Cells
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2024
·Vol. 13
·期 3
·206
·
IF 5.1
Meng-Yuan Li,Ying Wang,Ying Wu,Xu-Yu Zhao,Zhen-Shan Yang,Bo Li,Si-Ting Chen,Yu-Ying He,Zeng-Ming Yang
(1) Background: Inflammatory responses are implicated in embryo implantation, decidualization, pregnancy maintenance and labor. Both embryo implantation and decidualization are essential to successful pregnancy in rodents and primates. S100A6 is involved in inflammation, tumor development, apoptosis and calcium homeostasis. S100A6 is strongly expressed in mouse decidua, but the underlying mechanisms of how S100A6 regulates implantation and decidualization are poorly defined. (2) Methods: Mouse endometrial stromal and epithelial cells are isolated from day 4 pseudopregnant mouse uteri. Both immunofluorescence and Western blotting are used to analyze the expression and localization of proteins. The molecular mechanism is verified in vitro by Western blotting and the quantitative polymerase chain reaction. (3) Results: From days 4 to 8 of pregnancy, S100A6 is specifically expressed in mouse subluminal stromal cells. Blastocyst-derived lactic acid induces AA secretion by activating the luminal epithelial p-cPLA2. The epithelial AA induces stromal S100A6 expression through the COX2/PGI2/PPAR δ pathway. Progesterone regulates S100A6 expression through the progesterone receptor (PR). S100A6/RAGE signaling can regulate decidualization via EGFR/ERK1/2 in vitro. (4) Conclusions: S100A6, as an inflammatory mediator, is important for mouse implantation and decidualization.Keywords:decidualization;implantation;S100A6;RAGE;inflammation
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Biosensors-Basel
·
2025
·Vol. 15
·期 1
·49
·
IF 5.6
Yijiong Xue,Chengqi Bao,Hui Liu,Fanghui Ma,Minghui Yang,Xiaoqing Li
A fluorescence probe for “switch-on” detection of alkaline phosphatase (ALP) was developed based on Au nanoclusters anchored MnO2nanosheets (Au NCs-MnO2NSs), which were synthesized using bovine serum albumin (BSA) as template through a simple one-pot approach. In the sensing system, MnO2NSs function as both energy acceptors and target identifiers, effectively quenches the fluorescence of Au NCs via fluorescence resonance energy transfer (FRET). The presence of ALP catalyzes the hydrolysis of L-ascorbic acid-2-phosphate (AAP) to ascorbic acid (AA), reducing MnO2NSs to Mn2+and facilitate the fluorescence recovery of Au NCs. The fluorescence assay offers the advantages of facile preparation, cost-effectiveness, good specificity, and high sensitivity. Moreover, the assay exhibits a broad linear range (0.005 U/mL to 8 U/mL) for ALP detection with a remarkable limit of detection of 0.0015 U/mL. Notably, this assay demonstrates promising applicability for detection ALP in human serum samples, thereby providing valuable potential for clinical applications.
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EUROPEAN JOURNAL OF PHARMACOLOGY
·
2024
·
IF 4.2
Ya-Ling Chen,Yi Wang,Qiu-Yu Fang,Tong Wang,Cong Chen,Tong-Yao Gao,Ming Wu,Wei-Ping Zhang,Yun-Bi Lu
Poly (ADP-ribose) polymerase-1 (PARP-1) activity significantly increases during cerebral ischemia/reperfusion. PARP-1 is an NAD + -consumption enzyme. PARP-1 hyperactivity causes intracellular NAD + deficiency and bioenergetic collapse, contributing to neuronal death. Besides, the powerful trigger of PARP-1 causes the catalyzation of poly (ADP-ribosyl)ation (PARylation), a posttranslational modification of proteins. Here, we found that PARP-1 was activated in the ischemic brain tissue during middle-cerebral-artery occlusion and reperfusion (MCAO/R) for 24 h, and PAR accumulated in the neurons in mice. Using immunoprecipitation, Western blotting, liquid chromatography-mass spectrometry, and 3D-modeling analysis, we revealed that the activation of PARP-1 caused PARylation of hexokinase-1 and lactate dehydrogenase-B, which, therefore, caused the inhibition of these enzyme activities and the resulting cell energy metabolism collapse. PARP-1 inhibition significantly reversed the activity of hexokinase and lactate dehydrogenase, decreased infarct volume, and improved neuronal deficiency. PARP-1 inhibitor combined with pyruvate further alleviated MCAO/R-induced ischemic brain injury in mice. As such, we conclude that PARP-1 inhibitor alleviates neuronal death partly by inhibiting the PARylation of metabolic-related enzymes and reversing metabolism reprogramming during cerebral ischemia/reperfusion injury in mice. PARP-1 inhibitor combined with pyruvate might be a promising therapeutic approach against brain ischemia/reperfusion injury.
