SEPARATION AND PURIFICATION TECHNOLOGY
·
2021
·
IF 7.312
Qin Li,Binjin Zhao,Jing Shi,Jianbo Chu,Xinyue Zhang,Qiyuan Shi
In this study, the potential of supramolecular solvent/equilibrium solution (SUPRAS/EqS) for the extraction of caffeoylquinic acids from the flowers of Artemisia anomala (AAF) coupled with re-extraction was evaluated. Extraction and re-extraction parameters were optimized based on the yield of 3-chlorogenic acid, isochlorogenic acid A-C. The DPPH radical scavenging and xanthine oxidase inhibitory activity of the final extracts were also evaluated. SUPRAS/EqS, made up by n -octanoic acid, ethanol, 5% acetic acid aqueous solution, offered advantages in terms of rapidity (extraction for 1 min) and simplicity (the process involved vortex-shaken and centrifugation at room temperature), thus avoiding cost processes based on high pressure or temperature. Multi-stage countercurrent extraction (MSCE) procedure was successfully applied to reduce the consumption of extraction solvent (24-fold volume of raw materials in total). SUPRAS/EqS-MSCE extracts were evaporated to recycle ethanol and then re-extracted by water for 3 times (with a total consumption of 9-fold volume of material weight). The contents of 3-chlorogenic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C in the final extract were 3.61 ± 0.01, 2.32 ± 0.01, 26.42 ± 0.05, 5.38 ± 0.01 mg/g dry weight of AAF with a recovery rate of 95.0%, 92.7%, 90.6% and 94.5%, respectively. Furthermore, the final extract of AAF exhibited moderate DPPH radical scavenging activity (IC 50 value was 839. 46 ± 1.02 μg/mL, vitamin C’s was 220.29 ± 1.02 μg/mL) and weak xanthine oxidase inhibitory activity (IC 50 value was 4518.56 ± 1.03 μg/mL, quercetin’s was 103.04 ± 1.04 μg/mL).
研究领域:
植物化学
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提取技术
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2017
·
IF 3.671
Yichen Hu,Jinming Zhang,Liang Zou,Chaomei Fu,Peng Li,Gang Zhao
Chenopodium quinoa , a promising nutraceutical cereal, has attracted increasing research interest, yet its polysaccharides remains to get few systematic studies. In this study, we employed orthogonal experimental design to optimize the ultrasound-assisted extraction process for highest yield of C. quinoa polysaccharides. A novel C. quinoa polysaccharide (CQP) fraction with high content and low molecular weight (8852 Da) was subsequently purified by column chromatography, constituted by galacturonic acid and glucose monosaccharides. The purified CQP exhibited significantly antioxidant effect against DPPH + and ABTS + , with even higher efficiency than some other reported polysaccharides. Moreover, CQP could promote the RAW264.7 macrophage proliferation, while suppress the nitri oxide production on inflammatory RAW264.7 macrophage in a dose- and time-dependent manner. In view of the pathological correlation of free radical, inflammation and carcinogenesis, the anticancer effect of CQP was further investigated on human liver cancer SMMC 7721 and breast cancer MCF-7 cells. Interestingly, CQP displayed cytotoxicity against cancer cells, while none proliferation inhibition on normal cells. These results suggest that the bioactive polysaccharide from C. quinoa provided the promising potential as a natural antioxidant, immune-regulating and anticancer candidate for food and even drug application.
研究领域:
营养保健品
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药理学
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生物化学
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免疫学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2025
·
IF 2.5
Ke Guo,Peng Zhao,Xinxin Yang,Jin Wang,Wei Chang,Simei Liu,Siqi Zhang,Tingting Zhang,Hao Yan,Yafeng Yan,Jie Wang,Xiping Chen,Churong Yu,Guoquan Wang
The present study reports the synthesis of a selenium nanocomplex (Se-TFPs) using purified polysaccharides from Tussilago farfara L (coltsfoot). It evaluates its structural characteristics, physicochemical properties, and inhibitory effects of Michigan Cancer Foundation-7 (MCF-7) breast cancer cells. The influence of processing conditions on nanoparticle size and stability at 25 °C was assessed using particle size and zeta potential measurements. The Se-TFPs were synthesized by optimizing the processing conditions via response surface methodology, yielding nanoparticles with a selenium (Se)-to-polysaccharide mass ratio of 1:13.5, a Se-to-ascorbic acid molar ratio of 1:4.5, a selenite concentration of 10.7 mM, and a reaction time of 4.4 h. The resulting Se-TFPs had an average particle size of 107.2 nm and a zeta potential of -35.1 mV. Structural and physicochemical analyses confirmed successful nanoparticle formation. Compared to TFPs, Se-TFPs exhibited significantly enhanced scavenging activity against 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), hydroxyl radicals, and superoxide anion radicals. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays, flow cytometry, and cell cycle apoptosis analysis revealed that Se-TFPs effectively inhibited MCF-7 cell proliferation at the S phase, with an IC50 value of 119.62 μg/mL.
