ChemNanoMat
·
2021
·Vol. 8
·期 2
·e202100461
·
IF 3.154
Zhi-Kai Cao,Jun Zhang,Yan-Chao Yu,Bing-Chang Wang,Xiao-Han Bai,Jun Yang,Yi-Tao Liang,Seeram Ramakrishna,Yun-Ze Long
Graphical Electrospun NaYF 4 :Yb 3+ ,Er 3+ @PLLA nanofibers introduce pH-sensitive functional groups and are load with DOX drug. After the DOX release, the photoluminescence of nanofiberat 550 nm becomes stronger under NIR 980 nm exciatation, which could be used to monitor the release of drug in real time. Stimulus-responsive nanofibers can adjust the speed of drug delivery, and real-time monitoring elucidates the relationship between drug release and therapeutic efficacy. Both of these advantages have great potential in clinical therapy. However, the complex preparation of current stimulus-responsive drug-loaded nanofibers leads to low drug-loading levels, limiting their widespread applications. In this study, we prepared pH-responsive drug-loaded nanofibers with fluorescence monitoring properties using the electrospinning method. A representative drug of DOX was loaded onto the nanofibers. The degradable flexible nanofibers modified by amino groups attained higher drug loading and controlled drug release. Precisely, the rate of drug release varies with different pH. Faster release speed occurs in the tumor microenvironment with faintly acidic conditions than in healthy tissues with neutral conditions. The pH-responsive mechanism was due to hydrazone bonds between the amino groups and DOX molecules, which FTIR confirmed. In addition, these pH-responsive nanofibers with intense upconversion fluorescence enable more sensitive monitoring, whereas weak luminescence cannot achieve.
✓ 已验证
产品关联
DOI
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2021
·
IF 5.537
Kaibo Yu,Lei Zhou,Jing Xu,Fenghua Jiang,Zhiwei Zhong,Liqiang Zou,Wei Liu
The postharvest preservation of Brassica vegetables has received much attention for improving their nutritional and commercial value. In this study, carboxymethyl cellulose (CMC)-based cross-linking coating embedded with liquid paraffin was prepared to preserve fresh pakchoi ( Brassica chinensis L.) and its effects on postharvest water loss, quality and reactive oxygen species (ROS) metabolism were evaluated. Results showed that ionic cross-linking and liquid paraffin reinforced the structure compactness and water barrier property of CMC coating by uniformly distributing liquid paraffin in cross-linking network. The novel CMC-based coating effectively reduced water loss and improved appearance and nutrition of fresh pakchoi stored at 25 °C and 60 % relative humidity for 48 h. Furthermore, the coating treatment enhanced ROS scavenging capacity and reduced lipid peroxidation, which was attributed to the improvement of antioxidant enzyme activities, ascorbate-glutathione cycle and phenylpropanoid metabolism. This work demonstrates that the novel CMC-based water barrier coating is promising to control the water loss of pakchoi and further maintain the whole quality by regulating ROS metabolism.
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Xizhuang Yue,Xueyan Xu,Chuansheng Liu,Shuang Zhao
The present work proposes a voltammetric sensor for the simultaneous determination of cefotaxime (CEF) and nimesulide (NIM) using poly(L-cysteine) and graphene composite modified glassy carbon electrode (GR/ p (L-Cys)/GCE). The electrochemical behaviors of CEF and NIM at the GR/ p (L-Cys)/GCE were characterized by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). Poly(L-cysteine) and graphene produce a synergetic catalytic effect on the electrochemical oxidation of CEF and NIM. Under optimized conditions, the well-defined and separated oxidation peaks of CEF and NIM were observed at 0.72 V and 0.97 V, respectively. The oxidation peak currents of CEF and NIM are linear to the concentrations over the range of 1.0 μM – 60.0 μM and 1.0 μM – 55.0 μM with the detection limits of 0.4 μM and 0.3 μM, respectively. The GR/ p (L-Cys)/GCE enabled the simultaneous determination of CEF and NIM in tap water and lake water samples, and the recoveries from 94% to 107% were obtained.
