JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
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2023
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IF 4.1
Zheyuan Ren,Yang Yu,Zhongyao Ji,Huitao Li,Xiaoheng Li,Han Lin,Renshan Ge,Qiqi Zhu
The potential inhibitory effects of flavonoids on gonadal steroid biosynthesis have gained attention due to their widespread presence in natural plant sources. Specifically, our study focused on evaluating the inhibitory efficacy of these compounds on human 3β-hydroxysteroid dehydrogenase 2 (h3β-HSD2) and rat homolog r3β-HSD1, enzymes responsible for the conversion of pregnenolone to progesterone . Through our investigations, we observed that the potency of flavonoids was silymarin (IC 50 , 1.31 μM) > luteolin (4.63 μM) > tectorigenin > (5.86 μM), and rutin (44.12 μM) in inhibiting human KGN cell microsomal h3β-HSD2. Similarly, the potency of flavonoids was silymarin (9.50 μM) > luteolin (11.49 μM) > tectorigenin (14.06 μM), and rutin (145.71 μM) in inhibiting rat testicular r3β-HSD1. Silymarin, luteolin, and tectorigenin acted as mixed inhibitors of both human and rat 3β-HSDs. Luteolin and tectorigenin were able to penetrate human KGN cells to inhibit progesterone secretion . Furthermore, docking analysis and structure-activity relationship analysis highlighted the importance of hydrogen bond formation for the inhibitory efficacy of these compounds against h3β-HSD2 and r3β-HSD1. Overall, this study demonstrates that silymarin exhibits the most potent inhibition of human and rat gonadal 3β-HSDs, and significant SAR differences exist among the tested compounds.
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Scientific Reports
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2023
·Vol. 13
·期 1
·1-12
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IF 4.6
Huang Huiming,Zou Hui,Lin Hongting,Dai Yimin,Lin Jiangbo
Plants with partial or complete loss of chlorophylls and other pigments are frequently occurring in nature but not commonly found. In the present study, we characterize a leaf color mutant ‘ arly01 ’ with an albino stripe in the middle of the leaf, which is an uncommon ornamental trait in Anoectochilus roxburghii . The albino “mutant” middle portion and green “normal” leaf parts were observed by transmission electron microscopy (TEM), and their pigment contents were determined. The mutant portion exhibited underdevelopment of plastids and had reduced chlorophyll and other pigment (carotenoid, anthocyanin, and flavonoid) content compared to the normal portion. Meanwhile, comparative transcript analysis and metabolic pathways mapping showed that a total of 599 differentially expressed genes were mapped to 78 KEGG pathways, most of which were down-regulated in the mutant portion. The five most affected metabolic pathways were determined to be oxidative phosphorylation, photosynthesis system, carbon fixation & starch and sucrose metabolism, porphyrin and chlorophyll metabolism, and flavonoid biosynthesis. Our findings suggested that the mutant ‘ arly01 ’ was a partial albinism of A. roxburghii , characterized by the underdevelopment of chloroplasts, low contents of photosynthetic and other color pigments, and a number of down-regulated genes and metabolites. With the emergence of ornamental A. roxburghii in southern China, ‘ arly01 ’ could become a popular cultivar due to its unique aesthetics.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
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2018
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IF 3.112
Yurong Ma,Qingfeng Ban,Jingying Shi,Tiantian Dong,Cai-Zhong Jiang,Qingguo Wang
The effects of 1-methylcyclopropene (1-MCP) treatment, storage time, and shelf life and temperature on phenolic concentrations and antioxidant activity in apple peel and flesh of ‘Jonagold’ apple ( Malus domestica Borkh) have been investigated. Hyperoside and chlorogenic acid were the most abundant phenolic compounds in the peel and flesh, respectively. The flesh had lower concentrations of individual phenolics, total phenolics, flavonoids and lower antioxidant activity (determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay) than the peel. In addition, the profiles of phenolics as well as the changing patterns of individual phenolics also differed between the peel and the flesh. After long-time storage (180 d), the greatest decrease of concentrations was the concentrations of hyperoside (27 %) in the peel and total phenolics (35 %) in the flesh. Also, during the 90–180 d storage period, hyperoside and rutin in the peel, chlorogenic acid in the flesh, and flavonoids and antioxidant activity decreased in both peel and flesh tissues. Individual phenolics exhibited different stabilities during the early stages after harvest. 1-MCP reduced the reduction in the concentrations of hyperoside in the peel, chlorogenic acid in the flesh, and total phenolics, flavonoids and antioxidant activity in both the peel and flesh tissues during storage and shelf life. However, for quercetin and rutin, the protective effect of 1-MCP was only observed during the shelf life period. Overall, phenolic compounds responded variously to storage, shelf life, shelf temperature and 1-MCP treatment.
