Food Chemistry-X
·
2022
·
IF 6.443
Meilin Jing,Qingqing Jiang,Yamin Zhu,Daming Fan,Mingfu Wang,Yueliang Zhao
The effect of acrolein on the formation of the 2-amino-1-methyl-6-phenylimidazo[4,5- b ]pyridine (PhIP) was investigated in a chemical model. Acrolein was found to increase PhIP formation at each tested addition level. 0–0.2 mmol of acrolein increased PhIP formation dose-dependently, while high levels of acrolein (>0.2 mmol) did not further increase PhIP formation. Mechanistic study showed that acrolein addition decreased the residue of phenylalanine and creatinine, but increased the content of some key intermediates. Further analysis indicated that acrolein can react with phenylalanine, creatinine, and PhIP to form adducts. These results suggested that acrolein was able to contribute to PhIP formation as a consequence of its comprehensive ability to facilitate Strecker degradation of phenylalanine and react with phenylalanine, creatinine, and PhIP. In addition, oxidation of the tilapia fish increased the PhIP formation in the roasted fish patties, further supporting the potential contribution role of lipid oxidation products to the formation of PhIP.
✓ 已验证
DOI
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Xiaoqiang Huang,Guohua Ru,Hengyun Tian,Pengbo Zhang,Hongchang Zhao,Shouren Zhang,Sijie Wang,An Jia,Wanli Zhao
Background Benign prostatic hyperplasia (BPH) significantly compromises patients' quality of life, has detrimental effects on both physical and mental well-being, and poses a substantial economic burden. Current pharmacological interventions primarily focus on symptom relief; nevertheless, their associated adverse effects are evident. Rape bee pollen and maca are both homologous food products, which are not only rich in nutrition, but also have many biological activities. There is no report on the combination of rape bee pollen and maca in benign prostatic hyperplasia therapy, and no report on the molecular mechanism. This study aims to systematically investigate the therapeutic efficacy and underlying molecular mechanisms of a chewable tablet formulation containing rape bee pollen and maca in the management of BPH. Methods First, the chewable tablets were prepared from rape bee pollen and maca, and the High Performance Liquid Chromatography (HPLC) fingerprint was established. During a 4-week period, rats were subjected to subcutaneous injections of testosterone propionate solution to establish a BPH model, while simultaneously receiving orally administered chewable tablets for BPH treatment via gavage. We assessed the body weight, prostate tissue index, serum levels of dihydrotestosterone (DHT), testosterone (T), estradiol (E2), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), interleukin-1beta (IL-1β), superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione (GSH) levels in rats. Prostate tissues were collected for histopathological analysis. The protein expression levels of IL-6, phosphorylated Janus kinase 2 ( p -JAK2), and phosphorylated signal transducer and activator of transcription 3 ( p -STAT3) were determined by Western blotting. Rat feces were collected aseptically for 16S rDNA gene sequencing to assess gut microbiota levels. Additionally, our research findings demonstrate that the combined application of rape pollen and maca exhibits superior therapeutic efficacy compared to the individual use of either rape pollen or maca alone. Results We prepared the rape bee pollen and maca chewable tablets, established the HPLC fingerprint map, and quantitatively analyzed four of these main components. We found that the rape bee pollen and maca chewable tablets significantly inhibited DHT, T, E2, E2/T ratio, IL-6, TNF-α, and IL-1β and reduced MDA levels and increased SOD, GSH levels. 16S rDNA gene sequencing revealed that the chewable tablet increased the abundance of the beneficial bacteria Faecalibacterium, Collinsella, Phascolarctobacterium, Kineothrix , and Lactobacillus , and decreased that of the pro-inflammatory bacterium Prevotella . Western blot indicated that the chewable tablet could significantly inhibit the IL-6, p -JAK2, and p -STAT3 protein expression levels. Conclusion We found that rape bee pollen and maca chewable tablets had a protective effect on rats with BPH, and also found that the combination of rape bee pollen and maca was better than separate application. The mechanism may be related to the gut microbiota and IL-6/JAK2/STAT3 signaling pathway.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2023
·
IF 4.1
Jiana Lin,Zenghui Xie,Yuling Hu,Gongke Li,Qisheng Zhong
