TALANTA
·
2022
·
IF 6.556
Kai Zhang,Zhenqiang Fan,Yue Huang,Yuedi Ding,Minhao Xie,Minghe Wang
In this work, we designed an ECL ratiometric biosensor with a three-stranded Y-type DNA (Y-DNA) probe and induced a hybridization chain reaction (HCR) for the highly sensitive detection of SARS-CoV-2 nucleic acid. The important component of this system is the self-assembled Y-Shaped probe based on three nucleic acids. Y1, Y2, and Y3 can be linked by complementary base pairing to Hairpin1 (H1), Hairpin2 (H2), and Ru modified DNA (Ru1), respectively. H1 and H2 can trigger the HCR reaction when activated by the SARS-CoV-2 RdRp gene and the 5′ end of Ru1. The 5′ end of Ru1 is modified with the Ru complex, which can produce a strong electrochemiluminescence luminescence signal at 620 nm under an applied voltage. Through the amplification of Y-DNA-induced HCR reaction, Ru1 on the electrode surface gradually increased, the ECL signal at 460 nm was gradually quenched, and the signal at 620 nm was steadily generated. The SARS-CoV-2 RdRp gene can be quantified according to the degree of decrease of ECL signal at 460 nm and the increase of ECL signal at 620 nm. Combining the two signal amplification strategies, this ratiometric ECL biosensor can accurately and efficiently detect the target gene with a detection limit of 59 aM.
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JOURNAL OF CHEMICAL AND ENGINEERING DATA
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2022
·
IF 3.119
Guangyu Xu,Fanyuan Zhang,Zhenghui Li
In this study, the solubility of 5-chloropyrazin-2-amine in four aqueous solutions (methanol, ethanol, n-propanol, and ethylene glycol (EG)) plus water was determined by a gravimetric method at temperatures ranging from T = 278.15 to 323.15 K at 101.2 kPa pressure. Experimental results indicated that 5-chloropyrazin-2-amine solubility positively increased with increasing temperature at a given solvent composition and decreased with increasing mass fraction of water in each system. The order of solubility from high to low on the mole fraction scale was as follows: methanol (0.02007, 323.15 K) > ethanol (0.01308, 323.15 K) > n-propanol (3.827 × 10–3, 323.15 K) > water (1.569 × 10–3, 323.15 K). Similarly, the solubility of 5-chloropyrazin-2-amine was higher in (methanol + water) than in the other three cosolvent mixtures. The solid phase crystal of 5-chloropyrazin-2-amine was tested by X-ray powder diffraction (XPRD) and the results showed no polymorphic transformation, solvate formation, or crystal transition during entire experiments. The experimental solubility values of 5-chloropyrazin-2-amine were correlated by three thermodynamic models. The results show that the largest relative average deviation (RAD) and root mean square deviation (RMSD) data were no more than 5.38% and 2.34 × 10–4, respectively. The Jouyban–Acree model gave the best correlation result in this study.
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JOURNAL OF POLYMER RESEARCH
·
2022
·Vol. 29
·期 2
·1-9
·
IF 3.061
Song Tao,Wang Qingyin,Li Jianguo,Chen Xuejun,Liu Shaoying,Wang Gongying
A new copolyesters of poly(2-[4-(2-hydroxyethoxy)phenoxy]ethyl-co-1,4-cyclohexylenedimethylene terephthalate) (PHCT) with different compositions were synthesized from terephthalic acid, 1,4-cyclohexanedimethanol and hydroquinone bis(2-hydroxyethyl) ether (HQEE) via esterification-polycondensation reaction. The synthesized copolyesters were analyzed by 1 H-NMR, FT-IR, GPC, intrinsic viscosity, DSC, TGA and tensile testing, respectively. The 1 H-NMR and FT-IR analysis confirm the successful introduction of HQEE into poly(1,4-cyclohexylenedimethylene terephthalate (PCT) chains. The introduction of HQEE into PCT chains can effectively lower the melting point of PCT, making it easier to process. The glass transition temperature of PHCT is higher than 70 ℃ and it has the comparable thermal stabilities to PCT. In addition, introducing HQEE into PCT chains can effectively improve the molecular weights of PCT, thus imparting PHCT with better mechanical performance relative to PCT. The PHCT is a tough polymer, which owns the higher Young’s modulus (above 300 MPa), the higher tensile strength (above 39 MPa) and the higher elongation at break (above 226%).
