FOOD CHEMISTRY
·
2023
·
IF 8.8
Yanmei Zhu,Fushuang Xiang,Yuanyuan Su,Xue Jiang,Yipeng Cang,Wanjun Long,Wei Lan,Gaoqiong Deng,Hengye Chen,Yuanbin She,Haiyan Fu
High - temperature Daqu Baijiu faces a challenge from illegal adulteration of high-grade Baijiu bottles with low-grade Baijiu, affecting its quality and value. This study developed a rapid identification method for high temperature Daqu Baijiu with the same aroma type using a four-channel visual array sensor and detection of color changes caused by competition coordination with Zn 2+ and color-changing organic dyes. The array sensor demonstrated high stability and repeatability in targeting flavor components and achieved 97.78 % or more accuracy combined with dd -SIMCA model in detecting adulteration across the Baijiu with same aroma type. The results of GC–MS and Quantum Chemical Calculation showed that esters, acids, and pyrazines played a crucial role. The smart phone App could quickly identify the authenticity of Baijiu with accuracy achieved 93 %. This research provides a foundation for rapid and reliable assessment of Baijiu quality and authenticity, enabling the industry to combat fraudulent practices effectively.
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高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Mingyuan Chi,Jichun Liu,Lianxin Li,Yuewen Zhang,Meng Xie
Alzheimer’s disease (AD) has a complex etiology and diverse pathological processes. The therapeutic effect of single-target drugs is limited, so simultaneous intervention of multiple targets is gradually becoming a new research trend. Critical stages in AD progression involve amyloid-β (Aβ) self-aggregation, metal-ion-triggered fibril formation, and elevated reactive oxygen species (ROS). Herein, red blood cell membranes (RBC) are used as templates for the in situ growth of cerium oxide (CeO2) nanocrystals. Then, carbon quantum dots (CQDs) are encapsulated to form nanocomposites (CQD-Ce-RBC). This strategy is combined with photothermal therapy (PTT) for AD therapy. The application of RBC enhances the materials’ biocompatibility and improves immune evasion. RBC-grown CeO2, the first application in the field of AD, demonstrates outstanding antioxidant properties. CQD acts as a chelating agent for copper ions, which prevents the aggregation of Aβ. In addition, the thermal effect induced by near-infrared laser-induced CQD can break down Aβ fibers and improve the permeability of the blood–brain barrier. In vivo experiments on APP/PS1 mice demonstrate that CQD-Ce-RBC combined with PTT effectively clears cerebral amyloid deposits and significantly enhances learning and cognitive abilities, thereby retarding disease progression. This innovative multipathway approach under light-induced conditions holds promise for AD treatment.
研究领域:
神经科学
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材料科学
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生物医学工程
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治疗学
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PubMed
高影响因子
NEUROSCIENCE
·
2023
·
IF 3.3
Lu-Lu Lin,Gui-Jun Song,Hui Zhang,Yan Yin,Shi-Meng Xin,Li Ding,Yu Li
Alzheimer's disease (AD) is a serious neurodegenerative disease characterized by amyloid-β (Aβ) aggregation and neuroinflammation. G-protein-coupled receptor 34 (Gpr34) was found highly expressed in the hippocampus of APP/PS1 mice. However, its role in AD remains unclear. Herein, the role of Gpr34 as well as its molecular mechanism was explored. Data in GSE85162 were analyzed and the differently expressed genes in the hippocampus tissues of APP/PS1 mouse model of AD were subjected to GO, KEGG and GSEA enrichment analyses. APP/PS1 mice were used as an animal model of AD and the cognitive impairment was evaluated by a water maze test. The level of Gpr34 in hippocampus and BV-2 cells as well as the activation of ERK/NF-κB signal was determined by quantitative real-time PCR, western blot or immunofluorescence. Our results showed that, in BV-2 cells exposed to Aβ 1-42 , Gpr34 knockdown decreased the levels of TNF-α, IL-1β, IL-6 and iNOS and suppressed the activation of ERK/NF-κB signal. Moreover, the Gpr34-overexpression-induced activation of ERK/NF-κB signal and up-regulated levels of TNF-α, IL-1β, IL-6 and iNOS were abolished by FR180204, an ERK inhibitor. On the other hand, the in vivo study showed that Gpr34 knockdown ameliorated the cognitive impairment in APP/PS1 mice, decreased the levels of TNF-α, IL-1β and IL-6, the activation of microglia and ERK/NF-κB signal. In conclusion, Gpr34 knockdown relieved cognitive deficits in APP/PS1 mice and suppressed neuroinflammation and microglial activation, maybe via the ERK/NF-κB signal. It is indicated that the high level of Grp34 in hippocampus may contribute to the pathogenesis of AD.
