Bone Reports
·
2024
·
IF 2.1
Qiang Chen,Shaoqin Tu,Dongxiao Tang,Jiaming Wei,Nan Wei,Hong Ai,Bu Yang,Zheng Chen
Objective To explore the effect of amyloid-β peptide (Aβ) on mandibular condyle to develop a new treatment for postmenopausal women with Temporomandibular joint osteoarthritis. Methods A murine bone loss model was established by ovariectomy. Microstructure parameters of the condyle were measured by microcomputed tomography before and after intraperitoneal injection with Aβ. Flow cytometry, Alizarin red staining, RT-qPCR assays, FITC/PI staining, Oil Red O staining and western blotting were used to evaluate the effect of Aβ on the osteogenic differentiation of mouse bone marrow stromal stem cells (mBMSCs). Results In vivo, condylar microstructure parameters increased. Serum osteoprotegerin and procollagen type 1 N propeptide increased in a dose-dependent manner after the injection of Aβ, which were opposite the changes observed in c-terminal telopeptides of type I collagen, tumor necrosis factor-α and the high serum level of leptin. In vitro, Aβ promoted calcium nodule formation in the cells. The expression of ALP, Runx2, osteorix and osteocalcin increased significantly. The expression of mRNAs related to the Wnt signaling pathway was significantly upregulated, which could be blocked by DKK1. Conclusion Aβ can reverse bone loss in the mandibular condyle in ovariectomized mice through promoting the osteogenic differentiation of mBMSCs via the Wnt pathway.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POLYMER RESEARCH
·
2025
·Vol. 32
·期 3
·1-10
·
IF 2.8
Feng Yanqing,Guo Penghui,Ding Wei,Jin Yushun,Liu Ruofan,Wu Yibo
The use of renewable bio-based monomers to prepare polyisobutylene-based polymers can not only reduce the dependence on petroleum resources but also promote the green transformation of the cationic polymerization industry. Herein, 2-chloro-2,4,4-trimethylpentane (TMPCl) was synthesized as the main initiator and then cooperated with co-initiator TiCl 4 to construct a cationic initiation system, which was successfully used in preparing poly(isobutylene- co - β -myrcene). The effects of co-initiator concentration, monomer concentration and monomer feed ratio on the copolymerization of isobutylene (IB) and β -myrcene were investigated. The results showed that the molecular weight (M n ) first increased and then decreased with elevating the concentration of TiCl 4 . When the monomer concentration was 15 wt% and the monomer feed ratio (mole ratio between IB and β -myrcene) was 98:2, an IB- co - β -myrcene random copolymer with a molecular weight of 7.3 × 10 3 g/mol and a lower T g (-67.97 ℃) was obtained. According to the 1 H NMR analysis of the polymer structure and terminal groups, it was suggested that IB and β -myrcene mainly formed the copolymer with 1,4 structural unit under this initiation system, thus the cationic polymerization mechanism of IB and β -myrcene was further proposed. This work can provide basic data and theoretical guidance for the manufacture of greener polyisobutylene-based polymers in the future.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 3
·1-12
·
IF 2.8
Huang Kai,Que Tao,Guo Xiang,Wang Rui,Ding Xiang,Zhou Hongqing
The advancement of high-density integration and packaging technologies necessitates the development of packaging materials that possess enhanced thermal conductivity and substantial mechanical strength. In this paper, we present a novel type of CaO–B 2 O 3 –SiO 2 /β-Si 3 N 4 composites synthesized through the solid-state reaction. The effects of β-Si 3 N 4 ceramic content on the sinterability, phase structure, microstructure, and properties of the composites were studied. As an excellent structural and functional material, the rod-like β-Si 3 N 4 ceramic not only enhanced the thermal conductivity (λ) and flexural strength of composite materials but also effectively reduced the thermal expansion coefficient (CTE) of the composite and maintained good high-frequency dielectric properties. The composite sample containing 15wt.% β-Si 3 N 4 demonstrated exceptional performance after sintering at 850 ℃ for 30 min, achieving a bulk density of 2.80 g/cm 3 , a relative permittivity of 6.52 with a dielectric loss of 1.94 × 10 –3 (15 GHz), a λ of 3.5 W/(m·K), a CTE value of 6.40 ppm/ ℃, and a flexural strength of 180.1 MPa. Additionally, the EDS line mapping indicates that there was no diffusion of silver at the interface, thereby affirming the favorable compatibility between the composite and the silver electrode. This characteristic positions the CBS/β-Si 3 N 4 composite as a potentially promising candidate for applications in electronic packaging.