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RSC Advances
·
2022
·Vol. 12
·期 20
·12932-12937
·
IF 4.036
Shaoying Sun,Xing Zhang,Yanqing Li,Xianzhao Shao,Jianwei Ji,Junhai Liu,Wei Wang,Zhizhou Li,Xiaohui Ji
New solid acid catalysts were prepared by bisphenol A, paraformaldehyde and chlorosulfonic acid, and applied to hydroxylalkylation/alkylation (HAA) of 2-methylfuran (2-MF) and cyclohexanone. After optimizing the reaction conditions, the conversion of 2-MF reached 99% and the yield of 5,5′-(cyclohexane-1,1-diyl)bis(2-methylfuran) acquired 98%. The activity and catalytic efficiency were higher than those of Amberlyst 15 and Amberlyst 36 resins, which could be rationalized by high acid strength. At the same time, the catalysts were characterized by acid–base titration and FTIR. Hydrodeoxygenation (HDO) of HAA products of 2-methylfuran and cyclopentanone were processed on the Ni/SiO2 catalyst prepared by wet impregnation method to further convert into aviation kerosene, and the yield reached 93%.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2022
·
IF 9.136
Xiaohui Wang,Xue Wang,Yali Wang,Quanguo Zhai,Yucheng Jiang,Shuni Li
Octahedral molecular sieve – 5 (OMS-5), a typical 2 × 4 tunnel manganese oxide, is considered as an adsorbent for rubidium ion (Rb + ) because of its specific tunnel structure. Also, manganese oxide-based nanomaterials are also widely used in supercapacitor energy storage and capacitor deionization electrodes due to their high specific capacitance. In this work, a series of protonated OMS-5 (H-OMS-5, H-Co-OMS-5, and H-Ni-OMS-5) nanobelts are synthesized and used as electrode materials for electrochemical enrichment of Rb + in aqueous solution. The specific capacity values for H-OMS-5, H-Co-OMS-5, and H-Ni-OMS-5 at 1 A·g −1 are of 159, 168, and 215 F·g −1 , respectively. Thanks to the unique protonated 2 × 4 tunnel structure, well regulating surface area, and excellent specific capacitance, the maximum Rb + adsorption capacities of H-OMS-5, H-Co-OMS-5, and H-Ni-OMS-5 can reach to 390.3, 405.7, and 481.7 mg·g −1 , respectively. These results indicate that the protonated 2 × 4 tunnel manganese dioxide nanobelts are of great potentials as faradaic electrode materials for selective rubidium ion enrichment applications.
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Frontiers in Nutrition
·
2022
·
IF 6.59
Qianqian Yao,Linlin Fan,Nan Zheng,Christophe Blecker,Véronique Delcenserie,Huiying Li,Jiaqi Wang
Gut microbiota dysbiosis, together with goblet cells dysfunction has been observed in ulcerative colitis cases. This study aims to evaluate the potential of 2′-fucosyllactose (2′-FL) supplementation in inhibiting intestinal inflammation through regulating gut microbiota, protecting goblet cells, and stimulating mucin secretion. 2′-FL was orally administered to C57BL/6J mice daily (400 mg/kg bw) for 21 days and 5% dextran sulfate sodium (DSS) was used to induce the colitis in the last 7 days. Meanwhile, fecal microbiota transplantation (FMT) was conducted to test the roles of gut microbiota in the remission of colitis by 2′-FL. Gut microbiota alteration was analyzed through 16S ribosomal RNA (16S rRNA) sequencing. Periodic acid-Schiff (PAS), immunofluorescence staining, as well as mucin 2 (MUC2) and NOD-like receptor family pyrin domain containing 6 (NLRP6) messenger RNA (mRNA) expression in colon fragments was performed and detected. The results showed that the DSS + 2′-FL mice were found to have a slower rate of weight loss, lower disease activity index (DAI) scores, and longer colon lengths than the DSS group ( p < 0.05), so in the FMT recipient mice which received fecal microbiota from the DSS + 2′-FL group. In addition, the data revealed that 2′-FL relieved the disorder of DSS-induced gut microbiota, including decreasing the high abundance of mucin-utilizing bacteria in the DSS group, such as Bacteroides, Lachnospiraceae NK4A136, Lachnospiraceae , and Bacteroides vulgatus . PAS and immunofluorescence staining showed that 2′-FL treatment promoted the recovery of goblet cells and enhanced MUC2 and NLRP6 expression, which was also observed in the FM (DSS + 2′-FL) group. Moreover, NLRP6, which has been proved to be a negative regulator for Toll-like receptor 4/myeloid differential protein-8/nuclear factor-kappa B (TLR4/MyD88/NF-κB) pathway, was upregulated by 2′-FL in colon tissue. In conclusion, this study suggests that 2′-FL ameliorates colitis in a gut microbiota-dependent manner. The underlying protective mechanism associates with the recovery of goblet cells number and improves MUC2 secretion through TLR4-related pathway.
