MATERIALS & DESIGN
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2025
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IF 7.9
Jingxia Chen,Jing Xu,Huixin Lv,Jiaxin Luo,Zhen Ci,Yangfan Pei,Yihan Wang,Xiuyu Liu,Sicong Ren,Sheng Chen,Siyu Chen,Ting Zhu,Jingjie Zhai,Yanmin Zhou
Jawbone defects pose significant challenges for oral implantation and restoration. Guided bone regeneration is the most common strategy for bone augmentation. However, inadequate efficiency of osteogenesis remains a persistent issue. Periosteum serves as a potential source of osteogenesis, but insufficient mesenchymal stem cells (MSCs) in periosteum often exist due to the lack of initial bone support. Inadequate blood supply near the submembranous osteogenic region also impedes the osteogenesis process. This paper proposes a dual cross-linked natural protein-derived hydrogel, GelMA-fibrinogen/NELL-1(GFN), which optimizes both physical and biological factors to stabilize the elevated maxillary sinus membrane (MSM) (an anatomical structure involved in osteogenesis in maxillary posterior dental region, resembling periosteum) and support the osteogenic space. The results showed stem cells derived from the membrane could migrate to GFN and exhibit robust proliferation capability. Under osteogenic induction, the adequately expanded MSM-derived MSCs enhanced osteogenic differentiation under the synergetic guidance of fibrinogen and Nell-1 through TGF-β/HIF-1α/β-catenin signaling pathway. In vivo experiments showed that GFN could promote bone formation with double-layered cortical bone structures in maxillary sinus region, which provides a new direction for construction of bone grafting materials.
研究领域:
生物材料
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组织工程
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再生医学
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口腔种植学
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干细胞生物学
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Wenlong Yuan,Yitong Sun,Bo Du,Ruiping Duan,Yanping Shi,Lingrong Liu,Xuemin Li
Gelatin methacryloyl (GelMA) hydrogel based tissue-adhesives have attracted extensive attraction due to its high biocompatibility, however, it suffers from insufficient mechanical properties, low adhesion strength due to the single network. In this study, a novel double-network hydrogel (DN-GelMA-Cur) consisting of GelMA and curdlan (Cur) was developed, where the network composed by triple helix fibers of Cur induced by heating and annealing serves as the hard network, while photo-crosslinked GelMA serves as the flexible network. A series of characterizations of the hydrogels, including hydrogel-forming ability, swelling behavior, morphology, rheological and mechanical properties, tissue adhesion, and biocompatibility were performed. It was found that precursor solution, especially with lower ratios of Cur (Cur: GelMA, 2:8 and 1:9), showed better hydrogel forming ability. Those hydrogels with lower ratios of Cur had better structural maintaining abilities, higher elastic modulus compared with pure-GelMA hydrogel. Particularly, addition of lower ratios of Cur could significantly enhance tissue-adhesion strength of GelMA hydrogel. DN-GelMA-Cur hydrogels showed good cytocompatibilities, especially hydrogels with lower ratios of Cur could promote proliferation of rat peritoneal fibroblasts significantly. Therefore, DN-GelMA-Cur hydrogels with lower ratio of Cur have potential application as novel GelMA hydrogel based tissue-adhesives with enhanced mechanical and bio-adhesive properties.
