PLANT BIOTECHNOLOGY JOURNAL
·
2019
·Vol. 18
·期 7
·1550-1561
·
IF 6.84
Qi Gao,Huifeng Luo,Yongping Li,Zhongchi Liu,Chunying Kang
Summary Fruit colour affects consumer preference and is an important trait for breeding in strawberry. Previously, we isolated the Reduced Anthocyanins in Petioles ( RAP ) gene encoding a glutathione S-transferase (GST) that binds anthocyanins to facilitate their transport from cytosol to vacuole in the diploid strawberry Fragaria vesca . The parent of rap was the F. vesca variety ‘Yellow Wonder’ that develops white fruit due to a natural mutation in the FveMYB10 gene. Here, we investigated the application potential of RAP in modulating fruit colours by overexpression of RAP in F. vesca and knockout of RAP in the cultivated strawberry Fragaria × ananassa . Unexpectedly, the RAP overexpression in Yellow Wonder background caused formation of red fruit. In addition, the red coloration occurs precociously at floral stage 10 and continues in the receptacle during early fruit development. Transcriptome analysis revealed that the anthocyanin biosynthesis genes were not up-regulated in RAP-ox; rap myb10 flowers at anthesis and largely inhibited at the turning stage in fruit, suggesting a coloration mechanism independent of FveMYB10 . Moreover, we used CRISPR/Cas9 to knockout RAP in cultivated strawberry which is octoploid. Six copies of RAP were simultaneously knocked out in the T0 generation leading to the green stem and white-fruited phenotype. Several T1 progeny have segregated away the CRISPR/Cas9 transgene but maintain the green stem trait. Our results indicate that enhancing the anthocyanin transport could redirect the metabolic flux from proanthocyanidin to anthocyanin production at early developmental stages of fruit and that RAP is one promising candidate gene in fruit colour breeding of strawberry.
研究领域:
植物生物学
·
遗传学
·
分子生物学
·
农学
✓ 已验证
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Lingrong Wen,Mingyang He,Chunxiao Yin,Yueming Jiang,Donghui Luo,Bao Yang
Sour orange ( Citrus aurantium ) is a popular fruit with health benefits. Phenolics are the dominant bioactive compounds in this fruit. However, the full phenolics composition remains unclear. In this work, UHPLC-MS/MS was used to qualify and quantify the phenolics in sour orange fruits of cv. Morocco (MSO) and United (USO). Fifty-eight phenolics, including 26 flavonoid glycosides , 22 polymethoxylated flavonoids, 8 coumarins and 2 phenolic acids , were identified. Four limonoids were characterized. Flavonoid glycosides were the leading phenolics in MSO, while polymethoxylated flavonoids (including nobiletin, tangeritin and sinensetin) were dominant in USO. The phenolic extracts showed good antioxidant activities . USO extract had a significantly ( p < 0.05) stronger neuroprotective activity than MSO extract. Auraptene, nobiletin and tangeritin were highly responsible for the neuroprotective activity. The results were helpful for the development of functional foods with sour orange.
研究领域:
食品科学
·
药理学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yangjing Li,Wei Tian,Tao Chen,Yanfei Li,Runan Zhao,Yuxin Li,Xuesong Chi,Di Wu,Yinan Du,Jiangning Hu
Mitigating food spoilage from microbial infections remains a critical challenge in food preservation. Although Zn 2+ ions are used as chemical bactericides, their use alone often requires high doses. A novel nanomaterial, Zn@MNPs, combining photothermal properties with the controlled release of Zn 2+ , was synthesized through a coordination of Zn 2+ with melanin nanoparticles (MNPs) derived from cuttlefish ink. Zn@MNPs were capable of attaching onto the bacterial surfaces, enabling high-efficiency release of Zn 2+ under mildly acidic conditions typically associated with bacterial infections. This leads to sustained antibacterial activity, causing bacterial membrane rupture and leakage of intracellular components. Incorporating Zn@MNPs into polyvinyl alcohol (PVA)-based films improved their ability to block UV light and reduce oxygen and water vapor permeability. These films effectively reduced dehydration, preserved nutritional content, and extended fruit shelf life. This study highlights the potential of Zn@MNPs-based PVA films as biodegradable, bioactive packaging materials for enhancing food preservation and safety.
