Food Chemistry-X
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2025
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IF 8.2
Xueli Yang,Renyuan Chen,Junhai Wu,Fangqiang Yu,Fengmei Liao,Xiaobo Li,Ye Wang,Cheng Zhao,Hanren Zhang,Xinying Wu
The production process of Jiang-flavored Baijiu is highly elaborate, and the flavor characteristics and quality of the base liquor (BL) exert a substantial impact on the quality of the final product. A total of 130 typical samples from three quality grades within seven fermentation rounds were examined. Based on sensory evaluation, gas chromatography-flame ionization detection (GC-FID) technology was employed to detect 59 principal volatile compounds within the samples. Subsequently, K-means clustering, partial least squares discriminant analysis, and odor activity values were combined to analyze the compound variation trends and key differentiating compounds. The results demonstrated that in the seven fermentation rounds, 10 key differentiating compounds significantly affect the BL quality; in each round, 6 compounds markedly influence the quality level. Among them, the contents of acetic acid, ethyl acetate, lactic acid, and acetaldehyde were correlated with the BL quality grade and have the potential to act as quality indicators.
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ANALYTICA CHIMICA ACTA
·
2024
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IF 5.7
Shiyi Li,Lei Li,Mengran Ma,Meining Xing,Xiaohong Qian,Wantao Ying
Background Histidine phosphorylation (pHis) plays a key role in signal transduction in prokaryotes and regulates tumour initiation and progression in mammals. However, the pHis substrates and their functions are rarely known due to the lack of effective analytical strategies. Results Herein, we provide a strategy for unbiased enrichment and assignment of the pHis peptides. First, the entire procedure was designed under alkaline conditions to maintain the stability of the N–P bond of pHis and high-pH reverse-phase chromatography was used to efficiently separate the pHis peptides. Second, exploiting the coelution benefits of diethyl labelling, the ratios of light- and heavy-labelled peptides were accurately quantified, and the sites of phosphorylated histidine were assigned. Finally, Cu-IDA bead enrichment and data-independent acquisition mass spectrometry analysis were used to improve the coverage of the histidine phosphoproteome. With this novel strategy, 768 and 1125 potential pHis peptides were identified from lysates of E. coli and HeLa cells, respectively. And these values represent the highest coverage of the histidine phosphoproteome for both cell types. Significance These data strongly support the presumption that pHis modifications are widely present in bacteria. The study provides an efficient strategy and can lead to a better understanding of pHis-modified substrates and their biological functions.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2018
·Vol. 98
·期 12
·4733-4741
·
IF 2.379
Chunfeng Liu,Qi Li,Chengtuo Niu,Feiyun Zheng,Yun Zhao
BACKGROUND Acetaldehyde is an important flavor component in beer which is possibly carcinogenic to humans. Owing to the limitations of present detection methods, only free-state acetaldehyde in beers has been focused on, while acetal in beers has hardly been reported so far. RESULTS A sensitive headspace gas chromatography method was developed for the determination of diethylacetal and acetaldehyde in beer. The column DB-23 was chosen with a total run time of 22.5 min. The optimal addition amount of NaCl, equilibrium temperature and equilibrium time were 2.0 g, 70 °C and 30 min respectively. For both diethylacetal and acetaldehyde analyses, the limit of detection was 0.005 mg L −1 with relative standard deviation < 5.5%. The recoveries of acetaldehyde and diethylacetal were 95–110 and 95–115% respectively. The diethylacetal and acetaldehyde average contents in 24 beer products were 11.83 and 4.36 mg L −1 respectively. The Pearson correlation coefficient between diethylacetal and acetaldehyde was the highest (0.963). Both diethylacetal and acetaldehyde contents increased to a peak value after fermentation for 3 days and then decreased to a lower value. During both normal and forced aging storage, the diethylacetal content decreased and the acetaldehyde content increased gradually over time. When beers were forced aged for 4 days, the increased ratio of acetaldehyde could be above 40.00%. CONCLUSION The newly established method can be used to assess acetaldehyde level and flavor quality in beer more scientifically. © 2018 Society of Chemical Industry
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Oncotarget
·
2017
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IF 5.168
