BIORESOURCE TECHNOLOGY
·
2012
·
IF 4.98
Zhixing He,Hailong Xiao,Lu Tang,Hang Min,Zhenmei Lu
HD-1, a stable microbial consortium capable of mineralizing di- n -butyl phthalate (DBP), was developed from activated sludge. The dominant microorganisms in the consortium, Gordonia sp., Burkholderia sp. and Achromobacter sp., were identified by denaturing gradient gel electrophoresis (DGGE). The consortium could mineralize approximately 90% of 1200 mg/L DBP after 48 h of cultivation. The optimal DBP degradation conditions were 25–30 °C and pH 8.0–9.0. The addition of yeast (0.5 g/L), sodium acetate (0.5 g/L, 1.0 g/L), Brij 35 (0.2%, 0.5%, 1.0%), or Triton X-100 (0.2%) enhanced DBP degradation. The DBP degradation rate was influenced by the presence of dimethyl phthalate (DMP) and diethyl phthalate (DEP). Only one main intermediate, phthalic acid, could be monitored by gas chromatography–mass spectrometry (GC–MS) during the degradation process. The HD-1 consortium also utilized phenol, o -dihydroxybenzene as the sole carbon and energy source. The results indicate the consortium may represent a promising application for DBP bioremediation.
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 31
·31091-31100
·
IF 2.8
Lei Yu,Zhu Chengzhu,Lu Jun,Zhu Yongchao,Zhang Qiuyue,Chen Tianhu,Xiong Hongbin
The photochemical oxidation of di- n -butyl phthalate (DBP) by • OH radicals from nitrous acid (HONO) in atmospheric hydrometeors was explored by two techniques, steady-state irradiation, and laser flash photolysis (LFP). The effects of atmospheric liquid parameters on DBP transformation were systematically evaluated, showing that DBP does not react with HONO directly and • OH-initiated reactions are crucial steps for consumption and transformation of DBP. Two reaction channels are operative: • OH addition and hydrogen atom abstraction. The overall rate constant for the reaction of DBP with • OH is 5.7 × 10 9 M −1 s −1 , and its specific rate constant for addition is 3.7 × 10 9 M −1 s −1 determined by using laser flash photolysis technique. Comparing the individual reaction rate constant for aromatic ring addition with the total rate constant, the majority of the • OH radicals (about 65%) attack the aromatic ring. The major transformation products were identified by GC-MS, and the trends of their yields derived from both ring addition and H-abstraction with time are discussed. These results provide important insights into the photochemical transformation of DBP in atmospheric hydrometeors and contribute to atmospheric aerosol chemistry.
研究领域:
环境科学
·
大气化学
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DOI
PubMed
高影响因子
Food Analytical Methods
·
2021
·Vol. 14
·期 11
·2375-2385
·
IF 3.366
Zhao Yuanli,Zhu Zhenbao,Liu Jianghua,Liu Jichao,Li Guoliang
Phthalate esters (PAEs) are prone to migrate from the plastic containers into oil during production and storage process. Owing to the trace amount of PAEs and complex sample matrix, it is of great importance to explore a facile and sensitive way to extract and detect PAEs from oil samples. In this work, magnetic covalent organic framework nanospheres (Fe 3 O 4 @COF(TbBD)) were fabricated by the aldehyde-amine condensation between benzidine (BD) and 1,3,5-triformylbenzene (Tb) on the surface of Fe 3 O 4 nanoparticles with the help of ultrasonic treatment. The new type nanomaterials were firstly used to the magnetic solid-phase extraction (MSPE) of seven PAEs in edible vegetable oils prior to high performance liquid chromatography coupled with a diode array detector (HPLC–DAD). In order to achieve the best extraction efficiency, several key parameters were optimized. Under optimal conditions, the proposed Fe 3 O 4 @COF(TbBD)-MSPE-HPLC–DAD method possessed high sensitivity (0.55–0.95 µg/kg for LODs and 1.80–3.10 µg/kg for LOQs), good linearity ( R 2 ≥ 0.9983), and satisfactory recoveries (80.2–102.9%). The RSDs for intra-day was less than 6.1%, and inter-day was less than 7.9%. This work not only proposed a rapid and reliable approach for determining PAEs in edible vegetable oils but also broadened the application of magnetic COFs in food determination.
