查找使用阿拉丁产品的科研文献

Speciation analysis of six arsenic species in marketed shellfish: Extraction optimization and health risk assessment

Yuyu Jia,Lin Wang,Li Ma,Zhaoguang Yang

A comparative study between microwave assisted and sonication methods was carried out to extract arsenic (As) species in shellfish samples using different extractants. Six As species including arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid (MMA), dimethylarsinic acid (DMA), arsenobetaine (AsB) and arsenocholine (AsC) were simultaneously separated and determined by the HPLC–ICP-MS method. The microwave assisted method exhibited higher efficiency than sonication, especially using diluted HNO 3 as extractant. By compromising extraction efficiency, pretreatment time and stability of As species, the microwave assisted method using 1% HNO 3 at 100   °C for 1.5   h was selected to extract As from real samples. The proposed method has been applied to extract and determine As species in shellfish samples. The result of correlation analysis indicated that the proportion of AsB in the shellfish samples was decreased with total As concentration increasing due to the biotransformation threshold from inorganic As to AsB.

Determination of trace elements in salt lake brines using inductively coupled plasma optical emission spectrometry after magnesium hydroxide precipitation

Liping Wang,Haijun Li,Yaping Dong,Jiaoyu Peng,Haitao Feng,Wu Li

A magnesium hydroxide coprecipitation method for the determination of trace elements in high-salt samples from salt lake brines by inductively coupled plasma optical emission spectrometry (ICP-OES) was developed. Some effects on the coprecipitation and analysis, such as the pH value and Na and Mg amounts in primary brines, have been thoroughly investigated and optimized. After adjusting the pH to 8.70, associated with sodium hydroxide, the complicated matrix was removed from high salt brines, and thus trace amounts of Al, Co, Cu, Fe, Mn, Ni, V, and Zn were simultaneously coprecipitated and precisely determined. Recoveries of all the determined trace elements in the samples of both synthesized and real salt lake brines were more than 90%. The detection limit in the range of 0.62–14.4 μg L−1 was obtained and the precision was less than 3% relative standard deviation. The optimized method has been successfully applied for the determination of trace elements in the intercrystal brine mined from Dachaidan salt lake located in the Qaidam Basin, China.

Mechanism-based cytotoxicity trend prediction of furan-containing pollutants present in a mixture

Wei Li,Zixia Hu,Chenyang Jia,Wei Guo,Weiwei Li,Ying Peng,Jiang Zheng

Human exposure to furan-containing pollutants (FCPs) has raised concerns due to their high risk of toxicity. A substantial number of approximately 8500 recorded compounds containing a furan ring exist which have been analytically or in biologically studied. A significant portion of these compounds is found in the everyday environments of individuals, particularly when ingested through food. Consequently, there is a need for a universal approach to rapidly predict the potential toxicity trends of FCPs. In this study, we developed a bromine labeling-based platform that combines LC-ICP-MS and LC-ESI-MS techniques to absolutely quantify FCP-induced protein adduction. The LC-ESI-MS approach facilitated the identification of FCP-derived protein adducts and optimized liquid chromatographic conditions for analyte separation. By employing a well-designed bromine-containing compound as a general internal standard, LC-ICP-MS-based technique enabled to absolutely assess bromine-labeled protein adduction. The protein adduction efficiencies of furan, 2-methylfuran, and 2,5-dimethylfuran were found to be 2.68, 2.90, and 0.37 molecules per 10,000 FCP molecules that primary hepatocytes received, respectively. Furthermore, we observed that 2-methylfuran exhibited the highest cytotoxicity, followed by furan and 2,5-dimethylfuran, which aligned with the order of their protein adduction. Thus, the protein adduction efficiency of FCPs could serve as a potential index for predicting their toxicity trends.

Speed and precision: unveiling an ICP-MS method for ultra-trace 239Pu quantification in food

Yinjie Zou,Hong Ren,Lei Zhou,Peng Wang,Yiyao Cao

A rapid, accurate, and sensitive inductively coupled plasma mass spectrometry (ICP-MS) method has been developed and validated for the determination of ultra-trace levels of Plutonium-239 ( 239 Pu) in food. After ashing, samples were digested with lefort aqua regia and treated with FeCl 3 to mask phosphorus (P) interference, thereby preventing it from inhibiting the adsorption of Pu by the resin column. Subsequently, a two-stage TK200 resin column was employed for separation and purification, followed by ICP-MS analysis using kinetic energy discrimination (KED) mode. By combining a chromatographic column with ICP-MS, this method achieved an overall decontamination factor (DF) of 3.53×10 12 for uranium hydride ions ( 238 UH + ) and demonstrated excellent linearity within the given concentration range, with a correlation coefficient ( r ) exceeding 0.999. The limit of detection (LOD) was 3.6 fg/g (ash), with an average recovery of over 74% and a relative standard deviation below 6.6%. Furthermore, the proposed method's applicability was evaluated by quantifying 239 Pu in food. The results indicate that this method is rapid, accurate, and sensitive, making it ideal for 239 Pu detection.

