Analytical Methods
·
2016
·Vol. 8
·期 5
·1127-1134
·
IF 1.915
Ning An,Lijuan Wang,Jingjing Zhao,Lili Lv,Ning Wang,Huaizhong Guo
An interesting study on chiral nonaqueous capillary electrophoresis (NACE) was developed in this paper. Two new chiral selectors, a lactobionic acid–boric acid complex and a D-(+)-xylose–boric acid complex were respectively in situ synthesized in nonaqueous background electrolytes (BGEs) containing methanol and triethylamine. They were found to be applicable for the enantioseparation of fourteen amino alcohols including eight β-blockers and six β-agonists by NACE. In order to achieve good enantioseparation, the effects of chiral selector concentration, BGE composition, capillary temperature, and applied voltage were systematically investigated. Under the optimized conditions, most of the tested amino alcohols achieved good chiral resolution. The effects of the molecular structures of chiral selectors and analytes on enantioseparation were discussed in terms of molecular interactions. The method was proved to be suitable for routine analysis of propranolol enantiomers, since it provided satisfactory results during linearity, precision, and accuracy (recovery) studies using the lactobionic acid–boric acid complex as the chiral selector. Good linearity was obtained in the range of 1.0–100.0 μg mL−1 for each propranolol enantiomer and the recoveries for them ranged from 96.4% to 105.9% with the relative standard deviation (RSD) less than 6.4%. This chiral NACE method was applied for the determination of propranolol enantiomers from tablets and the average contents were 101.3% for (R)-propranolol and 98.5% for (S)-propranolol (n = 5).
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Carbon Neutrality
·
2025
·Vol. 4
·期 1
·1-9
·
IF 12.5
Yang Delong,Dai Jinhang,Du Ziting,Cao Qingya,Chen Gang,Qi Ting,Wang Weilu,Li Fukun
Since 3-acetylamino-5-acetylfuran (3A5AF) has been regarded as a promising nitrogen-containing synthon, the conversion of renewable chitin and its monomer N-acetyl-D-glucosamine (NAG) to 3A5AF is of significance. In this paper, we compared the catalytic activity of several Cl-containing compounds and found that LiCl exhibited superior performance. The effects of solvent, catalyst dosage, reaction temperature, and reaction time on the synthesis of 3A5AF were investigated. Under the optimized conditions, about 60% yields of 3A5AF were obtained in N-methyl-2-pyrrolidone (NMP) or dimethylacetamide (DMA) medium. The spent LiCl and DMA could be recycled and reused, without obvious decrease of 3A5AF yield. Moreover, the formed 3A5AF could be purified by a facile procedure. Experiment results and density functional theory (DFT) calculations revealed that homogeneous Cl − was vital for 3A5AF formation. This study provides an example for the effective conversion of chitin biomass to value-added compounds.
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JOURNAL OF MOLECULAR LIQUIDS
·
2025
·
IF 5.2
Hongyu Guo,Yanling Huang,Liangbin Lin,Shibing Chen,Fafu Yang
A high luminescence asymmetry factor (g lum ) and an excellent luminescence quantum yield in aggregated state are crucial factors for evaluating the application potential of circularly polarized luminescence (CPL) materials, yet achieving both simultaneously remains challenging. In this work, two AIE-active fluorogens based on two conjugated cyano-distyrylbenzenes attaching one or two cholesterol moieties with amide groups as spacers ( BiCDB-1Ch and BiCDB-2Ch ) were designed and synthesized. Both BiCDB-1Ch and BiCDB-2Ch showed the excellent CPL properties in aggregated states based on their hydrogen-bonding columnar liquid crystalline self-assembly. The absolute fluorescence quantum yield of BiCDB-1Ch with stronger D-A structure was 0.43 and the g lum value was as high as 0.30, which was the highest one among the reported columnar CPL liquid crystals. The detailed liquid crystalline self-assembly behaviour and the mechanism of efficient chirality transfer and amplification were elucidated, providing a strategic approach for developing the novel liquid crystalline materials with superior CPL performance.
