COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
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2022
·
IF 5.518
Zhengwei Xu,Xiaohong Jiang
The purpose of the nano-porous TiO 2 modification on TC26 (Ti-13 Nb-13Zr) alloy is to settle the issue that the surface inertness of titanium alloys restricts bioactivity and osseointegration of artificial implants . Secondary constant current anodization was conducted to realize the efficient fabrication of the TiO 2 composite nano-porous arrays containing the terminal small-diameter nanopores and the upper large-diameter nanotubes . The oxygen bubble mould theory has revealed the anodization mechanism of the TiO 2 nano-porous arrays. The anodization parameters and heat treatment conditions are both able to elicit influence on morphology, chemical composition , surface properties and biocompatibility of the as-fabricated samples. The interaction of MC3TC-E1 mouse pre-osteoblasts with a series of TiO 2 nano-porous arrays fabricated by primary anodization (PA) and secondary anodization (SA) was investigated by the MTT assay and alkaline phosphatase (ALP) activity assay. The response of the seeded MC3TC-E1 pre-osteoblasts to the as-fabricated samples with various nano-scale dimensions has indicated that appropriate nano-porous morphologies and heat treatment conditions have the capacity to augment cell adhesion, growth, differentiation and functionality. Such fabrication route in this research has provided a promising prospect for development of artificial implants.
研究领域:
材料科学
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生物材料
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生物医学工程
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细胞生物学
✓ 已验证
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2020
·
IF 3.83
P. Ning,C.C. Liu,Y.J. Wang,X.Z. Li,R. Ranjithkumar,Z.H. Gan,Y.Y. Wu,T. Fu
Magnetic MnFe 2 O 4 nanoparticles containing 0, 1 and 3 at.% silver, respectively were synthesized by one-pot sol-gel method for antibacterial applications in biomedical fields. Material characterizations indicate that MnFe 2 O 4 begins crystallization at 134 °C and oxidation at 450 °C, the grain size and agglomeration degree increase with the silver content and silver exists as metallic state for the particles. The saturation magnetization decreases with the sintering temperature and slightly increases with the silver content, with the maximum of 50.0 emu/g obtained. Antibacterial tests by plate counting and PI-Hoechst 33342 staining suggest that the antibacterial activity of Ag–MnFe 2 O 4 nanoparticles is silver content-dependent. Silver ions concentration measurement, β-galactosidase activity assay and transmission electron microscopic observation show that the antibacterial activity is dominated by the actions of the released silver ions, rather than the membrane cell impairment or reactive oxygen species-induced oxidative stress mechanism. MC3T3-E1 cell test demonstrates the best cytocompatibility of the nanoparticles with 3 at.% silver, which is likely related to the reduced cell endocytosis of the aggregated particles. The combination of magnetism , antibacterial activity and biocompatibility would make Ag–MnFe 2 O 4 nanoparticles a potential multi-functional material in various biomedical applications.
研究领域:
材料科学
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纳米技术
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生物医学工程
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微生物学
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2018
·
IF 3.789
Kuan Zhang,Yi Wang,Tao Sun,Bo Wang,Hongyu Zhang
Electrospun nanofibers, with a typical interconnected porous structure mimicking the extracellular matrix, are commonly used in bone tissue engineering. However, to the best of our knowledge, few studies have been reported to investigate the enhancement of osteogenesis capability of electrospun polycaprolactone (PCL) nanofibers based on bioinspired surface functionalization. In this study, a universal and versatile approach was proposed to spontaneously modify the electrospun PCL nanofibers with bioactive nano-hydroxyapatite (nHA), using dopamine as an effective bioadhesive agent. The evaluation of scanning electron microscopy, energy-dispersive spectroscopy, Fourier transform infrared spectroscopy, thermogravimetric analysis, and surface wettability indicated that nHA was successfully coated onto electrospun nanofibers (PCL-PDHA). Furthermore, in vitro cell experiment including adhesion, proliferation, and osteogenic capability and in vitro biomineralization test in simulated body fluid revealed that the PCL-PDHA nanofibers were biocompatible to MC3T3-E1 cells, and the osteogenesis and biomineralization capabilities were greatly improved in comparison with that of PCL nanofibers. In summary, the facile bioinspired surface functionalization method introduced in the present study, due to its universality and versatility, not only can be used to improve osteogenesis of electrospun nanofibers but also can be regarded as an avenue to achieve other predesigned purposes in biomedical engineering.
