APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2022
·Vol. 128
·期 11
·1-11
·
IF 2.983
Zhang Liang,Zhang Ran,Cui Lili,Li Tao,Gu Dayong,He Jian’an,Zhang Sixiang
The sensitization effect of graphene oxide (GO) on surface plasmon resonance (SPR) chip sensors has been widely reported. However, the absence of a fixed value for selecting the GO solution concentration is a significant limitation for reproducing the experiments reported in the literature. In this study, linear functionalized polyethylene glycol (SH-PEG-NH 2 ) is used to modify the surfaces of bare gold chips, using flow and immersion methods to produce GO-film-covered SPR sensor chips. Deionized water and phosphate buffered saline are applied as refractive index variants to compare the changes in sensor performance following chip modification, and to determine the optimal concentrations of GO solution under the respective modification methods. Using a sensor chip modified with the optimal GO concentration enhances the ability of a 10-year-old device to detect the dengue virus (DENV) E-protein by 357% and enables the detection of antibody protein concentrations as low as 62.5 ng/mL. Satisfactory results were also obtained using a DENV-positive serum to verify the chip usage. The results of this study are of significance for future efforts involving the rapid preparation of accurate and low-cost GO-SPR biosensor chips.
研究领域:
生物传感技术
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病毒检测
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材料科学与工程
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产品关联
DOI
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Wei Liang,Jiayu Zheng,Xinyue Liu,Wenqing Zhao,Qian Lin,Temirlan Khamiddolov,Jie Zeng,Haiyan Gao,Wenhao Li
To investigate the role of E-beam treatment on the structure-properties of oxidized starch, this study investigated the influence of E-beam (1, 3, 6 kGy) pretreatment combined with NaClO oxidation (1% and 3%) on the multi-scale structural, physicochemical, and digestive properties of cassava starch. Results showed that E-beam treatment did not affect the starch surface, but the oxidative modification increased granule surface roughness. Also, the synergistic modification preserved starch growth rings, FT-IR patterns and crystal types. Further investigations revealed that E-beam induced starch molecular degradation, leading to decreased molecular weight, depolymerization of long chains, and a loss of short-range order. Moreover, oxidation treatment exacerbated the disruption in starch molecular structure, as evidenced by crystallinity loss, viscosity, and enthalpy reduction. Notably, E-beam induces starch yellowing; however, oxidative modification increases starch whiteness. Additionally, the synergistic modification improved native starch's lower solubility and enhanced the resistant starch content. Results suggest that E-beam pretreatment can enhance oxidative modification by promoting the exposure of active sites of starch molecules without destroying starch structure and can be considered an advanced, green, and efficient pretreatment for modified starch in the future.
研究领域:
食品科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
·
2023
·
IF 8.3
Jiajun Liu,Xionghao Zhang,Junda Li,Yi Feng,Li Feng,Shuai Han,Tingyu Pan,Taiheng Zhang,Shenyu Wu,Zijie Ke,Bingzhi Liu,Huaili Zheng
In this study, an advanced oxidation process with E/Ce(IV) synergistic PMS (E/Ce(IV)/PMS) was established for the efficient removal of Reactive Blue 19 (RB19). The catalytic oxidation performance of different coupling systems was examined and the synergistic effect of E/Ce(IV) with PMS in the system was substantiated. The oxidative removal of RB19 in E/Ce(IV)/PMS was excellent, achieving a removal efficiency of 94.47% and a reasonable power consumption (EE/O value was 3.27 kWh·m −3 ). The effect of pH, current density, Ce(IV) concentration, PMS concentration, initial RB19 concentration and water matrix on the removal efficiency of RB19 were explored. Additionally, quenching and EPR experiments showed that the solution contains different radicals such as SO 4 ·− , HO∙ and 1 O 2 , where 1 O 2 and SO 4 ·− played key roles, but HO∙ just acted a weaker role. Ce ion trapping experiment confirmed that Ce(IV) was involved in the reaction process and played a major role (29.91%). RB19 was subject to three possible degradation pathways, and the intermediate products displayed well biochemical properties. To conclude, the degradation mechanism of RB19 was explored and discussed. In the presence of current, E/Ce(IV)/PMS performed a rapid Ce(IV)/Ce(III) cycle, continuously generating strong catalytic oxidation Ce(IV), The reactive radicals derived from the decomposition of PMS, in conjunction with Ce(IV) and direct electro-oxidation, efficiently destroyed the molecular structure of RB19 and showed an efficient removal rate.
