JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
·
2016
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IF 1.685
Jinxin Che,Junling Shi,Zhenhong Gao,Yan Zhang
An enzymatic reaction system was developed and optimized for bioconversion of resveratrol from glucose. Liquid enzyme extracts were prepared from Alternaria sp. MG1, an endophytic fungus from grape, and used directly or after immobilization with sodium alginate. When the enzyme solution was used, efficient production of resveratrol was found within 120 min in a manner that was pH-, reaction time-, enzyme amount-, substrate type-, and substrate concentration-dependent. After the optimization experiments using the response surface methodology, the highest value of resveratrol production (224.40 µg/l) was found under the conditions of pH 6.84, 0.35 g/l glucose, 0.02 mg/l coenzyme A, and 0.02 mg/l ATP. Immobilized enzyme extracts could keep high production of resveratrol during recycling use for two to five times. The developed system indicated a potential approach to resveratrol biosynthesis independent of plants and fungal cell growth, and provided a possible way to produce resveratrol within 2 h, the shortest period needed for biosynthesis of resveratrol so far.
研究领域:
微生物学
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酶工程
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天然产物化学
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生物转化
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代谢工程
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DOI
PubMed
高影响因子
PHYTOCHEMICAL ANALYSIS
·
2022
·Vol. 33
·期 4
·590-598
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IF 3.024
Xuemei Xue,Fei Deng,Zhenkai Ge,Jingwen Meng,Xiya Liu,Yongheng Zhao,Yanggen Hu,Xu Ling
Introduction The quantitative analysis of trace resveratrol and polydatin in plant tissues is suitable for elucidation of the compounds' mechanisms of action. Objectives The main objective of this work was to develop a feasible and effective sample pretreatment method to measure the concentrations of resveratrol and polydatin in complex samples. Methodology A polymer sorbent, poly(2-mercaptobenzimidazole), was electrochemically prepared and utilized for selective extraction, while resveratrol and polydatin were used as target analytes. The sorbent was characterized by cyclic voltammetry, scanning electron microscopy and Fourier transform infrared spectroscopy. After extraction and elution, the analytes were analyzed by a Thermo U3000 HPLC system. Several affecting parameters, including the volume of elution solution, sample pH value, sample flow rate and sample volume, were evaluated and optimized. Results The proposed method showed good linearity with low limits of detection (from 0.5 to 0.8 ng·mL −1 ) and ideal accuracy with spiked recoveries from 81.30% to 99.16%. A good enrichment factor (more than 200-fold) together with good sensitivity was obtained with this method. Analysis of resveratrol and polydatin in Polygonum cuspidatum samples by this method is efficient. Conclusion The method developed in this work exhibits several significant merits, including easy operation and high extraction efficiency, indicating that electrochemically prepared polymer sorbent is useful for sample pretreatment and analysis of traditional Chinese medicine samples.
研究领域:
分析化学
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中药学
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DOI
高影响因子
INFLAMMATION RESEARCH
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2025
·Vol. 74
·期 1
·1-20
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IF 5.4
Song Chao,Shen Baoxin,Chen Chaoqi,Yang Lei,Zhang Chi,Liu Fei,Chen Feng,Wu Xiaofei
Background Osteoarthritis (OA) is a common chronic degenerative joint disease in orthopedics, and ferroptosis is a newly identified mode of cell death present in OA. Inhibition of inflammatory cytokine expression and modulation of chondrocyte ferroptosis related pathways may be novel strategies for the treatment of OA. The purpose of this work was to uncover prospective biomarkers and molecular processes of ferroptosis in OA, as well as to better understand the molecular mechanisms of ferroptosis in OA treated with resveratrol. Material and methods We obtained OA gene expression profiles from the Gene Expression Omnibus (GEO) database. OA-expressed ferroptosis-related genes were identified using Genecards data, differential gene analysis, and weighted gene co-expression network analysis. Enrichment analysis was utilized to identify signaling pathways and molecular mechanisms linked with ferroptosis in OA, while immune infiltration analysis indicated immune cell infiltration in OA. The action targets of resveratrol were taken from the TCM database to determine the therapeutic targets of resveratrol for the treatment of OA. To validate the molecular process, molecular docking was performed using the therapeutic targets' enrichment analysis. Finally, in vitro investigations confirmed the molecular mechanism of ferroptosis in resveratrol-treated OA. Results Bioinformatic analysis identified 462 OA ferroptosis gene sets, with GPX4, TFRC, SLC7A11, EGFR, and IL1B serving as significant hub genes. Enrichment analysis revealed that ferroptosis was also linked to animal mitophagy, the FoxO signaling pathway, the Toll-like receptor signaling pathway, the PI3K-Akt signaling pathway, inflammation, immune response activation, and cellular autophagy. The immune infiltration data revealed that T_cells_CD4_memory_resting, T_cells_CD4_memory_activated, NK_cells_activated, and Mast_cells_activated were considerably infiltrated in OA. Resveratrol ameliorated OA via modulating autophagy and ferroptosis via GPX4, TFRC, SLC7A11, EGFR, and IL1B, according to a mechanistic study. Conclusion We discovered the mechanism of GPX4, TFRC, SLC7A11, and EGFR, IL1B ferroptosis-related genes in OA, and preliminary evidence suggests that resveratrol improves OA by regulating ferroptosis and immunological processes, which may give a new route for OA treatment.
