Journal of Translational Medicine
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2022
·Vol. 20
·期 1
·1-10
·
IF 8.44
Huang Kun,Wang Dan-Dan,Hu Wen-Bao,Zeng Wei-Qian,Xu Xia,Li Qiu-Xiang,Bi Fang-Fang,Yang Huan,Qiu Jian
Background Myotonic dystrophy type 1 (DM1), one of the most common forms of adult-onset muscular dystrophy, is caused by abnormally expanded CTG repeats in the 3′ untranslated region of the DMPK gene. The CUG repeats transcribed from the expanded CTG repeats sequestrate a splicing factor, MBNL1, causing the clinical symptoms in DM1. Nowadays, only symptomatic treatments are available for DM1, and no rational therapy is available. Recently, upregulation of MBNL1 expression has been found to be one of the promising therapies for DM1.Methods All experiments were conducted in the C2C12 myoblasts and HSALR mice, a DM1 mouse model. Real-time PCR and western blot were used to detect the mRNA and protein level, respectively. The rotarod exercise, grip strength and hanging time were used to evaluate the muscle strength of mice.Results In this study, we demonstrated that calcitriol, an active form of vitamin D3, increased MBNL1 in C2C12 mouse myoblasts as well as in HSALR mice model for DM1. In HSALR mice model, calcitriol improved muscle strength, and corrected aberrant splicing in skeletal muscle. Besides, calcitriol reduced the number of central nuclei, and improved muscle histopathology in HSALR mice. In addition, we identified that calcitriol upregulated MBNL1 expression via activating the promoter of Mbnl1 in C2C12 myogenic cells.Conclusion Our study suggests that calcitriol is a potential pharmacological strategy for DM1 that enhances MBNL1 expression.
研究领域:
分子生物学
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神经病学
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药理学
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肌肉骨骼疾病
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2023
·
IF 5.6
Yang Yang,Cuiting Lin,Qiang Zheng,Leqi Zhang,Yongmei Li,Qinghua Huang,Ting Wu,Zean Zhao,Lu Li,Jian Luo,Yanqing Jiang,Qun Zhang,Xing Wang,Chenglai Xia,Jianxin Pang
Hyperuricemia (HUA) is associated with left ventricular remodeling (LVR) and thereby causes the initiation and development of a large number of cardiovascular diseases. LVR is typically accompanied by cardiomyocyte energy metabolic disorder. The energy supply of cardiomyocytes is provided by glucose and fatty acid (FA) metabolism. Currently, the effect of HUA on cardiomyocytic FA metabolism is unclear. In this study, we demonstrate that UA-induced cardiomyocyte injury is associated with cytoplasmic lipid deposition, which can be ameliorated by the FA metabolism-promoting drug L-carnitine (LC). UA suppresses carnitine palmitoyl transferase 1B (CPT1B), thereby inhibiting FA transport into the mitochondrial inner matrix for elimination. LC intervention can ameliorate HUA-associated left ventricular anterior wall thickening in mice. This study showed that FA transport dysfunction plays is a critical mechanism in both cardiomyocytic injury and HUA-associated LVR and promoting cytoplasmic FA transportation through pharmacological treatment by LC is a valid strategy to attenuate HUA-associated LVR.