研究领域:
食品科学
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植物生理学
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POLYMER INTERNATIONAL
·
2023
·Vol. 72
·期 6
·586-596
·
IF 3.2
Fangping Liang,Wurong Li,Mingao Li,Xican Li,Jianfeng He,Quanzhou Wu
In this work, kaempferol molecularly imprinted polymers (MIPs) were screened in detail and were applied to the separation of kaempferol from real complex systems. Kaempferol-MIPs with high affinity and selectivity for kaempferol could be prepared successfully by using kaempferol as the template molecule, 4-vinylpyridine as the functional monomer, ethylene glycol dimethacrylate or divinylbenzene as the crosslinker, acetone or acetonitrile as the solvent. Scanning electron microscopy observation and Brunauer–Emmett–Teller measurement showed that the prepared MIPs had a heterogeneous pore structure and large specific surface area. Static and dynamic adsorption experiments and solid-phase extraction application were performed to evaluate the performance of the prepared MIPs. Only the MIPs with both higher distribution coefficient and higher imprinting factor in the dynamic experiments had better ability to retain kaempferol. Using the screened MIPs as solid-phase extraction sorbents, kaempferol and quercetin were extracted directly from the crude ginkgo leaves hydrolysate with high recovery and purity. Control experiments also showed that the screened MIPs were superior to commercially available adsorbents in terms of the separation of kaempferol from crude extracts. © 2023 Society of Industrial Chemistry.
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Food Chemistry-X
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2023
·
IF 6.1
Chengyun He,Lu Bai,Daqun Liu,Benguo Liu
The binding capacity of 10 flavonoids with okra seed protein (OSP) was studied by fluorescence spectroscopy. The structure of flavonoids had an obvious impact on binding performance. The binding ability of flavanone was lower than that of flavone, isoflavone and dihydrochalcone. The binding capacity of flavonoid glycoside was superior to that of the corresponding flavonoid aglycone. The binding ability was positively correlated with the number of phenolic hydroxyl groups on the B ring. The steric field and electrostatic field model constructed by 3D-QSAR method could well explain the above interaction behavior. Thermodynamic analysis suggested that the quenching mechanism of OSP caused by flavonoids was static quenching, and the binding-site number was 1. In addition, hydrogen bonding and van der Waals force dominated this interaction. The 3D and synchronous fluorescence spectra showed that there was no significant change in the polarity of the environment around tryptophan and tyrosine residues during binding.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
·
IF 8.6
Danzhao Guo,Yuying Mao,Xuan Zhang,Bin Bai,Xingchen Yan,Shucheng Liu
In this work, two new kinds of boronic acid functioned sorbents (HCLPH-N 3 @PCAPBA and HCLPH@N 3 -PBA) based on emulsion reactors coupled with click chemistry and boronate affinity were developed for the separation of naringin (NRG). The new clickable boronate ligands 3-(prop-2-ynyloxycarbonylamino) phenylboronic acid (PCAPBA) and azide phenylboronic acid (N 3 -PBA) were utilized as functional monomers for the specific separation and purification of NRG molecules, respectively. The pore structure of macroporous polymers were adjusted via the hypercrosslinking reactions, which can effectively increase their specific surface area and provide abundant recognition sites. The resulting HCLPH-N 3 @PCAPBA and HCLPH@N 3 -PBA exhibited fast equilibrium adsorption at 120 min, and their maximum absorption amounts were 29.85 μmol g −1 and 41.27 μmol g −1 at 303 K, respectively. The HCLPH-N 3 @PCAPBA and HCLPH@N 3 -PBA demonstrated remarkable stability in six cycles of adsorption–desorption experiments (88.48% and 92.02%, respectively). Furthermore, the HCLPH@N 3 -PBA achieved an efficient extraction 93.4% for the commercially available NRG. Our findings provided an idea for constructing hierarchically porous high-surface-area polymers with fast penetrability and various abundant binding sites have great potential for separation and purification of industrial samples.
