical volatile components were obtained. 20 wood vinegar prepared from different lignocellulosic biomass, and at different pyrolysis temperatures, were analyzed by QAMS and the data compared with those obtained using an internal standard (phenethyl acetate) method. The results showed that the precision and repeatability of the QAMS method were good, and the responses obtained for each compound using GC/MS were linear over the corresponding concentration range (R 2 ≥ 0.999). The average spike recoveries of 20 volatile compounds from wood vinegar ranged from 92.65% to 105.93% (RSD ＜ 7%, n = 6). There were no significant differences (P > 0.05) in the results obtained from the analysis of three batches of wood vinegar samples (RSD < 2%) using both methods. The volatile composition of samples obtained from wood vinegar prepared from the pyrolysis of different materials and at different temperatures were significantly different. The successful application of QAMS to the quantitative analysis of the composition of wood vinegar not only may provide a theoretical reference for further developments in the production, pyrolysis conditions, and modes of action for wood vinegar, but also provides ideas for the application of QAMS in other fields.
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ANALYTICA CHIMICA ACTA
·
2022
·
IF 6.911
Yu Liu,Yanan Cao,Cong Zhang,Cheng Ye,Qinglai Bian,Xue Cheng,Hui Xia,Junping Zheng,Hongtao Liu
The inhibition of hydrogen peroxide (H 2 O 2 ) on the cysteine-mediated aggregation of gold nanoparticles (AuNPs) has great potential to rapidly visualize H 2 O 2 and disease-associated biomarkers. However, associated applications have been rigid due to the low cysteine-oxidation efficiency or the insufficient interference-resistance of previous catalysts. Here, we proposed a novel AuNPs colorimetric method termed Fe 2+ -catalyzed H 2 O 2 -preventing aggregation of AuNPs by oxidizing Cysteine (FeHOAuC) for the visible and rapid detection of H 2 O 2 with high specificity. Formed Fe 2+ -cysteine composites in the beginning, Fe 2+ accelerates the oxidation of cysteine by H 2 O 2 with an intramolecular electron-transferring model, and the generated cysteine oxides (including cysteic acid or cystine , dependent on the ratio of cysteine to H 2 O 2 molecules) lose the pro-aggregation effect on AuNPs. Notably, FeHOAuC can quantify 2–30 μM of H 2 O 2 within 5 min, and the monitoring process works well with tolerance to the impact of dissolved oxygen. The FeHOAuC paired with lactate oxidase was successfully used for measuring lactic acids in real sweat samples with a practicable detection window (2–60 μM) and a low variable coefficient (<10%). In summary, FeHOAuC is a feasible platform for rapid and convenient detection of H 2 O 2 and oxidase-specific substrates.
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Biology-Basel
·
2022
·Vol. 11
·期 4
·503
·
IF 5.168
Jingyun Ding,Huiyan Wang,Zequn Li,Jiangnan Sun,Peng Ding,Xiaomei Chi,Mingfang Yang,Yaqing Chang,Chong Zhao
Simple SummarySea urchins are key in the trophic cascades of benthic communities. They also possess physiological characteristics of low digestibility. Thus, we explored the relationship between the indicators (digestive enzymes activities and the gut emptying) and molecules (TRPA1 and 5-HT) related to digestive physiology in the sea urchinStrongylocentrotus intermedius. The results in the present study indicate that digestive enzyme activities and gut emptying are correlated with the TRPA1 and 5-HT in the gut ofS. intermedius.This novel finding not only is important for understanding the low food digestibility of sea urchins but provides an entry point to further investigate the molecular details of digestive physiology of sea urchins.AbstractThe energetic link in the benthic community is based on physiological characteristics of the low food absorption efficiency of sea urchins. Low food absorption efficiency of sea urchins is correlated with the activity of digestive enzymes and the duration of food in their gut. Thus, the digestive enzymes activities (pepsin and amylase enzyme activities) and gut emptying are important indicators in assessing nutrient digestion and absorption in sea urchins. In the present study, the relationship between these indicators and molecules related to digestive physiology were quantified in sea urchins. We found (1) an inter-regulatory relationship existed between Transient receptor potential cation channel, subfamily A, member 1 (TRPA1), and serotonin (5-hydroxytryptamine; 5-HT) in the gut ofStrongylocentrotus intermedius; (2) digestive enzyme activities were negatively correlated with the TRPA1 and concentration of 5-HT in the gut ofS. intermedius; (3) gut emptying rate was positively correlated with TRPA1 and concentration of 5-HT in the gut ofS. intermedius. The present study revealed that the digestion and absorption of food are correlated with the TRPA1 and 5-HT in the gut ofS. intermedius, which provides valuable information about the digestive physiology of sea urchins. This novel finding is relevant to understanding the low food digestibility of sea urchins. It also provides valuable information to the digestive physiology of sea urchins, which are key to maintaining the stability of food webs in the marine ecosystem.Keywords:sea urchin;TRPA1;5-HT;digestive enzyme;gut emptying
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危险品化学品经营许可证（带存储）