COLLOIDS AND SURFACES B-BIOINTERFACES
·
2024
·
IF 5.4
Xuechun Kan,Jing Ma,Jun Ma,Dongdong Li,Fan Li,Yuyu Cao,Cheng Huang,Yan Li,Peidang Liu
Radiation therapy (RT) is one of the most effective and widely used treatment methods for glioblastoma multiforme (GBM). However, its efficacy is often compromised by the inherent radioresistance of tumor cells, while the restrictive nature of the blood-brain barrier (BBB) specifically impedes the delivery of radiosensitizer. Thus, we constructed and characterized polyethylene glycol (PEG)-functionalized silver-gold core-shell nanoparticles (PSGNPs) targeting both BBB (TfRA4) and GBM (DNA1) (TDSGNPs). Afterwards, studies conducted both in vitro and in vivo were employed to assess the BBB penetration capabilities, abilities of GBM targeting and radiosensitization effect. Transmission electron microscope images of PSGNPs showed a core-shell structure, and the results of ultraviolet-visible absorption spectroscopy and dynamic light scattering displayed that TDSGNPs were successfully constructed with excellent dispersion properties. TDSGNPs could be specifically taken up by U87MG cells and the uptake peaked at 24 h. TDSGNPs combined with RT obviously increased the apoptosis proportion of the cells. It was shown by the in vitro and in vivo investigations that TDSGNPs could target U87MG cells after crossing the BBB, and further study revealed that TDSGNPs showed an uptake peak in the tumor sites after 3 h intravenous injection. The radiosensitization of TDSGNPs was better than that of the nanoparticles modified with single aptamers and the median survival of tumor-bearing mice was greatly extended. This study demonstrated that TDSGNPs could penetrate BBB to target GBM, functioning as a promising radiosensitizer for the targeted therapy of GBM.
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高影响因子
SENSORS
·
2024
·Vol. 24
·期 24
·7878
·
IF 3.4
Xiaoyu Su,Junbiao Chen,Shanshan Wu,Yong Qiu,Yuxiang Pan
As a crucial biomarker for the early warning and prognosis of liver cancer diseases, elevated levels of alpha-fetoprotein (AFP) are associated with hepatocellular carcinoma and germ cell tumors. Herein, we present a novel signal-on electrochemical aptamer sensor, utilizing AuNPs–MXene composite materials, for sensitive AFP quantitation. The AuNPs–MXene composite was synthesized through a simple one-step method and modified on portable microelectrodes. As signal molecules, AFP aptamers were conjugated with methylene blue (MB) and immobilized on the electrode surface. When interacting with AFP, conformational changes in the aptamer–target complex caused MB to approach the electrode, and the electrochemical signal was enhanced through signal-on mechanisms. The developed sensor demonstrated high sensitivity and selectivity for AFP, with a log-linear relationship defined as 1–300 ng/mL, and the LOD was 0.05 ng/mL (S/N = 3). The method was applied to laboratorial and real clinical samples and presented satisfactory selectivity, reproducibility, and long-term stability. The proposed high-performance sensor highlights the potential of electrochemical aptamer sensors in improving the warning capabilities in disease management.
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Analytical Methods
·
2024
·
IF 2.7
Weiqiang Li,Xiaoxue Liu,Pianpian He,Wentao Hu,Kaijie Tang,Yangping Wen,Qian Zeng,Huiyi Tang,Yaxuan Lei,Xin Liu
Deoxynivalenol (DON) has drawn considerable attention for its obvious pathogenicity and wide use in agro-products, which cause a potential threat to human health. In this work, an electrochemical immunosensor is developed for the highly sensitive and selective detection of DON in wheat flour using AuNPs-BP-MWCNTs-COOH and antibodies. The AuNPs-BP-MWCNTs-COOH nanocomposite was prepared via an in situ reduction reaction and ultrasonic-assisted liquid-phase exfoliation. The nanocomposite exhibits a larger surface area, decent stability, excellent electron transfer capability, good protein binding capability and prominent specificity. The plentiful carboxyl group on the nanocomposite can bind to the amino group of the antibody, and AuNPs have an affinity for the sulfhydryl group of the antibody, which makes it feasible for the nanocomposite to load the antibody. The peak currents are plotted against the logarithm of DON concentration from 0.002 to 80 ng mL−1 with a limit of detection (LOD) of 0.5 pg mL−1. This approach establishes an effective label-free immunosensor platform for the detection of DON with high sensitivity and selectivity in various food and agricultural products.
