Frontiers in Pharmacology
·
2024
·
IF 4.4
Shu Long,Fu Hangjie,Pi Aiwen,Feng Yuliang,Dong Hui,Si Caijuan,Li Songtao,Zhu Feiye,Zheng Peifen,Zhu Qin
Ulcerative colitis (UC) is a recurring inflammatory bowel disease, in which oxidative stress plays a role in its progression, and regulation of the oxidative/antioxidative balance has been suggested as a potential target for the treatment of UC. The aim of this study was to evaluate the protective effect of andrographolide against UC and its potential antioxidant properties by modulating the nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway. Dextran sulfate sodium (DSS) -induced UC mice and the LPS-induced HT29 inflammatory cell model were established to uncover the potential mechanisms of andrographolide. ML385, a Nrf2 inhibitor, was used in both models to assess whether andrographolide exerts a protective effect against UC through the Nrf2/HO-1 pathway. The in vivo experiment showed that andrographolide ameliorated the symptoms and histopathology of DSS-induced mice and restored the expressions of ZO-1, Occludin-1 and Claudin-1. Meanwhile, DSS-induced oxidative stress and inflammation were suppressed by andrographolide treatment, along with the upregulation of key proteins in the Nrf2/HO-1 pathway. In vitro experiments showed that andrographolide attenuated LPS-induced excessive generation of ROS in HT29 cells, reduced inflammatory factors, and upregulated the expression of proteins related to tight junctions and Nrf2/HO-1 pathway. In addition, ML385 abolished the beneficial effect of andrographolide. In conclusion, the protective effect of andrographolide against UC may involve the suppression of oxidative stress and inflammation via the Nrf2/HO-1 pathway.
✓ 已验证
DOI
PubMed
高影响因子
MOLECULAR NUTRITION & FOOD RESEARCH
·
2022
·Vol. 66
·期 23
·2200300
·
IF 6.575
Zhaodi Zhang,Jiahong Li,Ming Zhang,Binbin Li,XiaoHua Pan,Xiaoliang Dong,Li-Long Pan,Jia Sun
Scope Dietary fibers can alter gut microbiota and microbial metabolite profiles. SCFAs are produced by bacterial fermentation of fiber, mediating immune homeostasis through G-protein-coupled receptors (GPCRs). GPR109a, a receptor for niacin and butyrate, expressed by immune cells and non-immune cells, is a key factor regulating immune responses. However, the role and underlying mechanisms of GPR109a in type 1 diabetes (T1D) remain unclear. Methods and results Experimental T1D was induced by streptozotocin in GPR109a-deficient ( Gpr109a −/− ) and wild type mice. The study found that Gpr109a −/− mice were more susceptible to T1D with dysregulated immune responses, along with increased M1 macrophage polarization (from 10.55% to 21.48%). Further, an adoptive transfer experiment demonstrated that GPR109a-deficient macrophages promoted the homing of intestine-derived type 1 cytotoxic T cells to pancreas (from 18.91% to 24.24%), thus disturbing the pancreatic immune homeostasis in non-obese diabetic mice. Mechanistically, GPR109a deficiency promoted M1 macrophage polarization associated with the activation of suppressor of cytokine signaling 3-signal transducer and activator of transcription 1 signaling pathway. Conclusion The findings reveal that macrophage GPR109a deficiency accelerates the development of T1D. Activation of GPR109a on macrophage by dietary components may provide a new strategy for preventing or treating T1D.
