INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
·
2026
·
IF 3.7
Xu Cheng,Yaxin Liu,Chenxu Zeng,Chang Liu,Zihan Zhang,Yanyuan Bao
Cholesterol transport plays a pivotal role in maintaining sterol homeostasis within eukaryotic cells. In mammals, Niemann-Pick Type C (NPC) proteins are known to participate in cholesterol transport. However, the precise pathways remain largely unexplored in insects. Here, we reported ML1, a lipid-recognition protein in a rice pest Nilaparvata lugens , specifically bound cholesterol. In addition, we identified a scavenger receptor class B Type Ⅰ (SRB1-12) protein as the receptor for ML1. SRB1-12 was predominantly expressed in the integument and regulated cuticular chitin biosynthesis and lipid metabolism in nymphs to ensure molting success. ML1 delivered cholesterol to SRB1-12 and supplied the steroid precursor for 20E biosynthesis, as reflected by altered whole-body 20E titers. ML1 and SRB1-12 co-regulated the expression of a newly identified gene MLSR , which played important roles in nymphal development and egg hatching. This study establishes a pathway of cholesterol trafficking within tissues and deepens the understanding of sterol homeostasis in insects.
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DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2019
·
IF 3.714
Yali Jiang,Yu Lu,Youyi Huang,Shouwen Chen,Zhixia Ji
Caffeine is a major component of tea and coffee. Apart from the stimulatory effects, cumulative intake of caffeine has caused several diseases. In addition, the presence of caffeine brings out the deterioration of environmental pollution. Therefore, the demand for decaffeinated products, especially decaffeinated coffee and tea, is increasing. However, Pseudomonas putida as the most widely studied bacteria isn't suitable for producing decaffeinated tea and coffee due to the production of an unpleasant odor and endotoxins at the same time. A more appropriate system ( Bacillus amyloliquefaciens HZ-12: ndmABCDE ) is needed to produce decaffeinated products; and HZ-12: ndmABCDE was constructed by orderly integrating N -demethylase genes ( ndmABCDE ) in a safer strain HZ-12. Demethylases (NdmABCDE) in P. putida can degrade caffeine to xanthine, which was further degraded to CO 2 and NH 4 + by the purine metabolic pathway. Finally, HZ-12: ndmABCDE could degrade 52.6% of caffeine in the optimized medium, 31.5% higher than HZ-12; caffeine was demethylated in sequence to form theobromine, paraxanthine, 7-methylxanthine and xanthine; and there are no endotoxins and unpleasant odor. This study provides a safer strain (HZ-12: ndmABCDE ) for producing decaffeinated tea and coffee and a new idea that B. amyloliquefaciens HZ-12 can heterologously express the demethylase genes in P. putida .
研究领域:
微生物学
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生物技术
·
环境科学
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DOI
高影响因子
Joule
·
2026
·
IF 37.1
Wenbo Cao,Chao Dong,Dongxing Kou,Chaofan Zheng,Zhiyang Wan,Longhui Lei,Jiajin Kuang,Yang Wang,Faisal Naveed,Liquan Cheng,Mengqi Jin,Zhaoyang Wang,Yingying Dong,Chong Chen,Mingtai Wang
Copper indium sulfide (CuInS 2 ) is a promising absorber among emerging chalcogenide photovoltaics. However, solution-processed CuInS 2 solar cells at low temperature (< 400°C) still lag far behind the requirement of at least 10% efficiency for practical application since 1993. Here, a low-temperature strategy, featuring a solution-processed In-rich CuInS 2 film interdigitated with electron-extracting interfaces and a sulfur anion-induced gradient phase transformation (S 2− -GPT) process at < 200°C, is conceptually developed for preparing the CuInS 2 solar cells with a breakthrough efficiency up to 12.28%. The interdigitated structure has unique advantages over the simple bilayer structure conventionally used; moreover, the S 2− -GPT process leads to the transformation from CuAu to CuIn 5 S 8 phase to reduce the defect density and charge recombination inside CuInS 2 film for boosting solar cell performance. The detailed principles behind material and device are elucidated. Our findings offer a promising way to chalcogenide solar cells and the application of CuInS 2 devices.