研究领域:
食品科学
·
分析化学
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DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2015
·
IF 4.143
Hai-Ming Zhao,Huan Du,Lei Xiang,Yi-Liang Chen,Lei-An Lu,Yan-Wen Li,Hui Li,Quan-Ying Cai,Ce-Hui Mo
Phthalate ester (PAE) accumulation in crops poses great risks to human health and has aroused great concern. Here, we investigated variations in di- n -butylphthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) accumulation by various Chinese flowering cabbage cultivars and revealed their variation mechanism. There were significant differences ( p < 0.05) in shoot PAE concentrations of 28 cultivars. Moreover, significant positive correlations between DBP and DEHP concentrations in shoots of all cultivars indicated that they could be taken up simultaneously by various cultivars. Due to the lower translocation factor of low-PAE accumulator, its shoot PAEs concentrations were much lower than root compared to high-PAE accumulator. Further, subcellular distribution showed that PAE concentrations of root cell walls and organelles were much higher than those of shoots in low-PAE accumulator. Therefore, lower translocation from root to shoot and more PAEs accumulating in cell walls and organelles of root might act as main formation mechanism of low-PAE accumulator.
研究领域:
环境科学
·
植物学
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DOI
PubMed
高影响因子
BIOLOGY AND FERTILITY OF SOILS
·
2015
·Vol. 52
·期 1
·65-76
·
IF 3.398
Zhao Hai-Ming,Du Huan,Feng Nai-Xian,Xiang Lei,Li Yan-Wen,Li Hui,Cai Quan-Ying,Mo Ce-Hui
A novel 2D strain that could effectively degrade di- n -butylphthalate (DBP) and its major metabolite, phthalic acid (PA), was isolated from compost and identified as Providencia sp . 2D. A complete degradation of DBP (200 mg L −1 ) was observed within 3 days under optimal conditions obtained by response surface methodology. This strain 2D could utilize various phthalic acid esters (PAEs) as substrates for growth, and could co-metabolize DBP in the presence of extra C sources. A novel combination of two common pathways in PA degradation was proposed for DBP degradation pathway, representing the first report of two ring cleavage pathways of the intermediate PA in a microbial species. Strain 2D efficiently enhanced the removal rate of DBP in contaminated soil with a sharp decrease of the DBP half-life compared to non-bioaugmentation treatments. Moreover, the addition of compost improved the degradation rate of DBP in soil by stimulating microbial activity. The results support the feasibility of remediating DBP-contaminated soils inoculated with strain 2D and treated with compost.
研究领域:
环境微生物学
·
土壤科学
·
生物修复
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DOI
高影响因子
ADVANCES IN POLYMER TECHNOLOGY
·
2015
·Vol. 36
·期 2
·220-229
·
IF 1.045
Xiaoming Zhang,Wenming Yang,Ningwei Wang,Xiaoni Ni,Huajie Wang,Zhaoyang Gu,Xiangyang Wu,Wanzhen Xu
New ionic liquid-mediated molecularly imprinted polymers (ILMIPs) for phthalate esters were developed in this study by the situ thermal polymerization method using methacrylic acid as a functional monomer and dibutyl phthalate (DBP) as a template. The polymer was characterized by scanning electron microscopy and Fourier transform infrared spectrometry. Batch static binding adsorption experiments were carried out to analyze its adsorption performance. The selectivity and adsorption of molecularly imprinted polymers were improved by the ionic liquids 1-butyl-3-methylimidazolium hexafluorophosphate as an excellent auxiliary porogen and solvent. The adsorption process conformed to pseudo–first-order model by kinetics analysis and Sips model by isotherm analysis. Using the prepared ILMIPs as a solid-phase extraction (SPE) sorbent, which indicated that ILMIPs–SPE had a higher enrichment efficiency and a better selectivity for DBP, and the average recoveries of DBP at four spiked levels were in a range of 96.4–101.3% ( n = 5) with relative standard deviation (RSD) ≤6.9%.
