FOOD CHEMISTRY
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2017
·
IF 4.529
Yao Huang,Fang Feng,Zhi-Gang Chen,Tao Wu,Zi-Han Wang
Natural deep eutectic solvents (NADESs) constitute a novel class of biodegradable and inexpensive solvents. In this study, twenty choline chloride- and glycerol-based NADESs were prepared and investigated as washing agents in the removal of cadmium (Cd) from rice flour for the first time. Choline chloride-based NADESs demonstrated good Cd removal (51%–96%). A natural, biodegradable surfactant, saponin, was mixed with the NADESs to enhance their efficiency. No increase in Cd removal was observed when glycerol-based NADESs were combined with 1% saponin; however, synergistic effects between saponin and choline chloride-based NADESs were observed during the washing process and >99% Cd was removed using NADES-saponin mixtures. Moreover, NADESs washing process did not affect the main chemical components or structure of rice flour. The mechanism of Cd removal by NADESs and regeneration of Cd-contaminated NADESs were also explored. The study presents a green and efficient way of removing Cd from contaminated rice.
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高影响因子
RSC Advances
·
2017
·Vol. 7
·期 48
·29916-29924
·
IF 3.108
Yuanshuai Zhu,Wenzhi Li,Yijuan Lu,Tingwei Zhang,Hasan Jameel,Hou-min Chang,Longlong Ma
A resorcinol-formaldehyde resin carbon (RFC) catalyst with a well-developed, ordered, mesoporous framework was prepared using a soft template method at room temperature. The carbon was sulfonated in water using sulfanilic acid under mild atmospheric conditions. The sulfonated RFC (S-RFC) was characterized by N2 adsorption–desorption, elemental analysis, TEM, XPS, and FT-IR. It was determined that S-RFC is an efficient solid acid catalyst for furfural production from xylose and corn stover in γ-valerolactone (GVL). The effects of reaction time, reaction temperature, catalyst loading, substrate dosage and water concentration were investigated. 80% furfural yield and 100% xylose conversion were obtained from xylose at 170 °C in 15 min with 0.5 g catalyst. Comparatively, 68.6% furfural yield was achieved from corn stover at 200 °C in 100 min when using 0.6 g catalyst. Since there was no discernable decrease in furfural yield after multiple conversions utilizing the same catalyst, the recyclability of the catalyst is considered good.
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FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Jinghong Xu,Jing Zhang,Peihan Wu,Feifei Wang,Zongcai Tu,Hui Wang,Debin Guo
In this work, the effects of Maillard reaction of different monosaccharide-modified fish gelatin were studied. The changes of gel properties, rheology and structure of fish gelatin before and after modification were compared and analyzed, and oil-in-woter emulsions were prepared. The results showed that the five-carbon monosaccharide had stronger modification ability than the six-carbon monosaccharide, which was mainly due to the different steric hindrance of the amino acids in the nuclear layer and the outer layer to the glycosylation reaction. With the progress of the Maillard reaction, the color of fish gelatin gradually became darker. The attachment of sugar chains inhibited the gelation process of fish gelatin, decreased the gelation rate, changed the secondary structure, increased the content of β-turn or α-helix, increased the degree of fluorescence quenching, and enhanced the emulsifying properties and emulsion stability. This study provides useful information for the preparation of different types of monosaccharide-modified proteins and emulsions.
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FOOD HYDROCOLLOIDS
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2024
·
IF 11
Zhen Yang,Chili Zeng,Liang Chen,Tian Tian,Zhongjiang Wang,Hong Wang,Weimin Zhang,Lianzhou Jiang,Zhaoxian Huang,Dongze Li
The study examined the impact of glycation with five-carbon and six-carbon monosaccharides on the structure and physicochemical properties of coconut protein isolate (CPI). Compared to native CPI, CPI/monosaccharide complexes exhibited higher absorbance ratios (A 294 /A 420 ) and glycation degrees. Structural analysis revealed an increase in disordered structures (β-turn and random coil), a blue shift in fluorescence emission, and elevated UV absorbance, indicating protein unfolding and exposure of hydrophobic groups. Glycation significantly enhanced CPI's solubility, emulsifying capacity, oil holding capacity, and thermal stability. Molecular docking simulations emphasized the importance of hydrogen bonding in complex formation. These findings provide insights into protein-carbohydrate interactions, offering potential for diverse industrial applications and guiding future research.