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Chemistry-An Asian Journal
·
2025
·
IF 3.3
Xiaoqian Zhou,Haicheng Song,Xinying Zhang,Lifei Chen,Yingying Chen,Zhenzhen Li,Lingyan Feng
MXene, a two-dimensional nanomaterial, has metal conductivity, high electronegativity, functionalized with surface groups, which make it widely applicable in catalysis and biosensing. However, studies on the principle of enhanced electrochemiluminescence (ECL) by MXene composites and the improvement of their performance in catalyzing the ECL reaction are still in their infancy. In this study, gold nanoparticles (AuNPs) are obtained by mild reductive reduction and loaded in situ on the Ti 3 C 2 T x MXene surface to form the composites (AuNPs@MXene). In oxygenated PBS test buffer, AuNPs@MXene enhance the ECL emission of silver nanoclusters (AgNCs) with aggregation-induced electrochemiluminescence (AIECL) properties as luminophore. Approximately 7.5-fold enhancement of ECL signals is obtained by using two ECL enhancement strategies: an efficient AIECL emitter and a co-reaction accelerator. The special nucleic acid structure with “Three Way Junction (TWJ)” enables an ultra-sensitive detection of microRNA, providing an efficient and ultra-sensitive method for microRNA detection. The biosensor achieves a wide detection range of microRNA-21 from 100 aM to 1 nM, with a low detection limit of 31 aM, and exhibits excellent stability, selectivity and high reproducibility in real samples.
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高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-10
·
IF 5.3
Yang Xiao,Li Chunguang,Xia Jianfei,Zhang Feifei,Wang Zonghua
A three-dimensional (3D) self-assembled AuNPs/Ti 3 C 2 MXene hydrogel (AuNPs/Ti 3 C 2 MXH) nanocomposite was prepared for the fabrication of a novel microRNA-122 electrochemical biosensor. The 3D hydrogel structure was gelated from two-dimensional MXene nanosheets with the assistance of graphite oxide and ethylenediamine. MXene hydrogels supported the in situ formation of Au nanoparticles (AuNPs) that predominantly exploring the (111) facet, and these AuNPs are utilized as carriers for hairpin DNA (hpDNA) probes, facilitating DNA hybridization. MXene acted as both a reductant and stabilizer, significantly improving the electrochemical signal. In addition, the conjugation of PAMAM dendrimer-encapsulated AuNPs and H-DNA worked as an ideal bridge to connect targets and efficient electrochemical tags, providing a high amplification efficiency for the sensing of microRNA-122. A linear relationship between the peak currents and the logarithm of the concentrations of microRNA-122 from 1.0 × 10 −2 to 1.0 × 10 2 fM (I = 1.642 + 0.312 lg c , R 2 = 0.9891), is obtained. The detection limit is 0.8 × 10 −2 fM (S/N = 3). The average recovery for human serum detection ranged from 97.32 to 101.4% (RSD < 5%). Graphical Abstract
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Frontiers in Bioengineering and Biotechnology
·
2025
·
IF 4.8
Xiaojuan You,Mingyi Shao,Huadong Wang,Rui Zhu,Xinwei Liu,Lei Dong,Yuesheng Gong,Yongwei Li
Helicobacter pylori is one of the main causes of gastritis and gastric ulcer. Early detection of H. pylori is of great significance for the prevention of gastric cancer. Herein, a sensitive electrochemical aptasensor using AgNPs-GO as redox probes was established for the specific detection of H. pylori in blood serum and stool samples. Firstly, AgNPs-GO nanocomposites were obtained by in-situ reduction of AgNPs on graphene oxide (GO) surface with glucose as reducing agent, in which AgNPs showed good biocompatibility and chemical stability, as well as redox property, and GO provided a large surface area to assemble a large number of AgNPs. Subsequently, the electrodeposition of AuNPs further improved the conductivity of the aptasensor. Additionally, the streptavidin was introduced into the aptasensor to effectively bind the biotin-modified aptamers. In this way, aptamers could be tethered to the surface through SA-biotin linkage, and H. pylori was selectivly binded by the aptamers subsequently. Under optimal conditions, the aptasensor could detect H. pylori in a wide concentration range (101 CFU mL−1–108 CFU mL−1) with a low detection limit of 3 CFU mL−1. What’s more, the developed method showed excellent performance in practical application, which provided a promising possibility for the detection of other pathogens in clinical diagnosis.