✓ 已验证
DOI
PubMed
高影响因子
ANNALS OF CLINICAL AND LABORATORY SCIENCE
·
2023
·Vol. 53
·期 1
·116-125
·
IF 0.8
Yunfang Cai,Ting Zhang,Guiying Chen,Chunxi Liu
Objective Flap endonuclease 1 (FEN1) has been confirmed to involve the drug resistance of multiple cancers including breast cancer.However, the effect of miRNA-mediated FEN1 on breast cancer cell resistance is still ambiguous and needs further research. Methods Firstly, we used GEPIA2 to predict the FEN1 expression in breast cancer. Next, we used quantitative real-time polymerasechain reaction (qRT-PCR) and western blot to evaluate the FEN1 level of cells. After parental cells or MDA-MB-231-paclitaxel(PTX) cells being transfected with or without siFEN1, the apoptosis, migration, and protein levels of FEN1, Bcl-2, and resistance-relatedgenes were examined by flow cytometry, wound healing assay, and western blot, respectively. Then, the putative miRNA targetingFEN1 was predicted using StarBase V3.0, and further confirmed by qRT-PCR. The targeted binding of FEN1 to miR-26a-5p was detectedby dual-luciferase reporter assay. After parental cells or MDA-MB-231-PTX cells being transfected with or without miR-26a-5pmimic, the apoptosis, migration, and protein levels of FEN1, Bcl-2, and resistance-related genes were tested again. Results FEN1 expression was enhanced in breast cancer and MDA-MB-231-PTX cells. The combined application of FEN1 knockdown and PTXenhanced apoptosis in MDA-MB-231-PTX cells but suppressed cell migration and expressions of FEN1, Bcl-2, and resistance-relatedgenes. Then, we confirmed that FEN1 was targeted by miR-26a-5p. The combined application of miR-26a-5p mimic and PTX largelyfacilitated apoptosis in MDA-MB-231-PTX cells but restrained cell migration and expressions of FEN1, Bcl-2, and resistance-relatedgenes. Conclusion MiR-26a-5p contributes to the sensitivity of breast cancer cells to paclitaxel via restraining FEN1.
✓ 已验证
DOI
PubMed
高影响因子
Scientific Reports
·
2025
·
IF 3.9
Xu Qiuling,Liu Zhuoxi,Wang Liyuan,Liu Guoyang,Zhao Long,Yue Hui,Liu Yong
Diabetic cardiomyopathy (DCM) requires a systematic approach for effective management. Focusing on key signaling pathways in myocardial fibrosis could offer novel therapeutic approaches for DCM. The objective of this research is to investigate new biomarker and the underlying mechanisms by which baicalin intervenes in the progression of DCM. Swiss Target Prediction databases and weighted correlation network analysis (WGCNA) were employed to collect and analyze targets of baicalin and DCM. Intersection targets were obtained through venn online website, network visualization was conducted through STRING database and Cytoscape 3.7.2 software. Molecular docking validation was completed via AutoDock Vina software. Western blotting, RT-qPCR and histology were carried out to validate the direct targets of baicalin and explore the molecular mechanism during DCM progression. In mice induced to DCM, we observed that disruptor of telomeric silencing 1-like (DOT1L) was upregulated. Baicalin (BC) reduced myocardial fibrosis in mice as indicated by the reduced expression levels of fibrosis markers COL-1 and TGF-β. The inhibitory effect of BC was reversed by overexpressed DOT1L and further enhanced by silenced DOT1L. Our results indicate that cardiomyocyte DOT1L exerts a pathogenic role in DCM and BC specifically targets DOT1L/COL-1 signaling to reduce myocardial fibrosis in DCM.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
·
2023
·
IF 5.2
Pei Li,Zhishuang Yang,Ting Lei,Yujie Dai,Yang Zhou,Dekang Zhu,Hongyan Luo
