INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2014
·
IF 2.235
Jiahe Yan,Yucui Hou,Shuhang Ren,Muge Niu,Weize Wu
Because of its controllable and recoverable properties, the H2O–SO2 system was studied as a reaction medium to hydrolyze corn cob to produce chemicals by a two-step treatment. The results indicated that, after the first-step treatment of corn cob, 27.0% xylose, 3.3% glucose, and 3.2% arabinose could be obtained at 140 °C for 1.5 h with 0.024 g/cm3 SO2; after the second-step treatment, 8.6% glucose could be obtained at 190 °C for 0.5 h with 0.048 g/cm3 SO2. The total yield of products by the two-step treatment of corn cob was 48.4%, which was greater than that obtained by the one-step treatment, 32.4% at 190 °C for 0.5 h with 0.048 g/cm3 SO2. Furthermore, the degradation products obtained from the two-step treatment were less than those obtained from the one-step treatment. More importantly, after the reaction, SO2 could be swept by stream stripping and reused.
研究领域:
生物化学
·
可再生资源利用
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2017
·
IF 3.359
Kaifei Chen,Gang Luo,Zhongfang Lei,Zhenya Zhang,Shicheng Zhang,Jianmin Chen
Recovery of monosaccharides from hydrolysates of biomass wastes can bring environmental and economic benefits. This study aimed to explore the feasibility to separate major monosaccharides from hydrolysates of lignocellulosic biomass, i.e. glucose, xylose and arabinose by using column chromatography process. Cation exchange resin Amberlite IR120 and Amberlite IRP69 in Na + and Ca 2+ forms were chosen as adsorbents. The adsorption behaviors were firstly inspected by adsorption equilibrium and mass transfer coefficients. Then the optimal chromatography conditions were determined by using synthetic solution. Finally, hydrothermal liquefaction hydrolysate of pine branches was separated. The results showed that cation resin Amberlite IRP69 (Ca 2+ ) had high adsorption selectivity for arabinose which could be recovered from synthetic solution with purity of 92%. The highest purity of 88% was achieved for xylose when using this resin to separate pretreated hydrolysate. This work suggests that cation exchange resin could be further developed for effective separation of monosaccharides mixture.
研究领域:
生物化学
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环境科学
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DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Junjun Zhu,Han Zhang,Ningxin Jiao,Yuxin Xiao,Dawei Shi,Yong Xu
This study proposed a clean sequential process, including autohydrolysis, the hydrolysis of endoxylanase, and adsorption with activated carbon (AC), that assisted refining poplar ( Populus spp.) into low degree of polymerization (DP) xylooligosaccharides (XOS). First, autohydrolysis was conducted at 170 °C for 50 min. The autohydrolysate was then hydrolyzed with 1.0 International Unit (IU) per mL of endoxylanase to increase the concentration of low DP XOS (X2-X4) from 2.98 to 6.42 g/L. The recovery of XOS from the XOS rich enzymatic hydrolysis liquor reached 96.5% using 10% AC with a gradient elution of ethanol, and the highest purity of XOS was 98.2% from the 40% ethanol eluent. Finally, the separated spent liquor (SL) obtained after AC adsorption was assayed for bioactivity on rice ( Oryza sativa L.). Spent liquor revealed a positive dose-dependent bioactivity toward rice growth, which promoted seed germination, plant height, root length and fresh weight. These effects were attributed to an appropriate amount of acetic acid and XOS, which could be used as plant biostimulants.
研究领域:
生物化学
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植物学
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产品关联
DOI
高影响因子
ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Dawei Dong,Yafei Xiao,Minghua Zhang,Peixin Gong,Yingqing Ye,Shuhua Peng,Ke Wang,Minmin Fan
Polysaccharide is a kind of biological macromolecule with a complex structure, which plays an irreplaceable role in the organism. Due to the complex purification process of natural polysaccharide, chemical synthesis is an important means to obtain it. In this work, a green and efficient solid-phase polymerization method was used to synthesize glucan polysaccharide from unprotected glucose in one step under mild solid-phase conditions, using Hβ zeolites as acidic catalyst. The final polysaccharide product was characterized by high-performance liquid chromatography (HPLC) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) with a conversion of 35.0% and a maximum degree of polymerization of 26. More importantly, it was speculated that the glycosylation of unprotected glucose catalyzed by Hβ zeolites conforms to the Fischer glycosylation mechanism. In addition, the results of methylation analysis and one-dimensional, two-dimensional nuclear magnetic resonance (1D, 2D NMR) showed that the resulting polysaccharides were mainly linear, containing 10 different glycosidic linkages, and (1,6-Glcp), (1-Glcp), and (1,4-Glcp) were the most abundant linkage types among them.
