BIOSENSORS & BIOELECTRONICS
·
2021
·
IF 10.618
Lihang Zhu,Shuang Li,Wenxin Liu,Jing Chen,Qiwen Yu,Zhen Zhang,Yi Li,Jun Liu,Xing Chen
As an oxidase stress biomarker, 3-nitrotyrosine is closely associated with many cardiovascular diseases. Thus, early diagnosis and real time detection of 3-nitrotyrosine at bedside are highly important. Herein, we developed a handheld electrochemiluminescence (ECL) analysis device, which integrates printed circuit board (PCB) for electrical stimulation and smartphone for optical signals readout. Fast and accurate determination of 3-nitrotyrosine was achieved with Antibody/Ru(dcpy) 3 2+ @AuNPs/MoS 2 modified Au electrode (Ab/Ru@AuNPs/MoS 2 ) for ECL analysis. The linear range of 3-nitrotyrosine detection was from 10 −8 mol/L to 10 −6 mol/L with a detection limit of 8.4 × 10 −9 mol/L. In addition, an Android application was developed to realize real time analysis of ECL emissions and results readout for detection. To confirm the usage of the device, spiked serum with different concentrations was tested and the results indicated the practical reliability and stability of this device. The operating procedure for ECL analysis in this device is extremely easy and electrical stimulation was adjustable from 0 V to 5 V for general ECL systems. Thus, we believe this handheld device for ECL analysis has extensive prospects for application in Point-of-care testing and health caring.
研究领域:
生物医学工程
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分析化学
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医学诊断
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DOI
PubMed
高影响因子
JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
·
2023
·Vol. 100
·期 8
·615-621
·
IF 2
Ting Xu,Qingru Zeng,Xin Liu,Shensheng Xiao,Xiaoming Jiang,Yuepeng Lu,Zhiyong Gong,Yonggui Gao,Min Fang
There are more and more studies on the detection method of 3-chloro-1,2-propanediol fatty acid esters (3-MCPD esters) at present, by comparing these methods for the determination of 3-MCPD esters. Indirect methods, which determine total amount of 3-MCPD after hydrolysis of the esters, have an advantage over direct methods. The existing indirect methods, however, may yield unreliable results or require long hours of alkaline methanolysis. In contrast, the indirect enzymatic hydrolysis method has mild conditions and more accurate results. In this study, we developed a reliable and rapid indirect method for determination of 3-MCPD esters. 3-MCPD esters were enzymolysis to 3-MCPD by indirect enzymatic hydrolysis method, and the conditions of enzymatic hydrolysis were optimized, the content of 3-MCPD after enzymatic hydrolysis was detected by gas chromatography–mass spectrometry (GC–MS) and the yield was calculated. Finally, the optimum conditions for enzymatic hydrolysis of 3-MCPD esters were determined. According to the optimal enzymatic hydrolysis condition, the contents of 3-MCPD esters in four food oils were determined. The method is simple and sensitive and can meet the requirement of 3-MCPD esters detection in general oils.
研究领域:
食品科学
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分析化学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Chang Li,Hanbing Jia,Mingyue Shen,Yuting Wang,Shaoping Nie,Yi Chen,Yongqiang Zhou,Yuanxing Wang,Mingyong Xie
The capacities of six antioxidants to inhibit the formation of 3-monochloropropane-1,2 diol (3-MCPD) esters were examined in this study. Inhibitory capacities of the antioxidants were investigated both in chemical models containing the precursors (tripalmitoyl glycerol, 1,2-dipalmitoyl-sn-glycerol, monopalmitoyl glycerol, and sodium chloride) of 3-MCPD esters and in oil models (rapeseed oil and sodium chloride). Six antioxidants, butylated hydroxytoluene (BHT), butylated hydroxy anisole (BHA), tert-butyl hydroquinone (TBHQ), propyl gallate (PG), l-ascorbyl palmitate (AP), and α-tocopherol (VE), were found to exhibit inhibiting capacities on 3-MCPD ester formation both in chemical models and in oil models. TBHQ provided the highest inhibitory capacity both in chemical models and in oil models; 44% of 3-MCPD ester formation was inhibited in the presence of TBHQ (66 mg/kg of oil) after heating of rapeseed oil at 230 °C for 30 min, followed by PG and AP. BHT, BHA, and VE appeared to have weaker inhibitory abilities in both models. VE exhibited the lowest inhibition rate; 22% of 3-MCPD esters were inhibited in the presence of VE (172 mg/kg of oil) after heating of rapeseed oil at 230 °C for 30 min. In addition, the inhibition rates of PG and VE decreased dramatically with an increase in temperature or heating time. The results suggested that some antioxidants, such as TBHQ, PG, and AP, could be the potential inhibitors of 3-MCPD esters in practice.