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INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Dan Zhao,Wen Liu,Ya-Nan Li,Rui-Juan Zhang,Li-Na Zhang
A modulated structure with symmetrical characteristic higher than three-dimensional is a fascinating crystallographic type, and it is randomly encountered by researchers. Herein, we prepared 0.1 mm level single crystals of Na3La(VO4)2 and determined its structure to be a (3 + 1)-dimensional modulated structure using the X-ray diffraction analysis method for single crystals. Its super space group was determined to be Pca21(0β0)000. On the other hand, we introduced Tb3+ into the Na3La(VO4)2 host lattice to fabricate phosphors Na3La1-x(VO4)2:xTb3+ and studied their photoluminescence properties. Interestingly, for the strong absorption of the Na3La(VO4)2 host lattice in the range of 200–400 nm, the traditional 330–385 nm light is unable to efficiently excite Tb3+ ions in the Na3La(VO4)2 host to generate luminescence of Tb3+. Instead, Na3La1–x(VO4)2:xTb3+ is suitable to be excited by 487 nm to generate emitting light at 543, 584, and 622 nm, due to Tb3+ characteristic 4f → 4f transitions of 5D4 → 7FJ (J = 5, 4, 3). Hence, the Tb3+-doped Na3La(VO4)2 phosphors have potential applications for white-light-emitting diodes.
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ELECTROPHORESIS
·
2022
·Vol. 43
·期 15
·1601-1610
·
IF 3.595
Ying Zhang,Ming Zhao,Cheng-Bo Wang,Yu Wang,Stanislas Nsanzamahoro,Ling-Ling Zhu,Wei-Feng Wang,Jun-Li Yang
Prolyl hydroxylase domain 2 (PHD2) is a key enzyme regulating the expression of hypoxia inducible factor (HIF). Its inhibitors can improve the expression of HIF and downstream genes, which can treat hypoxia-related diseases. Therefore, the establishment of a reliable PHD2 inhibitors screening method is of great significance for the drug development of hypoxia-related diseases. In this work, an accurate, rapid, and simple screening method for PHD2 inhibitors was introduced by capillary zone electrophoresis (CZE). In order to improve the detection sensitivity, the derivative reaction of α-ketoglutaric acid (α-OG) and 1,2-diaminobenzene (OPD) was used to enhance the UV absorption of α-OG (the substrate in the enzymatic reaction). The CZE method selected 20 mM Na 2 B 4 O 7 buffer (pH 9.0) as the separation buffer, +25 kV as the separation voltage, 25°C as the cartridge temperature, and 210 nm as the detection wavelength. Under this condition, the analysis of a single sample can be realized within 9 min. Compared with the existing reported methods, the present work can directly screen the PHD2 inhibitory activity of traditional Chinese medicine (TCM) extracts, which is of significance for the target-purification of bioactive individual compounds from TCMs. Under the optimal conditions, the PHD2 inhibitor screening platform was successfully established, and it was found that 70% methanol/water extracts of Astragali Radix and Codonopsis pilosula had good PHD2 inhibitory activity. Furthermore, the present work provides a novel approach for screening the PHD2 inhibitory activity of TCM extracts and the discovery of anti-hypoxia bioactive compounds.