研究领域:
有机化学
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生物化学
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DOI
高影响因子
Biochimica et Biophysica Acta-Gene Regulatory Mechanisms
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2018
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IF 5.179
Quan Gan,Xinwei Zhang,Daobo Zhang,Liang Shi,Yue Zhou,Tongtong Sun,Song Jiang,Junshan Gao,Yan Meng
Sucrose is the most commonly transported sugar in plants and is easily assimilated by insects to fulfill the requirement of physiological metabolism. BmSuc1 is a novel animal β-fructofuranosidase (β-FFase, EC 3.2.1.26) - encoding gene that was firstly cloned and identified in silkworm , Bombyx mori . BmSUC1 was presumed to play an important role in the silkworm-mulberry enzymatic adaptation system by effectively hydrolyzing sucrose absorbed from mulberry leaves. However, this has not been proved with direct evidence thus far. In this study, we investigated sucrose hydrolysis activity in the larval midgut of B. mori by inhibition tests and found that sucrase activity mainly stemmed from β-FFase, not α-glucosidase. Next, we performed shRNA-mediated transgenic RNAi to analyze the growth characteristics of silkworm larvae and variations in glycometabolism in vivo in transgenic silkworms. The results showed that in the RNAi- BmSuc1 transgenic line, larval development was delayed, and their body size was markedly reduced. Finally, the activity of several disaccharidases alone in the midgut and the sugar distribution, total sugar and glycogen in the midgut, hemolymph and fat body were then determined and compared. Our results demonstrated that silencing BmSuc1 significantly reduced glucose and apparently activated maltase and trehalase in the midgut. Together with a clear decrease in both glycogen and trehalose in the fat body, we conclude that BmSUC1 acts as an essential sucrase by directly modulating the degree of sucrose hydrolysis in the silkworm larval midgut, and insufficient sugar storage in the fat body may be responsible for larval malnutrition and abnormal petite phenotypes.
研究领域:
昆虫学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Changyan Dong,Yi Cheng,Meihui Zhang,Mianhua Chen,Zhongli Yan,Sumei Zhou,BenxuYang,Qingbin Guo,Changlu Wang,Shufen Wu
In this study, four Monascus pigments (ankaflavin, AK; monascin MS; rubropunctatin, O 1 ; monascorubrin, O 2 ) were proved to exhibit considerable anti-glycation properties in bovine serum albumin (BSA)-fructose model. AK (40.62 %) and MS (48.38 %) were found to exert lower inhibitory effects on the formation of fluorescent advanced glycation end products (AGEs) than aminoguanidine (59.4 %), while O 1 (90.64 %) and O 2 (93.82 %) displayed much stronger abilities. AK and MS could trap methylglyoxal (MGO) with maximum capture rates of 85.67 % and 84.90 %, respectively, and only mono-MGO adducts of them were detected. LC-Orbitrap MS/MS analysis revealed that four pigments significantly altered the type and reduced the number of the glycated sites and they all covalently bound to BSA, with O 1 and O 2 possessing high reactivity. Altogether, AK and MS suppressed fluorescent AGEs formation mainly via trapping MGO and covalently interacting with BSA, and blocking free amino groups was the dominant mechanism for O 1 and O 2 . These findings presented new insights into Monascus pigments as dietary supplement for inhibiting protein glycation.
