Marine Drugs
·
2025
·Vol. 23
·期 3
·134
·
IF 5.4
Yanglong Liu,Jiawei Sun,Qihao Yan,Bingjian Wen,Yan Bai,Qishi Che,Hua Cao,Jiao Guo,Zhengquan Su
Alcoholic liver disease (ALD) is a liver disorder resulting from excessive alcohol intake, and currently, there are no therapeutics approved by the FDA for its treatment. This study investigates the protective effects and underlying pharmacological mechanisms of two chitosan oligosaccharides, COST (MW ≤ 1000 Da) and COSM (MW ≤ 3000 Da), in mitigating alcohol-induced liver disease (ALD). In animal models, we evaluated the changes in ALD following treatment with COST and COSM. Histopathological analysis revealed that both COST and COSM interventions mitigated hepatic steatosis and inflammatory infiltration. Additionally, these compounds reduced various markers of liver injury, enhanced antioxidant enzyme levels, and significantly improved liver function. Western blot analysis demonstrated that COSM markedly decreased the expression of the hepatic metabolic enzyme CYP2E1, activated the Keap-1/Nrf-2/HO-1 pathway, and restrained the NF-κB and MAPK pathways. In an in vitro model of alcohol-induced hepatocyte L02 injury, both COST and COSM exhibited protective effects on hepatocytes, corroborating the findings from the animal studies. Collectively, in vivo and in vitro experiments confirmed that COST and COSM can reduce oxidative damage, enhance antioxidant capacity, and ameliorate steatosis and inflammatory damage in the liver, thereby significantly attenuating alcohol-induced injury. Notably, COSM exhibited slightly superior efficacy compared to COST.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Peiyao Wang,Yupeng Lv,Xinhui Hou,Xiaoluan Yang,Qian Tao,Guiying Li
Fluorescent probe with aggregation-induced emission (AIE) property has been widely used because of the advantages of high sensitivity, good selectivity and non-destructive testing. The development of fluorescent probe with good biocompatibility, photostability and biodegradability is of great significance in biomedicine and environmental detection. Herein, a novel type of fluorophore CS-TPE for detection of Fe 3+ and bacteria was prepared by the Schiff base reaction of chitosan (CS) and 4-(1,2,2-triphenylethenyl) benzaldehyde (TPE-CHO). The fluorescence response mechanism of CS-TPE system was investigated by various characterization techniques. CS-TPE had an obvious AIE behavior with strong blue-green emissions at 473 nm and reaches the highest photoluminescence (PL) emission in 90 % H 2 O/ethanol mixtures. CS-TPE fluorescent probe exhibited sensitive quenching response to Fe 3+ , which can be used as a biosensor for detecting the concentration of Fe 3+ with short response time (5 min), low detection limit (0.998 μM) and wide detection range (10–300 μM). Meanwhile, CS-TPE exhibited good antibacterial performance for S. aureus and E. coli . It is expected to realize the real-time fluorescence monitoring of metal ion detection and antibacterial process.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Mingzhu Xie,Jing Zhao,Xiaokai Feng,Xu Gao,Wenwen Cheng,Lingheng Kong,Fei Liang
Conventional chemotherapy is challenged by its inherent limitations. As an emerging drug delivery system, nanoparticles are easily eliminated by the immune system due to their exogenous nature. Moreover, the lack of long-circulation and tumor-targeting greatly limits their application. In our research, to address this problem, the biomimetic nanocarriers combine cell membranes, targeting ligand and nanoparticles into one system. Firstly, an active ester monomer and three terpolymers PMAN containing different proportions of phosphorylcholine, sulfonic acid and active ester group were successfully synthesized. Subsequently, aminated-folate was synthesized and grafted onto the terpolymers to prepare the folate-targeted polymers FA-PMAN. Finally, cell membrane biomimetic and folate-targeted chitosan nanoparticles (FA-PMAN/CS) with average particle size of 100 to 200 nm were prepared through electrostatic interactions. The in vivo blood circulation results showed that the long-circulating properties of PMAN/CS nanoparticles were significantly enhanced, compared with TPP/CS nanoparticle as a control. The half-life extended from 2.71 h to 12.95 h, with five-fold increase. Additionally, the uptake efficiency of FA-PMAN/CS nanoparticles by rat breast cancer cells (MADB-106) was 5–6 times greater than that of nanoparticles without folate. The biological distribution in mice demonstrated that the signal intensity in tumors treated with FA-PMAN/CS nanoparticle was substantially higher than in other organs, indicating that folate on the surface of nanoparticles increased the uptake into cancer cells. This work aims to design more efficient nanocarriers through biomimetic simulation of cell membranes, reduce recognition and clearance by the immune system, prolong blood circulation time and achieve tumor-targeted drug delivery. It is expected to significantly improve efficacy and provide an innovative strategy for clinical treatment.
