Scientific Reports
·
2021
·Vol. 11
·期 1
·1-9
·
IF 4.38
Dong Wenjing,Song Erqun,Song Yang
The acute liver injury (ALI) and hepatic fibrosis caused by the co-treatment of lipopolysaccharide (LPS)/ d -galactosamine (D-GalN) have been extensively studied. However, whether LPS/D-GalN are genotoxic has been left unknown. In this study, male mice were divided into eight groups with eight animals in each group. For acute challenge of LPS/D-GalN, the mice in each group received a combination of LPS/D-GalN via intraperitoneal injection at the dose of 25 μg/kg/250 mg/kg, 25 μg/kg/500 mg/kg, or 50 μg/kg/500 mg/kg body weight. An additional group for chronic administration of test compounds was conducted by i.p. injection of LPS/D-GalN (10 μg/kg/100 mg/kg) every other day for 8 weeks. Saline solution (0.9%) and cyclophosphamide (CTX) (50 mg/kg body weight) given by i.p. injection was used as the negative and positive control, respectively. The results of single cell gel electrophoresis (SCGE) assay indicated that acute exposure of the mice to LPS/D-GalN caused severe DNA damage in hepatic cells, but not in the brain, sperm or bone marrow cells, which evidenced the genotoxicity of LPS/D-GalN administrated in combination. Interestingly, the chronic administration of LPS/D-GalN triggered significant genotoxic effects not only in hepatic but also in brain cells, with negative results in sperm and bone marrow cells. Histopathological examination in the liver and brain tissues revealed changes consistent with the SCGE results. The present study indicates genotoxic potential of LPS/D-GalN co-administered in mice, which may serve as an in vivo experimental model for relevant genotoxic study.
研究领域:
毒理学
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分子生物学
·
医学
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DOI
PubMed
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 5
·1181
·
IF 4.2
Minghe Wang,Zhouyuji Liao,Dongting Zhangsun,Yong Wu,Sulan Luo
Antimicrobial peptides (AMPs), acknowledged as host defense peptides, constitute a category of predominant cationic peptides prevalent in diverse life forms. This study explored the antibacterial activity of α-conotoxin RgIA, and to enhance its stability and efficacy, D-amino acid substitution was employed, resulting in the synthesis of nine RgIA mutant analogs. Results revealed that several modified RgIA mutants displayed inhibitory efficacy against various pathogenic bacteria and fungi, includingCandida tropicalisandEscherichia coli. Mechanistic investigations elucidated that these polypeptides achieved antibacterial effects through the disruption of bacterial cell membranes. The study further assessed the designed peptides’ hemolytic activity, cytotoxicity, and safety. Mutants with antibacterial activity exhibited lower hemolytic activity and cytotoxicity, with Pep 8 demonstrating favorable safety in mice. RgIA mutants incorporating D-amino acids exhibited notable stability and adaptability, sustaining antibacterial properties across diverse environmental conditions. This research underscores the potential of the peptide to advance innovative oral antibiotics, offering a novel approach to address bacterial infections.
研究领域:
药理学
·
微生物学
·
生物化学
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DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2023
·Vol. 39
·期 6
·976-984
·
IF 3.1
Hou Huipeng,Tang Shanshan,Wang Wei,Liu Miao,Liang Axin,Xie Bingteng,Yi Yue,Luo Aiqin
I n this work, an electrochemical chiral sensor of a nanowire-like chiral metal-organic framework/multiwalled carbon nanotube-chitosan/glassy carbon electrode( D -MOF/MWCNTs-CS/GCE) was proposed for the enantiorecognition of L / D -tryptophan( L / D -Trp). The asymmetrical spatial structure of D -MOF provides the feasibility for the enantiorecognition of Trp enantiomers. Moreover, differential pulse voltammetry(DPV) was carried out to be the detection method and the DPV peak potential difference(Δ E p ) between L -Trp and D -Trp was referred as the index of the enantiorecognition performance. Several parameters, such as mass ratios and drop-coated volume of MWCNTs-CS, drop-coated volume and concentration of D -MOF, pH and detection temperature of D -MOF/MWCNTs-CS/GCE were optimized for the largest Δ E p value. And the molecular dynamics(MD) simulation was used to elucidate the enantiorecognition mechanism. Furthermore, the proportions of D -Trp( D -Trp%) in Trp mixtures were detected in a good linear relationship with the DPV peak potentials( E p ), and the proposed electrochemical chiral sensor exhibited good reproducibility, stability and enantiorecognition ability. Additionally, the proposed electrochemical chiral sensor( D -MOF/MWCNTs-CS/GCE) has a good application prospect in the fields of biomedicine, clinical diagnosis, chemical production, pharmaceuticals safety and so on.
