DYES AND PIGMENTS
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2025
·
IF 4.2
Ziyi Lu,Chang Wang,Hailong Cui,Jinglong Li,Meiyu Zhen,Xiaoqing Xiong
Photodynamic antimicrobial technology is an effective approach for eliminating bacteria. With growing emphasis on personal health, the development of functional textiles with photodynamic antimicrobial properties has emerged as a significant research focus. Here, Eosin Y (EY) was employed as the photosensitizer, and poly(caprolactone) (PCL) served as the substrate. A series of photosensitizer-loaded PCL&EY nanofiber membranes were successfully fabricated using electrospinning. The results demonstrated that these nanofiber membranes exhibited excellent fiber diameter distribution, mechanical properties, and hydrophilicity at a photosensitizer content of 0.034 ± 0.001 wt%. To further optimize the properties of the PCL&EY nanofiber membranes, varying concentrations of bovine serum albumin (BSA) were incorporated into the electrospinning solution to modulate the properties of PCL membrane. Among them, the PCL&15EY&75BSA nanofiber membrane with a BSA concentration of 0.166 ± 0.001 wt% exhibited optimal performance across all evaluated parameters, particularly demonstrating a transformation from hydrophobic to hydrophilic properties. Antimicrobial performance tests revealed that the addition of BSA enhanced the photodynamic antibacterial efficiency of the PCL&EY nanofiber membrane, with the PCL&15EY&75BSA membrane achieving bacteriostatic rates of 95 ± 1 % against Escherichia coli ( E. coli ) and 99 ± 1 % against Staphylococcus aureus ( S. aureus ). To expand their applications, these nanofiber membranes were combined with activated carbon masks, creating functional textiles with both high gas permeability and effective antibacterial properties. Therefore, the strategy of incorporating BSA into the electrospinning solution is an effective approach to improving the performance of nanofiber membrane.
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ADVANCED FUNCTIONAL MATERIALS
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2025
·
IF 19
Ye Tian,Xiaojun Lu,Tingbin Wang,Aiping Liu,Lisha Fan,Huaping Wu
Soft microactuators leveraging biomimetic designs and microfabrication advances show substantial promise for soft robotics, precision sensing, and targeted drug delivery. However, simultaneously achieving rapid deformability with mechanical robustness in such systems remains a significant challenge. Herein, a hybrid microactuator with heterogeneous materials is fabricated via femtosecond laser two-photon polymerization. Incorporating acrylic acid as a functional monomer imparts pH-responsive behavior to the hydrogel, whereas the rigid photoresist ensures structural integrity. Moreover, embedded magnetic nanoparticles enable contactless and precise locomotion under an external magnetic field. As a proof-of-concept, a scorpion-inspired microactuator with dual pH/magnetic responsiveness demonstrates programmable functions including capture, transport, and release. This strategy provides a versatile platform for adaptive microrobots with localized responsiveness and remote motion, paving the way for precision therapeutics and cellular micromanipulation.
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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
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2025
·
IF 8.3
Na Wang,Yibo Zhang,Zelin Meng,Mingyang Song,Heming Zhao,Yang Wang,Shuai Bai,Jianglei Hu,Fengwei Shi,Xia Yang
Addressing the low surface area and high charge recombination of graphitic carbon nitride (g-C 3 N 4 ), this study employs a synergistic carboxylation and MOF-loading strategy. Copper-based MOF (Cu-MOF) was uniformly loaded onto carboxylated spherical g-C 3 N 4 (C 3 N 4 –C) via a solvothermal method, creating a visible-light-responsive C 3 N 4 –C-MOF photocatalyst. Material characterization results indicate that the characteristic peak at 1632 cm −1 in the Fourier Transform Infrared Spectroscopy confirms the successful grafting of 4-carboxybenzoic acid (4-CBA). Carboxylation enhanced hydrophilicity and optimized surface charge, improving reactant adsorption. Photoelectrochemical tests showed significantly boosted visible light absorption, increased photocurrent density, and reduced EIS arc radius, confirming efficient interfacial charge separation. Photocatalytic hydrogen production experiments revealed that under simulated solar irradiation, the hydrogen production rate of C 3 N 4 –C-MOF2.0 reached up to 12.68 mmol g −1 h −1 , representing an approximately 1.68-fold increase compared to the unmodified C 3 N 4 -MOF. This work provides a novel approach for designing MOF/functionalized g-C 3 N 4 composites, advancing solar-driven hydrogen production.