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 20
·16814-16822
·
IF 3.201
Wei Huang,Shucheng Liu,Jinxin Liu,Wenli Zhang,Jianming Pan
A facile strategy to fabricate crown ether (2-methylol-12-crown-4, 2M12C4) immobilized porous polymers (PGMA-CE) was reported toward lithium(I) (Li+) recovery. Macroporous polymer foam (polymeric glycidyl methacrylate, PGMA) with abundant epoxy groups was firstly prepared as a support using a high internal phase emulsion (HIPE) template. And then 2M12C4 was covalently attached onto the surface of PGMA to endow the PGMA-CE with good stability and high selectivity. PGMA-CE was characterized by SEM, FT-IR, XPS, and 13C NMR spectroscopy, and its porous structure and the successful introduction of 2M12C4 were confirmed. In batch mode experiments under optimum conditions, PGMA-CE exhibited fast adsorption kinetics (the equilibrium time was 3.0 h), and the kinetics data were well described using a pseudo-second-order model. A Langmuir-type monolayer adsorption was investigated, and the calculated maximum equilibrium adsorption capacity was 3.15 mg g−1. In addition, PGMA-CE displayed excellent selectivity for Li+ in the presence of multiple interfering ions (Na+, K+, Mg+, and Ca2+), and the selective separation factors (α) were above 4.75. Regeneration analysis showed that the adsorption capacity of PGMA-CE toward Li+ after five cycles was 91.8% of the first cycle.
研究领域:
材料科学
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环境工程
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DOI
高影响因子
Biomedical Materials
·
2020
·Vol. 15
·期 4
·045014
·
IF 3.174
Dong Han,Baolin Zhang,Lichao Su,Boning Yang
Efficient attachment of magnetic nanoparticles to cell membranes plays an important role in the activation of cell membrane channels. Streptavidin (SA) was successfully modified to Poly (ethylene imine) (PEI)-superparamagnetic iron oxide nanoparticles (SPIONs) to form SA/PEI-SPIONs, which have high colloidal stability and low cytotoxicity. The SA/PEI-SPIONs were incubated with PC-12 cells which had first been cultured in a Roswell Park Memorial Institute medium 1640 containing 0.2 mg l−1 biotin for 12 h. The cells were observed by transmission electron microscopy, and the nanoparticles were clearly attached on the cell membrane, which can be attributed to the specific binding between the SA and biotin sites on the cell surface. This work provides a simple way to attach SA-modified nanoparticles on the membranes of cells by only culturing cells in a biotin-containing medium. This work makes possible biomedical applications that require nanoparticles to target cell membranes.
研究领域:
纳米技术
·
生物医学工程
·
细胞生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Yong-Sheng Xu,Xin Liu,Kang-Kang Pei,Rui-Xiong Zhao,Guo-Liang Zhang,Qing Xia,Feng-Bao Zhang
The solubilities of naphthalene in decalin, octane, dodecane, dichloromethane, trichloromethane, carbon tetrachloride, 1,2-dichloroethane, o-dichlorobenzene, 1-octanol, 2-octanone, 4-methyl-2-pentanone, and benzene were measured by a dynamic method over the temperature range of 279 to 330 K at atmospheric pressure. By comparison, the experimental results indicated that the molality solubility in dichloromethane reached maximum at certain temperature. Then, the Apelblat equation, the λh equation, the Wilson equation, and the nonrandom two liquid (NRTL) equation were used to correlate the experimental data. The NRTL equation was regarded as the effective model for all the solvents. Finally, the mixing properties were also calculated based on the NRTL equation. All these data were particularly instructive for the process of recycling naphthalene.
