Advanced Science
·
2022
·Vol. 9
·期 13
·2105106
·
IF 17.521
Hao Su,Yongchun Liu,Yingtao Gao,Chengyu Fu,Chen Li,Rongrong Qin,Lei Liang,Peng Yang
Pesticide overuse is a major global problem and the cause of this problem is noticeable pesticide loss from undesired bouncing of sprayed pesticide droplets and rain erosion. This further becomes a primary source of soil and groundwater pollution. Herein, the authors report a method that can enhance pesticide droplet deposition and adhesion on superhydrophobic plant leave surfaces by amyloid-like aggregation of bovine serum albumin (BSA). Through the reduction of the disulfide bond of BSA by tris(2-carboxyethyl) phosphine hydrochloride (TCEP), the amyloid-like phase transition of BSA is triggered that rapidly affords abundant phase-transitioned BSA (PTB) oligomers to facilitate the invasion of the PTB droplet into the nanostructures on a leaf surface. Such easy penetration is further followed by a robust amyloid-mediated interfacial adhesion of PTB on leaf surface. As a result, after mixing with pesticides, the PTB system exhibits a remarkable pesticide adhesion capacity that is more than 10 times higher than conventional fixation of commercial pesticides. The practical farmland experiments show that the use of PTB aggregation could reduce the use of pesticides by 70–90% while ensuring yield. This work demonstrates that current pesticide dosage in actual agriculture production may be largely reduced by utilizing eco-friendly amyloid-like protein aggregation.
研究领域:
农业科学
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环境科学
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材料科学
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生物技术
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产品关联
DOI
PubMed
高影响因子
DESALINATION
·
2024
·
IF 8.3
Kun Wang,Zihao Ge,Mianliang Ji,Jian Li
Block copolymer-based porous materials have found extensive applications across a broad spectrum of industries. Their customized structures and scalable production capabilities have provided numerous possibilities to design membranes with precise separation properties. In this study, mPEGx-b-PAN block copolymers have been synthesized by incorporating mPEG of varying molecular weights in order to improve the selectivity and the antifouling properties of the resulting ultrafiltration membranes. Microphase separation within mPEG and PAN blocks was dependent on mPEG chain length and block concentration, ultimately leading to the controlled creation of pores in the mPEGx-b-PAN ultrafiltration membrane. Simultaneously, the surface layer thickness of the composite membrane was considerably reduced after adding the block copolymer. The optimized UF 2000 – 1.0 membrane, prepared by blending 1 % mPEG 2000 -b-PAN with PAN, exhibited a high permeability of 340.75 ± 22.17 L m −2 h −1 bar −1 and a BSA rejection rate of over 99 %, effectively separating organic pollutants such as HA, CR, and CB-G250. Furthermore, antifouling tests showed that the mPEG 2000 -b-PAN/PAN composite membranes exhibited a gradual degradation of permeability during the antifouling stage, with high permeability recovery after backwashing. Therefore, mPEGx-b-PAN block copolymers demonstrate promising potential for membrane separation applications and pave the way for significant advancements in filtration technology.
研究领域:
材料科学
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环境工程
·
化学工程
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产品关联
DOI
高影响因子
Advanced Biosystems
·
2018
·Vol. 2
·期 10
·1800115
·
IF 0
Jin Hong,Yu-Feng Chen,Yu Ma,Fen-Fen Zhang,Chun-Ming Wang,Ya Ding
To directly compare the surface modifier effects on the property regulation of nanoparticle (NP)-based systems, a fluorescence quenched nanoprobe (GpF) responsive to matrix metalloproteinases (MMPs) is employed as a demonstration. Polyethylene glycol (PEG) and bovine serum albumin (BSA) are modified on the surface of GpF via different modes, the S Au covalent bond and nonspecific interaction, respectively. PEG and BSA display similarly protective capability to GpF and increase their biocompatibility that are demonstrated in stability, cytotoxicity, and hemolysis studies. Although PEGylated NPs show better in vivo long circulation property than BSA-decorated ones, the merit of BSA in controlling the system size in a relatively small range improves the penetration of GpF-BSA in tumor tissues and reduces the accumulation in normal tissues. Through an MMP-dominated catalysis and then a glutathione-assisted replacement process, the fluorescence recovery of GpF-BSA shows higher efficiency than PEG-modified NPs. It can be explained by the low steric hindrance and exchangeable manner of BSA on the surface of GpF. The results in this work demonstrate that an endogenous protein having dysopsonization function will be a promising material for the construction and surface modification of nanosystems for biomedical applications.