研究领域:
纳米技术
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药理学
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肿瘤学
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生物化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceuticals
·
2024
·Vol. 17
·期 10
·1273
·
IF 4.3
Bo Yang,Xu Zhang,Liguo Zhang,Jinjin Wu,Wei Wang,Qiaomei Huang,Zhenyuan Wang,Jichuan Zhang,Tongjie Xu,Chengyu Wu,Jiaheng Zhang
Objectives: To address the poor efficacy and percutaneous penetration of grape seed oil, ionic liquids and nanotechnology were combined to prepare a grape seed oil emulsion. Methods: A novel Menthol-CoQ10 ionic liquid and ionic liquid based grapeseed oil emulsion were prepared and confirmed. Results: The average size of the grapeseed oil emulsion was 218 nm, and its zeta potential was −33.5 mV. The ionic liquid-based grape seed oil emulsion exhibited a transdermal penetration effect 4.63-fold higher than that of ordinary grape seed oil emulsion. Ionic liquid also displayed enhanced efficiency both in vitro and in vivo. It significantly inhibited the production of DPPH free radicals and tyrosinase, inhibited melanin and matrix metalloproteinase-1 (MMP-1) produced by cells, and promoted type I collagen expression in fibroblasts. After 28 days of continuous use, the grapeseed oil emulsion improved the water content of the stratum corneum and the rate of transepidermal water loss, enhanced the firmness and elasticity of the skin, and significantly improved the total number and length of under-eye lines, tail lines, nasolabial folds, and marionette lines on the face. Conclusions: Menthol-CoQ10 ionic liquid is a promising functional excipient for both transdermal delivery increase and efficient enhancement. Ionic liquid and nanotechnology for grape seed oil facial mask displayed significantly enhanced efficacy and permeability.
研究领域:
药学
·
化妆品科学
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纳米技术
·
皮肤病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2022
·
IF 7.425
Nan Sun,Jiayan Xie,Jianhua Xie,Yi Chen,Xiaobo Hu,Qiang Yu
In this study, the static adsorption–desorption performance of seven macroporous resins for bound polyphenols from mung bean coat dietary fiber (MBDF-BP) was compared, and NKA-9 macroporous resin was preferably screened for subsequent separation and purification. The composition of the purified products was identified and quantified, 44 major compounds were detected, with the main phenolic acid being p -hydroxybenzoic acid, which contained up to 8881.90 μg/g DW. The purification enriched flavonoids, with high contents of catechin (1419.03 μg/g DW) and vitexin (615.88 μg/g DW). The MBDF-BP purified products (pMBDF-BP) produced significant reversible inhibitory activity against α -glucosidase in a mixed-type inhibition manner, which was superior to the reported crude extracts. The antioxidant activity assays showed that pMBDF-BP exhibited distinct scavenging effects on DPPH•, ABTS + •, •OH free radicals, as well as reactive oxygen species (ROS) in Caenorhabditis elegans ( C. elegans ). These results demonstrated that NKA-9 macroporous resin could effectively enrich MBDF-BP extracts and enhance its antioxidant activity, which was promising to explore new sights into the applications of bound polyphenols from mung bean coat dietary fiber in functional foods or dietary supplements, thus contributing to the scientific utilization of mung bean coat resources and increasing the added value of related products.
