Cell Death & Disease
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2023
·Vol. 14
·期 2
·1-11
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IF 9
Yao Yuan,Feng Suya,Li Xuejiao,Liu Taohua,Ye Shengying,Ma Long,Man Shuli
Litchi chinensis seed, as a valuable by-product of the subtropical fruit litchi ( Litchi chinensis Sonn.), has been confirmed to be rich in procyanidins (LPC). The anticarcinogenic properties of procyanidins has been primarily attributed to their antioxidant and anti-inflammatory activities. However, there is a comparative paucity of information on if and how LPC inhibits colon cancer. Here, LPC significantly inhibited CT26 colon cancer cells proliferation and metastasis in vivo and in vitro. In CT26 lung metastatic mice, the anti-metastatic effect of LPC relied on its regulation of gut microbiota such as increase of Lachnospiraceae UCG-006, Ruminococcus , and their metabolites such as acetic acid, propionic acid and butyric acid. In addition, LPC significantly inhibited CT26 colon cancer cells metastasis through increasing CD8 + cytotoxic T lymphocytes infiltration and decreasing the number of macrophages. Antibiotics treatment demonstrated that the therapeutic effect of LPC depended on the gut microbiota, which regulated T cells immune response. Taken together, LPC had strong inhibitory effects on colon cancer pulmonary metastasis by triggering gut-lung axis to influence the T cells immune response. Our research provides a novel finding for the utilization of procyanidins in the future, that is, supplementing more fruits and vegetables rich in procyanidins is beneficial to the treatment of colon cancer, or it can be used as an adjuvant drug in clinical anti-tumor immunotherapy.
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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
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2023
·Vol. 73
·期 2
·005648
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IF 2.8
Rashidin Abdugheni,Dan-Hua Li,Yu-Jing Wang,Meng-Xuan Du,Nan Zhou,Chang Liu,Shuang-Jiang Liu
The human gastrointestinal tract is inhabited by various microorganisms, including thousands of bacterial taxa that have yet to be cultured and characterized. In this report, we describe the isolation, cultivation, genotypic and phenotypic characterization and taxonomy of five novel anaerobic bacterial strains that were recovered during the massive cultivation and isolation of gut microbes from human faecal samples. On the basis of the polyphasic taxonomic results, we propose two novel genera and five novel species. They areAcidaminococcus hominissp. nov. (type strain NSJ-142T=CGMCC 1.17903T=KCTC 25346T),Amedibacillus hominissp. nov. (type strain NSJ-176T=CGMCC 1.17933T=KCTC 25355T),Lientehia hominisgen. nov. sp. nov. (type strain NSJ-141T=CGMCC 1.17902T=KCTC 25345T),Merdimmobilis hominisgen. nov. sp. nov. (type strain NSJ-153T=CGMCC 1.17915T=KCTC 25350T) andParaeggerthella hominissp. nov. (type strain NSJ-152T=CGMCC 1.17914T=KCTC 25349T).
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Food & Function
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2022
·Vol. 14
·期 2
·796-809
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IF 6.317
Ni-Na Xie,Cheng-Ying Wu,Qiong Ge,Jing Zhou,Fang Long,Qian Mao,Song-Lin Li,Hong Shen
Ginseng polysaccharides (GPs) have shown gut microbiota-related antitumor effects. However, the relation between their structures and antitumor functions remains unknown. Here, crude polysaccharide (GP-c) and its fractions neutral polysaccharide (GP-n) and pectin (GP-a) were prepared for structure characterization and anti-B16F10 melanoma effect evaluation, and their influence on gut microbiota diversities and short-chain fatty acids (SCFAs) were also analyzed. Spearman correlations among the altered gut microbiota, SCFAs, and antitumor effects were conducted to elucidate the structure–function relationships. It was shown that the structures of GP-c, GP-n, and GP-a varied in monosaccharide composition and molecular weight distribution. GP-n and GP-c showed anti-melanoma effects, whereas GP-a promoted its growth slightly. GP-n and GP-c restored SCFAs levels such as acetic acid and butyric acid; moreover, it improved the gut microbiota ecosystem by upregulating the abundance of Allobaculum and Bifidobacterium. However, the restoration effect of GP-a was weak, or even worse. In addition, these two bacteria were negatively correlated with the tumor weight and related with the altered SCFAs. In conclusion, GP-n is essential for the anti-melanoma effects of GP, and the potential mechanisms might be related with its specific regulation of Allobaculum and Bifidobacterium abundance, and tumor-associated SCFAs levels. The outcomes highlighted here enable a deeper insight into the structure–function relationship of GP and propose new opinions on its antitumor effect.
