RSC Advances
·
2022
·Vol. 12
·期 3
·1807-1812
·
IF 4.036
Xiaoxiao Chen,Pu Li,Gaojun Wu,Zhe Wang,Chaobiao Huang
A convenient, fast, sensitive and highly selective fluorescence sensor for the detection of glutathione (GSH) based on DNA derived bio-dots (DNA bio-dots)/polydopamine (PDA) nanoparticles was constructed. The fluorescent switch of DNA bio-dots was induced to turn off because of fluorescence resonance energy transfer (FRET) reactions between DNA bio-dots and PDA. The presence of GSH blocked the spontaneous oxidative polymerization of dopamine (DA) to PDA, leading the fluorescent switch of DNA bio-dots to be “turned on”. The degree of fluorescence recovery of DNA bio-dots is linearly correlated with the concentration of GSH within the range of 1.00–100 μmol L−1, and the limit of detection (LOD) is 0.31 μmol L−1 (S/N = 3, n = 9). Furthermore, the fluorescence sensor was successfully used to quantify GSH in human urine and glutathione whitening power, indicating the fluorescence sensor has potential in the detection of human body fluids and pharmaceutical preparations.
研究领域:
分析化学
·
生物医学工程
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2026
·
IF 4.8
Yan Tu,Rongjie Yang,Shuli Li,Runhu Huang,Yueye Zhang,Qi Yang,Jinze Li,Lianqun Zhou,Wei Zhang
The microRNAs miR-34a and miR-34b are dysregulated in Alzheimer's disease (AD) and are promising diagnostic biomarkers. However, their low abundance, high sequence homology, and instability present severe challenges for accurate multiplex detection. Herein, we report a quantum dot-enhanced turn-up/down dual-miRNA FRET sensor (QDTF sensor), a platform that achieves simultaneous detection of two highly homologous miRNAs with low crosstalk via an unprecedented asymmetric dual-mode FRET strategy enhanceded by a single quantum dot 605. Among the two pairs of molecular beacons, one triggers a considerable increase in the Alexa Fluor 568 signal for miR-34a, while the other induces a decrease in the Alexa Fluor 532/Cy5 signal for miR-34b. The deliberate opposite-signaling design and spectrally well-separated dye pairs present excellent channel isolation with low crosstalk (<3.42%), even when both targets are present at high concentrations. The QDTF sensor delivers LODs of 0.96 pM (miR-34a) and 1.12 pM (miR-34b) directly in artificial cerebrospinal fluid (aCSF) without enzymes, amplification, or pretreatment, with a dynamic range spanning approximately 1 pM to 10 nM, representing a 322-fold and 203-fold sensitivity enhancement over dye-only molecular beacons, respectively. The QDTF sensor offers a simple, robust, and truly dual approach for the precise quantification of homologous miRNAs in undiluted aCSF, holding immense potential for the early clinical diagnosis of AD.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2015
·Vol. 25
·期 28
·4412-4418
·
IF 11.805
Hongbo Yuan,Junjie Qi,Chengfen Xing,Hailong An,Ruimin Niu,Yong Zhan,Yibing Fan,Wenmin Yan,Ruihua Li,Bing Wang,Shu Wang
The conformation of calmodulin (CaM) changes from closed configuration to open one, converting to a claviform dumbbell-shaped biomolecule upon Ca 2+ -binding. A hybrid probe of graphene oxide (GO) cationic conjugated polymer for detection of the conformation transition of CaM by using FRET technique is demonstrated. The stronger hydrophobic interaction and weaker electrostatic repulsion leads to more CaM adsorption to the surface of GO upon binding with Ca 2+ than that of CaM in the absence of Ca 2+ (apoCaM), resulting in much farther proximity between poly[(9,9-bis(6′- N , N , N -trimethylammonium)hexyl)-fluorenylene phenylene dibromide] (PFP) and green fluorescent protein labeled at the N-terminus of CaM and therefore much weaker FRET efficiency for PFP/Ca 2+ /CaM in comparison with that of PFP/apoCaM in the presence of GO. Notably, the assembly of CaM with GO is quantitatively and reversibly controlled by Ca 2+ ions.