研究领域:
物理化学
·
化工过程
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DOI
高影响因子
CRYOGENICS
·
2026
·
IF 2.5
Changkun Wang,Zuhua Chen,Jun Shen,Yong-Chang Lau,Heng Tu,Changmin Xiong
Single crystals of Ce 2 X 3 (NO 3 ) 12 ·24H 2 O (X=Cu, Co, CXN) were successfully synthesized, all crystallizing in the trigonal space group R ¯ 3 . Under a magnetic field variation of 0-9 T at 2 K, Ce 2 Cu 3 (NO 3 ) 12 ·24H 2 O and Ce 2 Co 3 (NO 3 ) 12 ·24H 2 O exhibit large magnetic entropy changes of 15.18 J·kg -1 ·K -1 and 16.19 J·kg -1 ·K -1 , respectively. Notably, the magnetic entropy changes of these novel crystals significantly exceed that of Ce 2 Mg 3 (NO 3 ) 12 ·24H 2 O (CMN), which is only 6.33 J·kg -1 ·K -1 under identical conditions. Meanwhile, the refrigerant capacity (RC), constrained-range equivalent refrigerant capacity product (RCP) and temperature-averaged entropy change (TEC) of CXN are also comprehensively superior to those of CMN. These performances suggest that CXN are promising candidates for ultra-low temperature magnetic refrigeration applications.
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高影响因子
Separations
·
2025
·Vol. 12
·期 2
·27
·
IF 2.7
Zhiyu Zhao,Lianjing Mao,Tianyu Zheng,Xiao Li,Chunsen Ye,Pengrui Zhang,Huifang Li,Wei Sun,Jinhe Sun
Enriched lithium isotopes (6Li and7Li) are essential in the nuclear energy industry, where6Li is bombarded with neutrons to produce tritium for fusion reactions, while7Li is used as a core coolant and pH regulator. Separation of6Li and7Li by electromigration is a promising method for producing enriched lithium isotopes that fulfill industrial needs. In this work, based on a previously proposed biphasic system electromigration routine, a three-stage system of ‘LiCl aqueous solution (anolyte)|B12C4-[EMIm][NTf2] organic solution|NH4Cl aqueous solution (catholyte)’ was constructed and the rules of lithium isotope separation and lithium-ion migration investigated. It was shown that the isotope enrichment effect of the catholyte was greatly affected by the experimental conditions, while that of the organic solution was less affected. As the B12C4 concentration increased, enhancement of7Li enrichment in the catholyte and6Li enrichment in the organic solution was observed, and α(C/O) and α(O/A) reached 0.975 and 1.018 at B12C4 of 0.5 mol/L. With the increase in current, migration time, and LiCl concentration, the isotope that was enriched in the catholyte trended from7Li to6Li (about 6 mA, 12 h or LiCl of 5 mol/L). Taking lithium-ion transport efficiency and lithium isotope separation effect into consideration together, a current of at least 6 mA, duration of at least 12 h, LiCl concentration of at least 1 mol/L and B12C4 concentration of 0.2 mol/L are suggested for the electromigration process. The work provides an important reference for system construction and experimental design of a biphasic electromigration separation method, which is expected to be an industrial alternative because of its environmental protection and high efficiency.
研究领域:
化学工程
·
核能技术
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DOI
高影响因子
ACS Omega
·
2025
·
IF 4.3
Yan Li,Miaomiao Jiang,Shuang Liang,Zhiqiang Chen,Pengfei Qiang,Lu Zhang,Xiwei Zhang,Shuoye Yang
Gold nanoparticles (AuNPs) have long been considered to be one of the safest central nervous system (CNS) drug delivery carriers. However, recent studies have shown that AuNPs can affect the mitochondria–lysosome interaction in neuronal cells, although the underlying mechanism is not fully understood. The mitochondria–lysosome interaction is crucial for mitochondrial damage repair and quality control. In this study, we used PC-12 cells as a neuronal cell model to investigate the impact of AuNPs on the regulation of mitochondria–lysosome interaction. We found that AuNPs disrupt this regulation by interfering with the expression of TBC1D15, a key regulator of the mitochondria–lysosome interaction. Specifically, the expected increase in TBC1D15 expression upon mitochondrial damage was reduced with increasing AuNP concentrations. Concurrently, we observed a significant increase in the number of mitochondria and the level of the mitochondria–lysosome interaction. Additionally, the level of cellular autophagy represented by LC3B was markedly enhanced. These findings suggest that AuNPs may lead to the accumulation of mitochondrial damage by interfering with TBC1D15, resulting in neurotoxicity. This study provides important theoretical support for elucidating the mechanism of AuNP-mediated disruption of the mitochondria–lysosome interaction, which is crucial for understanding the potential risks of using AuNPs as drug delivery carriers in the central nervous system.
