Scientific Reports
·
2023
·Vol. 13
·期 1
·1-11
·
IF 4.6
Lan Xiang,Qi Dongdong,Ren Hao,Liu Tao,Shao Hong,Zhang Jiantao
Damage to lamellar keratinocytes, an essential cellular component of the epidermal layer of hoof tissue, can have a detrimental effect on hoof health and the overall production value of dairy cows. We isolated and cultured cow lamellar keratinocytes using the Dispase II and collagenase methods. We purified them by differential digestion and differential velocity adherent methods at each passaging and identified them by keratin 14 immunofluorescence. We established an in vitro model of inflammation in laminar keratinocytes using LPS and investigated whether chicoric acid protects against inflammatory responses by inhibiting the activation of the TLR4/MAPK/NF-κB signaling pathway. The results showed that cow lamellar keratinocytes were successfully isolated and cultured by Dispase II combined with the collagenase method. In the in vitro inflammation model established by LPS, the Chicoric acid decreased the concentration of inflammatory mediators (TNF-α, IL-1β, and IL-6), down-regulated the mRNA expression of TLR4 and MyD88 (P < 0.01), down-regulated the expression of TLR4, MyD88, p-ERK, p-p38, IKKβ, p-p65, p-p50 (P < 0.05), and increased the IκBα protein expression (P < 0.05). In conclusion, Chicoric acid successfully protected cow lamellar keratinocytes from LPS-induced inflammatory responses by modulating the TLR4/MAPK/NF-κB signaling pathway and downregulating inflammatory mediators.
研究领域:
兽医学
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免疫学
·
分子生物学
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DOI
PubMed
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2024
·
IF 6.1
Chenghao Huang,Fei Huang,Xuke Wang,Dongru Wang,Jiawei Wang,Xinhua Zhan
Polycyclic aromatic hydrocarbons (PAHs) constitute a category of persistent organic contaminants that possess the potential to induce carcinogenic, teratogenic, and mutagenic consequences. Our previous findings have revealed that plant roots actively take up PAHs through co-transport with protons, and auxin can promote PAHs uptake by wheat roots. It remains unclear whether nitric oxide (NO), a signaling molecule involved in numerous physiological processes in plants and downstream of auxin, can affect PAHs uptake by plant roots. In our study, 50 μmol/L sodium nitroprusside (SNP) significantly enhanced phenanthrene uptake after 4 h of exposure. After the addition of SNP (50 μmol/L), the H + flux on root surface increased, and H + -ATPase activity was activated, indicating that exogenous NO promotes phenanthrene uptake by plant roots via activating H + -ATPase. By studying the effects of 50 μmol/L cyclic guanosine monophosphate (cGMP), 5 mmol/L Ca 2+ , and 50 μmol/L adenosine monophosphate (AMP) on phenanthrene uptake by ryegrass roots and measuring root calcium-dependent protein kinases (CDPK) activity, we demonstrated that exogenous NO promotes phenanthrene uptake through the signaling pathway of NO, cGMP, Ca 2+ , CDPK, 14-3-3 protein and H + -ATPase. The results contribute significant insights into elucidating the underlying mechanisms of NO modulating PAHs absorption by plant roots, thereby offering crucial strategies for advancing food safety measures and enhancing the phytoremediation potential of soils and waters contaminated with PAHs.
研究领域:
环境科学
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植物生理学
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植物修复
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土壤污染
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2017
·
IF 1.194
Hailong Zhang,Xiaoyan Huang,Jianmei Sun,Akira Yamamoto,Yang Gao
Application of curcumin in clinic was limited due to its poor solubility, stability and low bioavailability. The purpose of this study is to investigate the efficacy of some common excipients including Poloxamer 188 , polyvinyl pyrrolidone K30 (PVP K30), Tween 80 , Gelucire 4414 and polyethylene glycol 400 (PEG 400) for overcoming these limitations of curcumin. Results showed that these excipients were efficient in improving the solubility and stability of curcumin and moreover significantly increased curcumin absorption in a concentration-dependent manner after their administrations to rat lungs. Studies of pulmonary membrane damage evaluated by measuring amount of protein and activity of lactate dehydrogenase (LDH) released from rat lung demonstrated that these excipients at studied concentrations did not cause obvious membrane damage to lung tissues. The bio-efficacy studies showed that combinatorial treatment of curcumin and PEG 400 (45%, v/v) resulted in a therapeutic effect on the sepsis-induced acute lung injury (ALI) of rats, characterized by obvious reduction of lung W/D weight ratio, protein content, MPO activity, and alleviated pathological symptoms of lung tissues. These findings suggested that intrapulmonary delivery of curcumin combined with these excipients, especially 45% (v/v) PEG 400, was promising for treating pulmonary diseases including ALI.
