INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Shuwei Yuan,Miaomiao Zhang,Zhen Yao,Jiangyun Liu,Xiang Li,Zhenqing Zhang,Duxin Li
The swollen culm (also known as Jiaobai) of Zizania latifolia is formed by the smut Ustilago esculenta invades the Z. latifolia . The new tissue formed due to the symbiotic relationship has entices the attention of researchers to study its polysaccharide structure along with biological evaluation. Five fractions of polysaccharides were obtained owing to hot water extraction, alcoholic precipitation, and chromatographic purification. Bioactivity assays showed that ZLPs have good antioxidant, hypoglycemic activities and protective activity against oxidative damage. The ZLP-1 and ZLP-2 were determined to be neutral polysaccharides with high purity, exhibiting propitious bioactivity, consequently they were subjected to indispensable structural characterization. These results showed that ZLP-1 has molecular weight (Mw) of 103 kDa and glucose (Glc) (76.68 %) as the primary monosaccharide; the ZLP-2 has Mw of 122 kDa and galactose (Gal) (41.04 %) and arabinose (Ara) (27.12 %). Structural elucidation by methylation and nuclear magnetic resonance (NMR) analysis suggested ZLP-1 is a glucan, with →3)-β-Glc p -(1→3)-β-Glc p -(1→4)-β-Glc p -(1→4)-β-Glc p -(1→3,6)-β-Gal p -(1→3)-β-Glc p -(1→ as the mainchain and the terminal Ara f and Glc p ; the ZLP-2 is a Galactoxylan, with →3,4)-β-xyl p -(1→3)-β-Gal p -(1→3,6)-β-Gal p -(1→3,6)-β-Gal p -(1→ as the mainchain and the terminal Ara f and Glc p . The structural arrangements provide a chemical basis for understanding the nutritional and pharmacological activities of polysaccharides from Zizania latifolia .
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CHEMICAL ENGINEERING SCIENCE
·
2023
·
IF 4.7
Qingqing Zhu,Huixian Dong,Dongxia Yan,Die Gao,Kaixin Xu,Xiujie Cheng,Jiayu Xin,Xingmei Lu
The transformation of lignocellulose into bioethanol is of great significance for energy security and environmental protection. Traditionally, the concentration of ethanol obtained by fermentation does not exceed 7 % (v/v), resulting in high cost of rectification (about 100 t water are needed to produce 1 t ethanol) and a large amount of wastewater, which is not suitable for industrial production. In this study, producting high concentration bioethanol from ionic liquid pretreated corn stalk integrated by feed-batch method was proposed. The cellulose content and the lignin removal rate of the corn stalk pretreated by [BHEM]mesy-ethylene glycol co-solvent system (rich-cellulose material) were 91 % and 93 %, respectively. The co-solvent system showed stable pretreatment efficiency after amplification of 20 times. Observably, the rich-cellulose material became easier to enzymolysis because the lower crystallinity from XRD, less lignin from CLSM and so on. The optimal enzymolysis conditions were 160 EGU/g cellulose, 50 ℃, pH = 4.8, 96 h, under which the yield of glucose could reach 90 %. The tolerance limit of Saccharomyces cerevisiae to glucose concentration is 0.3 g/L. The viscosity of enzymolysis materials was one of the main factors limiting the efficiency of enzymolysis by rheometer, and the 5 % concentration of materials was the most suitable for enzymolysis. Significantly, by feed-batch method, the glucose concentration reached 250 g/L with the yield of 100 %. The ethanol concentration is 93 g/L with the yield of 73 %. This study provided the possibility for industrialization of bioethanol production.
