INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yun Liu,Zhina Qiao,Hengwei Zhang,Xian Zhang,Yanan Li,Zhiming Rao
D-tagatose, a functional rare sugar, has physiological effects such as lowering blood sugar and anti-caries. However, research on the industrial production of D-tagatose has primarily focused on Escherichia coli , limiting application in the food field. In this study, a cell factory for efficient synthesis of D-tagatose was constructed using the GRAS strain Corynebacterium glutamicum . Firstly, the key enzyme L-arabinose isomerase from Lactobacillus fermentum was screened. Secondly, by rational design, the optimal mutant D390V/H339P significantly enhanced enzyme activity by 40.65 % and improved thermostability. Subsequently, a dual-enzyme coupled whole-cell system was established by regulating the ratio of β-galactosidase and L-arabinose isomerase by RBS optimization. After optimizing the catalytic system, 101.42 g/L D-tagatose was produced from 400 g/L lactose, and the conversion rate reached 25.12 % in 5 L fermenter, which is the highest yield using lactose as substrate and GRAS strain as chassis cells. This research laid a foundation for the industrial application of food-grade D-tagatose.
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高影响因子
NUTRITIONAL NEUROSCIENCE
·
2025
·
IF 3.6
Jiahao Liu,Jiawei Li,Yuanyuan Liu,Ruici Liu,Liya Fang,Zhimei Jiang,Lei Liu,Luchuan Wang,Chao Gong,Deming Kong,Ruting Fu,Pei Zeng,Jin Guo,Shaobo Zhou
Objective Epilepsy treatments often lead to vitamin D (VitD) deficiency. Although vitamin D₃ (VitD3) has been shown to reduce epileptic symptoms by 43%, its preventive effects remain unclear. This study investigated the potential of VitD pretreatment in two common acute epilepsy mouse models and explored its effects on seizure severity, latency, and molecular mechanisms involving calcium-sensing receptor (CaSR), phosphatase and tensin homolog (PTEN), and autophagy.Methods Mice were randomly divided into nine groups (n = 15). VitD or vehicle was administered 40 min before pentylenetetrazole (PTZ) or kainic acid (KA) given intraperitoneal injection (i.p). Seizure behavior and electroencephalograms (EEGs) were recorded for 60 min. After 24 h, hippocampal tissues were analyzed histologically and assessed for expression of autophagy-related proteins, CaSR, and PTEN.Results Both PTZ and KA induced acute seizures (Racine Grade IV+), with corresponding high-amplitude EEG spikes, neuronal damage, and mossy fiber sprouting. CaSR and autophagy markers were upregulated, while PTEN was downregulated, especially in the KA group. VitD pretreatment reduced seizure frequency, prolonged latency, alleviated hippocampal damage, downregulated CaSR and autophagy markers, and upregulated PTEN. These effects were milder than those of valproate. Combined VitD and Oroxin B treatment further improved outcomes.Conclusions (1) PTZ-induced seizures increased CaSR and decreased PTEN, triggering autophagy and worsening symptoms. (2) KA-induced epilepsy caused more severe damage with stronger autophagy activation. (3) VitD pretreatment mitigated seizures by modulating CaSR, PTEN, and autophagy, showing greater efficacy in the PTZ model.