研究领域:
生物医学工程
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纳米医学
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分子生物学
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产品关联
DOI
DESALINATION
·
2022
·
IF 11.211
Wenxin Zhang,Yaqi Zhang,Yuze Wang,Shiwei Tian,Na Han,Wei Li,Wei Wang,Hongwei Liu,Xuhuan Yan,Xingxiang Zhang
Membrane fouling remains a significant restriction for the long-time operation. In this paper, fluffy-like amphiphilic graphene oxide (f-GO) was prepared by simple grafting silane coupling agent (KH-570) onto the graphene oxide matrix, and thus featured with good dispersion behavior in organic solution. The f-GO was further incorporated into the PAN-based (poly(acrylonitrile-methyl acrylate), P(AN-MA)) copolymer during the phase inversion process. Compared with the P(AN-MA) membrane, the resistance of the f-GO/P(AN-MA) composite membrane reduced from 4.49 × 10 −12 m −1 to 1.78 × 10 −12 m −1 . The pure water flux increased from 200 to 326 L/m 2 h, while the water contact angle declined from 92.8° to 68.3°. It is worthy to note that the fouling mode changed from irreversible fouling to reversible fouling , with the total fouling rate showing a half decay. The fouling recovery rate reached 98.16% and the BSA rejection rate increased from 70.59% to 99.33% as 0.5 wt% f-GO was incorporated. The f-GO is easily scaled up and can be widely applied in other polymer matrices , showing potential application prospects in the membrane separation and purification area. The f-GO/P(AN-MA) composite membranes with excellent water flux, BSA rejection and FRR, are candidates for further employment in wastewater purification applications.
研究领域:
材料科学
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环境工程
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产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 17
·3564-3569
·
IF 1.915
Chunzhi Xuan,Qiao Xia,Juanjuan Xu,Qinghong Wang,Xia Lin,Yingzi Fu
The nanocomposite (Au@BSA) which was synthesized using gold nanoparticles (AuNPs) and bovine serum albumin (BSA) was used as an electrochemical sensing layer for chiral recognition of propranolol (PRO). Scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), ultraviolet-visible (UV-vis) spectroscopy and amperometric testing technique were employed to characterize the Au@BSA nanocomposite. Differential pulse voltammetry (DPV) and atomic force microscopy (AFM) were used to assess the enantioselective performance of the fabricated interface, and the optimal experimental conditions, such as acidity and incubation time were also discussed. The results showed that after the modified interface interacted with PRO enantiomers larger discrepancy was obtained from S-PRO, and linear electrochemical responses to PRO enantiomers were obtained from 1.0 × 10−5 to 5.0 × 10−3 mol L−1 with a detection limit of 3.3 × 10−6 mol L−1 (S/N = 3). To gain more information on the role of gold nanoparticles (AuNPs) and bovine serum albumin (BSA) in the chiral recognition of PRO enantiomers, the interface fabricated using AuNPs or BSA was also allowed to interact with PRO enantiomers, and no obvious signal discrepancy was obtained. The results suggested that only Au@BSA could be employed for the enantioselective recognition of PRO enantiomers.
研究领域:
分析化学
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生物传感
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材料科学
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DOI
高影响因子
LANGMUIR
·
2022
·
IF 4.331
Xiaolei Zhang,Bin Liu,Shu Yao,Zongming Liu,Jinkai Li
Photothermal therapy (PTT) has attracted great attention as an anticancer treatment strategy. With the rapid development of nanomedicine, multifunctional inorganic nanophotothermal agents provide a new way to improve the effect of PTT. Herein, bovine serum albumin (BSA)-modified Bi nanosheets (Bi NSs) with good biocompatibility were synthesized by a facile redox and ball milling method and applied as a photothermal agent for the enhancement of PTT. Owing to the strong near-infrared absorption, Bi NSs exhibit high photothermal conversion efficiency (η = 36.17%) under 808 nm laser irradiation and can serve as a nanotherapeutic agent for cancer therapy. In addition, in vitro cell safety analysis also suggests that the toxicity of BSA-modified Bi NSs is negligible. Upon 808 nm irradiation, the uptake ability of tumor cells to Bi NSs@BSA has been improved. Moreover, Bi NSs@BSA also can be used as a good contrast agent for CT imaging and then to observe the distribution of materials in the tumor site. Finally, Bi NSs@BSA-mediated PTT results show a high ablation rate of A549 tumor cells both in vitro and in vivo. All results reveal that Bi NSs@BSA is a promising nanotherapeutic platform for PTT.
