CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Zhiwei Chen,Zheng Zhao,Jiaming Yuan,Qiaoxuan Zhang,Yufeng Wu,Qingjin Wu,Yuheng Liao,Jia-Huan Qu,Ning Gan,Zhengjin Jiang,Qiqin Wang
Polymeric supramolecular assemblies (PSAs) exhibit excellent chemical stability, mechanical integrity, and multifunctionality, which could drive the development of biosensors. However, trapped by the significant differences in the physicochemical properties and structures of recognition ligands and signal tags, PSAs that simultaneously carry ligands and signals have rarely been reported for bioanalysis. In this study, an amphiphilic block copolymer (PVIM-b-PFMMA) was controllably synthesized by reversible addition fragmentation chain transfer (RAFT) radical polymerization for the first time. The resultant copolymer can self-assemble into highly electroactive supramolecular nanoprobes (eSNPs) with integrated target recognition and signal amplification functions. Compared to traditional electroactive probes based on conductive nanomaterials or enzyme catalysis, these versatile eSNPs avoid disadvantages including high cost, tedious modification, low load capacity and leakage of signal tags. For the rapid, sensitive and accurate monitoring of rituximab in biological fluids, a new electrochemical biosensor was constructed by coupling eSNPs with mimotope peptide CN14-modified screen-printed gold electrodes. The developed biosensor achieved a limit of detection (LOD) as low as 25 pg/mL and was successfully used to monitor rituximab concentration in a series of serum samples from non-Hodgkon’s lymphoma patients. Compared with commercial enzyme-linked immunosorbent assay (ELISA) and previously reported methods, the proposed biosensor demonstrated higher sensitivity, lower cost, simpler operation and more efficient preparation, owing to the unique superiority of eSNPs. Overall, this study presents a promising pathway for developing PSAs-based biosensors and their applications in bioanalysis.
研究领域:
生物传感器
·
纳米技术
·
电化学
·
癌症诊断
·
聚合物材料
✓ 已验证
产品关联
DOI
高影响因子
VIBRATIONAL SPECTROSCOPY
·
2021
·
IF 2.507
Lei Zhang,Yingshan Chen,Qin Zhu,Wenjin Ji,Suqing Zhao
Bisphenol A (BPA) is a typical endocrine-disrupting chemical (EDC), which has been banned for food packing and baby bottle material due to its potential endocrine harm to human body. In this paper, an easy and rapid immunochromatographic assay (ICA) combined with the technology of surface-enhanced Raman spectroscopy (SERS) was fabricated to improve the sensitivity and rapidity for quantitative determination of BPA. After 10 min of a quick chromatographic process, the specific Raman signal on T line of the test strip was measured. Compared with the traditional ICA, the SERS-ICA realized quantitative test and showed the limit of detection (LOD) was 0.1 ng/mL, getting approximately 300-fold lower. The method displayed high specificity and precision with RSD of 4.1%, and the test strip indicated favorable stability for two weeks without loss of activity. For river, tap and commercial drinking water sample, the SERS-ICA results were confirmed by HPLC, and the recoveries of spiked BPA were from 90.80-117.80%. It was shown that the proposed method takes advantages both of SERS and ICA, exhibiting high sensitivity, precision, stability, quantification and rapidity for field test, expecting a widely useful method for various small molecules in food safety, medical diagnosis and environmental samples.