研究领域:
分析化学
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生物医学
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电化学
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DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
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2017
·
IF 3.648
Yonghong Hu,Yemin Yu,Yuhao Zhang,Wenge Yang,Tao Li,Renlun Deng,Min Jiang
The solid-liquid equilibrium (SLE) of d -sorbitol in (ethanol + acetonitrile) binary solvent mixtures was explored at temperatures ranging from 278.15 K to 333.15 K under 0.1 MPa. For the temperature range investigated, the equilibrium solubility varies with the composition of the solvent and temperature. The equilibrium solubility data were then modelled using modified Apelblat equation, a variant of the combined nearly ideal binary solvent/Redich-Kister (GSM) model and Jouyban-Acree model. Computational results showed that the modified Apelblat equation and CNIBS/R-K model have the low mean deviation ( MD ). In addition, the thermodynamic properties of the solution process, including the Gibbs energy , enthalpy, and entropy were calculated by the van't Hoff analysis.
研究领域:
化学工程
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物理化学
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DOI
高影响因子
JOURNAL OF ORGANIC CHEMISTRY
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2018
·
IF 4.805
Zhen-Hua Li,Zhi-Jiang Jiang,Qiao-Ling Shao,Jin-Jing Qin,Qiang-Feng Shu,Wen-Hao Lu,Wei-Ke Su
A one-pot cascade heterocyclization strategy has been developed for the synthesis of 2,4-dichloro-substituted pyrano[2,3-d]pyrimidines and furo[2,3-d]pyrimidines from linear γ- and β-ketomalononitriles using triphosgene and triphenylphosphine oxide. The reaction afforded synthetic useful products with moderate to good yields, bypassing the conventional harsh conditions of chlorination. The mechanistic study revealed that the reaction proceeded with a non-isocyanate route, and the second step may conduct in a triphenylphosphine oxide-catalyzed manner.
研究领域:
有机化学
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药物化学
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DOI
PubMed
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Yixuan Xu,Huiping Dang,Changchang Teng,Dalong Yin,Lifeng Yan
Rapid propagation of tumor cells requires plenty of energy, which is adenosine triphosphate (ATP) dependent. ATP inhibition in tumors not only results in the starvation of tumor cells but also down-regulation of the level of heat shock proteins (HSPs), which usually increase during traditional photothermal therapy (PTT), especially when the temperature is up 50 °C. 2-deoxy-D-glucose (2DG) is an anti-glycolytic reagent and can be used as an efficient agent for ATP inhibition in tumors. Compared with typical PTT, low-temperature mild photothermal therapy (MPTT) is receiving more and more attention because it avoids the high temperatures causing damage to the normal tissue, and the increase of HSPs which decrease PTT. Here, multifunctional polypeptide nanoparticles pDG@Ahx conjugating both a NIR probe Ahx-BDP and 2DG into the side chain of the amphiphilic polypeptide have been prepared. In vitro and in vivo studies reveal that the as-prepared nanoparticles achieve a synergistic effect of starvation/MPTT/PDT (photodynamic therapy), and it provides a new strategy to NIR-I/II fluorescence imaging-guided starvation/MPTT/PDT synergy therapy for tumors.