研究领域:
心脏病学
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代谢紊乱
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药理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2023
·
IF 5.4
Wei Jia,Xiwen He,Wenhui Jin,Jinping Gu,Siyu Yu,Jianlin He,Zhiwei Yi,Bing Cai,Huiyuan Gao,Longhe Yang
Ethnopharmacological relevance Ramulus Cinnamomi , the dried twig of Cinnamomum cassia (L.) J.Presl., is a traditional Chinese medicine (TCM) with anti-inflammatory effects. The medicinal functions of Ramulus Cinnamomi essential oil (RCEO) have been confirmed, although the potential mechanisms by which RCEO exerts its anti-inflammatory effects have not been fully elucidated. Aim of the study To investigate whether N -acylethanolamine acid amidase (NAAA) mediates the anti-inflammatory effects of RCEO. Materials and methods RCEO was extracted by steam distillation of Ramulus Cinnamomi , and NAAA activity was detected using HEK293 cells overexpressing NAAA. N -Palmitoylethanolamide (PEA) and N -oleoylethanolamide (OEA), both of which are NAAA endogenous substrates, were detected by liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The anti-inflammatory effects of RCEO were analyzed in lipopolysaccharide (LPS)-stimulated RAW264.7 cells, and the cell viability was measured with a Cell Counting Kit-8 (CCK-8) kit. The nitric oxide (NO) in the cell supernatant was measured using the Griess method. The level of tumor necrosis factor-α (TNF-α) in the RAW264.7 cell supernatant was determined using an enzyme-linked immunosorbent assay (ELISA) kit. The chemical composition of RCEO was assessed by gas chromatography-mass spectroscopy (GC-MS). The molecular docking study for (E)- cinnamaldehyde and NAAA was performed by using Discovery Studio 2019 software (DS2019). Results We established a cell model for evaluating NAAA activity, and we found that RCEO inhibited the NAAA activity with an IC 50 of 5.64 ± 0.62 μg/mL. RCEO significantly elevated PEA and OEA levels in NAAA-overexpressing HEK293 cells, suggesting that RCEO might prevent the degradation of cellular PEA and OEA by inhibiting the NAAA activity in NAAA-overexpressing HEK293 cells. In addition, RCEO also decreased NO and TNF-α cytokines in lipopolysaccharide (LPS)-stimulated macrophages. Interestingly, the GC-MS assay revealed that more than 93 components were identified in RCEO, of which (E)- cinnamaldehyde accounted for 64.88%. Further experiments showed that (E)- cinnamaldehyde and O -methoxycinnamaldehyde inhibited NAAA activity with an IC 50 of 3.21 ± 0.03 and 9.62 ± 0.30 μg/mL, respectively, which may represent key components of RCEO that inhibit NAAA activity. Meanwhile, docking assays revealed that (E)- cinnamaldehyde occupies the catalytic cavity of NAAA and engages in a hydrogen bond interaction with the TRP181 and hydrophobic-related interactions with LEU152 of human NAAA. Conclusions RCEO showed anti-inflammatory effects by inhibiting NAAA activity and elevating cellular PEA and OEA levels in NAAA-overexpressing HEK293 cells. (E) -cinnamaldehyde and O- methoxycinnamaldehyde, two components in RCEO, were identified as the main contributors of the anti-inflammatory effects of RCEO by modulating cellular PEA levels through NAAA inhibition.
研究领域:
药理学
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免疫学
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细胞生物学
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生物化学
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中医
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2016
·Vol. 41
·期 3
·e12915
·
IF 0.894
Meng Xu,Wei Liu,Yawei Zhang,Zengqi Peng,Xiuyun Guo,Fulong Wang
Orthophosphate (Pi), tetrasodium pyrophosphate (PP) and sodium tripolyphosphate (TPP) in the polyphosphatases and longissimus thoracis from beef were detected by ion chromatography. Under pure solution models, tripolyphosphatase (TPPase) interacted with pyrophosphatase (PPase). Single TPP hydrolysis was slower than the polyphosphate blend (the ratio of PP, TPP and sodium hexametaphosphate was 3:4:3). Within 4 h, 0.0896 g/L of TPP remained in the TPP + PPase + TPPase system, while it was hydrolyzed completely in the polyphosphate blend + PPase + TPPase system, which was different under minced meat models. The PP, TPP and polyphosphate blend were hydrolyzed slowly under minced meat models. The acceleration or deceleration of the entire polyphosphates hydrolysis process could be due to the ratio of PPase to TPPase and their activities. These results could be useful in the development of an optimal ratio of polyphosphate mixture for the meat and meat products in food industry. Practical Applications Polyphosphates play an important role in meat processing. They can increase the water holding capacity of muscle proteins, reduce cooking loss, improve meat tenderness and gel properties, and affect the ability to emulsify. It is commonly used commercially as a blend of various polyphosphates (including tetrasodium pyrophosphate, sodium tripolyphosphate and sodium hexametaphosphate) in meat processing. There have been few reports on the mechanism through which polyphosphate blend improve the functionalities of muscle proteins. In this work, the dynamic changes in the hydrolysis of tetrasodium pyrophosphate, sodium tripolyphosphate and polyphosphate blend under purified polyphosphatases and longissimus thoracis from beef using free reagent ion chromatography was observed. This could be helpful in understanding the mechanism of polyphosphate hydrolysis and in developing an optimal ratio of polyphosphates blends for meat and meat products in industry.