研究领域:
分析化学
·
环境科学
·
食品安全
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2020
·Vol. 44
·期 17
·6992-7000
·
IF 3.288
Tehseen Nawaz,Muhammad Ahmad,Jieying Yu,Shiqi Wang,Tianxin Wei
Novel bifunctional group monomer itaconic acid (IA) based molecularly imprinted polymers (MIPs) were successfully prepared with hydrogen bonding interactions by applying a facile combination of a reversible addition chain transfer mechanism (RAFT) and surface plasmon resonance (SPR). UV photo-polymerization in synergy with 2-methyl-2-[(dodecylsulfanylthiocarbonyl)sulfanyl]propanoic acid (TTCA) as a chain transfer reagent was employed for MIP film synthesis on an SPR sensor chip for the detection of progesterone using ethylene glycol dimethacrylate (EGDMA) as a cross linker. In addition, the ratio of template to bifunctional group monomer to cross linker was optimized (1 : 2 : 12) by real-time SPR monitoring. The modified surface of the biosensor was characterized by contact angle measurements, frontier transfer infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The resulting itaconic acid based MIP (IA based MIP) biosensor showed a high adsorption capacity and excellent selectivity in comparison to other analogues and non-imprinted polymer (NIP) film. Through 8 adsorption–desorption cycles, the high recoverability of IA based MIP film was confirmed. These features suggested that IA based MIPs are ideal candidates for biosensor use owing to the bifunctionality of the monomer. Consequently, within the concentration range of 1 × 10−18 to 1 × 10−8 mol L−1, the coupling angle change of SPR versus the negative logarithm of concentration showed excellent linearity: R2 = 0.99. Based on a linear equation, IA based MIPs showed excellent values for the limit of detection (LOD) and limit of quantification (LOQ), i.e. 0.28 × 10−19 mol L−1 and 0.92 × 10−19 mol L−1, respectively. Furthermore, the influence of the matrix on the biosensor was successfully analyzed in real samples with satisfactory outcomes, displaying identical behavior to ideal samples. Hence, IA based MIPs in combination with an SPR sensor chip demonstrated potential applications for rapid and highly effective sensing even in complicated matrices.
研究领域:
分析化学
·
生物传感器
·
高分子科学
·
材料科学
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生物技术
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 5
·1-13
·
IF 5.3
Gao Xue,Liu Lu,Jia Mu,Zhang Hongmei,Li Xuepeng,Li Jianrong
A dual-mode (colorimetric/fluorescence) nanoenzyme-linked immunosorbent assay (NLISA) was developed based on Au-Cu nanocubes generating Prussian blue nanoparticles (PBNPs). It is expected that this method can be used to detect the residues of sulfonamides in the field, and solve the problem of long analysis time and high cost of the traditional method. Sulfadimethoxine (SDM) was selected as the proof-of-concept target analyte. The Au-Cu nanocubes were linked to the aptamer by amide interaction, and the Au-Cu nanocubes, SDM and antibody were immobilized on a 96-well plate using the sandwich method. The assay generates PBNPs by oxidising the Cu shells on the Au-Cu nanocubes in the presence of hydrochloric acid, Fe 3+ and K 3 [Fe (CN) 6 ]. In this process, the copper shell undergoes oxidation to Cu 2+ and subsequently Cu 2 + further quenches the fluorescence of the carbon point. PBNPs exhibit peroxidase-like activity, oxidising 3,3’,5,5’-tetramethylbenzidine (TMB) to OX-TMB in the presence of H 2 O 2 , which alters the colorimetric signal. The dual-mode signals are directly proportional to the sulfadimethoxine concentration within the range 10 − 3 ~10 − 7 mg/mL. The limit of detection (LOD) of the assay is 0.023 ng/mL and 0.071 ng/mL for the fluorescent signal and the colorimetric signal, respectively. Moreover, the assay was successfully applied to determine sulfadimethoxine in silver carp, shrimp, and lamb samples with satisfactory results.
