MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Wenyun Su,Peng Dai,Xiaotian Xu,Zhaokun Xian,Dong Xing,Chaowen Zheng,Xiaohua Fang,Cuimin Sun,Hui You
Cancer is a significant global health issue, necessitating early detection for effective diagnosis and treatment. We presented a novel method for detecting tumor markers using fluorescence-enhanced photonic crystal hydrogel inverse opal (PC-GELIO) membranes. We efficiently fabricated large-area, low-crack photonic crystal membranes using a homemade microfluidic chip. Composed of PEG-DA and PEG, the hydrogel replicated the photonic crystal structure, yielding photonic crystal hydrogel inverse opal membranes with a unique photonic band gap and inverse opal porous structure. These properties afford the PC-GELIO platform several advantages: Firstly, the high stability of the internal nanostructure in the large-area, low-crack photonic crystals film, prepared using the homemade microfluidic chip, is crucial for preserving the platform's exceptional fluorescence-enhanced sensing capabilities. Secondly, the peak wavelength emitted by fluorescent molecules aligns with the photonic band gap (PBG), significantly enhancing the fluorescence signal through the low-photon effect. Lastly, the high specific surface area provides numerous binding targets for biomolecule modification, and the hydrogel offers a uniform liquid environment conducive to biomolecule binding. We evaluated the fluorescence-enhanced sensing performance of the platform using alpha-fetoprotein (AFP). The limit of quantification (LOQ) for AFP was determined to be as low as 23 pg/mL, representing an order of magnitude improvement compared to non-enhanced platforms (0.29 ng/mL and 0.25 ng/mL). Consequently, this novel fluorescence-enhanced photonic crystal hydrogel inverse opal platform holds significant potential as a straightforward and efficient method for biomolecular detection.
研究领域:
生物医学工程
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分析化学
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材料科学
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癌症研究
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产品关联
DOI
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Pengfei Yan,Junchao Wang,Xiyu Zhao,Liangyu Wang,Jia Xu,Weijia Li,Weihong Min,Dan Wu,Chunlei Liu
QMDDQ is peptide derived from shrimp that ameliorates scopolamine-induced memory impairment. In this study, behavioral experiments in mice showed that QMDDQ could reduce the latency, number of working memory errors of the total distance moved, number of reference memory errors, number of traversals of the original platform, and escape latency in mice. QMDDQ decreased AChE vitality and increased ACh, DA, and 5-HT content. HE staining revealed that the cells in the hippocampal areas exhibited a tight tissue structure, neat arrangement, clear outline, and no cell necrosis in the QMDDQ group. Immunohistochemistry showed that, the expression level of Notch1 protein was reduced, and that of the antibody antigen complex was reduced in the QMDDQ group. Immunofluorescence staining showed a decrease in the number of GFAP and Iba-1 of QMDDQ group. TEM revealed that the mitochondrial cristae are intact and clear of QMDDQ group. QMDDQ decreased expression levels of Notch 1, STAT3, HES1, GFAP, and Iba-1 and activated Notch1 signaling via western-blotting. Pharmacokinetic studies have shown that QMDDQ can be stably absorbed into the bloodstream, with absorption occurring within 12 h and metabolism being completed within 24 h. This study establishes a theoretical basis for active peptides to ameliorate memory impairment and initiates the development of memory-enhancing functional foods.
研究领域:
神经科学
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药理学
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分子生物学
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产品关联
DOI
高影响因子
COMPOSITES PART B-ENGINEERING
·
2024
·
IF 12.7
Zhicun Wang,Cheng Hu,Wen Zhang,Wenqi Liu,Shuyi He,Yang Liu,Li Yang,Yunbing Wang
Following a myocardial infarction (MI) or stroke, the excessive generation of reactive oxygen species (ROS) within a brief timeframe may induce a pronounced inflammatory reaction, which exacerbates the progression of the disease. Thus, there is an urgent need to design a biomaterial to inhibit the inflammatory response associated with MI and stroke. In this study, we designed a reversible extracellular matrix (ECM)-like hydrogel via borate ester crosslinking, which responds to the inflammatory microenvironment affected by MI and stroke. At the same time, we developed a new type of loaded polymer nanoparticle (TK-DA) that can respond to ROS in ischemic regions and release the anti-inflammatory drug dexamethasone. Ultimately, we integrated the nanoparticles and ECM-like hydrogel to fabricate a functional hydrogel. To verify the therapeutic effect of the functional hydrogel, we established two models of typical ischemic diseases, MI and stroke. The results showed that the hydrogel substantially reduced ROS levels and inflammation in vitro. Moreover, the results of the two in vivo animal models demonstrated that this functional hydrogel can effectively treat MI and stroke by suppressing inflammatory responses, mitigating apoptosis, and promoting neovascularization. In conclusion, the functional hydrogel strategy presents a universally applicable treatment approach for ischemic cardiovascular and cerebrovascular diseases, which are not limited solely to the treatment of MI and stroke, but also hold future potential for clinical applications.