Accurate determination of carbonate-associated phosphate (CAP) by ICP-OES following cation-exchange resin separation

Zhiguang Xia,Mengting Zhang,Zihu Zhang,Xinyang Chen,Ying Gao,Jun Hu,Chunxia Yang,Thomas J. Algeo,Chao Li

An improved and robust method for determining carbonate-associated phosphate (CAP) is presented. • AG50W-X12 effectively purifies P from carbonate samples with high Mg Ca matrices. • The use of the P 177.495 nm emission line enables a low detection limit of <3 ppb. • CAP values for carbonate reference materials (GSR-13, GSR-23 and Jdo-1) agree well with published data and exhibit improved precision. • CAP values of five carbonate standards (GSR-26, GSR-27, GSR-28, GSR-29, and BCS-CRM 393) are reported for the first time. Carbonate-associated phosphate (CAP) serves as a direct proxy for reconstructing past oceanic phosphorus (P) concentrations and provides valuable insights into the co-evolution of marine P cycling, primary productivity, and atmospheric oxygen levels throughout Earth's history. However, current CAP analytical methods face challenges, including poor reproducibility, high detection limits, and a scarcity of geological reference material datasets for inter-laboratory comparison. In this study, we developed a method to purify P from carbonate samples with high Ca Mg matrices using AG50W-X12 cation-exchange resin, followed by quantitative determination by ICP-OES using the strongest P emission line at 177.495 nm, achieving a detection limit for CAP values of 0.002 mmol/mol. The methodological robustness with respect to variations in acid concentration, dissolution time, and the fraction of carbonate dissolved was evaluated through multiple independent experiments. The method was then applied to determine CAP values of eight carbonate geological reference materials (mmol/mol): CAP GSR-13  = 0.018 ± 0.002 (2σ, N  = 3, RSD = 4.2%); CAP GSR-23  = 0.061 ± 0.008 (2σ, N  = 8, RSD = 6.9%); CAP GSR-26  = 0.093 ± 0.014 (2σ, N  = 3, RSD = 7.3%); CAP GSR-27  = 0.025 ± 0.006 (2σ, N = 3, RSD = 12.3%); CAP GSR-28  = 0.047 ± 0.008 (2σ, N = 3, RSD = 8.9%); CAP GSR-29  = 0.051 ± 0.010 (2σ, N = 3, RSD = 9.6%); CAP BCS-CRM 393  = 0.027 ± 0.005 (2σ, N = 3, RSD = 9.4%); CAP JDo-1  = 0.147 ± 0.008 (2σ, N = 3, RSD = 2.6%). Compared with previously published CAP values for three standards: CAP GSR-13  = 0.022 ± 0.004 (2σ, RSD = 9.1%), CAP GSR-23  = 0.075 ± 0.015 (2σ, RSD = 9.7%) or 0.070 ± 0.014 (2σ, RSD = 10.0%), and CAP JDo-1  = 0.135 ± 0.012 (2σ, RSD = 4.6%), our results exhibit comparable accuracy but improved analytical precision. Long-term reproducibility of the GSR-23 standards was better than 0.008 mmol/mol (2σ) over a three-month measurement period. The CAP values for GSR-26, GSR-27, GSR-28, GSR-29, and BCS-CRM 393 are reported here for the first time. This high-precision analytical method enables the identification of subtle variations in P concentrations during carbonate formation and significantly facilitates future investigations into the evolution of marine phosphorus cycling. Download: Download high-res image (133KB) Download: Download full-size image

Development and application of a fluorescent immunochromatographic assay for metabolite G-DCA quantification in ICP

Jianyi Gao,Linxia Shuang,Xiaojin Yang,Ruirui Dong,Gaoying Wang,Rong Wang,Ting Zhang

Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disorder characterized by dysregulated bile acid metabolism, which can lead to severe adverse pregnancy outcomes. Glycine-conjugated deoxycholic acid (G-DCA) is a small-molecule metabolite that has been reported to be elevated in the serum of patients with ICP; however, its clinical application remains limited because conventional chromatographic methods for its quantification are time-consuming, costly, and not well suited for rapid clinical screening. To address this gap, we developed a time-resolved fluorescence immunochromatographic test strip (TRF-ICTS) for the quantitative detection of serum G-DCA. Based on a small-molecule competitive binding design, the assay requires only 10 μL of serum sample and enables rapid detection within 16 min. The TRF-ICTS demonstrated good analytical performance, with a linear range of 0.05–10.0 ng/mL and a detection limit of 0.074 ng/mL, and maintained stable performance over two weeks of storage at both 4 °C and 37 °C. The results showed good quantitative agreement with LC–MS/MS measurements. Evaluation of sensitivity, specificity, and precision demonstrated that all parameters were within acceptable ranges. Clinical validation using serum samples from ICP patients and healthy pregnant women showed that G-DCA levels were elevated across different trimesters and were more pronounced in patients with severe ICP and adverse pregnancy outcomes. Overall, the developed TRF-ICTS provides a rapid and sensitive approach for quantifying G-DCA and supports its use as a complementary biomarker for ICP diagnosis and disease stratification, with potential to provide complementary insights into bile acid changes following UDCA treatment.

