Journal of Food Measurement and Characterization
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2021
·Vol. 15
·期 5
·3873-3883
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IF 2.431
Qian Zheng-ming,Huang Qi,Feng Xiao-long,Li De-qiang
Given the safety of synthetic antioxidants, people are increasingly inclined to develop and use natural antioxidants. However, rapid screening and identification of active ingredients from the complex systems of natural products is one of the major challenges for the modernization of traditional Chinese medicine. In this study, online extraction-high performance liquid chromatography-diode array detection-quadrupole time-of-flight mass spectrometry coupled with ABTS antioxidant assay (OLE-HPLC-DAD-QTOF-MS/MS-ABTS), in which sample preparation, separation, detection and antioxidant assay were integrated in a single step, was used to rapidly identify antioxidant components from the different parts of Citrus reticulata , including its peels, leaves, vascular bundies and seeds. After method validation (system adaptability, extraction yield and repeatability), the four samples of different botanical parts were analyzed. As a result, among 49 found chromatographic peaks, 33 peaks displayed varying degrees of antioxidant activity and 39 peaks were tentatively or unambiguously identified based on their retention time, UV spectrum and mass spectrometry information and the comparison with reference substance and data in literature, including 10 phenolic acids, 9 polymethoxy flavonoids, 7 flavonoid- O -glycosides and 13 other ingredients. It is concluded that the established method is simple, fast, and reliable, which not only saves the materials and solvents as well as time, but also reduces the risk of degradation of chemical components during the extraction process, suitable for rapid screening and identification of antioxidant components of complex mixture.
研究领域:
生药学
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分析化学
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天然产物
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中药学
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DOI
高影响因子
CATALYSIS LETTERS
·
2019
·Vol. 150
·期 6
·1641-1649
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IF 2.372
Luo Qianqian,Lu Tianliang,Xu Jun,Yang Xiaomei,Zhou Lipeng
Abstract Methyl pyruvate (MPA) was synthesized from dihydroxyacetone (DHA), the oxidation product of glycerol, over Sn-β and TS-1 hybrid molecular sieves at low temperature. Sn-β and TS-1 provide the active sites for conversion of DHA to methyl lactate (MLA) and oxidation of MLA to MPA, respectively. Synergism of Lewis acid sites on Sn-β and TS-1 realize the production of MPA from DHA. After optimization, 71% yield of MPA can be obtained under 50 °C. This is the first report for the synthesis of MPA from DHA directly. Graphic Abstract
研究领域:
催化
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绿色化学
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化工
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DOI
高影响因子
BIOPHYSICAL CHEMISTRY
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2022
·
IF 3.628
Xuejiao Gao,Sujuan Wang,Jiawei Dong,Jie Li,Yuangong Zhang,Yuxia Wu,Xinwu Ba
Curcumin has attracted more attention because of its inhibition efficacy on protein amyloid fibrillation. However, the inhibition mechanism was still ambiguous and the clinical application of curcumin was greatly limited because of its poor stability at physiological conditions for the presence of β-diketone moiety. In this paper, a new mono-ketone-containing curcumin analogue (MDHC) was designed and synthesized to realize the possible inhibition mechanism and unveil the important role of β-diketone moiety of curcumin in the inhibition process of amyloid fibrillation using hen egg white lysozyme (HEWL) as model protein. Although all experiment results (ThT, CR, ANS and TEM) showed that the inhibitory capacity of curcumin was better than MDHC, MDHC still could show obvious inhibition effect. Molecular docking showed that both curcumin and MDHC could bind with HEWL by hydrogen bond of phenloic hydroxyl and the binding energy of MDHC was higher than that of curcumin. All the findings inferred that β-diketone group was one of great important groups in the inhibition process of HEWL amyloid fibrillation, which provided more room to construct novel inhibition reagents.