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PubMed
高影响因子
Materials
·
2026
·Vol. 19
·期 10
·2086
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IF 3.7
Dazhong Jin,Miaomiao He,Guangfu Yin
Surgical intervention is a primary treatment for osteosarcoma, often resulting in a tumorous bone defect with an irregular shape. Postoperative management is essential to minimize tumor recurrence risks and promote bone regeneration. To address these issues, we developed a multifunctional injectable, rapidly photo-curable hydrogel composed of gelatin methacryloyl/carboxymethyl chitosan/hyaluronic acid (GelMA/CMCS/HA), modified with vanadium-doped mesoporous bioactive glass (VMBG). The exceptional injectability enables seamless adaptation to irregular bone defects, offering a significant advantage over preformed implants, while the rapid photocurability of the hydrogel ensures stable fixation within minutes, thereby reducing potential risks during surgery. Furthermore, this platform exhibits dual therapeutic efficacy, characterized by antitumor activity and osteogenic induction. In vitro assessments demonstrated that V(V)/V(IV) valence cycling-driven ROS generation mediated its potent antitumor efficacy. Additionally, concurrent enhancement of alkaline phosphatase activity and osteogenic marker expression validated its osteogenic potential. The CMCS incorporation promoted healing at the defect site, while the HA addition created binding sites for cell adhesion and growth, thereby improving scaffold bioactivity. Collectively, this study presents the development and validation of a multifunctional GelMA/CMCS/HA hydrogel, highlighting its dual capability for bone regeneration and tumor suppression within tumor-associated bone microenvironments.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2023
·
IF 15.1
Zhenming Yang,Shuangshuang Fan,Wei Ma,Zhiguang Li,Xuehong Ren
Complex bacterial infection and inevitable movement are the two most concerning issues in joint wound healing. In this work, N -halamine structure was introduced into polydopamine nanoparticles (PDNPs) and encapsulated in a gelatin methacryloyl (GelMA)/silk fibroin (SF) matrix to endow the patches with efficient and broad-spectrum antibacterial activity. Zone of inhibition and in vivo antibacterial tests confirm that the addition of N -halamine containing organic nanoparticles as antibacterial components is a convenient and feasible strategy in the development of wound healing antibacterial materials. Here, dopamine methacrylamide (DMA) was synthesized and grafted onto the gelatin methacryloyl in patches with UV irradiation to get a reasonable adhesion. The liver prick injury model showed that the flexible adhesive GelMA/SF-based patch could be fixed on the wound area with rapid hemostatic effect. Furthermore, the resultant patches possess good flexibility, breathability and excellent biocompatibility. The histology analysis indicated the designed patches demonstrated less inflammatory response, significantly enhanced vascularization and the regeneration of dermis and epidermis. In summary, these N -halamine hemostatic GelMA/SF patches hold a promise for diverse practical acute trauma treatments.
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高影响因子
Biomaterials Advances
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2022
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IF 0
Yingchun Li,Yannan Liu,Bo Peng,Xinyue Li,Tianshu Fang,Shuai Liu,Jiachen Liu,Bo Li,Fei Li
Deep skin wound needs a long wound healing process, in which external force on skin around wound can result in a sharp pain, wound re-damage and interstitial fluid flowing out, increasing the risk of deterioration and even amputation. While the conventional wound dressings cannot provide timely feedback of abnormal wound status and lose best time for wound treatment, real-time monitoring wound status is thus urgently needed for wound management. In this work, a breathable and stretchable electronic skin ( i.e. , e-skin) named CNTs/graphene/GelMA mat has been developed through electrospinning, ice-templating and in-situ loading method for evaluating wound status. The obtained porosity, swelling ratio and vapor transmission rate of the CNTs/graphene/GelMA mat are 55 %, 180 % and 3378.2 h −1 day −1 , respectively. And owing to the good porous, nanofibrous architecture and excellent breathability of the mat, L929 cells grow and well spread on the CNTs/graphene/GelMA mat. In addition, the gauge factors of the prepared conductive CNTs/graphene/GelMA mat as a strain sensor are 15.4 and 72.9 in the strain ranges of 0–70 % and 70–85 %, respectively, matching the mechanical performance of human skin. The sensitivity coefficient of the mat for moisture sensing is 12.05, indicating its high efficiency for monitoring and warning interstitial fluid outflow from wound. Furthermore, the integration of CNTs/graphene/GelMA mat with a portable device is feasible to monitor strain and moisture on a rat model with abdominal wound. The healing process of the wounds treated with CNTs/graphene/GelMA mat is similar to that of GelMA mat, indicating that the dosage of CNTs and graphene in the CNTs/graphene/GelMA mat has negligible effect on the mat histocompatibility. The CNTs/graphene/GelMA mat demonstrates the application potential in wound management, home medical diagnosis and human-machine interactions.