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POLYMER COMPOSITES
·
2024
·
IF 4.8
Wei Jiang,Xueyan Yun,Jiushi Guo,Jian Hu,Lijun Song,Pengju Pan,Tungalag Dong
Incorporating inorganic particles is a common approach to preparing polymeric materials with desirable physical properties and processability. However, this often results in increased brittleness, necessitating methods to improve melt strength and toughness. In this study, in situ polymerization was employed to functionalize silica nanoparticles with the silane coupling agent (3-aminopropyl) triethoxysilane (APTES) as a core. A flexible chain segment, poly(butylene itaconate) (PBI), was then introduced as a “rubbery” intermediate layer, resulting in a core-shell structure of poly(L-lactic-co-butanediol itaconate) nano-silica copolymer films (PLBI SiO 2 ) with both branched and “rubbery” structures. Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR) confirmed the formation of macromolecular chains, with the molecular weight ( M n ) of PLBI increasing from 59,638 to 74,306 g/mol. This significant increase supports the “rubbery” core-shell structure. When 0.5% SiO 2 was added, the T 5% of the film increased by 40°C, significantly improving thermal stability. Additionally, the elongation at break increased to 265.7%, while retaining the original tensile strength. Dynamic rheology experiments further confirmed the generation of branched or “rubbery” core-shell structures, and a doubling of gas barrier properties was observed with increased silica nanoparticles, suggesting potential applications in food packaging or biopharmaceuticals. Highlights Nanocomposites with core-shell structure and improved mechanical properties. Dynamic rheology experiments confirmed the formation of the core-shell structure. Significantly improved gas barrier properties due to core-shell structure.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
·
IF 9.4
Jiawei Hu,Jiaming Li,Zhongyi Pu,Wen Xiao,Huan Yu,Zhihao Zhang,Fang Yu,Chao Liu,Qinfang Zhang
Heterostructured visible-light-responsive photocatalysts represent a prospective approach to achieve efficient solar-to-chemical energy conversion. Herein, we propose a facile self-assembly technique to synthesize NiO nanoparticles/C 3 N 5 nanosheets (NOCN) heterojunctions for hydrogen (H 2 ) evolution catalysis and hydrogen peroxide (H 2 O 2 ) production under visible light. In this regard, the black NiO nanoparticles (NPs) were tightly anchored on the surface of C 3 N 5 nanosheets (CNNS) to construct S-scheme NOCN heterojunction, enabling efficient charge separation and high redox capability. Obtained results elucidated that the incorporated NiO NPs significantly promote light-harvesting efficiency and photo-to-thermal capacity over the NOCN composites. The enhanced photothermal effect facilitates the charge carrier transfer rate across the heterojunction and boosts the surface reaction kinetics. Accordingly, the photocatalytic performances of CNNS for H 2 release and H 2 O 2 production can be manipulated by introducing NiO NPs. The modified photocatalytic properties of NOCN composites are ascribed to the synergistic effects of all integrated components and the S-scheme heterojunction formation. Impressively, the high H 2 evolution photocatalysis efficiency of NOCN nano-catalysts in seawater certifies their potential environmental applicability. Among all, the 12-NOCN nano-catalyst exhibits a higher photocatalytic efficiency for H 2 release (112.2 μmol∙g −1 ∙h −1 ) and H 2 O 2 production (91.2 μmol∙L −1 ∙h −1 ). Besides, the 12-NOCN nano-catalyst reveals excellent recyclability and structural stability. Additionally, the possible mechanism for photothermal-assisted photocatalysis is proposed. This work affords a feasible pathway to design photothermal-assisted S-scheme heterojunctions for diverse photocatalytic applications.