研究领域:
生物化学
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分子生物学
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药物化学
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2022
·
IF 16.744
Ziqi Zhao,Shaokun Liang,Chengjun Kang,Zhaoqiang Zhang,Liqin Zhou,Zhenxia Zhao,Xingzhen Qin,Yushan Pang,Hongbing Ji,Kungang Chai
Adsorption is considered as a promising method to purify the mixed acetophenone (AP) and 1-phenylethanol (1-PE) that are generally difficult to separate. Many efforts have been made to improve the preferential uptake of AP over 1-PE in aqueous phase, but there exists a practical bottleneck associated with their limited aqueous solubility. Herein, we demonstrate unprecedented reversal of selectivity in the organic medium enabled by β-ketoenamine-linked covalent organic frameworks (COFs), which were fabricated successfully by polycondensation between 1,3,5-triformylphloroglucinol (Tp) and diamines. The dependence of selective adsorption ability toward 1-PE on pore size was firstly investigated experimentally, indicating that TpBZ-COF with moderate pore size outperformed other analogues. The adsorption behaviors of 1-PE and AP onto TpBZ-COF were examined systematically. The evaluation results revealed a strong affinity toward 1-PE versus AP as well as excellent stability in recycling experiments. Further, the dynamic breakthrough experiments confirmed the practical application potential and remarkable recyclability. To probe the mechanism and structure–activity relationship, an imine-linked COF possessing similar porous structure was used as a reference for comparison of separation performance both experimentally and theoretically. The occurrence of hydrogen bonding between the hydroxyl of 1-PE and β-ketoenamine-linkage as H-bond acceptor in suitable pore spaces endows TpBZ-COF a fantastic candidate for purifying 1-PE and AP from petrochemical by-products.
研究领域:
化学工程
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材料科学
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产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
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IF 5.279
Hao-Min Song,Li-Xia Zhao,Shuai-Qi Zhang,Tong Ye,Ying Fu,Fei Ye
Cinnamic acid, isolated from cinnamon bark, is a natural product with excellent bioactivity, and it effectively binds with cyclohexanedione to form novel 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors. According to the active sub-structure combination principle, a series of novel 3-hydroxy-2-cinnamoyl-2-en-1-one derivatives were designed and synthesized. The title compounds were characterized by infrared, 1H NMR, 13C NMR, and HRMS. The in vitro inhibitory activity of AtHPPD verified that compound II-13 showed the most activity with a half-maximal inhibitory concentration (IC50) value of 0.180 μM, which was superior to that of mesotrione (0.206 μM) in vitro. The preliminary herbicidal activity tests demonstrated that some compounds had good herbicidal activity especially compound II-13 at a concentration of 150 g ai/ha. The binding mode of AtHPPD through molecular docking indicated that two oxygens of compounds II-13 formed bidentate interactions with metal ions, and the benzene ring formed π-π accumulation effects with Phe-381 and Phe-424. The results of molecular dynamics simulations showed that compound II-13 exhibited a more stable binding ability with AtHPPD than mesotrione. This study provided insights into the development of natural and efficient herbicides in the future.
研究领域:
化学
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药理学
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农业科学
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分子生物学
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植物科学
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DOI
PubMed
高影响因子
DESALINATION
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2025
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IF 9.8
Hongxiang Zhang,Xing Lai,Jian You,Wei Wang,Meihua Wu,Longmin Liu,Huaiyin Chen,Zhangsheng Luo,Huifei Zhu,Yonghao Wang,Yuekun Lai,Weilong Cai
The growing complexity of dyeing and printing effluents has presented considerable challenges for their effective treatment and recycling. Recently, there has been a notable increase in the development of loose nanofiltration (NF) membranes, which are characterised by their exceptional environmental resilience, high throughput, and superior dye/salt selectivity. This work presents the development of a new loose NF membrane prepared from a modified polymer called poly(ether sulfone ether ketone ketone) (PESEKK). The membrane features a dense-loose-support structure and is prepared through a straightforward one-step, non-solvent induced phase separation (NIPS) technique, eliminating the need for heating or post-treatment. Precise regulation of membrane pore size down to 4.27 nm can be achieved through modulation of PESEKK and pore-former composition. The membranes could separate positively and negatively charged dyes in mixed dye solutions accurately and selectively. Additionally, the membrane demonstrated exceptional dye/salt selective separation with a high water flux of 230.7 L m −2 h −1 bar −1 , Congo red rejection of 99.91 ± 0.13 %, NaCl rejection of 2.71 ± 0.23 %, and a separation factor (α) of 347.4, outperforming state-of-the-art membranes. Remarkably, PESEKK membrane maintained outstanding stability and separation performance for up to 200 h under extreme environments, including NaOH solution (up to 9 mol L −1 ), NaClO solution (up to 40,000 mg L −1 h), n-hexane, isopropanol, and methanol. This high-performing loose NF membrane, developed using innovative polymer materials, offers a promising solution for treating the challenging wastewater generated by printing and dyeing processes.
