PLANT PHYSIOLOGY AND BIOCHEMISTRY
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2024
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IF 6.1
Siyue Liu,Jinge Xie,Wenqi Luan,Chen Liu,Xiwen Chen,Defu Chen
The current trend in agricultural development is the establishment of sustainable agricultural systems. This involves utilizing and implementing eco-friendly biofertilizers and biocontrol agents as alternatives to conventional fertilizers and pesticides. A plant growth-promoting fungal strain, that could alter root system architecture and promote the growth of Arabidopsis seedlings in a non-contact manner by releasing volatile organic compounds (VOCs) was isolated in this study. 26S rDNA sequencing revealed that the strain was a yeast-like fungus, Papiliotrema flavescens . Analysis of plant growth-promoting traits revealed that the fungus could produce indole-3-acetic acid and ammonia and fix nitrogen. Transcriptome analysis in combination with inhibitor experiments revealed that P . flavescens VOCs triggered metabolic alterations, promoted auxin accumulation and distribution in the roots, and coordinated ethylene signaling, thus inhibiting primary root elongation and inducing lateral root formation in Arabidopsis . Additionally, transcriptome analysis and fungal infection experiments confirmed that pretreatment with P . flavescens stimulated the defense response of Arabidopsis to boost its resistance to the pathogenic fungus Botrytis cinerea . Solid-phase microextraction, which was followed by gas chromatography-mass spectrometry analysis, identified three VOCs (acetoin, naphthalene and indole) with significant plant growth-promoting attributes. Their roles were confirmed using further pharmacological experiments and upregulated expression of auxin- and ethylene-related genes. Our study serves as an essential reference for utilizing P . flavescens as a potential biological fertilizer and biocontrol agent.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2025
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IF 6.3
Yaoyang Xia,Hao Xiang,Fu Wan,Ruiqi Liu,Kaida Hu,Yujie Zheng,Quan Zhou,Weigen Chen
Aqueous zinc ion batteries (AZIBs) with MnO 2 as the positive electrode have attracted extensive attention due to their high theoretical energy density. However, the dissolution of the positive electrode and the poor conductivity during the cycling process affect its development prospects. In this paper, δ-MnO 2 with larger interlayer spacing is adopted, and pre-intercalation of Ni 2+ is used to further expand its interlayer spacing to enhance the diffusion kinetics of Zn 2+ in the cathode. Density functional theory (DFT) calculations show that the charge distribution around Mn and O atoms will be changed to improve the conductivity of the MnO 2 cathode by the Ni 2+ intercalation. However, the intercalation of metal cations will lead to the production of more Mn 3+ . which will lead to further manganese dissolution. Therefore, we prepared a polyindole (PIn) coating layer with high conductivity and can effectively prevent cathode erosion by in-situ polymerization. The formation of a heterogeneous interface between PIn and MnO 2 surface not only provides more active electrochemical sites, but also enhances the conductivity of the cathode and reduces the occurrence of manganese dissolution, which greatly improves the cycle performance and specific capacity of the cathode. Ni-MnO 2 @PIn delivers a discharge capacity of 385 mAh g −1 at a current density of 0.1 A g −1 , and maintains a capacity of 278 mAh g −1 after 200 cycles at a current density of 0.3 A g −1 , with a capacity retention of 95 %.
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ACS Food Science & Technology
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2024
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IF 2.6
Zhengyi Chen,Laishui Yu,Zhi Zhang,Linjing Su,Yuhao Xiong
Traditional foodborne bacterial detection methods have limitations, driving the need for advanced techniques like surface-enhanced Raman spectroscopy (SERS), known for its high sensitivity, specificity, and nondestructive analysis in food safety. However, SERS faces challenges regarding substrate sensitivity and complex sample preparation. This study presents an innovative photo-driven method for synthesizing surfactant-free anisotropic gold nanoparticles for SERS applications. These gold nanostars, with unique morphology and superior SERS performance, are used for sensitive Escherichia coli (E. coli) detection in complex food samples. Gold nanostar synthesis, controlled by the pH, reaction time, and HAuCl4 concentration, results in enhanced SERS capabilities. For in situ indole separation, a metabolic product of E. coli and the detection target, we introduce a miniaturized array gas membrane separation device. The SERS-based E. coli detection method exhibits remarkable sensitivity, with a 5 cfu/mL limit of detection and reliability within a 100–800 cfu/mL concentration range.