研究领域:
分析化学
·
纳米技术
·
生物化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Lishuang Wang,Longshan Zhao
A novel dual-emission fluorescence molecularly imprinted polymers (FL-MIPs) sensor was firstly constructed by mixing the green-emission fluorescence molecularly imprinted polymers (g-MIPs) and red-emission CdS/CdTe QDs (r-QDs) in proper proportion, which has been realizing the naked-eye detection of bovine serum albumin (BSA). The fluorescence of g-MIPs gradually turned off and red fluorescence remained after combining with BSA. The thickness of imprinted layer and the ratio of g-MIPs and r-QDs were investigated in detail so as to achieve the optimal performances of FL-MIPs sensor. Meanwhile, the Box-Behnken statistical design was used to optimize the crucial parameters that had an impact on the adsorption effect. Under optimal conditions, the FL-MIPs sensor had excellent linear relationship within 2–64 μM, and showed the fluorescence color changing from yellow-green to yellow to light pink to red, which achieved the visual detection. The outstanding visual effect of the FL-MIPs sensor made the detection of BSA convenient without too many requirements for equipment and the level of testing personnel except a portable ultraviolet lamp. The limit of detection (LOD) of BSA was 0.5 μM lower than that of most reported methods. Furthermore, the FL-MIPs sensor can successfully be used to detect BSA in milk, milk powder, human serum and calf serum, with the prefect spiked recoveries of 95.9–104.8%. The numerous advantages make the FL-MIPs sensor have great applicable potential in various fields.
研究领域:
材料科学
·
分析化学
·
生物传感器
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2023
·
IF 4.3
Chu Shushu,Xia Mingyuan,Xu Peng,Lin Dalei,Jiang Yuanyuan,Lu Yizhong
Although traditional Fe-based nanozymes have shown great potential, generally only a small proportion of the Fe atoms on the catalyst’s surface are used. Herein, we synthesized single-atom Fe on N-doped graphene nanosheets (Fe-CNG) with high atom utilization efficiency and a unique coordination structure. Active oxygen species including superoxide radicals (O 2 •− ) and singlet oxygen ( 1 O 2 ) were efficiently generated from the interaction of the Fe-CNG with dissolved oxygen in acidic conditions. The Fe-CNG nanozymes were found to display enhanced oxidase-like and laccase-like activity, with V max of 2.07 × 10 −7 M∙S −1 and 4.54 × 10 −8 M∙S −1 and K m of 0.324 mM and 0.082 mM, respectively, which is mainly due to Fe active centers coordinating with O and N atoms simultaneously. The oxidase-like performance of the Fe-CNG can be effectively inhibited by ascorbic acid (AA) or hydroquinone (HQ), which can directly obstruct the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). Therefore, a direct and sensitive colorimetric method for the detection of AA and HQ activity was established, which exhibited good linear detection and limit of detection (LOD) of 0.048 μM and 0.025 μM, respectively. Moreover, a colorimetric method based on the Fe-CNG catalyst was fabricated for detecting the concentration of AA in vitamin C. Therefore, this work offers a new method for preparing a single-atom catalyst (SAC) nanozyme and a promising strategy for detecting AA and HQ.
研究领域:
催化
·
分析化学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2020
·
IF 2.877
Ao Li,Peng Zuo,Bang-Ce Ye
Salmonella, a typical foodborne pathogen, always seriously threatens the health and even life of both humans and animals. However, highly sensitive and fast quantitative methods for its detection are remaining to be challenged. Herein, we presented an efficient method with dual signal amplification strategy by combining immune hybridization chain reaction (HCR) with surface enhanced Raman scattering (SERS) to high sensitively detect Salmonella typhimurium in food. After sample preparation, S. typhimurium were specifically captured by immunomagnetic beads (IMBs), then aptamers and hairpin-probes were added to trigger HCR to form nicked dsDNA, finally 4’,6-Diamidino-2-phenylindole dihydrochloride (DAPI) was incubated with HCR products and then the whole system was mixed with AgNP colloid to detect the SERS intensity at 1610 cm −1 . As a result, a good linear relationship was achieved between SERS intensities and corresponding concentrations of S. typhimurium ranging from 10 to 10 5 CFU/mL, with a limit of detection (LOD) of 6 CFU/mL in 3.5 h. The proposed method has been successfully applied to capture and detect the S. typhimurium in spiked milk samples, and the results were consistent with those of the traditional plate counting method. The method, with combination of HCR and SERS, achieves double amplification of the detection signal and significantly improves the detection sensitivity of S. typhimurium . And it also shows good application potential for the highly sensitive detection of other contaminants in food.