研究领域:
神经科学
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分子生物学
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神经退行性疾病
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PubMed
高影响因子
POLYMER BULLETIN
·
2026
·Vol. 83
·期 7
·401
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IF 4.2
Liu Deyu,Ba Xuan,Wu Shaoyuan,Hu Jinghan,Duan Jihong,Guan Xingyu,Zhou Guoyong,Wang Kui,Huang Weijiang,Yan Wei
Waterborne polyurethane (WPU) has the advantage of good flexibility in the coating film, high and low-temperature resistance, and the advantages of environmental protection. Currently, there is a high demand for flame-retardant WPU. Herein, a flame-retardantcomposites (WPU/BN@APP/DIDOPO) is successfully developed by incorporating ammonium polyphosphate (APP) modified boron nitride (BN@APP) and DOPO derivatives into WPU via simple casting technology.The results indicate that BN@APP formed the intended composite structure and was well dispersed in the WPU matrix. This system significantly enhanced the char residue at 700 ℃ from 0.23% to 5.22% and reducing the peak heat release rate (pHRR) and total heat release (THR) by 58.9% and 36.9%, respectively, compared to pure WPU. Performance analysis shows that during combustion, a dense and stable expanded carbon layer forms, effectively delaying heat and mass transfer. The mechanical properties of the WPU/0.5BN@APP/5DIDOPO composite material are optimal and its breaking strength and elongation at break are superior to those of components. This work demonstrates the potential of the BN@APP/DIDOPO composite system for enhancing flame retardancy in polymeric materials, providing valuable insights for designing advanced flame-retardant systems.
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高影响因子
COMPOSITES COMMUNICATIONS
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2025
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IF 7.7
Ruofan Yang,Yiqing Fu,Jinghang Zhu,Guoqing Li,Chao Yan,Yiqun Fang,Xiaoyan Bai,Weihong Wang,Yongming Song,Qingwen Wang
Ammonium polyphosphate (APP) has been attracting considerable attention from researchers due to its favorable physicochemical properties, including excellent flame retardancy, competitive price and nontoxicity. It is widely used in flame retardancy of wood plastic composites (WPCs). The biggest challenge currently is that the extensive addition of APP often leads to the generation of smoke and a decrease in mechanical performance, which severely limits its application in the fields of building materials. Two-dimensional (2D) layered double hydroxide (LDH) containing the metal cation has excellent catalytic effects and smoke suppression during combustion. However, the agglomeration of LDH severely affects its dispersity, thus limiting its flame retardancy. Herein, a novel 2D/2D MMT/LDH nanosheet was synthetized via a self-assembly technique. Then, MMT/LDH nanosheets were subsequently used in conjunction with APP to synergistically endow WPCs with excellent flame retardancy, smoke suppression and mechanical properties. Compared with pure WPCs, the addition of flame retardant reduced the pHRR, THR, and TSP release of the WPCs by up to 35.6 %, 21.0 %, and 13.8 %. Additionally, the mechanical properties of the flame-retardant WPCs showed slightly improvement compared to pure WPCs composites. Consequently, this work proposes a synergistic strategy for preparing flame retardant WPCs by integrating phosphorus and nitrogen-containing flame retardant with 2D/2D MMT/LDH nanosheets.