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Nan Yang,Minhui Huang,Chao Gao,Junxian Hu,Yantao Liu,Katsuyoshi Nishinari
Self-assembled protein fibers have attracted much attention in the fields of medicine and food because of their high aspect ratio, polymorphic structure and strong surface hydrophobicity . In this study, three different gelation types of polysaccharides/ β -lactoglobulin fiber (Fblg) composite gels, including ionic alginate-Fblg gels, synergistic xanthan-Fblg gels, and double network agar-Fblg gels, were first prepared. The interactions between the polysaccharides and the Fblgs, the microstructure and mechanical properties of the composite gels were investigated using the light scattering, scanning electron microscopy, rheology and texture analysis in order to reveal their formation mechanisms. Then the loading and release properties of the water-soluble drug 5-fluorouracil (5-FU) and the hydrophobic drug curcumin (Cur) through these composite gels were further studied with release mechanisms determined by fitting different release models. It was found that the mechanical properties of the composite gels were determined by the mesh density of the three-dimensional networks formed inside the gels. The network structure and mechanical strength of the alginate-Fblg gels became weaker with the increase of Fblg content at pH 4 due to their attractive interaction which hindered the binding of Ca 2+ to ALG, while the network and the strength of the alginate-Fblg gels didn't change much at pH 7 due to the repulsion between Alg and Fblg. The xanthan-Fblg gels formed lamellar structures with enhanced gel network and mechanical strength due to the hydrogen bonding and the electrostatic interaction with Fblg. The Agar-Fblg composite gel formed at 60 °C (above the gelation temperature of agar of 40 °C) had a denser double network structure and higher mechanical strength than that formed at 0 °C due to inhibition of diffusion of Ca 2+ as salt bridges for Fblg. The hydrophilic drugs were loaded in the meshes of the composite gels and their release was determined by the structure of the composite gel networks, whereas the hydrophobic drugs were loaded by attaching to the Fblgs in the composite gels and their release was determined by the loading ability and strength of the gels. The study not only provided a new idea for the preparation and application of polysaccharide-protein fiber composite hydrogels, but also provided insights for improving the efficiency of drug carriers.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Tongguo Huo,Kai Cao,Jianxin Zheng,Dan Zhu,Yuan Lin,Yu Dai,Jian Wu
The mechanism of β-SiC preparation via chemical vapor deposition (CVD) of the SiCl4–CH4–H2–N2 system remains unclear. Consequently, the change of molar Gibbs free energy of the CVD β-SiC chemical reaction in the SiCl4–CH4–H2–N2 system has been studied by the Helsinki Software Corporation (HSC) Chemistry code for the first time. The role of nitrogen in the reaction was confirmed. Seven potential reaction pathways of CVD β-SiC were presented, and the thermodynamic equilibrium components of each reaction were calculated systematically. The most viable reaction pathway and corresponding optimal temperature range for CVD β-SiC were determined. In addition, a kinetic study of CVD β-SiC was conducted. The microscopic morphology and crystal structure of β-SiC coatings prepared on the graphite surface at different temperatures were charactered by scanning electron microscopy (SEM), X-ray diffraction (XRD), Raman, etc. Ultimately, through