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Journal of Materials Chemistry B
·
2021
·Vol. 9
·期 43
·9023-9030
·
IF 6.331
Lulu Kong,Di Fan,Lin Zhou,Shaohua Wei
Acute kidney injury (AKI) is a severe clinical disease with extremely high morbidity and mortality. It is challenging to find a simple method for early detection of AKI and monitoring the treatment results. Renal tubular damage and inflammation are early events in AKI. Renal tubular damage is conducive to the accumulation of small-sized nanoparticles in the kidney, and inflammation is related to the excessive production of H2O2. Recent studies proved that chiral molecule modification of nanomaterials is a powerful strategy to regulate their biodistribution. Thus, L-serine and D-serine modified poly(amidoamine) (PAMAM) dendrimers were synthesized and used as fluorescent probe (NPSH) carriers to obtain L-SPH and D-SPH, respectively. D-SPH has a strong accumulation capability in the kidney of AKI mice. Then, the H2O2 fluorescent probe can detect the excessively produced H2O2 to generate fluorescence to diagnose AKI. Subsequently, the anti-inflammatory drug manganese pentacarbonyl bromide (CORM) was loaded in D-SPH to obtain D-SPHC with AKI theragnostic functions. Simultaneously, the D-SPHC fluorescence signal intensity change during the treatment can be used to monitor the recovery process. This study is the first report of chiral materials used in the diagnosis and treatment of AKI.
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高影响因子
NUTRITIONAL NEUROSCIENCE
·
2025
·
IF 3.6
Jiahao Liu,Jiawei Li,Yuanyuan Liu,Ruici Liu,Liya Fang,Zhimei Jiang,Lei Liu,Luchuan Wang,Chao Gong,Deming Kong,Ruting Fu,Pei Zeng,Jin Guo,Shaobo Zhou
Objective Epilepsy treatments often lead to vitamin D (VitD) deficiency. Although vitamin D₃ (VitD3) has been shown to reduce epileptic symptoms by 43%, its preventive effects remain unclear. This study investigated the potential of VitD pretreatment in two common acute epilepsy mouse models and explored its effects on seizure severity, latency, and molecular mechanisms involving calcium-sensing receptor (CaSR), phosphatase and tensin homolog (PTEN), and autophagy.Methods Mice were randomly divided into nine groups (n = 15). VitD or vehicle was administered 40 min before pentylenetetrazole (PTZ) or kainic acid (KA) given intraperitoneal injection (i.p). Seizure behavior and electroencephalograms (EEGs) were recorded for 60 min. After 24 h, hippocampal tissues were analyzed histologically and assessed for expression of autophagy-related proteins, CaSR, and PTEN.Results Both PTZ and KA induced acute seizures (Racine Grade IV+), with corresponding high-amplitude EEG spikes, neuronal damage, and mossy fiber sprouting. CaSR and autophagy markers were upregulated, while PTEN was downregulated, especially in the KA group. VitD pretreatment reduced seizure frequency, prolonged latency, alleviated hippocampal damage, downregulated CaSR and autophagy markers, and upregulated PTEN. These effects were milder than those of valproate. Combined VitD and Oroxin B treatment further improved outcomes.Conclusions (1) PTZ-induced seizures increased CaSR and decreased PTEN, triggering autophagy and worsening symptoms. (2) KA-induced epilepsy caused more severe damage with stronger autophagy activation. (3) VitD pretreatment mitigated seizures by modulating CaSR, PTEN, and autophagy, showing greater efficacy in the PTZ model.
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ACS Catalysis
·
2025
·
IF 13.1
Fang Huang,Aihao Xu,Xiangyu Chen,Huanhuan Sun,Siyu He,Dong Wei,Boran Wang,Anxiang Guan,Xucai Yin,Jing Xu,Huibing He
The direct electrocatalytic conversion of carbon dioxide to methane using water as a medium is a reliable way to store intermittent renewable energy and solve environmental problems. However, in such multielectron/proton transfer reactions, the role of water is often overlooked. Specifically, the sluggish kinetics of water dissociation limit the effective proton supply during methane formation. Here, we propose a copper-doped CeO2 catalyst with frustrated Lewis pairs (FLPs), which can effectively reduce carbon dioxide to methane. Combined experimental analysis and theoretical calculations reveal that the synergistic interaction between Cu and FLPs forms an f-p-d gradient orbital coupling system, which significantly promotes water dissociation to generate active protons and optimizes the adsorption behavior of the *H and *COOH intermediates. Even at the large current density of −273 mA cm–2, the Faraday efficiency of Cu/CeO2-FLPs for methane was as high as 78.0%, with a conversion frequency of 15784.1 h–1 in the flow cell. This work provides a strategy for the rational design of efficient multipoint catalytic systems.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Yun Liu,Zhina Qiao,Hengwei Zhang,Xian Zhang,Yanan Li,Zhiming Rao
D-tagatose, a functional rare sugar, has physiological effects such as lowering blood sugar and anti-caries. However, research on the industrial production of D-tagatose has primarily focused on Escherichia coli , limiting application in the food field. In this study, a cell factory for efficient synthesis of D-tagatose was constructed using the GRAS strain Corynebacterium glutamicum . Firstly, the key enzyme L-arabinose isomerase from Lactobacillus fermentum was screened. Secondly, by rational design, the optimal mutant D390V/H339P significantly enhanced enzyme activity by 40.65 % and improved thermostability. Subsequently, a dual-enzyme coupled whole-cell system was established by regulating the ratio of β-galactosidase and L-arabinose isomerase by RBS optimization. After optimizing the catalytic system, 101.42 g/L D-tagatose was produced from 400 g/L lactose, and the conversion rate reached 25.12 % in 5 L fermenter, which is the highest yield using lactose as substrate and GRAS strain as chassis cells. This research laid a foundation for the industrial application of food-grade D-tagatose.
