JOURNAL OF ORGANIC CHEMISTRY
·
2021
·
IF 4.354
Chunfang Yang,Chunshuai Huang,Chunyan Fang,Liping Zhang,Siqiang Chen,Qingbo Zhang,Changsheng Zhang,Wenjun Zhang
Inactivation of the flavoenzyme-encoding gene flsO1 in fluostatin biosynthesis led to the isolation of four new angucyclinone derivatives (11, 12, 14, and 15), among which fluostarenes A (14) and B (15) featured the unprecedented 6/6/5/6/6 pentacyclic skeleton with fusion of a benzo[b]fluorene and a six-membered lactone ring. Both 14 and 15 were putatively generated via quinone methide-mediated nonenzymatic reactions. Fluostarene B (15) exhibited cytotoxicity against several cancer cell lines with IC50 values ranging from 7 to 10 μM. Fluostarenes A (14), B (15), and PK1 (16) showed α-glucosidase inhibition activity with IC50 of 0.89, 1.58, and 0.13 μM, respectively. Successful complementation of the ΔflsO1 mutant with alpK from kinamycin biosynthesis suggests that FlsO1 should function equivalently to AlpK as a putative C-5 hydroxylase.
研究领域:
药理学
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生物化学
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分子生物学
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微生物学
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DOI
PubMed
高影响因子
PHYTOCHEMISTRY
·
2024
·
IF 3.2
Kaixuan Wang,Huihui Zeng,Yiqun Dai,Zixuan Wang,Huanying Tang,Junde Li,Xingchen Lu,Neng Jiang,Guoyong Xie,Yan Zhu,Yucheng Zhao,Minjian Qin
Furanocoumarins (FCs) are widely distributed secondary metabolites found in higher plants, including Apiaceae, Rutaceae, Moraceae, and Fabaceae. They play a crucial role in the physiological functions of plants and are well-known for their diverse pharmacological activities. As a representative plant of the Apiaceae family, Angelica sinensis is highly valued for its medicinal properties and FCs are one of the main ingredients of A. sinensis . However, the biosynthetic mechanism of FCs in A. sinensis remains poorly understood. In this study, we successfully cloned and verified three types of enzymes using genome analysis and in vitro functional verification, which complete the biosynthesis of the FCs core skeleton in A. sinensis . It includes a p -coumaroyl CoA 2′-hydroxylase ( AsC2′H ) responsible for umbelliferone formation, two UbiA prenyltransferases ( AsPT1 and AsPT2 ) that convert umbelliferone to demethylsuberosin (DMS) and osthenol, respectively, and two CYP736 subfamily cyclases ( AsDC and AsOD ) that catalyze the formation of FCs core skeleton. Interestingly, AsOD was demonstrated to be a bifunctional cyclase and could catalyze both DMS and osthenol, but had a higher affinity to osthenol. The characterization of these enzymes elucidates the molecular mechanism of FCs biosynthesis, providing new insights and technologies for understanding the diverse origins of FCs biosynthesis.
研究领域:
植物生物化学
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药理学
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分子生物学
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基因组学
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMICAL ENGINEERING
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2020
·
IF 2.627
Jing Wang,Yongqin Lv
Ultrathin polydopamine microcapsules with hierarchical structure and porosity were prepared for the immobilization of multienzymes using metal-organic framework (MOF) as the template. The multienzyme/MOF composite was first prepared using a “one-pot” co-precipitation approach via the coordination and self-assembly of zinc ions and 2-methylimidazole in the presence of enzymes. The obtained nanoparticles were then coated with polydopamine thin layer through the self-polymerization of dopamine under alkaline condition. The polydopamine microcapsules with an ultrathin shell thickness of ~ 48 nm were finally generated by removing the MOF template at acidic condition. Three enzymes were encapsulated in PDA microcapsules including carbonic anhydrase (CA), formate dehydrogenase (FateDH), and glutamate dehydrogenase (GDH). FateDH that catalyzed the main reaction of CO 2 reduction to formic acid retained 94.7% activity of equivalent free FateDH. Compared with free multienzymes, the immobilized ones embedded in PDA microcapsules exhibited 4.5-times higher of formate production and high catalytic efficiency with a co-factor-based formate yield of 342%.