研究领域:
食品科学
·
生物传感器
·
病原体检测
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2023
·
IF 4.1
Jie Dong,Laixi Zhang,Wei Li,Xin Hu,Anyi Chen,Chaorui Li
In this study, a facile and cost-effective hydrothermal synthesis method was used to synthesize zinc oxide nanoflowers modified by reduced graphene oxide, and subsequently, trimetallic AuPtRu nanoparticles(AuPtRuNPs) were supported via the reduction method for high-sensitivity colorimetric detection of H2O2 in weakly acidic solutions. Compared to monometallic and bimetallic nanoparticles, trimetallic nanoparticles exhibit significant synergistic effects and enhanced catalytic activity. After providing a three-dimensional structure with multiple pores by zinc oxide and enhancing electron transfer ability by reduced graphene, the trimetallic nanocomposites (AuPtRu/ZnO-rGO) exhibited excellent peroxidase-mimicking activity, which can effectively catalyze 3,3′,5,5′-tetramethylbenzidine (TMB) to produce a blue oxidation product (oxTMB) in the presence of H2O2. Compared to horseradish peroxidase (HRP), AuPtRu/ZnO-rGO demonstrated significantly enhanced catalytic velocity (Vmax = 6.16 × 10–8 M/s) and affinity (Km = 0.02) for H2O2. The study of the catalytic mechanism showed that trimetallic Au, Pt, and Ru could effectively catalyze H2O2 to produce hydroxyl radicals (•OH) to accelerate the oxidation of TMB and enhance the peroxidase-mimicking activity of the AuPtRu/ZnO-rGO nanocomposites. The results showed that the as-synthesized hydrangea-like AuPtRu/ZnO-rGO nanocomposites showed enhanced peroxidase-mimicking activity. It could be used for the colorimetric detection of H2O2 in the range 5–1000 μM with a LOD of 3.0 μM (S/N = 3), and the recoveries are 93.0–101.7%. In addition, the AuPtRu/ZnO-rGO nanocomposites have good applicability for sensitive colorimetric determination of H2O2 in milk, and it has broad application prospects as a multifunctional sensing platform in the food processing industry.
研究领域:
材料科学
·
纳米技术
·
分析化学
·
生物催化
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Huihui Wang,Lu Cheng,Shang Ma,Liming Ding,Wei Zhang,Zhuobin Xu,Dandan Li,Lizeng Gao
Inspired by the particularity of tumor microenvironments, including acidity and sensibility to reactive oxygen species (ROS), advanced and smart responsive nanomaterials have recently been developed. The present study synthesized tumor-targeted and pH-sensitive supramolecular micelles that self-assembled via host–guest recognition. The micelles consumed intratumoral glucose and lactate via loading with glucose oxidase (GOD) and lactate oxidase (LOD). Intratumoral glucose and lactate were converted into hydrogen peroxide (H2O2) and were sequentially reduced to highly toxic hydroxyl radicals (•OH) via the peroxidase (POD)-like activity of the loaded C-dot nanozymes. Tumor-killing effects were observed via cascade catalytic reactions. After an intravenous injection, the nanocomposite exhibited an excellent tumor-targeted ability with good biocompatibility, which demonstrated its effective antitumor effect. The nanocomposite effectively combined starvation and catalytic therapies and exerted a synergistic anticancer effect with minimal side effects and without external addition.