研究领域:
生物化学
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食品科学
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED TOXICOLOGY
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2020
·Vol. 40
·期 9
·1284-1296
·
IF 2.997
Zhangjian Chen,Shuo Han,Pai Zheng,Shupei Zhou,Guang Jia
Titanium dioxide nanoparticles (TiO 2 NPs) were usually consumed with a high content of sugar, and children were identified as having the highest exposures due to sweet food preferences. Research on the combined effect of ingested TiO 2 NPs and glucose has great significance, particularly in young people. We examined young Sprague-Dawley rats administered TiO 2 NPs (0, 2, 10 and 50 mg/kg) orally with and without glucose (1.8 g/kg) for 90 days. Blood glucose homeostasis was assessed by monitoring blood glucose and detecting glycoproteins. Glucose tolerance was also evaluated by the oral glucose tolerance test. The levels of blood glucose-related hormones such as insulin, C-peptide and glucagon were measured. We found that subchronic co-exposure of TiO 2 NPs and glucose caused slight imbalance of blood glucose homeostasis in vivo. Mild and temporary hypoglycemia, impaired glucose tolerance and changes of glucose-regulating hormones were shown in the exposure groups. The combined effect of TiO 2 NPs and glucose was more apparent than that of TiO 2 NPs alone, which may be due to the effects of excess glucose and the interactions between TiO 2 NPs and glucose. The antagonistic effect of TiO 2 NPs with glucose did exist in the level of glycosylated hemoglobin in female rats. Gender differences were apparent in these effects induced by TiO 2 NPs and glucose. Female rats seemed to be more susceptible for blood glucose disorders. Co-exposure of TiO 2 NPs and excessive glucose could induce gender-dependent imbalance of blood glucose homeostasis in rats. It may be the reason that these consumers face greater health risks glycosylated hemoglobin.
研究领域:
毒理学
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内分泌学
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营养科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2018
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IF 4.946
Yang Chen,Zong-cai Tu,Hui Wang,Guang-xian Liu,Zi-wei Liao,Lu Zhang
In general, the reducing sugars were extensively utilized as additives to prevent denaturation and inactivation during the freeze-drying process. However, different additives have unequal protective effects on the protein conformation. To evaluate the mechanism of protection the protein structure using different additives in the freeze-drying process, the glycated sites and degree of substitution per peptide (DSP) of each site were investigated by LC-Orbitrap MS. We found that the ovalbumin that was supplemented with ribose and then lyophilized (R-oval) have the highest extent of glycation modification. K227 was the most reactive glycated site in R-Oval, with a DSP of 0.83. The ovalbumin that was supplemented with lactose and then lyophilized (L-Oval) have the lowest degree of glycation, and K227 did not undergo the glycation reaction. It was hypothesized that the order impact of different additives on protection of the protein conformation were lactose > galactose > ribose during the freeze-drying process.
研究领域:
分析化学
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蛋白质化学
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药学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2018
·
IF 3.909
Lu Zhao,Youzhi Zhou,Song Qin,Panpan Qin,Jianlin Chu,Bingfang He
β-Galactosidases can catalyze the hydrolysis of lactose and the synthesis of galacto-oligosaccharides (GOS) from lactose. The catalytic activity of β-galactosidases is usually inhibited by galactose and glucose, which hampers the complete hydrolysis of lactose in food products. In this report, a β-galactosidase (denoted as BMG) from Bacillus megaterium YZ08 without the inhibition by galactose and glucose is the first to our knowledge reported. Efficient secretory expression of BMG was successfully achieved in Bacillus subtilis WB800. With the increasing of galactose and glucose, the activity of BMG dramatically enhanced. With addition of 0.6 M galactose or 1 M glucose, the initial activity to o NPG hydrolysis was increased by 2.1-fold and 4.3-fold, respectively. Moreover, the synthetic rate of galacto-oligosaccharides was enhanced by 1.1-fold and 1.9-fold with the addition of 0.7 M galactose or glucose, respectively. The GOS (278 g/L) from 600 g/L lactose by BMG were efficiently produced within 12 h, and the substrate lactose was completely used up. These results suggest that BMG shows a potential application in the food industry.