研究领域:
环境科学
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化学工程
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2020
·Vol. 25
·期 3
·654
·
IF 3.267
Dongmei Ke,Wenxue Chen,Weijun Chen,Yong-Huan Yun,Qiuping Zhong,Xiaotang Su,Haiming Chen
Vitamin E (VE) and β-cyclodextrin (β-CD) can form an inclusion complex; however, the inclusion rate is low because of the weak interaction between VE and β-CD. The results of a molecular docking study showed that the oxygen atom in the five-membered ring of octenyl succinic anhydride (OSA) formed a strong hydrogen bond interaction (1.89 Å) with the hydrogen atom in the hydroxyl group of C-6. Therefore, β-CD was modified using OSA to produce octenyl succinic-β-cyclodextrin (OCD). The inclusion complexes were then prepared using OCD with VE. The properties of the inclusion complex were investigated by Fourier-transform infrared spectroscopy (FT-IR),13C CP/MAS NMR, scanning electron microscopy (SEM), and atomic force microscopy (AFM). The results demonstrated that VE had been embedded into the cavity of OCD. Furthermore, the emulsifying properties (particle size distribution, ζ-potential, and creaming index) of the OCD/VE inclusion-complex-stabilized emulsion were compared with that stabilized by β-CD, OCD, and an OCD/VE physical mixture. The results showed that the introduction of the OS group and VE could improve the physical stability of the emulsion. In addition, the OCD/VE inclusion complex showed the strongest ability to protect the oil in the emulsion from oxidation. OCD/VE inclusion complex was able to improve the physical and oxidative stability of the emulsion, which is of great significance to the food industry.Keywords:octenyl succinic β-cyclodextrin;vitamin E;inclusion complex;emulsifying property;oxidation stability
研究领域:
食品科学
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化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Haiyan Li,Zekun Jin,Nengguo Tao
This study aimed to develop food-grade ethyl cellulose/agarose aerogels for the controlled release of plant essential oils to extend the shelf life of fresh rice noodles. The antibacterial efficacy of ten unsaturated aldehyde essential oils was evaluated against primary spoilage bacteria, including Leuconostoc lactis , Lactococcus lactis , Acinetobacter johnsonii , Exiguobacterium indicum , and Enterobacter sichuanensis . Based on its superior antimicrobial performance, (E)-2-hexenal was selected for encapsulation within ethyl cellulose/agarose aerogels to achieve controlled release and extend the shelf life of fresh rice noodles. Increasing the ethyl cellulose concentration resulted in a more uniform pore size distribution, enhanced mechanical properties and hydrophobicity, and improved controlled release of (E)-2-hexenal in the aerogels. The optimized aerogel, loaded with 0.20 μL/mL of (E)-2-hexenal, exhibited significant antimicrobial activity, effectively inhibiting bacterial growth for 72 h. Moreover, the aerogel formulation effectively preserved the quality of fresh rice noodles by mitigating changes in total plate count, pH, water content, water activity, cooking properties, color, and texture, ultimately extending the product's shelf life by 4 days at 25 °C. These findings highlight the potential of ethyl cellulose/agarose aerogels loaded with (E)-2-hexenal as an innovative preservation strategy for fresh rice noodles, providing a promising method for enhancing food quality and longevity.
研究领域:
食品科学
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微生物学
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产品关联
DOI
PubMed
高影响因子
Coatings
·
2023
·Vol. 13
·期 5
·829
·
IF 3.4
Rundong Wang,Shuangjiang Feng,Yanyun Wang,Chengqian Li,Xiaohai Bu,Yuzhong Huang,Man He,Yuming Zhou
Electronic skin (E-skin) is increasingly utilized in modern society, yet current E-skin technology suffers from issues, such as opacity, hardness, and fragility. To address these challenges, a novel E-skin was developed using polyurethane (PU) as the matrix material and silver nanowires (AgNWs) as the sensing material. By leveraging the small degree of microphase separation and lack of crystallization in the PU, combined with the appropriate length–diameter ratio of the AgNWs, the resulting E-skin exhibited a visible light transmittance of 75%. The E-skin also showed excellent self-healing properties (83.63% efficiency in the third repair) and mechanical properties (with almost no degradation after 60 tensile cycles) due to the reversible dynamic cross-linking network within the PU. The synergistic effect of PU and AgNWs resulted in exceptional sensing performance for the E-skin, with a gauge factor of 46 (when ε = 10%). Moreover, the E-skin demonstrated signal stability during human joint motion monitoring and successfully identified different movement states, highlighting its potential for diverse applications. This research presents a simple yet effective approach for producing transparent, durable, and stable E-skin.Keywords:electronic-skin (E-skin);strain-sensor;self-healing;polyurethane;AgNWs;spraying;transparent;UV blocking
研究领域:
材料科学
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纳米技术
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生物医学工程
·
电子学
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产品关联
DOI
高影响因子
PLoS One
·
2025
·Vol. 20
·期 5
·e0324164
·
IF 2.6
Bingbing Cao,Jiali Zhou,Bo Xia,Xiaoqing Li,Rui Wang,Yiqiao Xu,Chunqi Li