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FASEB JOURNAL
·
2025
·Vol. 39
·期 16
·e70901
·
IF 4.2
Yanping Dai,Xiaoqin Gao
Aging-related decline in male fertility is closely associated with impaired sperm quality and dysregulated autophagy/apoptosis in reproductive tissues. This study elucidates the protective role of the AKT2/mTOR/VEGF axis in mitigating age-induced epididymal dysfunction and sperm deterioration. Utilizing iTRAQ proteomics and transcriptome sequencing in young (6-month) versus aged (18-month) rat models, we identified AKT2 and VEGF as key regulators of reproductive aging. Functional validation revealed that AKT2/mTOR/VEGF activation suppressed oxidative stress, preserved mitochondrial membrane potential, and inhibited autophagy/apoptosis in rat epididymal epithelial cells (REECs, cells lining the epididymis involved in sperm maturation and transport) through downstream targets including Bcl-2, Bax, and LC3-II. Notably, extracellular vesicles (EVs) derived from AKT2-activated REECs enhanced sperm motility and reduced sperm DNA fragmentation through reactive oxygen species (ROS) scavenging and autophagy modulation. Pharmacological inhibition of VEGF or AKT2 exacerbated cellular stress responses, while lentivirus-mediated AKT2 overexpression reversed age-related epididymal damage in vivo. Mechanistically, the pathway crosstalk between mTOR-driven VEGF secretion and EV-mediated intercellular communication emerged as a critical mechanism for maintaining sperm viability. These findings establish AKT2/mTOR/VEGF signaling as a central coordinator of epididymal homeostasis and propose EV-based therapies as promising strategies to counteract male reproductive aging. Graphical The AKT2/mTOR/VEGF axis inhibits oxidative stress in REECs, alleviating autophagy and apoptosis. It promotes sperm activity and motility by secreting EVs, reducing sperm autophagy and apoptosis, and thereby increasing the IVF rate.
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Gels
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2025
·Vol. 11
·期 9
·734
·
IF 5.3
Lihan Rong,Jiajiang Xie,Shigao Zhou,Tianqi Guan,Xinyi Fan,Wenjie Zhi,Rui Zhou,Feng Li,Yuyan Liu,Tingting Tang,Xiang Chen,Liyuan Zhang
The scalable fabrication of hydrogels with high toughness and low hysteresis is critically hindered by oxygen inhibition, which typically produces brittle, highly crosslinked (HC) networks. This study presents an oxygen-tolerant photoinduced electron transfer–reversible addition–fragmentation chain transfer (PET-RAFT) strategy for synthesizing highly entangled (HE) polyacrylamide hydrogels under open-vessel conditions. By optimizing the water-to-monomer ratio (W = 3.9) and introducing lithium chloride (LiCl) for spatial confinement, we achieved a fundamental shift in mechanical performance. The optimized HE hydrogel exhibited a fracture energy of 1.39 MJ/m3and a fracture strain of ~900%, starkly contrasting the brittle failure of the HC control (W = 20, C = 10−2) at ~50% strain. This represents an order-of-magnitude improvement in deformability. Furthermore, the incorporation of 15 wt% LiCl amplified the HE hydrogel’s fracture energy to 2.17 MJ/m3while maintaining its low hysteresis. This method enables the rapid, scalable production of robust, transparent thin films that exhibit dual passive cooling via radiative emission (>89% emissivity) and evaporation, rapid self-healing, and reliable strain sensing at temperatures as low as −20 °C. The synergy of entanglement design and confinement engineering establishes a versatile platform for manufacturing multifunctional hydrogels that vastly outperform their crosslink-dominated predecessors.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Lin Zhu,Mingming Zhang,Boyan Gao,Hong Zhang,Hai Zhang,Yaqiong Zhang,Liangli Lucy Yu
In this work, the paraffin oil was first molecularly distilled into two mineral oil saturated hydrocarbons (MOSH) subfractions with their main carbon number range of C20–30 (84.58%) and C30–50 (89.65%), which were determined using a liquid chromatography–gas chromatography–flame ionization detector (LC-GC-FID) technique. Then, both female and male Fischer 344 (F344) rats were fed with the diets containing one of these two MOSH subfractions at the low (1.5 g/kg) or high dose (15 g/kg) for 6 months. It was found that the exposure of MOSH C20–30 subfraction could result in a significant increase of spleen weight index (4.64 ± 0.18 mg/g for female and 3.79 ± 0.31 mg/g for male rats) (P < 0.05), and irregular shape of white medulla, and the macrophage infiltration in mesenteric lymph node for both female and male rats, especially for the 15 g/kg MOSH C20–30-fed rats. Meanwhile, both MOSH C20–30 and C30–50 subfraction promoted inflammation and oxidative stress and had a negative effect on the immune function in both female and male rats. The metabolomics results further supported these observations, and indicated the down-regulation of cysteine and methionine metabolism, and glutathione metabolism pathway in female and male rats, respectively.
