TOXICOLOGY
·
2021
·
IF 4.221
Peipei Qiao,Yingbing Zhang,Ying Yang,Ru Meng,Zhiming Xu,Xianlei Jiang,Yong Zhang,Chengtu Zhang,Jianmin Su
Isoniazid (INH), a synthetic first-line tuberculosis antibiotic, has been widely used in clinical treatment. It has been reported to cause toxic effects at multiple tissue sites and also increases the incidence of adverse pregnancy outcomes; but the mechanism of action of INH on the reproductive system of female mammals remains unclear. Here, we demonstrate that oral INH (40 mg/kg/day every other day for 28 days) severely affects oocyte maturation and fertilization, late blastocyst development and fertility. We found that INH could disrupt standard spindle assembly, chromosome arrangement, and actin filament dynamics, which compromised meiotic progression of mouse oocytes. INH treatment increased the level of reactive oxygen species (ROS) and activated the oxidative stress response pathway, Keap1-Nrf2. It also caused apoptosis of oocytes and mitochondrial dysfunction. Our findings demonstrate that oral INH reduces fertility and damages the mammalian reproductive system by altering cytoskeletal dynamics and Juno expression, inducing oxidative stress and apoptosis, and activating the Keap1-Nrf2 signaling pathway in mouse oocytes.
研究领域:
药理学
·
生殖生物学
·
毒理学
✓ 已验证
DOI
PubMed
高影响因子
Coatings
·
2020
·Vol. 10
·期 4
·347
·
IF 2.436
Yang Liu,Ben Wang,Yuanfeng Wang,Jiareng Chen,Bin Cui,Pengfei Yin,Jianming Chen,Xinyu Zhang,Li Zhang,John H. Xin
Hydrophilic polypyrrole-coated copper nanoneedles (PPy-CuNDs) were synthesized and utilized to construct a superhydrophobic surface on a polyethylene terephthalate fabric (PET) by using the spray-coating technique. The morphology of the as-synthesized PPy-CuNDs can be facilely tuned by changing the concentration of the reducing agent: hydrazine monohydrate. The CuNDs with well-defined nanocrystalline structures and nanoscale thick, rough PPy coating layers were formed simultaneously in one pot. The PPy-CuNDs self-assembled into an entangled, stacking nanocarpet on the surface of the PET fabric, and they eventually formed a reentrant surface texture similar to that of chrysanthemum leaves. The PPy-CuND-PET surface initially showed good superhydrophobic properties, but a fast transition from the superhydrophobic state to the highly adhesive state was observed. The underlying mechanism of this transition and its potential applications were proposed in the context.
研究领域:
材料科学
·
纳米技术
·
表面化学
·
纺织工程
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Chongyang Liu,Yuanyuan Cai,Jin Wang,Xuan Liu,Han Ren,Lu Yan,Yujiao Zhang,Shuqing Yang,Jing Guo,Aihua Liu
Nanozymes are artificial enzymes, which can substitute traditional biological enzymes for multifield applications. However, to date, it remains challenging to search novel mimic enzymes or multienzyme mimics. Herein, a facile and green method for preparing monodisperse, homogeneous copper nanoclusters (Cu NCs) with smaller size was developed, which used cysteamine as a template and hydrazine hydrate as a reductant to reduce Cu2+. The as-prepared Cu NCs exhibited excellent tetraenzyme-like activities, including peroxidase (POD)-, catalase (CAT)-, superoxide dismutase (SOD)-, and ascorbic acid oxidase (AAO)-mimic activities. The mechanisms, kinetics, and catalytic performances of Cu NCs were systematically studied. Moreover, based on the POD-like activity of Cu NCs, sensitive and simple colorimetric sensing glutathione (GSH) was explored, with the low limit of detection of 0.89 μM GSH (S/N = 3). Additionally, a novel fluorimetric ascorbic acid (AA) sensor was developed with the linear range of 0.5–30 μM and limit of detection (LOD) of 0.144 μM, on the basis of the principle that AA is oxidized to dehydroascorbic acid (DHAA) specifically catalyzed by the AAO-like activity of Cu NCs, while DHAA can further react with o-phenylenediamine (OPDA) to generate a highly fluorescent quinoxaline (DFQ) derivative. The as-proposed colorimetric GSH sensor and the fluorimetric AA sensor were capable of detecting GSH and AA, respectively, in real samples accurately and reproducibly. Thus, the Cu NCs-based multienzyme mimic is a promising candidate for biocatalysis and biosensing.