Objectives: To elucidate the role of a novel carbapenem-hydrolysing class D β-lactamase (RAD-1) from Riemerella anatipestifer. Methods: We applied WGS and bioinformatic analysis to screen putative β-lactamase genes in R. anatipestifer SCVM0004. A putative class D β-lactamase gene was cloned into pET24a and transferred into Escherichia coli BL21 (DE3) for antibiotic susceptibility determination and protein purification. Meanwhile, the purified native protein was used to determine the enzymatic activities. Results: A class D β-lactamase, RAD-1, was identified in the genome of R. anatipestifer SCVM0004. It was distinct from all characterized class D β-lactamases (≤42% amino acid sequence identity). Searching in GenBank showed that blaRAD-1 was widely disseminated among R. anatipestifer. Genomic environment analysis indicated that the chromosomal structures of blaRAD-1-located regions were relatively conserved. Expression of RAD-1 in E. coli results in elevated MICs for various β-lactam antibiotics, including penicillins, extended-spectrum cephalosporins, a monobactam and carbapenems. Moreover, kinetic analysis of purified RAD-1 revealed: (i) high-level activity against penicillins; (ii) highest affinity for carbapenems; (iii) moderate hydrolysis of extended-spectrum cephalosporins and a monobactam; and (iv) no activity for oxacillin and cefoxitin. Conclusions: This study identified a novel chromosomally located class D carbapenemase RAD-1 (Bush-Jacoby functional group 2def) in R. anatipestifer SCVM0004. Moreover, bioinformatic analysis confirmed that the RAD-1 was widely prevalent and conserved in R. anatipestifer.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Hui Gu,Dong Chen,Jia-Ping Huang,Yu-Ting Wu,Lu Hu,Shao-Liang Jiang,Jie-Hua Shi
Entrectinib (ENB) is a multi-target tyrosine kinase inhibitor currently approved for the treatment of adult and pediatric patients with NTRK fusion-positive advanced recurrent solid tumors and metastatic ROS1-mutated non-small cell lung cancer. The interaction between ENB and human α1-acid glycoprotein (HAG) was assessed by multispectral experiments and computer simulations. The results demonstrated that ENB effectively quenched HAG fluorescence via a mixed quenching mechanism. Specifically, ENB bound to the hydrophobic cavity of HAG, forming an ENB-HAG complex with 1:1 stoichiometry. The binding constant (∼10 6 M −1 ) demonstrated strong binding affinity between ENB and HAG. Non-radiative energy transfer occurred between ENB and HAG, with a binding distance of 2–4 nm. Synchronous and three-dimensional fluorescence spectroscopy indicated minimal effects on the microenvironment around the TYR and TRP residues. Circular dichroism spectroscopy demonstrated a slight alteration on the conformation of HAG, specifically a reduction in β-folded content from 43.5 % to 43.3 %. Thermodynamic calculations confirmed spontaneous binding driven primarily by hydrophobic force, which was further supported by Molecular dynamics simulationsanalyzing energy contributions of individual amino acid residues. Additionally, metal ions were found to exert varying degrees of influence on the binding process.
✓ 已验证
DOI
高影响因子
Food & Function
·
2018
·Vol. 9
·期 9
·5007-5015
·
IF 3.289
Zheng-Nan Cai,Wan Li,Shomaila Mehmood,Wen-Juan Pan,Qing-Xi Wu,Yan Chen,Yong-Ming Lu
Polysaccharides from Morchella esculenta are known to exhibit diverse bioactivities, while an anti-melanogenesis effect has been barely addressed. Herein, the anti-melanogenesis activity of a heteropolysaccharide from M. esculenta (FMP-1) was investigated in vitro and in vivo. FMP-1 had no significant cytotoxic effect on B16F10 melanoma cells as well as zebrafish larvae, but did reduce melanin contents and tyrosinase activities in both of them. Treatment with FMP-1 also effectively suppressed the expression of melanogenesis-related proteins, including MC1R, MITF, TRP-1 and TRP-2, through decreasing the phosphorylation of cyclic adenosine monophosphate response element-binding protein (CREB). Moreover, the mitogen-activated protein kinase (MAPK) pathway was observed mediating FMP-1's inhibitory effect against melanin production. Specifically, FMP-1 treatment markedly inhibited the activation of phosphorylation of p38 mitogen-activated protein kinase. These results suggested that FMP-1's inhibitory effect against melanogenesis is mediated by the inhibition of CREB and p38 signaling pathways, thereby resulting in the downstream repression of melanogenesis-related proteins and the subsequent melanin production. These data provide insight into FMP-1's potential anti-melanogenesis effect in food and cosmetic industries.