研究领域:
生物化学
·
材料科学
·
分子生物学
✓ 已验证
DOI
高影响因子
POLYMER
·
2022
·
IF 4.432
Yuan Liu,Fei Hu,Shuo Wang,Mengmeng Xu,Qianqian Yu,LinGe Wang
Polymersomes (polymeric vesicles) are non-viral delivery vehicles self-assembled from block copolymers (BCPs), holding promising potential in biomedical applications. Despite the higher chemical stabilities of the BCPs, lyophilization is still required for the long-term preservation of the polymersomes and their encapsulated drugs. However, formulation and processing parameters can affect the efficiency of the lyophilization. In this work, we present an approach to evaluate the integrity of polymersomes based on Fluorescence Resonance Energy Transfer (FRET) for rapid optimization of the lyophilization parameters. Specifically, Coumarin 6 (C6) and Nile red (NR)-encapsulated polymersomes were fabricated separately and mixed together for lyophilization. Upon reconstitution, FRET was detected for polymersomes which lost integrity during the lyophilization or reconstitution. The evaluation was carried out for poly(ethylene glycol)- b -poly(lactic acid) (PEG- b -PLA) polymersomes lyophilized with eight different excipients , and the results showed that the best polymersomes integrity could be achieved with the addition of hydroxypropyl-beta-cyclodextrin (HP-β-CD). We believe the approach described here would greatly facilitate the optimization of the polymersomes lyophilization, accelerating the translation of polymersomes to practical applications.
研究领域:
生物医学工程
·
药物递送系统
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-8
·
IF 4.259
Li Dong-Peng,Wang Zhao-Yang,Cui Jie,Wang Xin,Miao Jun-Ying,Zhao Bao-Xiang
A new ratiometric fluorescent probe was constructed with hemicyanine and 7-nitrobenzofurazan for detection of sulfur dioxide derivatives (HSO 3 − /SO 3 2− ). The ratiometric response mode could be attributed to the efficient FRET (Förster resonance energy transfer) platform. The probe exbihited some desirable properties including fast response (within 2 minutes), good selectivity and high sensitivity. Moreover, the probe could detect endogenous HSO 3 − in liver cancer cells rather than normal liver cells, implying the diagnosal potential of the probe.
研究领域:
生物化学
·
医学诊断
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
·
2022
·
IF 4.117
Tan Qin,Yu Wang,Jing Nie,Lushan Yu,Su Zeng
Background Sodium taurocholate co -transportering polypeptide (NTCP, SLC10A1 ) is a vital bile acid transporter and the functional receptor of hepatitis B and D virus. The oligomerization of NTCP is important for the structural study of its interaction with HBV preS1 peptide. Methods Recombinant NTCPs were expressed in Sf9 host cell using baculoviruses. Function of recombinant NTCP was verified by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method. A quantitative fluorescence resonance energy transfer (FRET) method was established to analyze the interaction between NTCP wild type (WT) and mutants. Co-immunoprecipitation (Co-IP) was used to test the interaction between NTCP variants. Results Sub-cellular location of recombinant NTCPs varies with the modification of NTCP. Bands of monomer, dimer and oligomers were shown in gel analysis of NTCP. Significant FRET was observed between cyan florescence protein (CFP) tagged NTCP and yellow florescence protein (YFP) tagged NTCP. FRET efficiency between CFP- and YFP-NTCP S267F mutants was lower than WT. Co-IP results showed that S267F interacts with WT NTCP when co-expressed in cell. Conclusion Dimer is the predominant form of NTCP expressed in Sf9 when solubilized with detergent. FRET and Co-IP analysis support that NTCP forms oligomers in Sf9 cell. General significance Our results showed that NTCP formed oligomers in Sf9 cell. Meanwhile the FRET analysis of NTCP variants further elucidated the molecular mechanism of NTCP oligomerization.
