JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2017
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IF 3.154
Dongliang Zhang,Yanfei Cao,Chengye Ma,Shanfeng Chen,Hongjun Li
There is a paradox when incorporating enzyme into an edible chitosan film that chitosan is dissolved in acid solution and enzyme activity is maintained under mild conditions. A method for maintaining the pH of the chitosan solution at 4–6 to prepare a chitosan film containing β-cyclodextrin, resveratrol−β-cyclodextrin inclusion (RCI), was developed, using glucamylase and acetic acid. A considerable amount of resveratrol was released by the glucamylase-incorporated film within 15 days, and the maximum amount released was 46% of the total resveratrol content. The highest resveratrol release ratio (released resveratrol/total resveratrol) was obtained in the film with 6 mL of RCI. Scratches and spores were generated on the surface of the glucamylase-added film immersed in water (GAFW) for 7 days because of β-cyclodextrin hydrolysis during film drying and water immersion. RCI and β-cyclodextrin were extruded from the film surface and formed teardrops, which were erased by water on the GAFW surface but appeared on the glucamylase-added film without water immersion (GAF). The bubbles generated by the reaction of acetic acid and residual sodium bicarbonate were observed in both glucamylase-free films immersed in water (GFFW) for 7 days and without water immersion (GFF). The FT-IR spectra illustrated that the covalent bond was not generated during water immersion and β-cyclodextrin hydrolysis. The crystal structure of chitosan was destroyed by water immersion and β-cyclodextrin hydrolysis, resulting in the lowest chitosan crystallization peak at 22°. The increasing of water holding capacity determined by EDX presented the following order: GAF, GFFW, GFF, and GAFW.
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CARBOHYDRATE POLYMERS
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2017
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IF 4.811
Zhen Zhang,Fujun Jin,Zicong Wu,Ju Jin,Feng Li,Yiliang Wang,Zhiping Wang,Shunqing Tang,Chaoxi Wu,Yifei Wang
Chitosan nanofibers (CSNFs) have potential applications in biomaterials, oil recovery and food packaging, but their instability in moist environment has limited their full utilization. Here we report that CSNFs can be O-acylated in a post-electrospinning treatment by using pyridine as catalyst and short-chain (C2, C3, C4, C5 and C6) and long-chain (C8 and C12) fatty acid anhydrates as acylation agents. The effects of O-acylation to CSNFs were analyzed in detail. FT-IR, 1 H NMR and elemental analysis indicated that the hydroxyl groups of chitosan in CSNFs were acylated in 2 h. XRD spectra indicated that the O-acylation modification altered the crystal structure of the native fibers and the acyl substituents packed in a laterally aligned and layered structure. SEM examinations showed that the acylation modification could effectively control the fibrous structure of CSNFs and improve their stability in moist environment. The O-acylated CSNFs generally have an average diameter about 100 nm except for laurelated CSNFs (∼200 nm). Water contact angle measurement indicated that the wetting properties of O-acylated CSNFs were affected by the length of acyl side chains. This fiber acylation strategy can tune the material properties of CSNFs and expand their potential applications.
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CARBOHYDRATE POLYMERS
·
2017
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IF 4.811
QingBo Xu,LiJing Xie,Helena Diao,Fang Li,YanYan Zhang,FeiYa Fu,XiangDong Liu
Carboxymethyl chitosan (CMCTS) and silver nanoparticles (Ag NPs) were successfully linked onto a cotton fabric surface through a simple mist modification process. The CMCTS binder was covalently linked to the cotton fabric via esterification and the Ag NPs were tightly adhered to the fiber surface by coordination bonds with the amine groups of CMCTS. As a result, the coating of Ag NPs on the cotton fabric showed excellent antibacterial properties and laundering durability. After 50 consecutive laundering cycles, the bacterial reduction rates (BR) against both S. aureus and E. coli remained over 95%. It has potential applications in a wide variety of fields such as sportswear, socks, and medical textile.