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LWT-FOOD SCIENCE AND TECHNOLOGY
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2024
·
IF 6
Tingting Li,Bingbing Luo,Xiaozhou Zou,Jiamin Yin,Shuyi Lv,Jun Wang,Luchan Gong
γ-Aminobutyric acid (GABA) is a prevalent nonprotein amino acid with diverse physiological functions, attracting considerable interest in the food industry. However, current GABA-producing strains require glutamate as a substrate for synthesis. Probiotics capable of de novo GABA synthesis using sugars without glutamate are crucial for developing novel foods. This study employed a pH indicator method to selectively screen for GABA-producing bacteria. Nineteen strains with fermentation liquid pH greater than 5 were obtained, all demonstrated the ability to synthesize GABA from Daqu . Among these strains, Enterococcus (E.) avium GL1 stood out for its high GABA production capacity, reaching 9.8 g/L when utilizing glucose for de novo GABA synthesis without monosodium glutamate (MSG). E. avium GL1 exhibited robust viability in challenging environments, maintaining stability in simulated gastric and intestinal fluids. As a potentially probiotic strain, E. avium GL1 achieved remarkable GABA production of 206.84 g/L with a production efficiency of 2.87 g/L/h, facilitated by automatic pH control during fermentation through continuous addition of glucose and MSG addition. This exceptional result represents the highest GABA production reported for E. avium , highlighting its significant potential for the development of innovative functional foods.
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Biomass Conversion and Biorefinery
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2024
·
IF 3.5
Wang Xuchong,Wei Yuhe,Sun Xiaoping,Yuan Juan,Chen Haoqiu,Sun Yanfen,Luo Lianxin
Pretreatment is the key step in enzymatic hydrolysis of bamboo wood. Green liquor pretreatment is a promising method to improve enzymatic hydrolysis and saccharification efficiency by changing the physical and chemical properties of lignocellulose surface. At present, there is little work to quantitatively analyze the enzymatic saccharification rate of bamboo lignocellulose by two-stage pretreatment. In this study, a two-stage pretreatment method combining hot water and green liquid phase was mainly used, and the bamboo was treated with Bovine serum albumin (BSA) and Tween80 (TW) chemical additives. After that, the treated samples were hydrolyzed by cellulase, and the effects of pretreatment on the chemical composition and physical properties of bamboo and the influencing factors of chemical additives on the enzymatic hydrolysis efficiency of bamboo were explored. The results showed the two pretreatment stages of hot water and green liquid to have an evident effect on the enzymatic hydrolysis of bamboo. The two-stage pretreatment with hot water and green liquid (14% total alkali) resulted in an 85.51% yield of total sugar hydrolyzed by the enzyme. The addition of BSA and Tween80 improved the efficiency of the enzymatic hydrolysis of bamboo, and the synergistic effect of them could make the total sugar yield of enzymatic hydrolysis reach 99.67%. Through calculation, 65.88 g of fermentable sugar can be obtained per 100 g of bamboo fiber through enzymatic hydrolysis with cellulase. Graphical Abstract
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2024
·
IF 7.7
Wenhui Li,Yuqiu Wu,Si Long,Zhi Chen,Liangzhi Li,Xin Ju
The cascade of sugar isomerases is one of the most practical methods for producing rare sugars, and enzyme immobilization endows it with high economic efficiency, operational convenience and reusability. However, the most employed cross-linker glutaraldehyde (GA) has the disadvantages of enzyme deactivation and limitation of substrate binding. Herein, three compounds, glyoxal, GA, and 2,5-furandicarboxaldehyde (DFF) were evaluated within a previously developed cascade comprising ribose-5-phosphate isomerase and D-tagatose-3-epimerase to prepare D-ribulose form D-xylose. Analyses of surface morphology, element and chemical bond revealed that all compounds effectively cross-linked the isomerases. High concentration of the cross-linkers was generally beneficial for binding protein and preventing enzyme leak during reusing cycles. Glyoxal performed the highest immobilization rate, though it hadn't been employed as a cross-linker for enzyme immobilization. DFF mediated cross-linking revealed the highest activity recovery, substrate conversion and residual activity after reusing cycles, suggesting better biocompatibility than glyoxal and GA. After 8 rounds of recycling, the residual activity of enzyme immobilized by DFF was 61.4 %, ∼30 % higher than that of GA. This study proved a potential alternative cross-linker DFF for the immobilization of enzyme cascade with high activity recovery and reusability, which could promote the efficient production of high value-added products from biomass monosaccharides.
