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JOURNAL OF FOOD SCIENCE · 2020 ·Vol. 85 ·期 1 ·157-164 · IF 2.479

Investigating the antimicrobial and antibiofilm effects of cinnamaldehyde against Campylobacter spp. using cell surface characteristics

Hwan Hee Yu,Ye Ji Song,Hyung-Seok Yu,Na-Kyoung Lee,Hyun-Dong Paik

Campylobacter species are known as biofilm-forming bacteria in food systems. The aim of this study was to evaluate the antimicrobial and antibiofilm effects of cinnamaldehyde against Campylobacter jejuni and Campylobacter coli isolated from chicken meat. The biofilm-forming C. jejuni and C. coli strains from chicken meat were investigated using minimum inhibitory concentration (MIC) and Campylobacter spp. characteristics. The MIC value was 31.25 µg/mL for the Campylobacter strains tested. Cinnamaldehyde had an inhibition and degradation effect on Campylobacter biofilms at concentrations > 15.63 µg/mL. Campylobacter strains treated with 15.63 µg/mL CA exhibited significantly decreased autoaggregation, motility, exopolysaccharide production, and soluble protein. In addition, Campylobacter biofilms formed on stainless steel were degraded following cinnamaldehyde treatment, as determined by scanning electron microscopy. Taken together, these results suggest that cinnamaldehyde constitutes a potential natural preservative against Campylobacter and a nontoxic biofilm remover that could be applied to control food poisoning in the poultry manufacturing-related food industry. Practical Application Cinnamaldehyde was able to effectively remove the biofilm of Campylobacter in the small crack of stainless steel. Cinnamaldehyde has a potential to replace the synthetic antimicrobial and/or antibiofilm agent as well as has a positive influence on consumer concern for the food safety issues of the poultry industries.

CAS / MDL: 104-55-2
研究领域: 微生物学 · 食品安全 · 食品科学
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FOOD SCIENCE AND BIOTECHNOLOGY · 2015 ·Vol. 24 ·期 4 ·1257-1263 · IF 0.653

Examination of the effects of temperature and pressure on lipoxygenase activities in pork using response surface methodology

Huang Yechuan,Wu Zhaomin,Wang Yanrong,Li Feng

To investigate the effects of pressure and temperature on lipoxygenase (LOX) in meat, changes in LOX activities in minced pork and in crude enzyme extracts under different conditions of pressure (131.82–468.18 MPa), temperature (23.18–56.82°C), and duration (6.59–23.41 min) were evaluated using response surface methodology. LOX activities in extracts exhibited a decrease with an increasing treatment temperature, pressure, or duration. Each of these factors alone had a significant ( p <0.05) effect on LOX activities. Activation of LOX in most minced pork treated at less than 400 MPa was observed. Treatment pressure and temperature, and interactions between pressure and temperature and between duration and pressure, significantly ( p <0.05) affected LOX activities. LOX showed higher stability in muscles than in extracts during combined pressure-heat treatments. The most important factor affecting LOX was treatment temperature for extracts and treatment pressure for muscles.

研究领域: 食品科学 · 生物化学
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CERAMICS INTERNATIONAL · 2013 · IF 1.789

Preparation of high performance AlN/Hydantion composite by gelcasting and infiltration processes

Chao Jiang,Dou Zhang,Xueping Gan,Rui Xie,Fuqiang Zhang,Kechao Zhou

A new processing technique was proposed for preparing aluminum nitride (AlN)/Hydantion interpenetrating network composites. The technique comprised gelcasting AlN green bodies, degreasing, and infiltrating Hydantion epoxy resin and hardener into the AlN skeleton under a vacuum condition. The effects of AlN contents on the viscosities of AlN slurries and the flexural strength , thermal conductivity , dielectric constant of the AlN/Hydantion composites were evaluated. The highest thermal conductivity and flexural strength of the AlN/Hydantion composites reached 3.56   W/mK and 101.3   MPa, respectively. The fracture surface of the composite revealed that an interpenetrating polymer network in the AlN ceramic skeleton was formed. The AlN/Hydantion composite exhibited the dielectric constant of 7.3 at 1   MHz.