研究领域:
化学工程
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材料科学
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生物化学
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环境科学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Rui Liu,Gaoyi Yi,Baian Ji,Xiuqiong Liu,Yuanqi Gui,Zhining Xia,Qifeng Fu
Enzyme assays are important for studying enzyme-mediated biochemical reactions and for clinical diagnosis and drug development. The technique of an immobilized enzyme microreactor (IMER) integrated with capillary electrophoresis (CE) has been frequently utilized in online enzyme assays. However, the traditional approaches for IMER-CE enzyme analysis have some defects such as low loading capacity and poor stability. Herein, metal–organic frameworks (MOFs), which have enormous potential in the fields of enzyme immobilization and capillary electrochromatographic (CEC) separation, were first explored as novel support materials with good enzyme immobilization performance and stationary phases with excellent separation abilities to construct an integrated MOFs-IMER-CEC microanalysis system for a high-efficiency online enzyme assay. As a proof-of-concept demonstration, acetylcholinesterase (AChE) was immobilized on a densely packed UiO-66-NH2 nanocrystal coating on a capillary inner surface with abundant intercrystalline mesoporosity and was employed as a highly effective and robust IMER for CEC-integrated online enzyme analysis. The excellent separation performance of the UiO-66-NH2-modified capillary was verified by high-efficiency separation of three types of neutral, acidic, and basic compounds. The Michaelis–Menten constant and enzyme inhibition kinetics of UiO-66-NH2–IMER were systematically assessed, exhibiting distinct advantages such as remarkably increased enzyme loadability, superior affinity for substrates, and greatly improved stability and repeatability compared to CE-integrated IMERs prepared by the traditional covalent bonding method. Furthermore, the developed method was successfully utilized for detecting organophosphorus pesticides in leguminous vegetable samples, demonstrating its strong practicality. The study not only proposed a novel support material and construction strategy for a high-performance microchannel-based IMER but also can be widely used in bioanalysis and biosensing research.
研究领域:
分析化学
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生物化学
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生物技术
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环境科学
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产品关联
DOI
PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2023
·
IF 3.9
Shiming Tang,Yukun Chen,Daocheng Liao,Ying Lin,Shuangyan Han,Suiping Zheng
As an essential chemical, p- hydroxystyrene has attracted much attention and is widely used in the field of semiconductors. However, the current production methods have some disadvantages, such as low molar conversion yield, p- hydroxystyrene toxicity, and high substrate cost. To overcome these problems, an five enzymes cell-free cascade, consisting of alditol oxidase from Streptomyces coelicolor A3 (ScALDO), dehydratase from Paralcaligenes ureilyticus (PuDHT), tyrosine phenol lyase from Citrobacter freundii (CfTPL), tyrosine ammonia lyase from Rhodotorula glutinis (RgTAL) and phenylacrylate decarboxylase from Enterobacter sp. (EsPAD ) , was constructed for the de novo biosynthesis of p- hydroxystyrene from glycerol. Through process strategy optimization, a molar conversion yield of more than 84.2% was finally obtained from 10 mM crude glycerol. It is worth mentioning that the reaction system performed an excellent titer using 50 mM crude glycerol, and the p- hydroxystyrene concentration reached 30.32 mM, which exceeded the maximum titer of fermentation. This showed that our system is robust to p- hydroxystyrene and crude glycerol. In addition, a ‘two-step purification process’ was designed to produce pure p- hydroxystyrene. This is the first study focused on the cell-free biosynthesis of p- hydroxystyrene, and the highest conversion yield and titer thus far was obtained for p- hydroxystyrene de novo biosynthesis.
研究领域:
化学工程
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生物技术
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生物化学
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DOI
高影响因子
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
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2024
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IF 3
Zhou Jian-Feng,Xu Hai-Xia,Yin Zhong-Ping,Chen Ji-Guang,Zhang Qing-Feng
Quercetin is the most abundant flavonoid found in human diet. In the present study, the effects of quercetin on postprandial hyperglycemia (PPHG) were investigated in two ways. First, the complexes of quercetin and starch were prepared and characterized. Quercetin can occupy the helix of starch and interact with it through secondary bonds. The digestibility of starch decreased with the increasing proportion of quercetin in the complexes. Second, the inhibitory activities of quercetin on α-glucosidase and α-amylase were investigated in both in vitro and in vivo. Quercetin showed very strong inhibitory activity on α-glucosidase, while its inhibitory activity on α-amylase was far weaker than acarbose. Quercetin is the non-competitive inhibitor of α-glucosidase. Fluorescence titration showed that quercetin and α-glucosidase had one binding site and the binding constant lgKa was 3.70. After oral administration of quercetin–starch complexes in rats, the maximum level of postprandial blood glucose was reduced and delayed. Besides, quercetin also improved the PPHG after administration of sucrose and maltose in rats. Hence, it is deduced that the regulation of quercetin on PPHG is the result of its combination effects on starch and digestive enzymes. The complexes of quercetin and starch may be useful for the development of low-glycemic food.