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FOOD AND CHEMICAL TOXICOLOGY
·
2023
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IF 4.3
Fangfang Min,Jielun Hu,Tao Huang,Yousheng Huang,Shaoping Nie,Tao Xiong,Mingyong Xie
Lactobacillus ( L. ) casei NCU011054 isolated from infant feces has been proven to be a potential probiotic in vitro . The present study aimed to investigate the effects of L. casei NCU011054 on the immune response and gut microbiota in cyclophosphamide (CP)-induced immunosuppression mice. Results indicated that L. casei NCU011054 could increase the levels of mucin (Muc2) and tight junction proteins (ZO-1, occludin and claudin-1). Moreover, L. casei NCU011054 was found to upregulate TLRs/NF-κB pathway (TLR-2, TLR-4, TLR-6, p65 and NF-κB) and two transcription factors (T-bet and GATA-3) mRNA levels, and enhance the number of CD4 + T cells. Th1-related cytokines (IL-12p70, IFN-γ and TNF-α) and Th2-related cytokines (IL-2, IL-4, IL-6 and IL-10) significantly increased after L. casei NCU011054 treatment. More importantly, L. casei NCU011054 increased the ratio of T-bet to GATA-3 and IFN-γ to IL-4. Apart from these, L. casei NCU011054 remodeled gut microbiota and modulated gut metabolites in CP-induced immunosuppressed mice. The correlation analysis showed that Lactobacillus upregulated by L. casei NCU011054 was positively correlated with TLRs/NF-κB pathway, and the ratio of T-bet to GATA-3 and IFN-γ to IL-4. All findings revealed that L. casei NCU011054 could improve intestinal immune dysfunction and modulate Th1/Th2 balance via TLRs/NF-κB pathway in CP-induced immunosuppressed mice.
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FOOD CHEMISTRY
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2023
·
IF 8.8
Xiaoyu Li,Siying An,Zhen Luo,Peng Zhou,Lu Wang,Ru Feng
This study aimed to investigate the modulatory effects of polysaccharides from the hard shells of Juglans regia L. (JRP) on intestinal function and gut microbiota of mice. The results showed that JRP could increase the colonic length and colonic index, and ameliorate the histological characteristics of colon. JRP had a positive effect on immunity of mice by improving immune organ indexes. Owing to enhancement of intestinal peristalsis and increase of colonic fecal moisture by JRP, the defecation time was significantly reduced. After gastrointestinal digestion and absorption, JRP was metabolized by intestinal microorganisms to produce short chain fatty acids, thereby lowering the pH of intestine. Through microbial community analysis, the composition of gut microbiota was modulated by JRP via increasing the abundances of beneficial bacteria and decreasing the richness of harmful bacteria. This study suggests that JRP can be served as an excellent prebiotic to promote intestinal health.
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Journal of Environmental Chemical Engineering
·
2023
·
IF 7.7
Huiling Wu,Huangzhao Wei,Xu Yang,Chengyu Jin,Wenjing Sun,Kai Deng,Xin Rong,Gu Bin,Chenglin Sun
Separation of small-molecule carboxylic acids , especially acetic acid, from wastewater is challenging due to its high-water solubility, acidity, and low concentration. In this work, resin-derived spherical activated carbon (RSAC) was directly prepared from resin spheres through facile pre-oxidation and carbonization processes. RSAC was characterized by SEM, N 2 physisorption , XRD, Raman, element analysis and XPS. RSAC showed high removal rates (> 95.8 %) for a variety of small-molecule carboxylic acids. The initial adsorption capacity of acetic acid over RSAC was 23.4 mg/g and 94 % of the removal rate was retained after five successive adsorption-desorption cycles for 1000 mg/L acetic acid. In addition, RSAC showed a high total organic carbon removal rate (> 80 %) for the high-salinity effluent of catalytic wet air oxidation (CWAO) process. The adsorption of acetic acid over RSAC complied with the pseudo-second-order model and Langmuir model ideally, through Vander Waals force and pore-filling effect. Furtherly, discarded resin microspheres were used to prepare spherical active carbon, which showed 88.2 % removal of acetic acid (1000 mg/L). The benign scheme of “treating wastes by wastes” from economical and environmental aspects was realized.