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高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Shaowei Zheng,Ya Gao,Houkang Cao,Kai Guo,Wenxiu Wang,Guiying Lu,Shasha Wang,Mingli Zhong,Bo Li,Kefeng Zhang,Riming Wei,Jianzhao Wu
Background Acute liver failure (ALF) is associated with ferroptosis and currently lacks specific drugs and therapies. Previous studies have shown the hepatoprotective activity of Sophoricoside (SOP) in diverse models of hepatic injury. However, the role of SOP in ALF caused by lipopolysaccharide (LPS)/D-galactosamine (D-GalN) is still unknown. Purpose To investigate the therapeutic effects of SOP on ALF and its underlying mechanisms of action. Methods In vivo , an ALF mouse model was established using LPS and D-GalN. RNA sequencing was used to identify differentially expressed genes, which were subsequently confirmed by Western blot and qRT-PCR. In vitro , erastin was used to induce ferroptosis in HepG2 cells. Subsequently, the cells were treated with SOP, the nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor ML385, and the glutathione peroxidase 4 (GPX4) inhibitor RSL3. Additionally, siRNA was utilized for Nrf2 knockdown. Measurements of biochemical markers, reactive oxygen species (ROS) levels, alterations in mitochondrial membrane potential, qRT-PCR, and Western blot analysis were used to confirm the effectiveness and mechanism of SOP. Finally, the interaction between SOP and Nrf2 was verified through molecular docking, surface plasmon resonance (SPR), and cellular thermal shift assay (CETSA). Results In vivo , experimental analysis revealed that SOP ameliorated liver injury, hepatocyte Fe 2+ accumulation, and oxidative stress. RNA sequencing analysis indicated that SOP reversed the abnormal expression of genes related to Nrf2 and the ferroptosis pathway. In vitro , SOP ameliorated erastin-induced oxidative stress, ROS accumulation, and mitochondrial membrane potential loss. After the application of pharmacological inhibitors and siRNA knockdown, the SOP’s inhibitory effect on ferroptosis was antagonized. Conclusion SOP alleviated ALF by activating the Nrf2/GPX4 signaling pathway, thereby mitigating oxidative stress and ferroptosis.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Yanan Sun,Xiangze Jia,Chin Ping Tan,Bin Zhang,Xiong Fu,Qiang Huang
The formation of inclusion complexes (ICs) between V-type starch and flavors is traditionally conducted in an aqueous system. In this study, limonene was solid encapsulated into V6-starch under ambient pressure (AP) and high hydrostatic pressure (HHP). The maximum loading capacity reached 639.0 mg/g after HHP treatment, and the highest encapsulation efficiency was 79.9 %. X-ray Diffraction (XRD) results showed that the ordered structure of V6-starch was ameliorated with limonene, which avoided the reduction of the space between adjacent helices within V6-starch generated by HHP treatment. Notably, HHP treatment may force molecular permeation of limonene from amorphous regions into inter-crystalline amorphous regions and crystalline regions as the Small-angle X-ray scattering (SAXS) patterns indicated, leading to better controlled-release behavior. Thermogravimetry analysis (TGA) revealed that the solid encapsulation of V-type starch improved the thermal stability of limonene. Further, the release kinetics study showed that a complex prepared with a mass ratio of 2:1 under HHP treatment sustainably released limonene over 96 h and exhibited a preferable antimicrobial effect, which could extend the shelf life of strawberries.
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高影响因子
Biomaterials Science
·
2019
·Vol. 8
·期 3
·830-836
·
IF 5.251
Chengjun Wang,Ying Qian
A novel photosensitizer carbazolyl-BODIPY (Cz-BODIPY) with an orthogonal donor–acceptor structure was developed for photodynamic therapy (PDT). The photosensitizer Cz-BODIPY showed strong singlet oxygen sensitizing capability (ΦΔ = 0.68 in MeOH), excellent water solubility in dilute solution, and high photostability. The photosensitizer Cz-BODIPY exhibited negligible dark cytotoxicity and high phototoxicity (IC50 0.45 μM). Cz-BODIPY could induce cell apoptosis upon light illumination. Three cell states including living cells, apoptotic cells, and dead cells in the PDT process of Cz-BODIPY were determined via the Hoechst 33342/PI dual staining assays. The ROS (reactive oxygen species) generation in living cells during the PDT process of Cz-BODIPY was captured by the ROS detector, dihydroethidium (DHE). The photosensitizer Cz-BODIPY could be assimilated by zebrafish to generate ROS and diminish the integrity of zebrafish tissue upon light illumination. Tumor cell growth could be inhibited by Cz-BODIPY upon light illumination. The photosensitizer Cz-BODIPY displayed potential in real PDT application.
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高影响因子
Carbon Neutrality
·
2025
·Vol. 4
·期 1
·1-9
·
IF 12.5
Yang Delong,Dai Jinhang,Du Ziting,Cao Qingya,Chen Gang,Qi Ting,Wang Weilu,Li Fukun
Since 3-acetylamino-5-acetylfuran (3A5AF) has been regarded as a promising nitrogen-containing synthon, the conversion of renewable chitin and its monomer N-acetyl-D-glucosamine (NAG) to 3A5AF is of significance. In this paper, we compared the catalytic activity of several Cl-containing compounds and found that LiCl exhibited superior performance. The effects of solvent, catalyst dosage, reaction temperature, and reaction time on the synthesis of 3A5AF were investigated. Under the optimized conditions, about 60% yields of 3A5AF were obtained in N-methyl-2-pyrrolidone (NMP) or dimethylacetamide (DMA) medium. The spent LiCl and DMA could be recycled and reused, without obvious decrease of 3A5AF yield. Moreover, the formed 3A5AF could be purified by a facile procedure. Experiment results and density functional theory (DFT) calculations revealed that homogeneous Cl − was vital for 3A5AF formation. This study provides an example for the effective conversion of chitin biomass to value-added compounds.