SEM, XRD, and Raman observation, uniform and dense β-SiC coatings with fine grains and high crystallinity were successfully obtained. Furthermore, large β-SiC-coated graphite trays with diameters of 230 and 465 mm were prepared by CVD using the SiCl4–CH4–H2–N2 system, and the average thickness of β-SiC was about 100.6 μm. This study provides a theoretical basis and technical recommendations for the fabrication of SiC-coated graphite trays used in metal–organic chemical vapor deposition (MOCVD) equipment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Keyu Lin,Zhongyu Shi,Zheyi Zhang,Yunfan Wei,Sibao Wan,Haiyan Gao,Zhen Qin
β-1,3-1,4-Glucanases have attracted significant interest because of their potential applications in various food industries. Glycoside hydrolase (GH) family 148 β-1,3-1,4-glucanases exhibit excellent thermal stability and a unique catalytic mechanism, making them particularly promising for certain food processing applications. This study investigated the module architecture enzymatic properties, catalytic mechanisms, and potential applications of a multi-domain β-1,3-1,4-glucanase (EngU) from GH family 148. The overall structure of EngU comprises three independently folding domains, namely the GH148 catalytic domain, CBM4, and the C-terminal domain. The insertion of CBM4 causes the catalytic domain of EngU to be non-contiguous in sequence. The optimum reaction conditions for EngU have been found to be pH 6.0 and 90 °C, demonstrating relatively high thermostability. EngU is an atypical β-1,3-1,4-glucanase, predominantly cleaves the β-1,3 glycosidic bonds in β-1,3-1,4-glucan. Malt saccharification experiments revealed that adding EngU (80 U/g malt) can decreased the viscosity of mash by 6.85 % and the filtration time by 28.83 %. Furthermore, EngU was found to effectively hydrolyze oat bran, producing β-glucooligosaccharides, with the main hydrolysis products being trisaccharide and disaccharide. These results demonstrate the unique module architecture and the application potential of GH family 148 β-1,3-1,4-glucanases, providing valuable insights and resources regarding this category of glycoside hydrolases.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Yu Yang,Xianbing Song,Manman Wang,Xiaolian Li,Ziqiang Wang,Peng Wang
β-Alanine is an important non-essential amino acid and used in the manufacture of a wide range of drugs, in particular it plays a key role in the biosynthesis of carnosine and pantothenic acid. L-aspartate-α-decarboxylase (PanD) is the key enzyme in the enzymatic preparation of β-alanine. In this study, BsPanD* was modified by protein structure prediction and directed evolution, and a mutant BsPanD* PQ4 with good catalytic properties was obtained, which showed a 40 % increase in activity and remained stable in the pH 6.0–9.0 range. The strain E.coli pRSF Duet-1 - smaiA - ecaspA (pMA) was constructed for efficient co-expression of maleate cis - trans isomerase and L-aspartase, with an apparent activity of 253.4 U/g. The multi-enzyme cascade catalytic process for the preparation of β-alanine with maleate as the substrate was developed. The results showed that when the concentration of ammonium maleate was 1 mol/L, the substrate conversion rate was greater than 99.9 %, and the β-alanine concentration was 87.5 g/L with a yield of 98.3 %. This study not only solved the problem of low activity and poor stability of PanD, but also realized the preparation of β-alanine from maleate, a cheap chemical raw material, and laid the foundation for its industrial application.
