FOOD CHEMISTRY
·
2022
·
IF 9.231
Yu-Zhu Zhu,Kai Chen,Yu-Lin Chen,Changjun Zhang,Yuan-Yuan Xie,Robert C. Hider,Tao Zhou
In order to develop the tyrosinase inhibitors with potential application in food industry, a series of stilbene-hydroxypyridinone hybrids were prepared. Among these compounds, 1h was found to possess the most potent tyrosinase inhibitory effect on both monophenolase and diphenolase activities, with IC 50 values of 2.72 μM and 15.86 μM, respectively. The inhibitory effect of 1h on monophenolase activity was 4.6 times that of kojic acid . An inhibition kinetic assay indicated that 1h was a mixed-type and reversible inhibitor. The copper-binding and reducing ability assays, molecular docking study, intrinsic and ANS–binding fluorescence assays indicated that copper coordination and reduction is likely to be the causative mechanism for 1h -induced inhibition on tyrosinase. The results of color measurement and browning index determination indicated that treatment with 1h retarded effectively the browning of freshly-cut apples during their storage. Meanwhile, PPO and POD activities in apple slices were found to be effectively inhibited.
研究领域:
食品科学
·
生物化学
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DOI
高影响因子
Foods
·
2024
·Vol. 13
·期 20
·3295
·
IF 4.7
Kok Bing Tan,Meixia Zheng,Junyan Lin,Yujing Zhu,Guowu Zhan,Jianfu Chen
Loquat leaf extract (LLE) was added to guar gum and pullulan as an environmentally friendly packaging film (GPE) to preserve Chinese water chestnuts (CWCs). The effect of the amount of LLE on the guar gum/pullulan composite film was investigated. The optimal amount of LLE was 4% (GPE4), with lower water vapor permeability (WVP) and greater mechanical strength, antioxidant, and comparable antibacterial performance than many pullulan-based films. Upon packing the CWCs for 4 days, the weight loss rate of GPE4 was only 1.80 ± 0.05%. For GPE4, the POD activity, the soluble solid content, and the vitamin C (Vc) content of the CWCs were 21.61%, 36.16%, and 26.22% higher than those of the control sample, respectively. More importantly, GPE4 was effective in preserving the quality of CWCs after 4 days of storage, better or at least comparable to non-biodegradable plastic wrapping (PE). Therefore, it can be concluded that GPE films hold significant promise as a sustainable alternative packaging material for preserving fruit-based foods like CWCs, potentially replacing PE in the future.
研究领域:
食品科学
·
包装工程
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植物化学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2026
·
IF 4.9
Shi Minke,Wang Jiarui,Guo Qihan,Wang Jiayu,Sarengaowa,Ji Yaru,Xiao Ying,Feng Ke
Stem lettuce is a nutritious vegetable rich in dietary fiber, vitamins, and bioactive compounds, but fresh-cut processing accelerates browning, oxidative stress, and quality deterioration during storage. This study investigated the effects of ethanol vapor on browning inhibition, antioxidant capacity, and reactive oxygen species (ROS) metabolism in fresh-cut stem lettuce. Samples were treated with ethanol vapor at 0, 0.5, 1.0, and 2.0 mL/L and stored for 12 days. Among the treatments, 0.5 mL/L ethanol vapor most effectively delayed browning by suppressing the activities of peroxidase, polyphenol oxidase, and phenylalanine ammonia-lyase, while reducing the accumulation of superoxide anion radicals and hydrogen peroxide. This treatment also enhanced antioxidant defense systems by increasing the activities of superoxide dismutase, catalase, ascorbate peroxidase, and glutathione reductase, as well as non-enzymatic antioxidant capacity, as indicated by elevated FRAP, DPPH, and ABTS scavenging activities. By the end of storage, FRAP and DPPH values in the 0.5 mL/L group were approximately 1.8 and 2-fold higher than those of the control. Overall, ethanol vapor at 0.5 mL/L effectively alleviated oxidative stress, maintained ROS homeostasis, and delayed enzymatic browning, demonstrating its potential as a practical preservation strategy to improve postharvest quality and extend the shelf life of fresh-cut stem lettuce during cold storage.