研究领域:
材料科学
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复合材料工程
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高影响因子
JOURNAL OF FISH BIOLOGY
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2024
·Vol. 105
·期 4
·1086-1108
·
IF 1.7
Cao Thang Luong,Gilbert Audira,Kevin Adi Kurnia,Chih-Hsin Hung,Chung-Der Hsiao
This paper introduces the Fish 3D Locomotion app (F3LA), a Python-based, Graphical User Interface (GUI)-equipped tool designed to automate behavioral endpoint extraction in zebrafish locomotion assays. Building on our previous work, which utilized a specialized aquatic tank with a mirror and a single camera for fish movement tracking in three dimensions, F3LA significantly enhances data processing efficiency. Its accuracy was tested by reanalyzing and comprehensively comparing the calculated data with the previously published data from prior publications. From the comparison results, 90% of endpoints showed a similar statistical difference result. These minor differences were due to the different starting points for the dataset and updated calculation formulas that are implemented in F3LA. In addition, shoaling area or shoaling volume calculations are also included in F3LA as a new feature that can serve as sensitive indicators of social cohesion, group dynamics, or stress responses, offering insights into neuropsychological conditions or the effects of pharmacological interventions. Furthermore, F3LA offers a marked improvement over manual operations, being at least five times faster, while maintaining consistent accuracy as it reduces human-induced errors, ensuring a higher degree of reliability in the results. Finally, the potency of F3LA was tested to evaluate the toxicities of 14 rare earth elements (REEs) to the adult zebrafish behaviors. Based on the results, our findings suggested that each tested REE altered fish behaviors in different patterns and magnitudes to each other. However, among the tested light rare earth elements (LREEs), neodymium was demonstrated to cause more relatively severe behavior alterations than other LREEs, indicated by the statistically higher value of entropy (0.2695 ± 0.04977 (mean with a standard deviation)) than the control group (0.2352 ± 0.05896). Meanwhile, in terms of heavy rare earth elements (HREEs), erbium seemed to lead to more distinct behavior toxicities than other HREEs, which was shown by the statistically lower level of fractal dimension (2.022 ± 0.3412) than the untreated group (2.255 ± 0.1661). Taken together, F3LA's development marks a significant advance in high-throughput toxicological and pharmacological assessments in zebrafish, leveraging three-dimensional locomotion data for a more comprehensive analysis of fish behavior performance, providing a significant contribution to research in various fields.
研究领域:
生物学
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毒理学
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药理学
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神经科学
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DOI
PubMed
高影响因子
ACS Applied Polymer Materials
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2026
·
IF 5.2
Zhenyang Yin,Yitong Jiang,Fengyu Meng,Xiaoming Qi,Jiangsheng Chen,Hongbo Dai,Chen Qian,Yaqin Fu
Flexible polyurethane foam (FPUF) suffers from high flammability and environmental concerns, necessitating sustainable fire protection strategies. This work highlights a nacre-inspired, halogen-free flame-retardant coating with a hierarchical multicomponent “brick–mortar” architecture. Ammonium polyphosphate (APP) and sodium montmorillonite (MMT) act as rigid multiscale “bricks”, while Schiff base-modified chitosan (DCS) cross-linked with gelatin (GEL) forms the adhesive “mortar”, constructing a hydrogen-bonded, organic–inorganic hybrid network with robust interfacial compatibility on FPUF. The resulting FPUF/3A@M-D/G composite demonstrates remarkable fire safety, achieving a limiting oxygen index (LOI) of 32.6% and a UL-94 V-0 rating. Cone calorimetry shows significant reductions in pHRR1 (−46.1%) and pHRR2 (−67.7%), and THR (−16.8%), along with prolonged ignition time (from 4 to 6 s) and increased char yield (from 8.2 to 26.3 wt %). Meanwhile, the coating improves compressive strength (from 7.96 to 9.32 kPa) without compromising elasticity. Mechanistic analyses (SEM, FTIR, Raman, XPS, and Py-GC/MS) reveal a dual flame-retardant action of condensed-phase char reinforcement and gas-phase radical inhibition. This scalable bioinspired strategy provides a halogen-free pathway to simultaneously improve flame retardancy and mechanical robustness of FPUF for applications in transportation, furniture, and energy systems.