研究领域:
生物医学工程
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药学
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心血管疾病
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神经科学
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产品关联
DOI
高影响因子
ELECTROCHIMICA ACTA
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2020
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IF 6.215
Mingmei Shen,Xianwen Kan
Specifically sensitive determination of dopamine (DA) from complex real samples is very important since DA plays a major role in the function of central nervous, which presents the necessary to develop an effective sensor for the assay of DA. In this work, a dual recognition strategy was developed through introducing DA-aptamer and molecularly imprinted polymer (MIP) on gold nanoparticles (AuNPs) and reduced graphene oxide (rGO) modified electrode. The selectivity and sensitivity of the prepared sensor were investigated upon the current response of DA itself to better represent the performances of the sensor. Compared with the single recognition element (MIP or aptamer) based sensors, the prepared dual recognition sensor displayed the highest selectivity to DA, which facilitated the proposed sensor for the detection of DA in real samples. Owing to the high surface and good conductivity of the modified AuNPs and rGO, the sensor showed a wide linear range and a low limit of detection of 5.0 × 10 -8 – 1.0 × 10 -5 mol/L and 4.7 × 10 -8 mol/L for the detection of DA, respectively. The proposed strategy of aptamer and MIP based sensor for electro-active target assay may be used as a general way for the development of other dual recognition based sensors.
研究领域:
分析化学
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生物传感器
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纳米技术
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神经化学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry B
·
2021
·Vol. 10
·期 14
·2602-2616
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IF 6.331
Yushui He,Xiao Wang,Yuanyuan He,Xin Zhao,Jingjing Lin,Yuan Feng,Jie Chen,Feng Luo,Zhen Li,Jiehua Li,Hong Tan
Guided tissue regeneration (GTR) is the main therapeutic method for periodontal tissue regeneration. The key to the GTR strategy is the membrane which can assist the reconstruction of bone tissue in the periodontal defect and prevent the migration of epithelium and fibroblasts to the defect. However, the existing periodontal membrane cannot effectively promote periodontal tissue regeneration due to the limited bioactivity and physicochemical function. Here, we developed a bioinspired degradable polyurethane membrane with Janus surface morphology by integrating bioactive dopamine (DA) and an antibacterial Gemini quaternary ammonium salt (QAS). The Janus surface of the membrane is fabricated through spontaneous microphase separation, resulting from the different migration of functional segments between the air-contact upper surface with enriched antibacterial QAS and the substrate-contact bottom with enriched bioactive DA. The smooth surface of the upper membrane used to face the soft tissues can reduce cell adhesion to suppress the migration of fibroblasts, while the rough surface with a topological micro-pit structure of the bottom side facing the bone has excellent function of autonomic mineralization and cell adhesion to promote bone tissue reconstruction. In addition, the membrane containing the antibacterial QAS shows excellent antibacterial effect on common oral pathogens, such as S. aureus and S. mutans. Moreover, the specific dopamine group also endows the membrane with excellent antioxidant efficiency. In vivo research shows that this Janus polyurethane membrane can effectively promote periodontal tissue regeneration in a rat periodontal defect model. Combined with its excellent mechanical properties and biocompatibility, the polyurethane membrane is a promising material for potential periodontal tissue regeneration.