The compound-independent calibration of alkyl organophosphorus flame retardants in rice using GC-ICP-MS

Chen Hongze,Wan Yuebing,Li Kangcong,Li Xiuqin,Li Xiaomin,Chu Hongtao,Zhang Qinghe

Organophosphorus flame retardants (OPFRs) are widespread environmental contaminants with bioaccumulative potential and associated human health risks. The lack of commercial reference standards for many OPFRs limits the accuracy of their quantification. In this study, a compound-independent calibration (CIC) method using gas chromatography-inductively coupled plasma mass spectrometry (GC-ICP-MS) was developed for the simultaneous quantification of seven alkyl OPFRs, with tripropyl phosphate (TnPP) as a single universal calibrant. Key parameters were optimized: a DB-5MS column (15 m × 0.25 mm × 0.10 μm) with pulsed splitless injection (70 psi, 280 °C), and ICP-MS settings including an RF power of 1600 W, sampling depth of 7.5 mm, dilution gas flow rate of 0.5 mL/min, and octopole voltage of − 5 V. The method exhibited excellent linearity ( R 2  = 0.9994 to 0.9999) over the range of 0.01–1.0 μg/mL. Limits of detection and quantification were 0.22 to 0.91 ng/mL and 0.75 to 3.02 ng/mL, respectively. For six of the seven OPFRs (excluding TEHP), the relative P response ratios compared to TnPP ranged from 0.91 to 1.01, confirming effective structure-independent calibration. The matrix effect in rice was moderate (enhancement factors 1.13–1.35). Quantitative results obtained by the CIC method agreed well with those from an external standard approach, with relative deviations within −8.54% to +7.78%. This work demonstrates that CIC-GC-ICP-MS using a single, readily available calibrant provides a reliable, practical solution for quantifying alkyl OPFRs in complex food matrices, effectively overcoming the challenge posed by the scarcity of compound-specific reference standards. Graphical The alternative text for this image may have been generated using AI.

High-precision molybdenum isotope analysis of low-Mo igneous rock samples by MC–ICP–MS

Jing-Jing Fan,Jie Li,Qiang Wang,Le Zhang,Jing Zhang,Xiang-Lan Zeng,Lin Ma,Zi-Long Wang

Recent advances in analytical techniques have achieved high-precision (2σ ≤ 0.06‰) determinations of Mo isotopic ratios in silicate rocks but few such data are as yet available, especially for low-Mo (<50 ppb) rocks such as leucogranites . Here we present two low-blank, double-spike, high-yield, ion-exchange chromatographic methods (single- and two-column) for precise determination of Mo isotopic ratios in low-Mo rock samples (10–500 ppb) using multicollector–inductively coupled plasma–mass spectrometry (MC–ICP–MS). The two-column technique involved AG 1-X8 anion-exchange (100–200 mesh) and BPHA chelatin resins, with the latter also used in the single-column procedure. Two USGS reference materials (basalt BIR-1a and diabase W-2a) were analyzed for quality-control purposes. Their δ 98/95 Mo values determined using the two methods are consistent within uncertainties, and with those determined by previously published methods. We provide the first Mo isotopic data for the reference materials of dunite DTS-2b (USGS), GPt-3 (NRCG) and peridotite WPR-1 (CCRMP). δ 98/95 Mo values for DTS-2b and WPR-1 by using two-column procedure are in good agreement with those obtained by single-column separation. Repeated analyses of these standards indicate a long-term external reproducibility of better than ±0.05‰. However, the δ 98/95 Mo values of GPt-3 show poor reproducibility due to either the sample heterogeneity or existing undigested minerals (e.g., spinel). The methods were applied to leucogranites with low Mo contents of 10–74 ppb from the Himalaya orogen and southern Lhasa terrane of Tibet . The isotopic analyses yielded δ 98/95 Mo values of −0.2‰ to 0.34‰ and −0.41‰ to −0.68‰ (against NIST SRM 3134), respectively, within the range of values for igneous crust. Their contrasting Mo isotopic compositions may indicate compositional diversity in their sources or differences in magma evolution .