研究领域:
生物医学工程
·
组织工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2013
·
IF 2.404
Junmin Qian,Weijun Xu,Xueqing Yong,Xinxia Jin,Wei Zhang
In this study, biomorphic poly( dl -lactic- co -glycolic acid)/nano-hydroxyapatite (PLGA/nHA) composite scaffolds were successfully prepared using cane as a template. The porous morphology, phase, compression characteristics and in vitro biocompatibility of the PLGA/nHA composite scaffolds and biomorphic PLGA scaffolds as control were investigated. The results showed that the biomorphic scaffolds preserved the original honeycomb-like architecture of cane and exhibited a bimodal porous structure. The average channel diameter and micropore size of the PLGA/nHA composite scaffolds were 164 ± 52 μm and 13 ± 8 μm, respectively, with a porosity of 89.3 ± 1.4%. The incorporation of nHA into PLGA decreased the degree of crystallinity of PLGA, and significantly improved the compressive modulus of biomorphic scaffolds. The in vitro biocompatibility evaluation with MC3T3-E1 cells demonstrated that the biomorphic PLGA/nHA composite scaffolds could better support cell attachment, proliferation and differentiation than the biomorphic PLGA scaffolds. The localization depth of MC3T3-E1 cells within the channels of the biomorphic PLGA/nHA composite scaffolds could reach approximately 400 μm. The results suggested that the biomorphic PLGA/nHA composite scaffolds were promising candidates for bone tissue engineering.
研究领域:
生物材料
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组织工程
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APL Materials
·
2024
·Vol. 12
·期 9
·
IF 5.3
Zhang Mingjie,Li Xiaolei,Lin Han
The quality of life is significantly impacted by bone defects, which calls for the creation of optimum restorative materials with particular qualities. Current repair materials, such as metal alloys, polymer scaffolds, and bone cement, have a number of drawbacks, such as poor fracture toughness, non-degradability, and insufficient osteogenic ability. To address these challenges, we designed a novel magnetic casein/CaCO 3 /Fe 3 O 4 microspheres (CCFM), combining biodegradability, osteoinductivity, osteoconductivity, and osteogenesis properties together. In vitro studies confirmed the outstanding biocompatibility and osteogenic differentiation effects on MC3T3-E1 cells of CCFM, highlighting their potential as a promising bone regeneration platform for clinical applications. As a novel bone repair material with superparamagnetic properties, CCFM not only possess good osteoinductivity, osteoconductivity, and osteogenesis properties but also can remain in the lesion location for a long time under an external magnetic field, representing a significant advancement in the field of bone tissue engineering and offering new possibilities for effective bone defect remediation and patient care.
研究领域:
生物材料
·
组织工程
·
生物医学工程
·
骨科
✓ 已验证
产品关联
DOI
高影响因子
LANGMUIR
·
2018
·
IF 3.789
Yongxian Guo,Jie Lu,Qi Kang,Ming Fang,Li Yu
Supramolecular materials assembled by amide-functionalized surface active ionic liquid, N-dodecyl-N′-acetamido imidazolium bromide ([C12ImCONH2]Br), and europium-containing polyoxometalates (Eu-POM) were fabricated in aqueous solution by a one-step method via ionic self-assembly strategy. The [C12ImCONH2]Br/Eu-POM supramolecular structures exhibit favorable fluorescence properties and represent a 15-fold increase in quantum yield (∼13.68%) compared to Eu-POM. Besides, more fluorescence was quenched obviously with the increasing concentration of dopamine (DA) (within the range of 0–100 μM), based on which DA monitoring could be achieved. The detection limit was identified to be 0.1 μM. The supramolecular nanoparticles are highly specific for the detection of DA. In addition, the hybrid assemblies display not only low cytotoxicity but also excellent biocompatibility to MC3T3-E1 cells. As a result, as-prepared supramolecular materials with these superior properties show the promising application in some fields such as biochemistry and biomedical science.