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Frontiers in Nutrition
·
2024
·
IF 4
Wei Shulin,Li Mingxing,Zhao Long,Wang Tiangang,Wu Ke,Yang Jiayue,Tang Mingyun,Zhao Yueshui,Shen Jing,Du Fukuan,Chen Yu,Deng Shuai,Xiao Zhangang,Wei Mei,Li Zhi,Wu Xu
Introduction: Liuweizhiji Gegen-Sangshen beverage (LGS) is popular in China, which has been used for alleviating alcohol-mediated discomfort and preventing alcoholic liver disease (ALD). This beverage is consisted of six herbal components that are known as functional foods and fruits. LGS is rich in polysaccharides, however, the activity and quality evaluation of LGS-derived polysaccharides remain unexplored. The purpose of this study is thus to establish a comprehensive quality control methodology for the assessment of LGS polysaccharides (LGSP) and to further explore the anti-oxidant, anti-inflammatory as well as prebiotic effect of LGSP.Methods: LGSP was extracted, followed by analysis of molecular weight distribution, monosaccharide content and structural characterization via integrating the application of high-performance size exclusion chromatography (HPSEC), 1-phenyl-3-methyl-5-pyrazolone-HPLC (PMP-HPLC), fourier transform infrared spectroscopy (FT-IR) as well as nuclear magnetic resonance spectroscopy (NMR) techniques. The anti-oxidation activity of LGSP was determined by DPPH, ABTS, hydroxyl radical scavenging capacity and total antioxidant capacity. The anti-inflammation of LGSP were assessed on the RAW 264.7 cells. The effect of LGSP on growth of Lactobacillus, Bifidobacterium bifidum and Bifidobacterium adolescentis was evaluated.Results: The results demonstrated that LGSP had two molecular weight distribution peaks, with the average molecular weights of (6.569 ± 0.12) × 104 Da and (4.641 ± 0.30) × 104 Da. LGSP was composed of 8 monosaccharides, with galacturonic acid, glucose rhamnose and galactose representing the highest molar ratios. Homogalacturonic acid (HG) type and rhamnosegalacturonic acid glycans I (RG-I) type and α-1,4-glucan were present in LGSP. LGSP concentration in LGS was 17.94 ± 0.28 mg/mL. Furthermore, fingerprint analysis combined with composition quantification of 10 batches of LGSP demonstrated that there was a high similarity among batches. Notably, LGSP exhibited anti-oxidant effect and inhibited expressions of pro-inflammatory factors (TNF-α and IL-6) in LPS-stimulated RAW 264.7 cells. In addition, LGSP remarkably promoted the proliferation of probiotics Lactobacillus, Bifidobacterium bifidum and Bifidobacterium adolescentis, showing good prebiotic activity.Discussion: The results of present study would be of help to gain the understanding of structure–activity relationship of LGSP, provide a reference for quality evaluation of bioactive LGSP, and facilitate development of unique health and functional products in the future.
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Scientific Reports
·
2024
·Vol. 14
·期 1
·1-9
·
IF 3.8
Sun Yunze,Mi Zhuang,Chen Xiaoyu,Li Jian-Jun,Lu Jun,Shan Xinyan,Lu Xinghua,Du Yuguang
Glycans, nucleic acids and proteins are three major classes of natural biopolymers. The extremely high diversity of isomerization makes structural elucidation of glycans the most challenging job among three classes. In the past few years, the single molecule sensing technique based on nanopores has achieved great success in sequencing of DNA. Inspired by this, it is potential to sequence glycans in the similar manner. Herein, SiN x nanopores were used to identify nine common monosaccharides in mammals. Each monosaccharide showed characteristic blockage current, which roughly increased with the increase of its molecular weight. In order to distinguish nine monosaccharides, several machine learning models were trained and tested, of which the highest F1 value was 1. These results illustrated that nine common monosaccharides in mammals could be clearly identified and discriminate by our method combining solid-state nanopores and machine learning. As far as we know, this is the first report that monosaccharides can be sensed and distinguished by solid-state nanopores. Our work showed the great potential of solid-state nanopores in glycan sequencing, and would lay the foundation for solid-state nanopore-based glycan sequencing.