✓ 已验证
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2025
·
IF 8
Lingling Liu,Yueyue Zhang,Linxuan Jin,Mehdi Abdollahi,Guoliang Zhao,Karthikeyan Venkatachalam,Zhaojun Ban
Although plant essential oils are widely recognized for their synergistic antimicrobial effects in postharvest fruit preservation, their use in food preservation coatings is limited due to their low water solubility and high volatility. To address these limitations, a self-assembling material SPI/SA/GSH (SSG) was developed by introducing glutathione (GSH) into a soy protein isolate (SPI) - sodium alginate (SA). The cinnamon essential oil (CEO) was encapsulated within this self-assembling material using homogenization and sonication techniques, to enhance its preservation capabilities. The solutions and emulsions composed of SPI, SA, SPI/SA physical complex (SS) and SSG particles were compared and evaluated for their structural characteristics and stability, indicating that the introduction of GSH promoted the self-assembly of protein-polysaccharide complexes by breaking disulfide bonds, thereby improving the emulsification and stability of the system. The antibacterial assays showed that the SSG emulsion effectively inhibited the growth of Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). Notably, the SSG displayed a sustained-release effect for CEO, highlighting its potential for controlled-release applications. Furthermore, fresh-cut cantaloupe treated with the SSG coating experienced reduced mass loss and retained firmness, while maintaining levels of ascorbic acid, total soluble solids (TSS), and titratable acidity (TA) compared to other treatments. These findings underscore the potential of SSG for improving food preservation and extending shelf life.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Kok Bing Tan,Meixia Zheng,Junyan Lin,Yujing Zhu,Guowu Zhan,Jianfu Chen
Pullulan-based composite film can be a potential alternative packing material to non-environmentally friendly plastic wrap (PE) to preserve fresh-cut carrots. However, many developed pullulan-based composites either have high water vapor permeability (WVP) and high mechanical strength or vice versa, which limits the practicality of the developed packaging materials for potential commercialization. Herein, Abelmoschus manihot gum (AMG)/pullulan/magnesium L-ascorbate (MLA) was created as a green composite film (APL) to preserve fresh-cut carrots. The optimal amount of MLA was found to be 10 % (APL10), demonstrating a balance of lower WVP and greater mechanical strength and antioxidant performance than many pullulan-based films. This effectively solved many problems faced by other pullulan-based packaging films. After the fresh-cut carrots were packed with the composite film for 4 days, it was found that APL10 was effective in preserving the quality of carrots, in terms of freshness, weight loss rate, Vitamin C (VC), and malondialdehyde (MDA) content after 4 days of storage, much better than non-biodegradable PE. Thus, based on these findings, it is concluded that APL films have huge potential as a green packaging material for food to replace PE in the future.
研究领域:
食品科学
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2024
·
IF 3.9
Xinping Wu,Qiuying Li,Yang Yi,Limei Wang,Wenfu Hou,Youwei Ai,Hongxun Wang,Ting Min
The quality of fresh-cut Chinese water chestnuts (CWCs) rapidly deteriorates with prolonged storage time, thus affecting its commercial value. This study aimed to investigate the effect of cinnamaldehyde (CA) treatment on the quality of fresh-cut CWCs and its mechanism of action. CA treatment maintained good appearance and higher total soluble solids content (TSS) of fresh-cut CWCs and also significantly inhibited microbial proliferation, respiration, phenylalanine ammonia-lyase activity (PAL) and enzymatic browning substance content. Moreover, CA treatment increased the antioxidant capacity and defense function-related enzyme activities (PPO and POD) of fresh-cut CWCs, and suppressed ·OH production rate and MDA content. Meanwhile, CA treatment significantly increased the content of antioxidant compounds and enzyme activities of the AsA-GSH cycle system. In conclusion, CA can inhibit surface discoloration of fresh-cut CWCs and extend their shelf life, thus increasing their commercial value.