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ACS Biomaterials Science & Engineering
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2017
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IF 3.234
Hong-Yin Wang,Jie Sun,Liu-Yuan Xia,Yan-Hong Li,Zhan Chen,Fu-Gen Wu
Immunofluorescence staining is a crucial tool for studying the structure and behavior of intracellular proteins and organelles. During the staining process, the permeabilization treatment is usually required to enhance the penetration of a fluorescent antibody into the cells. However, since most of the membrane imaging dyes as well as the membrane lipids will detach from the cell surface after permeabilization, membrane labeling using these dyes is not compatible with immunofluorescence staining. Herein, by linking cholesterol-polyethylene glycol (PEG-Chol) and fluorescein isothiocyanate (FITC) with the amine-rich glycol chitosan (GC), we prepared a multifunctional polymeric construct, GC-PEG Chol-FITC, and realized permeabilization-tolerant plasma membrane imaging. Owing to the presence of abundant amine groups in the labeling reagent and the membrane proteins/lipids, the addition of paraformaldehyde in the fixation step induces the amine-cross-linking between the labeling reagents and the membrane proteins/lipids, thus preventing the detachment of fluorophores from the cell surface after permeabilization. Besides, the large molecular weight effect of the imaging reagent may also account for its antipermeabilization property. Furthermore, by combining immunofluorescence staining with the plasma membrane labeling by GC-PEG Chol-FITC, we simultaneously imaged the plasma membrane and cytoskeletons, and clearly observed metaphase cells and binucleated cells. The concept of using amine-rich polymeric dyes for plasma membrane imaging will inspire the development of more permeabilization-resistant membrane labeling dyes with better performance, which can realize simultaneous membrane and intracellular protein imaging and facilitate the future studies of membrane–intracellular protein interactions.
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FOOD CHEMISTRY
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2017
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IF 4.529
Jie Long,Bao Zhang,Xingfei Li,Xiaobei Zhan,Xueming Xu,Zhengjun Xie,Zhengyu Jin
In this study, pullulanase was firstly immobilized by covalent bonding onto chitosan/Fe 3 O 4 nanoparticles or encapsulation in sol-gel after bonding onto chitosan/Fe 3 O 4 nanoparticles, and then the immobilized pullulanase was used for the effective production of resistant starch (RS). The highest RS content (35.1%) was obtained under the optimized condition of pH 4.4, enzyme concentration of 10 ASPU/g and hydrolysis time of 12 h when debranched by free pullulsanase, indicating that RS content was significantly (p < 0.05) increased when compared to native starch (4.3%) and autoclaved starch (12.5%). Under these conditions, the immobilized pullulanase (10 ASPU/g dry starch) yielded higher RS content compared to free enzyme (10 ASPU/g dry starch), especially, the pullulanse immobilized by sol-gel encapsulation yielded the highest RS content (43.4%). Moreover, compared to starches hydrolyzed by free pullulanase, starches hydrolyzed by immobilized pullulanase showed a different saccharide profile of starch hydrolysate, including a stronger peak C (MW = 5.0 × 10 3 ), as well as exhibited an additional absorption peak around 140 °C. Reusability results demonstrated that pullulanase immobilized by sol-gel encapsulation had the advantages of producing higher RS content as well as better operational stability compared to pullulanase immobilized by cross-linking. The resulting enhanced RS content generated by the process described in this work could be used as an adjunct in food processing industries.
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CARBOHYDRATE POLYMERS
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2016
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IF 4.219
Zhong Lu,Jingting Gao,Qingfeng He,Jie Wu,Donghui Liang,Hao Yang,Rong Chen
Nano Ag/ZnO hybrid material has been considered to be a promising nanocomposites for biomedical application because it has enhanced antibacterial activity and low cytotoxicity. Here a sponge-like nano Ag/ZnO-loaded chitosan composite dressing was first synthesized via preparing a chitosan sponge by lyophilization process, followed by the incorporation of Ag/ZnO nanocomposites into chitosan sponge. The porosity, swelling, blood clotting and in vitro antibacterial activity against drug-sensitive and drug-resistant pathogenic bacteria were evaluated. The results demonstrate that the prepared composite dressing shows high porosity and swelling as well as enhanced blood clotting and antibacterial activity. Cytocompatibility test evaluated in vitro illustrates the very low toxic nature of the composite dressing. Furthermore, the in vivo evaluation in mice reveals that the chitosan-Ag/ZnO composite dressing enhances the wound healing and promotes re-epithelialization and collagen deposition. These results strongly support the possibility of using this novel chitosan-AgZnO composite dressing for wound care application.
