International Journal of Nanomedicine
·
2024
·
IF 6.6
Zixia Wu,Weibo Xia,Liling Ou,Ling Zheng,Bingying Hou,Tonghe Pan,Wenjie Sun,Leo H Koole,Yongqing Shao,Lei Qi
Purpose Patients afflicted with dry eye disease (DED) experience significant discomfort. The underlying cause of DED is the excessive accumulation of ROS on the ocular surface. Here, we investigated the nitrogen doped-graphene quantum dots (NGQDs), known for their ROS-scavenging capabilities, as a treatment for DED.Methods NGQDs were prepared by using citric acid and urea as precursors through hydrothermal method. The antioxidant abilities of NGQDs were evaluated through: scavenging the ROS both extracellular and intracellular, regulating the nuclear factor-erythroid 2-related factor (Nrf2) antioxidant pathway of human corneal epithelial cells (HCECs) and their transcription of inflammation related genes. Furthermore, NGQDs were modified by Arg-Gly-Asp-Ser (RGDS) peptides to obtain RGDS@NGQDs. In vivo, both the NGQDs and RGDS@NGQDs were suspended in 0.1% Pluronic F127 (w/v) and delivered as eye drops in the scopolamine hydrobromide-induced DED mouse model. Preclinical efficacy was compared to the healthy and DPBS treated DED mice.Results These NGQDs demonstrated pronounced antioxidant properties, efficiently neutralizing free radicals and activating the intracellular Nrf2 pathway. In vitro studies revealed that treatment of H2O2-exposed HCECs with NGQDs induced a preservation in cell viability. Additionally, there was a reduction in the transcription of inflammation-associated genes. To prolong the corneal residence time of NGQDs, they were further modified with RGDS peptides and suspended in 0.1% Pluronic F127 (w/v) to create RGDS@NGQDs F127 eye drops. RGDS@NGQDs exhibited superior intracellular antioxidant activity even at low concentrations (10 μg/mL). Subsequent in vivo studies revealed that RGDS@NGQDs F127 eye drops notably mitigated the symptoms of DED mouse model, primarily by reducing ocular ROS levels.Conclusion Our findings underscore the enhanced antioxidant benefits achieved by modifying GQDs through nitrogen doping and RGDS peptide tethering. Importantly, in a mouse model, our novel eye drops formulation effectively ameliorated DED symptoms, thereby representing a novel therapeutic pathway for DED management.
研究领域:
眼科
·
材料科学
·
药学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Desalination and Water Treatment
·
2024
·
IF 1
Jun Yin,Xinan Cai,Meng Chen
Pluronic F127-block-poly(2-hydroxyethyl methacrylate) (PHEMA-b-F127-b-PHEMA) was beforehand synthesized via the RAFT polymerization and used as an additive with polyethersulfone (PES) matrix to fabricate composite membranes by the phase inversion method. During the membrane formation process, the highly hydrophilic PHEMA blocks migrate spontaneously onto the membrane surfaces, which can be verified by the attenuated total reflectance-Fourier transform infrared spectroscopy. As a result, the composite membrane surface porosity and hydrophilicity were enhanced compared with the unmodified PES membrane. More importantly, the water permeability and anti-fouling ability of composite membranes were improved significantly. Furthermore, the PHEMA blocks enriched on the as-made PES composite membrane surface, which acted as an anchor to immobilize the initiating site. Therefore, a lot of functional polymers were effectively grafted onto the PES composite membranes by surface-initiated radical polymerization.