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Analytical Cellular Pathology
·
2022
·
IF 4.133
Yingru Zhang,Yiyang Zhao,Jingwen Liu,Chunpu Li,Ying Feng,Shasha Jiang,Xiaoting Sun,Xueqing Hu,Yan Wang
Tanshinone IIA (TanIIA) is the main active ingredient in the fat-soluble components isolated from Salvia miltiorrhiza Bunge. Our previous studies have convincingly proved that TanIIA is an effective drug against human colorectal carcinoma cells. In order to further demonstrate the effect of TanIIA on CRC, we carried out exploratory research about it in vivo and in vitro. The results demonstrated that TanIIA were observably more effective than control group in preventing tumor growth, and it has increased the survival time. Cancer cells viability and proliferation were accompanied by concentration and time dependent decline reached with TanIIA. We found that TanIIA altered the morphology of cytoskeleton and it could obviously induce apoptosis of colorectal cancer cells and block the cells in the G0/G1 phase. TanIIA also increased phosphorylation of p38MAPK, upregulated ATF-2 expression and downregulated Transgelin-2 expression, which could be reversed by SB203580, a p38MAPK-specific inhibitor. Our results suggested that TanIIA could induce apoptosis of colorectal cancer and block the cells in G0/G1 phase involved in downregulating the expression of Transgelin-2 through p38MAPK signal pathway.
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CARBOHYDRATE POLYMERS
·
2022
·
IF 10.723
Lihua Geng,Quanbin Zhang,Qishan Suo,Jing Wang,Yingxia Wang,Cong Wang,Ning Wu
COVID-19 caused by SARS-CoV-2 has spread around the world at an unprecedented rate. A more homogeneous oligo-porphyran with mean molecular weight of 2.1 kD, named OP145, was separated from Pyropia yezoensis . NMR analysis showed OP145 was mainly composed of →3)-β- d -Gal-(1 → 4)-α- l -Gal (6S) repeating units with few replacement of 3,6-anhydride, and the molar ratio was 1:0.85:0.11. MALDI-TOF MS revealed OP145 contained mainly tetrasulfate-oligogalactan with Dp range from 4 to 10 and with no more than two 3,6-anhydro-α- l -Gal replacement. The inhibitory activity of OP145 against SARS-CoV-2 was investigated in vitro and in silico. OP145 could bind to Spike glycoprotein (S-protein) through SPR result, and pseudovirus tests confirmed that OP145 could inhibite the infection with an EC50 of 37.52 μg/mL. Molecular docking simulated the interaction between the main component of OP145 and S-protein. All the results indicated that OP145 had the potency to treat and prevent COVID-19.
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Catalysts
·
2022
·Vol. 12
·期 10
·1100
·
IF 4.501
Jingxin Tan,Guoqiang Wei,Zhen Wang,Hui Su,Lingtao Liu,Chunhu Li,Junjie Bian
Zn1−xCdxS catalysts with Zeolitic Imidazolate Framework-8 (ZIF-8) as the precursor were successfully prepared by ion exchange method, and the ability and electrochemical properties of a series of ZIF-8, ZnS and Zn1−xCdxS catalysts in photocatalytic degradation of 2-CP and TC were investigated. Doping of Cd ions was able to modulate the ZnS band gap width and improve the utilization of visible light by the photocatalyst. The nanocage catalysts with hollow structure of Zn1−xCdxS have better photocatalytic response. The removal of photocatalytic pollutants was up to 90% under optimal conditions. Using a Peroxymonosulfate (PMS)-assisted system to improve the degradation efficiency of 2-chlorophenol and tetracycline hydrochloride under visible light, we present a possible mechanism of Zn1−xCdxS as a photocatalyst for degradation in persistent pollutants and in PMS-assisted photocatalysis. Four active species, O2−, h+, -OH, and SO4•−, can be generated to degrade 2-chlorophenol and tetracycline hydrochloride under PMS-assisted activation. Zn1−xCdxS nanocage with high activity and stability provides a feasible approach to catalytically remove persistent pollutants from aqueous solutions under visible light conditions.Keywords:nanocage photocatalyst;Zn1−xCdxS;2-CP;TC;PMS;degradation
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2022
·Vol. 24
·期 37
·22710-22715
·
IF 3.945
Dongyuan Yang,Yanjun Min,Baihui Feng,Xueming Yang,Guorong Wu
The S1(1ππ*) state decay dynamics of 2-pyridone excited around the 000 band origin is investigated using femtosecond time-resolved photoelectron imaging technique. At a pump wavelength of 334.0 nm, the vibrational ground state and a few low energy vibrational states covered by the bandwidth of the pump laser pulses are excited. The lifetimes of the vibrational states show strong dependence on the vibrational energy and mode. A quantum beat between two lowest energy vibrational states is also observed. This study provides quantitative information about the vibrational-state dependent lifetime of the S1 state of 2-pyridone.
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