TALANTA
·
2025
·
IF 6.1
Terefe Tafese Bezuneh,Fuchun Nan,Xin Li,Tongtong Kou,Zilong Wu,Fangfang Lang,William W. Yu
Designing highly sensitive method for the analysis of α-glucosidase activity is of critical value for diagnosis and therapy of diabetes. Here, we employed self-assembly of guanosine 5′-monophosphate nucleotide (GMP) with metal ions (Cu 2+ and Tb 3+ ) to prepare multifunctional Tb-CuGMP NPs. Owing to Cu 2+ -catalytic center, the as-prepared Tb-CuGMP NPs exhibits ascorbic acid oxidase (AAO)-like and peroxidase (POD)-like self-cascading catalytic activity. The AAO-POD cascade system can catalyze the oxidation of AA into dehydroascorbic acid (DHAA) with the generation of H 2 O 2 , which serves as a substrate for POD-like activity and catalyzed into • OH radicals. Based on these enzymatic reactions, a unique colorimetric detection approach in which nanozyme/TMB/AA produced oxidized TMB with characteristic blue color and DHAA sensitized Tb 3+ to turn-on the fluorescence of the detection system was proposed. Furthermore, enzymatic hydrolysis of 2-O-a-D-glucopyranosyl-L-ascorbic acid (AA2G) into AA was coupled with AAO-POD cascade system to realize highly sensitive detection of α-glucosidase activities. Interestingly, the as-constructed dual-mode assay reduces the LOD of α-glucosidase to 5.1 × 10 −4 U/mL. To sum up, this work provides new horizon for the development of efficient multifunctional nanomaterial with integration of biocatalysis and bioassay into a single material.
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BMC PLANT BIOLOGY
·
2025
·Vol. 25
·期 1
·1-17
·
IF 4.8
Deng Dehai,Qin Yichao,Lin Xiuying,Chu Meng,Lv Daizhu,Lin Huan
Background The oil palm ( Elaeis guineensis ) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops. Results Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2’-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids. Conclusions This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.
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BMC MICROBIOLOGY
·
2025
·Vol. 25
·期 1
·1-13
·
IF 4.2
Cao Xiaoyu,Zhang Yu,Xu Qianqian,Yan Hai
The overproduction of uric acid, driven by its key precursors (inosine and guanosine), leads to hyperuricemia, a metabolic disorder associated with severe complications such as gout and renal dysfunction. Here, a promising bacterial strain YC06 with excellent biodegradation capability for inosine and guanosine was successfully isolated from healthy individual feces and identified as Bacillus paranthracis through average nucleotide identity (ANI) analysis. B. paranthracis YC06 resting cells (live but suspended in PBS buffer) and its cell-free extracts could effectively biodegrade inosine and guanosine in vitro. Whole-genome sequencing revealed a 5,535,183 bp draft genome (52 contigs, 35.22% GC content) containing 5,672 protein-coding genes. B. paranthracis YC06 demonstrated high survival rates in simulated gastrointestinal fluids, supported by the presence of stress-response genes and bile salt hydrolase genes associated with gastrointestinal tolerance. However, this strain exhibited hemolytic activity and no amino acid decarboxylase activity, while hemolysin genes, antibiotic genes and toxin-producing genes were identified, raising potential biosafety concerns for its further application. The gene functional annotation and polymerase chain reaction (PCR) amplification electrophoresis identified key genes ( rihA , rihB , deoD and pnp ) encoding purine nucleosidase and purine-nucleoside phosphorylase, and combined with biodegradation product analysis, elucidated the pathways of inosine and guanosine biodegradation into hypoxanthine and guanine. Comprehensive safety evaluations, including cytotoxicity assay and in vivo pathogenicity studies, must be conducted to rigorously assess its risks prior to practical utilization.