Persistent Luminescence Nanoplatform for Autofluorescence-Free Tracking of Submicrometer Plastic Particles in Plant

Fang Hao,Zhu-Ying Yan,Zhenyu Wang,Xiu-Ping Yan

The uptake of plastic particles by plants and their transport through the food chain make great risks to biota and human health. Therefore, it is important to trace plastic particles in the plant. Traditional fluorescence imaging in plants usually suffers significant autofluorescence background. Here, we report a persistent luminescence nanoplatform for autofluorescence-free imaging and quantitation of submicrometer plastic particles in plant. The nanoplatform was fabricated by doping persistent luminescence nanoparticles (PLNPs) onto polystyrene (PS) nanoparticles. Cr3+-doped zinc gallate PLNP was employed as the dopant for autofluorescence-free imaging due to its persistent luminescence nature. In addition, the Ga element in PLNP was used as a proxy to quantify the PS in the plant by inductively coupled plasma mass spectrometry (ICP-MS). Thus, the developed nanoplatform allows not only dual-mode autofluorescence-free imaging (persistent luminescence and laser-ablation ICP-MS) but also ICP-MS quantitation for tracking PS in plant. Application of this nanoplatform in a typical plant model Arabidopsis thaliana revealed that PS mainly distributed in the root (>99.45%) and translocated very limited (<0.55%) to the shoot. The developed nanoplatform has great potential for quantitative tracing of submicrometer plastic particles to investigate the environmental process and impact of plastic particles.

Extraction and Quantification of Nanoparticulate Mercury in Natural Soils

Weiping Cai,Yujun Wang,Yu Feng,Peng Liu,Shuofei Dong,Bo Meng,Hua Gong,Fei Dang

Nanoparticulate mercury (Hg-NPs) are ubiquitous in nature. However, the lack of data on their concentration in soils impedes reliable risk assessments. This is due to the analytical difficulties resulting from low ambient Hg concentrations and background interferences of heterogeneous soil components. Here, coupled to single particle inductively coupled plasma-mass spectrometry (spICP-MS), a standardized protocol was developed for extraction and quantification of Hg-NPs in natural soils with a wide range of properties. High particle number-, particle mass-, and total mass-based recoveries were obtained for spiked HgS-NPs (74–120%). Indigenous Hg-NPs across soils were within 107–1011 NPs g–1, corresponding to 3–40% of total Hg on a mass basis. Metacinnabar was the primary Hg species in extracted samples from the Wanshan mercury mining site, as characterized by X-ray absorption spectroscopy and transmission electron microscopy. In agreement with the spICP-MS analysis, electron microscopy revealed comparable size distribution for nanoparticles larger than 27 nm. These indigenous Hg-NPs contributed to 5–65% of the measured methylmercury in soils. This work paves the way for experimental determinations of indigenous Hg-NPs in natural soils, which is critical to understand the biogeochemical cycling of mercury and thereby the methylation processes governing the public exposure to methylmercury.

Cadmium and cobalt ions enhanced-photochemical vapor generation for determination of trace rhenium by ICP-MS

Yafei Zhen,Hanjiao Chen,Meng Zhang,Jing Hu,Xiandeng Hou

A new two-stage purification method for high-precision indium isotope measurement by MC-ICP-MS

Bingyu Peng,Qiaohui Zhong,Gaobin Chu,Jie Li

Conventional indium (In) separation methods based on anion-exchange resins typically involve multiple evaporation and medium-transfer steps or require large volumes of concentrated acids, leading to prolonged processing times and potential contamination risks. Herein, we present an efficient two-stage column separation method for the separation and purification of In from complex geological matrices using two self-made trioctylamine (N235) and tri-n-octylphosphine oxide (TOPO) resins, enabling high-precision In isotopic analysis by multi-collector inductively coupled plasma mass spectrometry (MC-ICP-MS). In the first column, most matrix elements were efficiently removed in HBr medium, and the In fraction together with trace amounts of Mo and Sn were eluted using 0.2 M HCl, and were ready for a second column purification without any evaporation. Subsequently, the residual interference of Mo and Sn was quantitatively removed from the In fraction using the TOPO resin column. This method not only eliminates hazardous reagents such as hydrofluoric acid (HF) but also significantly reduces resin and acid consumption compared to previous methods, achieving high In recoveries (>95%) and low procedural blanks (<0.2 ng). For In isotopic analysis, a combination of standard-sample bracketing with an Sb external method was employed for instrumental mass bias correction. NIST SRM 3124a In standard solution, for the first time, was adopted as a reference with an external reproducibility of 0.07‰ (2SD, n = 233) over a year. The method was validated using nine diverse geological reference materials (RMs). The In isotopic compositions obtained for three internationally recognized RMs were in good agreement with previously reported literature values. For the six other RMs, In isotope data are reported here for the first time, and they exhibit excellent external reproducibility. Moreover, all purified samples showed negligible isobaric interferences and matrix effects during MC-ICP-MS analysis, confirming the method's suitability for challenging geological samples.