研究领域:
生物化学
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生物技术
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 4
·1974-1980
·
IF 4.125
Hengtao Tang,Zhi Chen,Yu Shao,Xin Ju,Liangzhi Li
BACKGROUND The fermentation valorization of two main lignocellulosic monosaccharides, glucose and xylose, is extensively developed; however, it is restricted by limited yield and process complexity. An in vitro enzymatic cascade reaction can be an alternative approach. RESULTS In this study, a three-stage, five-enzyme cascade was developed to convert pretreated biomass to valuable chemicals. First, a ribose-5-phosphate isomerase B mutant isomerized xylose to d -xylulose with high substrate specificity, and a d -arabinose dehydrogenase continued to reduce d -xylulose to d -arabitol. Simultaneously, glucose was utilized for the coenzyme regeneration catalyzed by a glucose dehydrogenase, generating useful gluconic acid and achieving 73% of total conversion rate after 36 h. Then, six kinds of pretreated biomass lignocellulose were hydrolyzed by cellulase and hemicellulase, and corn cob was identified as the initial substrate for providing the highest monosaccharide content. A 65% conversion rate of the lignocellulosic xylose was obtained after 24 h. CONCLUSIONS This study presents a proof of concept to convert main lignocellulosic monosaccharides systematically by an enzymatic cascade at stoichiometric ratio. © 2022 Society of Chemical Industry.
研究领域:
生物技术
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生物化工
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酶学
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2025
·
IF 12.5
Xinxiong Wang,Haojie Qiu,Zhongrun Xiang,Longxiang Tang,Huiqing Wang,Lei Xue,Xuefeng Yang
Due to the hyperglycemic environment, diabetic wounds are highly susceptible to bacterial infections, facilitating their progression to chronic, non-healing wounds. Consequently, there is an urgent need to develop wound dressings capable of reducing wound glucose levels and exerting strong antibacterial activities. In this study, a ZIF-8/Au-GOx (ZAG) nanozyme was grown in situ on fluffy chitin (FC) powder by integrating zeolitic imidazolate framework-8 (ZIF-8), glucose oxidase (GOx), and Au nanoparticles, leading to the development of a multifunctional wound dressing (FC/ZAG) for diabetic wound therapy. Benefiting from the cascade catalytic effect of the loaded ZAG nanozyme, FC/ZAG demonstrated a remarkable hypoglycemic activity and potent antibacterial properties. Additionally, owing to the biocompatibility of FC and the sustained release of Zn 2+ , the FC/ZAG powder exhibited a superior safety profile. In experiments in a mouse model of diabetic wound healing, FC/ZAG exerted significant anti-inflammatory and pro-angiogenic effects, accelerating the healing process of diabetic wounds. These findings highlight the high potential of FC/ZAG as a promising candidate for the development of advanced diabetic wound dressings.
研究领域:
生物材料学
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纳米医学
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创伤修复与再生医学
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糖尿病并发症
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感染控制
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酶催化
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药物递送
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产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2017
·
IF 1.837
Zixing Dong,Xiaoming Hao,Daman S. Pokhrel,Xiaoling Chen,Xiaoguang Liu,Nokuthula P. Mchunu,Kugenthiren Permaul,Suren Singh,Dandan Niu,Zhengxiang Wang
High-glucose syrup has wide applications in the feed and fermentation industries. However, during its regular production process, because of the slow hydrolysis of α-1,6-glucosidic linkages in maltodextrins by glucoamylase, yields of glucose higher than 96% are rarely achieved. To find a suitable enzyme for the saccharification process, two novel genes encoding oligo-1,6-glucosidases (OGL, EC 3.2.1.10) from Bacillus mycoides ( bmogl ) and Thermomyces lanuginosus ( tlogl ) were successfully cloned and overexpressed in Pichia pastoris GS115. At the shake-flask fermentation level, the OGL activities of the two recombinants GS115 (pPIC9K- bmogl ) and GS115 (pPIC9K- tlogl ) were 994 and 1219 U/mL, respectively; and mature enzymes around 66–68 kDa were purified for characterization. Recombinant enzyme TlOGL exhibited higher thermostability than ever reported for OGLs, whereas BmOGL was stable under acidic pH, ranging from 4.0 to 8.0. Among the substrates tested, these two recombinant enzymes efficiently hydrolyzed isomaltose and isomaltotriose, but had no activity against maltose and maltotriose. Besides these characteristics, nearly complete hydrolysis of isomaltotriose and 90% of isomaltooligosaccharides (IMOs) into glucose was also observed for them, which makes them good candidates for subsequent use in improving the yield of glucose from starch. To the best of our knowledge, this is the first report on the expression and characterization of OGLs from B. mycoides and T. lanuginosus .
研究领域:
生物化学
·
分子生物学
·
工业酶学
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DOI
高影响因子
危险品化学品经营许可证（带存储）