Supramolecular macrocycle-based covalent organic frameworks (COFs) are promising adsorbents for adsorption of hazards due to their host-guest recognition property. However, most supramolecular macrocycles are conformationally flexible, making them challenging to introduce into COFs. In this work, a calix[6]arene-based COF (CX6-BD COF) was fabricated with a unique flower-like morphology and high crystallinity. Especially, the cavity of CX6 exhibited host-guest inclusion interaction for sulfonamides (SAs), which was verified by quantum chemistry calculation. The integration of the porosity of COFs with the recognition cavity of CX6 made CX6-BD COF display excellent enrichment performance for SAs, with good enrichment factors (EFs) between 77 and 96. The material was employed as an adsorbent for COF membrane filter extraction, coupled with ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) to simultaneously enrich and determine seven SAs in animal-derived food. The analytical method showed a wide linear range (0.01–100 µg/L and 0.05–100 µg/L) and low detection limits (3–10 ng/L). The established method was successfully applied to sensitively determine SAs in chicken, pork and beef samples, which achieved satisfactory recoveries (73.8–113%). These results demonstrated CX6-BD COF has good application potential in determination of trace and ultra-trace SAs in complex food matrices as an adsorbent.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Xiao-Lu Jie,Zi-Rui Luo,Jin Yu,Zhe-Ren Tong,Qiao-Qiao Li,Jia-Hui Wu,Yi Tao,Pei-Shi Feng,Ji-Ping Lan,Ping Wang
Ethnopharmacological relevance Pi-Pa-Run-Fei-Tang (PPRFT) is an empirical TCM prescription for treating asthma. However, the underlying mechanisms of PPRFT in asthma treatment have yet to be elucidated. Recent advances have revealed that some natural components could ameliorate asthma injury by affecting host metabolism. Untargeted metabolomics can be used to better understand the biological mechanisms underlying asthma development and identify early biomarkers that can help advance treatment. Aim of the study The aim of this study was to verification the efficacy of PPRFT in the treatment of asthma and to preliminarily explore its mechanism. Materials and methods A mouse asthma model was built by OVA induction. Inflammatory cell in BALF was counted. The level of IL-6, IL-1β, and TNF-α in BALF were measured. The levels of IgE in the serum and EPO , NO , SOD , GSH-Px, and MDA in the lung tissue were measured. Furthermore, pathological damage to the lung tissues was detected to evaluate the protective effects of PPRFT. The serum metabolomic profiles of PPRFT in asthmatic mice were determined by GC-MS. The regulatory effects on mechanism pathways of PPRFT in asthmatic mice were explored via immunohistochemical staining and western blotting analysis. Results PPRFT displayed lung-protective effects through decreasing oxidative stress, airway inflammation , and lung tissue damage in OVA-induced mice, which was demonstrated by decreasing inflammatory cell levels, IL-6, IL-1β, and TNF-α levels in BALF, and IgE levels in serum, decreasing EPO, NO, and MDA levels in lung tissue, elevating SOD and GSH-Px levels in lung tissue and lung histopathological changes. In addition, PPRFT could regulate the imbalance in Th17/Treg cell ratios, suppress RORγt, and increase the expression of IL-10 and Foxp3 in the lung. Moreover, PPRFT treatment led to decreased expression of IL-6, p-JAK2/Jak2, p-STAT3/STAT3, IL-17, NF-κB, p-AKT/AKT, and p-PI3K/PI3K. Serum metabolomics analysis revealed that 35 metabolites were significantly different among different groups. Pathway enrichment analysis indicated that 31 pathways were involved. Moreover, correlation analysis and metabolic pathway analysis identified three key metabolic pathways: galactose metabolism; tricarboxylic acid cycle; and glycine, serine , and threonine metabolism. Conclusion This research indicated that PPRFT treatment not only attenuates the clinical symptoms of asthma but is also involved in regulating serum metabolism. The anti-asthmatic activity of PPRFT may be associated with the regulatory effects of IL-6/JAK2/STAT3/IL-17 and PI3K/AKT/NF-κB mechanistic pathways.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Food Measurement and Characterization
·
2025
·
IF 3.3
Yang Tingting,Tao Guanhua,Xu Xinye,Yu Chunyu,Shi Jindou,Ma Qingwen