研究领域:
营养学
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药理学
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食品科学
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DOI
高影响因子
TALANTA
·
2025
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IF 6.1
Linxue Zhen,Zhichun Bi,Xinyue Zhang,Peize Jin,Donghui Song,Hui Huang,Yongxin Li
A nanozyme sensor array was developed combining with machine learning algorithms for the effective identification and differentiation of multiple antibiotic classes and antibiotic individuals. The four channel sensor array was composed by four copper-based nanozymes with laccase-like activity. The significant structural differences between different classes of antibiotics (such as Aminoglycosides, Tetracyclines, β-lactams, Nitroimidazole, Sulfonamides, Quinolones) resulted in the different effect on the nanozymatic reactions through interact with copper、substrate and so on. In addition, the differences between antibiotics in the same class also made certain effect on the nanozymatic reactions. Based on the above affect and differences, we combined the machine learning algorithms to successfully realize the identification of six major classes and sixteen individuals of antibiotics simultaneously for the first time. The proposed sensing strategy realized the facile identification of major categories of antibiotics, which has good application prospects in the rapid antibiotic identification in environmental water bodies.
研究领域:
生物化学
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环境科学
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医学检测
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产品关联
DOI
PubMed
高影响因子
Sensing and Bio-Sensing Research
·
2025
·
IF 4.9
Lu Dang,Aoxue Zhang,Jinqing Li,Jin Cao,Xiangke Rong,Minyang Su,Baoji Du,Yazhong Bu
In recent years, a new method has been developed for the quantitative detection of alkaline phosphatase (ALP) based on the signal-off principle using oxidase or peroxidase nanozymes. However, the nanozymes used usually lose their activity under alkaline conditions, making it difficult to match the pH for ALP-catalyzed hydrolysis. Consequently, this detection method requires additional pH adjustment, which complicates the detection process and makes it prone to errors. In this study, we explored the enhancing effect of adenosine triphosphate (ATP) on the peroxidase (POD) activity of platinum nanoparticles formed with different protectants (Pt-CS NPs, Pt-PVP NPs, and Pt-BSA NPs) under neutral and alkaline conditions. It was found that ATP enhanced the POD activity of positively charged Pt-CS NPs the most, manifested as a significant increase in the maximum reaction rate (V max ). Based on the enhanced alkaline catalytic effect of ATP on Pt-CS NPs, we used Pt-CS/ATP as a new peroxidase nanozyme system. This system can catalyze at pH 8.5, enabling the detection of ALP without the need for pH adjustment. The research shows that the linear range of this detection system is 5–120 U/L, and the detection limit is 0.5 U/L. This study confirms that ATP can enhance the catalytic activity of platinum nanoparticles under neutral and alkaline conditions, providing a feasible solution for the direct detection of ALP under alkaline conditions using nanozymes.
研究领域:
生物化学
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医学检测
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纳米技术
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
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IF 5.256
Junning Wang,Mengyuan Bao,Tianxiang Wei,Zhaoyin Wang,Zhihui Dai
An iron-nickel bimetallic metal−organic framework (FeNi-MOF) with inner peroxidase-like activity as a support for enzyme immobilization was synthesized for the first time. And the GOx-mediated synthesis of mimic multienzyme system (GOx/FeNi-MOF) was obtained by a one-step biomimetic mineralization . FeNi-MOF played a dual role of a peroxidase mimic and a protective coating. Therefore, the obtained multienzyme system showed both peroxidase-like activity of FeNi-MOF and the biological activity of natural enzyme . FeNi-MOF acted as a peroxidase mimic, showing a higher affinity for hydrogen peroxide (H 2 O 2 ). The enhanced catalytic activity of the FeNi-MOF may result from synergistic effect between iron and nickel. In addition, the GOx/FeNi-MOF was selected as a proof of concept and succeeded in a one-step colorimetric detection of glucose by tandem catalysis. A small amount of the product was used for glucose detection, and the detection range was 0.3–35 mM, which indicated that our sensor can meet the clinical needs for diagnose diabetes.