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ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2024
·
IF 6.2
Wenchao Zhang,Lu Shen,Ruyue Xu,Xue Dong,Shurui Luo,Huajie Gu,Fenju Qin,Hengwei Liu
Microbially induced carbonate precipitation (MICP), as an eco-friendly and promising technology that can transform free metal ions into stable precipitation, has been extensively used in remediation of heavy metal contamination. However, its depressed efficiency of heavy metal elimination remains in question due to the inhibition effect of heavy metal toxicity on bacterial activity. In this work, an efficient, low-cost manganese (Mn) elimination strategy by coupling MICP with chitosan biopolymer as an additive with reduced treatment time was suggested, optimized, and implemented. The influences of chitosan at different concentrations (0.01, 0.05, 0.10, 0.15 and 0.30 %, w/v) on bacterial growth, enzyme activity, Mn removal efficiency and microstructure properties of the resulting precipitation were investigated. Results showed that Mn content was reduced by 94.5 % within 12 h with 0.15 % chitosan addition through adsorption and biomineralization as MnCO 3 (at an initial Mn concentration of 3 mM), demonstrating a two-thirds decrease in remediation time compared to the chitosan-absent system, whereas maximum urease activity increased by ∼50 %. Microstructure analyses indicated that the mineralized precipitates were spherical-shaped MnCO 3 , and a smaller size and more uniform distribution of MnCO 3 is obtained by the regulation of abundant amino and hydroxyl groups in chitosan. These results demonstrate that chitosan accelerates nucleation and tunes the growth of MnCO 3 by providing nucleation sites for mineral formation and alleviating the toxicity of metal ions, which has the potential to upgrade MICP process in a sustainable and effective manner. This work provides a reference for further understanding of the biomineralization regulation mechanism, and gives a new perspective into the application of biopolymer-intensified strategies of MICP technology in heavy metal contamination.
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Marine Drugs
·
2024
·Vol. 22
·期 10
·443
·
IF 4.9
Hengjie Su,Xiaoqi Chen,Linna Mao,Ting Li
The electrospinning of pure chitosan nanofibers is highly sensitive to environmental humidity, which limits their production consistency and applicability. This study investigates the addition of sodium chloride (NaCl) to chitosan solutions to enhance spinnability and mitigate the effigurefects of low humidity. NaCl was incorporated into the electrospun chitosan solution, leading to increased conductivity and decreased viscosity. These modifications improved the electrospinning process. Comparative analyses between chitosan membranes (CM) and sodium-chloride-added chitosan membranes (SCM) revealed no significant differences in chemical structure, mechanical strength, or in vitro cell proliferation. This indicates that the addition of 1% (w/v) NaCl does not adversely affect the fundamental properties of the chitosan membranes. The findings demonstrate that NaCl addition is a viable strategy for producing electrospun chitosan nanofibers in low-humidity environments, maintaining their physicochemical properties while enhancing spinnability.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Huajuan Wang,Tian Shi,Jiale Ma,Shutong Meng,Ziyu Wei,Ying Sun,Hongxun Wang,Min Zhou
In this work, a nanocomposite film, designated as CS/PA, was fabricated by integrating chitosan (CS), porphyrinic porous coordination network (PCN), and silver nanoparticles (AgNPs). PCN modified AgNPs was denoted as PCN-AgNPs (PA). The synthesis of PA was verified through transmission electron microscope, Zeta potential, hydrated particle size, element mapping. Furthermore, varying concentrations of PA (0.3 %, 0.4 %, 0.5 %, and 0.6 % w /w) were integrated into the CS matrix to preparate the CS/PA nanocomposite film. The experimental data demonstrated a significant enhancement ( p < 0.05) in the tensile strength and elongation at break of the nanocomposite films, while water vapor permeability and water solubility significantly decreased (p < 0.05) as the PA content increased. Additionally, the CS/PA film showed good antibacterial properties against both gram-positive ( Staphylococcus aureus ) and gram-negative ( Escherichia coli ) bacteria. Notably, the CS/PA film effectively retarded the spoilage of fresh fish slices. The silver migration from the CS/PA film, measured at 33.02 μg/L, was found to be within the permissible limits set by the USEPA. Consequently, the CS/PA nanocomposite film holds immense promise for applications in food packaging industry.