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高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 4
·1-13
·
IF 5.3
Zhou Mengyan,Zhang Zhihui,Zheng Qi,Yu Mingdi,Si Mengxu,Wu Sirui,Zhang Yanan,Ding Shushu,Fu Ding-Yi
A novel ratiometric fluorescent nanohybrid probe was constructed for sensitive and selective determination of folic acid (FA) based on blue D-penicillamine-based carbon dots (CDs) and red glutathione S-transferase-Au nanoclusters (GST-AuNCs). Upon the excitation of 380 nm, the obtained CDs-NCs possessed two distinct emission peaks at 465 and 665 nm. The fluorescence intensity at 465 nm was incrementally enhanced with the addition of FA attributed to the hydrogen bonds formation, while the fluorescence intensity at 665 nm was quenched caused by the electronic interaction and the inner filter effects. The fluorescence intensity ratio ( I 465 /I 665 ) exhibited good linear correlation with FA concentrations in the range 10∼90 μM, and the limit of detection (LOD) was 0.63 μM. Notably, the corresponding fluorescent color changed from red to blue, which could be distinguished by naked eyes. On account of the excellent biocompatibility, the CDs-NCs were further successfully used for bioimaging and intracellular FA detection. Furthermore, the real sample analyses confirmed that the proposed nanoprobe could be expanded as a versatile platform for FA detection in practical applications. Graphical abstract
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Molecular Catalysis
·
2024
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IF 3.9
XiaoNing Gao,YanXiao Zhang,Wei Fu,Hong Zhou,Lu Feng
Conventional hydrolysis of total saponins for the extraction of diosgenin, an essential ingredient in the production of steroid hormones, has caused significant ecological pollution, making efficient and clean extraction methods necessary. Here, UiO-66-NH 2 with defect sites containing Zr-coordinated hydroxide and water groups was synthesized using concentrated HCl as a crystal modulator, which was further treated with trimethylsilyl trifluoromethanesulfonate (Me 3 SiOTf) to afford UiO-66-NH 2 OTf using the oxygen affinity of the Me 3 Si group. The strong Lewis acid UiO-66-NH 2 OTf was used to extract diosgenin from the total saponin, and the extraction temperature, extraction time and amount of addition were optimized using single-factor experiments and the response surface method. In addition, high-resolution mass spectrometry has been used to analyze the resulting compounds, thus suggesting an alcoholysis pathway and an alcoholysis mechanism for the total saponins.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Xiaoying Gao,Jiarui Zhu,Jiahui Zhao,Lei Zhao,Ying Sun,Jiazhen Lin,Mengli Hu,Yunjia Liu,Shenghong Yang,Jian Liu
A cascade colorimetric detection of salivary D-amino acids (DAAs) holds promise for the preliminary screening diagnosis of gastric cancer. Pursuing metal-organic complexes with high peroxidase-mimic (POD-mimic) activity is crucial to enhance diagnosis efficiency. In this work, we developed a straightforward strategy in a “one-pot” process to modulate the POD-mimic activity of CuX-trithiocyanuric acid (CuX-TTCA) complexes. By adjusting the molar ratios of CuX (Cl, Br, and I) and TTCA during synthesis to modify the coordination configuration of Cu(I) in CuX-TTCA, we easily tuned their POD-mimic activities. Among them, CuCl-TTCA-2 with a feeding molar ratio of 2:1 for CuCl:TTCA exhibited remarkable POD-mimic activity attributed to its exposed catalytic active sites and efficient mass transfer ability during catalysis. Long-lived 1 O 2 was identified as the primary reactive oxygen intermediate. A highly sensitive and selective cascade colorimetric detection platform was developed for two typical DAAs associated with gastric cancer, namely D-proline and D-alanine, achieving limit of detection values of 1.1 and 0.8 μM, respectively. As a proof-of-concept application, our cascade detection platform demonstrated excellent specificity in distinguishing saliva samples between gastric cancer patients and healthy individuals. The outstanding selectivity and reliable outcomes from DAAs assay make our detection platform highly promising for preliminary screening diagnosis of gastric cancer and patient self-detection applications. Our straightforward strategy for tuning POD-mimic activity provides an in-depth understanding on structure-activity relationship in nanozymes, offering valuable opportunities to advance enzyme-mimic optimization for other potential applications.