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Arabian Journal of Chemistry
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2023
·
IF 6
Xin-Yang Jiang,Cai-Wei Wang,Jun Zhang,Pei-Pei Xu,Yi-Ting Xue,Qian Wang
The tea of fig ( Ficus carica L. ) has long been used as a medicine in China due to its excellent effects on hemorrhoids, damp heat diarrhea and other diseases. The present study was designed to evaluate the impacts of different extraction methods, including hot water extraction (HWE), microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), ultrasound assisted enzymatic extraction (UAEE), ultrasound-microwave assisted extraction (UMAE), alkali extraction (AE) and high-speed shearing homogeneous extraction (HSHE), on physicochemical characteristics and bioactivities of polysaccharide from fig leaves (FLPs). Results indicated that there were great differences between the structure and activity of FLPs prepared by seven methods. Among them, FLPs extracted by UAE (FLPs-UAE) showed the strongest antioxidant and hypoglycemic activities due to its higher Mw, total polysaccharide content (60.62±3.06%) and Glucosamine (GlcN) content (7.68%). In addition, FLPs extracted by HWE possessed more total polysaccharide content (62.03±0.70%), total phenolic compounds content (13.55 ± 0.02 %) and GlcN content (5.78%) contributed to its strong antioxidant activity and the inhibition on α-amylase and α-glucosidase, which was second to FLPs-UAE. These finding suggested that UAE and HWE, especially UAE, were good ways to extract FLPs with high antioxidant and anti-diabetic activities, which provide foundations for choosing the suitable extraction strategy to prepare FLPs with desired bioactivities and promoting the application of FLPs in function food industries.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
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2023
·Vol. 25
·期 45
·31444-31456
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IF 3.3
Yiwei Zhu,Jian Kang,Die Gao,Bingtong Chen,Yi Nie,Hui Wang,Xiaowen Wu
Ionic liquid based technology is promising in the pretreatment of lignocelluloses. More efforts are still being made to intensify the separation of the main components in this biomass and to inhibit biopolymer degradation, especially in the fabrication of functional materials where excellent mechanical properties are often requisite. In this study, additives with amino and/or hydroxyl groups were proposed to improve the dissolution of lignocellulosic biomass in ionic liquids and to inhibit the degradation of cellulose. Among the tested additives (i.e., urea, L-2-aminobutyric acid, DL-aminopropanol, 3-aminopropanol and ethanolamine), 3-aminopropanol showed the best performance in enhancing wheat straw dissolution and cellulose recovery in 1-ethyl-3-methylimidazolium acetate ([EMIM]Ac). Further study revealed that this additive could also inhibit cellulose degradation in [EMIM]Ac. The interactions between the ionic liquid and additive were revealed by NMR and IR analysis. It was found that the formation of hydrogen bonds between 3-aminopropanol and [EMIM]Ac changed the interactions between ionic liquids and biomass, resulting in improved dissolution efficiency and inhibition of cellulose degradation. Optimization investigation showed that when using the 3-aminopropanol/[EMIM]Ac composite system as the solvent and pine as the raw biomass, the cellulose content in the recovered cellulose-rich material was increased from 33.3% (for the raw pine) to 66.9%. Correspondingly, the regenerated cellulose spinning in the composite system exhibited improved mechanical properties, with the elongation at break reaching 15.6% and the tensile fracture strength of 184.1 N per tex (in comparison with 9.6% for elongation at break and 99.7 N per tex for tensile fracture strength for the sample obtained in neat [EMIM]Ac).
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ADVANCED FUNCTIONAL MATERIALS
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2023
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IF 19
Ting Zhang,Yuting Li,Xiaoming Lv,Shen Jiang,Shuang Jiang,Zhongqi Sun,Mingxu Zhang,Yang Li
The detection of biomolecules utilizing surface-enhanced Raman spectroscopy (SERS) is highly trending in research, but the unlabeled and sensitive detection of saccharides in body fluids is challenging. Here, a novel SERS substrate (AgNS 600 ) is fabricated by physical scratching of smooth silver sheets, which successfully achieves enhanced SERS fingerprinting by capturing glucose molecules through nanoscale grooves on the surface of the substrate to form multi-dimensional “hot spot” regions. The platform proves to be rapid, reliable, and reproducible, with a lower detection limit of 0.5 amol L −1 in deionized water. Further detection of multiple monosaccharides, polysaccharides, and mix samples, which are distinguished with machine learning and heat map, demonstrates the versatility and specificity of the platform. Importantly, real samples are explored and the results show that the platform is able to detect and recognize SERS signals of glucose in the blood, urine, tears, and perspiration, and non-destructive predict diabetes by analyzing the signal intensity of different characteristic peaks. In this work, it is the first non-chemical, un labeled determination of glucose with high sensitivity via SERS, which provides a potential strategy for advancing the clinical development of SERS technology and the early diagnosis of glucose metabolic diseases.