研究领域:
医学
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生物信息学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
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2025
·Vol. 60
·期 2
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IF 3.1
Zhao Yingna,Chen Zhendong,Yang Yueyue,Lin Pei,Jiao Aiquan,Jin Zhengyu
Polyphenols, as a bioactive components with both anti-inflammatory and enzyme-inhibitory activities, have significant potential for application in regulating abnormal glucose metabolism diseases. Resveratrol, gallic acid, caffeic acid, quercetin, and catechin were analysed for their stability during simulated digestion, their inhibitory effects on starch-digesting enzymes, and their anti-inflammatory properties, revealing that the degradation of free phenolics was related to the environment of the gastrointestinal tract. There existed a quantitative relationship between the inhibitory activity of polyphenols on starch-digestive enzymes, with resveratrol being second only to acarbose in its overall effect. Treatment of cells with 25 mmol/L D-glucose for 24 hr induced glycositis injury in HBZY-1 cells. Compared to the high-glucose group, 5–100 μmol/L resveratrol exhibited significant inhibitory effects on the secretion of inflammatory factors. These findings provide useful information on the use of polyphenols, especially resveratrol, as food-grade ingredients for the prevention and management of diabetic nephropathy.
研究领域:
食品科学
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生物化学
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药理学
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营养学
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分子生物学
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2018
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IF 2.88
Yabo Shi,Min Liu,Hui Yan,Chang Cai,Qingying Guo,Wenxin Pei,Ruiyan Zhang,Zhengping Wang,Jun Han
The individual and combined interactions of bisphenol F and piceatannol with pepsin were investigated using spectroscopic methods (fluorescence, UV–vis absorption, and circular dichroism spectroscopy), combined with isothermal titration calorimetry and molecular docking. Thermodynamic data showed that hydrogen bonds and van der Waals forces might play a major role for the binding process. Site marking experiments and molecular docking confirmed the binding sites of these two ligands on pepsin. The discrepancy in the binding constant between the binary and ternary systems indicated the competitive binding of piceatannol and bisphenol F to pepsin. Circular dichroism spectra studies suggested that the binding of the two ligands led to a loosening of pepsin backbone. Enzyme activity assays indicated that the inhibition of pepsin activity by piceatannol and bisphenol F was competitive. These results will be helpful to understand the mechanism of piceatannol and bisphenol F affecting the activity of digestive proteases in the sight of the food security.
研究领域:
食品科学
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生物化学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Dami Li,Yaoyao Zhang,Xing Qiao,Shangde Sun
Resveratrol monoesters, synthesized by esterifying with lipophilic groups, excel in lipophilicity, antioxidant activity, and bioavailability. This study selectively acylated resveratrol at 3-OH and 4’-OH using lipozyme RMIM (from Rhizomucor miehei ). Seven 3-resveratrol monoesters (3-RC 2:0 – 18:0 ) and seven 4’-RC 2:0 – 18:0 were prepared, purified and identified. Their antioxidant capacity and bioaccessibility were innovatively studied, focusing on substitution and acyl chain length effects. Results showed that 3-RC 2:0 – 18:0 consistently outperformed 4’-RC 2:0 – 18:0 in SET assays for antioxidant activity. Notably, 4’-RC 2:0 – 8:0 performed better oxygen radical absorption capacity than 3-RC 18:0 . 3-RC 2:0 even showed better ABTS radical scavenging capacity than Trolox and TBHQ. 3-RC 2:0 /RC 4:0 displayed higher antioxidant efficacy than medium- and long-chain counterparts, excluding 3-RC 16:0 . In the gastric and intestinal phases, 4’-RC 2:0 – 18:0 showed slightly higher retention due to enhanced lipophilicity compared to 3-RC 2:0 – 18:0 . RC 2:0 /RC 12:0 – 18:0 showed better retention properties during digestion. In summary, 3-RC 2:0/16:0 exhibited exceptional antioxidant capacity and digestive stability. These findings suggest resveratrol derivatives' potential in lipid-based foods, pharmaceuticals, and cosmetics.