研究领域:
食品科学
·
材料科学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2022
·
IF 6.751
Jialiang Liu,Wanli Zhang,Meijiao Hu,Yonggui Pan,Yueming Jiang,Zhengke Zhang,Guoxiang Jiang
Previous studies indicate that melatonin , as an important bioactive molecule, appreciably improves cold tolerance in postharvest litchi fruit. However, whether other signaling molecules are involved in melatonin-induced cold tolerance in litchi fruit has not been clarified. In this study, the effect of the interplay between melatonin and nitric oxide (NO) on chilling stress tolerance in relation to redox homeostasis in litchi fruit was investigated. In the first experiment, exogenous applications of melatonin and sodium nitroprusside (SNP) showed similar efficacy in decelerating the development of chilling injury (CI), as indicated by lower CI index and higher retention of pericarp redness in litchi fruit during refrigeration, whereas the opposite effect was found with NO scavenger 2-(4-carboxyphenyl)− 4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO). The ameliorating effect of melatonin on CI was evidently impaired under cotreatment with melatonin and cPTIO (melatonin + cPTIO). In the second experiment, the biochemical response of litchi fruit to melatonin and melatonin + cPTIO during cold storage was analyzed. The results exhibited that melatonin triggered NO accumulation by activating nitrate reductase (NR) and nitric oxide synthase (NOS) in refrigerated litchi fruit. Melatonin-induced NO accumulation was reduced under treatment with melatonin + cPTIO, but this combined treatment did not affect the NR and NOS activities when compared to melatonin alone. Reactive oxygen species (ROS) in melatonin-treated fruit were markedly quenched by activating antioxidant enzymes and increasing antioxidant metabolite contents, thus alleviating membrane lipid peroxidation and maintaining membrane integrity. Melatonin also led to increased expression of oxidized protein repair-related genes, including LcMsrA1, LcMsrA2 , LcMsrB1 , LcMsB2 , LcTrx2 and LcNTR1. Moreover, an enhanced level of protein S -nitrosylation was observed in fruit upon melatonin treatment. However, the above physiological effect associated with improved fruit cold tolerance afforded by melatonin was partially counteracted by cPTIO. The results suggest that endogenous NO could mediate melatonin-induced cold tolerance in litchi fruit via regulation of redox status.
研究领域:
植物生理学
·
采后科学
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生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2022
·Vol. 15
·期 10
·2725-2733
·
IF 3.498
Sun Luping,Liu Hongyan,Chi Bingqing,Bian Yifei,Zhou Honglei,Tian Zhenhua
Quince ( Cydonia oblonga Mill.) is the one and only specie of the genus Cydonia in the Rosacea family. Quince contains bioactive constituents such as alkaloids, saccharides, flavonoids, and volatile oils. Nevertheless, seldom researches have concentrated on quince essential oil (QEO). The chemical composition, cellular activity, antioxidant activity, and α-glucose inhibition activity of the plant were studied. QEO was isolated using hydrodistillation technique, and the yield was 1.3 mL/kg. A total of 52 chemical constituents in QEO were determined by gas chromatography-mass spectrometry (GC–MS). The main components (> 3.0%) found in the QEO were methyl (Z)-octadec-6-enoate (16.2%), methyl linoleate (10.0%), cedrenol (8.8%), methyl hexadecanoate (7.2%), methyl palmitoleate (7.2%), dibutyl phthalate (5.4%), hexadecanal (4.7%), 12,15-octadecatrienoicacid, ethylester, (Z,Z,Z)-9 (4.2%), α-eudesmol (3.5%), terpinen-4-ol (3.3%), and palmitic acid (3.2%). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. Then, the α-glucose inhibitory activity of the essential oil was analyzed. We investigated the effects of this plant on NO production and cell viability in H 2 O 2 –induced oxidative stress HUVCEs. The results showed that the QEO had significant antioxidant activity and α-glucose inhibitory effect. Furthermore, the results revealed that QEO could protect HUVECs from oxidative stress and effectively inhibited NO production in that condition. To summarize, the results of this study demonstrated the excellent cellular and antioxidant activity of QEO, and it has potential biological significance in the treatment of cardiovascular diseases.