✓ 已验证
DOI
高影响因子
RSC Advances
·
2024
·Vol. 14
·期 39
·28753-28767
·
IF 3.9
Yu Hu,Tian Lan,Ji Li,Lingjun Li,Jizheng Song
Cantharidin (CTD) has been widely used to treat hepatocellular carcinoma (HCC) in clinical practice. However, the current CTD preparations may induce hepatic and renal damage due to their non-specific distribution. Therefore, redox-sensitive polymer Pluronic F127-disulfide bond-poly(D,L-lactide) (F127-SS-PDLA) and active targeting polymer F127-glycyrrhetinic acid (F127-GA) were synthesized to prepare mixed micelles (GA/F127-SS-PDLA/CTD) for effective delivery of CTD. Fourier transform infrared (FTIR) spectroscopy and 1H nuclear magnetic resonance (1H-NMR) spectroscopy were used to verify the successful synthesis of F127-SS-PDLA and F127-GA. During the preparation, this study was the first to screen the density of GA by cellular uptake assay. The results indicated that mixed micelles with 10% and 15% F127-GA (weight fraction) exhibited superior cellular uptake in comparison to micelles with 5% and 20% F127-GA. GA/F127-SS-PDLA/CTD micelles prepared by thin film hydration method demonstrated excellent drug loading capacity for CTD (16.12 ± 0.11%). The particle size and zeta potential of GA/F127-SS-PDLA/CTD micelles were 85.17 ± 1.24 nm and −11.71 ± 0.86 mV, respectively. Hemolysis and stability assay showed that the mixed micelles had good blood compatibility and could remain stable for 30 days at 4 °C. The redox-sensitivity of GA/F127-SS-PDLA/CTD micelles in vitro was verified under reducing conditions through dynamic light scattering (DLS) and an in vitro drug release experiment, which showed obvious particle size variation and rapid drug release ability. In cellular experiments, GA/F127-SS-PDLA/CTD micelles could induce superior cytotoxicity, apoptosis and intracellular reactive oxygen species (ROS) levels compared with free CTD, non-sensitive F127-PDLA/CTD micelles and redox-sensitive F127-SS-PDLA/CTD micelles. The cellular uptake ability of nile red-labeled GA/F127-SS-PDLA micelles, which was evaluated via fluorescent microscope and flow cytometry, indicated that the modification of GA significantly increased micelle uptake in HepG-2 cells. Consequently, GA/F127-SS-PDLA/CTD micelles could be considered as a satisfactory drug administration strategy in the treatment of HCC.
研究领域:
药学
·
生物医学工程
·
肿瘤学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2020
·Vol. 16
·期 10
·1463-1470
·
IF 4.483
Ding Xiangyu,Wang Yuan,Wen Chunmei,Wang Silu,Ruan Qingqing,Li Zhaofeng,Xu Jinzhong,Xu Zheng,Deng Jie
Rhein is a potential anti-inflammatory agent, but its poor water solubility significantly restricts its clinical application. In this study, rhein micelles (RMs) with improved water solubility were fabricated on Pluronic F127 (F-127). Transmission electron microscopy showed that the
as-prepared RMs displayed a mean diameter of approximately 20 nm and a spherical morphology. The encapsulation efficiency of the micelles towards drugs varied from 81.38 ± 4.35% to 24.87 ± 4.32%. The RMs exhibited a burst release during the first 6 h and a following sustained
release up to 96 h with a biphasic drug release pattern as suggested by the drug release assay. Cytotoxicity assessment showed that the RMs caused no change in cell viability at drug concentrations below 40 μM after 24 and 48 h of incubation. In RAW264.7 macrophages, the RMs inhibited the
lipopolysaccharide-induced activation of p65/NF-κB, which in turn suppressed the transcription of its downstream inducible nitric oxide synthase, and cytokine genes such as interleukin-1β and tumor necrosis factor-α . Simultaneously, the RMs led to reduced cytokine secretions,
including cyclooxygenase-2, prostaglandin E2, nitric oxide, and interleukin-6 in a dose-dependent manner. The RMs reported herein may be a promising candidate for developing anti-inflammatory therapeutic formulations.
研究领域:
药学
·
纳米技术
·
炎症研究
✓ 已验证
DOI
PubMed
高影响因子
Frontiers in Pharmacology
·
2019
·
IF 3.845
Zhu Yan-Hua,Ye Ning,Tang Xin-Feng,Khan Malik Ihsanullah,Liu Hong-Liang,Shi Ning,Hang Li-Feng
All-trans retinoic acid (ATRA) is an effective agent that induces differentiation, inhibits cell proliferation, and acts as an anticancer agent. ATRA was successfully conjugated with Pluronic F127 via esterification to enhance its anticancer effects. Pluronic-ATRA showed high cytotoxicity and inhibitory concentrations (IC50) 50% lower than those of ATRA in various breast cancer cell lines (4T1:31.16–8.57 μg/mL; EMT6: 50.48–7.08 μg/mL; MDA-MB-231:37.58–8.99 μg/mL; BT474:25.27–9.09 μg/mL). In combination with chemotherapy, Pluronic-ATRA synergistically enhanced the cytotoxic effects of cisplatin (CDDP). Pluronic-ATRA combined with CDDP effectively suppressed breast tumor growth in vivo. The results of this study demonstrate the potential of Pluronic-ATRA as an anticancer agent that can be used in combination therapy against solid tumors.