研究领域:
食品科学
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营养学
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药理学
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产品关联
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2025
·
IF 12.4
Qiyong Jiang,Meng Liu,Zhaosheng Wang,Rentang Zhang,Yuxiao Wang,Fengjun Guo,Qing Sun
Radical mediated reaction method was chosen to prepare ferulic acid grafted pectin (FA-P) to enhance the properties of the native pectin. HPLC and FTIR results presented that ferulic acid was successfully grafted onto the pectin molecule. Among them, FA-P conjugation with a mass ratio of 0.75:1 was the most effective with the highest grafting efficiency, emulsifying activity index and emulsifying stability index. Subsequently, FA-P (0.75:1) was used to emulsify resveratrol to prepare FA-P-R emulsion. Compared to the resveratrol emulsion emulsified with native pectin (P-R), FA-P-R had a lower ζ-potential and a 57.4% reduction in mean particle diameter. Scanning electron microscopy demonstrated that the particles of FA-P-R emulsion were more rounded, with flatter and smoother surfaces, suggesting the formation of a better emulsified morphology. In addition, the encapsulation efficiency, load capacity and light stability of FA-P-R increased by 31.6%, 31.5% and 28.0%, respectively, compared to P-R. The FA-P-R samples showed the best antioxidant properties among the four emulsions of FA-P-R, P-R, FA-P and pectin for the functional coatings prepared, with the highest DPPH and ABTS + radical scavenging activity. Finally, the FA-P-R functional coating for wrapping frozen limes was able to significantly inhibit the yellowing of frozen limes during freezing storage. The results highlight that the prepared FA-P-R functional coating can be used to inhibit the quality deterioration of frozen fruits and extend the freezing period.
研究领域:
食品科学
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生物化学
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产品关联
DOI
高影响因子
NEUROSCIENCE LETTERS
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2024
·
IF 2.5
Jinyun Long,Xiukuan Li,Chunyan Yao,Xiaoling Liu,Na Li,Yumeng Zhou,Dawei Li,Shengquan Su,Liangmei Wang,Hao Liu,Ying Xiang,Long Yi,Yao Tan,Peng Luo,Tongjian Cai
Lipopolysaccharide (LPS) is an important neurotoxin that can cause inflammatory activation of microglia. ZC3H12D is a novel immunomodulator, which plays a remarkable role in neurological pathologies. It has not been characterized whether ZC3H12D is involved in the regulation of microglial activation. The aim of this study was to investigate the role of ZC3H12D in LPS-induced pro-inflammatory microglial activation and its potential mechanism. To elucidate this, we established animal models of inflammatory injury by intraperitoneal injection of LPS (10 mg/kg). The results of the open-field test showed that LPS caused impaired motor function in mice. Meanwhile, LPS caused pro-inflammatory activation of microglia in the mice cerebral cortex and inhibited the expression of ZC3H12D. We also constructed in vitro inflammatory injury models by treating BV-2 microglia with LPS (0.5 μg/mL). The results showed that down-regulated ZC3H12D expression was associated with LPS-induced pro-inflammatory microglial activation, and further intervention of ZC3H12D expression could inhibited LPS-induced pro-inflammatory activation of microglia. In addition, LPS activated the TLR4-NF-κB signaling pathway, and this process can also be reversed by promoting ZC3H12D expression. At the same time, the addition of resveratrol, a nutrient previously proven to inhibit pro-inflammatory microglial activation, can also reverse this process by increasing the expression of ZC3H12D. Summarized, our data elucidated that ZC3H12D in LPS-induced pro-inflammatory activation of brain microglia via restraining the TLR4-NF-κB pathway. This study may provide a valuable clue for potential therapeutic targets for neuroinflammation-related injuries.
研究领域:
神经科学
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免疫学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Huan-Huan Xiao,Xue-Ying Li,Ying-Ying Chen,Jin-Xiang Zhou,Ting Zhang,Qiang-Ming Li,Li-Hua Pan,Jian-Ping Luo,Xue-Qiang Zha
The aim of this study was to identify novel antioxidant peptides from quinoa ( Chenopodium quinoa wild.) protein hydrolysates, which were prepared using enzyme combination methods. Eleven novel antioxidant peptides were screened through in silico evaluation, and their structure-activity relationships were further investigated. Molecular docking studies indicated that GPGGGGKGEMF (GF-11) exhibited a higher binding affinity and interaction capacity. GF-11 significantly reduced reactive oxygen species (ROS) levels to 43.20 and malondialdehyde (MDA) levels to 1.92 nmol/mg protein in oxidatively damaged RAW264.7 cells, while also significantly enhancing SOD, GSH-Px, and CAT activities to 25.33, 133.99, and 43.20 U/mg protein, respectively. Furthermore, GF-11 competed with Nrf2 for the binding site on Keap1, promoting the expression of Nrf2, HO-1, and NQO1 proteins, which ultimately activated the Keap1-Nrf2 pathway and mitigated oxidative damage. These findings provide a scientific basis for the application of quinoa antioxidant peptides in the development of functional foods.