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Journal of Materials Chemistry B
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2017
·Vol. 5
·期 19
·3521-3530
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IF 4.543
Yixian Zhang,Yiran Xu,Xiangyi Xi,Surakshya Shrestha,Pengfei Jiang,Wenjing Zhang,Changyou Gao
The extensive development and application of engineered nanoparticles (NPs) in various fields worldwide have been subjected to increasing concern due to their potential hazards to human health and the environment. Therefore, a simple, economical, and effective method for suppressing the toxicity of metal-based nanomaterials is needed. In this study, glutaraldehyde-crosslinked chitosan nanoparticles (CS NPs) were prepared and further modified with lysine (Ly-CS), glutamic acid (Glu-CS), or sodium borohydride reduction (R-CS), and used to suppress cytotoxicity induced by copper oxide NPs (CuO NPs) through chelation with intracellularly released copper ions. All three kinds of CS NPs had similar sizes of ∼100 nm in a dry state and ∼200 nm in cell culture medium, as determined by scanning electron microscopy, transmission electron microscopy, and dynamic light scattering. The chelating efficiency of different CS NPs followed the order Ly-CS > Glu-CS > R-CS. The CS NPs showed minimal or no toxicity to three different cell lines (HepG2, A549, and RAW264.7 cells) at 100 μg mL−1 with similar cell internalization and exocytosis processes. Comparatively, RAW264.7 cells exhibited higher endocytosis and exocytosis rates, as revealed by flow cytometry and confocal laser scanning microscopy. CS NPs were found as agglomerates inside A549 cells and RAW264.7 cells, with the amount of agglomerates inside RAW264.7 cells decreasing significantly with prolonged incubation. All three CS NPs, especially Ly-CS and Glu-CS NPs, efficiently suppressed the cytotoxicity induced by CuO NPs, and reduced the intracellular level of reactive oxygen species.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2016
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IF 3.138
Chao Chen,Yong Yu,Xiaoli Wang,Ping Shi,Yibing Wang,Ping Wang
Podophyllotoxin (PPT) offers a broad-spectrum of anticancer activities, but little has been reported for its controlled release. This work shows that by manipulating molecular interactions between PPT and Chitosan, efficient nanoscale capsulation of PPT can be realized. The drug encapsulation efficiency is as high as 52%, with a final particle drug loading in the order of 10% (wt/wt). It further demonstrates that changes in pH can also significantly affect the rate of drug release from the Chitosan nanoparticles. Upon contact with cancer cells, chitosan nanoparticles enable efficient internalization and drug release. In vitro evaluations with HepG-2 and MCF-7 cells indicate that the chitosan nanoparticle carriers can improve drug efficacy in comparison to free PPT, most likely by regulating the intrinsic apoptotic signaling pathway to induce apoptosis. Overall, PPT chitosan nanoparticles promise a safe and efficient drug delivery system for PPT.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Dongxue Fu,Guifang Wang,Li Zhao,Yuwen Hong,Baoming Yang,Huiling Cheng
Green ion-imprinting technique was applied to construct a Cr(III) ion-imprinted absorbent material from an amino functionalized chitosan derivative. First, nitrogenous organic compounds (GACS) as functional monomer is synthesized by a Schiff base reaction followed by a grafting reaction. In the end, imprinting was completed by cross-linking with epichlorohydrin (ECH) in aqueous media and eluting the coordinated ions with hydrochloric acid to obtain chromium-imprinted polymers (Cr(III)-IIP) with pores. The relevant parameters of the imprinting system were explored, and the superior polymer (Cr(III)-IIP 9 ) has an excellent adsorption capacity 385.89 mg/g, wide pH range adsorption, good adsorption rates and equilibrium times (≤120 min) for Cr(III) ion. Additionally, the kinetic study is best defined in terms of pseudo-second order, while the Freundlich isotherm model fits the data well. Competitive adsorption of the quaternary system of Cr(III)/ Cd(II)/ Ni(II)/ Cu(II) were also investigated, it demonstrated a greater affinity for the Cr(III) ion than other ions. Hence, the imprinting materials prepared by amino functionalized chitosan enhances the adsorption of Cr(III) ions in the simulation of actual water sample. The adsorption mechanism accords with the hard and soft acid and base theory (HSAB), which is expected to be is utilized for the separation of Cr(III) ions in the environment.