Jie Gao,Gao-Ju Wang,Zhao Wang,Na Gao,Jing Li,Yun-Fei Zhang,Jun Zhou,Hong-Xin Zhang,Qiang Wen,Han Jin,Hai-Ling Qiao
Hepatofibrosis, which leads to cirrhosis and eventual hepatocellular carcinoma, is a common response to chronic toxin-mediated liver injury. Nitrosamines are potent hepatotoxic agents that cause necrosis and subsequent fibrosis in the liver as a result of cytochrome P450 2E1 (CYP2E1)-dependent metabolism, which generates toxic metabolites that form adducts with nucleic acids, leading to hepatotoxicity and mutagenesis. Herein, CYP2E1 activity and content were determined in fibrotic liver tissue from patients with hepatocellular carcinoma. The relationship between CYP2E1 innate activity and hepatofibrogenesis was evaluated, the effect of inhibition of CYP2E1 activity on hepatofibrosis was determined in a Sprague-Dawley rat model of diethylnitrosamine-induced hepatofibrosis. The results demonstrated that the CYP2E1 activities in human fibrotic tissues are significantly higher than that in normal liver tissues. In rats treated with diethylnitrosamine, the livers demonstrated various degree of fibrotic changes and collagen deposition in individual rats. The Ishak score, which determines the stage of fibrosis, correlated with CYP2E1 innate activity, with greater fibrosis in rat livers with higher CYP2E1 innate activity. Inhibition of CYP2E1 during diethylnitrosamine treatment decreased hepatofibrosis and there was an inverse correlation between the degree of inhibition and the extent of hepatofibrosis. Therefore, high CYP2E1 activity is a risk factor for hepatofibrogenesis induced by nitrosamines.
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COMBUSTION AND FLAME
·
2018
·
IF 4.494
Xiaoyuan Zhang,Lili Ye,Yuyang Li,Yan Zhang,Chuangchuang Cao,Jiuzhong Yang,Zhongyue Zhou,Zhen Huang,Fei Qi
Acetaldehyde oxidation was investigated in a jet-stirred reactor at temperatures from 460 to 900 K, equivalence ratios from 0.5 to 4.0 and pressures of 710–720 Torr. Reactive intermediates under low-temperature conditions, such as methylperoxy, methylhydroperoxide and ketohydroperoxide were detected using synchrotron vacuum ultraviolet photoionization mass spectrometry (SVUV-PIMS). A kinetic model for acetaldehyde oxidation was constructed by incorporating recent theoretical and modeling progress of acetaldehyde kinetics, as well as the calculated results of H-atom abstraction reactions of acetaldehyde by acetylperoxy, methylperoxy and methoxy in this work. The present model was then comprehensively validated against the measurements in this work and the experimental data from literature. Modeling analysis reveals that the main chain-branching pathways of acetaldehyde under low-temperature oxidation conditions are the decomposition of acetylhydroperoxide and methylhydroperoxide. The second O 2 -addition process was verified to exist in the low-temperature oxidation of acetaldehyde as ketohydroperoxide was observed, while its contribution to the overall reactivity was found to be minor under present investigated conditions. On the other hand, the sub-mechanism of acetylperoxy is crucial in the low-temperature regime while the methyl oxidation mechanism dominates in the negative temperature coefficient (NTC) region at all equivalence ratios investigated in this work.
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MOLECULES
·
2024
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IF 4.2
Jiming Zhang,Hui Lin,Chaosong Zheng,Bin Yang,Miao Liang,Yi Lin,Liaoyuan Zhang
2,3-butanediol (2,3-BD) is a versatile bio-based platform chemical. An artificial four-enzyme synthetic biosystem composed of ethanol dehydrogenase, NADH oxidase, formolase and 2,3-butanediol dehydrogenase was designed for upgrading ethanol to 2,3-BD in our previous study. However, a key challenge in developing in vitro enzymatic systems for 2,3-BD synthesis is the relatively sluggish catalytic efficiency of formolase, which catalyzes the rate-limiting step in such systems. Herein, this study reports how engineering the tunnel and substrate binding pocket of FLS improved its catalytic performance. A series of single-point and combinatorial variants were successfully obtained which displayed both higher catalytic efficiency and better substrate tolerance than wild-type FLS. Subsequently, a cell-free biosystem based on the FLS:I28V/L482E enzyme was implemented for upgrading ethanol to 2,3-BD. Ultimately, this system achieved efficient production of 2,3-BD from ethanol by the fed-batch method, reaching a concentration of 1.39 M (124.83 g/L) of the product and providing both excellent productivity and yield values of 5.94 g/L/h and 92.7%, respectively. Taken together, this modified enzymatic catalysis system provides a highly promising alternative approach for sustainable and cost-competitive production of 2,3-BD.