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JOURNAL OF MEMBRANE SCIENCE
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2021
·
IF 8.742
Zhengyang Gu,Ping Li,Xuerui Gao,Yuan Qin,Yishuai Pan,Youbing Zhu,Shuili Yu,Qing Xia,Yanling Liu,Dongsheng Zhao,Guicai Liu
Hydrophilic and porous nanofillers have shown great benefits in the attainment of high-performance nanofiltration (NF) membranes. In this work, a novel nanoporous covalent organic framework (NCOF) was facilely synthesized under mild conditions and deposited onto a polyethersulfone substrate to induce formations of crumpled polyamide layers via interfacial polymerization. With the incorporation of NCOFs, the surface of the COF-based thin-film nanocomposite (CTN) membranes gradually transformed from tent-like to mesh morphology, which significantly enhanced the water permeance owing to the increased effective membrane filtration area and additional water transport channels provided by NCOFs. Besides, the favorable compatibility between NCOFs and the polyamide layer endowed the CTN membranes with high rejection capacities to a variety of solutes. The optimal membrane with the NCOF deposition density of 19.27 μg cm −2 , CTN9 membrane, showed nearly twice higher water permeance of 15.5 L m −2 h −1 bar −1 while maintaining high rejections of Na 2 SO 4 and MgCl 2 (98.9% and 94.2%, respectively). In addition, the CTN membranes exhibited a satisfying stability during the long-term filtration of saline solutions as well as an improved antifouling ability to the model foulants of bovine serum albumin. The superior NF performance rendered the NCOF functionalized membranes highly promising for water treatment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Ximeng Lin,Wenxia Li,Hywel Yuen,Michael Yuen,Qiang Peng
Agaricus bitorquis (QuéL.) Sacc. Chaidam is a valuable edible fungus in Qinghai-Tibet plateau and ABSP is a novel intracellular polysaccharide from its mycelia. GC and NMR analysis determined ABSP is galactoglucomannan-like polysaccharide that may have immunomodulatory effect. This study used RAW264.7 as model cell to determine immunomodulatory effect of ABSP. After ABSP treatment, viability and phagocytic ability promoted, and NO, ROS, TNF-α levels also raised which proved ABSP had immune regulation to RAW264.7. WB and qRT-PCR determined the key proteins and genes expression of TLR4, MyD88, TRAF-6 and NF-κB significantly increased while protein and gene expression of TRAM had no significant increase. Also, TNF-α level extremely decreased by adding inhibitors of TLR4 and MyD88 which confirmed ABSP could immunologically regulate RAW264.7 byTLR4-MyD88 dependent pathway. This study would provide theoretical basis for further study on ABSP and be helpful for development of beneficial functionally foods and exploitation of this resource.
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高影响因子
MOLECULES
·
2021
·Vol. 26
·期 8
·2208
·
IF 4.412
Muhammad Sajid,Muhammad Rizwan Dilshad,Muhammad Saif Ur Rehman,Dehua Liu,Xuebing Zhao
Furfural is one of the most promising precursor chemicals with an extended range of downstream derivatives. In this work, conversion of xylose to produce furfural was performed by employingp-toluenesulfonic acid (pTSA) as a catalyst in DMSO medium at moderate temperature and atmospheric pressure. The production process was optimized based on kinetic modeling of xylose conversion to furfural alongwith simultaneous formation of humin from xylose and furfural. The synergetic effects of organic acids and Lewis acids were investigated. Results showed that the catalystpTSA-CrCl3·6H2O was a promising combined catalyst due to the high furfural yield (53.10%) at a moderate temperature of 120 °C. Observed kinetic modeling illustrated that the condensation of furfural in the DMSO solvent medium actually could be neglected. The established model was found to be satisfactory and could be well applied for process simulation and optimization with adequate accuracy. The estimated values of activation energies for xylose dehydration, condensation of xylose, and furfural to humin were 81.80, 66.50, and 93.02 kJ/mol, respectively.Keywords:catalysis;chlorides;furfural;kinetics;lewis acid;p-toluenesulfonic acid;xylose
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CHINESE JOURNAL OF CHEMISTRY
·
2021
·Vol. 39
·期 9
·2467-2476
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IF 6
Liangqing Zhang,Jiarong Qiu,Xing Tang,Yong Sun,Xianhai Zeng,Lu Lin