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ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xiaohui Niu,Yuewei Wang,Xing Yang,Yongqi Liu,Mei Yuan,Jianying Zhang,Hongxia Li,Kunjie Wang
Introducing chiral molecules into metal–organic frameworks (MOFs) to obtain chiral MOFs (CMOFs), the tunability of their structures makes them a highly anticipated class of chiral materials for electrochemical sensing. However, the structure of CMOFs is often limited by synthesis challenges, and introducing chiral molecules into MOFs often leads to a decrease in their internal space. This study introduces a defect engineering strategy into the synthesis of CMOFs to obtain CMOFs with defects, which is an efficient synthesis method. The two CMOFs constructed with different structures not only have more chiral recognition sites but also greatly increase the substrate capacity due to the defects, making them have a wide range of substrates and enhancing the enantioselective recognition effect of the two defective CMOFs. In addition, using MOF as a chiral carrier greatly overcomes the problem of low conductivity of chiral molecules. Based on the advantages of defective CMOFs, we have designed a novel chiral electrochemical sensor with an excellent enantiomer recognition performance. This study provides a simple and scalable synthetic method for constructing CMOFs with defects and high stability.
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FOOD CHEMISTRY
·
2024
·
IF 8.5
Linze Liu,Yajie Hu,Le Du,Jiahui Du,Jian Hu,Tao Sun,Tungalag Dong,Xueyan Yun
Antimicrobial packaging can prolong the shelf life of fresh food, but those active antimicrobial substances may leach into the food and affect its quality. To avoid this phenome, the C C double bond was first incorporated into the chemical structure of polylactic acid (PLLA) and tannic acid (TA) to prepare poly(L-LA- co -butyrate itaconate) (PLBI) and photoactive tannic acid (pTA). Then pTA-grafted PLBI (pTA-g-PLBI) films were fabricated using UV curing technology. Results showed that pTA was successfully grafted onto the surface of PLBI film and formed a uniform layer. The pTA-g-PLBI films exhibited good bacteriostatic effects of 86 %, 90 %, and 96 % on E. coli, P. fluorescens, and S. aureus , respectively. Additionally, pTA-g-PLBI packaging reduced the relative abundance of Shewanella , Psychrobacter, and Pseudomonas in chilled pork and delayed the deterioration of pork for more than 5 days.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Fen Ma,Jiali Jiao,Yuchen Chao,Wenyuan Xie,Defeng Wu
Rod-like chitin nanocrystals (ChNCs) filled biodegradable aliphatic polyesters are of great interest because as-obtained nanocomposites are all-degradable. In this work, we prepared a poly(ε-caprolactone- b - l -lactide) (PCL- b -PLA) copolyester nanocomposite with ChNCs and carried out a crystallization study. The results disclosed that the roles of ChNCs played during crystallization of copolyester matrices were very attractive: as heterogeneous nucleator of PLA phase, whereas as inert filler for PCL phase. Thus, the overall crystallization kinetics of PLA phase were accelerated. The heterogeneous nucleation led to the formation of more amounts of crystallized PLA domains, which favored nucleating following crystallization of PCL phase. In addition, the presence of ChNCs did not strongly influence the microphase-segregated structure of PCL- b -PLA, and had good reinforcements to matrix copolymer. The selective nucleation ability of ChNCs reported in this study is valuable for regulating the structures and properties of nanocomposites based on the ChNCs-filled aliphatic diblock copolyester with double crystalline blocks.
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JOURNAL OF FOOD SCIENCE
·
2025
·Vol. 90
·期 8
·e70445
·
IF 3.4
Jiahui Du,Yifeng Wu,Jiushi Guo,Jie Cao,Tao Sun,Tungalag Dong,Yuming Deng,Xing Wang,Xueyan Yun
The quality of yogurt deteriorates during storage due to exposure to oxygen and UV light, and high-barrier packaging effectively mitigates this issue. Concurrently, there is increasing interest in replacing petroleum-based packaging with eco-friendly alternatives. In this study, poly(lactic acid-butylene itaconate) (PLBI), developed as a modified version of poly (L-lactic acid) (PLLA) to enhance toughness, was combined with poly (vinyl alcohol) grafted with acryloyl chloride (PVA-g-AC) through UV curing. The resulting PLBI/PVA-g-AC films exhibited significantly improved barrier properties. They were used to package stirred yogurt alongside polyethylene/nylon (PE/NY) films for comparison. Quality assessments, including acidity, water-holding capacity, and viable bacterial counts, demonstrated that PLBI/PVA-g-AC films provided superior preservation. Metabolomics analysis revealed increased flavor compounds, such as acids and alcohols, and decreased sugar levels in these packaged yogurt. This work expands potential applications of PLLA-based materials in food packaging and offers insights into the influence of oxygen on yogurt storage quality.