Phytosterol (PS) face challenges of poor stability and rapid release in the gastrointestinal tract. To overcome these limitations, this study developed whey protein-chitosan composite (WP-CS-PS) nanoparticles via reverse solvent precipitation, significantly enhancing PS transport efficiency and functional performance. Through systematic optimization using single-factor and orthogonal tests, the nanoparticles were fabricated under ideal parameters. The optimal conditions included a 1:1 WP/CS mass ratio, 0.8% (w/v) PS concentration, 60 °C hydration, and 15 min stirring, which yielded high encapsulation efficiency (77.43%), uniform particle size (203.41 nm, PDI 0.265), and excellent colloidal stability (zeta potential − 35.42 mV). Structural analyses (SEM, FT-IR) revealed a dense gel-network morphology with PS successfully embedded via hydrogen bonding and electrostatic interactions. The WP-CS matrix markedly improved PS stability against thermal, UV, and storage stresses, while in vitro digestion assays demonstrated sustained intestinal release (17.72% slower than free PS). These results not only validate WP-CS-PS as a robust carrier for lipophilic bioactives but also offer a scalable strategy to enhance the bioavailability of PS in functional foods, potentially addressing global health needs such as cholesterol management and dietary supplementation. By bridging material science and nutritional delivery, this work paves the way for sustainable nutraceutical innovations with broad industrial applicability. Graphical
✓ 已验证
产品关联
DOI
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-12
·
IF 15.7
Luo Jiayao,Guo Bangjun,Li Nana,Huang Qianjin,Wang Jingjing,Fu Yongzhu,Jia Linan,Zhang Xi,Hou Junbo,Zhu Jinhui,Zhuang Xiaodong
Coating Ni-rich layered oxide positive electrodes is essential to improve their electrochemical performance in sulfide-based all-solid-state Li batteries, but achieving cost-effective, high-performance positive electrodes remains challenging. In this work, we apply a thin (~3 nm) conductive binary Li borate glass coating (0.5Li 2 O·0.5B 2 O 3 ) onto single-crystal LiNi 0.8 Co 0.1 Mn 0.1 O 2 using a simple dry process and heating. This coated positive electrode delivers 209 mAh g −1 specific capacity at a specific current of 20 mA g −1 with 79.7% initial Coulombic efficiency, retains 87.8% capacity after 1000 cycles at a specific current of 200 mA g −1 , and achieves 14.6 mAh cm −2 areal capacity. Pouch cells with this positive electrode reach 383 Wh kg −1 specific energy, and sustain 300 cycles at a specific current of 66.67 mA g −1 . Various characterizations reveal that this coating can enhance Li-ion transport, stabilize the positive electrode lattice, and strengthen the interface between positive electrode and sulfide electrolyte. Here we show that conductive glass coatings enable high-voltage positive electrodes with high stability and specific energy in all-solid-state batteries.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT AND SOIL
·
2025
·
IF 4.1
Song Yang,Liu Yang,Li Haipu,Liu Qianwen,Lu Denglong,Yang Zhaoguang
Background and Aims Sulfur (S) decreases arsenic (As) accumulation in crops, yet its mechanism and applicability to other plants remain unclear. This study investigated the role of S in As behavior in lettuces ( Lactuca sativa L.) and evaluated S application in leafy plants, focusing on As complexation. Methods Plants were stressed with arsenate (AsV) and dimethylarsinic acid (DMA) under three different S supplementation (0, 1, and 3 mM) in hydroponic culture. As species, As-phytochelatins, ascorbate–glutathione (AsA-GSH) cycle enzymes and mineral elements in plants were analyzed. Results The effect of S was As-species-dependent. Specifically, under AsV stress, 3 mM S significantly decreased As content by 20.8% in leaves and As translocation factor (TF) by 17.4%, while root As (230–252 mg/kg, dry weight) and As-phytochelatins (1.02–1.27 mg/kg, fresh weight) remained unaffected, which is contrary to other reports. In terms of As species, leaf arsenite (AsIII) content and TF-AsIII were decreased, but AsV and root AsIII content were not significantly changed. Under DMA stress, 3 mM S significantly decreased As content by 17.7% in roots but did not alter leaf As content. The root DMA was significantly decreased, while monomethylarsonic acid (MMA) and leaf DMA content were unchanged. Additionally, exogenous S regulated root AsA-GSH cycle over leaves under both As stresses and increased leaf Fe and Zn accumulation. Conclusions Exogenous S inhibited AsV and DMA uptake, suppressed AsV-to-AsIII conversion and AsIII transfer to leaves, and facilitated DMA-to-MMA conversion, highlighting its potential applications in agriculture to restrict As in leaves on contaminated lands.