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2025
·
IF 4.6
Jingjiao Xie,Huiyuan Gong,Zhihan Du,Chen Hong,Wen Luo
Hydrogen sulfide (H 2 S) plays an important role in various pathophysiological processes, and its concentration is associated with various diseases. As such, the development of new H 2 S probes for the early detection of these diseases is of great significance. To date, a variety of probes have been developed for H 2 S detection, however, most probes exhibit response to other sulfur-containing compounds, resulting in a lack of specificity and weaker anti-interference performance. In this paper, a new flavone-based near-infrared (NIR) fluorophore was synthesized by introducing a 5-[4-(dimethylamino) phenyl] thiophene group at the 2-position of the chromone structure. Moreover, using nitrobenzoate as recognition group, two novel probes (TF-3NO 2 and TF-4NO 2 ) were synthesized to detection of H 2 S in biological systems. Among them, TF-3NO 2 exhibited a more specific fluorescent response to H 2 S compared to TF-4NO 2 . Upon treatment with H 2 S, the fluorescence intensity showed a significant increase, showcasing the high specificity, strong anti-interference capability, near-infrared emission (690 nm), and large Stokes shift (∆λ = 223 nm) of the probe. Moreover, the probe was successfully applied for imaging both exogenous and endogenous H 2 S in cells and mice, and enabled to distinguish changes in the H 2 S concentration in the brains of APP/PS1 transgenic and wild-type mice. The above results indicate that TF-3NO 2 can penetrate the mouse blood brain barrier, and is expected to be a powerful molecular tool for detecting H 2 S in living systems within basic and biomedical research fields.
研究领域:
生物化学
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分子生物学
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生物医学工程
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神经科学
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药物化学
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DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2018
·
IF 3.831
Huang Xianfeng,Wang Jing,Chen Xiao,Liu Pan,Wang Shujin,Song Fangchen,Zhang Zaijun,Zhu Feiqi,Huang Xinfeng,Liu Jianjun,Song Guoqiang,Spencer Peter S.,Yang Xifei
Alzheimer’s disease (AD) is a progressive neurodegenerative disorder that has proved refractory to drug treatment. Given evidence of neuroprotection in animal models of ischemic stroke, we assessed the prenylflavonoid xanthohumol from the Common Hop (Humulus lupulus L.) for therapeutic potential in murine neuroblastoma N2a cells stably expressing human Swedish mutant amyloid precursor protein (N2a/APP), a well-characterized cellular model of AD. The ELISA and Western-blot analysis revealed that xanthohumol (Xn) inhibited Aβ accumulation and APP processing, and that Xn ameliorated tau hyperphosphorylation via PP2A, GSK3β pathways in N2a/APP cells. The amelioration of tau hyperphosphorylation by Xn was also validated on HEK293/Tau cells, another cell line with tau hyperphosphorylation. Proteomic analysis (2D-DIGE-coupled MS) revealed a total of 30 differentially expressed lysate proteins in N2a/APP vs. wild-type (WT) N2a cells (N2a/WT), and a total of 21 differentially expressed proteins in lysates of N2a/APP cells in the presence or absence of Xn. Generally, these 51 differential proteins could be classified into seven main categories according to their functions, including: endoplasmic reticulum (ER) stress-associated proteins; oxidative stress-associated proteins; proteasome-associated proteins; ATPase and metabolism-associated proteins; cytoskeleton-associated proteins; molecular chaperones-associated proteins, and others. We used Western-blot analysis to validate Xn-associated changes of some key proteins in several biological/pathogenic processes. Taken together, we show that Xn reduces AD-related changes in stably transfected N2a/APP cells. The underlying mechanisms involve modulation of multiple pathogenic pathways, including those involved in ER stress, oxidative stress, proteasome molecular systems, and the neuronal cytoskeleton. These results suggest Xn may have potential for the treatment of AD and/or neuropathologically related neurodegenerative diseases.
研究领域:
神经科学
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分子生物学
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药理学
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DOI
PubMed
高影响因子
Neuropsychiatric Disease and Treatment
·
2022
·
IF 2.989
Ting Zhang,Keli Dong,Lan Xiao,Guangcheng Li,Zhanwei Zhang
Objective We investigated the effect of icariin (ICA) combined with Panax notoginseng saponins (PNS) on intestinal microbiota and hippocampal protein expression in amyloid precursor protein/presenilin 1 (APP/PS1) transgenic mice, a model of Alzheimer’s disease (AD).Methods Transgenic mice were treated with icariin and PNS. The Morris water maze (MWM) was used to assess spatial memory, and the gut microbiota and differential protein expression in the hippocampus were investigated using high-throughput screening techniques. Differential protein expression was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting.Results The MWM results showed that the mice treated with the medium dose of ICA+PNS spent significantly more time in the target quadrant compared with the AD group. Bacterial diversity was the lowest in the AD group, with significantly greater diversity in the ICA + PNS treatment group. Three proteins were selected for proteomic analysis, and qRT-PCR and Western blot were used to detect the expression of 2ʹ-5ʹ-oligoadenylate synthetase ubiquitin like 1 (Oasl1), trichoplein keratin filament-binding protein (TCHP), and tumor necrosis factor receptor associated 3-interacting protein 1 (MIPT3). Compared with control mice, MIPT3 expression was increased and Oasl1 and TCHP were reduced in the AD group. These abnormal protein expressions tended to normalization after treatment with medium dose of ICA and PNS.Conclusion Treatment with ICA and PNS ameliorated memory impairment in an AD mouse model. The mechanisms may be related to modulation of the intestinal microbiota and expression of Oasl1, TCHP, and MIPT3.