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ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
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2024
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IF 3.8
Yan-Fei Liang,Ze-Xin Niu,Zi-Wen Wu,Qing-Yang Zhang,Xin-Yi Zhao,Lei-Lei Chao,Heng Li,Wen-Yun Gao
Acetolactate synthase (ALS) is an essential enzyme involved in the biosynthesis of platform chemicals acetoin and 2,3-butanediol in several microorganisms. In this study, we investigated the catalytic differences among three bacterial ALSs involved in the ligation of two molecules of pyruvate or 2-ketobutyrate. Based on the findings, we predicted three amino acid residues in each enzyme that caused a discrepancy in accordance with the multi-sequence alignment and molecular docking experiments: I398, A402, and T480 in Bacillus subtilis ALS; V400, Y404, and S482 in Listeria seleigeri serovar 1/2b ALS; and M394, H398, and G476 in Klebsiella pneumoniae ALS. Subsequently, we mutually mutated the residues in the three ALSs. The data obtained confirmed our inference that these three residues in each enzyme are truly correlated with substrate recognition, particularly in recognizing compounds that are larger than pyruvate, such as 2-ketobutyrate, benzaldehyde, and nitrosobenzene. This study further clarifies the biochemical traits of ALSs derived from various bacteria and expands the scope of ALS research.
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MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Yao Jin,Xiangwei Yuan,Junchun Guo,Li Xu,Qian Wang,Xiaoli Xiong
Dopamine (DA) is a central neurotransmitter that plays a crucial role in human metabolism. Its precise detection is of great importance in disease diagnosis. Compared with single-signal detection methods, colorimetric and electrochemical dual-signal outputs can self-validate the measured data and counteract interference effects, which is expected to provide more reliable detection results. In this paper, carbon nanofiber-supported 3D bristlegrass-like π-conjugated conducting metal–organic frameworks ( c MOF) Cu 3 (HHTP) 2 nanorod arrays (Cu 3 (HHTP) 2 NRAs/CNF) catalyst with core–shell structure are synthesized by electrospinning and solvothermal reaction. The obtained catalyst possesses remarkable peroxidase-like activity which can catalyze the colorless 3,3′,5,5′ −tetramethylbenzidine (TMB) to blue oxTMB. The mechanism study showes that ·OH radicals are the main active substances. Meanwhile, the core–shell structure between π-conjugated cMOF and CNF can effectively inhibit the aggregation of Cu 3 (HHTP) 2 nanorods and generate more catalytic active sites, resulting in strong electrocatalytic activity of Cu 3 (HHTP) 2 NRAs/CNF. As a result, a novel dual-mode sensor based on colorimetric and electrochemical are constructed for detecting dopamine (DA) with a good linear relationship ranging from 2.5-60 μM and 0.1–106 μM, and the limit of detection (LOD) are 0.27 μM and 10.1 nM (S/N=3), respectively. This study opens up a new idea for the application of c MOF peroxidase-like enzyme in sensing field.
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Nature Communications
·
2025
·Vol. 16
·期 1
·1-11
·
IF 15.7
Chi Haoyuan,Liang Zhanpeng,Kuang Siyu,Jin Yaxin,Li Minglu,Yan Tianxiang,Lin Jianlong,Wang Shuangyin,Zhang Sheng,Ma Xinbin
Ethylene glycol, a widely used chemical, has a large global capacity exceeding 40 million tons per year. Nevertheless, its production is heavily reliant on fossil fuels, resulting in substantial CO 2 emissions. Herein, we report an approach for electrochemically producing ethylene glycol from biomass glycerol. This process involves glycerol electrooxidation to glycolaldehyde at anode, which is subsequently electro-reduced to ethylene glycol at cathode. While the anode reaction has been reported, the cathode reaction remains a challenge. An electrodeposited electrode with metallic Cu catalyst enables us to achieve glycolaldehyde-to-ethylene glycol conversion with an exceptional faradaic efficiency of about 80%. Experimental and theoretical studies reveal that metallic Cu catalyst facilitates the C=O activation, promoting glycolaldehyde hydrogenation into ethylene glycol. We further assemble a zero-gap electrolyzer and demonstrate ethylene glycol electrosynthesis from glycerol to give a decent production rate of 1.32 mmol cm –2 h –1 under a 3.48 V cell voltage. The carbon intensity assessment based on a valid assumption reveals that our strategy may reduce CO 2 emissions by over 80 million tons annually compared to conventional fossil fuel routes.