✓ 已验证
产品关联
DOI
高影响因子
Chinese Journal of Integrative Medicine
·
2025
·Vol. 31
·期 11
·982-990
·
IF 2.5
Chen Chang-feng,Gao Yin-huan,Fang Qin,Zhou Yong-feng,Liu Yong,Wu Jian,Chen Hao,Wang Lie-cheng,Chen Lei
Objective To elucidate the modulation mechanism of Suanzaoren Decoction (SZRD) on basolateral amygdala (BLA) neuronal activity to alleviate chronic restraint stress (CRS)-related behavioral deficits. Methods The male C57BL/6J mice were assigned to 4 groups using the complete randomization method, including control (CON, n =19), CRS ( n =19), SZRD ( n =21), and fluoxetine (Flu, n =22) groups. Mice were restrained for 6 h per day, over a 21-d period to establish CRS models. The CON group remained in their cages without food or water during the 6-h matching period. SZRD and Flu groups received intragastric administration of SZRD (4.68 g/kg) and Flu (20 mg/kg) daily, respectively, 30 min before restraint for 21 consecutive days. The therapeutic effects of SZRD were evaluated using behavioral tests including the tail suspension test, elevated plus maze test, and forced swimming test. The cellular Fletcher B. Judson murine osteosarcoma proto-oncogene (c-Fos) expression in the BLA was measured using immunofluorescence, while action potential (AP) firing and synaptic transmission in BLA pyramidal neurons were evaluated using whole-cell patch-clamp recordings. Results SZRD administration significantly increased time spent in the open arms and open-arm entries while reducing immobility time ( P <0.05 or P <0.01). It downregulated CRS-induced c-Fos expression and AP firing of pyramidal neurons in the BLA ( P <0.01). Additionally, SZRD selectively attenuated excitatory ( P <0.01), but not inhibitory, synaptic transmission onto BLA pyramidal neurons. Conclusion SZRD alleviated CRS-induced anxiety- and depression-like behaviors in mice by modulating the excitability and synaptic transmission of BLA pyramidal neurons.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
WATER AIR AND SOIL POLLUTION
·
2025
·Vol. 237
·期 1
·9
·
IF 3
Liu Yiqing,Hu Hong,Chen Chuanzeng,Xu Linghan
Generally, Fe(II) has an efficiently catalytic activity toward peroxymonosulfate (PMS), but the slow conversion of Fe(III) to Fe(II) and production of iron sludge impede the practical application of Fe(II)/PMS system. In this work, ascorbic acid (AA), a complexing agent and reductant, was introduced to Fe(III)/PMS process to facilitate the Fe(III) reduction. The decoloration of rhodamine B (RhB) was obviously enhanced in Fe(III)/PMS process with the addition of AA. Fe(III)-AA complex was firstly generated in Fe(III)/AA/PMS process via the complexation and then was reduced to Fe(II), which could catalyze PMS to yield oxidative species. Fe(IV), sulfate radical (SO 4 •− ) and hydroxyl radical ( • OH) were the working oxidants for RhB decoloration in this system, and SO 4 •− played a major role. Increasing Fe(III), AA or PMS dosage was beneficial to RhB decoloration, but their excessive concentration would hinder RhB decoloration. The existence of HCO 3 − , SO 4 2− or FA in this system showed an inhibition effect on RhB decoloration, while a facilitation effect was obtained when the high concentration of Cl − was present. This research offers a way to improve PMS activation by Fe(III) and a technology for eliminating the refractory organic pollutants from the contaminated water.