研究领域:
食品科学
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生物化学
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肉类技术
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产品关联
DOI
高影响因子
FOOD HYDROCOLLOIDS
·
2024
·
IF 11
Yuxin Cheng,Jiaxin Xu,Rongfan Zhang,Jianguo Lin,Meiling Zhou,Xianmin Qin,Keshan Wang,Ying Zhou,Qiujin Zhu,Yongguo Jin,Yuanyuan Liu
The design and development of a food preservation coating material with rapid curing and stable performance to shorten the coating process time and reduce quality changes caused by temperature fluctuations in cold chain is highly desirable in fresh meat preservation applications. In this study, a series of multi-cross-linked edible hydrogels were designed based on gelatin and k-carrageenan through the self-assembly triple-helix of gelatin and the potassium ion-mediated k-carrageenan double-helix structure. The tannic acid with phenolic hydroxyl groups was employed to enable the dynamic bond cross-linking between two polymers, endowing the composite hydrogel with rapid curing ability at low-temperature and a uniform molecular network structure. The hydrogel with a concentration of gelatin was 16% (GCBG-16) achieved the optimal combination properties, including 73.41% of elongation at break, 71.59 kJ/m 2 of toughness, 27.73% of swelling ratio, and 50.73% of DPPH· scavenging rate. The hydrogels could be rapidly washed within 30 min at 40 °C by water, as well as possessing a certain self-healing and anti-freezing capabilities. The hydrogel GCBG-16 had a great capacity for protecting meat with a E value of 50.4 and 17.53 N of shear force even placed in a 37 °C environment, which showed no significant difference (p > 0.05) compared to the initial value. Furthermore, the fresh meats with coating showed delayed central temperature increase speed after leaving from the cold chain and placed in a 37 °C environment. The research results demonstrated that the proposed hydrogel has great potential in preserving fresh meat, which can serve as a matrix material combined with other active additives for extending the shelf life of meat products.
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
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2022
·
IF 10.383
Lei Liu,Cheng Wang,Ze Wu,Youqiang Xing
The main challenge in manufacturing an ionic actuator of large bending displacement and great response sensitivity is to design a flexible electrode with great electrochemical characteristics and conductivity. This research reports the MXene-PEDOT:PSS/AgNWs (MPA) electrode with a three-dimensional (3D) network structure formed by a hybrid method of the one-dimensional (1D) silver nanowires (AgNWs) and the two-dimensional (2D) Ti3C2Tx MXene. Here, a soft actuator based on the ionic cross-linked hybrid electrode was designed. The results show that the MPA electrode-based soft actuator achieves a large bending strain (0.48%, ±0.5 V sine voltage), wide frequency (0.1–10 Hz), 5 h durability (91.9% retention), fast response time (≈5 s), great power density (7.53 kW m–3), and great energy density (18.83 kJ m–3). These excellent performances contribute to the 3D structure of electrodes formed by MXene and AgNWs creating an unhindered ion channel, which facilitates short diffusion and rapid injection of ions and provides higher capacitance and mechanical integrity. This 3D network layered structure hybrid electrode provides an opportunity for the development of ultralow-voltage-drivable artificial muscles.
