Neural Regeneration Research
·
2022
·Vol. 18
·期 8
·1763
·
IF 6.058
Yang Li-Xiang,Yao Yuan-Yuan,Yang Jiu-Rong,Cheng Hui-Lin,Zhu Xin-Jian,Zhang Zhi-Jun
Destruction of the blood-brain barrier is a critical component of epilepsy pathology. Several studies have demonstrated that sphingosine 1-phosphate receptor 1 contributes to the modulation of vascular integrity. However, its effect on blood-brain barrier permeability in epileptic mice remains unclear. In this study, we prepared pilocarpine -induced status epilepticus models and pentylenetetrazol -induced epilepsy models in C57BL/6 mice. S1P1 expression was increased in the hippocampus after status epilepticus, whereas tight junction protein expression was decreased in epileptic mice compared with controls. Intraperitoneal injection of SEW2871, a specific agonist of sphingosine-1-phosphate receptor 1, decreased the level of tight junction protein in the hippocampus of epileptic mice, increased blood-brain barrier leakage, and aggravated the severity of seizures compared with the control. W146, a specific antagonist of sphingosine-1-phosphate receptor 1, increased the level of tight junction protein, attenuated blood-brain barrier disruption, and reduced seizure severity compared with the control. Furthermore, sphingosine 1-phosphate receptor 1 promoted the generation of interleukin-1β and tumor necrosis factor-α and caused astrocytosis. Disruption of tight junction protein and blood-brain barrier integrity by sphingosine 1-phosphate receptor 1 was reversed by minocycline, a neuroinflammation inhibitor. Behavioral tests revealed that sphingosine 1-phosphate receptor 1 exacerbated epilepsy -associated depression-like behaviors. Additionally, specific knockdown of astrocytic S1P1 inhibited neuroinflammatory responses and attenuated blood-brain barrier leakage, seizure severity, and epilepsy -associated depression-like behaviors. Taken together, our results suggest that astrocytic sphingosine 1-phosphate receptor 1 exacerbates blood-brain barrier disruption in the epileptic brain by promoting neuroinflammation .
研究领域:
神经科学
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药理学
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病理生理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
·
2016
·
IF 3.905
Longguang Jiang,Lin Lin,Rui Li,Cai Yuan,Mingming Xu,Joy H. Huang,Mingdong Huang
Platelet endothelial cell adhesion molecule 1 (PECAM-1) is a cell surface receptor widely distributed on endothelium and hematopoietic-derived cells, and maintain the integrity of the blood vessels. PECAM-1 is widely recognized as an endothelial cell marker. The homophilic interaction through its extracellular domain of PECAM-1 (soluble PECAM-1, or sPECAM-1) is important to its functions. However, structural details for such dimerization are not fully understood. Here we report the production of recombinant sPECAM-1 in large quantity by Drosophila expression system and the small-angle X-ray diffraction (SAXS) study. The recombinant sPECAM-1 was found to form one population of dimer, but not oligomer, and was able to bind to heparin immobilized on a chip in surface plasmon resonance imaging (SPRi) binding experiments. The results of SAXS demonstrated that sPECAM-1 formed a symmetric homodimer of Ω-shape in solution, and each protomer adopted an extended conformation. The dimer is mediated through the intermolecular interactions through the first N-terminal domain (D1) of sPECAM-1. This model provides new structural information for its homophilic interaction mechanism.
研究领域:
分子生物学
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结构生物学
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免疫学
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血管生物学
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生物化学
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转化医学
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DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2017
·
IF 2.323
Tiantian Ye,Haibin Qu,Xingchu Gong
The liquid–liquid equilibrium (LLE) data of ternary systems of water + d-fructose + 1-butanol, water + d-glucose + 1-butanol, and water + d-galactose + 1-butanol were determined at three temperatures (288.2, 303.2, and 318.2 K) and atmospheric condition. The distribution coefficients of the monosaccharides were lower than 0.05. For all the three saccharides, their content in the organic-rich phase increased as their content in the aqueous phase increased. The experimental tie-line data were fitted using the Othmer–Tobias equation. The saccharide universal quasichemical functional-group activity coefficient (S-UNIFAC) model was used to predict the LLE data with all the average absolute deviations (AAD) values higher than 0.013. The modified universal quasichemical model was then used to correlate the LLE data. The correlation results agreed better with experimental data than the prediction results obtained with S-UNIFAC. In the production of traditional Chinese medicines, 1-butanol is a promising extractant used to separate active compounds and saccharides in aqueous herbal extracts.
研究领域:
化学工程
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生物化学
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DOI
高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2016
·Vol. 121
·期 6
·1616-1626
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IF 2.156
Huang X.,Shi J.,Cui C.,Yin H.,Zhang R.,Ma X.,Zhang X.