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Energy Storage Materials
·
2023
·
IF 20.4
Zhewen Ma,Zhongyuan Huang,Zhao Li,Guojie Chen,Yinghui Li,Wen Zhu,Wenguang Zhao,Haocheng Ji,Hui Fang,Wen Wen,Wen Yin,Jianxin Zou,Yinguo Xiao
In this study, ultrafine Mg(In) solid-solution particles were successfully prepared through an arc plasma method and their deuterium uptake/release performances were systematically estimated with respect to the identically processed pure Mg particles. Thermodynamic destabilization and kinetic enhancement of Mg-In-D system were achieved simultaneously with lower deuterium ab/desorption enthalpies and decreased desorption temperatures. For the first time, an in-situ synchrotron X-ray diffraction (ISXRD) coupled with neutron powder diffraction (NPD) technique and density functional theory (DFT) calculations clearly substantiated that an indium diffusion-dominated phase transition is the driving force for the evolution from mixture of MgD 2 and Mg-In intermetallic to Mg-based solid solution during the deuterium release (DR). The ISXRD results clarified the real-time phase transition from MgIn to Mg 3 In at 515 K and the interreaction of MgD 2 and Mg 3 In at 640 K, by which the matrix could be destabilized to trigger an indium-diffusion governed DR reaction in deuteride. Moreover, with the aid of NPD experiment, the microstructure evolution of Mg-In-D system demonstrated the continuous diffusion of indium into Mg/MgD 2 upon desorption. The solubilized indium in MgD 2 effectively reduced the D -migration energy barrier and stabilized the lattice structure to restrain the constriction of Mg-D bonds, through which the noticeable improvement on both thermodynamic and kinetic performances of the Mg-In system was achieved. The combination of synchrotron X-ray and neutron diffraction offers a high-efficiency strategy to fathom the hydrogen discharging mechanism from the viewpoint of real-time phase transitions and crystalline structure evolutions.
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高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2025
·
IF 4.7
Mingyu Wang,Haofei Sun,Xinyi Bai,Bo Su,Kai Li,Shengbo Zhang,Hailin Cui,Cunlin Zhang,Zhaoxin Geng
Hypertension directly or indirectly accounts for over 10% of maternal deaths. L-methyldopa is a safe antihypertensive for pregnant women, yet its low aqueous solubility substantially reduces bioavailability. This study enhances L-methyldopa's solubility in simulated gastric and intestinal fluids through cocrystallization with D-mannitol, thereby improving bioavailability. The cocrystal was synthesized via slow solvent evaporation. Then, multiple characterization techniques including powder X-ray diffraction (PXRD), Raman spectroscopy (Raman), scanning electron microscopy (SEM), and differential scanning calorimetry-thermogravimetric analysis (DSC-TGA) were employed to confirm the formation of cocrystals and verify the crystal structure and purity. Subsequently, a terahertz time-domain spectroscopy (THz-TDS) system was used to measure the spectral properties of D-mannitol, L-methyldopa, physical mixture, and cocrystal, and it was found that terahertz absorption spectroscopy can effectively characterize the formation of cocrystals. Furthermore, we also performed DFT calculations to simulate the crystal structures of D-mannitol, L-methyldopa, and the corresponding cocrystal. By comparing the experimental and simulated terahertz absorption spectra, it was found that they showed good agreement. High-performance liquid chromatography (HPLC) measurements showed that, compared with pure L-methyldopa, the solubility of L-methyldopa in the cocrystal in simulated intestinal fluid and gastric fluid increased by 53% and 67% respectively. DSC-TGA analysis confirmed significantly enhanced thermal stability of the cocrystal. This work provides valuable methodological insights for pharmaceutical cocrystal development.
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高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Yusheng Yuan,Xin Zhao,Shaopu Liu,Yuanfang Li,Ying Shi,Jingjing Yan,Xiaoli Hu
A specific and sensitive fluorometric method based on the Carbon dots (CDs) probe which show unique optical properties is proposed for the determination of D-Penicillamine (D-PA) in human serum samples with satisfactory results. The fluorescence of CDs decreased apparently in the presence of mercury ion (Hg 2+ ), Similarly, the addition of D-Penicillamine (D-PA) resulted in efficient fluorescence recovery of the CDs due to the strong affinity between Hg 2+ and D-PA. Herein, this phenomenon has been exploited to design an “AND” logic gate and specific fluorescence switch sensor for sensitively sensing D-PA for the first time. Furthermore, the CDs possesses bright blue fluorescence with the maximum excitation and emission wavelength at 358 nm and 442 nm, respectively. Thus, after the experimental conditions are optimized, the linear range for detection PA is 2.0–24.0 μ mol L −1 , with correlation coefficient and detection limit is R 2 = 0.998, 0.6 μ mol L −1 , respectively. Moreover, the proposed method is not only expected to be come a potential tool for the fast response of D-PA but also possesses the potential for practical application.
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危险品化学品经营许可证（带存储）