研究领域:
生物医学工程
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肿瘤学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
Food & Function
·
2022
·Vol. 13
·期 14
·7560-7571
·
IF 6.317
Shuai Lu,Jinyi Zhou,Chen Yang,Xiaoxue Zhang,Yingying Shi,Jie Liu,Xintong Yan,Juanjuan Liang,Xianli Liu,Lan Luo,Da Zhou,Zhimin Yin
Aging is a natural process accompanied by inflammation and oxidative stress and is closely associated with age-related diseases. As a direct precursor of glutathione, γ-glutamylcysteine (γ-GC) possesses antioxidant and anti-inflammatory properties; however, whether γ-GC plays an important role in anti-aging remains unknown. Here, we investigated the protective effects and mechanisms of γ-GC in D-galactose (D-gal)-induced senescence in PC12 cells and aging mice. Our results showed that γ-GC treatment significantly reduced the percentage of senescence-associated-β-galactosidase (SA-β-Gal)-positive cells and inhibited D-gal-induced cell cycle arrest in PC12 cells. The results of Nissl and hematoxylin and eosin (H&E) staining in mouse brain showed that γ-GC treatment markedly reversed the damage in the hippocampus of D-gal-induced aging mice. Moreover, γ-GC increased the phosphorylation of AMP-activated protein kinase (AMPK) to promote the nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) while inhibiting the nuclear translocation of deleted in breast cancer 1 (DBC1), which leads to the activation of sirtuin 1 (SIRT1) and deacetylation of p53 in the nucleus. Therefore, γ-GC may be a potential therapeutic candidate compound for the prevention and treatment of age-related diseases.
研究领域:
药理学
·
衰老
·
神经科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOTECHNOLOGY
·
2018
·
IF 2.533
Binglin Li,Dandan Duan,Jiao Wang,Huanyu Li,Xiaoli Zhang,Binxia Zhao
Phospholipase D (PLD) was firstly hyperactivated by the bio-imprinting and, then, these hyperactivated PLD molecules were immobilized by adsorption and precipitation, followed by cross-linking. The high degree of conformational rigidification provided by intra- and intermolecular cross-linking can make PLD “remember” imprint-induced characteristics even in aqueous solutions. The obtained immobilized PLD showed the excellent catalytic performance. The maximum activity of immobilized PLD reached 166953 U/g protein , which was approximately 14 times higher than that of free form (11922 U/g protein ). Moreover, the selectivity of PLD was significantly enhanced after immobilization. The yield of PG and PA reached 94.0% and 5.96%, respectively. Compared with the serious hydrolysis in the free PLD (35.3% yield of PA), the side reaction was minimized in this work. This may be the first description of the remarkably high improvement of the activity and selectivity of PLD through the immobilization technology.
研究领域:
生物化学
·
酶工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Xue-Bo Liu,Rui Zhao,Min-Min Xu,Shang-Xiong Wei,Xue-Feng Cheng,Jing-Hui He
The electrochemical reduction of nitrate to ammonia (NO3RR) provides a desired alternative of the traditional Haber–Bosch route for ammonia production, igniting a research boom in the development of electrocatalysts with high activity. Among them, molecular electrocatalysts hold considerable promise for the NO3RR, suppressing the competing hydrogen evolution reaction. However, the complicated synthesis procedure, usage of environmentally unfriendly organic solvents, and poor stability of Cu-based molecular electrocatalysts greatly limit their employment in NO3RR, and the development of desired Cu-based molecular catalysts remains challenging. Herein, a simple nonorganic solvent involving a one-step strategy was proposed to synthesize d−π-conjugated molecular electrocatalysts metal–amidinothiourea (M-ATU). Cu-ATU is composed of Cu coordinated with two S and two N atoms, whereas Ni-ATU is formed by Ni with four N atoms from two ATU ligands. Remarkably, Cu-ATU with a Cu–N2S2 coordination configuration exhibits superior NO3RR activity with a NH3 yield rate of 159.8 mg h–1 mgcat–1 (−1.54 V) and Faradaic efficiency of 91.7% (−1.34 V), outperforming previously reported molecular catalysts. Compared to Ni-ATU, Cu-ATU transfers more electrons to the *NO intermediate, effectively breaking the strong sp2 hybridization system and weakening the energy of N═O bonds. The increase in free energy of *NO reduced the energy barriers of the rate-determining step, facilitating the further hydrogenation process over Cu-ATU. Our work opened up a new horizon for exploring molecular electrocatalysts for nitrate activation and paved a way for the in-depth understanding of catalytic behaviors, aligning more closely with industrial demands.