研究领域:
食品科学
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化学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2015
·
IF 2.858
Xuehui Li,Yongming Lu,Wenna Zhang,Shixiong Yuan,Liyuan Zhou,Liming Wang,Qiuying Ding,Dandan Wang,Weiwei Yang,Zhengnan Cai,Yan Chen
Polysaccharide TLH-3 from the fruit body of Tricholoma lobayense Heim has shown outstanding antioxidant activity. In order to further explore it, TLH-3 was successfully modified to obtain a sulfated derivative (STLH-3). The chemical characteristics of STLH-3 and TLH-3 were determined by high performance liquid chromatography and infrared spectroscopy. Antioxidant activity and cytotoxicity of the samples were investigated in vitro . The antioxidant activities of STLH-3 were significantly improved. In the four indicators of antioxidant activity, the 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and superoxide anion scavenging activities of STLH-3 have exceeded that of Vitamin C (Vc). The cytotoxicity against tumor cells including human cervical carcinoma cell line (HeLa) and human breast cancer cell line (MCF-7) of STLH-3 was obviously stronger than that of TLH-3. STLH-3 had no toxic on human embryonic lung fibroblasts (HELF) cells. These results suggested that STLH-3 would be a promising bioactive macromolecule for potential applications in the field of health care and pharmaceutical.
研究领域:
生物化学
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药理学
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DOI
PubMed
高影响因子
MOLECULES
·
2019
·Vol. 24
·期 15
·2769
·
IF 3.06
Xican Li,Xiaojian Ouyang,Minshi Liang,Dongfeng Chen
The biological process, 3-O-galactosylation, is important in plant cells. To understand the mechanism of the reduction of flavonol antioxidative activity by 3-O-galactosylation, myricetin-3-O-galactoside (M3OGa) and myricetin aglycone were each incubated with 2 mol α,α-diphenyl-β-picrylhydrazyl radical (DPPH•) and subsequently comparatively analyzed for radical adduct formation (RAF) products using ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-ESI-Q-TOF-MS) technology. The analyses revealed that M3OGa afforded an M3OGa–DPPH adduct (m/z873.1573) and an M3OGa–M3OGa dimer (m/z958.1620). Similarly, myricetin yielded a myricetin–DPPH adduct (m/z711.1039) and a myricetin–myricetin dimer (m/z634.0544). Subsequently, M3OGa and myricetin were compared using three redox-dependent antioxidant analyses, including DPPH•-trapping analysis, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide radical (PTIO•)-trapping analysis, and•O2inhibition analysis. In the three analyses, M3OGa always possessed higher IC50values than those of myricetin. Conclusively, M3OGa and its myricetin aglycone could trap the free radical via a chain reaction comprising of a propagation step and a termination step. At the propagation step, both M3OGa and myricetin could trap radicals through redox-dependent antioxidant pathways. The 3-O-galactosylation process, however, could limit these pathways; thus, M3OGa is an inferior antioxidant compared to its myricetin aglycone. Nevertheless, 3-O-galactosylation has a negligible effect on the termination step. This 3-O-galactosylation effect has provided novel evidence that the difference in the antioxidative activities of phytophenols exists at the propagation step rather than the termination step.
研究领域:
植物化学
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生物化学
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分析化学
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DOI
高影响因子
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
·
2021
·
IF 3.995
Le Han,Jiaqiang Liu,Peng Liu,Bohan Li,Xianliang Li,Yan Xu
Ratiometric fluorescent sensing materials based on dual-emissive luminescent centers have been widely researched due to their highly stable self-calibrating monitoring performance. In this work, Eu 3+ , Tb 3+ co-doped gadolinium molybdate [Gd 2 (MoO 4 ) 3 ] phosphors were successfully prepared through a facile precipitation and a following heat treatment process. Dual-emissive Gd 2 (MoO 4 ) 3 : Eu 3+ , Tb 3+ phosphor showed characteristic emission peaks at 592, 617, and 703 nm corresponding to the transitions of 5 D 0 – 7 F 1 , 5 D 0 – 7 F 2 and 5 D 0 – 7 F 4 for Eu 3+ ions, and the other emissions at 488 and 543 nm were ascribed to 5 D 4 – 7 F 6 and 5 D 4 – 7 F 5 transitions for Tb 3+ , respectively. The temperature sensitivities of Gd 2 (MoO 4 ) 3 : Eu 3+ , Tb 3+ based on a multimode fluorescence intensity ratio (FIR) technique were investigated for monitoring temperature changes in the range of 80–450 K. The maximum relative sensitivities of the four modes of FIR (I Tb 3+ /I Eu 3+ ) techniques were successfully achieved with range of 0.37–0.50% K −1 . The results also demonstrated the high reversibility of Gd 2 (MoO 4 ) 3 : Eu 3+ , Tb 3+ phosphor used as a self-calibrating thermometer for thermal-sensing application.