研究领域:
病毒学
·
分子生物学
·
细胞生物学
✓ 已验证
DOI
PubMed
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 6
·976
·
IF 5.719
Haiyan Qi,Qiuying Li,Jing Jing,Tao Jing,Chuntong Liu,Lixin Qiu,Rokayya Sami,Mahmoud Helal,Khadiga Ahmed Ismail,Amani H. Aljahani
In our study, a unique ratiometric fluorescent sensor for the rapid detection of arginine (Arg) and acetaminophen (AP) was constructed by the integration of blue fluorescent N-CDs and yellowish-green fluorescent calcein. The N-CD/calcein ratiometric fluorescent sensor exhibited dual emission at 435 and 519 nm under the same excitation wavelength of 370 nm, and caused potential Förster resonance energy transfer (FRET) from N-CDs to calcein. When detecting Arg, the blue fluorescence from the N-CDs of the N-CD/calcein sensor was quenched by the interaction of N-CDs and Arg. Then, the fluorescence of our sensor was recovered with the addition of AP, possibly due to the stronger association between AP and Arg, leading to the dissociation of Arg from N-CDs. Meanwhile, we observed an obvious fluorescence change from blue to green, then back to blue, when Arg and AP were added, exhibiting the “on–off–on” pattern. Next, we determined the detection limits of the N-CD/calcein sensor to Arg and AP, which were as low as 0.08 μM and 0.02 μM, respectively. Furthermore, we discovered that the fluorescence changes of the N-CD/calcein sensor were only responsible for Arg and AP. These results suggested its high sensitivity and specificity for Arg and AP detection. In addition, we have successfully achieved its application in bovine serum samples, indicating its practicality. Lastly, the logic gate was generated by the N-CD/calcein sensor and presented its good reversibility. Overall, we have demonstrated that our N-CD/calcein sensor is a powerful sensor to detect Arg and AP and that it has potential applications in biological analysis and imaging.
研究领域:
生物化学
·
分析化学
·
医学检测
✓ 已验证
DOI
高影响因子
DYES AND PIGMENTS
·
2021
·
IF 4.889
Rongqiao Wang,Shibing Chen,Qiaoru Chen,Hongyu Guo,Fafu Yang
abstract Porphyrin with circularly polarized luminescence (CPL) in aggregated state was seldom presented due to the strong aggregation-caused quenching (ACQ) effect at aggregation. This work designed and synthesized a novel porphyrin derivative ( CPL-P ) with good CPL behaviour in aggregated states based on the strong AIE-FRET effect and good chirality transfer of liquid crystalline porphyrin, which was constructed by using porphyrin unit as core with four AIE cyanostilbene groups bearing peripheral chiral alkyl chains. CPL-P exhibited orderly hexagonal columnar mesophase between 62.5 °C and 113.2 °C. It displayed excellent fluorescence emission at 640–760 nm in aggregated states due to the AIE-FRET effect between cyanostilbene unit and porphyrin moiety. Moreover, CPL-P showed the strong circular dichroism and CPL properties in aggregated states based on the effective chiral transfer of the spiral liquid crystalline self-assembly. The |g lum | value for mesophase attained 6.6 × 10 −3 based on the combining effect of AIE-FRET and chiral transfer. This research supplied a new strategy for transferring typical achiral porphyrin with ACQ effect to luminescent molecule with good CPL behaviour in aggregated state.
研究领域:
化学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2018
·
IF 2.891
Sujitraj Sheth,Mengyuan Li,Qijun Song
Due to clinical importance, detection of dopamine by using easy and rapid method is still ongoing challenge. Here we present a simple and quite efficient method for dopamine (DA) detection in alkalescent medium using in situ prepared Ru(II) complex and sodium dodecyl benzyl sulfonate (SDBS) as highly luminescent luminophore. The luminescence enhancement in the Ru(II) complex (Ru-CIP) has been observed in the miceller medium formed by SDBS. The capability to successively quench the luminescence intensity has been tested for variety of molecules and only dopamine as analyte found to be able to quench luminescence effectively. Hence selective quenching of luminescence by dopamine was used as a tool to detect dopamine and two linear concentration ranges has been established from 0.1 μM to 1μM and from 2 μM to 10 μM with limit of detection (LOD) is 6.6 nM (S/N = 3). Spectral evidence showed that luminescence quenching mechanism arose via Forster resonance energy transfer (FRET) among oxidized DA (i.e. DA quinone) and in situ generated Ru-CIP and SDBS assembly. Due to ultra sensitivity and high selectivity of the prescribed (Ru-CIP-SDBS) luminescent probe has a strong potential for practical analytical application in clinical diagnosis.