研究领域:
材料科学
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环境工程
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DOI
高影响因子
Green Processing and Synthesis
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2019
·Vol. 8
·期 1
·568-576
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IF 1.128
Yingna Cui,Changping Li,Ming Bao
Deep eutectic solvents (DESs) are successfully used as powerful and recyclable catalysts and solvents for the synthesis of 3,4-dihydropyrimidin-2(1 H )-ones/thiones (DHPMs). The acidity of DESs is the main factor that determines catalytic activity. DESs, based on p -toluene sulfonic acid (PTSA) and choline chloride (ChCl), exhibits the highest catalytic activity. ChCl/2PTSA is suitable for a vast variety of aromatic aldehydes with electron-donating and electron-withdrawing groups, different β-diketonates, and urea or thiourea to obtain the corresponding DHPMs. Furthermore, DESs can be recycled easily after synthesis. The reused DESs achieve catalytic efficiency six times without significant changes. This study will provide a new green catalyst and efficient process for the synthesis of DHPMs.
研究领域:
有机化学
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绿色化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2012
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IF 3.898
Shujuan Xu,Yong Shao,Kun Ma,Qinghua Cui,Guiying Liu,Fei Wu
Many efforts have been made for the developments of aptamers due to their wide applications for various targets. In this work, we tried to use an abasic site (AP site) that was embeded in a double-stranded DNA as a binding pocket for fisetin . With the AP site being wholly opposed and flanked by thymines (named T-T aptamer), the aptamer exhibits high binding selectivity and sensitivity for fisetin over the other flavonoids such as morin, rutin , apigenin , kaempferol , myricetin , quercetin , luteolin , baicalin , naringenin , genistein , chrysin , and galangin . Upon binding to the AP site, fisetin experiences a significant enhancement in fluorescence emisson by favoring the occurrence of its excited-state intramolecular proton transfer (ESIPT) reaction. The detection limit is about 50 nM at a signal-to-noise ratio of 3. Therefore, the realization for fisetin's selective analysis is beneficial from the novel aptamer design without any fluorophore modification. Thus, the almost negligible fluorescence background from the aptamer is achieved in our method.
研究领域:
生物化学
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分析化学
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药物化学
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DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
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2024
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IF 3.3
Nan Wang,Yuanyuan Huang,You Zi,Mengke Wang,Weichun Huang
A practical and regioselective direct N-alkylation of 2-pyridones is enabled by use of α-keto esters in the P(NMe2)3-mediated deoxygenation process. The reaction proceeds under mild conditions to produce N-alkylated 2-pyridones with high selectivity and generality, and the protocol is shown to be applicable for the scale-up synthesis, which makes it promising for practical applications.
研究领域:
有机化学
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合成化学
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DOI
PubMed
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
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2025
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IF 5.4
Panpan Chen,Xueqing Ding,Junqing Tu,Yi Wang
Sulcotrione and mesotrione are novel plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors. The overuse of these herbicides can bring the risk to environment and human. In this study, multispectral methods, density functional theory (DFT) calculations, and molecular docking techniques were used to investigate how sulcotrione and mesotrione interact with human serum albumin (HSA) and bovine serum albumin (BSA). The results revealed that both herbicides quenched the endogenous fluorescence of HSA/BSA through a static quenching mechanism. Circular dichroism (CD), Fourier Transform Infrared (FTIR), synchronous fluorescence, and three-dimensional (3D) fluorescence spectra demonstrated that sulcotrione and mesotrione induced conformational and secondary structural changes in HSA and BSA. Thermodynamic studies indicated that hydrogen bonding and van der Waals forces were the primary driving forces behind these interactions. Additionally, both compounds enhanced the esterase activity and antioxidant capacity of HSA and BSA while altering amino acid residues. The in vivo toxicity test and ROS levels results of zebrafish also showed that the toxicity of mesotrione was higher than that of sulcotrione. This study provides critical insights into the potential health risks posed by HPPD inhibitors to humans and mammals, emphasizing the need for further safety evaluations.