研究领域:
材料科学
·
分析化学
·
生物医学工程
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2019
·
IF 4.959
Honglin Luo,Deqiang Gan,Miguel Gama,Junpin Tu,Fanglian Yao,Quanchao Zhang,Haiyong Ao,Zhiwei Yang,Junjie Li,Yizao Wan
Developing fibrous scaffolds with hierarchical structures that closely mimic natural extracellular matrix (ECM) is highly desirable. However, fabricating scaffolds with true nanofibers (<100 nm) and submicrofibers (<1 μm) remains a big challenge. In this work, to mimic the fibrillar structure of natural ECM, bacterial cellulose (BC) nanofibers were hybridized with cellulose acetate (CA) submicrofibers for the first time. The interpenetrated nano-submicron fibrous BC/CA scaffold was fabricated using the combined electrospinning and modified in situ biosynthesis method. The BC/CA scaffold has an integrated symmetrical nanostructure in which BC nanofibers (42 nm in diameter) penetrate into the submicrofibrous CA (820 nm in diameter) scaffold. The BC/CA scaffold shows an interconnected porous structure with a high porosity of >90%. Additionally, the combination of CA submicrofibers with BC nanofibers leads to significantly improved mechanical properties over nanofibrous BC and submicrofibrous CA scaffolds and enlarged pores over nanofibrous BC scaffold. In addition, the biological behaviors of prepared BC/CA on MC3T3-E1 cells were investigated. Results suggested that BC/CA scaffold is beneficial for cell migration and proliferation. Moreover, the BC/CA scaffold shows higher alkaline phosphatase (ALP) activity, and calcium depositions. In addition, the hierarchical structures can effectively improve the expression of osteogenic gene (ALP mRNA and Runx2 mRNA) and protein (ALP). We believe that the methodology might provide biomimetic morphological microenvironments for enhanced tissue regeneration.
研究领域:
生物材料
·
组织工程
·
纳米技术
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细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Waste and Biomass Valorization
·
2023
·Vol. 15
·期 5
·2625-2635
·
IF 3.2
Luo Xiaoliang,Zhao Bincheng,Yao Junwen,Peng Mingguo,Mao Linqiang,Zhang Wenyi,Shen Rongyan
A large amount of estrogen is introduced into the feed to improve livestock growth, excessive residual estrogen would exist in the excrements. The accumulation of estrogen can cause the water and soil pollution around the farm, further inhibit growth and induce caner for organisms and human. Anaerobic digestion (AD) could effectively remove estrogen in livestock manure while the mechanism is still unclear. In this study, the mechanism of estrogen removal by AD was explored during AD process of pig manure. Estrone (E1), estradiol (E2) estriol (E3) and ethinylestradiol (EE2) were selected as research subjects. The removal rates of E1, E2, E3 and EE2 were 19.14, 28.62, 25.83 and 11.81%. Dissolved organic matters (DOM), especially humic acid, play an important role in reducing bioavailability of estrogen. Estrogen will be absorbed by DOM through structures such as aromatic ring and amides. Estrogen may also promote the growth of microorganisms which could degrade estrogen, such as Rhodococcus , Sphingomonas and Pseudomonas . The amount of these microorganisms and dissolved microbial metabolites did not change obviously. This study would give a new explanation for the removal of estrogen and can provide theoretical support for harmless treatment of pig manure. Graphical
研究领域:
环境科学
·
微生物学
·
生物技术
·
生态毒理学
·
废弃物管理
✓ 已验证
DOI
高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Hongli Zheng,Qingrong Zhang,Zhensheng Hong,Yanyu Lin,Hong Dai
Prostaglandin E1, a cardiac biomarker, is a pivotal member of the prostaglandin family which exerts diverse and powerful effects on cell function in many organ systems. In this study, a new approach for the ultrasensitive determination of prostaglandin E1 was proposed by using the ECL of peroxydisulfate with oxygen as coreactant. Compared to most peroxydisulfate ECL sensor, this proposed ECL strategy propounded employing oxygen evolution reaction to enhance the concentration of oxygen, which was unprecedented in ECL sensing platform. Herein, ultrathin NiCo 2 O 4 nanosheets with large surface area and mesoporous structure were prepared by a facile method, and adamantane as enhancer was decorated on the surface of amino-modified NiCo 2 O 4 to generate a bifunctional catalyst of NiCo 2 O 4 @AD(Prostaglandin E), which possessed excellent catalytic performance toward the oxygen evolution reaction. Accordingly, the sensitivity of ECL immunosensor was enhanced with the increasing concentration of oxygen by the bifunctional catalyst of NiCo 2 O 4 @AD. And the ECL response of the peroxydisulfate would decrease owing to the interaction between antibody and antigen on the modified electrode . Consequently, on the basis of the ECL intensity change of peroxydisulfate, the proposed immunosensor has realized ultrasensitive PGE1 assay with a wide linear detection range of 0.1 fg/ml to1 ng/ml and a detection limit of 0.1 fg/ml. The proposed ECL immunosensor was performed to analyze prostaglandin E1 in human serum, and satisfactory recoveries were obtained, indicating its potential applications in clinical diagnostics.