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PubMed
EUROPEAN POLYMER JOURNAL
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2022
·
IF 5.546
Ying Mao,Yujia Zeng,Yunxiao Meng,Yan Li,Lu Wang
Postoperative adhesions are very common complications after various abdominal surgeries. Although numerous strategies have been applied to reduce or prevent adhesion formation, the clinical efficacy is still far from satisfactory. In this study, a multilayer electrospun nanomaterial (GelMA-PLA/PGA/Lec), composed of lecithin-doped polylactide/polyglycolide nanofiber membrane (PLA/PGA/Lec) and gelatin methacrylate (GelMA) nanofiber hydrogel, was developed to act as a physical barrier for the first time. The resulting barrier exhibited unique micro/nanopore architecture, excellent permeability, and proper water content. Besides structural stability, the PLA/PGA/Lec layer endowed the barrier with good mechanical properties and anti-fibroblast adhesion. Meanwhile, the GelMA layer imparted an acid-neutralizing effect for alleviating acidic environment during degradation and a lubrication capacity for reduced interfacial friction as well as inhibited excessive fibroblast proliferation. These results indicated that the GelMA-PLA/PGA/Lec barrier with efficient lubrication and anti-fibroblast capability has potential application in minimizing postoperative adhesions.
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高影响因子
Biomaterials Advances
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2025
·
IF 6
Mingzhu Zhai,Hongxi Tan,Aohong Xu,Benqing Wu,Fang Xie,Yuanzhi Lu,Yi Zheng
Diabetic foot ulcers (DFU), a severe complication of diabetes mellitus, present a global healthcare challenge due to high risks of limb amputation and mortality. This study developed a multifunctional hydrogel dressing, Gelatin Methacryloyl (GelMA) hydrogel loaded with interleukin-4 (IL-4)-engineered exosomes (Exos), designed to synergistically modulate immune responses and enhance angiogenesis for complete diabetic wound repair. The programmed death-ligand 1 (PD-L1)-enriched Exos were engineered via IL-4 overexpression in NIH3T3 fibroblasts. The GelMA hydrogel loaded NIH3T3 fibroblast-derived Exos with IL-4 overexpression (GelMA/Exos IL-4 hydrogel) exhibited favorable physicochemical characteristics, including a three-dimensional porous microstructure, injectability, tissue adhesion, self-healing properties, and sustained moisture retention. In vitro evaluation demonstrated biocompatibility, sustained exosome release, and enhanced viability, migration, and tube formation of human umbilical vein endothelial cells (HUVECs). In a diabetic wound model, the hydrogel significantly accelerated wound closure, promoted re-epithelialization and angiogenesis, and skewed macrophages toward anti-inflammatory M2 polarization while suppressing T-cell proliferation. These findings highlight the dual immunomodulatory and pro-angiogenic efficacy of GelMA/Exos IL-4 hydrogel, offering a promising therapeutic strategy for chronic wound management.
研究领域:
生物材料学
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再生医学
·
免疫学
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PubMed
高影响因子
MATERIALS & DESIGN
·
2021
·
IF 7.991
Jianing Yan,Xixia Liu,Jingyi Liu,Xinjie Zhang,Qiang Zheng,Junjie Nan,Mengjia Lin,Haoqi Pan,Yifan Wang,Xiujun Cai,Jun Yin
Bile duct injury is a common complication of hepatobiliary surgeries in clinical practice with poorly satisfactory treatment outcomes. Implanting a tissue-engineered tubular scaffold for supporting and replacing defected native bile duct is an important treatment option for bile duct injury. Here a dual-layer tubular scaffold with poly (lactic-co-glycolic acid) (PLGA) inner layer and gelatin methacrylate (GelMA)/poly (ethylene glycol) diacrylate (PEGDA) outer layer was fabricated using a two-stage molding method for bile duct regeneration. Further, induced mesenchymal stem cells (MSC)-derived cholangiocyte-like cells were embedded in the outer layer during fabrication. The inner layer of PLGA offered adequate mechanical strength for the scaffold, and the outer layer of GelMA/PEGDA provided excellent biocompatibility and conditions for the MSCs and their induced differentiation. Moreover, cholic acid and its derivates are believed to induce MSC-cholangiocyte differentiation in two-dimensional culture and could affect the MSCs transforming to cholangiocyte-like cells in the hydrogel. Induced-MSC-laden GelMA/PEGDA-PLGA dual-layer tubular scaffold and PLGA single-layer scaffold were transplanted into animal models for assessing the repair effect on bile duct injury. After 12 wk, induced-MSC-laden dual-layer tubular scaffold promoted bile duct repairing and enhanced more intact biliary epithelium regenerating compared to PLGA single-layer scaffold. Therefore, the induced-MSC-laden dual-layer tubular scaffold with superior biocompatibility and distinct function of inducing differentiation exerted a satisfactory effect on bile duct regeneration and could provide an ideal option for the clinical treatment of bile duct injury in the future.