研究领域:
生物化学
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药物代谢
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分子生物学
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环境毒理学
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结构生物学
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产品关联
DOI
PubMed
高影响因子
COMPOSITES PART B-ENGINEERING
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2025
·
IF 14.2
Xingyao Liu,Xiaoqing Sun,Peifeng Feng,Xinyu Fan,Zhongwei Yan,Xigao Jian,Yujie Song,Jian Xu
Carbon fiber-reinforced thermoplastic resin composites (CFRTPs) have gained significant recognition in industries such as rail transportation, aerospace, and wind power generation. Among these composites, carbon fiber/poly(phthalazinone ether nitrile ketone) (CF/PPENK) composite is considered to be a high potential CFRTP because of the exceptional thermal stability and solubility of its matrix. However, due to the smooth surface of CF and the limited number of reactive groups in PPENK, the CF/PPENK composite demonstrated poor interfacial properties. In this study, drawing inspiration from the strong adhesive properties of barnacle structures, three different interfacial phases were fabricated on the CF surface. In these composites, polydopamine (PDA) particles acted as “barnacle glue” and three types of polyhedral oligomeric silsesquioxane (POSS) acted as “barnacles”. The practicality and effectiveness of the design was initially verified by molecular dynamics simulations, which predicted optimal interfacial properties of the composite with PDA and 3-glycidyloxypropyl-POSS (EP) on the fiber surface (CF-PDA-EP/PPENK). X-ray photoelectron spectroscopy and scanning electron microscopy analyses confirmed the successful combination between the “barnacle structure” and CF. The experimental results aligned well with the simulation outcomes, validating that the interfacial properties of CF-PDA-EP/PPENK were optimal. Compared to the composites with unmodified fibers, the interlaminar shear strength and interfacial shear strength of CF-PDA-EP/PPENK were enhanced by 55.56 % and 210.73 %, respectively. This method offers an efficient and straightforward approach to the interfacial modification of the composites.
研究领域:
材料科学
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复合材料工程
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产品关联
DOI
高影响因子
PLoS One
·
2021
·Vol. 16
·期 4
·e0249915
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IF 3.24
Jing Han,Ze-Tian Qin,Jing Zhang,Wen-Qiang Wang,Jing-Ya Wu,Yong-Ze Lu,Li-Wei Sun
Ultraviolet (UV) filters are used in cosmetics, personal care products and packaging materials to provide sun protection for human skin and other substances. Little is known about these substances, but they continue to be released into the environment. The acute toxicity of 4,4’-dihydroxybenzophenone, 2,4,4’-trihydroxybenzophenone and 4-MBC to Chlorella vulgaris and Daphnia magna were analyzed in this study. The 96 h-EC 50 values of 4,4’-dihydroxybenzophenone, 2,4,4’-trihydroxybenzophenone and 4-MBC on C . vulgaris were 183.60, 3.50 and 0.16874 mg/L, respectively. The 48 h-LC 50 of 4,4’-dihydroxybenzophenone, 2,4,4’-trihydroxybenzophenone and 4-MBC on D . magna were 12.50, 3.74 and 0.54445 mg/L, respectively. The toxicity of a mixture of 4,4’-dihydroxybenzophenone and 4-MBC showed addictive effect on C . vulgaris , while the toxicity of mixtures of 4,4’-dihydroxybenzophenone and 2,4,4’-trihydroxybenzophenone, 2,4,4’-trihydroxybenzophenone and 4-MBC as well as 4,4’-dihydroxybenzophenone, 2,4,4’-trihydroxybenzophenone and 4-MBC all showed antagonistic effect on C . vulgaris . The induced no-effect concentrations of 4,4’-dihydroxybenzophenone, 2,4,4’-trihydroxybenzophenone and 4-MBC by the assessment factor (AF) method were 0.0125, 0.00350 and 0.000169 mg/L, respectively.
研究领域:
环境科学
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毒理学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）