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Probiotics and Antimicrobial Proteins
·
2025
·
IF 4.4
Huang Fang,Lu Yun-hui,Xiong Jie,Zeng Xin-an,Huang Yan-yan,Wang Lang-hong
The escalating global burden of hyperuricemia, driven by modern dietary patterns and sedentary lifestyles, has prompted urgent exploration of novel therapeutic strategies, with particular emphasis on developing targeted probiotic interventions as a potential alternative to conventional pharmacological approaches. This study successfully isolated Limosilactobacillus fermentum FOSU YHD19 from fermented foods, demonstrating remarkable in vitro uric acid reduction of 12.90% along with high-level degradation of nucleosides. A comprehensive safety evaluation confirmed the absence of harmful enzymatic activities (including nitroreductase, decarboxylase and tryptophanase), hemolytic properties, or antibiotic resistance. The strain exhibited strong stress tolerance, surviving in acidic conditions (pH 2.0), with exposure to gastric juice, and when in the presence of 0.1% bile salts. Additionally, YHD19 demonstrated potent antibacterial activity against multiple pathogenic strains, including E coli , S. aureus , L. monocytogenes , and S. enteritidis . Genomic analysis revealed three nucleoside hydrolases and six transporter systems that collectively facilitate the hypouricemic activity. By ruling out the presence of key uric acid-synthesizing enzymes (xanthine dehydrogenase/oxidase and guanine deaminase), we can attribute the hypouricemic effect primarily to a nucleoside-scavenging mechanism. In addition, the genome harbors 91 stress-responsive genes implicated in acid/base adaptation, thermal shock response, and oxidative defense mechanisms, underscoring the strain’s resilience under gastrointestinal stresses. These findings position YHD19 as a safe and effective probiotic candidate for functional foods aimed at managing hyperuricemia, supporting its safety profile for potential probiotic applications.
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Analytical Methods
·
2025
·
IF 2.6
Chao-Zhe Zhu,Shuo Tian,Hua-Nong Ting,Mengke Wang,Yufeng Wang
The portable, rapid, and accurate detection methods for alkaline phosphatase (ALP) can significantly improve the efficiency and accuracy of medical testing, especially in early disease screening. Herein, a fluorescent detection was constructed for ALP based on the ALP-mediated in situ-generated fluorescent product for the first time, which employed the Au nanoclusters (AuNCs) and ALP-mediated in situ generated fluorescent products as probes by applying the 4-methylumbelliferyl phosphate (MeUP) as a substrate. ALP can catalyze the hydrolysis of MeUP to generate 4-methylumbelliferon (MeU), which exhibits blue emission centered at 440 nm. Meanwhile, the AuNCs kept the red fluorescence of AuNCs at 630 nm as a reference signal. Accordingly, a ratio fluorescent sensing of ALP was proposed with a consecutive fluorescent color change with a detection limit of 0.0025 U/L. The pronounced fluorescence signal variation served as the impetus for the development of a facile, cost-effective portable hydrogel kit, which facilitates equipment-free, visual, and quantitative analysis of ALP activity. Additionally, this system was employed for detecting ALP inhibitors using sodium metavanadate (Na3VO4) as a model. Notably, this sensing method and hydrogel kit were applied for ALP detection in human serum samples, yielding promising results.
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Redox Biology
·
2017
·
IF 6.337
Dan-Qian Chen,Gang Cao,Hua Chen,Dan Liu,Wei Su,Xiao-Yong Yu,Nosratola D. Vaziri,Xiu-Hua Liu,Xu Bai,Li Zhang,Ying-Yong Zhao
Changes in plasma concentration of small organic metabolites could be due to their altered production or urinary excretion and changes in their urine concentration may be due to the changes in their filtered load, tubular reabsorption, and/or altered urine volume. Therefore, these factors should be considered in interpretation of the changes observed in plasma or urine of the target metabolite(s). Fasting plasma and urine samples from 180 CKD patients and 120 age-matched healthy controls were determined by UPLC-HDMS-metabolomics and quantitative real-time RT-PCR techniques. Compared with healthy controls, patients with CKD showed activation of NF-κB and up-regulation of pro-inflammatory and pro-oxidant mRNA and protein expression as well as down-regulation of Nrf2-associated anti-oxidant gene mRNA and protein expression, accompanied by activated canonical Wnt/β-catenin signaling. 124 plasma and 128 urine metabolites were identified and 40 metabolites were significantly altered in both plasma and urine. Plasma concentration and urine excretion of 25 metabolites were distinctly different between CKD and controls. They were related to amino acid, methylamine, purine and lipid metabolisms. Logistic regression identified four plasma and five urine metabolites. Parts of them were good correlated with eGFR or serum creatinine. 5-Methoxytryptophan and homocystine and citrulline were good correlated with both eGFR and creatinine. Clinical factors were incorporated to establish predictive models. The enhanced metabolite model showed 5-methoxytryptophan, homocystine and citrulline have satisfactory accuracy, sensitivity and specificity for predictive CKD. The dysregulation of CKD was related to amino acid, methylamine, purine and lipid metabolisms. 5-methoxytryptophan, homocystine and citrulline could be considered as additional GFR-associated biomarker candidates and for indicating advanced renal injury. CKD caused dysregulation of the plasma and urine metabolome, activation of inflammatory/oxidative pathway and Wnt/β-catenin signaling and suppression of antioxidant pathway.