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Nanomedicine
·
2021
·
IF 6.4
Chuan Fu,Yikun Jiang,Xiaoyu Yang,Yu Wang,Wei Ji,Guoliang Jia
Purpose Insufficient biological activity heavily restricts the application and development of biodegradable bone implants. Functional modification of bone implants is critical to improve osseointegration and bone regeneration. Methods In this study, L-lysine functionalized graphene oxide (Lys-g-GO) nanoparticles and polydopamine-assisted gold nanoparticle (AuNPs-PDA) coatings were applied to improve the biological function of PLGA scaffold materials. The effects of Lys-g-GO nanoparticles and AuNPs-PDA functionalized coatings on the physicochemical properties of PLGA scaffolds were detected with scanning electron microscopy (SEM), contact angle measurement, and mechanical testing instruments. In vitro, the effects of composite scaffolds on MC3T3-E1 cell proliferation, adhesion, and osteogenic differentiation were studied. Finally, a radial defect model was used to assess the effect of composite scaffolds on bone defect healing. Results The prepared AuNPs-PDA@PLGA/Lys-g-GO composite scaffolds exhibited excellent mechanical strength, hydrophilicity and antibacterial properties. In vitro, this composite scaffold can significantly improve osteoblast adhesion, proliferation, osteogenic differentiation, calcium deposition, and other cell behaviour. In vivo, this composite scaffold can significantly promote the new bone formation and collagen deposition in the radial defect site and presented good biocompatibility. Conclusion The combination of bioactive nanoparticles and surface coatings shows considerable potential to enhance the osseointegration of bone implants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2021
·
IF 5.268
Zhiying Feng,Junchen Wang,Xin Chen,Jie Liu,Ying Zhu,Xiaoming Yang
Currently, carbon dots (CDs) with the eminent phosphorescence have been extensively concerned owing to their more widespread applications. Herein, we employed metformin as the carbon source and successfully synthesized one kind of room-temperature phosphorescence (RTP) CDs doped with the elements of N, P through a facile microwave method. Significantly, the RTP emission of CDs appeared while the solution was fixed on the filter paper. To be specific, the hydrogen bonds formed between CDs and the filter paper, which led to the restriction of molecular rotations and motions. Again, the non-radiation attenuation rate of the excited triplet state was reduced by rigidifying the whole system, thus inhibiting the non-radiative transitions and boosting their RTP emission CDs. Interestingly, the proposed CDs could serve as the RTP ink and was applied to paint various patterns and prepare CDs-PVA film with both fluorescence and phosphorescence. Moreover, both their fluorescence and phosphorescence of these CDs was obviously enhanced by introducing L -tryptophan, thus establishing an innovative dual-channel detection of L -trp. Besides, the detection mechanism was also explored, and the increased hydroxyl-groups enhanced their fluorescence of CDs through the radiative recombination by L -trp, while the phosphorescence was enhanced by the narrowed the energy gap (∆ E ST ), thus promoting the singlet-to-triplet intersystem crossing (ISC).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 18
·5722
·
IF 4.412
Yijie Lou,Kaxi Yu,Xiajun Wu,Zhaojun Wang,Yusheng Cui,Hanxiao Bao,Jianwei Wang,Xiurong Hu,Yunxi Ji,Guping Tang