研究领域:
材料科学
·
光学
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DOI
高影响因子
Journal of Energy Storage
·
2024
·
IF 8.9
Haoyu Deng,Xueting Yang,Hanghang Chen,Dezhan Ye,Xiancai Jiang,Yazhou Chen,Chenyi Wang
The in-situ polymerization of 1,3-dioxolane (PDOL) in battery cells has attracted extensive attention, however, the differences of Lewis acid initiators on the DOL monomer conversion (C%) and electrochemical properties has been ignored. Here, it is demonstrated that compare with Sn(OTf) 2 Lewis acid initiator, the gelation of DOL is achieved at the lower addition of In(OTf) 3 . By optimizing their content in the gelation process, their C% are comparable which are 95.7 % and 93.5 % for In(OTf) 3 and Sn(OTf) 2 , respectively. However, the higher ionic conductivity (7.1 × 10 −4 S cm −1 at room temperature), electrochemical window (~4.5 V) and Li + transference number (0.42) are obtained for DOL initiated with In(OTf) 3 , due to its lower molecular weight which is helpful to the intermolecular Li + transport along PDOL main chain; In addition, the Li/Li symmetrical cell of In(OTf) 3 can be stably cycled for >500 h at 3 mA cm −2 , with an over-potential of 178mv. The storage and cycling impedances of half cells further proves the stable interface of In(OTf) 3 initiated DOL toward to lithium metal in comparison with those of Sn(OTf) 2 . Scanning electron microscopy results show a denser and thinner interface in the DOL catalyzed by In(OTf) 3 . Finally, the assembled Li/LiFePO 4 battery delivers specific discharge capacity and capacity retention rates of 115.5mAh g −1 and 85 %, respectively after 300 cycles at 1C. Through the initiator selection, the DOL synthesis and electrochemical properties can be tuned, which further broadens the design idea of PDOL based solid polymer electrolyte.
研究领域:
材料科学
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电化学
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DOI
高影响因子
JOURNAL OF CHEMICAL SCIENCES
·
2020
·Vol. 132
·期 1
·1-7
·
IF 1.406
DU ZHONGLIN,LI FENRUI,LI LI,LI RAN
Abstract Microwave-assisted one-pot quick synthesis to 1-monosubstituted 1,2,3-triazoles was achieved with good to excellent yields using the widely available arylboronic acids, sodium azide and 3-butyn-2-ols within 15 min. This method features high efficient and facile as organic azides, acetylene gas and harsh conditions were avoided. Graphic abstract Microwave-assisted one-pot quick synthesis to 1-monosubstituted 1,2,3-triazoles was achieved with good to excellent yields using the widely available arylboronic acids, sodium azide and 3-butyn-2-ols within 15 min.
研究领域:
有机化学
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药物化学
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合成方法学
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绿色化学
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杂环化学
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DOI
高影响因子
MOLECULAR CELL
·
2024
·
IF 14.5
Wei Luo,Yunyuan Xu,Jie Cao,Xiaoyu Guo,Jingdan Han,Yuanyuan Zhang,Yuda Niu,Meiling Zhang,Yi Wang,Guohua Liang,Qian Qian,Song Ge,Kang Chong
While it is known that temperature sensors trigger calcium (Ca 2+ ) signaling to confer cold tolerance in cells, less is known about sensors that couple with other secondary messengers. Here, we identify a cold sensor complex of CHILLING-TOLERANCE DIVERGENCE 6 (COLD6) and osmotin-like 1 (OSM1), which triggers 2′,3′-cyclic adenosine monophosphate (2′,3′-cAMP) production to enhance cold tolerance in rice. COLD6, which is encoded by a major quantitative trait locus (QTL) gene, interacts with the rice G protein α subunit (RGA1) at the plasma membrane under normal conditions. Upon exposure to chilling, cold-induced OSM1 binds to COLD6, kicking out RGA1 from interaction. This triggers an elevation of 2′,3′-cAMP levels for enhancing chilling tolerance. Genetic data show that COLD6 negatively regulates cold tolerance and functionally depends on OSM1 in chilling stress. COLD6 alleles were selected during rice domestication. Knockout and natural variation of COLD6 in hybrid rice enhanced chilling tolerance, hinting design potential for breeding. This highlighted a module triggering 2′,3′-cAMP to improve chilling tolerance in crops.