研究领域:
生物材料
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组织工程
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牙科材料
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再生医学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2026
·
IF 10.4
Hui-Wen Wang,Jia-Qi Wang,Yue-Qiu,Tong-Hao Cui,Jian-Cong Liao,Yi-Long Liu,Ya-Qin Liu,Yuan-Jiang Pan,Xian Li,Xiao-Yong Zhao
Universal 5-Da labeling creates internal standards for accurate N-glycome analysis. • Novel matrix 2-AIA/CHCA ensures uniform co-crystallization and high reproducibility. • H3N2F1X1 is a cross-species candidate biomarker for fruit cold stress. N-glycosylation is a crucial post-translational modification regulating protein function in plants. However, its accurate quantification by matrix-assisted laser desorption/ionization-mass spectrometry with time-of-flight is challenged by low glycoprotein abundance, poor glycan ionization, uneven matrix crystallization, and insufficient internal standards. Here, we introduce a novel 5-Da stable isotope labeling method, combining enzymatic 18 O-labeling (+2-Da) and reduction with NaBD 4 (+3-Da) on a portion of the sample itself to universally generate internal standards that eliminate isobaric interference. Furthermore, a binary matrix of 2-aminoisophthalic acid (2-AIA) plus α-cyano-4-hydroxycinnamic acid (CHCA) was developed, providing high sensitivity and homogeneous co-crystals. The integration of isotope labeling with 2-AIA/CHCA allows linear quantification ( R 2 > 0.99) over a two orders of magnitude. Applied to tomato and peach during cold storage, the method revealed that H3N2F1X1, the most abundant N-glycan in both fruits, was significantly downregulated, suggesting its role as a candidate biomarker for cold stress response and fruit quality control. Download: Download high-res image (204KB) Download: Download full-size image
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2020
·
IF 3.209
Lijuan Huang,Tongtong Li,Yingjun Zhang,Xiaohong Sun,Yongan Wang,Zhiyong Nie
The detection of psychoactive substances is an important branch of modern analytical chemistry and has many legally and socially relevant implications. The use of a surface plasmon resonance (SPR)-based gene–nanoparticle system has emerged as a promising technique for the rapid and ultrasensitive detection of molecular species such as drugs of abuse in biofluids. However, the development of a viable screening tool for the detection of multiple classes of drugs in complex media is a considerable challenge because the existing techniques lack affinity toward certain species due to matrix interference. Our aim was to develop a simple optical sensor array for the classification of nine narcotic drugs in aqueous solution and human urine. The UV–vis spectra of DNA–gold nanoparticles in the presence of nine narcotic drugs (pentobarbital sodium, caffeine, morphine, remifentanil, fentanyl, ketamine, etomidate, carfentanil, and sulfentanyl) were distinctly different. Furthermore, the narcotic drugs present in aqueous solution and in human urine were classified correctly through partial least squares discriminant analysis (PLS-DA). Combination with a multi-sensor unit further improved the prediction accuracy of the PLS-DA models. The proposed method has potential for on-site drug detection and drug abuse screening.
研究领域:
分析化学
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纳米技术
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化学计量学
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法医学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Dechan Lu,Yanping Chen,Longfeng Ke,Weilin Wu,Liwen Yuan,Shangyuan Feng,Zufang Huang,Yudong Lu,Jing Wang
DNA methylation plays a critical role in the development of human tumors. However, routine characterization of DNA methylation can be time-consuming and labor-intensive. We herein describe a sensitive, simple surface-enhanced Raman spectroscopy (SERS) approach for identifying the DNA methylation pattern in early-stage lung cancer (LC) patients. By comparing SERS spectra of methylated DNA bases or sequences with their counterparts, we identified a reliable spectral marker of cytosine methylation. To move toward clinical applications, we applied our SERS strategy to detect the methylation patterns of genomic DNA (gDNA) extracted from cell line models as well as formalin-fixed paraffin-embedded tissues of early-stage LC and benign lung diseases (BLD) patients. In a clinical cohort of 106 individuals, our results showed distinct methylation patterns in gDNA between early-stage LC (n = 65) and BLD patients (n = 41), suggesting cancer-induced DNA methylation alterations. Combined with partial least square discriminant analysis, early-stage LC and BLD patients were differentiated with an area under the curve (AUC) value of 0.85. We believe that the SERS profiling of DNA methylation alterations, together with machine learning could potentially offer a promising new route toward the early detection of LC.
研究领域:
癌症生物学
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分子生物学
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生物医学工程
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生物信息学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Dongsheng Song,Congcong An,Zhe Zhang,Yu Qiu,Zhimin Zhao,Dingqiang Zheng,Ming Zhang
A superhydrophobic CNF/PDA composite membrane has been developed to revolutionize oily wastewater treatment for achieving high efficiency, cost-effectiveness, and environmental sustainability. Inspired by the adhesive properties of mussels and the self-cleaning mechanism of lotus leaves, this innovative material is fabricated through a novel lyophilization and spray modification process using cellulose nanofibers (CNF), dopamine hydrochloride (DA), and octadecylamine (ODA). The membrane is synthesized via an aerobic, weakly alkaline self-polymerization process, which facilitates the formation of robust covalent and non-covalent bonds between the catecholamine structure of DA and CNF. ODA is integrated through hydrogen bonding and Schiff base reactions with polydopamine (PDA), enhancing structural stability. The resulting membrane demonstrates a tensile strength of 2.9 MPa, alongside exceptional separation efficiency (98.5 %) and flux rates (3745 L·m −2 ·h −1 ). Even after five consecutive water-in-oil (W/O) emulsion separation cycles, a separation efficiency exceeding 91.8 % is maintained, with hydrophobicity and structural integrity preserved. Durability tests confirm its robustness, with 98.5 % of initial capacity retained after ten adsorption-desorption cycles. The membrane's porous architecture and photothermal brown-black hue synergistically boost light-to-heat conversion, facilitating swift thermal response and efficient high-viscosity oil uptake. This demonstrates viable applications as a sustainable solution for oily wastewater treatment, promoting greener industrial application.