研究领域:
植物化学
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生物活性
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药理学
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细胞生物学
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2016
·
IF 2.835
Xiao-Qin Wang,Hui-Bo Qin,Qing-Lan Ma,Zhen-Feng Sun,Ke-Le Yan,Zhi-Yu Song,Kai Guo,Da-Meng Liu,Guang-Jin Chen,Chang-Yu Sun
A terrestrial plant fruit extract was adopted to prevent hydrate accumulation. Four groups with different polarities, water-soluble, n-butanol-soluble, ethyl acetate-soluble, and petroleum ether-soluble portions, were obtained by prefractionation of the plant-extract hydrate antiagglomerant (AA). The hydrate antiagglomeration effect was tested in a high pressure transparent sapphire cell, and the active components were found mainly in the ethyl acetate-soluble portion that accounts for approximately 4.0% of the whole plant extract. Through further separation and purification, the five kinds of components obtained proved experimentally to have the effect of preventing hydrate agglomeration. Their molecules and structural formulas were inferred by the high resolution mass spectrum and nuclear magnetic resonance analysis. Compared with the spectrum library, the components were determined to be eriodictyol, apigenin, naringenin, luteolin, and 5-(4-hydroxy-6,7-dimethoxy-3-methylchroman-2-yl) benzene-1,2,3-triol. The tests on the extracted components and the commercial products verified their effect of preventing hydrate agglomeration, where apigenin and luteolin performed better than others.
研究领域:
化学工程
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植物化学
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能源科学
✓ 已验证
产品关联
DOI
高影响因子
ENERGY & FUELS
·
2017
·
IF 3.091
Umar Adli Amran,Sarani Zakaria,Chin Hua Chia,Zhen Fang,Mohamad Zulfahdli Masli
Optimization of microwave-assisted liquefaction of oil palm empty fruit bunch fiber (EFB) and cellulose (EFBC) in ethylene glycol (EG) was carried out to produce polyols. The liquefaction residues and hydroxyl numbers of the resultant polyols from respective sources were studied and compared. EFB produced a minimum residue of 3.22% at the optimal parameters of 160 °C and 15 min. Meanwhile, optimum liquefaction of EFBC produced 1.03% residue at 175 °C and 40 min. The maximum hydroxyl numbers of both EFB (749.22 mg KOH/g) and EFBC (639.91 mg KOH/g) polyols were obtained at optimum conditions. FTIR analysis revealed the degradation mechanism of cellulose and lignin in EFB at different temperatures. Lignin was found to be liquefied easily at lower temperatures (130 and 145 °C). However, most of the cellulose began to be liquefied at the optimum temperature (160 °C) and severely degraded at higher temperatures (175 and 190 °C).
研究领域:
生物化学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2021
·
IF 6.953
Can Song,Feihong Huang,Linyu Liu,Quan Zhou,Dan Zhang,Qi Fang,Hui Lei,Hong Niu
Pectins have proven to be advantageous for human health as they regulate beneficial microbial communities and enhance immunity. The fruit of Clausena lansium (Lour.) Skeels (Wampee), also referred to as “treasure in fruit”, is rich in pectin polysaccharides . In this study, a homogalacturonan-type pectin (CCP2) with a molecular weight of 8.9 × 10 4 Da and degree of esterification of 42.86% was isolated from Wampee fruit. The gut microbiota regulation and phagocytosis-enhancing properties of CCP2 were examined in vivo and in vitro, respectively. Oral administration of CCP2 dramatically decreased the abundance of Bacteroidetes and increased the abundance of Firmicutes in intestinal bacteria in mice. The content of short-chain fatty acids in the feces also significantly improved. Moreover, CCP2 exhibited excellent phagocytosis-enhancing activities on RAW 264.7 macrophages. These results suggested that CCP2 could be a potential gut microbiota regulator and phagocytosis-enhancer, which could be used in food products to promote health through beneficial manipulation of gut microbiota.
研究领域:
食品科学
·
微生物学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2020
·
IF 3.004
Xinya Liu,Mengyuan Chen,Zilin Meng,Heng Qian,Sanbing Zhang,Runhua Lu,Haixiang Gao,Wenfeng Zhou
In this study, low-density deep eutectic solvent combined with dispersive liquid-liquid microextraction was applied to the extraction of five benzoylurea insecticides (BUs, including diflubenzuron, triflumuron, hexaflumuron, flufenoxuron, and chlorfluazuron) from beverages. Then the extracted and concentrated samples were analyzed and detected using the high-performance liquid chromatography combined with an ultraviolet detector. The DESs were synthesized by [P14,6,6,6]Cl as hydrogen bond acceptor and tetradecyl alcohol as hydrogen bond donor, and then characterized by Fourier transform infrared spectroscopy. In the experiment, the key factors affecting the extraction efficiency were screened by Plackett-Burman design and optimized with the central composite design. The extraction recovery rates were 85.91–95.12%. The limits of detection and correlation coefficients of the method were 0.30–0.60 μg L −1 and 0.9992–0.9997. Finally, the method was applied to determine the BUs in four beverage samples, and satisfactory recoveries, within the range of 76.87–101.19% were achieved. The present method has the potential to be applied to the detection of BUs in aqueous samples.
