ENVIRONMENTAL RESEARCH
·
2024
·
IF 7.7
Cuicui Xu,Yue Lv,Xia Qin,Fanbin Zhang,Hongyun Ren,Xujie Lan,Xiyang Li,Xingwei Tao
Landfill leachate nanofiltration concentrates (LLNC) contain complex organic pollutants that are difficult to treat. This study developed a copper-doped attapulgite-chitosan composite catalyst (Cu@ATP-CTS) for efficient LLNC degradation in a Fenton-like system. The incorporation of attapulgite extended the effective pH range of Fenton reactions from 2 to 8, overcoming traditional limitations. Optimized via response surface methodology, the catalyst achieved 89.02% UV 254 removal, 73.86% COD removal, and 77.24% TOC removal within 115 min under optimal conditions. Copper played a crucial role in H₂O₂ activation, cycling between Cu 0 , Cu + , and Cu 2+ to generate hydroxyl radicals (·OH), the key species driving pollutant degradation. The Cu@ATP-CTS catalyst also demonstrated strong stability and reusability over five cycles. This study provides a robust and sustainable method for LLNC treatment, offering significant potential for application in landfill leachate and wastewater management.
研究领域:
环境科学
·
废水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Longrui Yang,Xiaoyun Sun,Jiaci Chen,Juan Zhang,Xiaoyu Li,Song Qu,Kai Wu,Fengchun Huang,Ailiang Chen
The somatic cell count (SCC) and total plate count (TPC) are essential quality indicators for raw milk. Traditional detection methods require separate measurements and rely on complex, large-scale instruments or cultivation techniques, which are both time-consuming and laborious. To address these challenges, this study developed a novel method for the simultaneous detection of SCC and TPC in the same raw milk sample using the ATP bioluminescence assay. This method utilizes oxy-ethylated iso-nonyl phenol (Neonol-10) and cetyltrimethylammonium bromide (CTAB) to selectively lyse somatic cells and microorganisms, respectively. This technique is straightforward to operate and can be completed within 2.5 h, with detection ranges of 1 × 10⁴ to 3 × 10⁶ cells/mL for SCC and 1 × 10⁵ to 5 × 10⁷ CFU/mL for TPC. Importantly, this technique meets the requirements of detection standards in China, European Union, Canada, United States, etc. For SCC or TPC in raw milk. Overall, this innovative approach does not rely on expensive equipment or facilities and the stepwise reagent addition procedure can be easily developed into an automated high-throughput system for rapid on-site testing of SCC and TPC in raw milk.
研究领域:
乳品科学
·
分析化学
·
微生物学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Dongxia Li,Yanan Peng,Liangqing Lu,Delun Chen,Bingrong Wang,Qiumei Pu,Qionglin Zhou,Xinxin Xiao,Mingpeng Fu,Xiaohong Wang,Jinchun Tu,Hua Pei,Qiang Wu
Adenosine triphosphate (ATP), as a crucial signaling molecule, is of high potential diagnosis value for multiple diseases. However, current technologies for ATP detection struggle to satisfy the harsh conditions required for biospecimen tests, existing several limitations for clinical practical application. In this study, the DNA tile response system was designed and constructed by integrating nucleic acid aptamer, hybrid chain reaction (HCR), and DNA tile self-assembly technology to achieve accurate detection of ATP in body fluid specimens. The DNA tile response system, possessing high-order structure characteristic, could remove HCR false-positive products and conquer the high noise background to enhance the detection sensitivity. Under the optimal reaction parameters, this system achieved the low limit of detection (LOD) of 189.23 nM over a wide linear range and splendid detection specificity faced with multiple interference terms. Furthermore, this system, possessing simple storage conditions, long-term detection stability, and superior anti-interference performance, was sufficient to satisfy the detection requirements for complicated biological specimens in multi-scenarios. Ultimately, the DNA tile response system showed excellent diagnostic efficiency that could easily distinguish urinary tract infection patients and healthy controls through ATP quantification, as well as hypertension patients and healthy ones. This system provides a high-potential rapid detection tool for early diagnosis and dynamic monitoring of urinary tract infections and hypertension, holding enormous potential for clinical translation due to the superiorities of simple operation, general applicability, and low instrument requirements.