研究领域:
食品科学
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分子生物学
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营养学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CARDIOVASCULAR PHARMACOLOGY
·
2020
·
IF 2.598
Kaijie Wang,Jinping Zhao,Wenwen Zhang,Minglin Zhu,Ming Xu,Dan Li,Hongjie Shi,Ming Zhang,Jiajun Shi,Zhe Dong
Aortic dissection (AD) is a serious condition and a health issue on a global scale. β-Aminopropionitrile–induced AD in mice is similar to the pathogenesis of AD in humans. Resveratrol (RSV) is a natural polyphenolic substance that provides anti-inflammatory and cardiovascular effects, but the role of RSV in AD is unclear. In this study, we investigated the effects and mechanisms of RSV on β-aminopropionitrile–induced AD in mice. Our results indicate that RSV can prevent the occurrence of AD. More meaningfully, we found that the protective effect comprises an increase in sirtuin 1 (SIRT1) expression in endothelial cells for the reconstruction of their structure, reducing the recruitment of inflammatory cells by endothelial cells and inhibiting the inflammation response, thereby suppressing the occurrence of AD.
研究领域:
心血管疾病
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药理学
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分子生物学
✓ 已验证
DOI
PubMed
高影响因子
NEUROREPORT
·
2025
·
IF 1.7
Zhou Shinan,Liang Lu,Zhong Wenyan,Chen Jingjing,Xiao Li
Excessive neuroinflammation resulting from chronic alcohol intake is an important risk factor for central nervous system injury. The aim of this study was to investigate the effect of kaempferol (KAE) on alcohol-induced neural injury and its underlying mechanism. C57BL/6 N mice were employed to develop a binge-on-chronic alcohol exposure model, with different doses of KAE as an interventional drug for 6 weeks. Neuronal damage and microglial activation in the brain, as well as colonic tissue damage and serum lipopolysaccharide (LPS) concentrations, were systematically assessed. Additionally, Caco-2 cells were exposed to alcohol to induce intestinal epithelial injury in vitro. Chronic alcohol exposure let to significant neuronal damage in the cortex and hippocampus of mice. KAE treatment effectively attenuated microglial activation and reduced neuronal damage in the brains of alcohol-exposed mice. Analysis of colonic tissues revealed that KAE administration inhibited miRNA-122a expression, alleviated pathological damage, and enhanced occludin expression, thereby significantly lowing serum LPS concentrations in alcohol-fed mice. In vitro, KAE markedly decreased miRNA-122a expression and enhanced occludin levels in Caco-2 cells treated with alcohol. Furthermore, overexpression of miRNA-122a was found to diminish occludin protein production in Caco-2 cells, which was significantly counteracted by KAE treatment. KAE treatment enhanced intestinal barrier function to alleviate neuronal damage caused by microglial activation mediated by gut-derived LPS under alcohol expose. This effect of KAE was involved in the enhance of intestinal occludin expression by inhibiting the expression of miRNA-122a. This suggested that KAE had the potential to prevent alcohol-induced neurological damage.
研究领域:
神经科学
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药理学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
STARCH-STARKE
·
2024
·
IF 2.6
Liuyang Xiao,Jingru Zhang,Yumin Huang,Xiaofan Yang,Zhaojun Wei,Lihong Han
Very small granule starch is a good source for effective Pickering emulsion stabilizers. In this study, native Agriophyllum squarrosum ( A. squarrosum ) starch (N-AS) and native quinoa starch (N-QS) are modified by octenyl succinic anhydride (OSA), and the physicochemical as well as the emulsifying characteristics of two modified starches are evaluated. Fourier transform infrared spectroscopy (FTIR) shows that the esterification reactions between OSA and the two starches are successful, and the substitution effect of OSA-modified A. squarrosum starch (OSA-AS) is better than that of OSA-modified quinoa starch (OSA-QS). X-ray diffraction (XRD) analysis shows that OSA modification does not alter the crystal type of the starch particles. OSA modification increases the particle size and hydrophobicity of both starches, and the particles of OSA-AS are smaller than that of OSA-QS, which corresponded to the fact that N-AS possesses a smaller particle size compared to native N-QS. OSA-modified starches are arranged at the interface of oil–water to stabilize the emulsion. The OSA-AS displays better performance of stabilizing emulsion than OSA-QS. The Pickering emulsions formed by all OSA-modified starch samples are mainly elastic gel-like emulsions. In summary, OSA-AS is more suitable for Pickering emulsion stabilizer than OSA-QS.
研究领域:
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）