研究领域:
生物化学
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材料科学
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医学诊断
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DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 9
·1-11
·
IF 6.408
Sun Wenying,Wang Nan,Zhou Xiaobin,Sheng Yuxuan,Su Xingguang
A Co, N co-doped porous carbon-based nanozyme (Co–N-C nanozyme) has been fabricated. Taking advantages of the excellent oxidase catalytic activity and significant stability of Co–N-C nanozyme, we propose a fluorescence and colorimetric system based on Co–N-C nanozyme and red-emitting carbon quantum dots (RCDs) for butyrylcholinesterase (BChE) sensing. As the chromogenic substrate 3,3′,5,5′-tetramethylbenzidine (TMB) was catalyzed and oxidized by Co–N-C nanozyme, the generated oxTMB had a new absorption peak at 652 nm, which resulted in the significant quenching of the fluorescence of the carbon quantum dots at 610 nm. Under the catalysis of BChE, thiocholine was generated from the hydrolysis of S-butyrylthiocholine iodide (BTCh), and the as-generated thiocholine effectively inhibited the oxidation of TMB catalyzed by Co–N-C nanozyme, leading to a decrease of the absorption of oxTMB at 652 nm and effective fluorescence recovery of RCDs. By measuring the absorbance of produced oxTMB at 652 nm and the fluorescence of RCDs at 610 nm, the fluorescence and colorimetric system both exhibited an outstanding linear response to the activity of BChE in the range 0.5 to 40 U L −1 , with a detection limit of 0.16 U L −1 and 0.21 U L −1 , respectively. Furthermore, this established dual-channel biosensing strategy has been successfully applied to the determination of BChE in human serum samples. The present work has effectively expanded the development and application of nanozyme in biosensing. Graphical abstract
研究领域:
生物化学
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纳米生物学
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分析化学
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DOI
PubMed
高影响因子
ANALYST
·
2020
·Vol. 146
·期 3
·896-903
·
IF 3.978
Xiaolei Xie,Yufei Wang,Xiaobin Zhou,Junyang Chen,Mengke Wang,Xingguang Su
Single-atom nanozymes have drawn wide attention in bio-sensing for their remarkable merits such as low cost, high stability, and maximum atom utilization. Herein, a colorimetric strategy based on Fe–N–C single-atom nanozymes (Fe/NC-SAs) was established for the detection of alkaline phosphatase (ALP) activity. The Fe/NC-SAs prepared by pyrolysis have excellent peroxidase-like activity and can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to a blue color product in the presence of hydrogen peroxide (H2O2). When ascorbic acid (AA) is added to the system, the blue color fades, and the absorbance has a linear relationship with the concentration of AA. Alkaline phosphatase (ALP) can catalyze the hydrolysis of ascorbic acid 2-phosphate (AAP) to produce AA. Thus, a strategy based on Fe/NC-SAs for the detection of ALP activity was established, which provided a linear range of 0.1–1.5 U L−1 and a limit of detection as low as 0.05 U L−1. Besides, Fe/NC-SAs showed high stability under harsh conditions. Moreover, an Fe/NC-SA-based assay was successfully validated using human serum samples for ALP determination with satisfactory results, and has broad prospects in the field of biosensing.
研究领域:
生物传感
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医学检测
·
纳米材料
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Xin Xu,Peng Luo,Huan Yang,Shuang Pan,Hui Liu,Xiaoli Hu
This paper reports a facile approach for fabricating metal–adenosine triphosphate (ATP) nanoparticles (NPs) by the self-assembly of ATP and metal ions under moderate conditions. Here, Cu-ATP NPs only showed laccase-like activity, but the Fe 3+ -doped Cu-ATP NPs could exhibit peroxidase-like activity, resulting in a decrease in laccase-like activity. Mn 2+ ions functioned as a regulator to balance the enzyme-like activities of CuFe-ATP NPs, endowing the CuMnFe-ATP NPs with both strong laccase- and peroxidase-like activities. Surprisingly, the CuMnFe-ATP NPs also exhibited catalase-like activity under neutral conditions, improving the efficiency of phenol oxidation. X-ray photoelectron spectroscopy (XPS) analysis confirmed that the mixed valence states of the metals in the CuMnFe-ATP NPs accounted for the high catalytic efficiency; also, the large surface area and intrinsic porosity of the NPs were of great importance. The kinetic studies results indicated that the CuMnFe-ATP NPs exhibited rather strong affinities to 3,3,5′,5′-tetramethylbenzidine (TMB) and 2, 4-dichlorophenol (2,4-DP). On the basis of peroxidase-mimic activity, the CuMnFe-ATP NP system was employed to establish a facile colorimetric biosensing method for H 2 O 2 determination, and the detection limit of H 2 O 2 was 0.047 μmol⋅L −1 . These results prove that the enzyme-like activities of Cu-ATP NPs can be modulated via simple metal doping, which may extend to other nanozymes.
研究领域:
生物化学
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纳米生物学
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分析化学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）