Resveratrol (RSV) and polydatin (PD) have been widely used to treat several chronic diseases, such as atherosclerosis, pulmonary fibrosis, and diabetes, among several others. However, their low solubility hinders their further applications. In this work, we show that the solubility of PD can be boosted via its co-crystallization with L-proline (L-Pro). Two different phases of co-crystals, namely the RSV-L-Pro (RSV:L-Pro = 1:2) and PD-L-Pro (PD:L-Pro = 1: 3), have been prepared and characterized. As compared to the pristine RSV and PD, the solubility and dissolution rates of PD-L-Pro in water (pH 7.0) exhibited a 15.8% increase, whereas those of RSV-L-Pro exhibited a 13.8% decrease. A 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay of pristine RSV, PD, RSV-L-Pro, and PD-L-Pro against lung cancer cell line A549 and human embryonic kidney cell line HEK-293 indicated that both compounds showed obvious cytotoxicity against A549, but significantly reduced cytotoxicity against HEK-293, with PD/PD-L-Pro further exhibiting better biological safety than that of RSV/RSV-L-Pro. This work demonstrated that the readily available and biocompatible L-Pro can be a promising adjuvant to optimize the physical and chemical properties of RSV and PD to improve their pharmacokinetics.Keywords:resveratrol;polydatin;L-proline;pharmaceutical co-crystal;crystal structure;solubility and dissolution rate
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2021
·
IF 9.381
Xianglin Pan,Haoyu Wang,Ziming Zheng,Xiao Huang,Linlin Yang,Junxi Liu,Kaiping Wang,Yu Zhang
Ulcerative colitis (UC) is an inflammatory bowel disease that affects the colon and rectum. Although galectin-3 (Gal-3) has been reported to play a proinflammatory role in UC, it is unknown whether pectic polysaccharide , a Gal-3 inhibitor in tumor metastasis, can alleviate UC by inhibiting Gal-3. The aim of this study was to investigate the anti-inflammatory effects and underlying mechanisms of SCLP, a pectic polysaccharide purified from Smilax china L. in our previous work, on dextran sulfate sodium-induced UC in BALB/c mice. The results showed that SCLP could significantly improve symptoms, alleviate histopathological damage and reduce the secretion of inflammatory mediators in mice with UC. Analysis of the anti-colitis mechanisms indicated that SCLP could inhibit the Gal-3/NLRP3 inflammasome/IL-1β pathway by suppressing the expression of Gal-3 and the interaction of Gal-3 and NLRP3 . Our results suggested that SCLP could be a promising candidate for prevention and treatment of UC.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Dong Zhang,Luyao Zhao,Wenjuan Wang,Qian Wang,Jianlei Liu,Yuanyuan Wang,Hui Liu,Bo Shang,Xiaoliang Duan,Hui Sun
In the current study, the changes of non-starch and starch lipids in two rice varieties during storage were investigated based on untargeted lipidomic approach. A total of 395 non-starch lipids and 13 starch lipids were identified in two rice varieties. 171 lipids in Nanjing and 6 lipids in Jianzhen were significantly different in stored rice compared to those in the fresh ones. Bioinformatics analysis on these significantly different lipids revealed that the two rice varieties possessed different lipids metabolic pathways during storage. Our study provides insights into the mechanism of the changes in non-starch and starch lipids in rice during storage.
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN POLYMER JOURNAL
·
2021
·
IF 4.598
Qiaohong Peng,Li Wei,Xiaoyan Zhang,Ying Wu,Khalid Mahmood,Zhengping Liu,Lisha Zhang
Ionic liquids (ILs), instead of the classical organic solvents, have shown great improvements in chemical processes. Herein, Water-insoluble alkyl- and benzyl-substituted imidazolium bis(trifluoromethanesulfonyl)imides as non-volatile and thermostable solvents were employed in the direct polycondensation of l -lactic acid (LLA). The effects of monomer concentration, reaction temperature and miscibility between the ILs and poly( l -lactic acid) (PLLA) were comprehensively investigated. Biodegradable PLLA with weight-average molecular weight as high as 132 kDa was synthesized. Compared to melt-polycondensation of LLA, far higher molecular weight could be achieved via the solution polycondensation of LLA in ILs since the viscosity of the polymer melt could be significantly reduced based on the dilution effect of ILs. Polycondensation kinetics of LLA in bulk and in an IL demonstrated that the IL could accelerate the polycondensation of LLA. Influence of lactide, the main by-product, on the polycondensations of LLA was also studied kinetically.