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CHEMICAL ENGINEERING JOURNAL
·
2025
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IF 13.2
Xingyue Yang,Lei Wang,Shijie Zhou,Shilong Zhang,Yongfang Xu,Yujie Bian,Zhaowei Tian,Yusen Yang,Min Wei
Liquid organic hydrogen carriers (LOHC), which involve reversible catalytic hydrogenation/dehydrogenation cycles, have attracted considerable attention. This study investigates Cu/CuAlO x catalysts with unique Cu 0 –Cu + interfacial sites, which are prepared via structural topological transformation of layered double hydroxide (LDH) precursors. The optimized Cu/CuAlO x catalysts exhibit exceptional catalytic activity in 1,2,3,4-tetrahydroquinoline (THQ) dehydrogenation, achieving a complete conversion of THQ within 1.5 h and a turnover frequency (TOF) value amounts to 19.94 h −1 at 150 °C, which stands at the highest echelon compared with previously reported heterogeneous catalysts. Furthermore, the catalyst also exhibits excellent performance in quinoline hydrogenation (yield >99 %) after 6 h at 60 °C, and the TOF value reaches up to 7.01 h −1 . A comprehensive investigation based on XPS, TEM and XAFS verifies the dual active states Cu 0 and Cu + sites coexisting on nanoparticle surfaces. Both experimental studies ( in situ DRIFTS and XPS) and DFT calculations reveal the pivotal role of the Cu 0 − Cu + synergistic effect in dictating catalytic behavior: the Cu + species preferentially adsorb and activate the N − H bonds and C − H bonds in N-heterocycles; Whereas the Cu 0 site facilitates efficient reassociation of surface adsorbed H atoms and product desorption (H 2 ). This work exhibits substantial potential for implementation in hydrogen storage technologies, offering a viable pathway towards efficient LOHC utilization in clean energy systems.
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WATER RESEARCH
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2025
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IF 12.4
Han Li,Jinrui Shi,Tao Fu,Jinwei Liu,Xiangtian Peng,Liangjie Wang,Huifang Sun,Yi Xu,Huazhang Zhao
High-temperature wastewaters can themselves activate peroxydisulfate (PDS) to remove aromatic contaminants via polymerization. This, however, may result in an insufficient carbon source for denitrification during biochemical treatment, and the formed polymers, without a proper reuse method, will be costly to handle as hazardous waste. This study demonstrates that the addition of NaOH can suppress the polymerization of aromatic contaminants, which is observed not only in simulated wastewater but also in actual coking wastewater (ACW). Taking phenol as an example, the formation of phenoxy radical (PhO•) through the reaction between SO 4 •- and phenol is the crucial step for phenol polymerization. The addition of NaOH can convert sulfate radicals (SO 4 •– ) to hydroxyl radicals (HO•), and simultaneously, HO• can quickly consume PhO•. Both processes contribute to the inhibition of phenol polymerization. After treatment with heat/NaOH/PDS, the biodegradability of ACW is significantly enhanced with a relatively low carbon source loss (around 16%). Moreover, Fourier transform-ion cyclotron resonance mass spectrometry analysis indicates that the transformation of polyphenols to highly unsaturated and phenolic compounds is beneficial for the biodegradability improvement of ACW. Therefore, the NaOH/PDS system is an effective way to utilize waste heat and enhance the biodegradability of wastewater.
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JOURNAL OF ORGANIC CHEMISTRY
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2024
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IF 3.3
Xiaobin You,Ying Yao,Pengyutian Liu,Lu Chen,Yubao Xie,Guofeng Li,Liang Hong
The development and utilization of new dienes and dienophiles for the controlled synthesis of isoquinuclidines is highly appealing. Herein, we describe a novel strategy for diastereoselective synthesis of indoline-fused isoquinuclidines via copper-catalyzed dearomative Diels–Alder reaction of cyclic amidines with indoles. This protocol avoids the use of unstable DHPs and activated alkenes, offering a more efficient and selective approach to synthesize isoquinuclidines.