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NATURE METHODS
·
2025
·
IF 32.1
Cao Ruijie,Li Yaning,Zhou Yao,Li Meiqi,Lin Fangrui,Wang Wenyi,Zhang Guoxun,Wang Gang,Jin Boya,Ren Wei,Sun Yu,Zhao Zhifeng,Zhang Wei,Sun Jing,Hou Yiwei,Xu Xinzhu,Hu Jiakui,Shi Wei,Fu Shuang,Liang Qianxi,Lu Yanye,Li Changhui,Zhao Yuxuan,Li Yiming,Kuang Dong,Wu Jiamin,Fei Peng,Qu Junle,Xi Peng
In fluorescence microscopy, a persistent challenge is the defocused background that obscures cellular details and introduces artifacts. Here, we introduce Dark sectioning, a method inspired by natural image dehazing for removing backgrounds that leverages dark channel prior and dual frequency separation to provide single-frame optical sectioning. Unlike denoising or deconvolution, Dark sectioning specifically targets and removes out-of-focus backgrounds, stably improving the signal-to-background ratio by nearly 10 dB and structural similarity index measure of images by approximately tenfold. Dark sectioning was validated using wide-field, confocal, two/three-dimensional structured illumination and one/two-photon microscopy with high-fidelity reconstruction. We further demonstrate its potential to improve the segmentation accuracy in deep tissues, resulting in better recognition of neurons in the mouse brain and accurate assessment of nuclei in prostate lesions or mouse brain sections. Dark sectioning is compatible with many other microscopy modalities, including light-sheet and light-field microscopy, as well as processing algorithms, including deconvolution and super-resolution optical fluctuation imaging.
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AQUATIC TOXICOLOGY
·
2024
·
IF 4.1
Mengting Huang,Yuan Ma,Shunli Che,Longteng Shen,Zhicheng Wan,Shiping Su,Shuquan Ding,Xilei Li
Significant concerns have been raised by the widespread pollutants phoxim (PHO) and nanopolystyrene (NP) in the natural environment. This study evaluated the toxicity effects on the hepatopancreas of Eriocheir sinensis caused by NP and/or PHO at concentrations found in the environment. Subchronic exposure to NP and/or PHO triggered hepatopancreas histological damage within a 21-day exposure period. The NP, PHO, and co-exposure (NPO) groups exhibited fewer blister-like (B) cells, along with the appearance of vacuolation. Furthermore, these exposures induced impairment in the hepatic tubule mucus barrier and mechanical barrier, as evidenced by altered expression of oxidative stress-related genes, mucin-related genes, and TJ-related genes. Additionally, alterations in immunity-related genes and inflammatory cytokine genes expression were observed. The findings showed that hepatopancreas inflammation was caused by both individual and combined exposure to NP and PHO and that the inflammatory response was exacerbated by the co-exposure. The possible pathways of hepatopancreas toxicity were further investigated by transcriptomic analysis. Hepatopancreas inflammation was brought on by subchronic exposure to PHO and co-exposure; this inflammation was exacerbated by co-exposure and was backed by the activation of NF-κB signaling pathway via targeting-related genes. In summary, this research represents the initial documentation, to the best of our understanding of the detrimental effects of exposured to NP and/or PHO at levels found in the environment disrupt the hepatopancreas mucus and mechanical barrier in crustaceans, triggering inflammatory responses. These findings highlight the significance of NP and/or PHO pollution for hepatopancreas health.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Jiahui Wang,Kehan Zhu,Miaomiao Zhang,Qian Zhou,Wen Ji,Zhen Yao,Duxin Li
Carrageenan (CGN) is a typical sulfated polysaccharide widely applied in the food and pharmaceutical industries. Its in vivo behavior plays vital roles in understanding structural and biological functional relationships. The lack of UV chromophores in highly sulfated polysaccharides presents a challenge for their in vivo behavior studies. Therefore, this study aimed to develop a fast and effective quantitative fluorescence method for investigating the pharmacokinetics and tissue distribution of CGN. Fluorescence isothiocyanate labeling of CGN (FCGN) and microplate reader-based measurements were developed and validated to study its pharmacokinetics. These results showed that the FCGN concentration peaked at 3 h, the mean residence time was 36.6 h, and the clearance rate was 0.1 L/h/kg. Most of the FCGN was excreted in the feces, while 9.2 % was excreted in the urine, suggesting absorption and metabolism. The pharmacokinetic parameters indicated that the FCGN was absorbed quickly, eliminated slowly, and could remain in the body for a sustained profile. Moreover, ex vivo imaging and quantification of FCGN in tissues revealed that FCGN accumulated in the liver and kidney. Furthermore, oral administration of CGN or KOs for 14 days led to changes in liver and kidney indices. Histological analysis of significant organs revealed hepatocyte necrosis in the liver, renal tubular vacuolization in the kidney, and incomplete colonic epithelial cells. The KOs had a more significant effect on inflammatory cell infiltration than did CGNs. These in vivo findings laid the foundation for the study and application of CGN in food and pharmaceutical applications.