研究领域:
食品科学
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植物化学
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生物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2021
·
IF 4.36
Chunlian Li,Yijie Luo,Weicheng Zhang,Qiuyang Cai,Xianyi Wu,Zekai Tan,Rui Chen,Zehao Chen,Shimin Wang,Lanyue Zhang
Ethnopharmacological relevance Essential oils (EOs) are important active ingredients in Chinese herbal medicine. Cymbopogon citratus (DC.) Stapf, Cinnamomum cassia (L.) Presl, Salvia japonica Thunb., and Rosa rugosa Thunb. are used in local and traditional medicine to treat inflammation-related diseases. While the constituents and bioactivities of EOs of the above four Chinese herbals have been reported previously, but their anti-inflammatory and antioxidant activities have not been systematically evaluated. Aim of the study The purpose of this study was to research the chemical constituents, anti-inflammatory activities, and antioxidant activities of EOs of four Chinese herbal medicines. Materials and methods Fresh plant materials were collected both in August 2018. The compositions of EOs were determined by the gas chromatography-mass spectrometry (GC-MS) method. TPA (12-O-tetradecylphophenol-13-acetate)-induced mouse ear model was evaluated the anti-inflammatory activities of EOs. The reduction of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), cyclooxygenase-2 (COX-2) and RelA protein of the nuclear factor kappa-B (NF-κB) in the signal pathway (P65) were measured. The antioxidant activities of EOs from four plants were determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Results A total of 217 compounds were separated and identified from four EOs, mainly including trans-cinnamylaldehyde (68.75%), citronellal (38.16%), linalool (1.02–33.73%), geraniol (19.39%) and citronellol (17.18%). All four EOs reduced the damage of the ear tissue and had certain anti-inflammatory effects, and the EOs of C. citratus (CyCEOs) and S. japonica (SJEOs) had the best anti-inflammatory activities among others, even better than ibuprofen . The four EOs exhibited different DPPH radical-scavenging activities (IC 50 , 0.101–1.017%), where most of them were much lower than that of EOs of C. citratus (CiCEOs) (IC 50 , 0.101%) and EOs of R. rugosa (RREOs) (IC 50 , 0.142%). Conclusions The main components determine diverse propertise of these four EOs, our results suggested that four EOs presented anti-inflammatory and antioxidant effect via reducing the expression of TNF-a, IL-6, COX-2, and NF-κB p65, which may provide a new approach for development of new anti-inflammatory drugs.
研究领域:
药理学
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民族药理学
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化学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Shuqi Guo,Xueze Zhang,Shangxuan Li,Huifang Wang,Xianghua Gao,Baolong Niu,Wenfeng Li
Quercetin (Que) is a naturally occurring bioactive compound with various potential health benefits, but its low stability and low bioaccessibility limit its application in functional foods. Therefore, there is an urgent need to prepare a novel composite hydrogel bead with efficient and controlled release. Specifically, porous starch (PS) was first prepared by enzymatic hydrolysis to increase the specific surface area and thus enhance the adsorption rate of Que. Scanning electron microscopy confirmed the formation of the PS and the adsorption of Que. The maximum swelling properties of carboxymethylcellulose-porous starch composite hydrogel beads (CQPZ) were reduced by adding different amounts of zinc oxide to achieve slow release. The results showed that the higher the amount of zinc oxide, the stronger the slow-release performance, while the lower the drug loading capacity. To obtain composite hydrogel beads with both low swelling and high drug-carrying properties, bilayer gel beads (CQPZ/CS) were prepared by electrostatic self-assembly of carboxymethyl cellulose and chitosan. The experimental results showed that the drug loading was significantly increased by nearly 2-fold while enhancing the slow-release performance. In addition, the CQPZ/CS composite hydrogel beads enhanced the stability of Que under light and heat conditions, and the DPPH radical scavenging rate after UV irradiation was still greater than 75%, which was much higher than that of free quercetin (31.08%). In conclusion, the prepared hydrogel beads are a promising delivery system for bioactive compounds.
研究领域:
食品科学
·
药剂学
·
材料科学
·
生物技术
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD PROCESSING AND PRESERVATION
·
2020
·Vol. 45
·期 1
·e15020
·
IF 1.405
Yue Chen,Likang Qin,Anyan Wen,Muhammad Mazhar,Hezhu Wang,Yong Zhu
A three-solvent extracting method, which consist of 80% acetone, ethyl acetate, and 70% ethanol extracting procedures, was adopted to evaluate phenolics profile and antioxidant activities of Tartary buckwheat. Phenolics profile of Tartary buckwheat was determined using HPLC. Gallic acid, chlorogenic acid, and 2,3,4-trihydroxybenzoic acid were detected in 80% acetone extracts, 2,3,4-trihydroxybenzoic acid was detected in ethyl acetate extracts, rutin and quercetin were detected in 70% ethanol extracts, rutin was the dominant phenolic compound, and the contents ranged from 1,902.0 to 2,402.8 mg/100 g DW. Antioxidant activities of Tartary buckwheat were determined using DPPH, ABTS, and FRAP assays, 70% ethanol extracts showed a higher antioxidant activity than 80% acetone and ethyl acetate, respectively. These results suggested that the three-solvent extracting method is more comprehensive than independent one in evaluating phenolics profile and antioxidant activities of Tartary buckwheat. Practical applications The three-solvent extracting method can be used to evaluate functional properties of Tartary buckwheat.