研究领域:
化学
·
环境科学
·
材料科学
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DOI
PubMed
高影响因子
Soft Matter
·
2011
·Vol. 7
·期 14
·6451-6456
·
IF 4.457
Shengtong Sun,Peiyi Wu
Near infrared spectroscopy in combination with two-dimensional correlation spectroscopy (2Dcos) and perturbation correlation moving window (PCMW) technique is employed to illustrate the gelation microdynamic mechanism of hydrogelator N-octyl-D-gluconamide (8-GA), which can rapidly self-agglomerate into helical bilayer micellar fibers upon cooling from spherical micelles. Boltzmann fitting and PCMW easily determined the gelation temperature to be ca. 72 °C and the transition temperature range to be 70–75 °C. Moreover, band shifting and splitting phenomena can be observed for CH-related overtones, indicating the formation of much ordered and tight hydrophobic core from octyl tails. On the other hand, 2Dcos was used to discern the sequential orders during the gelation process and concluded that all the group motions have a continuous transfer from the octyl tail to the chiral carbohydrate head followed by the final immobilization of the solvent, which meanwhile, is actually a continuous dehydration process from the hydrophobic core to the outer hydrophilic chiral head. The driving force of the gelation process in microdynamics can only be the dehydration process of hydrophobic octyl chains, but with final helical superstructures being stabilized by amide-associated hydrogen bonding and the “chiral bilayer effect” of carbohydrate heads.
研究领域:
化学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2023
·
IF 4.5
Haibo Chen,Chengqi Zhao,Yu Li,Junyao Li,Wenrong Cai,Yong Kong,Zheng-Zhi Yin
Au nanoparticles (AuNPs) is first combined with d -methionine ( d -Met) through Au–S bond, and the obtained Au- d -Met was self-assembled with chitosan (CS) via electrostatic driving. Both d -Met and CS are important chiral sources, and therefore the resultant Au- d -Met/CS displays distinct chirality. Interestingly, the chirality of the Au- d -Met/CS can be reversed depending on the ratio of d -Met to CS, as revealed by circular dichroism spectra. Due to the high electrical conductivity of AuNPs as well as the intrinsic chirality of d -Met and CS, the resultant Au- d -Met/CS is used as the electrochemical chiral sensing platform for the discrimination of tryptophan (Trp) enantiomers. Although the Trp enantiomers can be successfully discriminated by the developed chiral sensing platform, the electrochemical signals of l -Trp and d -Trp can be reversed on the differential pulse voltammograms (DPVs) when different ratios of d -Met to CS are used, which can be ascribed to the reversible chirality of the Au- d -Met/CS complexes prepared by different ratios of d -Met to CS.
研究领域:
分析化学
·
生物化学
·
纳米技术
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产品关联
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Beini Chen,Xinrui Tang,Yufei Zhang,Hossain M. Zabed,Yuvaraj Ravikumar,Muhammad Waheed Iqbal,Jiayin Wang,Mei Zhao,Xianghui Qi
d -Allulose, is an improtant rare sugar, having health-promoting properties and applications in the food, pharmaceutical and nutrition sectors. White the aldol reaction-based method shows promise as an alternative to the “Izumori” strategy for ᴅ-allulose bioproduction, its industrial exploitability is hindered by the low product yield in reaction equilibrium and the high costs associated with the use of purified enzymes . To address these limitations, this study explored cell-based biosynthesis of ᴅ-allulose from low-cost glycerol by overexpression the enzymes involved in the ᴅ-allulose aldol condensation (AC) biosynthesis pathway, namely fructose-1-phosphatase (YqaB), alditol oxidase (AldO), and l -rhamnulose-1-phosphate aldolase (RhaD), in Escherichia coli . Results indicated that RhaD is a rate-limiting step of the ᴅ-allulose biosynthetic pathway. Then, biosensor-based high throughput screening was employed for the directed evolution of RhaD. In this way, two RhaD mutants, namely RhaD N156D and RhaD P184S , were successfully screened from 40,129 individual colonies, which exhibited respectively achieved a ᴅ-allulose titer of 3.1 g/L and 2.9 g/L, respectively in shake flasks, representing a more than 20% improvement compared to the wildtype strain. Overall, the ᴅ-allulose biosensor-assisted key enzyme evolution strategy and the high throughput screening method presented in this work provide a paradigm for improving rare sugars’ high-valued productions from cheap substrates.
研究领域:
生物技术
·
代谢工程
·
酶工程
·
合成生物学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）