研究领域:
纳米医学
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癌症治疗
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生物材料
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DOI
PubMed
高影响因子
RSC Advances
·
2015
·Vol. 5
·期 51
·40514-40520
·
IF 3.84
Na Chen,Weitao Yang,Yun Bao,Hualin Xu,Songbing Qin,Yu Tu
Radiation therapy has shown encouraging treatment efficacy on many types of tumors. However, the side effects from accumulated high dosage during radiation therapy hindered its wide application in some cases. In this study, BSA capped Au nanoparticles (BSA-AuNPs) are employed as radiation sensitizers, aimed at achieving high efficiency for tumor radiation therapy. Our results showed that BSA-AuNPs with a uniform size of ∼18 nm can reduce colony formation, and increase the DNA double-strand breaks (DSBs) at 0.5 and 2 h post-irradiation compared with treatment by radiation therapy alone in a U87 glioblastoma cell line. Cell apoptosis at 48 h is enhanced after radiation treatment with BSA-AuNPs as the sensitizers. An enhanced tumor regression is also observed on the group with BSA-AuNPs sensitized radiation therapy. No obvious toxicity is observed for in vitro and in vivo experiments. BSA-AuNPs show potential in tumor therapy as radiosensitizing agents.
研究领域:
癌症治疗
·
纳米技术
·
放射生物学
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DOI
高影响因子
Journal of Materials Chemistry B
·
2024
·
IF 6.1
Qijun Du,Ho-Yin Yuen,Jingke Pan,Chenwei Sun,Di Wu,Jie Liu,Guohua Wu,Xin Zhao,Shuqi Wang
The hypoxic microenvironment of solid tumors can lead to reduced therapeutic DNA damage to the tumor cells, thus diminishing tumor sensitivity to radiotherapy. Although hypoxic radiosensitizers can improve radiotherapy efficacy by enhancing the role of oxygen, their effects are limited by the uneven distribution of oxygen within solid tumor tissues. In this study, a novel radiosensitizer via leveraging gold complexes and metronidazole (MN) was synthesized to improve radiotherapeutic efficacy. The gold atoms incorporated in the radiosensitizer enabled efficient deposition of high-energy radiation; the hydrophobic metronidazole was reduced to hydrophilic aminoimidazole under hypoxia conditions and further promoted radiotherapy sensitization. The results of CCK-8 assays, Live/Dead assays, γ-H2AX immunofluorescence indicated that metronidazole-modified Au@BSA nanocomposites (NCs) exhibited excellent antitumor effects. The in vivo antitumor tests further showed an inhibition rate of 100%. These results demonstrated that the NCs successfully enhanced radiotherapy efficacy by the dual sensitization strategy. Overall, we believe this multimodal radiosensitizing nanocomplex can significantly inhibit tumor growth and metastasis, with their hypoxia-oriented characteristics ensuring a higher efficacy and safety.
研究领域:
癌症研究
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纳米技术
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放射肿瘤学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
Supramolecular Materials
·
2026
·
IF 8.2
Baichen Liu,Kai Wang,Ying Guan,Yongjun Zhang
Functional monomers play a key role in protein-imprinted polymers. Inspired by the ability of albumin to bind and deliver lipids, imidazolium-based ionic liquid (IL) monomers with an allyl group and a long chain alkyl group were selected as cationic functional monomer for synthesis of molecularly imprinted polymers (MIPs) of BSA. The “shape-memorable imprint cavity” strategy, which uses peptide crosslinkers (PCs) to construct shape-memorable imprint cavities, was also adopted. Fluorescence quenching demonstrated that the IL monomers have a larger binding constant with BSA than the conventional cationic monomer DMAEMA, because they bind with BSA via not only electrostatic interaction but also hydrophobic force due to their long-chain alkyl group. In addition, with increasing length of the alkyl group, the IL monomers bind with BSA more strongly. Compared with the BSA MIP using DMAEMA, significantly improved imprinting efficiency was observed when using the IL monomers as cationic monomers. Particularly the MIP using [C 8 VIm]Br exhibits an adsorption capacity of 983.90 mg/g and an imprinting factor of 7.76. The selectivity of the MIP towards BSA was also improved. In addition, like other MIPs using the “shape-memorable imprint cavity” strategy, the new BSA MIPs also exhibit advantages such as complete template removal under mild conditions and excellent reusability. The new BSA MIP was successfully used to extract BSA from fetal bovine serum, demonstrating its high potential for the separation and purification of BSA from complicated biological fluids.