研究领域:
食品科学
·
植物化学
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Yaqi Tu,Yan Xu,Fazheng Ren,Hao Zhang
The characteristics and antioxidant activity of Maillard reaction products (MRPs) from 2′-fucosyllactose (2′-FL), a human milk oligosaccharide, and α-lactalbumin (α-LA) were investigated. MRPs were prepared by reacting 2′-FL with α-LA at 60 °C and 79% relative humidity for up to 72 h. The absorbance and fluorescence intensity of heated α-LA-2′-FL increased as the heating time increased, while the free amino group content decreased, confirming that 2′-FL reacted with α-LA and produced various MRPs at different incubation stages. Conjugates of 2′-FL and α-LA and aggregation of α-LA were observed by SDS-PAGE. The secondary structure of α-LA did not change significantly after reacting with 2′-FL. In terms of antioxidant activity, the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and reducing power of α-LA-2′-FL increased significantly when compared with the protein only sample ( p < 0.05). The findings provide a foundation for the characterization and functional analysis of MRPs in dairy products.
研究领域:
食品科学
·
营养学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2022
·Vol. 12
·期 16
·9744-9754
·
IF 4.036
Xuejing Liu,Xiaowei Chen,Hao Zhang,Shangde Sun
Caffeic acid (CA) is widely found in nature, and has a broad spectrum of biological activities. However, the low hydrophilicity and lipophilicity of CA limited its application. Dodecyl caffeate (DC) is the lipophilic ester of caffeic acid (CA), and also has high antioxidant activity. In this work, CA, used as a substrate, and three ionic liquids with different acidities and H2SO4 were used as economic catalysts for DC preparation. The effects of variables on DC yield were investigated and optimized by response surface methodology (RSM). And the kinetic and thermodynamic parameters of the esterification of CA and dodecanol were evaluated. Results showed that lipophilic DC was successfully synthesized using ionic liquid ([Hnmp]HSO4) as a catalyst. And the optimal conditions by RSM were substrate ratio of 10.2 : 1, IL dosage of 9.8% at 87 °C for 118 min. Under the optional conditions, the maximum DC yield was 94.67 ± 1.32%. The k0, Ea, ΔH, ΔS, and ΔG were 7.18 × 107 mol (L min)−1, 65.77 kJ mol−1, 63.10 kJ (mol K)−1, −103.80 J (mol K)−1, and 99.78 kJ mol−1 at 363 K, respectively. DC prepared in this work showed a good DPPH radical scavenging activity, which indicated that DC can be used as a potential antioxidant in food and cosmetics.
研究领域:
化学
·
材料科学
·
食品科学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 3
·e202200813
·
IF 2.9
Kaiwen Li,Jie Wang,Zizhen Huang,Chunxia Tan
Angelica Sinensis polysaccharide cerium (ASP−Ce) was prepared by Angelica Sinensis polysaccharide (ASP) and cerium ammonium nitrate (NH 4 ) 2 Ce(NO 3 ) 6 . and its morphology, and solid structure was investigated. The antioxidant activity of the ASP−Ce complex in vitro was evaluated. The antioxidant activity of ASP−Ce complex in vitro was evaluated by the scavenging activity of 2,2-diphenyl-1-picrylhyrazyl radical (DPPH), hydroxyl radical (⋅OH) and superoxide anion radical ( ⋅). The results showed that the ASP−Ce had the more ordered structure for inserting the Ce 4+ into the polymer chain of ASP and there was little change in the conformation of the polysaccharide from Ce 4+ . Three free radical scavenging experiments proved that ASP−Ce had better antioxidant capacity than of ASP, especially on DPPH, and then on ⋅. The scavenging rate of ASP−Ce at 1.0 mg/mL on DPPH reached 71.6 %. Therefore, these results provide references for the further development and utilization of rare earth-polysaccharide.
研究领域:
药理学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2023
·
IF 11.2
Jun Ye,Luying Liu,Wu Lan,Jian Xiong
Carboxymethyl cellulose (CMC)/polyvinyl alcohol (PVA) hydrogels loaded with soybean peptide (SPE) were fabricated via a freeze-thaw method. These hydrogels conquer barriers in simulated gastric fluid (SGF), and then release SPE in simulated intestinal fluid (SIF). The results of in vitro SPE release from these hydrogels showed that in SGF only a little of the SPE released, but in SIF the SPE was completely released. The released SPE had scavenging rates for DPPH and ABTS free radicals of 41.68 and 31.43 %. The pharmacokinetic model of SPE release from the hydrogels in SIF was studied. When the hydrogels are moved from SGF to SIF, the sorption of the shrinkage hydrogel network is entirely controlled by stress-induced relaxations. There are swollen and shrunken regions during SPE release. For SPE release into the SIF, SPE has to be freed from the weak bonds in the swollen regions by changes in the conformation of CMC and PVA. The release rate of SPE was found to be governed by the diffusion and swelling rate of the shrinkage hydrogel network. The Korsmeyer-Peppas equation diffusion exponents (n) for SPE release from the hydrogels are >2.063, indicating a super case II transport. These data demonstrate CMC/PVA hydrogels have potential applications in oral peptide delivery.
研究领域:
药学
·
材料科学
·
生物技术
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）