研究领域:
组织工程
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再生医学
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生物材料
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细胞生物学
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DOI
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
·
2021
·
IF 7.328
Zongxi Wu,Shang Xie,Yifan Kang,Xiaofeng Shan,Qing Li,Zhigang Cai
Peripheral nerve injury can cause various degrees of damage to the morphological structure and physiological function of the peripheral nerve. At present, compared with “gold standard” autologous nerve transplantation, tissue engineering has certain potential for regeneration and growth; however, achieving oriented guidance is still a challenge. In this study, we used 3D bioprinting to construct a nerve scaffold of RSC96 cells wrapped in sodium alginate/gelatin methacrylate (GelMA)/bacterial nanocellulose (BNC) hydrogel. The 5% sodium alginate+5% GelMA+0.3% BNC group had the thinnest lines among all groups after printing, indicating that the inherent shape of the scaffold could be maintained after adding BNC. Physical and chemical property testing (Fourier transform infrared, rheometer, conductivity, and compression modulus) showed that the 5% alginate+5% GelMA+0.3% BNC group had better mechanical and rheological properties. Live/dead cell staining showed that no mass cell death was observed on days 1, 3, 5, and 7 after printing. In the 5% alginate+5% GelMA group, the cells grew and formed linear connections in the scaffold. This phenomenon was more obvious in the 5% alginate+5% GelMA+0.3% BNC group. In the 5% alginate+5% GelMA+0.3% BNC group, S-100β immunofluorescence staining and cytoskeleton staining showed oriented growth. Polymerase chain reaction (PCR) array results showed that mRNA levels of related neurofactors ASCL1, POU3F3, NEUROG1, DLL1, NOTCH1 and ERBB2 in the 5%GelMA+0.3%BNC group were higher than those of other groups. Four weeks after implantation in nude mice, RSC96 cells grew and proliferated well, blood vessels grew, and S-100β immunofluorescence was positive. These results indicate that a 3D-bioprinted sodium alginate/GelMA/BNC composite scaffold can improve cell-oriented growth, adhesion and the expression of related factors. This 3D-bioprinted composite scaffold has good biocompatibility and is expected to become a new type of scaffold material in the field of neural tissue engineering.
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PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Yufeng Ai,Jingting Wu,Wenfeng Li,Fancheng Xu,Jianxin Wu,Li Li,Kaiqiang Yang,Chenjiang He,Pengyan Zhu,Xueli Leng,Fang Dai,Li Song
In the aging society, bone defects significantly threaten human health. Therefore, several biomaterials have been developed to promote bone regeneration. However, previous studies have predominantly focused on optimizing the osteoinductive capacity of materials while neglecting vascular regeneration and immune responses elicited by biomaterials. The repair of bone defects is a complex biological process that involves not only bone resorption and formation, but also inflammation, immunity, and vascularization. The present study successfully encapsulated deferoxamine (DFO) into poly (lactic-co-glycolic acid) (PLGA) to form nanospheres for the sustained release of DFO, thereby simulating a hypoxic microenvironment. DFO-PLGA/Bioactive glass (BG)/Bone marrow mesenchymal stem cells (BMSCs) @GelMA (DBB@GelMA) exhibited excellent biocompatibility and osteoinductive properties in vitro. Furthermore, DBB@GelMA continuously releases DFO to promote vascular regeneration, M2 polarization of macrophages, and osteogenic differentiation of BMSCs. The finding from the experiment on SD rat calvarial defect repair further validated its exceptional immunoregulatory, angiogenic, and osteoinductive capabilities. In conclusion, this composite hydrogel offers a novel strategy for regulating the hypoxic microenvironment and holds promise for bone-defect repair.