✓ 已验证
产品关联
DOI
高影响因子
Signal Transduction and Targeted Therapy
·
2026
·Vol. 11
·期 1
·80
·
IF 81.2
Mu Min,Li Hui,Chen Bo,Fan Rangrang,Zhong Kunhong,Wang Guoqing,Feng Chenqian,Chen Nianyong,Guo Gang,Tong Aiping,Zou Bingwen
The broader clinical application of Bispecific T-cell engagers (BiTEs) is hindered by their short half-life, on-target off-tumor toxicity, and limited therapeutic effect for solid tumors. Herein, we constructed a bimetallic-enriched triple-kill nanobomb manganese/Co 2+ -dopamine@BiTE/HPT (MnO 2 /Co-DA@BiTE/HPT) based on metal-polyphenol to improve the immunosuppressive tumor microenvironment by activating innate and adaptive immunity, thereby enhancing the treatment efficacy of BiTEs (PD-L1/CD3). A hyaluronic acid-modified PD-L1 aptamer (HPT) was introduced to improve the active targeting of the nanobombs and bind with PD-L1 overexpressing colorectal cancer. Bimetallic (Mn 2+ /Co 2+ ) activated the STING pathway; simultaneously, photothermal therapy (PTT) induces DNA fragmentation to cooperate with bimetallic to amplify the STING signal to “heat” the “cold” tumor microenvironment. The “hot” tumor with a large amount of T-cell infiltration facilitated BiTE recruitment of T cells to kill tumor cells. Furthermore, the efficient therapeutic potency of the triple-kill nanobombs (STING, BiTE, and PTT) was determined in subcutaneous colorectal cancer, distal, lung metastasis, and postoperative recurrence models, which indicated that MnO 2 /Co-DA@BiTE/HPT could improve the immune microenvironment, produce long-term immune memory, inhibit tumor growth, and prevent tumor recurrence and metastasis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2026
·
IF 18.1
Xiao Gang,Ma Xiaotao,Ma Bingyun,Chen Zhaochang,Qi Xingxiang,Shen Chao,Li Jiayi,Yang Yinan,Lin Zewan,Yao Shendong,Yue Jianing,Duan Shiyu,Yang Weifeng,Zhang Xuan,Cheng Tao,Ma Pibo,Shao Yuanlong,Zhang Jin
Aerogel fibers (AFs), while promising for personal thermal management, are constrained by an inherent trade-off between mechanical robustness and thermal insulating performance. To resolve this mechanical-thermal coupling issue, we propose an ion-mediated attenuated Coulombic assembly of recyclable heterocyclic aramid nanofibers (HANFs) to construct hierarchical AFs, enabling programmable manipulation of pore size and porosity. HANF-AF features a dual-scale porous architecture, comprising a nano-porous core and a macro-porous cellular shell, which encloses interconnected nanofiber spacing with sub-50 nm pores. The hierarchically porous architecture and strong crosslinking endow HANF-AF with a synergy of tensile strength up to 83.1 MPa and thermal conductivity down to 22.0 mW·m -1 ·K -1 . Their robustness enables knitting into an industrial-grade textile with superior insulating features over conventional thermal insulating fibers. By leveraging ion-mediated hierarchical assembly of recycled nano-building blocks, this work establishes a scalable and sustainable strategy for engineering multifunctional nanofibrous architectures, offering a blueprint for creating next-generation flexible, durable, and superinsulating materials for advanced thermal management.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL PAPERS
·
2026
·
IF 2.7
Xian Qiaoling,Liu Youqiang,Zhou Jian,Zhang Xuemin,Kong Yan
λ Fe–C micro-electrolysis was optimized to treat high-strength sulfonic acid wastewater.
λ Fe–C micro-electrolysis was optimized for high-strength sulfonic acid wastewater.
λ UV–Vis and FTIR identified methylsulfonic and alkylbenzenesulfonic acids.
λ The degradation of sulfonic acid wastewater followed a pseudo-second-order kinetic model (R 2 = 0.996).
λ Sulfonic acid wastewater degradation followed pseudo-second-order kinetics.
λ Economic assessment confirmed feasibility at a cost of 5.0 USD/m 3 .
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）