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Food Frontiers
·
2025
·
IF 6.9
Lin Zhu,Mingming Zhang,Hong Zhang,Hai Zhang,Boyan Gao,Liangli (Lucy) Yu,Yaqiong Zhang
This study investigated the effects of mineral oil saturated hydrocarbon (MOSH) sub-fractions (C 20-30 and C 30-50 ) on the colon of male and female F344 rats and the underlying mechanisms. Due to its specific accumulation in the colon, the C 20-30 sub-fraction, particularly at a high dose (15 g/kg), elicited mucosal epithelial detachment and loss of intestinal glands in both sexes after 6 months of exposure. These pathological changes were accompanied by corresponding shifts in biomarkers indicative of increased inflammation (IL-1β, IL-2, TNF-α), oxidative stress (SOD, MDA), and impaired tight junction function (ZO-1, claudin-1, occludin), potentially mediated by the suppression of the Keap1/Nrf2 signaling pathway. Fecal metabolomics further revealed that exposure to either sub-fraction altered metabolites and metabolic pathways in a sex- and dose-dependent manner. The most significantly enriched differential pathways were cysteine and methionine metabolism in females and glutathione metabolism in males. Collectively, these findings provide new evidence for the colon-specific risks of MOSH sub-fractions.
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FUEL
·
2025
·
IF 7.5
Yuchen Han,Yue Han,Yuxin Liu,Xingmeng Xu,Tingyang Li,Ruiyang Zhao,Jishu Han,Lei Wang
In the field of photocatalytic hydrogen production, the construction of p-n heterojunction was an effective strategy to solve the problem of high electron-hole pair recombination rate. In this paper, CoS 2 /CoMn 2 O 4 p-n heterojunction photocatalysts were successfully synthesized by loading CoS 2 nanoparticles on the surface of CoMn 2 O 4 nanocubes. Due to the strong light absorption performance, abundant active sites, and low photogenerated carrier recombination rate, CoS 2 /CoMn 2 O 4 exhibited significantly enhanced photocatalytic hydrogen production activity. Under light irradiation, the hydrogen production rate of CoS 2 /CoMn 2 O 4 could reach to 17.03 mmol/g within 3 h. This study provided a new idea for designing high-efficiency p-n heterojunction photocatalysts.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-20
·
IF 3.9
Wu Honghan,Huang Jun,Wu Hengpeng,Xu Weikang,Zhong Qian,Song Jiaxiang,Linghu Xitao,Gao Botao,Wa Qingde
Autologous and allogeneic bone grafting is currently the clinical gold standard for the treatment of bone defects; however, it is limited by the scarcity of autologous sources and the risk of secondary trauma, as well as the complications of disease transmission and immune rejection associated with allogeneic grafts. The clinical management of bone defects remains a significant challenge. In this study, we prepared a demineralized bone matrix/gelatin methacrylate composite hydrogel loaded with deferoxamine (GelMA/DBM/DFO) using a freeze-drying method and investigated its properties. Assessments using CCK-8, live-dead fluorescence staining, alkaline phosphatase staining, and Alizarin Red staining indicated that the GelMA/DBM/DFO composite hydrogel demonstrated superior biocompatibility and in vitro osteogenic differentiation capacity compared with the GelMA/DBM composite hydrogel. We established a cranial defect model in Sprague–Dawley (SD) rats and examined peripheral blood indices, micro-computed tomography (Micro-CT), hematoxylin and eosin (HE) staining, Masson’s trichrome staining, and immunohistochemical staining for bone morphogenetic protein-2 (BMP-2) and collagen type I (COL-1). Both hydrogels exhibited good biosafety and the GelMA/DBM/DFO hydrogel showed more effective repair of cranial defects in SD rats. This study provides a novel material for bone-defect repair.
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