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MOLECULES
·
2024
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IF 4.2
Qianchun Zhang,Xixi Long,Shan Tang,Li Jiang,Zhaoru Ban,Yanju Chen,Runkun Zhang
A simple, rapid, and reliable method for detecting harmful gases is urgently required in environmental security fields. In this study, a highly effective cataluminescence sensor based on SiO 2 /MIL-53(Al) composites was developed to detect trace isobutylaldehyde. The sensor was designed using isobutylaldehyde to generate an interesting cataluminescence phenomenon in SiO 2 /MIL-53(Al). Under optimized conditions, a positive linear relationship was observed between the signal intensity of the cataluminescence and isobutylaldehyde concentration. The isobutylaldehyde concentration range of 1.55–310 ppm responded well to the sensing test, with an excellent correlation coefficient of 0.9996. The minimum detectable concentration signal-to-noise ratio ( S/N = 3) was found to be 0.49 ppm. In addition, the sensor was effectively utilized for analyzing trace isobutylaldehyde; the analysis resulted in recoveries ranging from 83.4% to 105%, with relative standard deviations ( RSDs ) of 4.8% to 9.4%. Furthermore, the mechanism of cataluminescence between SiO 2 /MIL-53(Al) and isobutylaldehyde was explored using GC–MS analysis and density functional theory. We expect that this cataluminescence methodology will provide an approach for the environmental monitoring of isobutylaldehyde.
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TALANTA
·
2024
·
IF 5.6
Hao-Jie Bai,Hong-Wei Li,Yi Li,Zhijiu Huang,Sha Liu,Xin-He Duan,Yuqing Wu
Detecting the furfural concentration in Baijiu can be used to assess the quality of Baijiu , allowing for the optimization of processing techniques and the enhancement of overall quality. In this paper, a fluorescence-enhanced method based on carbon dots ( o -CDs) is developed for the furfural determination in Chinese Baijiu . In an environment full-filled with · SO 4 ‐ and · OH , furfural undergone a direct surface reaction with the ortho -diamino groups at o -CDs. The created furan-based imidazole increased the surface electron density, leading an emission enhancement and color changes from orange to green. Thereby, a linear fluorescence response of o -CDs-TA to furfural is established in water with a detection limit of 30.5 nM. Finally, after ethanol correction it is used to determine furfural in Chinese Baijiu with high precision and reproducibility, providing a new strategy with low-cost and high sensitivity. In particular, the idea of covalently connecting target molecule to the CDs surface via the assistance of free radical opens a new avenue to merge the nanoscale and molecular realms through implementing chemical role into carbon nanostructures.
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ANALYTICA CHIMICA ACTA
·
2025
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IF 6
Xuhui Xia,Peng Zhao,Jia Zheng,Xuheng Li,Xin Zeng,Dianhui Men,Yiyao Luo,Changjun Hou,Danqun Huo
Background Discriminating the quality of baijiu is critical for fostering the growth of the China baijiu market and safeguarding customers' rights. However, establishing a small-scale and rapid baijiu discriminating sensor assay still remains a challenge. Results Here, we first introduced ratiometric fluorescence sensor array for the detection of reducing substances in baijiu to achieve baijiu discrimination. A ratiometric fluorescence sensor array is built using 2,3-diaminophenazine (oxidized-state OPD, oxOPD) to quench three distinct fluorescence signals of quantum dots while reducing interference from background signals. The reducing chemicals in baijiu can react with Ag + , weakening the quenching effect and changing the ratio. The discriminating of 12 types of organic small molecules which were presented in baijiu was achieved with 97.2 % accuracy by using machine learning classification methods. Meanwhile, 0.1 μM limit of detection (LOD) for ascorbic acid shows that our methods have the potential to quantitative detect reducing substances. In real sample detection, our methods can discriminate 10 distinct qualities of baijiu with 100 % accuracy. We also encoded the fingerprints of different varieties of baijiu for quality control and information reading. Significance and novelty Overall, our easy but robust sensing array not only overcomes the problem of background signal interference but also gives an ideal way for discriminating different qualities of baijiu, food and other areas.
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