AimsWe aimed to investigate the phenanthrene degradation characteristics and possible phenanthrene degradation pathways of Rhizobium petrolearium SL‐1.Methods and ResultsUsing high‐performance liquid chromatography (HPLC), UV‐visible and gas chromatography‐mass spectrometry (GC‐MS) analysis, the phenanthrene‐degrading properties and metabolites of Rh. petrolearium SL‐1 were analysed, and then combined with genome‐based analysis to elucidate the possible biodegradation pathway of phenanthrene in Rh. petrolearium SL‐1. The results of the analyses showed that phenanthrene (100 mg l−1) was completely degraded by strain SL‐1 at 35°C, 0·02% salinity and pH 9·0 within 3 days. A wide range of polycyclic aromatic hydrocarbons, including naphthalene, fluorene, anthracene and pyrene, could also be degraded by this strain.ConclusionsBased on the identified metabolites, the utilization of probable intermediates and the presence of putative phenanthrene catabolic genes, we concluded that phenanthrene was degraded via two different routes, namely the ‘naphthalene’ and the ‘phthalic acid’ routes.Significance and Impact of the StudyFor the first time, this study shows the degradation pathway of phenanthrene by a Rhizobium strain. Because of its excellent stress resistance, metabolic versatility, high degradation efficiency and potential application in phytoremediation, Rh. petrolearium SL‐1 is a potential candidate for the bioremediation of polycyclic aromatic hydrocarbons‐contaminated areas.
研究领域:
环境微生物学
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分子生物学
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生态学
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DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2024
·Vol. 87
·期 6
·407-418
·
IF 1.2
Hong Lei,Mao Huihui,Huang Xiaowei,Fu Xinyang,Lin Yanfang
To analyze 1-methyladenosine (m1A) and 1-methylinosine (m1I) in urine samples obtained from patients with breast cancer and healthy people, we used magnetic dispersive solid-phase micro-extraction (MDSPME) combined with high-performance liquid chromatography. The structure and morphology of magnetic nanoparticles, Fe 3 O 4 @GO, were investigated by FT-IR, XRD, TEM, and TGA. m1A and m1I in urine samples were enriched by the solid phase micro-extraction technology of Fe 3 O 4 @GO. Several parameters affecting the extraction efficiency, including adsorption time, pH, ionic strength, mass of adsorbent, extraction time, eluent solvent, elution time, and elution temperature, were researched and optimized. Under the optimized conditions, the corresponding linearity range of the two analytes showed a good linearity ( r 2 > 0.9998) and significant addition recoveries ranging from 88.50 to 109.38%, while the limit of detection was between 0.10 and 0.11 ng/mL. The enrichment factors of m1A and m1I were 108 and 134, respectively. The method was simple, sensitive, and reliable for the detection and purification with good detection limit, and it was suitable for the detection of m1A and m1I in urine samples. In addition, the analysis of modified nucleosides is of great value for the early diagnosis of patients with breast cancer.
研究领域:
分析化学
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肿瘤学
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医学诊断
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DOI
高影响因子
Molecular Medicine Reports
·
2020
·Vol. 21
·期 6
·2466-2474
·
IF 2.1
Lu Wang,Liran Zhu,Chenfangyuan Duan,Lu Li,Guangliang Chen
Total saponins extracted from Dioscorea collettii (TSD), extracts of the Chinese herb Dioscorea, are thought to exhibit therapeutic benefit in gouty arthritis. However, its exact mechanism remains unclear. The current study aimed to elucidate the underlying mechanisms by investigating the effects of TSD on the inflammation induced by monosodium urate (MSU) crystals in THP‑1 macrophages. The viability of THP‑1 macrophages was examined using the MTT assay and the levels of inflammatory cytokines, including interleukin (IL)‑1β, IL‑18 and tumor necrosis factor (TNF)‑α, released by the cells were quantitatively measured using ELISA kits. The results revealed that the protein level of cluster of differentiation 11b increased in THP‑1 cells treated with 100 ng/ml phorbol ester, suggesting that monocytic THP‑1 cells were successfully differentiated into macrophages. TSD decreased the levels of inflammatory cytokines, including TNF‑α, IL‑18 and IL‑1β, secreted by THP‑1 macrophages. As the release of IL‑1β and IL‑18 is dependent on the NLR family pyrin domain containing 3 (NALP3) inflammasome and caspase‑1, the current study investigated the effect of TSD on the aforementioned proteins. The results revealed that TSD decreased the protein levels of NALP3 and apoptosis‑associated speck‑like, which serve important roles in the assembly of the NALP3 inflammasome. Furthermore, NALP3 inflammasome‑related proteins were also decreased by TSD in rotenone induced THP‑1 macrophages, TSD inhibited the activation of caspase‑1 and rotenone‑induced NALP3 inflammasome activation in THP‑1 macrophages. The results obtained in the current study revealed that TSD attenuated MSU crystal‑induced inflammation by inhibiting rotenone‑induced activation of the NALP3 inflammasome and caspase‑1, suggesting that these two proteins may be novel targets for the treatment of gouty arthritis.