研究领域:
环境科学
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分析化学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2014
·Vol. 37
·期 24
·3656-3661
·
IF 2.594
Xi Luo,Chengxia He,Feifang Zhang,Hailong Wang,Bingcheng Yang,Xinmiao Liang
Heat-shrink tubing, which shrinks in one plane only (its diameter) when heated, commonly used for sealing protection in electrical engineering, was found to be able to function as a solid-phase microextraction coating. Its utility was demonstrated for the determination of phthalic acid esters in an aqueous solution combined with high-performance liquid chromatography equipped with a UV absorbance detector. The preparation procedure was rather simple and only ∼10 min was needed. The fiber cost is extremely low (∼10 cent each). The parameters affecting the extraction were optimized. Heat-shrink tubing fiber exhibited a significant enrichment effect for the three examined phthalic acid esters and up to 931-fold enrichment factor was obtained. The limit of detection was <10 μg/L for all analytes. The operation repeatability and fiber-to-fiber reproducibility were 1.2–8.3 and 5.4–9.1%, respectively. It was successfully applied for the analysis of bottled drinking water with recoveries ranging from 90.1–100.5%.
研究领域:
分析化学
·
环境科学
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DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2014
·Vol. 38
·期 3
·445-452
·
IF 2.594
Man Wang,Xiaodi Yang,Wentao Bi
Magnetic graphitic carbon nitride nanocomposites were successfully prepared in situ and used to develop a highly sensitive magnetic solid-phase extraction method for the preconcentration of phthalate esters such as di- n -butyl phthalate, butyl phthalate, dihexyl phthalate, and di-(2-ethyl hexyl) phthalate from water. The adsorption and desorption of the phthalate esters on magnetic graphitic carbon nitride nanocomposites were investigated and the parameters affecting the partition of the phthalate esters, such as adsorption, desorption, recovery, were assessed. Under the optimized conditions, the proposed method showed excellent sensitivity with limits of detection (S/N = 3) in the range of 0.05–0.1 μg/L and precision in the range of 1.1–2.6% ( n = 5). This method was successfully applied to the analysis of real water samples, and good spiked recoveries over the range of 79.4–99.4% were obtained. This research provides a possibility to apply this nanocomposite for adsorption, preconcentration, or even removal of various carbon-based ring or hydrophobic pollutants.
研究领域:
分析化学
·
环境科学
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DOI
PubMed
高影响因子
PLoS One
·
2017
·Vol. 12
·期 5
·e0178213
·
IF 2.806
Yangyang Wang,Fangfang Li,Xinling Ruan,Jian Song,Lv Lv,Liyuan Chai,Zhihui Yang,Lin Luo
A stable bacterial consortium (LV-1) capable of degrading di- n -butyl phthalate (DBP) was enriched from river sludge. Community analysis revealed that the main families of LV-1 are Brucellaceae (62.78%) and Sinobacteraceae (14.83%), and the main genera of LV-1 are Brucella spp. (62.78%) and Sinobacter spp. (14.83%). The optimal pH and temperature for LV-1 to degrade DBP were pH 6.0 and 30°C, respectively. Inoculum size influenced the degradation ratio when the incubation time was < 24 h. The initial concentration of DBP also influenced the degradation rates of DBP by LV-1, and the degradation rates ranged from 69.0–775.0 mg/l/d in the first 24 h. Degradation of DBP was best fitted by first-order kinetics when the initial concentration was < 300 mg/l. In addition, Cd 2+ , Cr 6+ , and Zn 2+ inhibited DBP degradation by LV-1 at all considered concentrations, but low concentrations of Pb 2+ , Cu 2+ , and Mn 2+ enhanced DBP degradation. The main intermediates (mono-ethyl phthalate [MEP], mono-butyl phthalate [MBP], and phthalic acid [PA]) were identified in the DBP degradation process, thus a new biochemical pathway of DBP degradation is proposed. Furthermore, LV-1 also degraded other phthalates with shorter ester chains (DMP, DEP, and PA).