研究领域:
植物学
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遗传学
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2024
·
IF 4.5
Xue Zhang,Jiang Chen,Yuan Yin,Shijun Xiao,Rui Zhang,Haiyang Guo,Tong Yang,Tongyu Zhou,Siyan Zhang,Yang Yang,Caili Bi,Xiao-Jun Li
Targeted delivery of chemotherapeutic agents to the cancerous cells is of fundamental importance and the key step of the targeted delivery system design is to choice a specific receptor. Glypican-3 (GPC3) is a known cell membrane-associated oncofetal proteoglycan that is specifically up-regulated in the fast-growing hepatocarcinoma cells but rarely expressed in the normal healthy liver. Therefore, GPC3 may be a perfect targeting receptor for delivery treatment of hepatocellular carcinoma (HCC). In the present study, a GPC3 specific-targeting nanoliposomal drug delivery system was constructed with GPC3 targeting peptide (named as L5 peptide) modification and anticancer drug cantharidin encapsulation, which aims to realize cantharidin targeted treatment of liver cancer. In our study, nanoliposome specifically targeting GPC3 receptor was successfully constructed with particle size of 127.9 nm and a spherical shape. The targeted liposomes were stable under 4 °C or room temperature for almost 15 days. The drug release from L5-modified liposomes was controlled and delayed. Their targeted delivery properties were characterized by relatively more accumulation in the GPC3 highly expressed HepG2 cells than that of GPC3 lowly expressed Huh-7 cells. The competition results also indicated that the liposomes with L5 peptide modification could competitively bind to GPC3 receptor. Moreover, the liposomes showed colocalization with the lysosome after 3 h incubation. The L5-modified liposomal CTD had an augmented cell growth inhibition and tumor inhibition than that of the free CTD on HepG2 cells and GPC3 highly expressed tumor mice. Our study establishes a new strategy for improving HCC treatment and chemotherapeutic agent targeting delivery.
研究领域:
肿瘤学
·
药剂学
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DOI
高影响因子
MATERIALS LETTERS
·
2024
·
IF 2.7
Ying Chen,Minghao Li,Jing Huang,Hui Zeng,Linhua Xu,Jing Su
This paper presents the growth of large-sized CsCu(Br, Cl) 3 crystals using the aqueous solution evaporation method. The structure and the components of the crystals were analyzed. It was found that CsCuBr 3 is a direct bandgap material, while CsCuCl 3 is an indirect bandgap material, and the band gap of CsCu(Br, Cl) 3 halides can be adjusted in the range of 2.63–3.63 eV (PBE) by changing the ratio of Br - /Cl - . Besides CsCuCl 3, which has an absorption valley at 654 nm, the other CsCu(Br, Cl) 3 crystals show high absorption in the range of 200–1000 nm. Under UV excitation, the PL spectra show that CsCu(Br, Cl) 3 crystals exhibit red and blue emission.
研究领域:
材料科学
·
光学
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DOI
高影响因子
ChemistrySelect
·
2025
·Vol. 10
·期 36
·e03645
·
IF 2
Miaomiao Tian,Qingwen Zheng,Yuanyuan Pang,Fangjun Wang,Gang Liu
In this study, we developed a novel and facile fluorescent turn-on sensing method for the selective detection of Cu 2+ ions based on the specific oxidative reaction between Cu 2+ ions and 3-carboxyphenylboronic acid (3-CPBA). In this reaction, 3-CPBA was oxidized by Cu 2+ to form 3-hydroxybenzoic acid, which emitted strong fluorescence emission at 412 nm. The resulting product was characterized using comprehensive spectroscopic analyses, including proton nuclear magnetic resonance ( 1 H NMR), carbon-13 nuclear magnetic resonance ( 13 C NMR), and electrospray ionization mass spectrometry (ESI-MS). These analyses confirmed the formation of 3-hydroxybenzoic acid as the primary product. Under optimal conditions, the proposed method could detect Cu 2+ in the range of 0.1–3 µM with a detection limit of 10.2 nM. The developed method exhibited high sensitivity, ease of operation, practicality, and good selectivity toward Cu 2+ over other metal ions. Furthermore, the practical applicability of the method was validated by successfully detecting Cu 2+ in real water samples, with good recoveries of 94.0–105.0%. Graphical A fluorescence turn-on method for Copper ions (Cu 2+ ) detection via oxidative hydroxylation of 3-carboxyphenylboronic acid (3-CPBA) was developed. Under alkaline conditions, Cu 2+ converted non-fluorescent 3-CPBA into highly fluorescent 3-hydroxybenzoic acid (λ ex/e m = 315/412 nm). The method achieved a 10.2 nM detection limit with linear response (0.1–3 µM), superior selectivity over competing metal ions, and validated performance in environmental waters.
研究领域:
分析化学
·
环境监测
·
生物传感
·
分子探针
·
金属离子检测
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）