研究领域:
分析化学
·
食品科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Yating Lu,Dong Li,Li Li,Tarun Belwal,Yanqun Xu,Xingyu Lin,Zhenhua Duan,Zisheng Luo
Degreening is widely used in citrus fruit to extend the market season for economic gains and increase the consumer acceptance. Elevated CO 2 was exogenously applied to Satsuma mandarins ( Citrus unshiu Marc. ) for degreening and its effect on pigment metabolism was investigated. The results revealed 15% CO 2 treatment accelerated the citrus fruit peel color change along with the chlorophyll degradation, β-cryptoxanthin and flavonoids accumulation. The expression of CitSGR , CitNYC , CitChlase , CitPPH , CitPAO and CitRCCR genes involved in chlorophyll metabolism and a set of genes involved in producing β, β-xanthophylls were up-regulated by elevated CO 2 . For flavonoid metabolism, the up-regulated expressions of CitPAL , CitCHS and CitCHI partly explained the increased total flavonoids content. These results showed that 15% CO 2 treatment improved the visual appearance of citrus fruits due to its impact on pigment metabolism and also maintained their nutritional value, thus could be employed as a potential commercial technique for citrus degreening.
研究领域:
植物学
·
园艺学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2019
·
IF 3.927
Lu Li,Xu Tang,Qiuli Ouyang,Nengguo Tao
Sodium dehydroacetate (SD) and sodium silicate (Na 2 SiO 3 ) are food preservatives with antifungal activities against many pathogens. In this paper, the antifungal activity of the combination treatment of SD and Na 2 SiO 3 against Geotrichum citri-aurantii , the postharvest pathogen of sour rot in citrus fruit, was tested. SD and Na 2 SiO 3 combination (1:4, w/w) showed a synergistic inhibitory effect compared to single compound on the mycelial growth of G. citri-aurantii , with the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) being 1.8 and 3.6 g L −1 , respectively. The spores’ germination and the plasma membrane integrity of the pathogen could also be visibly influenced by the 1× MFC SD + Na 2 SiO 3 treatment. The application of various SD + Na 2 SiO 3 concentrations (1×, 5×, and 10× MFC) to citrus fruit inoculated with G. citri-aurantii significantly ( P < 0.05) decreased the incidence of sour rot during the entire storage period. After 8 d of storage, the disease incidences in SD + Na 2 SiO 3 (10×, 5× or 1× MFC)-treated fruit were only 20%, 30%, 65%, respectively, in contrast to 100% of the control fruit. Meanwhile, SD + Na 2 SiO 3 treatments maintained the fruit quality of citrus fruit. Results indicate that the combination of SD and Na 2 SiO 3 is a promising approach in controlling the postharvest decay of citrus fruit.
研究领域:
食品科学
·
植物病理学
·
采后技术
✓ 已验证
产品关联
DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2022
·
IF 4.342
Xiaoya Tao,Qiong Wu,Suqing Huang,Beiwei Zhu,Feng Chen,Bin Liu,Luyun Cai,Linchun Mao,Zisheng Luo,Li Li,Tiejin Ying
Primary metabolites play crucial roles during fruit development and ripening. Phytohormone abscisic acid (ABA) has been reported to regulate postharvest tomato fruit ripening. However, knowledge about ABA in regulating primary metabolism in tomato fruit is obscure. In the present research, cherry tomato fruit were infiltrated with 1.0 mM ABA or deionized water under vacuum, and kept in darkness for 15 d. The changes of the relative contents of sugars, organic acids, and amino acids, as well as the expression levels of key genes involved in sugar and organic acid metabolisms, were analyzed. Results showed exogenous ABA accelerated tomato fruit ripening, accompanying with the enhanced changes of fruit color and firmness, the higher levels of respiration rate and ethylene production. Meanwhile, exogenous ABA enhanced the relative levels of some primary metabolites, such as glucose, lyxose and fructose; citric acid, citramalic acid, ascorbic acid and gluconic acid; also serine, pyroglutamic acid, GABA, glutamic acid, aspartic acid, asparagine and glutamine (1.69-3.25 folds). Furthermore, exogenous ABA stimulated the expression levels of most key genes (such as SPSs, SPP2, TIV1, Lins, SUSs, PPCs, MDH and CS ) (log 2 fold change ranged from 1.06 to 3.64). Results indicate ABA is systematically involved in the regulation of primary metabolism, which may provide valuable information for exploring technical means to improve tomato taste and flavor.
研究领域:
植物生理学
·
园艺学
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）