研究领域:
药理学
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免疫学
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炎症研究
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中医药
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产品关联
DOI
PubMed
高影响因子
MICROPOROUS AND MESOPOROUS MATERIALS
·
2018
·
IF 3.649
Qi Du,Yiping Guo,Pei Wu,Hezhou Liu
Hierarchically porous TS-1 (HTS-1) zeolite with nanocrystal aggregate structure has been successfully synthesized by hydrothermal and steam-assisted crystallization (SAC) method with the aid of polyvinyl alcohol (PVA). It has been found that the formation of mesoporous aggregates was attributed to the construction of PVA three-dimensional network. The mesoporous aggregate structure could be well maintained by SAC method, resulting in HTS-1 with either surface titanium enrichment or highly interconnected mesopores and high external surface. Both could contribute to the excellent catalytic performance of HTS-1 in oxidative desulfurization of dibenzothiophene (DBT) and 4,6-dimethyldibenzothiophene (4,6-DMDBT). The TOF numbers of HTS-1 reached a very high value to 114.9 h −1 and 53.2 h −1 in the oxidation of DBT and 4,6-DMDBT, respectively. Moreover, the HTS-1 showed good stability and remained ∼90% of its initial activity even after 3 times cycle. The high catalytic activity and good stability suggest that HTS-1 is a promising catalyst for deep oxidative desulfurization.
研究领域:
催化化学
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材料科学
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环境化学
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DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Fang-Fang Liu,Hai-Yan Ren,Feng Liu,Wen-Xuan Qi,Chao-Peng Liang,Bang-Xian Zhang,Xiang-Jun Rao
Insects mount robust immune responses to reduce the efficacy of biopesticides. Identification of critical immune targets for biocontrol strategies is thus essential. However, immune targets in the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), a major pest of corn, remain largely unexplored. Here, we investigated the immune response of S. frugiperda to the entomopathogen Beauveria bassiana (Hypocreales: Cordycipitaceae). Time-series RNA sequencing revealed 863, 717, and 165 differentially expressed genes (DEGs) at 24, 48, and 60 h post-infection, respectively. GO and KEGG enrichment analyses revealed that DEGs were significantly enriched in immune-related terms and pathways. Among the DEGs, the gene encoding immulectin SfIML-1 , a C-type lectin with dual carbohydrate recognition domains, was significantly upregulated during the early and intermediate stages of infection. Genome-wide analysis identified nine IML genes, with SfIML-1 as the only one highly responsive to B. bassiana . Recombinant SfIML-1 binds pathogen-associated molecular patterns and pathogens, enhancing cellular responses. RNAi of SfIML-1 decreased survival after fungal infection, while injection of recombinant SfIML-1 increased survival. Suppression of the NF-κB and JAK-STAT pathways diminished SfIML-1 expression in both fat body and Sf9 cells. These results identify SfIML-1 as a key pattern recognition receptor and a promising target for developing novel biocontrol strategies.
研究领域:
昆虫学
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分子生物学
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生物防治
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DOI
PubMed
高影响因子
ANALYTICAL LETTERS
·
2023
·
IF 2
Jiajia Wang,Xiaomin Yang,Xin Hua,Yanan Li,Baokang Jin
Previous studies confirm that aberrant expression of cytokeratin 19 fragment antigen 21-1 (cyfra-21-1) is highly correlated with non-small cell lung cancer (NSCLC). Herein, a proportional electrochemical immunosensor based upon a methylene blue/cadmium telluride/molybdic sulfide (MB/CdTe/MoS2) nanocomposite was successfully fabricated for detecting tumor marker cyfra-21-1. The result indicates that MB/CdTe/MoS2 modified on the surface of a glassy carbon electrode (GCE) supplies a large surface area for immobilizing primary antibodies (Ab1), ensuring high selectivity and sensitivity. To further amplify the sensitivity of the biosensor, ferrocenecarboxylic acid (Fc-COOH) linked with amino-functionalized secondary antibodies through amido bond (Ab2-Fc) as the “signal-on” probe and MB-modified with MoS2 with a large specific surface area as the “signal-off” probe increase the number of active sites for integration of Ab2-Fc/Ag/BSA/Ab1/MB/CdTe/MoS2/GCE electrode with the linker cyfra-21-1 (Ag). Meanwhile, CdTe accelerates the electron transfer and enhances the MB current, improving the sensitivity and detection limit. The presence of cyfra-21-1 led to both the decreasing response of MB and increasing current responses of Fc upon the electrode. Based upon this strategy, the determination of cyfra-21-1 was achieved in a linear range from 10 pg/mL to 10,000 ng/mL with the lowest detectable concentration of 10 pg/mL (signal-to-noise ratio of 3). The electrochemical immunosensor performs a sensitive determination of cyfra-21-1, possibly providing a practical strategy for the clinical analysis of lung cancer.