研究领域:
药理学
·
肿瘤学
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANIC CHEMISTRY COMMUNICATIONS
·
2024
·
IF 4.4
Henggang Wang,Shan Sun,Jin Yang,Haoyi Sun,Ke Cheng,Xinxin Wang,Si Li,Hengwei Lin
The efficient treatment of many infectious diseases is hindered by uncontrolled bacterial infections and inflammation induced by reactive oxygen species (ROS). Thus, there is an urgent need for the development of multifunctional therapeutic agents that possessing both antibacterial and antioxidant features. In this study, a novel antibacterial and antioxidant agent, Ga 2 Se 3 nanosheets (NSs), were firstly prepared by the liquid-phase exfoliation method. The Ga 2 Se 3 NSs exhibited broad-spectrum sterilization capabilities, even including multidrug-resistant strains. Noteworthily, Ga 2 Se 3 NSs showed a remarkable 800-fold enhancement in antibacterial efficacy compared to the bulk materials, while also demonstrating low drug resistance compared to the traditional antibiotics. Their superior antibacterial performance is primarily attributed to the disruption of basic bacterial metabolism induced by gallium and selenium components, as well as the dimensional engineering of Ga 2 Se 3 NSs. Cellular studies further confirmed the protective effects of pluronic F127 modified Ga 2 Se 3 NSs against oxidative stress damage, preserving cellular functions through ROS scavenging. This study highlights the superior antibacterial and antioxidant properties of Ga 2 Se 3 NSs, which offers an alternative opportunity for addressing the drug resistance issue in treatment of certain special diseases.
研究领域:
纳米技术
·
材料科学
·
生物医学工程
·
药学
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2025
·
IF 12.1
Wenqi She,Chong Shen,Zaifei Xue,Bin Zhang,Guoliang Zhang,Qin Meng
Current hydrogel strain sensors have never been integrated into dynamic organ-on-a-chip (OOC) due to the lack of sensitivity in aqueous cell culture systems. To enhance sensing performance, a novel strain sensor is presented in which the MXene layer is coated on the bottom surface of a pre-stretched anti-swelling hydrogel substrate of di-acrylated Pluronic F127 (F127-DA) and chitosan (CS) for isolation from the cell culture on the top surface. The fabricated strain sensors display high sensitivity (gauge factor of 290.96), a wide sensing range (0–100%), and high repeatability. To demonstrate its application, alveolar epithelial cells are cultivated on the top surface of the hydrogel strain sensor forming alveolar barriers, and then integrated into dynamic lung-on-a-chip (LOC) systems. This system can sensitively monitor normal physiological breathing, pathological inflammation stimulated by lipopolysaccharide (LPS), and alleviated inflammation through drug intervention.
研究领域:
生物医学工程
·
组织工程
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF POWER SOURCES
·
2015
·
IF 6.217
Yang Fan,Pei-Fang Liu,Zong-Wen Zhang,Ying Cui,Yan Zhang
Porous Pt–Cu alloy networks are synthesized through a one-pot hydrothermal process, with ethylene glycol as the reducing agent and the block copolymer Pluronic F127 as structure-directing agent. The structure, porosity and surface chemical state of as-prepared Pt–Cu alloy with different composition are characterized. The formation mechanism of the porous structure is investigated by time sequential experiments. The obtained Pt 53 Cu 47 alloy possesses a unique 3D hierarchical porous network structure assembled by interconnected nanodendrites as building blocks. Because of the high surface area, concave surface topology and open porous structure, the Pt 53 Cu 47 alloy catalyst exhibits enhanced catalytic activity towards methanol and ethanol electro-oxidation in comparison with commercial Pt black and the Pt 73 Cu 27 alloy synthesized following the same process as Pt 53 Cu 47 .
研究领域:
材料科学
·
催化
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2021
·
IF 8.128
Xiaoqing Lei,Lijin Huang,Ke Liu,Lei Ouyang,Qin Shuai,Shenghong Hu
Developing high-performance materials for the efficient removal of pharmaceutical contaminants from polluted water has gained tremendous attention but is still a huge challenge. Herein, various N -doped porous carbons (NPCs) were fabricated via a facile and solvent-free pyrolysis strategy by using a mixture of melamine , hexamethylenetetramine , Pluronic F 127, and ZnCl 2 as precursor. The resulting NPCs featuring large specific surface areas (803–1002 m 2 /g) and high nitrogen content (3.79–5.24%) were used as efficient adsorbent for the capture of ibuprofen (IBP) from water. Due to the combination of abundant mesoporous and microporous structure as well as high nitrogen content, the adsorption equilibrium was achieved within 60 min and the adsorption capacity was calculated to be 113 mg/g. Furthermore, it was found that the adsorption capacity exhibited a good correlation with the nitrogen content. In addition, the adsorption capacity of the resulting NPC was well-maintained even after 4 cycles due to its superior stability. The study is expected to encourage the rational design and synthesis of versatile heteroatom-doped porous carbons for practical application in the field of environmental remediation.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wei Cheng,Huilin Wen,Haixia Shen,Chenwei Fu,Erkuang Miao,Dongbao Li,Xiaoqiang Chen,Chi Hu,Ziyi Yu
Polymer hydrogels are instrumental in cell immobilization for biomanufacturing, yet traditional hydrogel bioreactors often compromise cell viability, function, and mass transfer. This results in uncontrolled microbial proliferation and significant cell leakage, thereby impeding the continuous production of high-value products. To address these challenges, we introduce an innovative liquid microcavity technique employing a blend of Gelatin-methacryloyl (Gel-MA) and Pluronic F127diacrylate (F127-DA) for precision bioprinting, followed by selective enzymatic digestion of gelatin to create microbial habitats. Through tailoring of the hydrogel’s pore structure and integration of diverse networks, our approach facilitates controlled nutrient transfer, efficient waste removal, and stable mechanical support, all while maintaining a leak-free suspension culture of microorganisms. We validated the effectiveness of our method by fabricating yeast-infused Three-dimensional (3D) lattice constructs, successfully achieving continuous fermentation of glucose into ethanol in a flow reactor with minimal efficiency reduction (≥90 % yield) over five cycles. This research not only confronts the spatial limitations inherent in hydrogel-based systems but also demonstrates a foundational potential for revolutionary change in biomanufacturing practices.