✓ 已验证
产品关联
DOI
高影响因子
Food Science & Nutrition
·
2024
·
IF 3.5
Yong-Hui Wang,Sheng-Hua He,Ji-Hong Huang,Wei-Yun Guo,Xue-Li Gao,Guang-Hui Li
Moderate non-covalent interaction of protein and polyphenols can improve the emulsifying property of protein itself. The corn protein hydrolysate (CPH) and tannic acid (TA) complex was successfully used to construct nanoemulsion for algal oil delivery. There has been no study on the feasibility of this nanoemulsion delivery system for other food functional components, for example, β-carotene (β-CE). CPH/TA complex-based nanoemulsion system for β-CE delivery was studied, focusing on the effect of β-CE content on the physicochemical stability of the nanoemulsions. The nanoemulsion delivery systems (dia. 150 nm) with low viscosity and good liquidity were easily fabricated by two-step emulsification. The nanoemulsions with high β-CE content (>71.5 μg/mL) significantly increased ( p < .05) the emulsion droplet size. However, there was no significant ( p > .05) effect of β-CE content on polydispersity index (PDI) and zeta potential of the nanoemulsions. The storage (30 days) experiment results demonstrated that the droplet size of the nanoemulsions with varying β-CE content increased slightly during storage. However, the PDI values showed a slightly decreasing trend. Zeta potentials of the nanoemulsions showed no noticeable change during storage. Moreover, after storage of 30 days, the retention ratios of β-CE were found to be up to 90%, which suggests an excellent protective effect for β-CE by the nanoemulsion systems. The CPH/TA complex stabilized nanoemulsions could aggregate in gastric condition, but the β-CE content did not have obvious effect on the digestive stability of the nanoemulsions. The CPH/TA complex could be employed as an emulsifier to construct a physicochemical stable nanoemulsion delivery system for lipophilic active components.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Nannan Cui,Zhikun Wang,Fengting Li,Huiling Su,Wentao Qu,Miantuo Li,Shuangqing Sun,Songqing Hu
Micro/nanocontainers, as smart carriers of corrosion inhibitors, capable of intelligently detecting corroded areas and dispensing inhibitors precisely for targeted corrosion control. Here, pH-responsive chitosan- grafted -β-cyclodextrin (CS- g -β-CD) microspheres (CgCM) loaded with various corrosion inhibitors are prepared via an emulsification cross-linking method. By FT-IR and 1 H NMR characterizations, the successful loading of corrosion inhibitor BTA into β-CD is achieved, with the obtained BTA@β-CD being further grafted on the CS chain by degree 28.8 %. The prepared CgCM possess a size range from 15 to 20 μm, and a surface zeta potential range from positive in acidic solution (33.7 mV at pH = 3) to negative in alkaline solution (−20.4 mV at pH = 10). In addition to loading BTA into β-CD, three compound agents (AIM, AMIM-Cl, and 3-SAMA) are selected to be loaded into the crosslinking gaps of the CgCM, with the loading ratios of BTA, AIM, AMIM-Cl, and 3-SAMA determined to be 31.59 %, 13.85 %, 16.93 %, and 19.30 %, respectively. When subjected to acidic stimulation, the CgCM undergo swelling and dissociation, leading to the responsive release of the four loaded inhibitors into the solution, for which the accumulative release ratios (48 h, pH = 3) are determined to be 93 %, 86 %, 84 %, and 81 %, respectively. Weight loss and electrochemical evaluations firmly establish that these CgCM, laden with inhibitors, are adept at forming dense protective barriers on steel surfaces, achieving a corrosion inhibition efficiency exceeding 90 %. Corrosion morphology, surface elemental distribution, and binding status reveal a compact compounding protective layer formation on the steel/solution interface, by a physicochemical synergistic adsorption pattern. Density functional theory (DFT) calculations and molecular dynamics (MD) simulations reinforce the findings, revealing that all three CgCM systems exhibit targeted, stimulus-responsive release capabilities, coupled with robust adsorption strengths and remarkable corrosion resistance characteristics.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2025
·
IF 6.9
Lu Chen,Zhengkai Wu,Xueying Cheng,Shuyu Chen,Jiaxin Zhao,Hang Li,Yingna Guo,Qingkun Shang