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Food Chemistry-X
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2025
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IF 8.2
Mingming Zhang,Meiqin Li,Fang Wang,Jianxin Chen,Yifan Li,Gensheng Chen,Yuwan Wang,Zhihui Feng,Junfeng Yin
Tea aroma is significantly influenced by hydrogen carbonate (HCO 3 − ) in brewing water. This study investigated the impact of HCO 3 − in water on the aroma of brewed tea infusions using sensory evaluation, headspace solid-phase microextraction, and gas chromatography–mass spectrometry techniques. As the concentration of HCO 3 − in water increased, the purity of tea aroma diminished, while attributes related to cooked flavors and sweetness intensified; this effect is particularly pronounced at high temperatures and prolonged brewing times. HCO 3 − changed the concentration of volatiles, resulting in a significant decrease in concentrations of dimethyl sulfide, β-ionone, and other compounds. Furthermore, the presence of HCO 3 − markedly decreased EGCG content while increasing GCG content in tea infusion; these variations in catechin concentrations were correlated with changes in dimethyl sulfide and β-ionone concentrations. These findings enhance the understanding of flavor chemistry concerning tea and water, provide valuable insights for the scientific selection of tea brewing water.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
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2024
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IF 6
Xufeng Qin,Weifeng Xu,Jiangnan Hu,Yong Dong,Renbo Ding,Shuheng Huang,Zhendong Zhao,Hong Chang,Xiaokun Wang,Shuai Dong
Glioma, a common primary brain tumor, is highly infiltrative and invasive, often leading to drug resistance and recurrence. Therefore, the development of novel therapeutic agents is urgently needed. Pseudellone C is a novel marine triindole alkaloid. Screening of its antiproliferative activity against 55 cell lines revealed its anti-CNS cancer potential. A total of 42 derivatives of Pseudellone C were designed and synthesized, and their inhibitory activities against two human glioma cell lines (U-87MG and LN-229) were evaluated using the CCK-8 assay. Ten derivatives exhibited potent antiproliferative activity with IC 50 values below 10 μmol, which are 18- to 39- fold more potent than Pseudellone C. Among these, derivative 4o demonstrated favorable blood-brain barrier permeability. Mechanistic studies revealed that 4o induces apoptosis primarily by activating the downstream caspase 3 cascade via the TNF/TNFR pathway. Structure-activity relationship correlations were systematically analyzed, and a pharmacophore model for further rational design was constructed.
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Journal of Environmental Chemical Engineering
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2024
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IF 7.4
Xiaowei Liu,Chuang Huang,Hongxia Yu,Yaning Yang,Lulu Ma,Bin Zhao,Tianyang Zhong,Lilan Zhang,Weihua Peng,Wenwen Gong,Yan Ding
Microplastics (MPs) provide a specific habitat for bacterial adhesion and the adsorption of extracellular DNA (eDNA), making them hotspots for the transformation of antibiotic resistance genes (ARGs) in the environment. However, the heterogeneous mechanism of ARG regulation by quorum sensing (QS) in MP biofilms remains unclear. This study demonstrated that QS signals, including C8-HSL, 3-oxo-C10-HSL, and C12-HSL, were detected at ng/L levels in Bacillus subtilis biofilms colonized on MPs. The addition of these three AHLs enhanced natural transformation by 1–2 orders of magnitude in both MP and natural substrate (NS) biofilms, while QS inhibitors decreased transformation frequencies by 2 orders of magnitude. Moreover, transformation frequencies in MP biofilms increased 6.0-fold under QS regulation mediated by AHLs, compared to a 2.5-fold increase in NS biofilms. QS-regulated biofilm formation and EPS secretion were found to be responsible for ARG dissemination in MP biofilms, as indicated by the positive correlation between transformation frequencies and EPS variation. Additionally, mRNA expression analysis revealed that activation and inhibition of the QS system in MP biofilms regulated the expression of natural transformation-related genes, including epsG (regulating EPS secretion) and recO (regulating eDNA transformation). These results highlight the significant influence of the QS system on ARG dissemination through heterogeneous biofilm formation and EPS secretion in MP biofilms. Understanding the effect and mechanism of QS on ARG natural transformation provides new insights into controlling ARG spread by targeting QS in host bacteria within MP biofilms.