Choline has been proven to be effective in maintaining liver function. However, the effect of choline, in combination with other nutrients, on the improvement of non-alcoholic fatty liver disease (NAFLD) remains unclear. This study aimed to investigate the potential effect of the nutraceutical complex containing choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate on NAFLD in the zebrafish model. The NAFLD model was induced in zebrafish by administering thioacetamide. Experimental groups were established, including a normal control group, the model control group, the positive control group, the nutraceutical complex intervention group, and the choline bitartrate alone intervention group. The intervention was administered to the zebrafish in a water-soluble form, while the positive control group received polyene phosphatidylcholine at a concentration of 50.0 μg/mL. Notably, the protective effect of the nutraceutical complex against NAFLD is more pronounced than that observed with choline bitartrate supplementation alone. The results of transcriptomics and quantitative real-time PCR showed that the potential mechanisms underlying the effects of the nutraceutical complex might involve the upregulation of acacia, acsl1a , fbp2 gene expression, and the downregulation of tbc1d1 gene expression. These results were further validated by western blotting and overexpression experiments. Our findings indicated that choline bitartrate, zinc citrate, and dl-α-Tocopheryl acetate can help improve NAFLD. The results of this study provide evidence for the application of the nutraceutical complex in the improvement of NAFLD.
研究领域:
营养学
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肝脏病理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Xi-Ling Li,Chengqiang Han,Miao Luo,Shuyun Xiao,Jing Li,Chenglong Yu,Shengyu Cheng,Yueying Jin,Yu Han,Kenichiro Todoroki,Qing Shi,Jun Zhe Min
Glycans play important roles in the activity and function of monoclonal antibodies (mAbs). In this study, an isotope labeling method for the relative quantitative analysis of glycans in cetuximab, a chimeric human/mouse IgG1 monoclonal antibody that specifically targets epidermal growth factor receptor, via hydrophilic interaction LC-ultra-high-performance LC-HRMS was established based on Pronase E digestion. To this aim, novel isotope MS probes, i.e., 3-benzoyl-2-oxothiazolidine-4-carboxylic acid (d0-BOTC) and 3-(2,3,4,5,6-pentadeuterio-benzoyl)-2-oxothiazolidine-4-carboxylate acid (d5-BOTC), which include a carboxyl group to target the amino functional group in glycosylamine, were developed. The nonspecific Pronase E enzyme could simultaneously digest the peptide bound to the N - and O -glycans into glycosylamine having only one amino acid. Since the mass difference between the light- and heavy-labeled glycans was 5.0 Da, the relative abundance of their MS peaks was used to achieve the qualitative and relative quantitative analysis of glycans. Sialylglycopeptide was used as a complex glycan model to validate the accuracy of the method. The results demonstrated the good linearity (R 2 ≥ 0.9994) between the experimentally detected MS intensity ratios and the theoretical molar ratios of the d0-BOTC to the corresponding d5-BOTC derivatives in the dynamic range of 0.03-10 and 0.03-20 of three orders magnitude for the d5-BOTC/d0-BOTC ratios. The reproducibility was between 0.16% and 10.70%, and the limit of detection was 13 fmol. The feasibility of the relative quantification method was investigated by analyzing the glycan content in cetuximab, finding good consistency between experimental and theoretical molar ratios (5:1, 3:1, 1:1, 1:3, 1:5) of d0/d5-BOTC-labeled glycans. Finally, 13 glycans were successfully identified in cetuximab by applying this method using an in-house Tracefinder database. This study provides a novel strategy for the high throughput analysis, identification, and functional study of glycans in mAbs.
研究领域:
生物化学
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药物分析
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
·
2017
·Vol. 106
·期 5
·1827-1841
·
IF 3.189
Yikun Yang,Chunyi Tong,Juchang Zhong,Ruiying Huang,Weihong Tan,Zhikai Tan
Magnetic hyperthermia has been rapidly developed as a potential cancer treatment in recent years. Artificially induced hyperthermia close to a tumor can raise the temperature to 45°C causing tumor cell death. Herein, we introduce a novel method for rapid preparation of anti-cancer magnetocaloric PCL/Fe 3 O 4 mats capable of high-performance hyperthermia using E-jet 3D printing technology. Our 3D printed mats not only maintained the heating efficiency of traditional techniques for magnetic hyperthermia but also prolonged the effective therapy in vivo . When Fe 3 O 4 nanoparticles (NPs) were used in mats at a concentration of 6 mmol/L, 0.07 g PCL/Fe 3 O 4 mats were able to increase the temperature peripherally to 45°C under an alternating magnetic field (AMF) within 45 min. Moreover, the reproducibility experiment indicated that the maximum temperature was achieved following repeated heating and cooling cycles. Cell toxicity tests showed a high cell death rate during one treatment cycle. In vivo experiments indicated clear signs of tumor growth inhibitory and prolonged survival time of tumor-bearing mice after 4 weeks of treatment. The present magnetic mats may be a potential candidate for a novel heat-generating substrate for localized hyperthermia cancer therapy. Furthermore, the main advantage of such implantable magnetic mats is the local and precise delivery of Fe 3 O 4 NPs, ideal for the hyperthermia treatment of easily accessible tumors. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1827–1841, 2018.