研究领域:
细胞生物学
·
毒理学
·
神经科学
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DOI
PubMed
高影响因子
JOURNAL OF SOLID STATE CHEMISTRY
·
2020
·
IF 2.726
Wenfeng Zhou,Xiao-Hui Li,Wen-Dong Yao,Huaiguo Xue,Sheng-Ping Guo
Inorganic compounds with mixed anions attract broad interest recently in view of their rich structural chemistry and physical performance. Specifically, chalcogenide borates are receiving increasing attention though they are in their infancy. Here, the chalcogenide borate Zn 8 Se 2 (BO 2 ) 12 is revisited by focusing on its second-harmonic generation (SHG) activity. Its sodalite cage structure is elucidated in detail and correlates with the SHG behavior. Its optical band gap is determined to be 4.42 eV. Theoretical calculations on its electronic structure and optical properties are performed and the results are well consistent with the experimental ones. Besides, the structural chemistry with related structures and possible SHG improvement strategies are discussed.
研究领域:
无机化学
·
材料科学
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DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2021
·
IF 6.291
Yan Fu,Quansheng Wang,Liang Zhang,Shuping Ling,Huiyan Jia,Yinliang Wu
The residual behaviors and dietary risk probability of 12 pesticides in Dendrobium officinale Kimura et Migo cultivated at two representative locations under green house conditions were investigated using liquid chromatography-tandem mass spectrometry. Field trials showed that the half-lives of 12 pesticides ranged from 0.9 to 14.4 days in fresh D. officinale stems. Based on maximum residue levels (MRLs), the ultimate residues of imidacloprid, dimethomorph, metalaxyl, tebuconazole, and cyazofamid at a pre-harvest interval (PHI) of 28 days were within acceptable limits. For abamectin, indoxacarb, and difenoconazole, 35-day PHIs were needed. The PHIs of trifloxystrobin and fluopyram were 42 days, the time required for their residues to be reduced to an MRL of 4 mg/kg. The chronic and acute risk quotients of target pesticides at PHIs of 28–42 days were below 5.929% and 0.532%, respectively, showing that the evaluated D. officinale exhibited an acceptably low dietary risk to the general population.
研究领域:
植物学
·
环境科学
·
毒理学
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DOI
PubMed
高影响因子
Materials Research Express
·
2017
·Vol. 4
·期 7
·075020
·
IF 1.068
Bingying Gao,Shaoxiang Lu,Mulenga Kalulu,Olayinka Oderinde,Lili Ren
To replace traditional preparation methods of silica aerogels, a small-molecule 1,2-epoxypropane (PO) has been introduced into the preparation process instead of using ammonia as the cross-linking agent, thus generating a lightweight, high porosity, and large surface area silica aerogel monolithic. We put forward a simple solution route for the chemical synthesis of silica aerogels, which was characterized by scanning electron microscopy (SEM), TEM, XRD, FTIR, thermogravimetric analysis (TGA) and the Brunauer–Emmett–Teller (BET) method In this paper, the effect of the amount of PO on the microstructure of silica aerogels is discussed. The BET surface areas and pore sizes of the resulting silica aerogels can be freely adjusted by changing the amount of PO, which will be helpful in promoting the development of silica aerogels to fabricate other porous materials with similar requirements. We also adopted a new organic solvent sublimation drying (OSSD) method to replace traditional expensive and dangerous drying methods such as critical point drying and freeze drying. This simple approach is easy to operate and has good repeatability, which will further facilitate actual applications of silica aerogels.
研究领域:
材料科学
·
化学工程
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DOI
高影响因子
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