研究领域:
神经科学
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药理学
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微生物学
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蛋白质组学
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DOI
PubMed
高影响因子
ACS Sensors
·
2025
·
IF 9.1
Jing Yang,Xiaoye Feng,Haiqin Li,Pengtao Chang,Xiaojun Ren,Xiaochun Li
Chronic kidney disease (CKD) has become a public health burden, with a high mortality rate and a trend toward youthfulness. It is imperative to develop a home monitoring system for the early diagnosis and long-term monitoring of CKD serum biomarkers. However, due to the limitations of conventional centrifugation preprocessing of whole blood samples, the real-time detection platform cannot fully meet the requirement of obtaining quantitative results directly from whole blood. Herein, a novel smartphone-assisted microfluidic chip integration of a whole blood separation point-of-care testing (POCT) system was proposed to achieve accurate quantification of CKD biomarkers uric acid (UA), creatinine (CR), and albumin (Alb) based on 30 μL of whole blood (fingertip blood). The whole blood separation module allows rapid separation of plasma from whole blood samples by vertical flow. A smartphone App for automated image recognition and processing was developed, which was applied for at-home routine healthcare. The detection limits of UA, CR, and Alb were 0.1127 mmol/L, 0.2978 μmol/L, and 0.7696 mg/mL, respectively, meeting clinical requirements. Additionally, the quantitative performance of 83 clinical whole blood samples showed high consistency with the clinical analyzer (Beckman AU 5400 automatic biochemical analyzer). This system is simple to operate and user-friendly, providing an accurate, rapid, cost-effective, and reliable detection method for the early screening and real-time monitoring of CKD, which holds significant potential in enhancing home self-management of CKD.
研究领域:
生物医学工程
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临床诊断
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微流控技术
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即时检测
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慢性肾病
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生物传感器
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家庭医疗健康技术
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DOI
PubMed
高影响因子
Frontiers in Aging Neuroscience
·
2020
·
IF 4.364
Zhao Yueyang,Long Zhimin,Ding Ya,Jiang Tingting,Liu Jiajun,Li Yimin,Liu Yuanjie,Peng Xuehua,Wang Kejian,Feng Min,He Guiqiong
Dihydroartemisinin (DHA) is an active metabolite of sesquiterpene trioxane lactone extracted from Artemisia annua, which is used to treat malaria worldwide. DHA can activate autophagy, which is the main mechanism to remove the damaged cell components and recover the harmful or useless substances from eukaryotic cells and maintain cell viability through the autophagy lysosomal degradation system. Autophagy activation and autophagy flux correction are playing an important neuroprotective role in the central nervous system, as they accelerate the removal of toxic protein aggregates intracellularly and extracellularly to prevent neurodegenerative processes, such as Alzheimer’s disease (AD). In this study, we explored whether this mechanism can mediate the neuroprotective effect of DHA on the AD model in vitro and in vivo. Three months of DHA treatment improved the memory and cognitive impairment, reduced the deposition of amyloid β plaque, reduced the levels of Aβ40 and Aβ42, and ameliorated excessive neuron apoptosis in APP/PS1 mice brain. In addition, DHA treatment increased the level of LC3 II/I and decreased the expression of p62. After Bafilomycin A1 and Chloroquine (CQ) blocked the fusion of autophagy and lysosome, as well as the degradation of autolysosomes (ALs), DHA treatment increased the level of LC3 II/I and decreased the expression of p62. These results suggest that DHA treatment can correct autophagic flux, improve autophagy dysfunction, inhibit abnormal death of neurons, promote the clearance of amyloid-β peptide (Aβ) fibrils, and have a multi-target effect on the neuropathological process, memory and cognitive deficits of AD.
研究领域:
神经科学
·
药理学
·
分子生物学
·
神经退行性疾病
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）