研究领域:
生物技术
·
医学诊断
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2023
·
IF 4.5
Yanzhu Liu,Ming Tu,Wei Lang,Qing Guo,Yongxiu Li,Qian-Yong Cao
A new amphiphilic polynorbornene-based copolymer P1 was designed and synthesized successfully by ring-opening metathesis polymerization (ROMP) of polyethylene glycol (PEG) containing monomer NB-PEG and ferreocene-anchored monomer NB-Fc . The copolymer P1 can bind with anionic uranine dye ( UD ) through multiple interactions to form no fluorescent P1 / UD nano-structure ensemble in aqueous solution. The P1 / UD ensemble can be used as indicator displacement assay (IDA) for ATP detection with a high sensitivity and selectivity. Upon the addition of ATP, the fluorescence is significant enhanced for displacement of UD from P1 / UD ensemble. The LOD value of P1 / UD ensemble toward ATP is as low as 35.9 nM. The P1 / UD ensemble exhibits an excellent photo-stability and biocompatibility, and has been successfully used for imaging ATP in living cells.
研究领域:
高分子化学
·
分析化学
·
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Lei Luo,Min Wang,Yuan Zhou,Dongliu Xiang,Qing Wang,Jin Huang,Jianbo Liu,Xiaohai Yang,Kemin Wang
For intracellular molecular detection, the appropriate probes should include the abilities to enter target cells noninvasively, target specific sites, and then respond to the analytes reliably. Herein, a ratiometric fluorescent DNA nanostructure (RFDN) was designed for mitochondrial adenosine triphosphate (ATP) imaging in living cells. The DNA nanostructure was constructed by continuous hybridization of two hairpin DNA strands (HS1-Cy3 and HS2-Cy5) under the initiation of the trigger. HS1-Cy3 and HS2-Cy5 contained split aptamer fragments of ATP and are labeled with a fluorescent donor (Cy3) and acceptor (Cy5), respectively. The RFDN integrated multiple split aptamer fragments and increased the local concentration of sensing probes. The binding of ATP to aptamer fragments on the RFDN shortened the distance between Cy3 and Cy5, resulting in obvious ratiometric signals (fluorescence resonance energy transfer). The RFDN showed good biocompatibility and can be internalized into cells in a caveolin-dependent endocytosis pathway. The co-localization imaging results indicated that the DNA nanostructure could target the mitochondria via Cy3 and Cy5. Moreover, the confocal imaging results showed that the intracellular ATP changes stimulated by drugs in living cells could be indicated by the RFDN. In this way, the RFDN is expected to be a simple, flexible, and general platform for chemo/biosensing in living cells.
研究领域:
分子生物学
·
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Rong Sheng Li,Jiahui Liu,Yuan Yan,Chuanyang Su,Tiancheng Lai,Yunwen Liao,Yuan Fang Li,Na Li,Cheng Zhi Huang
Förster resonance energy transfer (FRET) from fluorescent nanoparticles to fluorescent dyes is an attractive approach for bioanalysis in living cells. However, the luminescence of the nanoparticle donor/acceptor has not been effectively used to produce highly efficient FRET because the distance between the energy donor and energy acceptor is often larger than the effective FRET radius (about 10 nm) and the uncontrolled rotational and translational diffusion of luminophores. Here, we develop an aggregation-enhanced energy transfer strategy that can overcome the impedance for effective energy transfer. The functional nanoprobes, named TPP-CDs-FITC, are carbon dots (CDs) functionalized with triphenylphosphine (TPP) and ∼117 fluorescein 5-isothiocyanate (FITC) on the surface. In dispersed solution, the 3.8 nm TPP-CDs-FITC show weak FRET efficiency (15.4%). After TPP-instructed mitochondrial targeting, enhanced FRET efficiency (53.2%) is induced due to the aggregation of TPP-CDs-FITC selectively triggered by adenosine triphosphate (ATP) in the mitochondria. The enhanced FRET efficiency can be attributed to the joint effect of the augment of numbers of FITC acceptors within 10 nm from dispersed 117 to aggregated 5499 and the restricted rotational and translational motions of TPP-CDs donors and FITC acceptors. Ultimately, we successfully observe the fluctuations of ATP levels in the mitochondria using the aggregation-enhanced energy transfer strategy of the TPP-CDs-FITC nanodevice.