研究领域:
药理学
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呼吸医学
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重症监护
✓ 已验证
产品关联
DOI
高影响因子
Arabian Journal of Chemistry
·
2015
·
IF 3.725
Vikramjeet Singh,Kuldeep Singh,Amita Nand,Huanqin Dai,Jianguo Wang,Lixin Zhang,Alejandro Merino,Jingsong Zhu
In order to increase the scope and utility of small molecule microarrays (SMMs) we have combined SMMs and SPRi to screen small molecule antagonists against protein targets. Several small molecules, including immunosuppressive drugs (rapamycin and FK506) and reported inhibitors (FOBISIN and Blapsin) of 14-3-3ζ proteins have been used to validate this technology. Furthermore, a small library of isatin derivatives have been synthesized and screened on developed platform against 14-3-3ζ protein. Three molecules, derived from the endogenous intermediate isatin termed, FZIB-35, FZIB-36 and FZIB-38 were identified as novel inhibitors which shows significant interaction with 14-3-3ζ. A mutation in the binding groove of 14-3-3ζ, (K49E), almost abolishes the binding of these compounds to 14-3-3ζ protein. To exclude the probability of false positives, two more purified proteins (PtpA and BirA) were also tested. Furthermore, in order to confirm the binding pocket specificity, competition assay against R18 peptide was also carried out on presented platform. We show that SMMs in combination with SPRi are a powerful method to identify lead compounds in high throughput manner without the need to develop an activity based assay.
研究领域:
化学
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生物化学
·
分子生物学
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药物发现
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DOI
高影响因子
RSC Advances
·
2014
·Vol. 4
·期 68
·36031-36035
·
IF 3.708
Anlian Zhu,Shukun Bai,Wei Jin,Ruixia Liu,Lingjun Li,Yang Zhao,Jianji Wang
In this work, it was found that acidic protic ionic liquid N,N-dimethylaminoethanol hydrosulfate ([DMEA][HSO4]) was an efficient and reusable catalyst for the one-pot synthesis of 14-aryl-14H-dibenzo[a,j]xanthenes under solvent-free conditions. This ionic liquid is very cheap, air and water stable, and can be easily recovered and reused for at least six cycles. The catalytic system described here is a green protocol since in the one-pot synthesis of 14-aryl-14H-dibenzo[a,j]xanthenes, the yield of the target compounds is excellent, operation and work-up procedures are simple, and no volatile organic solvents have been used. It is suggested that the synergetic effect of the cation and the anion of this ionic liquid is the main reason for the high catalytic activity and high chemo-selectivity. Scale-up experiments also suggest that this catalytic system has potential in industrial application.
✓ 已验证
DOI
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 79
·64358-64367
·
IF 3.84
Jian Cheng,Yan Dong,Hui Ma,Lixia Li,Zuliang Liu,Fengqi Zhao,Siyu Xu
A one-dimensional energetic complex (Na[CL-14·2H2O]) constructed from sodium and 5,7-diamino-4,6-dinitrobenzofuroxan (CL-14) was synthesized, and its crystal structure was analyzed by X-ray diffraction. The crystal belongs to a monoclinic system with space group P21/c. The complex possesses a one-dimensional coordination framework based on the [ONaN]n chain, which might result in its low sensitivity and high heat resistant. The thermal decomposition mechanism of Na[CL-14·2H2O] was predicted by means of TG-DSC, TG-MS and FTIR analyses. The thermal decomposition of Na[CL-14·2H2O] contains one endothermic and one exothermic processes in the temperature range of 25–500 °C with NaNCO, Na2CO3, H2O, NO and CO2 as the final products. The non-isothermal kinetic and thermodynamic parameters for the first exothermic process of the Na[CL-14·2H2O] have been studied, with the apparent activation energy, pre-exponential factor, entropy of activation, enthalpy of activation and free energy of activation of 280.7 kJ mol−1, 57.8, 282.7 J mol−1 K−1, 276.2 kJ mol−1 and 154.8 kJ mol−1, respectively. The impact sensitivity and friction sensitivity of Na[CL-14·2H2O] was tested according to general methods, with the values of 27 J and 360 N, respectively. Na[CL-14·2H2O] has been investigated as an energetic catalyst in ammonium nitrate (AN) by means of TG-DTG, TG-MS, DSC and extent of conversion (α)–T kinetic curve analyses. And the thermal kinetic constants for the catalytic and noncatalytic decomposition of AN samples were calculated using Kissinger's and Ozawa–Doyle's equations. The possible catalytic mechanism was also discussed and proposed. The results show that Na[CL-14·2H2O] decreases the peak temperature and activation energy value of the complete decomposition process for AN by 41.3 °C and 10.9 kJ mol−1, respectively. Furthermore, the thermal decomposition of AN in the presence of Na[CL-14·2H2O] starts at significantly lower temperature (about 30 °C) than that of pure AN. Apparently, Na[CL-14·2H2O] could be incorporated as a potential energetic catalyst in AN-based propellants.