✓ 已验证
DOI
PubMed
高影响因子
Materials Science and Engineering B-Advanced Functional Solid-State Materials
·
2025
·
IF 4.6
Huiping Wu,Hui Sun,Bin Yu,Afang Gao,Gaoyuan Chen,Xinyu Liu,Shijie Wang,Yutong Feng
Recently, the rapid growth of the textile, printing, and dyeing industries has led to severe pollution from dye wastewater. Bimetallic metal-organic frameworks (bimetallic MOFs) have emerged as promising adsorbents for dyes removal. Herein, Fe-HKUST-1 with an octahedral structure was anchored on the surface of viscose spunlaced nonwoven material (VSN) via an in situ solvothermal method to prepare Fe-HKUST-1@VSN. After carbonization, Fe-HKUST-1@VSNC was obtained for the adsorption of organic dyes in water. When the carbonization temperature is no more than 240 ο C, the morphologies and structures of Fe-HKUST-1@VSNC are not changed. And the adsorption efficiencies and capacities of Fe-HKUST-1@VSNC-240 for Congo red (CR) and crystal violet (CV) are the optimal. At an initial dye concentration of 10 mg L −1 , a temperature of 35 °C, and pH 7, Fe-HKUST-1@VSNC-240 shows its highest performance, reaching 98.17 % removal of CV in 90 min and 98.65 % removal of CR in 120 min. The adsorption processes of Fe-HKUST-1@VSNC-240 for both CV and CR are slightly affected by coexisting ions. The adsorption efficiency of Fe-HKUST-1@VSNC-240 for CV and CR declines to 50.10 % (after 6 cycles) and 56.76 % (after 22 cycles), respectively. Moreover, Fe-HKUST-1@VSNC-240 also demonstrates good adsorption performance in real water environment. The adsorption processes of Fe-HKUST-1@VSNC-240 for both CV and CR are endothermic, spontaneous, and monolayer adsorption, primarily through chemical adsorption and intra-particle diffusion. The synthesized Fe-HKUST-1@VSNC-240 can adsorb various organic dyes, along with excellent structural stability and recyclability, offering a promising solution for dye-contaminated wastewater remediation.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LOSS PREVENTION IN THE PROCESS INDUSTRIES
·
2025
·
IF 4.2
Junjie Gu,Xinglin Wen,Yong Pan,Lei Ni
1-methyl-2,4-cyclohexanediamine (2,4-MCHD) is the core raw material for the synthesis of high-grade polyurethane. Aiming at the problems of long reaction time and safety risks in batch reactors, a fast and safe synthesis scheme of 2,4-MCHD was proposed. The one-pot catalytic hydrogenation of 2, 4-dinitrotoluene (2, 4-DNT) to 2, 4-MCHD was investigated in a microfilled bed reactor (μPBR) over the 5%LiOH-5%Ru/γ-Al 2 O 3 catalyst. The effects of temperature, pressure, gas and liquid volumetric were investigated. Under the optimized condition (180 °C, 7 MPa H 2 pressure, 0.6 mL/min liquid flow rate, 40 mL/min gas flow rate), within a residence time of 144 s, the conversion of 2,4-DNT and the selectivity of 2,4-MCHD exceeded 99% and 80%, respectively. Compared to the conventional batch mode, an increase of one to two orders of magnitude in space-time-yield (STY) was realized under continuous flow mode. Furthermore, the inherent risks of high-pressure hydrogenation in batch processes are significantly mitigated in the μPBR due to its minimal hydrogen inventory and superior heat and mass transfer characteristics.