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JOURNAL OF SEPARATION SCIENCE
·
2015
·Vol. 38
·期 7
·1082-1089
·
IF 2.737
Liyun Ma,Simin Liu,Qing Wang,Lin Yao,Li Xu
In the present study, one of the new generation of host molecules, cucurbit(6)uril (CB(6)), was immobilized onto silica (CB(6)/SiO 2 ) by a sol–gel approach. CB(6)/SiO 2 was characterized by NMR spectroscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and elemental analysis. It was used as a high-performance liquid chromatographic stationary phase and its chromatographic performance was systematically investigated with different types of analytes as probes. The results revealed that the CB(6)/SiO 2 stationary phase exhibited weak hydrophobic and strong hydrophilic properties. Hence, the variables for hydrophilic interaction liquid chromatography, including components and pH of the mobile phase, were further investigated to explore the retention mechanism of this CB(6)/SiO 2 stationary phase. For less polar analytes, both hydrophobic and hydrophilic interactions could contribute to the retention, while for polar analytes, hydrophilic interaction may be predominant. Compared to the tetraethoxylsilane-coated SiO 2 stationary phases, the CB(6)/SiO 2 stationary phase exhibited a different retention behavior toward basic analytes with excellent stability. It is a novel promising hydrophilic interaction liquid chromatography stationary phase.
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ANALYTICAL SCIENCES
·
2018
·Vol. 34
·期 4
·433-438
·
IF 1.355
Li Hengye,Zhang Xuemeng,Zhang Lin,Cang Hui,Kong Fenying,Fan Dahe,Wang Wei
Surface-initiated anionic-ring-opening multibranching polymerization was employed to prepare a hyperbranched polyglycerol (HPG) functionalized silica stationary phase for hydrophilic interaction liquid chromatography (HILIC). The obtained stationary phase was characterized by Fourier-transform infrared spectrometry (FT-IR) and thermogravimetric analysis (TGA). The chromatographic properties of the prepared stationary phase were systematically investigated. The abundance and multitude distribution of hydroxyl groups in HPG endowed the stationary phase with improved hydrophilicity and enhanced separation performance compared with the stationary phase functionalized with monolayer of hydroxyl groups. The stationary phase showed excellent retention of various polar compounds, such as nucleosides, necleobases, phenols and sulfanilamides, indicating great potential in the separation of complex biosamples.
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ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Qing Wang,Mao Ye,Li Xu,Zhi-guo Shi
A mixed-mode chromatographic packing material, C18 and diol groups modified silica (C18-Diol), was prepared with controllable hydrophobicity and hydrophilicity . It demonstrated excellent aqueous compatibility and stability in aqueous mobile phase ; compared to the traditional C18 column, improved peak shape of basic analytes was also obtained. Additionally, it exhibited both reversed-phase liquid chromatographic (RPLC) and hydrophilic interaction chromatographic (HILIC) performance; the analyte separation scope was thus enlarged, demonstrated by simultaneous separation of twenty acids, bases and neutrals. More interestingly, a novel on-line two-dimensional liquid chromatography on the single column (2D-LC-1C) was established by modifying the high performance liquid chromatographic instrument only with the addition of an extra six-port two-position valve. The early co-eluted components of the extract of Lonicera japonica on the 1st-dimension (RPLC) were collected for the online re-injection to the 2nd-dimension (HILIC) by conveniently varying the mobile phase components. Six more peaks were obtained. The established system was simple, easy operation and low cost, which had advantages in analyzing complicated samples.