研究领域:
生物物理学
·
生物化学
·
细胞生物学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2021
·Vol. 43
·期 4
·992-1000
·
IF 6.15
Wu Xiao-yan,Lv Jin-yan,Zhang Shi-qing,Yi Xin,Xu Zi-wei,Zhi Yuan-xing,Zhao Bo-xin,Pang Jian-xin,Yung Ken Kin Lam,Liu Shu-wen,Zhou Ping-zheng
Dysregulation of NLRP3 inflammasome results in uncontrolled inflammation, which participates in various chronic diseases. TWIK2 potassium channel mediates potassium efflux that has been reported to be an essential upstream mechanism for ATP-induced NLRP3 inflammasome activation. Thus, TWIK2 potassium channel could be a potential drug target for NLRP3-related inflammatory diseases. In the present study we investigated the effects of known K2P channel modulators on TWIK2 channel expressed in a heterologous system. In order to increase plasma membrane expression and thus TWIK2 currents, a mutant channel with three mutations (TWIK2 I289A/L290A/Y308A ) in the C-terminus was expressed in COS-7 cells. TWIK2 currents were assessed using whole-cell voltage-clamp recording. Among 6 known K2P channel modulators tested (DCPIB, quinine, fluoxetine, ML365, ML335, and TKDC), ML365 was the most potent TWIK2 channel blocker with an IC 50 value of 4.07 ± 1.5 μM. Furthermore, ML365 selectively inhibited TWIK2 without affecting TWIK1 or THIK1 channels. We showed that ML365 (1, 5 μM) concentration-dependently inhibited ATP-induced NLRP3 inflammasome activation in LPS-primed murine BMDMs, whereas it did not affect nigericin-induced NLRP3, or non-canonical, AIM2 and NLRC4 inflammasomes activation. Knockdown of TWIK2 significantly impaired the inhibitory effect of ML365 on ATP-induced NLRP3 inflammasome activation. Moreover, we demonstrated that pre-administration of ML365 (1, 10, 25 mg/kg, ip) dose-dependently ameliorated LPS-induced endotoxic shock in mice. In a preliminary pharmacokinetic study conducted in rats, ML365 showed good absolute oral bioavailability with F value of 22.49%. In conclusion, ML365 provides a structural reference for future design of selective TWIK2 channel inhibitors in treating related inflammatory diseases.
研究领域:
药理学
·
免疫学
·
炎症性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Dan Zhao,Mingmin Jia,Meisheng Li,Hengyang Mao,Zhong Ma,Yijiang Zhao,Weihong Xing
Attapulgite/carbonylated graphene oxide (ATP/GO-COOH) composite membranes have been successfully prepared by vacuum-assisted filtration of an aqueous ATP/GO-COOH dispersion on a microfiltration substrate. Due to the interaction between the OH groups of ATPs and COOH groups of GO-COOH, ATP rods showed excellent compatibility with GO-COOH nanosheets , and the ATP mass fraction was significantly improved. The large amount of ATP can establish a fast transport channel for the diffusion of water molecules in the composite membrane. As a result, the optimum membrane gave a permeation flux with 13582 L/(m 2 h), which is significantly higher than that of the pure GO-COOH membrane (2281 L/(m 2 h)). In addition, the underwater superoleophobic properties of the ATP/GO-COOH composite membrane enable it to have a low oil-adhesive water/membrane interfaces. The ATP/GO-COOH composite membrane showed good separation and separation stability for oil-in-water emulsion systems with different concentrations, pH, and oil types.
✓ 已验证
产品关联
DOI
高影响因子
NPG Asia Materials
·
2014
·Vol. 6
·期 7
·e114
·
IF 9.902
Lin Youhui,Huang Yanyan,Ren Jinsong,Qu Xiaogang
There has been great interest in the development of artificial inorganic nanomaterials that mimic natural peroxidases. Unfortunately, these nanomaterials usually possess relatively low catalytic activity and are generally considered to work effectively only within a narrow temperature range (for example, they are inactive at high temperatures). Intriguingly, adenosine triphosphate (ATP) is not only the ubiquitous energy currency of life, it is also known to form charge transfer complexes with aromatic molecules and to participate in free radical redox chemistry. Inspired by its unique properties, for the first time, we reveal a novel catalytic role for ATP and show that ATP is an ideal boosting agent for markedly improving the catalytic activity of a peroxidase mimic over a broad temperature range and, more significantly, making it possible to achieve exceptionally efficient high-temperature catalytic reactions. These observations pave the way for identifying highly effective modulators that promote the overall performance of artificial enzymes.