研究领域:
生物材料学
·
组织工程学
·
再生医学
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高影响因子
ACS Applied Materials & Interfaces
·
2026
·
IF 7.8
Meng Yuan,Rui Yin,Kun Liu,Tao Jiang,Xiaofan Yang,Xiang Xu,Pengjuan Nie,Shuoyuan Liu,Honglian Dai,Zhenbing Chen
Diabetic wound healing is a global biomedical challenge. Bacterial infection and microenvironmental modulation are major factors in the refractory healing of diabetic wounds. Conventional therapeutic approaches are often hindered by limitations, such as inadequate drug penetration, the rise of antimicrobial resistance, and restricted depth of drug delivery. Nanomedicine is emerging as a prospective drug delivery and anti-infective therapeutic approach in the field of wound management. Herein, all-trans retinoic acid (ATRA) was loaded into a zeolite imidazolate framework-91 salt+ (ZIF-91 salt+). A gelatin methacryloyl (GelMA) hydrogel microneedles (MNs) patch based on this multifunctional ZIFs (denoted as GelMA-ZIF-91 salt+@ATRA MNs patch) was developed, which makes transdermal drug delivery and combinatory treatment for diabetic wound healing feasible. Significantly, ATRA-containing ZIF-91 salt+ provides nanocomposites with anti-inflammatory activity through their modulation of macrophage polarization. Crucially, the prepared ZIF-91 salt+ showed efficient antibacterial activity against Staphylococcus aureus and Escherichia coli in vitro. Therapeutic effects of the GelMA-ZIF-91 salt+@ATRA MNs patch were verified in a diabetic mouse model with full-thickness cutaneous wounds. A unique MNs patch with antimicrobial, angiogenesis-inducing, and anti-inflammatory properties was designed in accordance with the physiological characteristics of wounds. Collectively, the designed MNs patch based on this multifunctional ZIF-91 salt+ provides new insights into diabetic wound therapy.
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PubMed
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2026
·
IF 5.2
Tianqi Wang,Shaoze Jing,Ke Ma,Fan Zhang,Bing Wang,Yu Lei,Jun Zhang
Osteoarthritis (OA) is a progressive degenerative joint disorder characterized by articular cartilage degradation, persistent oxidative stress, and chronic inflammation. Targeting oxidative damage within the joint microenvironment represents a promising avenue for disease modification, offering potential to preserve cartilage integrity, alleviate inflammatory cascades, and ultimately delay structural progression. Lipid nanoparticles were prepared by thin-film hydration and extrusion, then embedded into GelMA microspheres via a microfluidic droplet platform with UV crosslinking to form a sustained-release system. Biocompatibility, ROS-scavenging, anti-inflammatory, and chondroprotective effects were evaluated in vitro and in vivo, while transcriptome sequencing was applied to elucidate system-induced molecular alterations. In vitro , Api-LNP@GMS markedly attenuated ROS accumulation and inflammatory responses in IL-1β-stimulated chondrocytes, as demonstrated by ROS fluorescence imaging, immunofluorescence, Western blotting, and qRT-PCR analyses. In vivo , intra-articular administration in anterior cruciate ligament transection (ACLT) rats effectively alleviated joint inflammation while preserving cartilage integrity and functional capacity. Moreover, transcriptome-wide RNA sequencing, integrated with pathway enrichment analysis and validated by molecular assays, revealed robust activation of the Nrf2–HO-1 antioxidant signaling axis. Api-LNP@GMS is a multifunctional antioxidant biomaterial platform that integrates a natural therapeutic with a sustained-release delivery system, offering a safe and effective strategy to reprogram the osteoarthritic microenvironment and mitigate disease progression. Download: Download high-res image (281KB) Download: Download full-size image
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