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Frontiers in Pharmacology
·
2024
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IF 4.4
Li Lin,Jingchuan Yan,Jin Sun,Jianfeng Zhang,Bo Liao
Objective: The PRAS40 is an essential inhibitory subunit of the mTORC1 complex, which regulates autophagy. It has been suggested that Erxian Decoction (EXD) could treat spinal cord injury (SCI) via the autophagy pathway. However, the mechanism of whether EXD acts through PRAS40 remains unclear. Methods: With the help of immobilized PRAS40, isothermal titration calorimetry (ITC) and molecular docking, the bioactive metabolites in the EXD were screened. To establish in vitro SCI models, PC12 cells were exposed to hydrogen peroxide (H2O2) and then treated with the identified EXD substances. Furthermore, Western blot assay was carried out to identify potential molecular mechanisms involved. For assessing the effect of metabolites in vivo , the SCI model rats were first pretreated with or without the metabolite and then subjected to the immunohistochemistry (IHC) staining, Basso, Beattie & Bresnahan (BBB) locomotor rating scale, and H&E staining. Results: The immobilized PRAS40 isolated indole, 4-nitrophenol, terephthalic acid, palmatine, sinapinaldehyde, and 3-chloroaniline as the potential ligands binding to PRAS40. Furthermore, the association constants of palmatine and indole as 2.84 × 106 M-1 and 3.82 × 105 M-1 were elucidated via ITC due to the drug-like properties of these two metabolites. Molecular docking results also further demonstrated the mechanism of palmatine binding to PRAS40. Western blot analysis of PC12 cells demonstrated that palmatine inhibited the expression of p-mTOR by binding to PRAS40, activating the autophagic flux by markedly increasing LC3. The injection of palmatine (10μM and 20 μM) indicated notably increased BBB scores in the SCI rat model. Additionally, a dose-dependent increase in LC3 was observed by IHC staining. Conclusion: This research proved that EXD comprises PRAS40 antagonists, and the identified metabolite, palmatine, could potentially treat SCI by activating the autophagic flux.
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Catalysts
·
2025
·Vol. 15
·期 9
·888
·
IF 4
Qiongqiong He,Zhaoyang Song,Shaomeng Huang,Ruize Gao,Chao Han,Zhenyong Miao
Heterocyclic compounds in high-salinity wastewater are highly resistant to degradation, posing significant treatment challenges. A hybrid micro-nano bubble Fenton system (FT-MNBs) was developed to enhance Fe2+activation via interfacial effects. The FT-MNBs achieved a significantly higher indole degradation rate (0.0380 min−1) compared with micro and nano bubbles (MNBs) alone (0.0046 min−1) and conventional Fenton (0.01008 min−1). In real coking wastewater with a total dissolved solid (TDS) content of 3.266 g/L, FT-MNBs achieved COD removal efficiencies of 93.42% (initial COD 200 mg/L) and 72.54% (initial COD 10,000 mg/L), demonstrating excellent adaptability and efficiency in treating refractory high-salt organic wastewater. Electron spin resonance confirmed •OH as the main reactive species. Molecular simulations revealed that the MNB interface enhances the adsorption energy of Fe and H2O2, alters the Fe 3d orbital to better overlap with the O–O 2p orbital, and increases electron density—thus promoting O–O bond cleavage and free radical generation. The FT-MNBs not only enhances reaction kinetics but also offer scalability and energy efficiency, showing great potential for advanced industrial wastewater treatment.
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FOOD CONTROL
·
2025
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IF 6.3
Miao Tian,Mingchao Li,Hongxia Tan,Tianying Lu,Lintao Zeng
Timely detection of meat spoilage is essential for ensuring food safety and public health. To enable rapid on-site assessment of meat freshness, we designed a colorimetric probe, BCY-OH, to monitor biogenic amines, which are established biomarkers of meat spoilage. BCY-OH undergoes a distinct color transition from yellow to purple upon interaction with cadaverine, and is characterized by a large Stokes shift (109 nm), a rapid response (16 s), excellent selectivity, and high sensitivity (LOD = 0.18 μM). BCY-OH was further fabricated into portable test strips for detecting gaseous cadaverine. The strips exhibited a gradual color change from yellow to purple upon exposure to increasing cadaverine vapor concentrations (0–54.4 ppm). This colorimetric signal remained stable for ten days and allowed for at least four reuse cycles after regeneration with acidic vapor treatment. Furthermore, an intelligent sensing platform based on smartphone RGB analysis was established to enable real-time freshness assessment of shrimp, pork, and chicken. The proposed method was validated against the standard total volatile basic nitrogen method. Therefore, this work provides a simple, portable, and sustainable tool for food safety screening, which is practical for both consumers and food quality control officers.
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