研究领域:
食品科学
·
采后生理学
·
植物生物化学
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2025
·
IF 2.8
Yao Ma,Qing-nan Chen,Rui Liao,Yun-e Bai,Jian-kuan Li,Shuang Hu,Jian-ping Gao
In this study, a cyclodextrin-dispersive liquid-liquid microextraction-HPLC-based method was developed to quantitatively study four components of Codonopsis Radix (CR): carboxymethyl- β -cyclodextrin (CM- β -CD) was incorporated into the DES, then added to the sample solution, and the mixture was vortexed and centrifuged, the upper hydrophobic phase was then collected for HPLC analysis. The primary elements influencing the extraction efficiency were optimized to choose the circumstances for this experiment, including the type and consumption of cyclodextrin, the NaCl concentration, the pH level of the sample phase and the extraction time. The EFs for four target components extracted by cyclodextrin-DES were between 8.4 and 71.2, which were all higher than those extracted only by DES. In their respective linearity ranges, the four analytes exhibited good linearity (R 2 ≥ 0.99), with detection limits of 1.2 × 10 −2 μg/mL, 5.3 × 10 −3 μg/mL, 3 × 10 −4 μg/mL, and 1.1 × 10 −4 μg/mL. The precision and accuracy of the method ranged from 0.8 % to 10.0 % and 90.0 % to 105.2 %. Finally, this method was applied to the quantification of fresh-cut CR and traditional-processed CR. The results showed that the content of tangshenoside I and lobetyolinin of fresh-cut CR was significantly higher than in traditionally processed CR, but there were no significant differences between fresh-cut CR and traditionally processed CR for lobetyolin and lobetyol. The use of CM- β -CD in combination with DES provides rapid, simple, and reliable quantification of the target analytes. By this method, differences between the two processing methods were compared at the level of small-molecule content, providing evidence for the feasibility of the fresh-cutting processing method and demonstrating a theoretical basis for the quality evaluation method of the fresh-cut process.
研究领域:
分析化学
·
植物化学
·
天然产物
·
分离科学
·
中药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
·
2024
·
IF 2.3
Wei-Ming Chai,Qiuhan Bai,Qiuxia Pan,Linjun Wang,Du Zhu
6,7-Bis-(2-methoxyethoxy)-4(3 H )-quinazolinone (BMEQ) was selected from quinazolinones for its strong tyrosinase inhibitory activity ( IC 50 = 160 ± 6 μM). It suppressed tyrosinase activity in a competitive way and quenched the fluorescence of the enzyme through a static mechanism. The binding of BMEQ to tyrosinase increased the hydrophobicity of the latter and facilitated non-radiative energy transfer between them. The formation of BMEQ–tyrosinase complex was driven by hydrogen bonds and hydrophobic interactions, and it loosened the basic framework structure of tyrosinase, affecting the conformation of the enzyme, and leading to a decrease in tyrosinase activity. In addition, the BMEQ postponed the oxidation of phenolics and flavonoids by inhibiting polyphenol oxidase (PPO) and peroxidase (POD), which resulted in the inhibition of the browning of fresh-cut apples. This study identified a novel tyrosinase inhibitor BMEQ and verified its potential application for improving the preservation of postharvest fruits.
研究领域:
食品科学
·
植物生理学
·
酶学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Le Zhang,Enlin Han,Yulun Wu,Xiaodong Wang,Dezhen Wu
The surface decoration of short-cut polyimide (PI) fibers with multi-walled carbon nanotubes (MWCNTs) was performed by fabricating a polydopamine (PDA) coating layer on the fiber surface and then immobilizing MWCNTs onto the coating layer via covalent bonding. This successful surface decoration was confirmed by scanning electron microscopy, X -ray photoelectron spectroscopy, Fourier -transform infrared microscopy and static water contact angle. The application of the surface-decorated PI fibers as reinforcing fibers for reinforcement of polycarbonate (PC)/acrylonitrile–butadiene–styrene copolymer (ABS) alloy was investigated, which indicated that the MWCNTs-decorated PI fibers not only could effectively reinforce the PC/ABS alloy but also generated a significant lightweighting effect on the resulting composites. The maximum mechanical properties were achieved for the composites at a fiber content of 20 wt.% and a fiber length of 3 mm. This significant reinforcement effect is attributed to the enhancement of interaction bonding strength between the fibers and matrix as a result of the surface decoration of PI fibers with MWCNTs. The morphological investigation suggested that fiber rupture was the major energy dissipation mechanism in the tensile and impact failures, whereas fiber debonding and pullout were partly involved in the fracture energy dissipation. In addition, the presence of surface-decorated PI fibers slightly enhanced the thermal stability and load bearing capability of composites. This work can provide a type of high-performance lightweight composite material for automobile and aviation industries.