研究领域:
生物化学
·
分析化学
·
肿瘤学
·
纳米技术
·
电化学
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产品关联
DOI
高影响因子
Materials
·
2018
·Vol. 11
·期 5
·791
·
IF 2.467
Junping Ding,Huanchun Wang,Haomin Xu,Lina Qiao,Yidong Luo,Yuanhua Lin,Cewen Nan
High efficiency photocatalyst Bi2O2(CO3)1−xSxwas synthesized conveniently with chemical bath precipitation using Bi2O2CO3as the precursor. The microstructures of the samples are systematically characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM), X-ray photoelectron spectroscopy (XPS), ultraviolet photoelectron spectroscopy (UPS) and UV-Vis spectroscopy; the optical and photocatalytic properties are carefully tested as well. The content ofS, which was tuned through the controlling of the precipitation process, was verified to have an intense effect over the photocatalytic properties. A nearly saturatedSratio and the best photocatalytic performance were observed in specimens with the mostScontent. Our study reveals that, with negligible influence of the morphology and crystal structure, Bi2O2(CO3)1−xSxpossessed a broadened optical absorption regionfromultraviolet to visible light, and enhanced photocatalytic activity in comparison to precursor Bi2O2CO3in photocatalytic degradation of Congo Red aqueous solution.Keywords:Bi2O2CO3;Bi2O2(CO3)1−xSx;broadband spectra;photocatalysis
研究领域:
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Molecular Medicine Reports
·
2018
·Vol. 18
·期 2
·1789-1797
·
IF 1.922
Jian‑Xia Shi,Qin Huang
Glucagon‑like peptide‑1 (GLP‑1) is a gut incretin hormone that is considered to be a promising target for the treatment of patients with type 2 diabetes. However, the mechanisms underlying the protective effects of GLP‑1 on diabetic nephropathy are yet to be fully elucidated. Sirtuin (SIRT)1 encodes a member of the SIRT family of proteins that serves an important role in mitochondrial function and is reported to be associated with the pathogenesis of chronic kidney disease. The present study treated mouse podocytes with various concentrations of D‑glucose to establish a high glucose (HG)‑induced model of renal injury. The results of a 2',7'‑dichlorodihydrofluorescein diacetate assay, Annexin V/propidium iodide staining and ELISA demonstrated that treatment of podocytes with HG significantly enhanced the production of reactive oxygen species (ROS), promoted cell apoptosis and increased the secretion of proinflammatory cytokines, respectively. The cytokines increased following HG treatment included tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6. Notably, treatment with GLP‑1 attenuated HG‑induced increases in ROS production and podocyte apoptosis, which may occur via downregulation of the expression of caspase‑3 and caspase‑9, and increased expression of nephrin, podocin and SIRT1, as determined by reverse transcription‑quantitative polymerase chain reaction and western blot analysis. Treatment with GLP‑1 led to protective effects in podocytes that were similar to those of resveratrol. Furthermore, SIRT1 knockdown using short hairpin RNA significantly enhanced the expression of caspase‑3 and caspase‑9 in mouse podocytes, compared with normal mouse podocytes. SIRT1 knockdown with or without GLP‑1 administration significantly decreased the expression of caspase‑3 and caspase‑9 in mouse podocytes, compared with SIRT1 knockdown mouse podocytes. In conclusion, the results of the present study indicated that GLP‑1 may be a promising target for the development of novel therapeutic strategies for HG‑induced nephropathy, and may function through the activation of SIRT1.
研究领域:
内分泌学
·
肾病学
·
细胞生物学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2021
·
IF 6.165
Weichen Zhu,Yu Huang,Bo Jiang,Rengui Xiao
The strong self-discharge reaction is one of the key factors limiting the practical use of dual-ion batteries, which leads to low coulombic efficiency and discharge capacity loss. It is of great significance to effectively reduce the self-discharge effect through redox reactions between organic electrodes and electrolytes. Herein, a dual-ion battery study that uses 1,4,5,8-naphthalenetetracarboxylic dianhydride as the anode and 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide as the electrolyte is conducted. Due to the introduction of organic materials, the storage mechanism of cations on the anode is changed, which weakens the self-discharge effect. The battery shows a low self-discharge rate of 6.12% h −1 and a high discharge capacity of 114 mAh g −1 . The voltage declines by only 0.14 V after resting for 15 h due to the strong redox reaction between Pyr 14 + cations and the C O groups of NTCDA. Meanwhile, a double-layer filter paper system with small porosity is used to improve the cycle performance of the battery.
研究领域:
能源存储
·
电化学
·
材料科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）