研究领域:
化学
·
材料科学
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产品关联
DOI
高影响因子
Journal of Functional Foods
·
2017
·
IF 3.144
Guan-Lin Chen,Xin Zhang,Song-Gen Chen,Men-Di Han,Yong-Qing Gao
In order to explore the potential of different subtropical fruit leaves as natural sources of bioactive compounds, free, esterified and insoluble-bound forms of total phenolic contents (TPC) and total flavonoid contents (TFC) were determined. The antioxidant activities of fruit leaves was determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP), and trolox equivalent antioxidant capacity (TEAC) assays. Furthermore, several phenolic compounds were detected using ultra performance liquid chromatography (UPLC) technique. Rutin, (+)-catechin, isoquercitrin, quercitrin and quercetin were widely found in these fruit leaves. The highest TPC, TFC and antioxidant capacities were found in Averrhoa carambola , Dimocarpus longgana Lour, Litchi chinensis Sonn , Mangifera indica and Psidium guajava Linn . The results implied that these leaves are important natural sources of antioxidants for preparing functional food ingredients and preventing oxidative stress diseases.
研究领域:
植物学
·
食品科学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
Journal of Analysis and Testing
·
2020
·Vol. 4
·期 1
·1-12
·
IF 0
Bai Ya-Li,Hong Zhi-Dan,Zhang Tian-Yi,Cai Bao-Dong,Zhang Yuan-Zhen,Feng Yu-Qi
Abstract An improved method is described for the fast and sensitive determination of multiple endogenous steroid hormones in steroidogenic pathway by liquid chromatography–tandem mass spectrometry (LC–MS/MS), in which serum samples, after a simple protein precipitation and a derivatization by 2-hydrazinopyridine (HP), were subjected to LC–MS analysis. The protein precipitation of serum samples is achieved via adding acetonitrile, while the derivatization of endogenous steroid hormones in serum samples after protein precipitation is performed using 2-hydrazinopyridine as a derivatization reagent. The derivatives of the steroid hormones exhibit good detection sensitivity in LC–MS analysis. Linearity range of the improved method for the target steroid hormones is in two orders of magnitude and the correlation coefficients ( r ) are in the range from 0.9885 to 0.9998. The limits of detection (signal/noise = 3) range from 0.07 to 65.26 ng/mL. The recoveries are between 80.2 and 116.4% with acceptable intra- and inter-day relative standard deviations (RSDs) ranging from 0.3 to 18.9%. The improved method can be applied to explore the differences of steroid hormones between healthy controls and polycystic ovary syndrome patients. Graphic Abstract
研究领域:
医学分析
·
内分泌学
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产品关联
DOI
PROCESS BIOCHEMISTRY
·
2016
·
IF 2.529
Fang Fang,Bingye Dai,Guang Zhao,Hua Zhao,Chao Sun,Hui Liu,Mo Xian
Considering of the possible application of fatty acids secretion in industrial production process for reducing extraction costs, the effects of enhanced fatty acids secretion by knocking out acyl-CoA synthetases on the global regulation, cellular metabolism were investigated through integrated omics analysis. Firstly, functional category analysis of differential expression genes showed that most of the genes were associated with the cell cycle and biology process such as cellular component biogenesis, cell wall organization and so on. Secondly, the decreased mRNA levels of Tdh, Eno and Pgk1 in glycolysis in faa mutant YB526 were confirmed by the differential protein analysis. As described in previous reports, the decreased protein levels of Tdh, Eno and Pgk1 were resulted from the increase of 14-3-3 protein activity. However, the increased level of 14-3-3 proteins could not improve the biosynthesis of ergosterol in the acyl-CoA deficiency strain YB526 due to the toxic effects of fatty acids. Thirdly, the decrease of ergosterol and ceramide contents in cell envelopes can improve the permeation of membrane and fatty acids secretion in faa mutant YB526. This comprehensive omics analysis could be helpful in the rational design of robust yeasts for the production of free fatty acids or other biobased chemicals.