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Food Bioscience
·
2025
·
IF 5.9
Zixi Li,Meng Dong,Libo Qi,Shan Shang,Yan Guo,Lei Qin,Baoshang Fu
Frying is the necessary step of fish soup production, which directly affects the flavor and nutritional quality of the soup. To investigate the effect of edible oil on the final quality of fish bone soup, five kinds of oil were employed in this study. The results showed that olive oil could promote the dissolution of glutamic acid and aspartic acid into the soup, the umami value elevated nearly four times. Meanwhile, olive oil and sunflower oil brought more volatile flavor substances, which contributed to the highly accepted flavor. Triglyceride O-16:0/18:1/18:2 (palmitic/oleic/linoleic triglycerides) was regarded as a potential precursor for the formation of flavor substances, such as 1-octen-3-one and 2-heptanone, which contribute to the specific flavor of fish bone soup. This study revealed that frying would improve the quality of fish bone soup by changing the color and flavor profile, guiding the selection oil for the industrial processing of fish bone soup.
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GEOCHIMICA ET COSMOCHIMICA ACTA
·
2025
·
IF 5
Yinyin Qian,Beibei Shi,Guangyao Li,Huaming Yang
Kaolinite nanoclay has long been recognized as playing a key role in the origin of life. However, the interaction of kaolinite (Kaol) with biomolecules in prebiotic chemistry and its catalytic mechanism still remain a great challenge. Herein, we comprehensively investigate the nucleoside interactions and phosphorylation processes on Kaol surfaces by a combination of wet-dry cycle experiments and first-principles calculations. The experimental results show that Kaol is able to promote the phosphorylation reactions of four typical nucleosides, but there are differences in the catalytic ability for different nucleosides. In this study, we considered two physicochemically different Kaol end-surfaces—the aluminium-hydroxyl surface (Kaol(001)) and the silica-oxygen surface (Kaol(00 1 ¯ ))—and find that Kaol(001) forms strong hydrogen bonds with the nucleoside, stabilizing the nucleoside that significantly reduces the kinetic barrier to phosphorylation. Moreover, ab initio molecular dynamics (AIMD) simulations showed that the Kaol(001)-nucleotide system is kinetically stable, highlighting its potential in protecting the reaction sites of prebiotic molecules and providing strong evidence for the role of Kaol in prebiotic chemistry.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Rikun Cui,Linna Li,Lili Liu,Zhen Li,Zhenzhao Wang,Xingtong Liu,Lei Tian,Fujun Yao,Xiaofeng Kang,Yanli Guo
Nucleic acid aptamers, which are short single-stranded RNA or DNA molecules, undergo conformational changes upon recognizing small molecules. However, their structural characterization is challenging due to the flexibility of single-stranded oligonucleotides. Nanopores provide a sensitive method for single-molecule analysis of molecular conformational changes as they transport through the pore. In this study, we employed α-hemolysin (α-HL) nanopore with high spatiotemporal resolution to investigate the conformational changes of DNA aptamer (ADO-23) upon binding to adenosine (Ado) molecules. Unlike the events generated by the aptamer alone, the DNA-Ado complex produced two distinct new characteristic events, indicating the formation of two unique secondary structures. By analyzing current events, the structures formed by the DNA-Ado complex were speculated to be hairpin DNA and G-quadruplex (G4) DNA. Furthermore, we demonstrated the potential of the aptamer as a chiral selector within the nanopore. The results showed that the aptamer specifically bound to D-Ado and not to L-Ado. Finally, we realized specific and sensitive detection of small molecule Ado by α-HL pore, with a detection limit as low as 10.5 nM, and successfully applied it to the detection of real human serum samples. The nanopore platform provides a powerful tool for studying small molecule-biomolecule conformational changes, and we constructed sensors for detecting adenosine through the aptamer. This work also offers a promising new approach to the recognition of enantiomers at the single-molecule level.
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