Main observation and conclusion A series of catalysts were prepared for sugar alcohols production to overcome the deficiencies of the previous reported catalysts, such as low yield of sugar alcohols, single function, instability, and controversial role of active sites. The role of each metal and their synergistic-cooperation was discussed in detail with a combination of conditional experiments and characterizations. The results indicated that bifunctional Ni 6.66 Fe 1 Al 1.55 catalyst has unique structure with superparamagnetism and excellent activity. The (111) and (200) planes of metallic Ni are the hydrogenation active phases and preferentially exposed on Ni-Al-O x spinel. The desired arabitol or mannitol was obtained by tuning the ratio of Brønsted and Lewis acid sites. The recycling tests indicated that the unique structure of the prepared Ni-based catalyst can suppress leaching and poisoning, which has high textural stability and activity.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2021
·
IF 19.503
Yancong Li,Jiliang Ma,Dongnv Jin,Gaojie Jiao,Xiaopan Yang,Kangning Liu,Jinghui Zhou,Runcang Sun
Much attention has been focused on the photocatalytic-reforming of biomass to produce high-value chemicals due to their unique merits. In this study, we report a freezing-thawing strategy for the preparation of an alkaline CuO-chitosan hybrid hydrogel (CuO@CS-H). The synergism between CuO and alkaline chitosan hydrogel led to a highly efficient photocatalytic-reforming of xylose to lactic acid under a weak alkaline system, and the highest lactic acid yield is 81.6 %. The retained lactic acid yield was about 91.2 % after being used nine times. The reaction system is universal for the production of lactic acid from biomass-derived pentoses and hexoses, and O 2 − plays a major role in the synthesis of lactic acid. Industrial production of lactic acid via the as-proposed system was shown to be feasible according to a two-thousand-fold scale-up experiment. This work initiates a novel strategy of photocatalytic synthesis of lactic acid from biomass-based monosaccharides.
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JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2021
·
IF 3.935
Yilin Zhang,Mengqi Wu,Jingrui Xi,Chen Pan,Zhizhen Xu,Wei Xia,Wenqing Zhang
In this study, the multiple fingerprints, which were integrated with HPGFC-ELSD (high performance gel filtration chromatography - evaporative light scattering detector) fingerprint, PMP-HPLC-DAD (1-phenyl-3-methyl-5-pyrazolone-high performance liquid chromatography - diode array detector) fingerprint of complete acid hydrolysates and HILIC-HPLC-ELSD (hydrophilic interaction - high performance liquid chromatography - evaporative light scattering detector) fingerprint of enzyme hydrolysates, were established to evaluate the quality of polysaccharides from Poria cocos (PCPs). The similarity evaluation showed that 16 batches of PCPs from different origins had high similarity in structural characteristics based on the multiple fingerprints. The chromatographic data of multiple fingerprints of PCPs were fused, processed and analyzed by chemometric methods including HCA (hierarchical cluster analysis), PCA (principal component analysis) and PLS-DA (partial least squares discriminant analysis). The 16 batches of PCPs were divided into 3 categories in PCA, indicating a certain relationship between the structural characteristics and the origins. PLS-DA analysis indicated that Man, Glc, Gal, Fuc, the components with m/z of 2.22 × 10 4 ∼1.53 × 10 4 Da and 3.46 × 10 3 ∼2.69 × 10 3 Da, oligosaccharides with DPs of 6 and 7, respectively, could be regarded as potential chemical markers for the classification of PCPs from different origins. According to the multiple fingerprints and chemometric analysis, the two commercial samples were proved to be adulterants.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Huashan Gao,Linlin Ding,Rui Liu,Xinhua Zheng,Xichao Xia,Fuan Wang,Jinxu Qi,Weishuang Tong,Yuanhao Qiu
Anoectochilus roxburghii is a traditional herb in China that can be potentially used to treat diabetes. A novel polysaccharide ARLP-W was isolated from Anoectochilus roxburghii by chromatography on DEAE-52 cellulose. Chemical analysis indicated that ARLP-W (8.1 × 10 4 Da) was mainly composed of mannose and glucose. The main linkages of glycosidic bonds of ARLP-W were β-1, 4-Manp and α-1, 4-Glcp. The terminal Glcp was connected to Manp-via O-3. RT-qPCR and western blotting analysis showed that ARLP-W caused a significant reduction in the levels of the key gluconeogenesis enzymes phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6-phosphatase (G6Pase) in the liver. The results of the insulin resistance tests indicated that ARLP-W increased glucose absorption. These results indicate that ARLP-W has a good therapeutic effect on type 2 diabetes and can assist with further development and application treatment of diabetes.
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