研究领域:
分析化学
·
临床诊断
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Jinhua Shang,Shanshan Yu,Yingying Chen,Yuhui Gao,Chen Hong,Fengzhe Li,Fuan Wang
Polynucleotide kinase (PNK) shows an in-depth correlationship with DNA repair and metabolism processes. The in situ visualization of intracellular PNK revealed an extremely biological significance in supplementing reliable and quantitative information on its spatiotemporal distribution in live cells. Herein, we developed a versatile cascaded DNA amplification circuit through the integration of catalytic DNA assembly and hybridization chain reaction circuits and realized the accurate evaluation of intracellular PNK activity via the Förster resonance energy transfer (FRET) principle. Initially, without PNK, trigger T was firmly caged in the PNK-recognizing hairpin HT, resulting in no disturbance of the concatenated circuit. However, with the introduction of PNK, the 5′-OH terminal of PNK-addressing HT was phosphorylated, then the phosphorylated HT could be subsequently digested by λ exonuclease (λ Exo) to produce trigger T of the cascaded DNA circuit. As a result, the integrated circuit was stimulated to produce an amplified FRET signal for quantitatively monitoring the activity of PNK. Due to the λ Exo-specific digestion of 5′-phosphate DNA and the high signal gain of the cascade circuit, our proposed strategy enables the sensitive analysis of PNK activity in vitro and in complex biological samples. Furthermore, our PNK-sensing platform was extensively explored in HeLa cells for realizing reliable intracellular PNK imaging and thus showed high potential in the future diagnosis and treatment of kinase-related diseases.
研究领域:
分子生物学
·
生物化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 36
·4466-4472
·
IF 2.596
Yunxia Yuan,Huichun Yu,Yong Yin
A novel sensitive aptasensor for vascular endothelial growth factor-165 (VEGF165) was constructed based on fluorescence resonance energy transfer (FRET) by employing upconversion nanoparticles (UCNPs) and MoS2 nanosheets as the energy donor and acceptor, respectively. The upconversion fluorescence resonance energy transfer (UC-FRET) was triggered by the physical adsorption interaction between the aptamer and MoS2 nanosheets, leading to a remarkable quenching of UCNP fluorescence up to 95%. Upon addition of VEGF165 to the UCNP–aptamer system before MoS2 nanosheets were added, the aptamer preferentially bound to VEGF165 with the change of spatial conformation, which weakened the van der Waals' force between the MoS2 nanosheets and the aptamer, thus leading to the separation of the donor and the acceptor. Consequently, the FRET phenomenon was inhibited and the luminescence of UCNPs was regained, which was linearly related to the concentration of VEGF165 in the range of 0.1 ng mL−1 to 16 ng mL−1. By taking advantage of the extreme fluorescence quenching ability of MoS2 nanosheets and the optical merits of UCNPs, the aptasensor based on UC-FRET exhibited favorable performance for the homogeneous assay of VEGF165 in human serum, which is of great value for clinical diagnosis of tumors and related biological studies.
研究领域:
生物医学工程
·
分析化学
·
纳米技术
·
临床诊断
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Weisheng Xue,Baoshan He,Xiaoyu Cao,Lingling Xie,Yao Liu,Wenhong Zhao,Wenjie Ren,Yiwei Xu
Background Bisphenol A (BPA) is a common monomer in polycarbonate plastics and epoxy resins that can migrate into food and water under extreme conditions, posing significant health risks including endocrine disruption, infertility, and metabolic disorders. Conventional detection techniques such as HPLC, HPLC-MS, and ELISA offer high sensitivity but suffer from costly instrumentation, complex pretreatment, and limited field applicability. Aptamer-based biosensors provide an attractive alternative due to their high specificity, low cost, and ease of use. Dual-mode platforms integrating colorimetric and fluorescent signals can overcome the limitations of single-mode detection Results Here, we developed an electrostatic–FRET coupled dual-mode aptasensor for BPA detection using cysteamine-modified gold nanoparticles (CS-AuNPs) and a FAM-labeled aptamer. In the absence of BPA, FAM-aptamer adsorbed onto CS-AuNPs, leading to fluorescence quenching and colloidal stability. Upon BPA binding, aptamer conformational change suppressed electrostatic adsorption, resulting in fluorescence recovery and nanoparticle aggregation with a visible color shift. Under optimized conditions, the fluorescence mode displayed a linear response from 10–1000 nM with a detection limit of 1.19 nM, while the colorimetric mode showed a range of 25–1000 nM with a detection limit of 2.22 nM. The dual-mode system demonstrated high selectivity against BPA analogues and reliable recoveries (92.7–109.2%) in spiked tap water, confirming its accuracy and robustness in real sample analysis Significance This study presents a cost-effective and visually responsive aptasensor that integrates electrostatic adsorption and FRET for sensitive BPA detection. By combining fluorescence and colorimetric modes, the platform achieves complementary readouts, reducing false positives and enhancing detection reliability. The work highlights the potential of positively charged AuNPs in multimodal biosensing, offering a promising tool for environmental monitoring and food safety assessment.
研究领域:
环境监测
·
食品安全
·
生物传感器
·
纳米技术
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）