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高影响因子
PHYTOMEDICINE
·
2025
·
IF 8.3
Shaowei Zheng,Ya Gao,Houkang Cao,Kai Guo,Wenxiu Wang,Guiying Lu,Shasha Wang,Mingli Zhong,Bo Li,Kefeng Zhang,Riming Wei,Jianzhao Wu
Background Acute liver failure (ALF) is associated with ferroptosis and currently lacks specific drugs and therapies. Previous studies have shown the hepatoprotective activity of Sophoricoside (SOP) in diverse models of hepatic injury. However, the role of SOP in ALF caused by lipopolysaccharide (LPS)/D-galactosamine (D-GalN) is still unknown. Purpose To investigate the therapeutic effects of SOP on ALF and its underlying mechanisms of action. Methods In vivo , an ALF mouse model was established using LPS and D-GalN. RNA sequencing was used to identify differentially expressed genes, which were subsequently confirmed by Western blot and qRT-PCR. In vitro , erastin was used to induce ferroptosis in HepG2 cells. Subsequently, the cells were treated with SOP, the nuclear factor erythroid 2-related factor 2 (Nrf2) inhibitor ML385, and the glutathione peroxidase 4 (GPX4) inhibitor RSL3. Additionally, siRNA was utilized for Nrf2 knockdown. Measurements of biochemical markers, reactive oxygen species (ROS) levels, alterations in mitochondrial membrane potential, qRT-PCR, and Western blot analysis were used to confirm the effectiveness and mechanism of SOP. Finally, the interaction between SOP and Nrf2 was verified through molecular docking, surface plasmon resonance (SPR), and cellular thermal shift assay (CETSA). Results In vivo , experimental analysis revealed that SOP ameliorated liver injury, hepatocyte Fe 2+ accumulation, and oxidative stress. RNA sequencing analysis indicated that SOP reversed the abnormal expression of genes related to Nrf2 and the ferroptosis pathway. In vitro , SOP ameliorated erastin-induced oxidative stress, ROS accumulation, and mitochondrial membrane potential loss. After the application of pharmacological inhibitors and siRNA knockdown, the SOP’s inhibitory effect on ferroptosis was antagonized. Conclusion SOP alleviated ALF by activating the Nrf2/GPX4 signaling pathway, thereby mitigating oxidative stress and ferroptosis.
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高影响因子
Molecular Medicine Reports
·
2017
·Vol. 16
·期 2
·1298-1306
·
IF 1.692
Yuyan Liang,Hai Niu,Limei Ma,Dan Du,Li Wen,Qing Xia,Wen Huang
Coreopsis tinctoria (snow chrysanthemum) has been reported to exert antihyperlipidemic effects. The present study aimed to identify the active compounds of Coreopsis tinctoria and to investigate the molecular mechanisms underlying its effects on lipid dysregulation by measuring lipid levels, reactive oxygen species, lipid peroxidation and fatty acid synthesis. The present results demonstrated that snow chrysanthemum aqueous extracts significantly reduced serum lipid levels and oxidative stress in vivo. The main compounds that were isolated were identified as flavanomarein (compound 1) and eriodictyol 7‑O‑β‑D glucopyranoside (compound 2). Compounds 1 and 2 demonstrated potent antioxidative properties, including free radical scavenging activity, inhibition of lipid peroxidation, as well as lipid‑lowering effects in human HepG2 hepatocellular carcinoma cells treated with free fatty acids (FFAs). Compound 2 was revealed to suppress the elevation of triglyceride levels and inhibit lipid peroxidation following FFA treatment. In addition, it was demonstrated to significantly reduce intracellular levels of reactive oxygen species and improve the mitochondrial membrane potential and adenosine triphosphate levels, thus protecting mitochondrial function in FFA‑treated HepG2 cells. Furthermore, compound 2 markedly suppressed the protein expression levels of disulfide‑isomerase A3 precursor and fatty acid synthase, thus suppressing FFA‑induced lipogenesis in HepG2 cells. In conclusion, the present study identified compound 2 as one of the main active compounds in Coreopsis tinctoria responsible for its lipid‑lowering effects. Compound 2 was revealed to possess antihyperlipidemic properties, exerted via reducing oxidative stress, protecting mitochondrial function and suppressing lipogenesis.