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RSC Advances
·
2017
·Vol. 7
·期 80
·50920-50927
·
IF 3.108
Kuerbanjiang Rouzi,Abuderixiti Abulikemu,Jie Zhao,Biao Wu
A new chitosan based colorimetric anion receptor bearing nitrophenyl as a chromogenic signal group, and urea moiety as recognition site was designed and synthesized. The receptor structure was characterized by FTIR and 1H NMR. The sensing abilities of the receptor for anions were investigated with UV-vis methods. During the addition of PO43− and F− anions, the sensor responded with a dramatic color change, and no change was observed in the presence of other anions. The UV-vis absorption titration data showed that the receptor shows selective recognition and sensing in DMSO-H2O (1%) for PO43− and F− anions. The Job's curve showed that 3 : 2 stoichiometry complexes were formed between the receptor and the anion.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xueyan Li,Changlong Bi,Yuanfei Wang,Chong Peng,Yun Li,Shuyi Yang,E. Tao
To address the pH limitations of traditional Cr(VI) adsorbents, converting Cr(VI) anions into the more stable Cr(III) cations through a reduction reaction is an effective strategy. Therefore, in this study, gallic acid (GA), with a high redox potential, was selected as the reducing agent, together with chitosan (CS) as the substrate, and sodium alginate (SA) as the gelling agent. A novel SCG composite material was successfully prepared using the sol-gel crosslinking method, which efficiently captured and reduced Cr(VI) while immobilizing Cr(III). At pH = 4, t = 150 min, and T = 30 °C, 1 g/L of SCG can remove 94.7 % of Cr(VI), with a reduction rate of 67.5 %, achieving efficient and stable Cr(VI) removal over a wide pH range. Furthermore, the SCG material demonstrated good pH adaptability (It can still remove 84 % of Cr(VI) at pH = 7) and practical applicability. Comparative experiments and DFT calculation results showed that the introduction of GA not only helped form a more compact network structure but also interacted synergistically with CS, significantly promoting the removal of Cr(VI). In conclusion, the SCG composite material provides a new approach for the treatment of Cr(VI)-contaminated wastewater.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Mengxin Zhao,Yifu Liu,Chunhua Yin
Gold nanorods (AuNR) have received significant attention in tumor thermo-chemotherapy. However, insufficient thermal availability limits the in vivo highly efficient applications of AuNR in photothermal therapy. In this study, we have fabricated N-isopropylacrylamide grafted O -carboxymethyl chitosan nanoparticles (NCMC NPs) with thermo-responsive properties for co-encapsulating AuNR and doxorubicin (DOX), forming AuNR@NCMC/DOX nanocomposites (NCs). As a result of the thermo- and photothermal-responsiveness, AuNR@NCMC/DOX NCs exhibited irreversible aggregation at high temperature and under near-infrared (NIR) irradiation with an increase of size to 3 μm. When AuNR@NCMC/DOX NCs reached tumor sites following intravenous administration, they were located in the tumor vessels under NIR irradiation due to an embolization effect. This response enhanced tumor targeting, on-demand release, and the thermal performance of AuNR@NCMC/DOX NCs. We have observed higher tumor accumulation of DOX and AuNR with subsequent stronger inhibition of tumor growth than that achieved without NIR irradiation. The development of AuNR-based NCs with multiple smart responsivenesses at tumors can provide a promising paradigm for solid tumor treatment via the cooperative effects of photothermal therapy and chemoembolization.