研究领域:
环境科学
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材料工程
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化学工程
·
绿色技术
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Yong Zhu,Likang Qin,Yue Chen,Shaoqi Zhou
The release patterns of phenolics in digestive tract was elucidated using a simulated digestion protocol, in which the delivery route of rutin was revealed, and the availability of released phenolics was also evaluated using a dialysis membrane absorption model. The results showed that phenolic compounds such as rutin, quercetin , 3,4-dihydroxybenzoic acid (DA), 2,3,4-trihydroxybenzoic acid (TA) and chlorogenic acid (CA) were released from Tartary buckwheat under simulated digestion. The contents of released phenolic compounds in oral-gastrointestinal digestion were significantly higher ( p < 0.05) than that in oral and oral-stomach digestion phases. Besides, in oral-gastrointestinal digestion, the proportion of available contents of rutin, DA, TA and CA to corresponding released contents were 71.8%, 73.7%, 68.6% and 100%, and the great majority of rutin was sufficiently degraded into quercetin. The contribution of antioxidant activity of available compounds to corresponding total activity were between 62.2% and 68.9%. These results indicated that phenolics in Tartary buckwheat can be adequately released in oral-gastrointestinal digestion, and a great majority of these phenolics are available. This study illustrates the potential bioavailability of phenolic compounds in Tartary buckwheat during gastrointestinal digestion.
研究领域:
食品科学
·
营养学
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药理学
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产品关联
DOI
高影响因子
Nanoscale
·
2022
·Vol. 14
·期 38
·14122-14128
·
IF 8.307
Chenchao Huang,Zishou Hu,Yuan-Qiu-Qiang Yi,Xiaolian Chen,Xinzhou Wu,Wenming Su,Zheng Cui
Manufacturing cost is a major concern for electrochromic device (ECD) applications in smart windows for energy saving and low-carbon economy. Fully printing instead of a vacuum-based chemical vapor deposition (CVD) process is favored for large-scale fabrication of ECDs. To adapt to the screen printing process, a UV curable solid-state electrolyte based on lithium bis(trifluoromethane-sulfonyl) imide (LiTFSI) was specially formulated. It contains poly(ethylene glycol) diacrylate (PEG-DA), LiTFSI, water, and ethyl acetate. The optimized ECDs have achieved a 0.6 s bleaching time at 0.6 V and a 1.4 s coloring time at −0.5 V. The ECDs also exhibited excellent stability, which could endure 100 000 cycles of color switching while still maintaining 35% of transmittance change at a 550 nm wavelength. A demo ECD has been fabricated with a screen printed electrolyte, exhibiting stable switching between the clear state and patterned color state.
研究领域:
材料科学
·
电化学
·
能源效率
·
制造技术
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Jia-Nuo Zhang,Zhi-Dong Pei,Wen-Yu Wang,Ming-Yue Zhao,Wen-Han Pei,Hui Zhang,Hai-Bo Yin,Tian-Min Wang,Gui-Zhong Xin,Ming Xie,Ting-Guo Kang,Yue-Hua Chen,Hui-Peng Song
Zanthoxyli radix is a popular tea among the elderly, and it is believed to have a positive effect on Alzheimer’s disease. In this study, a highly effective three-step strategy was proposed for comprehensive analysis of the active components and biological functions of Zanthoxylum nitidum (ZN), including high-resolution LC-Q-TOF mass spectrometry (HRMS), multivariate statistical analysis for heterogeneity (MSAH), and experimental and virtual screening for bioactivity analysis (EVBA). A total of 117 compounds were identified from the root, stem, and leaf of ZN through HRMS. Bioactivity assays showed that the order of acetylcholinesterase (AChE) inhibitory activity from strong to weak was root > stem > leaf. Nitidine, chelerythrine, and sanguinarine were found to be the main differential components of root, stem, and leaf by OPLS-DA. The IC50 values of the three compounds are 0.81 ± 0.02, 0.14 ± 0.01, and 0.48 ± 0.01 μM respectively, indicating that they are potent and high-quality AChE inhibitors. Molecular docking showed that pi-pi T-shaped interactions and pi-lone pairs played important roles in AChE inhibition. This study not only explains the biological function of Zanthoxyli radix in alleviating Alzheimer’s disease to some extent, but also lays the foundation for the development of stem and leaf of ZN.
研究领域:
药理学
·
分析化学
·
神经科学
·
植物科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）