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Advanced Healthcare Materials
·
2019
·Vol. 9
·期 1
·1901229
·
IF 6.27
Shi-Zhao Lu,Xiao-Yan Guo,Mei-Shuai Zou,Zi-Qin Zheng,Yu-Chuan Li,Xiao-Dong Li,Li-Li Li,Hao Wang
The emergence of drug-resistant bacteria is becoming the focus of global public health. Early-stage pathogen bioimaging will offer a unique perspective to obtain infection information in patients. A photoacoustic (PA) contrast agent based on functional peptide modified gold nanoparticles (AuNPs@P1) is developed. These nanoparticles can be specifically tailored surface peptides by bacterial overexpressed enzyme inducing in situ aggregation of the gold nanoparticles. In the meantime, the close aggregation based on the hydrogen bonding, π–π stacking, and hydrophobic interaction of the peptide residues on the surface of gold nanoparticles exhibits a typical redshifted and broadened plasmon band. In addition, this active targeting and following in situ stimuli-induced aggregation contribute to increased nanoparticle accumulation in the infected site. Finally, the dynamic aggregation of AuNPs@P1 results in dramatically enhanced photoacoustic signals for bioimaging bacterial infection in vivo with high sensitivity and specificity. It is envisioned that this PA contrast agent may provide a new approach for early detection of bacterial infection in vivo.
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LUMINESCENCE
·
2017
·Vol. 32
·期 7
·1277-1282
·
IF 1.509
Keying Cai,Mulan Zeng,Fenfen Liu,Nan Liu,Zhenzhong Huang,Yonghai Song,Li Wang
An easy and effective strategy for synthesizing a ratiometric fluorescent nanosensor has been demonstrated in this work. Novel fluorescent BSA–AuNPs@Tb–AMP (BSA, bovine serum albumin; AMP, adenosine 5′-monophosphate; AuNPs, Au nanoparticles) metal–organic framework (MOF) nanostructures were synthesized by encapsulating BSA–AuNPs into Tb–AMP MOFs for the detection of 2,6-pyridinedicarboxylic acid (DPA) and Hg 2+ . DPA could strongly co-ordinate with Tb 3+ to replace water molecules from the Tb 3+ center and accordingly enhanced the fluorescence of Tb–AMP MOFs. The fluorescence of BSA–AuNPs at 405 nm remained constant. While the fluorescence of BSA–AuNPs at 635 nm was quenched after Hg 2+ was added, the fluorescence of Tb–AMP MOFs remained constant. Accordingly, a ratiometric fluorescence nanosensor was constructed for detection of DPA and Hg 2+ . The ratiometric nanosensor exhibited good selectivity to DPA over other substances. The F 545 /F 405 linearly increased with increase of DPA concentration in the range of 50 nM to 10 μM with a detection limit as low as 17.4 nM. F 635 /F 405 increased linearly with increase of Hg 2+ concentration ranging from 50 nM to 1 μM with a detection limit as low as 20.9 nM. Additionally, the nanosensor could be successfully applied for the determination of DPA and Hg 2+ in running water.
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Analytical Methods
·
2016
·Vol. 8
·期 22
·4391-4397
·
IF 1.915
Juan Zhao,Wenjuan Guo,Meishan Pei,Feng Ding
In this work, a novel electrochemical aptasensor was developed for the sensitive detection of the antibiotic penicillin, which used a penicillin aptamer as the specific recognition element and a novel composite film consisting of a magnetic graphene nanocomposite (GR–Fe3O4NPs) and a poly(3,4-ethylenedioxythiophene)–gold nanoparticles composite (PEDOT–AuNPs) as the platform. Firstly, GR–Fe3O4NPs was fabricated on a bare glassy carbon electrode (GCE) surface. Secondly, the first film was modified with the PEDOT–AuNPs composite and the as-modified electrode had a much larger surface area and a greatly improved electronic transmission rate. Subsequently, a penicillin aptamer was assembled on the modified electrode via Au–N bonds between the AuNPs and –NH2 groups of the aptamer. The structures of the GR–Fe3O4NPs and PEDOT–AuNPs composite have been investigated by scanning electron microscopy (SEM). Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV) were used to demonstrate the electrochemical performance of the as-prepared aptasensor. Under optimized conditions, the proposed aptasensor exhibited high sensitivity for the detection of penicillin with a wide linear range from 0.1 to 200 ng mL−1 and a low detection limit of 0.057 ng mL−1. The proposed aptasensor also showed good stability, satisfactory reproducibility and high specificity. Finally, the proposed aptasensor was successfully used for the detection of penicillin in a real sample.