研究领域:
材料科学
·
复合材料工程
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2021
·Vol. 28
·期 24
·31470-31478
·
IF 4.223
Huang Ting,Tang Xiaomin,Zhang Shixin,Wang Wei,Zhang Jie,Tan Xuemei,Zheng Huaili
Refractory organic pollutants in tail water of dyeing wastewater treatment have aroused wide concern. Their efficient and cost-effective removal reduced their threat to public health and ecosystem. Herein, a novel short-cut photocatalysis/algal degradation-based hybrid technology was implemented in efficient removal of methylene blue (MB) in simulated tail water using reliable titanium dioxide and common Chlorella pyrenoidosa , and the mechanisms in processes were emphasized. The treatment efficiency was significantly improved via pretreatment before chemical and biological degradation. MB of 79.71% was concentrated as the adsorption of the modified titanium dioxide and the collection of titanium dioxide by inorganic coagulant. The supernatant with low concentration of MB after coagulation was able to be directly treated by Chlorella pyrenoidosa . MB of 93.7% was degraded and transformed to intermediates in short-cut photocatalysis under visible light in 1 h. The intermediates owning the low biological inhibition were easily further degraded by Chlorella pyrenoidosa in 6 days. Mechanism analysis implied that the modified titanium dioxide was not simple monolayer adsorption, and physical adsorption was dominant. The coagulant played an essential role of charge neutralization in collection of the modified titanium dioxide. The removal of photocatalytic intermediates was divided to fast adsorption of Chlorella pyrenoidosa , low desorption in lag period of algae, and gradual biodegradation that accompanied with the increase of algal cell quantity.
研究领域:
环境科学
·
生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYST
·
2016
·Vol. 141
·期 20
·5864-5869
·
IF 4.033
Ningning Zhou,Guowen Meng,Zhulin Huang,Yan Ke,Qitao Zhou,Xiaoye Hu
This report presents a simple and inexpensive fabrication approach to a flexible transparent composite film as a “cut-and-paste” surface-enhanced Raman scattering (SERS) substrate for in situ detection of organic pollutants. First, a self-assembled monolayer of Ag-nanocubes (Ag-NCs) is obtained at the air/water interface. Then, the Ag-NC monolayer is retrieved onto a flexible transparent polyethylene (PE) film to achieve an Ag-NC@PE composite film as a flexible SERS substrate. As the Ag-NCs in the monolayer are closely and uniformly packed on the PE film, the Ag-NC@PE composite film shows high SERS-activity with good signal homogeneity and reproducibility. Furthermore, the flexible transparent Ag-NC@PE composite film is “cut into” small pieces and directly “pasted” onto contaminated fruits for in situ SERS detection, as a result 10 nM thiram, 1 μM 4-polychlorinated biphenyl and 10 nM methyl parathion contaminants on oranges are detected, respectively. Therefore the Ag-NC@PE composite film is an inexpensive and effective SERS substrate for rapid in situ detection of organic pollutants in aqueous solutions, on fruits and other solid objects.
研究领域:
材料科学
·
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Le-Le Shao,Xiao-Ling Wang,Kai Chen,Xiao-Wu Dong,Li-Min Kong,De-Yin Zhao,Robert C. Hider,Tao Zhou
A range of hydroxypyridinone derivatives were synthesized starting from kojic acid. Among them, 10 and 11 were found to possess the strongest inhibitory effect on monophenolase activity of mushroom tyrosinase, having IC 50 values of 2.04 and 1.60 μM, respectively. The IC 50 values of 10 and 11 for the inhibition of diphenolase activity of mushroom tyrosinase were determined as 13.89 and 7.99 μM, respectively. Investigation of the inhibitory mechanism of these two compounds indicated that the inhibition was reversible and of a competitive-uncompetitive mixed type. The K I and K IS values of 10 were determined to be 24.84 and 32.54 μM, respectively, and the corresponding values for 11 being 18.07 and 21.34 μM, respectively. The effect of 11 on the browning process of fresh-cut apples was evaluated by measuring the color change and browning index. The results indicated that 11 had a significant effect on controlling the browning of fresh-cut apple slices.
研究领域:
食品科学
·
生物学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）