研究领域:
生物技术
·
材料科学
·
生物工程
·
微生物学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2023
·
IF 17.1
Xinyu Liu,Zhongxing Chen,Jieyi Bai,Xin Li,Xin Chen,Zheng Li,Hongxian Pan,Siheng Li,Qingyi Gao,Nan Zhao,Aodong Chen,Huilin Xu,Yinuo Wen,Lan Du,Mei Yang,Xingtao Zhou,Jinhai Huang
Uveitis is a complex ocular inflammatory disease with a multifactorial etiology that can result in blindness. Although corticosteroid eye drops are the primary treatment for anterior uveitis, their efficacy is limited by low bioavailability, adverse effects, and a narrow focus on inflammation. In this study, the multifunctional hydrogel eye drops (designated as DCFH) were developed by incorporating the anti-inflammatory agent dexamethasone (DSP) and reactive oxygen species (ROS) scavenger cerium-based metal–organic frameworks (Ce-MOFs) into thermosensitive triblock copolymer F127 for the synergistic treatment against uveitis. The resulting F127 eye drops offer a favorable alternative to ophthalmic solution due to its thermosensitivity, thixotropy, light transmittance, improved ocular bioavailability, and unexpected anti-inflammatory efficacy. Notably, the participation of nanoporous Ce-MOFs, functional drug carriers, not only reduces ROS level but also boosts the anti-inflammatory activity of DSP in vitro. Therapeutically, the multifunctional DCFH exhibits superior efficacy in treating endotoxin-induced uveitis by mitigating the ophthalmic inflammatory reaction, suppressing inflammatory cytokines (e.g., TNF-α, IL-6, and IL-17) and downregulating the expression of iNOS and NLPR3. This synergistic treatment provides a valuable and promising approach for the management of uveitis and other ocular inflammatory conditions.
研究领域:
眼科学
·
药学
·
炎症研究
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Cancer Nanotechnology
·
2020
·Vol. 11
·期 1
·1-17
·
IF 4.7
Pisano S.,Wang X.,Garcia-Parra J.,Gazze A.,Edwards K.,Feltracco V.,Hu Y.,He L.,Gonzalez D.,Francis L. W.,Conlan R. S.,Li C.
Background Amphiphilic block copolymers used as nanomicelle drug carriers can effectively overcome poor drug solubility and specificity issues. Hence, these platforms have a broad applicability in cancer treatment. In this study, Pluronic F127 was used to fabricate nanomicelles containing the histone deacetylase inhibitor SAHA, which has an epigenetic-driven anti-cancer effect in several tumor types. SAHA-loaded nanomicelles were prepared using a thin-film drying method and characterized for size, surface charge, drug content, and drug release properties. Loaded particles were tested for in vitro activity and their effect on cell cycle and markers of cancer progression.Results Following detailed particle characterization, cell proliferation experiments demonstrated that SAHA-loaded nanomicelles more effectively inhibited the growth of HeLa and MCF-7 cell lines compared with free drug formulations. The 30 nm SAHA containing nanoparticles were able to release up to 100% of the encapsulated drug over a 72 h time window. Moreover, gene and protein expression analyses suggested that their cytoreductive effect was achieved through the regulation of p21 and p53 expression. SAHA was also shown to up-regulate E-cadherin expression, potentially influencing tumor migration.Conclusion sThis study highlights the opportunity to exploit pluronic-based nanomicelles for the delivery of compounds that regulate epigenetic processes, thus inhibiting cancer development and progression.
研究领域:
药学
·
癌症生物学
·
纳米技术
·
药物递送系统
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）