研究领域:
纳米技术
·
生物化学
·
分析化学
·
生物催化
·
生物传感
✓ 已验证
DOI
高影响因子
Frontiers of Chemical Science and Engineering
·
2023
·Vol. 17
·期 10
·1568-1580
·
IF 4.5
Sun Nan,Wu Qing,Jin Lifang,Zhu Zichen,Sun Jianhui,Dong Shuying,Xie Haijiao,Zhang Chunyan,Cui Yanrui
By using a two-step hydrothermal method and trithiocyanuric acid (TTCA), 2,4,6-trihydrazino-1,3,5-triazine (THT), and Fe 3 O 4 as raw materials, a spherical magnetic adsorbent polymer (TTCA/THT@Fe 3 O 4 ) was synthesized to achieve the efficient removal of Cr(VI) from wastewater. Under optimal adsorption conditions, the maximum adsorption capacity of TTCA/THT@Fe 3 O 4 for Cr(VI) can reach 1340 mg·g −1 . Notably, the removal efficiency can approach 98.9%, even at the lower concentration of 20 mg·L −1 Cr(VI). For actual wastewater containing Cr(VI), the Cr(VI) concentration was reduced from 25.8 to 0.4 mg·L −1 , a remarkable 20% lower than the current industry discharge standard value. A mechanism for the high adsorption performance of Cr(VI) on TTCA/THT@Fe 3 O 4 was investigated using Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and density functional theory. It can be plausibly attributed to the formation of Cr/N and Cr/S coordination bonds. Additionally, surface electrostatic adsorption, reduction effects, and the spherical polymer structure increase the contact area with Cr(VI), maximizing adsorption. The synergistic effect of adsorption and reduction enhances the adsorption performance of TTCA/THT@Fe 3 O 4 for Cr(VI) and total chromium in water. The resultant polymer has a simple preparation process, excellent adsorption performance, easy magnetic separation, and promising application for actual wastewater.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
POLYMER DEGRADATION AND STABILITY
·
2022
·
IF 5.204
Yumin Wu,Yizhi Jiao,Zhihao Rong,Chuanhui Gao,Yuetao Liu
Manufacturing organosilicon elastomers that have combined reprocessability, self-healing, and high stretchable properties, this remains a significant challenge. Herein, we proposed a simple one-pot method to synthesize thermoset organosilicon elastomers which could be self-healed and had good mechanical properties. The elastomers were capped with the α,ω-hydroxypolydimethylsiloxane and isophorone diisocyanate, following chain expansion with adipic dihydrazide, and cross-linked by triethanolamine. The average elongation of the elastomers was 1145%, with the largest strain of 4.1 MPa. Due to the highly flexible Si-O chain and the large number of hydrogen bonds formed by the dynamic acyl semicarbazide (ASC) groups, the elastomers had good self-healing ability. It was noted that the elastomers could be recycled entirely under the synergistic dynamic effect of ASC groups without chemical treatment, and the elastomers could achieve 91% of the initial mechanical strength even after ten times of dissolution recycling. And through three times of hot pressing recovery, 86% of the mechanical properties were also maintained. In addition, the self-healing flexible electronic devices were prepared by compounding multi-walled carbon nanotubes (MWCNTs), and they could monitor the subtle movements of the human body. This work fabricated the reprocessable and self-healing organosilicon elastomers for flexible electronic devices, which might have essential inspiration for the sustainable development of organosilicon materials.
研究领域:
材料科学
·
聚合物化学
·
柔性电子
·
可持续材料
✓ 已验证
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Ruixing Zhang,Wenjing Yang,Qiming Pan,Qi Zeng,Chengtai Yan,Xue Bai,Yao Liu,Lugang Zhang,Baohua Li
The aim of this study was to investigate the impact of long-term exposure to blue light-emitting diodes (LEDs) on the accumulation of indolic glucosinolates and carotenoids, as well as the plant growth and antioxidant activities in both orange and common Chinese cabbage ( Brassica rapa L. ssp. pekinensis ). Blue light treatment also induced higher ferric-reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl by 20.66 % and 30.82 % and antioxidant enzyme activities catalase, peroxidase, superoxide dismutase, and the accumulation of non-enzymatic antioxidant substances (total phenols and total flavonoids) in the orange Chinese cabbage. Furthermore, long-term exposure to blue light had negative effects on the net photosynthetic rate and chlorophyll fluorescence levels. Meanwhile, blue light promoted accumulation of Indol-3-ylmethyl glucosinolate (I3M), β-carotene, lutein and zeaxanthin due to the high expression of regulatory and biosynthetic genes of the above metabolic pathways. In particular, lycopene and β-carotene content in orange Chinese cabbage increased by 60.14 % and 65.33 % compared to the ones in common line. The accumulation of carotenoid and increasing antioxidant levels in the orange cabbage line was influenced by long-term blue light irradiation, leading to better tolerance to low temperature and drought stresses. The up-regulation of transcription factors such as BrHY5-2 , BrPIF4 and BrMYB12 may also contribute to the increased tolerance in orange Chinese cabbage to extreme environmental stresses. The BrHY5-2 gene could activate carotenoid biosynthetic genes and induce the accumulation of carotenoids. These findings suggested that long-term blue light irradiation could be a promising technique for increasing the nutrition value and enhancing tolerance to low temperature and drought stresses in Chinese cabbage.
研究领域:
植物科学
·
农业生物技术
·
分子生物学
·
植物生理学
·
抗逆性
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）