CAS / MDL: 9002-98-6
研究领域: 材料科学 · 工程学
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JOM · 2021 ·Vol. 73 ·期 11 ·3451-3459 · IF 2.474

Fabrication and Mechanical Properties of Mo-Al2O3 Cermets by Using Ultrafine Molybdenum and Nano-sized Alumina Powders

Chen Ben,Li Zhi-Bo,Zhang Guo-Hua,Chou Kuo-Chih

To improve the performance of Mo-based cermets, Mo-(Ni)-Al 2 O 3 cermets were prepared with raw materials of nano-sized Al 2 O 3 powder. The effects of Al 2 O 3 and Ni additions on the densification behavior, microstructure, bending strength, and hardness of the Mo-(Ni)-Al 2 O 3 cermets were experimentally analyzed. The results showed the content of Al 2 O 3 had a great influence on the degree of densification of Mo-Al 2 O 3 cermets at the sintering temperature of 1600°C. In addition, the Al 2 O 3 particles were distributed evenly in the matrix of Mo-based cermets, which refined the grains of Mo and increased the hardness of cermets. Because of the weak interfacial bonding strength between Al 2 O 3 ceramic phase and Mo metal phase, the bending strength decreased continuously with increasing Al 2 O 3 content. However, Ni addition improved the wetting and adhesion of Al 2 O 3 and Mo. With the addition of Ni, the relative density increased, and thus hardness and bending strength were improved.

CAS / MDL: 1344-28-1 7440-02-0
研究领域: 材料科学 · 机械工程
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Marine Life Science & Technology · 2021 ·Vol. 4 ·期 1 ·74-87 · IF 0

Design and evaluation of chitosan-amino acid thermosensitive hydrogel

Tong Jianan,Zhou Huiyun,Zhou Jingjing,Chen Yawei,Shi Jing,Zhang Jieke,Liang Xinyu,Du Tianyuan

Chitosan/glycerophosphate thermosensitive hydrogel crosslinked physically was a potential drug delivery carrier; however, long gelation time limits its application. Here, chitosan-amino acid (AA) thermosensitive hydrogels were prepared from chitosan (CS), αβ-glycerophosphate (GP), and l -lysine (Lys) or l -glutamic acid (Glu). The prepared CS-Lys/GP and CS-Glu/GP hydrogel showed good thermosensitivity and could form gels in a short time. The optimal parameters of CS-Lys/GP hydrogel were that the concentration of CS-Lys was 2.5%, the ratio of CS/Lys was 3.5/1.0, the ratio of CS-Lys/GP was 4.5/1.0. The optimal parameters of CS-Glu/GP hydrogel were that the concentration of CS-Glu was 3.0%, the ratio of CS/Glu was 2.0/1.0, and the ratio of CS-Glu/GP was 4.0/1.5. Chitosan-amino acid (CS-AA) thermosensitive hydrogel had a three-dimensional network structure. The addition of model drug tinidazole (TNZ) had no obvious effect on the structure of hydrogel. The results of infrared spectroscopy showed that there were hydrogen bonds between amino acids and chitosan. In vitro release results showed that CS-Lys/GP and CS-Glu/GP thermosensitive hydrogels had sustained release effects. Thus, the chitosan-amino acid thermosensitive hydrogels hold great potential as a sustained release drug delivery system.

CAS / MDL: 19387-91-8
研究领域: 生物材料学 · 药剂学
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RSC Advances · 2022 ·Vol. 12 ·期 33 ·21600-21608 · IF 4.036

Ascorbic acid-induced fiber-scrolling of titanium carbide Ti3C2Tx MXene

Jinxin Cao,Yuru Wang,Bingqing Wei,Jiaxin Ye,Qing Zhang

Changing the morphology of two-dimensional materials often offers an efficient and effective means to exploit their electronic and mechanical properties. Two-dimensional materials such as graphene can be scrolled into one-dimensional fibers via simple sonication. Unfortunately, scrolling MXene nanosheets into fibers is quite challenging, especially Ti3C2Tx composed of three layers of titanium atoms and two layers of carbon atoms. Herein, we report a new method to fabricate MXene fibers via ascorbic acid (AA) induced scrolling of Ti3C2Tx nanosheets. An unusual AA-Ti3C2Tx interaction is discovered in that intercalated AA molecules bind to and interact with the Ti3C2Tx surface in the form of a hydrogen bonding-bonded assembly instead of as individual molecules, and a sheet-scrolling mechanism is proposed based on this interaction. The as-obtained MXene fibers exhibit a compact cross-section, and the diameter can be tailored from hundreds of nanometers to several micrometers through tuning the MXene/AA ratio. Moreover, the storage modulus of the MXene-fiber sponge attains its maximum value of ∼1 MPa when a unique morphology comprising both fibers and not-yet-scrolled sheets is presented. This work offers a new strategy of fiber-shaping MXenes for applications in structural composites and flexible electronics.