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ACS Catalysis
·
2025
·
IF 13.1
Wei An,Zeyang Liu,Tongjun Shen,Yizhang Du,Cun Huang,Boyuan Duan,Chunxia Wang,Guoyong Huang,Yiming Yan,Shengming Xu
To overcome the low current-density efficiency and long-term stability issues of industrial overall water splitting at ampere-level current density, a kind of superwetting Mo-doped nanoarray anchored on nickel foam (NF) (Mo-CoP/NC) with three-dimensional (3D) nanoflower structure is constructed. The incorporation of Mo species induces thermoneutral hydrogen adsorption free energy and creates favorable empty d-orbitals, which enhances the d–p hybridization of Co–O bonds, thereby accelerating the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) kinetics in alkaline media. The optimized Mo-CoP/NC exhibits exceptional electrocatalytic performance, requiring low overpotentials of 198 mV for HER and 384 mV for OER at a current density of 1000 mA cm–2 and achieving maintains robust operation in overall water splitting at 3000 mA cm–2. When employed as a bifunctional catalyst in an alkaline electrolyzer, a cell voltage of 1.87 V can be realized at 1000 mA cm–2 , outperforming commercial benchmarks. Density functional theory (DFT) calculations reveal that Mo doping optimizes hydrogen adsorption energy and reduces energy barriers for water dissociation. The superwetting surface ensures rapid gas bubble detachment and efficient active site regeneration, while N-doped carbon encapsulation endows long-term stability with negligible activity decay over 100 h of continuous operation.
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SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Yusheng Yuan,Xin Zhao,Shaopu Liu,Yuanfang Li,Ying Shi,Jingjing Yan,Xiaoli Hu
A specific and sensitive fluorometric method based on the Carbon dots (CDs) probe which show unique optical properties is proposed for the determination of D-Penicillamine (D-PA) in human serum samples with satisfactory results. The fluorescence of CDs decreased apparently in the presence of mercury ion (Hg 2+ ), Similarly, the addition of D-Penicillamine (D-PA) resulted in efficient fluorescence recovery of the CDs due to the strong affinity between Hg 2+ and D-PA. Herein, this phenomenon has been exploited to design an “AND” logic gate and specific fluorescence switch sensor for sensitively sensing D-PA for the first time. Furthermore, the CDs possesses bright blue fluorescence with the maximum excitation and emission wavelength at 358 nm and 442 nm, respectively. Thus, after the experimental conditions are optimized, the linear range for detection PA is 2.0–24.0 μ mol L −1 , with correlation coefficient and detection limit is R 2 = 0.998, 0.6 μ mol L −1 , respectively. Moreover, the proposed method is not only expected to be come a potential tool for the fast response of D-PA but also possesses the potential for practical application.
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Journal of Water Reuse and Desalination
·
2018
·Vol. 9
·期 1
·18-30
·
IF 0.688
Wen Yue,Zhang Xingran,Chen Mei,Wu Zhichao,Wang Zhiwei
In this study, we systematically evaluated the antibiofouling behavior of quaternary ammonium compound (QAC) blended polyvinylidene fluoride (PVDF) membrane using quartz crystal microbalance with dissipation monitoring (QCM-D) combined with flow cytometry (FCM) and confocal laser scanning microscopy (CLSM) measurements. QCM-D tests showed that the introduction of QAC reduced bacterial attachment due to the biocidal functions of QAC. FCM indicated that cell integrity of the bacteria in the suspension flowing along QAC-modified membrane surfaces during the QCM-D test was severely affected. CLSM confirmed the significantly lower attachment of bacteria and higher dead/live cell ratio onto the surface of modified membranes after the washing step in QCM-D tests. Both FCM and CLSM results validated the antibacterial behavior of QAC-modified membranes by a contact-killing mechanism, which is in agreement with that of QCM-D tests. In addition, the bacterial cells accumulated on modified membrane surface exhibited higher reversibility compared to the control membrane, indicating ease of membrane cleaning. The results highlight that the combined use of QCM-D, FCM, and CLSM can comprehensively characterize the antibiofouling behavior of membranes.
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