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CHEMICAL ENGINEERING JOURNAL
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2022
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IF 16.744
Guihua Xu,Qiaoning He,Kexin Huang,Ying Wang,Chun Pu,Ganggang Chang,Xiaoyu Yang
Benefiting from their unique properties including faster mass transfer, more exposed active sites and large molecule recognition, hierarchically porous metal–organic frameworks (HP-MOFs), in the form of ultrasmall nanoparticles (NPs) and having self-assembled interparticle mesopores, have garnered greater attention in recent years that given to typical MOFs. However, controllable synthesis of HP-MOFs on the nanoscale remains a significant challenge. Herein, for the first time, a facile top-down acid etching strategy was developed to fabricate a series of nanosized (∼15 nm) mesoporous MOF, HP-UiO-66-NH 2 (Hf). The sizes of mesopores in this material can be effectively tuned by simply controlling the concentration of propionic acid (PA) utilized in the preparation solution. Interestingly, the generated mesoporous HP-UiO-66-NH 2 (Hf) has excellent stability and can be readily constructed by using PA etching solution that has been recycled at least four times. Remarkably, HP-UiO-66-NH 2 (Hf) displays a high size-selectivity for adsorption of enzymes. Associated with its highly matched mesopore size, the nanocomposite generated by complexation with glucose oxidase (GOD), [email protected] 2 (Hf) has a higher catalytic activity and a significantly enhanced stability (recycled at least 7 times) in promoting oxidation of glucose compared to its MOF counterpart, [email protected] 2 (Hf) and native GOD .
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JOURNAL OF ETHNOPHARMACOLOGY
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2022
·
IF 5.195
Chunlian Liu,Chengcheng Song,Yan Wang,Yangxin Xiao,Zhongshi Zhou,Guosheng Cao,Xiongjie Sun,Yanju Liu
Ethnopharmacological relevance Atractylodes lancea (Thunb.) DC. is a Chinese herb that has been commonly used to treat spleen-deficiency diarrhea (SDD) in China for over a thousand years. However, the underlying mechanism of its antidiarrheal activity is not fully understood. Aim of the study The antidiarrheal effects of the ethanol extract of deep-fried A. lancea rhizome (EEDAR) due to spleen deficiency induced by folium sennae (SE) were determined on the regulation of the short-chain fatty acid (SCFA) metabonomics induced by the intestinal flora . Materials and methods The effects of EEDAR on a SE-induced mouse model of SDD were evaluated by monitoring the animal weight, fecal water content, diarrhea-grade rating, goblet cell loss, and pathological changes in the colon. The expression of inflammatory factors (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, IL-6, IL-10), aquaporins (AQP3, AQP4, and AQP8), and tight junction markers (ZO-1, occludin , claudin-1) in colon tissues were determined using quantitative polymerase chain reaction and western blotting . SCFA metabonomics in the feces of mice treated with EEDAR was evaluated using gas chromatography–mass spectrometry. Furthermore, 16S rDNA sequencing was used to determine the effect of EEDAR on the intestinal flora of SDD mice, and fecal microbiota transplantation (FMT) was used to confirm whether the intestinal flora was essential for the anti-SDD effect of EEDAR. Results Treatment with EEDAR significantly improved the symptoms of mice with SDD by inhibiting the loss of colonic cup cells, alleviating colitis , and promoting the expression of AQPs and tight junction markers. More importantly, the effect of EEDAR on the increase of SCFA content in mice with SDD was closely related to the gut microbiota composition. EEDAR intervention did not significantly improve intestinal inflammation or the barrier of germ-free SDD mice, but FMT was effective. Conclusion EEDAR alleviated SE-induced SDD in mice, as well as the induced SCFA disorder by regulating the imbalance of the intestinal microbiota.