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高影响因子
Nanoscale
·
2017
·Vol. 9
·期 47
·18699-18710
·
IF 7.367
Bing Tan,Huimin Zhao,Weihao Wu,Xuan Liu,Yaobin Zhang,Xie Quan
Two-dimensional (2D) metal–organic framework (MOF) nanosheets emerging as a new member of the 2D family have received significant research interest in recent years. Herein, we have successfully synthesized 2D copper-based MOF nanosheets with bimetallic anchorage using a facile two-step process at room temperature and ambient pressure, denoted as Cu(HBTC)-1/Fe3O4-AuNPs nanosheets. The as-synthesized 2D bimetallic MOF nanosheets displayed enhanced peroxidase-like activity with relatively high catalytic velocity and affinity for substrates compared with previously reported peroxidase mimics. Furthermore, their intrinsic peroxidase-like catalytic activity could be flexibly regulated by single-stranded DNA (ssDNA), exhibiting the enhancement of 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation or inhibition of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)diammonium salt (ABTS) oxidation due to the adsorption of ssDNA via π–π stacking. Accordingly, on the basis of their peroxidase-like activity, our prepared 2D bimetallic immobilized MOF nanosheets achieved ultra-sensitive detection of H2O2 with a linear range of 2.86 to 71.43 nM, and comparable detection performance for glucose with a linear range of 12.86 to 257.14 μM. By means of their controllable peroxidase-like activity, a versatile colorimetric sensing platform was developed which realized the detection of sulfadimethoxine (SDM) with a linear range of 3.57 to 357.14 μg L−1 and the limit of detection (LOD) of 1.70 μg L−1. With the multiplexed performance for detecting various targets, our as-synthesized bimetallic MOF nanosheets hold great promise for applications in environmental monitoring, as well as bioassays by virtue of their good biocompatibility.
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JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2023
·Vol. 170
·期 4
·047509
·
IF 3.9
Mengli Wang,Lele Ma,Le Kang,Mingsan Miao,Huaixia Yang
Estrogen receptor alpha (ERα) is an important biomarker for breast cancer which is positive in over 75% of breast cancer cases. In this study, an electrochemical biosensor was constructed for sensitive detection of ERα based on the signal amplification of AuNPs-SWCNT nanocomposites and hemin@HKUST-1. AuNPs-SWCNT was served as a conductive substrate, and be used to immobilize the aptamer (Apt) by an Au–S bonding. Then, Apt, ERα and antibody (Ab) formed a sandwich composite structure through specific recognition and immobilized on the electrode. The hemin@HKUST-1 was introduced into the biosensor via an amide bond with Ab and acted as the signal molecule. AuNPs-SWCNT could significantly enhance the conductivity of the electrode and the large specific surface area of HKUST-1 enabled it to load more hemin, and greatly improved the sensitivity of the biosensor. The detection limit (LOD) of the biosensor could reach 27.8 fg·ml−1 after optimization of the conditions, with the current intensity and the logarithm of the ERα concentration exhibited a good linear relationship between 100 fg·ml−1 and 100 ng·ml−1. In addition, the biosensor possessed excellent selectivity, stability, reproducibility and Interference immunity. More importantly, it shows great application potential in practical detection.
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MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 3
·1-11
·
IF 5.3
Liu Huamin,Li Chenghong,Wang Lina,Fang Lichao,Huang Hui,Deng Jun,Hu Yue,Li Mimi,Ran Xiaoping,Li Lulu,Zheng Junsong
A “signal-off” photoelectrochemical (PEC) sensing platform has been designed for the ultrasensitive detection of DNA methylation levels and multiple methylated sites. The platform employs tungsten trioxide and TpPa-1-COF loaded by gold nanoparticle (AuNPs@WO 3 @TpPa-1-COF) composite material as the photoactive component and p-type reduced graphene (rGO) as an efficient quencher. The PEC signal of AuNPs@WO 3 @TpPa-1-COF composite is effectively quenched in the presence of p-type rGO, because p-type rGO can compete with AuNPs@WO 3 @TpPa-1-COF to deplete light energy and electron donors. In addition, a hybrid strand reaction (HCR) amplification strategy fixes more target DNA and then combines with rGO-modified anti-5-methylcytosine antibody to facilitate ultrasensitive DNA methylation detection. Under optimal conditions, DNA methylation can be measured within a linear concentration range of 10 –14 to 10 –8 M, with an exceptionally low detection limit of 0.19 fM (S/N = 3). At the same time, the platform can conduct quantitative determination of multi-site methylation, with the linear equation △I = 44.19Log A + 61.43, and the maximum number of methylation sites is 5. The sensor demonstrates high sensitivity, excellent selectivity, and satisfactory stability. Furthermore, the proposed signal-off PEC strategy was successfully employed to detect DNA methylation in spiked human serum samples, with recoveries ranging from 93.17 to 107.28% and relative standard deviation (RSD) ranging from 1.15 to 5.49%. Graphical
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