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9
Benyu Sun,Ying Guan,Yongjun Zhang
It still remains a big challenge to separate a target protein from a complicated biological samples selectively. Here BSA-imprinted silica nanoparticles were synthesized by combining several new strategies. Firstly, a novel surface enzyme-initiated polymerization was used to synthesize the imprinted polymer shell over nanoparticles. Particle agglomeration, a key problem for conventional surface imprinting, was successfully avoided, even when high concentration prepolymerization solutions were used. Therefore high imprinting efficiency was achieved because of the enhanced pre-assembly between the template and functional monomers. Secondly, the “shape-memory imprint cavity” strategy was used in which a peptide crosslinker capable of undergoing pH-induced helix-coil transition was used as crosslinker. This strategy not only facilitate the removal of template protein, but also significantly improve imprinting efficiency. In addition, to rebind protein at physiological pH, a new peptide crosslinker which adopts an α-helical conformation at physiological pH but converts to extended coil conformation at high pHs was used. The resulting BSA-imprinted silica nanoparticles exhibited high adsorption capacity (119.7 mg g −1 ), high imprinting factor (8.86), high selectivity, good reusability, and rapid binding kinetics. The particles can separate BSA from mixed protein solutions and even complex serum samples selectively, efficiently and quickly. More importantly, because the process was performed under very mild conditions, i.e., at room temperature and in aqueous solution with physiological pH, the biological activity of the remaining proteins was not affected.
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产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Quan-Quan Zhuang,Shao-Bin He,Kai-Yuan Huang,Hua-Ping Peng,Chang-Mai Chen,Hao-Hua Deng,Xing-Hua Xia,Wei Chen,Guo-Lin Hong
Various types of bovine serum albumin (BSA)-protected fluorescent gold nanoclusters (BSA-AuNCs) have been fabricated and applied in various fields. However, the conventional synthesis methods for BSA-AuNCs usually yield a low photoluminescence quantum yield (PLQY) in solution. In this study, we systematically examined the influences of incubation time, temperature, and pH on the formation process of BSA-AuNCs and then developed a novel strategy to synthesize BSA-AuNCs with PLQY (26%), far exceeding that of existing counterparts. Of the three important factors, pH, temperature, and time, pH plays a key role in the formation of BSA-AuNCs with different compositions and fluorescence properties. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) results showed that BSA-Au 20 NCs with high purity can be produced at a pH value of 10 and the correct combination of incubation temperature and reaction time. The advantages of the obtained BSA-Au 20 NCs, including small size, high PLQY, long lifetime, high purity, as well as facile modification, make them ideal candidates for luminescent probes in imaging and sensing applications.
研究领域:
生物化学
·
纳米科技
·
材料科学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 5
·期 2
·269-278
·
IF 4.872
Xiaodan Jia,Jing Bai,Zhifang Ma,Xiue Jiang
In this study, we present the synthesis and application of a new therapeutic platform based on photosensitizer-loaded, two-dimensional layered nanomaterials of WSe2 for combined photothermal and photodynamic cancer therapy. WSe2 nanosheets are synthesized by a simple and efficient chemical exfoliation using bovine serum albumin (BSA) as an effective exfoliating agent and strong stabilizing agent, endowing the WSe2–BSA nanosheets obtained with high stability in physiological solutions and good biocompatibility without further functionalization. The WSe2–BSA nanosheets can be employed not only as an efficient photothermal agent but also as a carrier to load methylene blue (MB) as a photosensitizing agent for photodynamic therapy. Moreover, the generation of singlet oxygen can be regulated by controlling the release of MB from the WSe2 nanosheets with NIR irradiation. The therapeutic efficacy of the MB-loaded WSe2–BSA (WSe2–BSA/MB) complexes was evaluated in vitro and in vivo. It was found that dual-mode photothermal and photodynamic therapy has a significant synergistic effect and dramatically improves the therapeutic efficacy toward cancer cells compared with single mode treatment. These findings indicate that WSe2 nanosheets could be a promising therapeutic agent and smart carrier for the synergistic combination of phototherapy towards biomedical applications.
研究领域:
材料科学
·
生物医学工程
·
癌症治疗
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）