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MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 12
·800
·
IF 5.3
Kalhoro Kashif Ali,Zhang Chi,Rehman Atiq Ur,Anwar Muhammad,Wu Di,Khan Adil,Kim Dokyoon,Shokouhimehr Mohammadreza,Liu Zhengchun
Layered coordination polymer nanoflakes of copper tetracyanonickelate CuNi(CN) 4 were synthesized as a bimetal nanocomposite by a facile co-precipitation technique at room temperature and applied to electrochemical glucose sensing, demonstrating strong potential as a non-enzymatic electrode material. The integration of Cu and Ni centers within a cyanide-bridged Prussian blue-type framework afforded abundant redox-active sites and a high surface area, facilitating efficient electron transfer and enhanced catalytic activity. Electrochemical analyses, including cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and chronoamperometry (CA), confirmed the electrode’s strong electrocatalytic response toward glucose oxidation. The CuNi(CN) 4 -modified electrode exhibited remarkable sensitivity, recording a response of 1151.48 μA mM −1 cm −2 in CV along with a detection limit of 0.35 μM. In CA analysis, an even greater sensitivity of 1796.53 μA mM −1 cm −2 was achieved across a glucose concentration range 0.1–12 mM, accompanied by excellent linearity (R 2 = 0.9989). Moreover, the sensor exhibited a rapid response, strong anti-interference capability, and operational stability maintained over 16 days. Practical applicability was validated through glucose quantification in commercial beverages, including black tea, Pepsi, and Sprite, yielding recoveries of 95.92%–103.52%, with RSD values consistently below 1.625%. These findings demonstrate the CuNi(CN) 4 nanocomposite’s promise as a reliable and high-performance platform for real-time glucose monitoring in food diagnostics. Graphical abstract
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ELECTROCHIMICA ACTA
·
2025
·
IF 5.6
Huimin Wang,Ruoxi Liu,Guangxin Wang,Minggang Liu,Jing Yu
An electrochemiluminescent enantioselective recognition platform was constructed by a defective homochiral MOF (Zn 2 (bdc)(L-lac)(dmf)), referred to as ZnBLD, exhibiting efficient ECL enantiodiscrimination for phenylalanine (Phe) through differences in ECL intensity. The synergistic effect of chiral transfer and defect induction endows it with excellent enantioselective recognition ability. In the system employing Ru(bpy) 3 2+ as the luminophore, this platform efficiently recognizes Phe across a broad concentration range from 10 –9 to 10 –2 M, achieving a limit of detection (LOD) of 3.3 × 10 –6 M. A high ECL intensity ratio (ECL L-Phe /ECL D-Phe ) of 2.8 was observed at 10 –4 M. The recognition mechanism stems from the synergistic interaction of three factors, including the increased chiral active sites, an enlarged pore size induced by defects, and favorable electrostatic interactions. Specifically, the spatial adaptability of chiral sites provides a specific recognition environment for l-Phe enantiomers, enabling preferential binding to target enantiomers through stereoconfigurational matching; the defect structures in MOFs not only increase the number of active recognition sites but also enhance molecular mass transfer efficiency by enlarging pore sizes, strengthening effective contact with l-Phe and thus improving recognition efficiency; meanwhile, electrostatic interactions between l-Phe and ZnBLD further stabilize the binding system, promoting the formation of stable diastereoisomer complexes and synergistically enhancing ECL efficiency. This research develops a novel ECL enantiodiscrimination strategy based on chiral defective MOFs, providing new insights and possibilities for chiral recognition sensing.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Wenhui Qu,Luying Tang,Ruoshu Li,Weijia Liu,Xiaoming Jiang,Qing Kong,Yunqi Wen,Changhu Xue
Antarctic krill, a shrimp-like crustacean, whose quality strongly influences the quality of fermented shrimp sauce, yet the relationship and prediction methods remain unclear. This study evaluated 75 krill samples of varying freshness and their corresponding shrimp sauces, and successfully developed a high-performance predictive model linking raw material quality to product quality. Shrimp spoiled significantly after only 24 h at room temperature, exhibiting darkened color, increased spoilage-related compounds, with yield and conversion rate sharply decreasing from 80.4 % and 87.1 % to 58.9 % and 63.7 %, respectively. Machine learning with multiple feature selection methods and models showed that Lasso regression effectively reduced collinearity, and its integration with a linear model enhanced prediction accuracy, with nearly 80 % of features well-fitted and about 70 % showing mean absolute percentage error below 5 %. This study successfully established an accurate model linking krill freshness to shrimp sauce quality, which offered guidance for krill utilization and intelligent fermentation.
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