In this paper, Bi 4 O 5 I 2 /α-Bi 2 O 3 composite photocatalysts were successfully prepared by loading Bi 4 O 5 I 2 nanosheets on the surface of α-Bi 2 O 3 nanorods. The photocatalytic performance was investigated for the degradation of propranolol under visible light. Bi 4 O 5 I 2 /α-Bi 2 O 3 (30) could degrade 95 % of propranolol within 120 min, and the degradation rates of propranolol were 64.3 and 2.68 times that of α-Bi 2 O 3 and Bi 4 O 5 I 2 , respectively. The mineralization degree of propranolol reached 54.7 %, which was also much higher than that of Bi 4 O 5 I 2 and α-Bi 2 O 3 alone. Based on the results of high-resolution mass spectrometry (HRMS), the possible degradation pathways and products of propranolol were proposed, and the environmental toxicity of the degradation products was evaluated. The formation mechanism of Bi 4 O 5 I 2 /α-Bi 2 O 3 Z-type heterojunction was proposed by investigating the band structure and photoelectric properties of the composite photocatalyst. This work provides a successful example for the construction and application of new heterojunction photocatalysts.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ELECTRONIC MATERIALS
·
2025
·
IF 2.5
Wang Fangyu,Wen Yijie,Guo Xin,Zhang Xuanhao,Hu Shuai,Yuan Maohui,Han Kai
Upconversion nanomaterials have been extensively applied in many fields owing to their unique optical and electronic properties. In this work, β-NaYF 4 :Yb 3+ /Ho 3+ microcrystals were synthesized by an improved hydrothermal method. The morphology and the composition of the prepared microcrystals were experimentally investigated in detail by scanning electron microscopy (SEM) and X-ray diffraction (XRD). Under the excitation of a 980 nm continuous-wave (CW) laser, the green (541 nm) and red (644 nm) upconversion luminescence (UCL) of Ho 3+ through the energy transfer upconversion processes assisted by the Yb 3+ ions were experimentally studied at the single-particle level. With the gradual increase of excitation intensity in the highly doped Yb 3+ microcrystals, the red UCL was enhanced significantly more than the green UCL and was dominant, resulting in the UCL color finally tuning to pure red. Comparatively, for the lightly doped Yb 3+ microcrystals, the green UCL exhibited a relatively similar increase to the red UCL. The populations of the Yb 3+ and Ho 3+ ions and transitions of the UCL were systematically demonstrated through calculation of the energy levels. Notably, a new UCL at 580 nm was observed, which was ascribed to the transition of 5 F 1 , 5 G 6 → 5 I 7 .
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Jinlong Qin,Min Sun,Jiajing Cheng,Huici Jiang,Mingchen Lv,Jianxing Jing,Ran Chen,Zhen Fan,Jianzhong Du
Unavoidable damage to the basal layer of the endometrium has a huge negative impact on a woman’s reproductive health and menstrual cycle. However, it is difficult for medicine to penetrate a series of biological barriers toward the basal layer in the deeper area of the endometrium. To meet this challenge, we developed an ultrasound-responsive hydrogel incorporated with a transforming growth factor-beta (TGF-β) mimetic peptide to enhance pregnancy outcomes by restoring the function of a wounded endometrium due to its deep-tissue-penetration capability. In vitro studies revealed that the TGF-β-mimetic-peptide-loaded hydrogel could achieve 64.35% of cell migration under ultrasound stimulation even in phosphate-buffered saline of pH 6.0. Upon in situ sonication at the uterus, carboxymethyl chitosan can be released from degraded hydrogel to open tight junctions with reduced interstitial pressure by ultrasound to promote deep penetration. Rat studies revealed that the penetration capability of TGF-β-mimetic-peptide-loaded hydrogel with sonication was 1.6 times higher than that of the control group. Besides the rat uterine model, ex vivo human uterine tissue was also collected and imaged, demonstrating up to ∼700 μm of tissue penetration depth. In addition, compared to control groups, effective uterus recovery without intrauterine stenosis and endometrial cavity fluid was observed from rats with severe uterine injury treated by TGF-β-mimetic-peptide-loaded hydrogel. In addition, fertility restoration in the endometrial injury model was observed after treatment with such an ultrasound-responsive hydrogel incorporated with TGF-β mimetic peptides. Overall, this work provides an effective approach to treating endometrial injury for enhanced pregnancy outcomes.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）