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CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Shucheng Liu,Nana Tang,Xuan Zhang,Hao Huang,Jinyu Li,Hongxiang Ou
Hydrogel-based imprinted materials with specific-recognition and ultrafast transport have been considered as one of the most promising sorbents in the selective separation fields. Nonetheless, the traditional hydrogel imprinted sorbents still facing the low-level interfacial consistency between substrate polymer and imprinted sites, unordered arrangement and easy agglomeration of recognizing sites on hydrogel matrix, causing poor selectivity of specific molecular transport. For the first time, the one-step RAFT Pickering HIPEs emulsion imprinted methods were designed to fabricate porous hydrophilic boronate affinity imprinted hydrogels (POH-BA-MIPs) for the specific separation and purification of Naringin (NRG). As expected, the novel RAFT solid emulsifier can not only enhance the emulsion stability and mechanical performance of the hydrogel, but also introduce the self-initiating function. Benefiting from the hydrogel’s porous structure and the highly accessible and uniform imprinted sites via Pickering emulsion template, the POH-BA-MIPs exhibited strong binding affinity and selectivity to the targeted molecules. The maximum equilibrium adsorption capacity of POH-BA-MIPs were 53.27 µmol g −1 at 308 K within 360 min. The kinetic nonlinear pseudo-second-order model ( R 2 = 0.997) can well explain the adsorption kinetic than pseudo-first-order model ( R 2 = 0.986), indicating that chemisorption is mainly the rate-limiting step. Besides, the adsorption amounts of POH-BA-MIPs were 3.74 times higher than that of non-imprinted adsorbents (POH-BA-NIPs). After six consecutive adsorption–desorption cycles, the obtained POH-BA-MIPs demonstrated considerable reusability, retaining 91.28 % of the initial adsorption capacity. Meanwhile, the coarse NRG products can be effectively purified to 93.16 %. The obtained purified NRG had better antibacterial performance against Staphylococcus aureus. This work provides a novel strategy for constructing porous boronate affinity imprinted hydrogels for specific capture of targeted flavonoid molecules, which exhibited broad practical application potential.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
·
IF 8.2
Caiying Zhang,Yuelong Li,Abdus Samad,Hongliang He,Huan Ma,Yang Chen,Tengchuan Jin
Kyasanur Forest disease virus (KFDV), a tick-borne flavivirus prevalent in India, presents a serious threat to human health. KFDV NS3 helicase (NS3hel) is considered a potential drug target due to its involvement in the viral replication complex. Here, we resolved the crystal structures of KFDV NS3hel apo and its complex with three phosphate molecules, which indicates a conformational switch during ATP hydrolysis . Our data revealed that KFDV NS3hel has a higher binding affinity for dsRNA , and its intrinsic ATPase activity was enhanced by dsRNA while being inhibited by DNA . Through mutagenesis analysis, several residues within motifs I, Ia, III, V, and VI were identified to be crucial for NS3hel ATPase activity. Notably, the M419A mutation drastically reduced NS3hel ATPase activity. We propose that the methionine-aromatic interaction between residues M419 and W294, located on the surface of the RNA-binding channel, could be a target for the design of efficient inhibitor probes. Moreover, epigallocatechin gallate (EGCG), a tea-derived polyphenol, strongly inhibited NS3hel ATPase activity with an IC 50 value of 0.8 μM. Our computational docking data show that EGCG binds at the predicted druggable hotspots of NS3hel. Overall, these findings contribute to the development and design of more effective and specific inhibitors.
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Journal of Materials Chemistry A
·
2023
·Vol. 11
·期 47
·25875-25886
·
IF 11.9
Shiqin Liu,Mengjia Zhu,Yuxiang Huang,Yanglun Yu,Wenji Yu,Bin Lv
Plant polyphenols are a type of natural substance widely present in plants, which can form three-dimensional metal-phenolic networks (MPNs) via chelation with metal ions, thereby enabling the construction of functional material coatings. Herein, a novel and straightforward approach was employed to prepare superhydrophobic wood by utilizing a nature-inspired strategy. The main component of wood polyphenols, rutin, was employed to create MPNs with various metal ions (Fe(III), Fe(II), Al(III), and Cu(II)), resulting in the formation of a rough hierarchical structure on the wood surface, followed by a polydimethylsiloxane (PDMS) modification. Polyphenol-free wood was impregnated with rutin solution and then complexed with metal ions. Taking rutin-Fe(III)-based superhydrophobic poplar as an example, it showed excellent performance in self-cleaning and anti-fouling. In addition, it showed excellent resistance to acids and alkalis and organic solution impregnation. The superhydrophobic coating maintained robust mechanical durability under the action of long-term placement, ultrasonic washing, tape peeling and sandpaper grinding as well. We believe that the superhydrophobic wood modified with rutin–metal complexes and PDMS is considered environmentally friendly, cost-effective, sustainable, and easily scalable. Thus, it exhibits immense potential for practical applications in indoor and outdoor decoration as well as building materials.