研究领域:
材料科学
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生物医学工程
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DOI
PubMed
高影响因子
WOOD SCIENCE AND TECHNOLOGY
·
2012
·Vol. 47
·期 3
·601-613
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IF 1.727
Si Hongyan,Li Bin,Wang Ting,Lin Li,Xu Zuwei
Poplar wood flour, a low-cost and abundantly available material, was modified by grafting β -cyclodextrin ( β -CD) in the presence of citric acid or 1,2,3,4-Butanetetracarboxylic acid, aiming to expand the application of wood flour. The products were characterized by Fourier transform infrared spectroscopy and the technique of phenolphthalein probe. The grafting conditions of β -CD onto wood flour including curing temperature, β -CD concentration in impregnation solution and catalyst were also evaluated by the technique of phenolphthalein probe. Besides, eugenol (EG), an antibacterial fragrant guest molecule, was loaded on the modified wood flour by lyophilization, and its release characteristics were investigated by Thermo-gravimetric. The results revealed that the modified wood flour material had a thermo-protective effect on the volatilization of EG and the encapsulated EG content was reached to 1.31 %, which could demonstrate a potential application in the finishing and packing areas.
研究领域:
材料科学
·
化学工程
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产品关联
DOI
高影响因子
Processes
·
2020
·Vol. 8
·期 7
·834
·
IF 2.753
Hongxia Su,Chongxing Huang,Ying Liu,Song Kong,Jian Wang,Haohe Huang,Bobo Zhang
In this study, different amounts of cinnamomum essential oil (CEO) were encapsulated in chitosan nanoparticles (NPs) (CS-NPs) through oil-in-water emulsification and ionic gelation. An ultraviolet-visible spectrophotometer, Fourier-transform infrared spectroscopy, synchronous thermal analysis, and X-ray diffraction were employed to analyze the CEO encapsulation. As observed by field-emission scanning electron microscopy, NP size analysis and zeta potential, the prepared CS-NPs, containing CEO (CS-CEO), were spherical with uniformly distributed sizes (diameters: 190–340 nm). The ranges of encapsulation efficiency (EE) and loading capacity (LC) were 4.6–32.9% and 0.9–10.4%, with variations in the starting weight ratio of CEO to CS from 0.11 to 0.53 (w/w). It was also found that the antioxidant activity of the CS-NPs loaded with CEO increased as the EE increased. The active ingredients of the CEO were prevented from being volatilized, significantly improving the chemical stability. The antioxidant activity of CS-CEO was higher than that of the free CEO. These results indicate the promising potential of CS-CEO as an antioxidant for food processing, and packaging applications.Keywords:cinnamomum essential oil;chitosan;encapsulation;antioxidant activity
研究领域:
食品科学
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材料科学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2016
·
IF 1.586
Li-yi Zhao,Jian Chen,Zhi-qiang Wang,Rui-meng Shen,Nan Cui,Ai-dong Sun
Cyanidin-3-glucoside is an anthocyanin that is abundant in blueberry. Lauric acid was used as the acyl donor in the acylation of cyanidin-3-glucoside. Preparative high performance liquid chromatography was used to separate and purify the acylated cyanidin-3-glucoside, the acylated rate was 30.78%. Fourier transform infrared spectroscopy and liquid chromatography mass spectrometry were used to confirm the basic structure of cyanidin-3-glucoside. Lauric acid was combined with cyanidin-3-glucoside. Lauric acid reacted with the glucoside’s primary hydroxyl group and removed a molecule of water, thereby resulting in the obtained structure. The compositions of the unacylated cyanidin-3-glucoside and acylated cyanidin-3-glucoside were compared under different temperatures and under illumination. The effects of different concentrations of additive compounds on the stability of acylated cyanidin-3-glucoside were compared. Stability of acylated cyanidin-3-glucoside was obviously higher than that of the unacylated cyanidin-3-glucoside because of the higher stability of the ester group than the hydroxyl group. The primary hydroxyl esterification of glucoside improved cyanidin-3-glucoside.