研究领域:
分析化学
·
生物化学
·
材料科学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2018
·
IF 3.997
Shengnan Wang,Yuanyi Yang,Yunfei Li,Jiacheng Shi,Jianshu Zhou,Li Zhang,Yi Deng,Weizhong Yang
Polyetheretherketone (PEEK)-based implants have become popular in hard tissue orthopedic and dental field. However, its inherent bio-inertness limited its applications for bone repair/substitution of osteoporosis patients, with poor osteogenesis capability. In order to ameliorate their bioactivity, the 3D porous PEEK substrate was created by sulfonate processing, and the substrate was subsequently incorporated with strontium (Sr) through a hydrothermal reaction in Sr(OH) 2 solutions. The adiponectin (APN) protein membrane was deposited on the substrate via polydopamine-assisted deposition. Surface characterization results disclosed that the nanostructures had been formed on sPEEK-Sr-APN surafces, and APN coatings on the substrates could adjust Sr release rate and further mediate cell-material interactions. in vitro experiments indicated that the cellular effects (proliferation and differentiation) of MC3T3-E1 were significantly increased with Sr/APN coordinated regulation. This study provides bioactive Sr and APN as promising active components for bio-functional bone regeneration/substitution, and optimizes the osteointegration of PEEK implants in clinic.
研究领域:
生物材料
·
骨科
·
牙科科学
·
生物医学工程
·
组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE & TECHNOLOGY
·
2020
·
IF 6.155
Hui Liu,Rui Liu,Ihsan Ullah,Shuyuan Zhang,Ziqing Sun,Ling Ren,Ke Yang
Implant-related infection and early bone integration are the main risk factors of implants for long-term service, to overcome these difficulties, SLA-TiCu surface was prepared by sandblasting and large-grits etching (SLA) treatment on a novel antibacterial titanium-copper alloy (TiCu), which is the most prevalent surface treatment with micro/submicron hierarchical structures to titanium-based implants. Effects of SLA-TiCu surface on the adhesion, proliferation, apoptosis and differentiation of MC3T3-E1 cells as well as the antibacterial activity against a common orthopedic pathogen ( Staphylococcus aureus ) were studied. Compared to the following surfaces: sandblasting and large-grits etched pure titanium (SLA-Ti), mechanically ground pure titanium and TiCu alloy (M-Ti and M-TiCu), these results indicated that SLA-TiCu surface obviously enhanced the bone-related gene expressions (alkaline phosphates (ALP), collagen type I (COL I), Runt-related transcription factor 2 (RUN × 2), and osteopontin (OPN)). Moreover, SLA-TiCu surface could maintain a sustainable release of Cu 2+ ions and effectively inhibited the viability of bacteria. This study demonstrated that SLA-TiCu surface possessed multifunctional characteristics of improved osteogenic ability and antibacterial activity, making it promising as a novel implant material for hard tissue repairs such as orthopedics and dental implants .
研究领域:
材料科学
·
生物材料
·
骨科
·
牙科
✓ 已验证
产品关联
DOI
高影响因子
Nano Research
·
2024
·
IF 9.5
Wang Aoao,Ma Xinbo,Yang Yafeng,Shi Guoliang,Han Liwei,Hu Xiantong,Shi Rui,Yan Jun,Guo Quanyi,Zhao Yantao
The initial healing stages of bone fracture is a complex physiological process involving a series of spatially and temporally overlapping events, including pathogen clearance, immunological modulation, and osteogenesis. In this study, we have developed a piezoelectric and aligned nanofibrous scaffold composed of ZnO@PCL/PVDF with multiple antibacterial, immunomodulatory, and osteogenic effects using electrospinning technology. This scaffold’s piezoelectric signal output under ultrasound (US) control can be similar to the physiological electrical signals of healthy bone tissue, creating a truly biomimetic electrical microenvironment in the bone defect. In vitro studies have shown that ZnO@PCL/PVDF scaffold significantly enhances the proliferation, migration, and osteogenic differentiation of MC3T3-E1 cells under piezoelectric drive provided by ultrasound. Furthermore, the scaffold exhibits inhibitory effects on the growth of E. coli and S. aureus , as well as the ability to induce M2 macrophage polarization, indicating potent antibacterial and immunomodulatory properties. In vivo experiments demonstrated that the ZnO@PCL/PVDF scaffold can accelerate the repair of mandibular defects in rats, effectively inhibit bacterial colonization, and reduce inflammatory responses. Altogether, this study confirms that the newly developed ZnO@PCL/PVDF scaffold effectively promotes bone repair by truly mimicking the endogenous electrical microenvironment and precisely regulating the temporospatial disorders of initial bone healing, thus providing a simple and effective solution for bone defects.
研究领域:
生物医学工程
·
材料科学
·
再生医学
·
生物材料
·
骨科
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）