CAS / MDL: 144-55-8
研究领域: 材料科学 · 纳米技术
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FOOD CHEMISTRY · 2022 · IF 9.231

Determination of four aflatoxins on dark tea infusions and aflatoxin transfers evaluation during tea brewing

Ting Ye,Hangbin Yan,Xu Wang,Weifan Zhao,Fang Tao,Yu Zhou

In daily tea drinking, little is known on aflatoxin transfers from tea-leaf to infusion during brewing and the actual intake. A verified aflatoxins analytical method on tea infusion is significant to the exposure assessment. Here, an optimal method 3 (M3) was screened and validated on four aflatoxins (B 1 , B 2 , G 1 and G 2 ) simultaneous determination. Recoveries of AFG 1 and AFB 1 were 87.37 %±1.99 %-102.03 %±8.62 %, and AFG 2 and AFB 2 were 83.99 %±8.65 %-100.14 %±1.69 %. The correlation coefficient on the fortified samples determination was > 0.99 for each aflatoxin. In accuracy and precision validation, aflatoxins recoveries in high and low fortified samples were 85.94 %-103.83 %, and RSDs were 2.20 %-9.15 %. Method applicability test showed that the M3 was qualified for six different dark tea types. Extraction rate of 30-days stored AFB 1 fortified tea-leaf by acetonitrile–water (84:16, v/v) was < 50 %; meanwhile, only 2.94 % spiked AFB 1 was released by ten times tea brewing which indicated that Puerh tea have strong adsorbability to aflatoxin.

研究领域: 食品科学 · 分析化学
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Surfaces and Interfaces · 2023 · IF 6.2

FeS@h-BN as an adsorbent for the removal of Se (IV) from solution: Characterization and characteristics in a fixed bed column

Xiangyue Wei,Yixin Pan,Jisheng Kou,Rui Huang

The removal of selenium (IV) from aqueous solutions was the focus of research that led to the discovery of a novel type of composite adsorbent called FeS@h-BN nanocomposite . This adsorbent was studied, characterized, and put to use in a fixed bed column. SEM, FTIR, XRD, and XPS analyses were conducted to learn more about the coated layer's surface properties. The effect of several adsorbent materials on the remediation of Se(IV) in dynamic water was examined. The Adams-Bohart model, the Thomas model, and the Yoon-Nelson model were all utilized to get optimal fits to the kinetic data obtained from the adsorption process. Moreover, the Adams-Bohart model was only applicable to the beginning portion of the dynamic behavior of the FeS and FeS@h-BN columns. The Thomas and Yoon-Nelson models were sufficient for describing the breakthrough curve under all experimental conditions. For this dynamic process, the theoretical breakthrough curve profile was compared to an experimentally derived profile. It was discovered that FeS@h-BN nanocomposites are the most effective at removing Se(IV), and the adsorption of Se(IV) by a variety of adsorbent materials is enough for all three dynamic models.

研究领域: 环境科学 · 材料科学 · 化学工程
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FOOD MICROBIOLOGY · 2025 · IF 4.6

Directed regulation of high-temperature Daqu microbiota and metabolites using synthetic communities

Qiuxiang Tang,Yi Zhang,Jun Huang,Rongqing Zhou

High-temperature Daqu (HTD) plays a critical role in shaping the microbial communities and metabolic profiles during the production of Jiangxiang-flavor Baijiu (JXFB). However, directed regulation of HTD properties remains challenging due to complex interactions among biotic and abiotic factors throughout the process. This study investigated the impact of Bacillus strains, including individual strains and synthetic microbial communities (SynMCs) composed of multiple strains, when combined with Monascus floridanus or Muqu , on the microbiota and metabolite profiles of HTD. Our results demonstrated that both functional strains and their combinations significantly influenced the microbial community structure and key flavor compounds. Bacillus- based interventions significantly increased pyrazine content, particularly in SynMCs supplemented with Muqu . Samples treated with Monascus and Bacillus exhibited greater consistency in organic acids, non-volatiles, and volatiles compared to other treatments, indicating that interkingdom interactions between fungi and bacteria enhance flavor control. The similarity index among fungal communities reached 44.44 %, while bacterial community similarity was only 25.51 %, and cluster analysis revealed a directional pattern in fungal succession. Co-occurrence network analysis indicated that high-yielding metabolite communities exhibited elevated average degree, average path length, and clustering coefficient, suggesting a higher connection density and information transfer, thereby promoting metabolite production. Kroppenstedtia displayed varied functions across different treatments, indicating that specific microbial combinations modulate the ecological roles of key taxa, resulting in changes in community structure and metabolism. These findings provide a theoretical basis for more controlled HTD fermentation, potentially enhancing the quality of JXFB.