研究领域:
食品科学
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植物化学
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Wei Jia,Zibian Fan,Qingyun Shi,Rong Zhang,Xin Wang,Lin Shi
The current study applied an untargeted metabolomics approach by ultra high performance liquid chromatography quadrupole-orbitaltrap high resolution mass spectrometry (UHPLC-Q-Oritrap-MS) to identify the chemical composition of irradiated goat meat and investigate the effect of irradiation on its metabolic profile and meat quality. A total of 103 metabolites were identified as differential metabolites responsible for metabolic changes in irradiated goat meat, which were involved in phenylalanine , tyrosine and tryptophan biosynthesis, phenylalanine metabolism and purine metabolism. Differential metabolites comprising amino acids , nucleotides and their derivatives were determined as the discriminating factors responsible for the meat quality during irradiation. Specifically, the levels of L-phenylalanine, L-isoleucine, L-histidine, guanosine, guanine, creatinine, glutathione and nicotinic acid were increased while inosine 5′-monophosphate (IMP) and guanosine 5′-monophosphate (GMP) were decreased. Overall, except for L-phenylalanine and guanine, other related metabolites significantly decreased with storage. This study contributes to a comprehensive understanding of the effect of irradiation doses and storage time on goat meat metabolism at the molecular level, so as to assess the quality of irradiated goat meat. Satisfactory results with linearity (R 2 > 0.995), precision (RSD less than 8.9%) and recovery (83%-106%) were obtained, demonstrating that the untargeted mebabolomics approach was appropriate for monitoring the changes of small molecular metabolites in irradiated goat meat and irradiation is a feasible method for goat meat preservation .
研究领域:
食品科学
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生物化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD PROCESS ENGINEERING
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2022
·Vol. 46
·期 6
·e14072
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IF 2.889
Jianghong Wu,Min Zhang,Lihui Zhang,Yaping Liu
As one of the five basic tastes, umami has the advantages of making the overall taste of food more harmoniously. Although various umami substances in food have been isolated and identified, in recent years, there are limit studies on the processing technology for improving food umami. Therefore, it is necessary to explore novel efficient umami improvement technologies to meet the urgentment of the food industry. This experiment was designed to study the effect of 0.1% (w/w) sodium bicarbonate (NaHCO 3 ) combined with different power (0, 120, 180, 240, and 300 W) ultrasound treatment on the taste and flavor of chicken broth. Results demonstrated that the chicken broth under the power of 120 W ultrasound treatment combined with 0.1% (w/w) NaHCO 3 was the most umami, and contained the largest amount of IMP. The chicken broth under the treatment of 180 W ultrasound combined with 0.1% (w/w) NaHCO 3 has better flavor and sensory evaluation as the umami improved. In summary, 180 W ultrasound combined with 0.1% (w/w) NaHCO 3 pretreatment is a simple and low-cost processing technology, which is inclined to apply in industry. Practical Applications Compared with the traditional umami improvement method, ultrasound can significantly reduce the cost of food umami-enhancing with the characteristics of high safety, environmental protection, low operating cost, and high instantaneous efficiency. Sodium bicarbonate is an inorganic salt. Moderate intake is beneficial to the body. It can help neutralize stomach acid and supplement sodium for the body. In this study, low-power ultrasound combined with low-concentration of sodium bicarbonate solution was used to cook chicken soup, and the umami taste was improved to a large extent, which provided a novel method for industrial application.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 23
·8437
·
IF 4.927
Rongfei Lu,Jianwei Xu,Zhen Wang,Shaoyong Zhang,Hailong Wang,Hui Xu,Min Lv
Due to the extensive use of agrochemicals resulting in the emergence of pesticide resistance and ecological environment problems, the research and development of new alternatives for crop protection is highly desirable. In order to discover potent natural product-based insecticide candidates, a series of new cholesterol ester derivatives containing cinnamic acid-like fragments at the C-7 position were synthesized. Some derivatives showed potent pesticidal activities. AgainstMythimna separataWalker, compounds2a,Id,Ig, andIIgshowed 2.1–2.4-fold growth-inhibitory activity of the precursor cholesterol. AgainstPlutella xylostellaLinnaeus, compoundsIg,IIf, andIIiexhibited 1.9–2.1-fold insecticidal activity of cholesterol. These results will pave the way for the future synthesis of cholesterol-based derivatives as agrochemicals.Keywords:cholesterol;cinnamic acid;structural modification;agricultural activity
研究领域:
植物保护
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有机化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）