研究领域:
纳米技术
·
生物医学工程
·
癌症治疗
·
生物化学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2022
·
IF 5.76
Yun Ni,Hezhong Ouyang,Lulu Yu,Chen Ling,Ziye Zhu,Aolin He,Ruijiang Liu
A label-free electrochemical aptasensor based on magnetic α-Fe 2 O 3 /Fe 3 O 4 heterogeneous hollow nanorods was developed for the detection of cancer antigen 125 (CA125). Magnetic α-Fe 2 O 3 /Fe 3 O 4 heterogeneous hollow nanorods were successfully prepared by the hydrolysis-calcination method, functionalized with polyethyleneimine (PEI), and modified with HAuCl 4 to form magnetic α-Fe 2 O 3 /Fe 3 O 4 -Au nanocomposites with a layer of 5 nm gold nanoparticles (Au NPs) on the surface. The magnetic α-Fe 2 O 3 /Fe 3 O 4 -Au nanocomposites were used to fix the DNA-aptamer probe to amplify the current signal. The intensity of the current signal was proportional to the concentration of CA125, indicating that the sensor was a turn-on sensor. The linear range of the electrochemical aptasensor was 5–125 U/mL ( R 2 = 0.9975), and the limit of detection (LOD) was 2.99 U/mL. The electrochemical aptasensor exhibited favorable specificity, reproducibility, and stability. The analytical performance of the aptasensor in real serum samples was also investigated.
研究领域:
生物化学
·
纳米技术
·
分析化学
·
生物传感器
·
癌症诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LAB ON A CHIP
·
2023
·Vol. 23
·期 10
·2477-2486
·
IF 6.1
Xiaoyi Li,Zhihao Feng,Changxiang Fang,Yunpeng Wei,Dandan Ji,Weihua Hu
Oblique-incidence reflectivity difference (OIRD) is a compelling technique for real-time, label-free and non-destructive detection of antibody microarray chips, but its sensitivity needs essential improvement for clinical diagnosis. In this study, we report an innovative high-performance OIRD microarray by using poly[oligo(ethylene glycol) methacrylate-co-glycidyl methacrylate] (POEGMA-co-GMA) brush grafted fluorine-doped tin oxide (FTO) as the chip substrate. The polymer brush enhances the interfacial binding reaction efficiency of targets from the complicated sample matrix due to its high antibody loading and excellent anti-fouling merits; the FTO-polymer brush layered structure, on the other hand, excites the interference enhancement effect of OIRD to achieve enhanced intrinsic optical sensitivity. Synergistically, the sensitivity of this chip is significantly improved compared to rival chips, achieving a limit of detection (LOD) as low as 25 ng mL−1 for the model target C-reactive protein (CRP) in 10% human serum. This work explores the tremendous influence of the chip interfacial structure on the OIRD sensitivity and proposes a rational interfacial engineering strategy to boost the performance of the label-free OIRD based microarray and other bio-devices.
研究领域:
材料科学
·
生物医学工程
·
分析化学
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Ruijiang Liu,Shuai Pan,Min Liu,Wei Huang,Zhixiang Lv,Aolin He
A label-free electrochemical biosensor with magnetically induced self-assembly based on peptide nucleic acids (PNA) and Fe 3 O 4 /Fe 2 O 3 heteroplasmon nanorods was successfully fabricated to detect the single nucleotide polymorphism (SNP) of CYP2C9*3. The sensitivity of the electrochemical biosensor was improved by introducing PNA and Fe 3 O 4 /Fe 2 O 3 @Au nanocomposites. The Fe 3 O 4 /Fe 2 O 3 @Au nanocomposites were assembled on the surface of the magnetic glassy carbon electrode (MGCE) by magnetic field, and the thiolated PNA were connected to the nanocomposites through Au-S bonds. The biosensor exhibited a good linear correlation between 1 pM and 1 µM (R 2 = 0.9941), and the limit of detection (LOD) and the limit of quantitation (LOQ) were 0.95 pM and 3.18 pM, respectively. Besides, the electrochemical biosensor could recognize single-base mismatch DNA, and it could be stored stably for 15 days at 4 °C.
研究领域:
生物传感器
·
电化学
·
分子诊断
·
纳米技术
·
遗传学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）