研究领域:
生物医学工程
·
材料科学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Journal of Applied Spectroscopy
·
2022
·Vol. 89
·期 3
·559-566
·
IF 0.816
Gao Ch.,Du H.
Forsythoside E is one of the major secondary metabolites in Forsythia suspensa . The interactions between forsythoside E and two types of cholinesterases, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) were investigated in PBS buffer (pH 7.40) by using multispectroscopic techniques. Forsythoside E increased the fluorescence intensity of AChE but quenched the fluorescence of BChE. Synchronous fluorescence studies showed that forsythoside E mainly acts on tyrosine residues of AChE and tryptophan residues of BChE. It was also proved that the complex between the compound and cholinesterases formed spontaneously at a stoichiometric ratio of 1:1 via multispectral technology. Finally, forsythoside E inhibited the activities of cholinesterases with similar IC 50 values of 1.08 mM for AChE and 0.92 mM for BChE. The results illuminate the details of the interaction between forsythoside E and cholinesterases.
研究领域:
药理学
·
生物化学
·
分子生物学
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DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Wenjing Huang,Zhujun Zeng,Yan Lang,Xia Xiang,Gaofu Qi,Gan Lu,Xiangliang Yang
In recent decades, epidemiological, clinical, and experimental studies have demonstrated that a diet with antioxidant or anti-inflammatory function plays a central role in the prevention of atherosclerosis (AS). The purpose of this study was to explore the effects of Cannabis seed oil (CO) administration on in vitro antioxidant capacity as well as blood lipid profiles, lipid peroxidation, inflammatory response, and endothelial cell integrity. Female ApoE–/– mice were fed a high-cholesterol diet and administrated with CO or phosphate-buffered saline (PBS) and seal oil by gavage for 8 weeks. The results show that CO administration reduced the levels of serum triglycerides and low-density lipoprotein cholesterol at week 6. Additionally, a decrease in serum tumor necrosis factor α and nitric oxide was also observed. Moreover, results from CD31 staining and scanning electron microscopy revealed that CO treatment alleviated the endothelial cell damage and lipid deposition induced by a high-cholesterol diet. The ratio of lesion area to the total aorta area was 19.57% for the CO group, which was lower than the PBS control group (24.67%). Collectively, CO exerted anti-atherosclerotic effects by modulating serum lipid profiles and inflammatory responses and improving endothelial cell integrity and arterial lipid deposition. The results provide a promising preventive strategy for the early progression of AS.
研究领域:
营养学
·
心脏病学
·
药理学
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产品关联
DOI
PubMed
高影响因子
WATER SCIENCE AND TECHNOLOGY
·
2022
·Vol. 85
·期 12
·3357-3369
·
IF 2.43
Xu Anlin,Liu Wanqun,Chu Leping,Zhang Yunhai,He Yide,Zhang Yongjun
The cathode is the key component in the electro-peroxone process (E-Peroxone), which is popularly constructed with carbon materials. This study developed an innovative method to fabricate a cathode with waste-tire carbon (WTC) whose performance was evaluated for the degradation of tinidazole (TNZ), an antibiotic frequently detected in water. It was found that the addition of WTC in the cathode can significantly promote the yield of H 2 O 2 and the current efficiency: around 2.7 times that of commercial carbon black at the same loading. The critical influencing factors were studied, including the current density, ozone concentration, initial pH value, chlorine ions and initial TNZ concentration. The scavenger tests demonstrated the possible involvement of •OH and . Some transformation products of TNZ were identified with UPLC-MS and the degradation pathway was proposed accordingly. These results demonstrated the potential of WTC for developing E-Peroxone cathodes. The cathode with WTC generated more H 2 O 2 than a commercial carbon black cathode. Reaction conditions including current density, O 3 dose, pH values, initial TNZ concentration and electrolyte were studied. The main reactive oxygen species were probably •OH and . Transformation products were identified and a degradation pathway was proposed.
研究领域:
环境工程
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电化学
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水处理
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材料科学
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药物污染
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）