研究领域:
环境科学
·
微生物学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2017
·
IF 2.603
Zigang Tang,Lanling Chu,Yu Wang,Yuan Song,Ping Liu,Jinhui Fan,Jinjin Huang,Xiongwei Liu,Lanlan Wei,Chen Li,Renshan Zhao,Xuejun Kang
In this paper, we developed a rapid and safe method based on packed-fiber solid-phase extraction (PFSPE) system coupled with gas chromatography-mass spectrometry for the determination of four phthalate esters (PAEs), diethyl-o-phthalate (DEP), dibutyl-o-phthalate (DBP), di (2-ethylhexyl) phathalate (DEHP), di- n -octyl phthalate (DNOP), in urine samples. The PAEs in urine samples (500 μL) were rapidly cleaned up from urines using polystyrene (PS) nanofibers packed micro-columns fitted on a PFSPE pretreatment device, which can process up to 12 samples simultaneously in 5 min. Under optimum conditions, satisfied recovery and relative standard deviation values (RSDs) were in the range of 80.4–111.7% and 1.5–10.9%, respectively. The limits of detection (LOD) and the limits of quantification (LOQ) were ranged from 0.1 to 0.5 ng mL −1 and 0.5–2 ng mL −1 , respectively. The well controlled matrix effect was also evaluated by comparing the signal response of the pure PAEs standards dissolved in methanol with the signal response of PAEs in the urine matrix. This new method was successfully applied to determine four PAEs in urine samples of overweight and normal-weight children, and an association between phthalates in urines and obesity was observed. Thus, the method seems to be a useful tool for monitoring of the level of urinary phthalate esters and also to support an evidence for further reasearch of obesity.
研究领域:
环境科学
·
分析化学
·
公共卫生
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DOI
PubMed
高影响因子
PEDOSPHERE
·
2017
·
IF 1.734
Jiajun DENG,Yanling ZHANG,Jiwei HU,Jiaguo JIAO,Feng HU,Huixin LI,Shixiang ZHANG
Autotoxicity is one of the major factors that impede continuous cropping. It is defined as the toxic influence of chemicals released from one plant species on the germination and growth of individuals of the same species. Here, in order to exam the autotoxicity of tobacco root exudates, root exudates were collected from tobacco plants grown both in cultural solution and on natural soil. Using ultra-performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry, main autotoxic chemical substances in the root exudates were identified. The autotoxic effects of suspected autotoxins on seed germination (including germination rate, germination potential, germination index, and vigor index) and seedling growth were analyzed. Dibutyl phthalate (or diisobutyl phthalate), dioctyl phthalate, and diisooctyl phthalate were identified in tobacco root exudates. It was observed that high concentrations (greater than 0.5 mmol L −1 ) of each identified phthalate ester caused significant ( P < 0.05) inhibition of tobacco seed germination and seedling growth. It can be concluded that phthalate esters such as dibutyl phthalate, diisobutyl phthalate, and diisooctyl phthalate in tobacco root exudates may play an important role in tobacco autotoxicity.
研究领域:
植物学
·
生态学
·
农业科学
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DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2017
·
IF 6.216
Ghaffar Abdul,Xiaoying Zhu,Baoliang Chen
To create more active sites on biochar surface, biochar-graphene (BG) nanosheet composites were prepared by one step facile dip coating following thermal route at three different temperatures, in addition to biochars (300, 500, 700 °C). The morphology and structural composition of biochars and BG composites were examined by SEM, TEM, BET–N 2 and CO 2 , Raman, FTIR, XPS, TGA and CHN elemental analysis. It was found that graphene nanosheets (GNS, ∼1 µm, 0.1% mass) could ensure relatively higher surface area (N 2 and CO 2 ), porous structure and thermal stability within BG composites. BG composites portrayed the existence of GNS bearing cavities and evidently increased the graphitic structure. The adsorption capabilities of biochars and BG composites towards dimethyl phthalate (DMP), diethyl phthalate (DEP), and dibutyl phthalate (DBP) as model phthalic acid esters (PAEs) were examined by batch sorption technique. The BG composites exhibited the increased adsorption capacity comparatively to biochars. The pseudo-second-order kinetic rate ( k 2 ) illustrated that adsorption of PAEs was efficient and smaller molecule (DMP) was quicker to pore-diffusion mechanism. The aromatic sheets of biochars and GNS on biochars dominated the π–π EDA (electron donor–acceptor) interaction for ring structure of DMP molecule, whereas adsorption of DBP was attributed to hydrophobicity. The surface morphology and composition of biochars can be regulated with GNS to promote the adsorption capacity and kinetics for effective pollutant remediation and could be considered as promising adsorbent for various organic contaminants.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）