✓ 已验证
产品关联
DOI
高影响因子
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
·
2021
·
IF 3.228
Jianqing Chen,Bing Shao,Jinliang Wang,Zhiqiang Shen,Honggui Liu,Shu Li
Chlorpyrifos (CPF) is an organophosphate insecticide and can cause cell death of animals. In the study, the common carp were exposed to CPF at 0 μg/L (the control group), 1.16 μg/L (the low dose group), 11.6 μg/L (the medium dose group), and 116 μg/L (the high dose group), respectively. The carp were euthanized at the 30th day and gills were collected immediately. The ultrastructural and histopathological observations showed obvious necrosis characteristics and inflammatory injury in the CPF-treated groups. CPF exposure activated the MAPK pathway, in which the mRNA and protein expressions of extracellular signal-regulated (ERK), p38 MAP kinase (p38), and c-Jun N-terminal kinase (JNK) were increased; the mRNAs and proteins of NF-κB and TNF-α were activated; and the mRNAs and proteins of necroptosis related genes were changed (the mRNA and protein expression of RIPK1 , RIPK3 , MLKL, and FADD were increased and caspase-8 was decreased) with concentration dependency. Taken together, we concluded that CPF exposure activated the MAPK/NF-κB/TNF-α pathway, promoted inflammatory injure and evoked necroptosis in common carp gills. In addition, CPF-induced inflammation and necroptosis was concentration dependency. The toxic effects of CPF on gills provided data for both aquaculture and toxicological studies.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENERGY
·
2021
·
IF 7.147
Jin-Long Cui,Li-Wei Cheng,Jing-Yu Kan,Wei-Xin Pang,Jun-Nan Gu,Kun Li,Ling-Ban Wang,Chang-Yu Sun,Xiao-Hui Wang,Guang-Jin Chen,Xing-Xun Li
The spatial difference of hydrate dissociation by depressurization was investigated in an L-shape hydrate simulator to enhance the exploitation efficiency of natural gas hydrates . Hydrates were found to dissociate slower near the mining well than far from the mining well due to the higher water saturation. The mass transfer rate of methane molecules in the water phase was much slower than that in the gas phase. In both vertical and horizontal directions, pore water migrated from the location far from the mining well to that near the mining well in the hydrate reservoir under the pressure difference. This led to a lower dissociation rate for the hydrate distributed at the location near the mining well, which was different from the results in gas/petroleum reservoirs and numerical simulations. The spatial difference was more pronounced in the vertical direction than in the horizontal direction. Gravity caused more water migration in the vertical direction, which led to more uneven dissociation of the hydrates. Sediments with higher permeability and lower mining pressure could result in more pronounced spatial differences. These findings can provide deep insights into the spatial evolution of multiple fields during the exploitation of natural gas hydrates .
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Yufei Yang,Shudong He,Yi Zhang,Xingjiang Li,Haiyan Liu,Qiming Li,Xiaodong Cao,Yongkang Ye,Hanju Sun
Ethanol protocol, two-step protocol and enzymatic extraction were used to extract prolamins from seven kidney beans ( P. vulgaris L.) compared with Osborne protocol. 70–80% (v/v) ethanol concentrations were beneficial to the extraction, and two-step protocol was conducive to higher prolamin content. Varied protein subunits were observed in electrophoresis profiles, and further differences in composition were identified by LC-MS/MS while potential functions were annotated. Stronger hydrophobicity was found with prolamins enrichment from light speckled kidney bean (LSKB), black speckled kidney bean (BSKB), and red kidney bean (RKB), which was negative correlation with α-helix content based on the principal component analysis (PCA). The crude prolamins from the two kinds of white kidney beans possessed higher sulfhydryl and exhibited better water holding capacity (WHC), while higher oil absorption capacity (OAC) was observed in LSKB, BSKB, and RKB. Compared with zein, kidney bean prolamins could be used as food packaging materials due to hydrophobicity.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）