CAS / MDL: 103616-89-3 64-18-6
研究领域: 食品微生物学 · 发酵技术 · 合成生物学 · 微生物生态学 · 代谢组学 · 应用微生物学
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MICROCHEMICAL JOURNAL · 2025 · IF 5.1

Detection of Aflatoxin B1 and ochratoxin a with a dual-function photoelectrochemical aptamer sensor based on BiOBr/Bi/BiOI hetero and Ag2S-sensitized signal amplification

Meixin Li,Pengfei Yu,Shanshan Li,Mati Ullah Khan,Shun Wang,Jiaqi Tian,Shuang Liu,Ling Kong,Zhiwei Chen

Aflatoxin B 1 (AFB 1 ) and ochratoxin A (OTA) are highly toxic mycotoxins prevalent in food and feed, posing severe threats to human and animal health. This work presents an innovative photoelectrochemical aptamer sensor for the detection of AFB 1 and OTA, leveraging a ternary BiOBr/Bi/BiOI heterojunction and Ag 2 S quantum dot-mediated signal amplification. The BiOBr/Bi/BiOI composite was synthesized via a one-step hydrothermal reduction using l -ascorbic acid (AA), exhibiting a high specific surface area to enhance biorecognition efficiency. In situ-assembled Ag 2 S quantum dots amplified photocurrent responses, while a single aptamer probe enabled specific dual-target recognition. Under optimized conditions, the sensor achieved exceptional sensitivity: linear ranges of 0.001–100 ng·mL −1 for both mycotoxins, with detection limits of 4.34 fg·mL −1 (AFB 1 ) and 13.54 fg·mL −1 (OTA). Validation in complex food matrices confirmed high accuracy and robustness. This platform not only provides a reliable method for mycotoxin monitoring but also establishes a versatile design paradigm for multiplexed detection of food contaminants.

研究领域: 分析化学 · 食品科学 · 生物传感器 · 纳米技术 · 环境监测
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY · 2024 · IF 5.7

Synthesis, Characterization, and Antifungal Activity of Benzimidazole-Grafted Chitosan against Aspergillus flavus

Jing Liu,Hao Chen,Yan Lv,Huixiang Wu,Li-jun Yang,Jiahui Zhang,Jianying Huang,Wenjie Wang

Aspergillus flavus contamination in agriculture and food industries poses threats to human health, leading to a requirement of a safe and effective method to control fungal contamination. Chitosan-based nitrogen-containing derivatives have attracted much attention due to their safety and enhanced antimicrobial applications. Herein, a new benzimidazole-grafted chitosan (BAC) was synthesized by linking the chitosan (CS) with a simple benzimidazole compound, 2-benzimidazolepropionic acid (BA). The characterization of BAC was confirmed by Fourier transform infrared (FTIR) spectroscopy and nuclear magnetic resonance spectroscopy (1H and 13C NMR). Then, the efficiency of BAC against A. flavus ACCC 32656 was investigated in terms of spore germination, mycelial growth, and aflatoxin production. BAC showed a much better antifungal effect than CS and BA. The minimum inhibitory concentration (MIC) value was 1.25 mg/mL for BAC, while the highest solubility of CS (16.0 mg/mL) or BA (4.0 mg/mL) could not completely inhibit the growth of A. flavus. Furthermore, results showed that BAC inhibited spore germination and elongation by affecting ergosterol biosynthesis and the cell membrane integrity, leading to the permeabilization of the plasma membrane and leakage of intracellular content. The production of aflatoxin was also inhibited when treated with BAC. These findings indicate that benzimidazole-derived natural CS has the potential to be used as an ideal antifungal agent for food preservation.

CAS / MDL: 23249-97-0 7585-39-9
研究领域: 材料科学 · 微生物学 · 食品科学
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INDUSTRIAL CROPS AND PRODUCTS · 2024 · IF 5.6

Mechanism study on Monarda didyma essential oil inhibiting Aspergillus flavus infection and aflatoxins accumulation in peanuts

Chenggang Song,Wuliang Shi,Jian Yang,Gang Ding,Wei Chen,Chengguo Jia,Mingzhe Zhang,Jianchun Qin

The presence of aflatoxins resulting from Aspergillus flavus infection in food poses a significant threat to human health due to their potent carcinogenicity or hepatotoxicity. Monarda didyma essential oil, with 92.3% thymol as the active ingredient, is effective in inhibiting A. flavus infestation during peanut storage. Thymol was found to inhibit the growth of A. flavus with an IC 50 value of 14.5 µg mL −1 . When exposed to thymol stress, A. flavus experiences disruption of its glycolysis and tricarboxylic acid cycle, leading to the accumulation of reactive oxygen species and resulting in cell membrane damage and apoptosis. Furthermore, thymol leads to the downregulation of multiple genes, including aflA , involved in the aflatoxin biosynthesis, resulting in a reduction in the production of aflatoxins. This demonstrates the potential of M. didyma essential oil and thymol as effective inhibitors of mycotoxins, thereby contributing to the resolution of food safety issues.

CAS / MDL: 89-83-8
研究领域: 食品科学 · 微生物学 · 药理学 · 真菌学 · 植物化学
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