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Frontiers in Pharmacology
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2023
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IF 5.6
Chen Xing-Ling,Cai Ke,Zhang Wen,Su Shu-Lan,Zhao Li-Hui,Qiu Li-Ping,Duan Jin-Ao
Introduction: Bear bile powder (BBP) is widely used in the clinic and has a hypoglycemic effect, but its mechanism is not clear.Methods: In this study, type 2 diabetes mellitus (T2DM) rats induced by a high-sugar and high-fat diet combined with streptozotocin were given BBP, and biochemical indexes, pathological sections, metabonomics, intestinal microbiota (IM) and short-chain fatty acids (SCFAs) were determined.Results: The results showed that BBP could reduce blood glucose, relieve inflammation, insulin resistance, and lipid metabolism disorder, and alleviate tissue damage of the liver, spleen, kidney, and pancreas in T2DM rats. It is worth noting that BBP can reverse the changes in blood and urine metabolites in T2DM rats, which are mainly related to tryptophan metabolism, pentose and glucuronate interconversions, starch and sucrose metabolism, and glycerophospholipid metabolism. In addition, BBP restored IM disorder in T2DM rats, decreased the abundance of Allobaculum, Blautia, Dubosiella, and Anaerostipes, enriched the abundance of Lactobacillus, Romboutsia, UCG-005, and norank_f__Eggerthellaceae, and increased the concentration of SCFAs in intestinal contents.Discussion: These findings suggest that BBP may improve T2DM by regulating multiple metabolic pathways, IM composition, and SCFAs levels.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2023
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IF 8.6
Wenjuan Fan,Hui Chang,Wenju Pang,Yufeng Li,Chuanhai Xiao,Yan Jiang,Zhiqiang Jiang,Guangfu Yin
Designing photocatalysts with excellent morphology from MOF is a promising strategy to improve photocatalytic H 2 evolution. Herein, a three-dimensional (3D) honeycomb-like Zn 0.5 Cd 0.5 S -140 assembled by ultra-thin nanosheets with an average thickness of about 1.5 nm was constructed using a novel 3D metal–organic framework (namely PZH-139) as template. Notably, the micromorphology could be tuned and the balance between conduction band (CB) position and band gap could achieve by adjusting the Zn/Cd molar ratio of Zn x Cd 1-x S. In a series of Zn x Cd 1-x S -140 photocatalysts derived from MOF, Zn 0.5 Cd 0.5 S -140 exhibited the highest hydrogen evolution rate (26.32 mmol h −1 g −1 ) by visible-light-driven with an apparent quantum efficiency (AQE) of 29.92 %, which was 6, 11 and 23 times higher than that of I-Zn 0.5 Cd 0.5 S (irregular morphology Zn 0.5 Cd 0.5 S), S-Zn 0.5 Cd 0.5 S (spherical Zn 0.5 Cd 0.5 S) and B-Zn 0.5 Cd 0.5 S (bulk Zn 0.5 Cd 0.5 S) prepared by traditional non-MOFs derived-methods, respectively. The excellent photocatalytic performance could be attributed to the synergism of the larger specific surface area, the balance between CB position and band gap, rapid carriers transfer and the enhanced light absorption capability. More importantly, this rare strategy for constructing highly efficient ultra-thin nanosheet assembled 3D honeycomb-like Zn 0.5 Cd 0.5 S -140 derived from MOF can flourish the design ideas of photocatalysts with specific excellent morphology.
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Journal of Energy Chemistry
·
2023
·
IF 13.1
Xinghua Zhang,Miaojia Song,Jianguo Liu,Qi Zhang,Lungang Chen,Longlong Ma
In this work, a “cyclopentanone-vanillin” strategy was proposed for the preparation of jet fuel range cycloalkanes from lignocellulose-derived ketones and lignin-derived aldehydes via aldol condensation and hydrodeoxygenation (HDO). Ethanolamine lactate ionic liquid (LAIL) exhibited excellent catalytic activity in the aldol condensation of cyclopentanone and vanillin. Desired mono-condensation and bi-condensation products were obtained with yield of 95.2% at 100 °C. It is found that the synergy effects between amino group of ethanolamine and hydroxyl group of lactic acid play a key role in the aldol condensation. The condensation products were converted into cycloalkanes by HDO over 5%Pd/Nb 2 O 5 catalyst. The density of the obtained HDO products is 0.89 g/cm 3 and the freezing point is lower than −60 °C. These results suggest that the resulted cycloalkanes can be used as additives to improve the density and low-temperature fluidity of the jet fuels.
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FOOD RESEARCH INTERNATIONAL
·
2022
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IF 7.425
Fang Fang,Yuxin He,Jutang Zhao,Yanli Zhang,Chunhua Chen,Huijun He,Quanyong Wu,Mengwei Hu,Shaoping Nie,Mingyong Xie,Jielun Hu
Whole grains (WGs) are considered as the representative sources of dietary fiber (DF). Thermal treatments can change the properties of DF, and potentially affecting the gut microbiota as well as human health. In this study, DF content and in vitro fermentation characteristics of 9 kinds of WGs (highland barley, barley, buckwheat, proso millet, quinoa, sorghum, coix seed, foxtail millet, and oats) after boiling and steaming treatments were compared. It was found that boiling and steaming treatments could both increase DF content in these grains, except for barley and foxtail millet. Processed WGs could regulate beneficial microbial genus, such as Bifidobacterium , Prevotella, Megamona and Megasphaera. Oats, quinoa, highland barley, and buckwheat after boiling treatment can produce more total short-chain fatty acids (SCFAs) than steaming treatment ( p < 0.05), while barley, foxtail millet and coix seed showed opposite results. This study can provide data support for the design of WGs diets and the development of WGs products which are beneficial for gut health.
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