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ACS Applied Polymer Materials
·
2024
·
IF 4.4
Yarui Hou,Xiaohui Wang,Changli Ma,Zeqi Liu,Ran Li,Yiyuan Fan,He Cheng,Bin Yang,Kai Li,Qingye Liu
Crystalline aggregates are widely present in various organisms. Regulation of nanostructures is of vital importance to the revelation of life secrets as well as material fabrication. As a representative, chitosan displays polymorphic structures. In tendon chitosan, four polymer chains are packed into an orthorhombic cell, where the antiparallel neighboring chains are linked by two sets of N2···O6 hydrogen bonds, and water molecules are connected with N2, O3, and O5 as well as O6 atoms via hydrogen bonds to stabilize the hydrated structure. The transition from hydrated to anhydrous allomorphs, however, usually involved high-temperature annealing or multistep concentrated acid hydrolysis, which weakened the sustainability of chitosan. To address these issues, we proposed a more convenient strategy to achieve the polymorphic transitions under mild conditions. By subjecting chitosan microgels (CSM) to normal freezing treatment, the hydrated aggregates could be transferred into the anhydrous form. The X-ray diffraction (XRD) results clearly demonstrated that both the (020) and (200) diffraction peaks centered at 2θ = 10 and 19.8° gradually disappeared, while the (110) peak at 2θ = 14.8° indicated the occurrence of anhydrous crystals. Upon freezing, the water molecules that were inserted into the hydrated crystals were removed. Meanwhile, under the effects of N2···O6 intermolecular hydrogen bonding and hydrophobic association, the adjacent polymer chains reorganized into new microcrystalline domains. Moreover, the CSM particles exhibited a strong tendency for self-association during this process. By introducing CSM into the oil/water mixture system to form a Pickering emulsion, the resultant macroporous materials could be used as the oil absorbent through subsequent lyophilization. This work provides a different way to facilely regulate the microcrystalline structure of chitosan in a controllable manner, facilitating the construction of more functional materials.
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Food Packaging and Shelf Life
·
2024
·
IF 8.5
Mei Bao,Honglei Mu,Ben Niu,Ruiling Liu,Huizhi Chen,Hangjun Chen,Lishu Wang,Yanchao Han,Guannan Wang,Haiyan Gao
2 The bioplastic film polyvinyl alcohol (PVA) /chitosan (CS), i.e., PC, formed by blending PVA with CS, exhibits excellent antioxidant properties and biodegradability. However, it lacks ultraviolet (UV) blocking ability, limiting its capacity to protect adipose-rich foods from photo-oxidation. Lignin possesses remarkable UV shielding ability that inhibits food photo-oxidation and extends shelf-life. However, the dark brown color of lignin negatively impacts the film color and transparency. To address these restrictions, this study incorporated acetylated lignin (ALG) loaded with halloysite nanotubes (HNTs) into conventional PC films (PC-HALG), resulting in a significant reduction in the color intensity of the film by almost 255.3 %, i.e., almost being colorless. The UV-A and UV-B shielding capacities of the PC films were increased by 1347 % and 617 %, respectively. Thus, ALG provided the PC films with excellent UV shielding capacity while reducing their color intensity and maintaining high transparency. Additionally, the loading of HNTS reduced lignin agglomeration, contributing to the improvement in transparency and enhancement of UV shielding effects in PC films. Furthermore, the PC-HALG film retained and even enhanced the biodegradability of the PC film, which exhibited degradability within one week. Freshness preservation experiments confirmed that the oxidation of pecan oil covered with the PC-HALG film under UV irradiation was reduced by 81.3 %–92.2 %. These findings suggest that this bioplastic film can be used in visually displaying food products while preventing their photo-oxidation. Mechanical properties, thermal stability, barrier properties, and antioxidant properties of the developed PC-HALG film were also characterized.
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FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Xuemei Sun,Huiyan Wang,Han Liang,Na Meng,Ninglin zhou
In this study, ZnO nanoparticles (ZnONPs) were loaded on lecithin-montmorillonite (LEC-Mt) and incorporated into chitosan (CS) for purpose of developing novel food packaging films. The performances of CS@ZnONPs/LEC-Mt films were investigated by X-ray diffraction (XRD), Fourier transform infrared spectrum (FTIR), Ultraviolet spectrum (UV), thermogravimetric analysis (TGA), scanning electron microscope (SEM) and energy dispersive X-ray (EDX) spectroscopy. In addition, the water-resistance performance, mechanical properties and antibacterial activity were studied. An appropriate increase of ZnONPs/LEC-Mt contents (3 wt% and below) could achieve a remarkable enhancement in elongation at break (E)and tensile strength (TS). Meanwhile, the moisture vapor transmission rate (MVTR) and moisture content (MC) of the CS@ZnONPs/LEC-Mt films also decreased with the incorporation of ZnONPs/LEC-Mt, which was beneficial for food preservation. CS@ZnONPs/LEC-Mt films manifested lower light transmission, MC (from 35.36% to 26.01%) and MVTR (from 0.2012 g∙m -2 ∙24 h -1 to 0.1717 g∙m -2 ∙24 h -1 ) values than those of CS film. Furthermore, CS@ZnONPs/LEC-Mt films possess a certain antibacterial activity against Escherichia coli and Staphylococcus aureus . Due to its good UV light barrier properties, mechanical properties and antibacterial property, CS@ZnONPs/LEC-Mt films showed its great potential in designing packaging films
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