✓ 已验证
DOI
高影响因子
Journal of Superconductivity and Novel Magnetism
·
2025
·Vol. 38
·期 2
·112
·
IF 1.7
Li Xiaofeng,Gao Yuxia,Tian Zhaoming,Ren Weijie,Sun Xin,Wang Mingli
KNi(PO 3 ) 3 was successfully synthesized by solid-state method and the magnetic properties were characterized. The crystal structure of KNi(PO 3 ) 3 belongs to trigonal crystal system with space group R 3, where Ni 2+ ions form the distorted honeycomb-lattice within the ab-plane stacking in the “ABAB” type fashion along the c -axis. Magnetization measurements reveal the presence of antiferromagnetic (AFM) interaction with Curie Weiss (CW) temperature θ CW = − 12.905 K but without long-range magnetic order down to 2 K. Notably, the CW fitted effective moment µ eff = 3.35µ B and saturated magnetization M S = 2 μ B /Ni 2+ at 2 K support KNi(PO 3 ) 3 as an S = 1 spin system. Intriguingly, the zero-field specific heat exhibits a broad peak maximized at ~ 2.5 K indicating the onset of short-range spin correlation between Ni 2+ ions, while the integrated magnetic entropy ( S mag ) is close to the expected Rln3 for S = 1 system, indicative of large spin fluctuation below 2 K driven by spin frustration.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS CHEMISTRY
·
2012
·Vol. 22
·期 18
·8759-8763
·
IF 5.968
Yuhan Lin,Fang Zhang,Daocheng Pan,Hexing Li,Yunfeng Lu
TiO2/(ZnS)x(CuInS2)1−x nanocomposites were prepared from oleic acid-capped TiO2 nanocrystals and alloyed (ZnS)x(CuInS2)1−x nanocrystals. Element mapping and transmission electron microscopy (TEM) results suggested that the nanocomposites exhibit homogeneous composition with uniform mesoporous structure. High photocatalytic activities driven by simulated solar light were achieved owning to their high surface area and efficient electron transfer from (ZnS)x(CuInS2)1−x to TiO2. This work enables the synthesis of a large variety of photocatalysts from nanocrystal building blocks for various applications beyond photodegradation of organic pollutants.
✓ 已验证
DOI
高影响因子
ACS Applied Nano Materials
·
2021
·
IF 5.097
Ying Zheng,Mingqiang Cheng,Xinyan Wu,Shuang Zhang,Zhu Liu,Yanqiang Li,Weiquan Shao,Qing Lin,Jinshan Tan,Shouwu Gao,Yongcheng Zhang,Wanneng Ye
Mechanical energy can be directly transformed into chemical energy by piezoelectric materials, namely, piezocatalysis, which is a potential tactic for renewable clean energy collection and environmental purification. The piezocatalytic efficiency strongly relies on the piezoelectric property and free charge concentration of piezocatalysts. Here, four samarium-doped (1 – x)Pb(Mg1/3Nb2/3)O3-xPbTiO3 nanostructures were designed to investigate the correlation between the piezoelectric property and piezocatalytic performance. The diverse dye degradation efficiencies confirmed the high piezoelectric coefficient toward high catalytic activity. The degradation efficiency for acid orange 7 (AO7) was 100% within 20 min, while those for methyl orange (MO), methylene blue, and rhodamine B dyes were 97, 78, and 72%, respectively, within 40 min under ultrasonic vibration only. Furthermore, high catalytic efficiency of 96% was still maintained for AO7 degradation after 10 consecutive degradation cycles for samarium-doped 0.70Pb(Mg1/3Nb2/3)O3-0.30PbTiO3 due to its high piezoelectric coefficient. The investigation of the catalytic mechanism demonstrated that hole and superoxide radicals were the primary active species toward AO7 degradation, whereas hydroxyl and hole radicals were for MO degradation. This work not only demonstrates the highly efficient catalytic activity of samarium-doped (1 – x)Pb(Mg1/3Nb2/3)O3-xPbTiO3 nanostructures but also gives a deep comprehension of the correlation between the piezoelectric property and catalytic performance of piezocatalysts.
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）