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FOOD HYDROCOLLOIDS
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2025
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IF 12.4
Baoyi Zhang,Haifeng Mai,Xinqi Liang,You Liu,Weijuan Huang
This study presents a general approach for fabricating polysaccharide-based self-healing emulsion gels with superior physical stability. The emulsion gels were synthesized by crosslinking carboxymethyl chitosan (CMC)-stabilized emulsions with oxidized sodium alginate (OSA). The self-healing properties of the gels were attributed to the formation of reversible imine bonds between the primary amino groups (-NH 2 ) of CMC and the aldehyde groups (-CHO) of OSA. Gelation occurred rapidly (20–60 s) upon mixing the CMC-stabilized emulsion with the OSA solution. Microstructural analysis revealed that the dispersed oil droplets were tightly encapsulated within an interconnected gel network. The compressive strength and rheological properties of the CMC-OSA emulsion gels were influenced by the mass ratio of CMC to OSA and the preparation parameters of OSA. Furthermore, the CMC-OSA emulsion gels demonstrated rapid self-healing at room temperature without external stimuli, along with excellent injectability. Owing to the 3D gel-like network structure and reversible interactions at the oil/water interface and within the continuous phase, the emulsion gels exhibited exceptional physical stability, including storage stability, freeze-thaw stability, pH and ionic stability, thermal stability, and centrifugal stability. This study provides a promising strategy for developing injectable self-healing emulsion gels with tunable mechanical properties and enhanced physical stability for multifunctional applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
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IF 7.7
Xiaoyao Wang,Haizhi Huang,Siyao Zhong,Xuping Shentu,Zihong Ye,Xiaoping Yu
Fenpropathrin residues in grain are potentially harmful to humans. Therefore, a fluorimetric lateral flow immunoassay using a zirconium-based organic skeleton (UiO-66) as a signal marker was developed for detecting fenpropathrin. Herein, carboxymethyl chitosan (CMCS) was used to modify UiO-66 and improve its water solubility to facilitate stable binding with sodium fluorescein (NaFL). This resulted in formation of a new fluorescent probe that is more suitable for lateral flow immunoassay (LFIA). The materials were characterized via electron microscopy , Fourier-transform infrared spectroscopy, and powder X-ray diffraction. CMCS and NaFL were successfully bound to UiO-66. Under optimized conditions, the constructed NaFL/UiO-66@CMCS-LFIA exhibited a good linear relationship within the range of 0.98–62.5 μg/L, with a detection limit of 3.91 μg/L. This probe was fourfold more sensitive than traditional colloidal gold nanoparticle-based LFIA. Finally, NaFL/UiO-66@CMCS-LFIA was successfully applied to detect fenpropathrin in wheat and maize samples. The detection limit was 1.56 μg/kg and recoveries ranged from 96.58 % to 118.56 %. This study provides a sensitive, stable, and convenient method for the rapid detection of pesticide residues .
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CARBOHYDRATE POLYMERS
·
2024
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IF 10.7
Yilong Wang,Xingyu Zhou,Junhui Jiang,Tianhao Zhao,Junbo Dang,Ruibo Hu,Chen Shen,Qiaochu Fan,Dahui Sun,Mei Zhang
Critical-sized bone defects (CSBDs) necessitate interventions like bone grafts or tissue engineering scaffolds to surpass the body's limited spontaneous healing capacity and ensure effective bone regeneration. A multi-level microstructured composite hydrogel 3D scaffold was fabricated for enhanced bone defect repair, integrating a 3D-printed macroporous polylactic acid (PLA) scaffold with polydopamine treatment and filled with a sodium alginate/nano hydroxyapatite/carboxymethyl chitosan (SA/nHA/CMCS) micrometer-scale porous composite hydrogel. The incorporation of nano hydroxyapatite (nHA) nanoparticles enhanced hydrogel crosslinking and osteogenic activity. A systematic evaluation of CMCS concentration demonstrated its pivotal role in enhancing hydrogel cross-linking and mineralization, regulating degradation rate adapted to the osteogenic cycle, endowing the scaffold with a bioactive micrometer-scale porous structure. In vitro studies confirmed the osteogenic effectiveness of the composite hydrogel 3D scaffold, particularly those with CMCS, which boosted bone mesenchymal stem cells (BMSCs) adhesion, proliferation, and differentiation. The rabbit tibial bone defect model further confirmed that, compared to the DAPLA (dopamine modified PLA) scaffold, the bone trabecular number of the DSHC (DAPLA-SA/nHA/CMCS) scaffold increases 2.06-fold. In conclusion, this study expanded the application of hydrogel scaffolds in bone tissue engineering and provided an effective strategy for the development of hydrogel implant materials.
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