研究领域:
微生物学
·
分子生物学
·
系统生物学
·
工业生物技术
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产品关联
DOI
高影响因子
Frontiers in Pharmacology
·
2020
·
IF 4.225
Tsai Yun,Xia Changbo,Sun Zhongwen
Lung cancer is the most common malignant tumor and is the leading cause of cancer-related deaths worldwide. Extraction of bioactive substances from herbs is considered as an alternative method to traditional treatment. 6-Gingerol is a naturally occurring phenol found in ginger that can be used to treat tumors and suppress inflammation. To determine whether 6-Gingerol can be used as a therapeutic agent for tumors. In this study, tumor-bearing mice were used as an animal model and A549 as a cell model. Western blot was used to detect the expression of autophagy related proteins ubiquitin-specific peptidase 14 (USP14), Beclin1, microtubule-associated protein light chain 3 (LC3) and ferroptosis related proteins nuclear receptor coactivator 4 (NCOA4), ferritin heavy chain 1 (FTH1), transferrin receptor 1 (TfR1), glutathione peroxidase 4 (GPX4), activating transcription factor4 (ATF4) in vivo and in vitro. MTT and EdU were used to detect the viability of A549 cells. H&E and immunofluorescence were used to localize and detect the expression of proteins. The detection of reactive oxygen species was performed using fluorescence probes. It was found that the administration of 6-Gingerol decreased the expression of USP14, greatly increased the number of autophagosomes, reactive oxygen species (ROS) and iron concentration, decreased the survival and proliferation rate of A549 cells, and significantly decreased tumor volume and weight. The results indicate that 6-Gingerol inhibits lung cancer cell growth via suppression of USP14 expression and its downstream regulation of autophagy-dependent ferroptosis, revealing the function and efficacy of 6-Gingerol as a therapeutic compound in A549 and its possible mechanism of action.
研究领域:
肿瘤学
·
药理学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
CHROMATOGRAPHIA
·
2025
·
IF 1.3
Jiang Qiaoling,Wu Qiaoli,Chen Shuhang,Zeng Tianfu,Tong Xiaoyi
This study introduces a rapid LC–MS/MS method designed to simultaneously quantify 14 water-soluble vitamins and their metabolites in human serum. The protein precipitation with metaphosphoric acid and stable isotope dilution techniques are utilized in this method. An Agilent XDB C18 column and dynamic ionization mode switching are adopted, enabling the completion of a single sample analysis within 5 min. The method has undergone rigorous validation. The lower limits of quantification (LLOQs) range from 0.3 to 400 ng/mL, with recovery rates between 80 and 120%. Intra-day and inter-day relative standard deviations (RSDs) are below 15%. The matrix effects are controlled within ± 15%, and the analyte carryover is less than 20% of the LLOQ. The stability tests have demonstrated that samples remain stable at room temperature (25 °C) for 10 h, at − 20 °C for 7 days, and can withstand one freeze–thaw cycle. Extracted samples are stable in the autosampler (4 °C) for 24 h. The method was applied to serum samples from 150 healthy individuals. Non-parametric statistical analysis was carried out to establish clinical reference intervals, which provides valuable data for the clinical evaluation of vitamin and metabolite levels in serum.
研究领域:
临床医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Experimental and Therapeutic Medicine
·
2022
·Vol. 24
·期 1
·1-11
·
IF 2.751
Lingyun Zhang,Shan Zhang,Fangrong Chen
Proteasome 26S subunit, non-ATPase 14 (PSMD14) expression has been previously reported to be reduced in patients with pre-eclampsia (PE). The present study investigated the interaction network associated with the role of PSMD14 in PE. Reverse transcription-quantitative PCR (RT-qPCR) and western blotting were performed to determine the transfection efficacy following plasmid-based gene transfer of PSMD14 into HTR-8/SVneo cells. Cell proliferation was measured using an MTT assay and 5-ethynyl-2'-deoxyuridine staining. The expression of proliferation-related proteins, including Ki67 and PCNA, was determined using western blotting. Wound healing and Transwell assays were performed to measure cell invasion and migration, whilst the expression of migration-related proteins, including MMP2 and MMP9, was measured using western blotting. The angiogenesis of HUVECs following treatment with the HTR-8/SVneo cell culture supernatant was examined using tube formation assay. Following overexpression of Hes-related family BHLH transcription factor with YRPW motif 1 (HEY1) by transfection of pcDNA3.1 expression vector containing full-length human HEY1 or knockdown by transfection of shRNA plasmids targeting HEY1, the expression of HEY1 and PSMD14 was detected using RT-qPCR and western blotting. The potential interaction between HEY1 and the PSMD14 promoter was examined using dual-luciferase reporter and chromatin immunoprecipitation assays. PSMD14 overexpression was found to promote the proliferation, invasion, migration of HTR-8/SVneo cells and the angiogenesis of HUVECs following treatment with the HTR-8/SVneo cell culture supernatant, accompanied by enhanced expression of proliferation and migration-related proteins. Furthermore, the transcription factor HEY1 activated the expression of PSMD14. Knocking down HEY1 expression partially reversed the promoting effects of PSMD14 overexpression on the proliferation, invasion, migration, angiogenesis, proliferation and migration-related protein expression in trophoblasts. In conclusion, HEY1-activated PSMD14 promoted trophoblast proliferation, invasion and angiogenesis. Therefore, HEY1 and PSMD14 can be potential targets for PE treatment.
研究领域:
分子生物学
·
细胞生物学
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妇产科学
·
妇科
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）