研究领域:
生物化学
·
催化作用
·
纳米材料
·
酶工程
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2020
·Vol. 10
·期 1
·1-9
·
IF 3.998
Zhang Lei,Weng Yi-jin,Liu Xiao,Gu Wen,Zhang Xia,Han Lei
Surface Enhanced Raman Scattering (SERS) has been widely applied in many research fields such as biological detection and chemical analysis. However, for the common Au nanoparticles, it’s too hard to guarante the three aspects: the great enhanced effect, the controllable aggregation and the uniformity of nanoparticles, the environmental friendliness and biocompatibility of nanoparticles. In this paper, phytate acid (IP6)-coated Au nanoparticles (IP6@Au NPs) are more stable and have a higher enhancement factor than Au nanoparticles. In order to achieve the uniformity of the spherical IP6-coated@Au nanoparticles (IP6@Au NPs), IP6 was used as a soft template. In the presence of IP6, IP6@Ag nanoparticles were first synthesized by reducing AgNO 3 with trisodium citrate, then IP6@Au NPs were obtained by reducing HAuCl 4 with Ag nanoparticles. The IP6@Au NPs exhibit excellent Raman signal enhancement by using p-aminothiophenol (4-ATP) as the probe molecules. The effects of Fe 3+ on the performance of IP6@Au NPs SERS substrates were also studied. The results show that SERS has the best enhancement effect when adding proper amount of Fe 3+ (0.56 PPM), and the limit of detection was 10 −7 M 4-ATP.
研究领域:
纳米技术
·
分析化学
·
材料科学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
Chemical Science
·
2017
·Vol. 9
·期 5
·1184-1190
·
IF 8.668
Xiangdan Meng,Wenhao Dai,Kai Zhang,Haifeng Dong,Xueji Zhang
Herein, we design a smart autonomous ATP self-powered strand-displacement cascade amplification (SDCA) system for highly sensitive multiple intracellular miRNA detection. Rationally engineered Y-motif DNA structures are functionalized on mesoporous silica-coated copper sulfide nanoparticles loaded with numerous ATPs (CuS@mSiO2-Y/ATP) through pH stimulus-responsive disulfide bonds. The SDCA system is implemented by endogenous specific miRNA as a trigger and ATP as fuel released from the nanocarrier at acidic pH and photothermal stimuli-responsive CuS. The ATP self-powered SDCA process presents higher sensitivity compared to that without amplification for intracellular miRNA imaging. Two-color simultaneous and sensitive imaging of multiple cancer-related miRNAs in living cells is also confirmed. This enables facile and accurate differentiation between normal cells and different types of cancer cell using intracellular miRNA imaging, which improves the veracity and timeliness for early cancer diagnosis.
研究领域:
纳米技术
·
生物医学工程
·
分子生物学
·
癌症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2020
·
IF 8.352
Xiaoqin Zhu,Yan He,Zhongrui Liu,Ziting Zhu,Yuehua He,Jiewen Qiu,Danqiong Liu,Mingshu Mo,Peng Wang,Xiumei Tian,Pingyi Xu
Epilepsy is characterized by repeated spontaneous seizures and remains untreatable due to its complicate pathogenesis. Low-dose hydrogen sulfide (H 2 S) has been shown to exert antiepileptic and protective effects in the central nervous system, but the administration of H 2 S gas to suppress seizures is difficult. In the present study, we synthesized a safe and efficient carbazole-based H 2 S donor from aldehydes with a one-pot procedure and characterized this specific H 2 S donor via proton nuclear magnetic resonance ( 1 HNMR), scanning electron microscopy (SEM), and absorption spectroscopy. In vitro and in vivo risk assessments demonstrated that the H 2 S donor (up to a 400-μM concentration) had sufficient biocompatibility and membrane permeability and suppressed epileptic seizures. Importantly, the suppression of seizures was determined in the brain when the H 2 S donor was injected into the lateral ventricle in a rat model of advanced seizures. Furthermore, the mechanism by which the H 2 S donor suppressed the expression of seizures may have been related to H 2 S donor-induced increases in the expressions of the ATP-sensitive potassium channel (K ATP ) subunits, Kir6.2 and SUR1. Taken together, these assays suggested that our novel H 2 S donor may represent a potential therapeutic strategy for ameliorating seizures in epilepsy.
研究领域:
神经科学
·
药理学
·
医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）