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Journal of Cleaner Production
·
2024
·
IF 9.7
Li Zhang,Yi Wei,Yuan Yin,Xiang Liu,Shu Zhang,Lei Shi,Juntao Wei,Hong Zhang,Alexander N. Kozlov,Maxim V. Penzik,Deliang Xu
Nitrogen migration and transformation have been extensively studied during the thermochemical conversion of biomass, particularly N-rich biomass, due to concerns about nitrogen oxides pollution or the preparation of N-doped carbon materials. Nitrogen in fiberboard was extracted into an aqueous solution through low-temperature hydrothermal treatment to prepare the recycled aqueous solution. Subsequently, hydrothermal conversions of glucose and xylose were conducted in the recycled solution. The migration and transformation of nitrogen between hydrochars and aqueous phase were investigated. The results showed that the nitrogen retention rates of glucose hydrochars were higher than those of xylose hydrochars at low temperatures. As the temperature increased, the nitrogen retention rates of glucose and xylose hydrochars tended to converge, reaching their highest value at 220 °C. In hydrochars, nitrogen had a high content and was widely distributed on the surface of the carbon microspheres. The nitrogen content did not change significantly with increasing temperatures, but the nitrogen functional groups tended to become more thermostable. In addition, the char yields of glucose and xylose in fiberboard recycled solution were significantly higher than that in the ultrapure water at low temperatures, which indicates that the introduction of nitrogen promotes the formation of hydrochars. These findings favored the study on the nitrogen migration and transformation during hydrothermal conversions of lignocellulose in a N-rich environment.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Kui Zhao,Cheng Qian,Luming Qi,Qing Li,Can Zhao,Jing Zhang,Guiqi Han,Lina Xia,Zeinhom M. El-Bahy,Junwei Gu,Mohamed H. Helal,Zhuyun Yan,Zhanhu Guo,Zhengjun Shi
Acid polysaccharide was extracted from Salvia przewalskii root powders (PSP), purified by diethylaminoethyl cellulose column (DEAE-52) and molecular sieve (PSP2). PSPm1 was obtained by modifying PSP2 with nitrite and phosphoric acid. The chemical structure of PSP2 and PSPm1 exhibited notable distinctions, primarily due to the absence of arabinose and promotion of glucuronic acid (GlcA). The structure of PSPm1 was deduced through the utilization of 1 H, 13 C, and 2-D NMR. The main chain was linked by α-D-Gal p (1 → 3)-α-Glc p -(1 → fragments and →6)-β-D-Gal p fragments, with the presence of →4)-α-D-Glc p A-(1 → 6)-β-D-Gal p -(1 → ， → 4)-α-D-GalA p -(1 → 2,4)-α-D-Rha p -(1 → fragments and →6)-α-Glc p -(1 → 2,4)-β-D-Man p -(1 → fragments. PSPm1 exhibited different immunoregulatory bioactivity in vitro, including haemostatic effects indicated by activated clotting time of 55.5 % reduction by the activated clotting time (ACT) test and wound healing function in vivo. PSPm1 also displayed better anti-tumor biological effects than unmodified. The structure-activity dissimilarity between PSP2 and PSPm1 primarily stems from variations in molecular weight ( Mw ), monosaccharide composition, and branching patterns. The modification of polysaccharides from the extract residues of Chinese medicinal materials may be a new form of drug supplements.
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MOLECULES
·
2018
·Vol. 23
·期 3
·590
·
IF 3.098
Yujing Qin,Qingxia Yuan,Yuexing Zhang,Jialu Li,Xinjiao Zhu,Lingling Zhao,Jing Wen,Jikai Liu,Longyan Zhao,Jinhua Zhao
Enzyme-assisted extraction optimization, characterization and in vitro antioxidant activity of polysaccharides from sea cucumberPhyllophorus proteus(PPP) were investigated in the present study. The optimal extraction conditions with a yield of 6.44 ± 0.06% for PPP were determined as follows: Extraction time of 2.89 h, ratio of extraction solvent to raw material of 16.26 mL/g, extraction pH of 6.83, exraction temperature of 50 °C and papain concentration of 0.15%. Three purified fractions, PPP-1a, PPP-1b and PPP-2 with molecular weights of 369.60, 41.73 and 57.76 kDa, respectively, were obtained from PPP by chromatography of FPA98Cl and Sepharose CL-6B columns. Analysis of monosaccharide compositions showed that PPP-1a consisted ofN-acetyl-galactosamine (GalNAc), galactose (Gal) and fucose (Fuc), PPP-1b of Fuc as the only monosaccharide and PPP-2 of glucuronic acid, GalNAc and Fuc. Sulfate contents of PPP, PPP-1a, PPP-1b and PPP-2 were determined to be 21.9%, 20.6%, 25.2% and 28.0% (w/w), respectively. PPP and PPP-1a had higher molecular weight and intrinsic viscosity than those of the PPP-1b and PPP-2. PPP, PPP-1a, PPP-1b and PPP-2 exhibited obvious activities of scavenging 1,1-diphenyl-2-picrylhydrazyl radical, hydroxyl radical, superoxide radical and ABTS radical in different extent, which suggested that the polysaccharides fromPhyllophorus proteusmay be novel agents having potential value for antioxidation.Keywords:sea cucumber;polysaccharide;enzyme-assisted extraction;characterization;antioxidant activity
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危险品化学品经营许可证（带存储）