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FOOD CHEMISTRY
·
2023
·
IF 8.8
Zhihui Wang,Yilin Liang,Chenxi Gao,Weiwei Wu,Jiumei Kong,Zhe Zhou,Zhihua Wang,Yan Huang,Weijiang Sun
This study explored the sensory characteristics, metabolites and antioxidant capability of aged Jinhua white tea (AJWT) over different years of aging and revealed the transformation mechanism of these characteristics during the long-term aging process. The flavor wheel of AJWT was constructed, and its unique flavor was dominated by mellowness, smoothness, a fungus fragrance, and a stale flavor. The high content of theabrownine, soluble sugar, flavonoids and 25 aroma components made important contributions to the formation of the unique flavor of the AJWT, and their content significantly increased during the long-term aging process of 5–10 years. This was related to the microbial bioconversion, the oxidative degradation of catechins, the hydrolysis of flavonosides and the decomposition of polysaccharides. Contrary to folk experience, AJWT had weak comprehensive antioxidant capacity, mainly due to its low content of tea polyphenols, catechin components and caffeine, which decreased significantly during the long-term aging of 5–10 years.
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Food Bioscience
·
2023
·
IF 5.2
Jingyu Wang,Lei Zhou,Zixu Wang,Ye Tao,Lin Chen,Wangang Zhang
This study was designed to investigate the effects of natural deep eutectic solvents (NADESs) on extraction efficiency and antioxidant activities of phenolic compounds from Toona sinensis seeds (TSSs) compared with ethanol and water extraction methods. The single factor experiment was employed to optimize the extraction conditions of TSSs phenolic compounds on NADESs. Results indicate that the NADES of choline chloride-oxalic acid (CC-OA) with a molar ratio of 2:1 (solid/liquid ratio: 1:90 (m/v), extraction time: 30 min) showed the highest total phenolic content (32.58 mg GAE/g DW) and antioxidant activities (DPPH radical scavenging activity: 95.94%) compared with ethanol or water extraction. In addition, 15 main phenolic compounds were identified in the extracts of CC-OA solvent, while only 9 and 6 main compounds were identified in the extracts of ethanol and water solvents, respectively. The molecular dynamic simulation result illustrates that the high extraction efficiency of the NADES mainly attributing to their high compatibility as well as hydrogen interaction between ellagic acid (the main polyphenol in TSSs) and CC-OA solvent. In summary, the NADES (CC-OA at the ratio of 2:1) showed superior extraction efficiency on TSSs phenolic compound compared with ethanol or water extraction which could be an environmentally friendly method for the high-value utilization of TSSs.
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Current Research in Food Science
·
2023
·
IF 6.3
Yuchen Shangguan,Jing Ni,Lili Jiang,Yang Hu,Chuanbo He,Ying Ma,Guohong Wu,Hejian Xiong
In this study, flavonoids were extracted from pomelo peels and naringin was isolated from the flavonoid extract. The effects of extraction parameters, namely, ethanol concentration, solid-to-liquid ratio, and extraction time, on the yield of flavonoids extracted from pomelo peels were analyzed according to the Box-Behnken design of response surface methodology. The experimental conditions for flavonoid extraction were optimized, and naringin was separated from the extracted flavonoids using Sephadex LH-20 column chromatography. Experimental results showed that the influence of factors on the extraction rate of flavonoids from pomelo peels was in the order of ethanol concentration > solid-to-liquid ratio > extraction time, and the optimal extraction parameters were 85% ethanol concentration, 1:20 solid-to-liquid ratio, and 4-h extraction time for extracting flavonoids from pomelo peels. Under these conditions, the yield of flavonoids was 6.07 ± 0.06 mg/g. After three times of extraction, the flavonoid extraction rate reached 96.55%, and the residual naringin in the pomelo peels was 0.017 mg/g, at which point the bitterness in the pomelo peels disappeared. Two components, namely, PF1 and PF2, were separated from the crude flavonoid of pomelo peels through Sephadex LH20 column chromatography. PF2 was identified as naringin by high-performance liquid chromatography tandem mass spectrometry, with a purity of 95.7 ± 0.23%. Both flavonoids and PF2 exhibited good in vitro radicals scavenging activities on DPPH, ABTS, superoxide anion and hydroxyl.
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